MD11G1226200.v1.1

Ubiquitin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0020822
Physical Location & Seq
Reverse (-)
33012063 .. 33012623
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1226200.v1.1.491

Sequence Viewer

Length: 561 bp
ATGCAAATCTTTGCCAAGATGCTAACCGGAAAGACCATCACCTTGGAAGTTGAAAGCTCGGATACCATTGATAATGTGAAGGCAAAGATCCAGGACAAGAAGTCGACTCTCCACTTGGTGCTCCAACTGCGTGGTGGTATGCAAATCTTCGTGAAGACCTTGACCGGGAAGACCATCACTCTGGAAGTTGAAAGTTCTGAGGGCATTCCCCCAGACCAGCAGCGGTTGATCTTTGCAGGTAAGCAGCTTGAGGATGGGAGGACACTTGCTGATTACAACATTCAAAAGGAGTCCATCCTGCACCTGGTAATTCTTCTGCGTGGTGGAATGCAGATCTTTGTGAAGACATTGACTGGGAAGACGATAACGTTGGAGGTAGAGAGTTGTGATACCATTGATAATGTGAAAGCAAAGATCCAGGACAAGGAGGGCATTCCTCCGGACCAGCAGAGACTCATCTTTGCCGGTAAGCAGCTGGAGGACGGAAGGACTCTTGCGGATTACAACATCCAAAAGGAGTCTACCCTTCACCTTGTCCTTCGCCTCCGTGATGGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

187

Amino Acids

20.83

Weight (kDa)

6.84

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 3 - 34 3.5e-06 Ubiquitin family
ubiquitin PF00240 53 - 105 6.6e-17 Ubiquitin family
Rad60-SLD PF11976 66 - 104 2.4e-08 Ubiquitin-2 like Rad60 SUMO-like
Rad60-SLD PF11976 110 - 180 1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 112 - 183 3.7e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020822)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37640
malus_domestica MD11G1226200.v1.1
prunus_persica Prupe.4G204900_v2.0.a1 Prupe.4G204900_v2.0.a1
pyrus_communis pycom10g26700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 227
AccI GTMKAC 2 cut(s) 104, 521
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 2 cut(s) 223, 497
AclI AACGTT 1 cut(s) 368
AclWI GGATC 2 cut(s) 82, 409
AdeI CACNNNGTG 1 cut(s) 118
AfiI CCNNNNNNNGG 3 cut(s) 165, 304, 424
AgsI TTSAA 3 cut(s) 53, 191, 284
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 3 cut(s) 90, 303, 417
AluBI AGCT 3 cut(s) 57, 247, 475
AluI AGCT 3 cut(s) 57, 247, 475
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 82, 409
Aor13HI TCCGGA 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 220, 244, 472
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 31, 521
AvaII GGWCC 1 cut(s) 442
BbsI GAAGAC 4 cut(s) 161, 176, 350, 365
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 232, 256, 484
BccI CCATC 5 cut(s) 44, 182, 248, 302, 545
BciT130I CCWGG 3 cut(s) 92, 305, 419
BciVI GTATCC 1 cut(s) 55
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 227
BfuI GTATCC 1 cut(s) 55
BglII AGATCT 1 cut(s) 333
BisI GCNGC 3 cut(s) 221, 245, 473
BlsI GCNGC 3 cut(s) 222, 246, 474
Bme1390I CCNGG 4 cut(s) 92, 166, 305, 419
Bme18I GGWCC 1 cut(s) