AT5G37640

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020822
Physical Location & Seq
Reverse (-)
14952782 .. 14953750
969 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37640.1

Sequence Viewer

Length: 969 bp
ATGTCGATGCAAATTCACGCAAAGACTCTCACAGAAAAGACCATAACCATTGACGTTGTAAGCTCCGACACCATCAATAATGTCAAAGCAAAGATCCAGGACATAGAAGGGATTCCTCTAGACCAGCAAAGACTCATCTTTTCCGGTAAACTGCTAGATGATGGCCGTACTTTGGCCGATTACAGTATCCAAAAAGATTCAATACTCCACTTAGCTCTCAGGTTGAGAGGTGGTATGCAGATATTTGTCAAGACGCTTACCGGTAAAACCATTACCCTTGAAGTTGAGAGTTCAGACACTATTGACAATGTTAAAGCTAAGATCCAAGACAAGGAAGGAGTCCCTCCGGATCAACAGAGACTAATCTTTGCTGGTAAACAGCTTGACGATGGTCGCACTCTTGCAGATTACAACATCCAGAAAGAATCAACTCTCCACTTGGTTCTCAGGTTGAGAGGTGGTATGCAGATTTTTGTCAGGACACTTACCCGAAAAACCATAGCTTTGGAAGTTGAGAGCTCTGACACAACCGATAATGTGAAGGCCAAGATACAAGACAAAGAAGGGATCCCACCAGACCAACAGAGACTTATCTTTGCAGGCAAACAACTCGAAGATGGTCGGACGCTTGCTGATTACAACATCCAGAAGGAGTCAACACTTCATCTCGTGCTGCGTCTTTGTGGTGGGATGCAGATATTTGTCAACACTTTGACGGGAAAGACCATCACACTTGAAGTGGAGAGCTCTGATACGATTGACAATGTGAAGGCCAAGATTCAGGACAAAGAAAGGATCCAACCGGACCAACAGAGGCTAATCTTTGCAGGCGAGCAGCTTGAAGATGGTTATTACACTTTAGCGGATTACAATATCCAGAAAGAGTCAACTCTTCACCTTGTTCTCCGTCTCCGCGGTGGAGAGTGCTTTGGTTTTATTTTTCTTTTCTTGTTATGTTTTAACTCTTGA

Protein Analysis

322

Amino Acids

36.26

Weight (kDa)

5.83

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 3 - 71 3.2e-11 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 8 - 76 6.2e-24 Ubiquitin family
Rad60-SLD PF11976 79 - 149 1.5e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 81 - 152 1.8e-31 Ubiquitin family
Rad60-SLD PF11976 155 - 225 1.2e-14 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 157 - 227 9.3e-29 Ubiquitin family
Rad60-SLD PF11976 231 - 302 1.7e-12 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 233 - 305 1.8e-25 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020822)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37640
malus_domestica MD11G1226200.v1.1
prunus_persica Prupe.4G204900_v2.0.a1 Prupe.4G204900_v2.0.a1
pyrus_communis pycom10g26700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 915
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 3 cut(s) 863, 913, 915
AclWI GGATC 7 cut(s) 88, 316, 357, 562, 575, 790, 803
AcoI YGGCCR 2 cut(s) 163, 174
AcsI RAATTY 1 cut(s) 12
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 2 cut(s) 172, 331
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 4 cut(s) 201, 281, 737, 842
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 8 cut(s) 63, 215, 317, 382, 503, 519, 747, 838
AluI AGCT 8 cut(s) 63, 215, 317, 382, 503, 519, 747, 838
Alw21I GWGCWC 2 cut(s) 521, 749
Alw26I GTCTC 3 cut(s) 352, 580, 914
AlwI GGATC 7 cut(s) 88, 316, 357, 562, 575, 790, 803
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 4 cut(s) 163, 174, 543, 771
ApeKI GCWGC 2 cut(s) 673, 835
ApoI RAATTY 1 cut(s) 12
AsiGI ACCGGT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 805
AsuHPI GGTGA 1 cut(s) 887
AvaII GGWCC 1 cut(s) 805
BamHI GGATCC 2 cut(s) 567, 795
BanII GRGCYC 2 cut(s) 521, 749
BauI CACGAG 1 cut(s) 668
Bbv12I GWGCWC 2 cut(s) 521, 749
BbvI GCAGC 2 cut(s) 660, 847
BccI CCATC 6 cut(s) 80, 155, 383, 611, 734, 839
BceAI ACGGC 1 cut(s) 150
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BciT130I CCWGG 1 cut(s) 98
BciVI GTATCC 1 cut(s) 197
BcoDI GTCTC 3 cut(s) 352, 580, 914
BfaI CTAG 2 cut(s) 119, 155
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 674, 836
BlsI GCNGC 2 cut(s) 675, 837
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 805
BmiI GGNNCC 2 cut(s) 569, 797
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 681
BoxI GACNNNNGTC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 913
BsaWI WCCGGW 4 cut(s) 143, 260, 346, 802
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 331
Bse118I RCCGGY 1 cut(s) 260
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 913
BseGI GGATG 3 cut(s) 414, 642, 696
BseLI CCNNNNNNNGG 2 cut(s) 172, 331
BseMII CTCAG 2 cut(s) 232, 460
BseXI GCAGC 2 cut(s) 660, 847
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 4 cut(s) 165, 176, 545, 773
BshTI ACCGGT 1 cut(s) 260
BsiHKAI GWGCWC 2 cut(s) 521, 749
BsiSI CCGG 4 cut(s) 144, 261, 347, 803
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 172, 331
BsmAI GTCTC 3 cut(s) 352, 580, 914
BsmBI CGTCTC 1 cut(s) 914
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 4 cut(s) 165, 176, 545, 773
Bsp1286I GDGCHC 2 cut(s) 521, 749
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 5 cut(s) 93, 321, 349, 567, 795
BspACI CCGC 3 cut(s) 863, 913, 915
BspANI GGCC 4 cut(s) 165, 176, 545, 773
BspCNI CTCAG 2 cut(s) 231, 459
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 2 cut(s) 569, 797
BspPI GGATC 7 cut(s) 88, 316, 357, 562, 575, 790, 803
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 913
BssMI GATC 5 cut(s) 93, 321, 349, 567, 795
BssSI CACGAG 1 cut(s) 668
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 897
BstC8I GCNNGC 4 cut(s) 601, 630, 829, 833
BstDEI CTNAG 4 cut(s) 211, 218, 318, 446
BstDSI CCRYGG 1 cut(s) 913
BstF5I GGATG 3 cut(s) 414, 642, 696
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 5 cut(s) 96, 324, 352, 570, 798
BstMAI GTCTC 3 cut(s) 352, 580, 914
BstMBI GATC 5 cut(s) 93, 321, 349, 567, 795
BstNI CCWGG 1 cut(s) 98
BstPAI GACNNNNGTC 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 2 cut(s) 660, 847
BstX2I RGATCY 4 cut(s) 93, 321, 567, 795
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 4 cut(s) 93, 321, 567, 795
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 4 cut(s) 165, 176, 545, 773
BtgI CCRYGG 1 cut(s) 913
BtsCI GGATG 3 cut(s) 414, 642, 696
Cac8I GCNNGC 4 cut(s) 601, 630, 829, 833
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 805
Cfr42I CCGCGG 1 cut(s) 916
CseI GACGC 3 cut(s) 262, 634, 665
Csp6I GTAC 1 cut(s) 168
CspAI ACCGGT 1 cut(s) 260
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 4 cut(s) 211, 218, 318, 446
DpnI GATC 5 cut(s) 95, 323, 351, 569, 797
DpnII GATC 5 cut(s) 93, 321, 349, 567, 795
EaeI YGGCCR 2 cut(s) 163, 174
Eam1104I CTCTTC 1 cut(s) 897
EarI CTCTTC 1 cut(s) 897
Ecl136II GAGCTC 2 cut(s) 519, 747
Eco24I GRGCYC 2 cut(s) 521, 749
Eco47I GGWCC 1 cut(s) 805
Eco53kI GAGCTC 2 cut(s) 519, 747
EcoICRI GAGCTC 2 cut(s) 519, 747
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 2 cut(s) 521, 749
Esp3I CGTCTC 1 cut(s) 914
FaiI YATR 6 cut(s) 44, 104, 236, 464, 500, 955
FaqI GGGAC 1 cut(s) 326
Fnu4HI GCNGC 2 cut(s) 674, 836
FokI GGATG 3 cut(s) 401, 629, 703
FriOI GRGCYC 2 cut(s) 521, 749
Fsp4HI GCNGC 2 cut(s) 674, 836
FspBI CTAG 2 cut(s) 119, 155
GluI GCNGC 2 cut(s) 674, 836
HaeIII GGCC 4 cut(s) 165, 176, 545, 773
HapII CCGG 4 cut(s) 144, 261, 347, 803
HgaI GACGC 3 cut(s) 262, 634, 665
HincII GTYRAC 3 cut(s) 657, 706, 888
HindII GTYRAC 3 cut(s) 657, 706, 888
HinfI GANTC 9 cut(s) 25, 112, 132, 197, 339, 425, 653, 778, 884
HpaII CCGG 4 cut(s) 144, 261, 347, 803
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 5 cut(s) 149, 377, 657, 706, 888
Hpy188I TCNGA 5 cut(s) 67, 295, 523, 624, 751
Hpy188III TCNNGA 9 cut(s) 119, 250, 347, 418, 478, 646, 782, 877, 966
Hpy8I GTNNAC 5 cut(s) 149, 377, 657, 706, 888
HpyAV CCTTC 6 cut(s) 101, 329, 535, 557, 643, 763
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 7 cut(s) 10, 238, 404, 466, 599, 694, 827
HpyF3I CTNAG 4 cut(s) 211, 218, 318, 446
HpySE526I ACGT 1 cut(s) 54
Kpn2I TCCGGA 1 cut(s) 346
KspI CCGCGG 1 cut(s) 916
Kzo9I GATC 5 cut(s) 93, 321, 349, 567, 795
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 2 cut(s) 660, 847
LweI GCATC 1 cut(s) 681
MaeI CTAG 2 cut(s) 119, 155
MaeII ACGT 1 cut(s) 54
MalI GATC 5 cut(s) 95, 323, 351, 569, 797
MboI GATC 5 cut(s) 93, 321, 349, 567, 795
MboII GAAGA 3 cut(s) 626, 854, 884
MflI RGATCY 4 cut(s) 93, 321, 567, 795
MhlI GDGCHC 2 cut(s) 521, 749
MluCI AATT 1 cut(s) 12
MlyI GAGTC 5 cut(s) 19, 126, 348, 662, 893
MmeI TCCRAC 3 cut(s) 90, 602, 823
MnlI CCTC 5 cut(s) 126, 221, 354, 449, 807
MroI TCCGGA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 111
MseI TTAA 2 cut(s) 312, 960
MspA1I CMGCKG 1 cut(s) 915
MspI CCGG 4 cut(s) 144, 261, 347, 803
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 915
NdeII GATC 5 cut(s) 93, 321, 349, 567, 795
NlaIV GGNNCC 2 cut(s) 569, 797
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 4 cut(s) 112, 197, 425, 778
PfoI TCCNGGA 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 2 cut(s) 675, 837
PleI GAGTC 5 cut(s) 19, 126, 347, 661, 892
PpsI GAGTC 5 cut(s) 19, 126, 347, 661, 892
PshAI GACNNNNGTC 1 cut(s) 390
Psp124BI GAGCTC 2 cut(s) 521, 749
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 569, 797
PspPI GGNCC 1 cut(s) 805
PsuI RGATCY 4 cut(s) 93, 321, 567, 795
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SacI GAGCTC 2 cut(s) 521, 749
SacII CCGCGG 1 cut(s) 916
SaqAI TTAA 2 cut(s) 312, 960
SatI GCNGC 2 cut(s) 674, 836
Sau3AI GATC 5 cut(s) 93, 321, 349, 567, 795
Sau96I GGNCC 1 cut(s) 805
SchI GAGTC 5 cut(s) 19, 126, 348, 662, 893
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 2 cut(s) 521, 749
SfaNI GCATC 1 cut(s) 681
Sfr303I CCGCGG 1 cut(s) 916
SgrBI CCGCGG 1 cut(s) 916
SinI GGWCC 1 cut(s) 805
Sse9I AATT 1 cut(s) 12
SsiI CCGC 3 cut(s) 863, 913, 915
SspMI CTAG 2 cut(s) 119, 155
SstI GAGCTC 2 cut(s) 521, 749
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 5, 612
TasI AATT 1 cut(s) 12
TfiI GAWTC 4 cut(s) 112, 197, 425, 778
Tru1I TTAA 2 cut(s) 312, 960
Tru9I TTAA 2 cut(s) 312, 960
TseI GCWGC 2 cut(s) 673, 835
TspDTI ATGAA 1 cut(s) 653
TspGWI ACGGA 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 805
XapI RAATTY 1 cut(s) 12
XbaI TCTAGA 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 111
XspI CTAG 2 cut(s) 119, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.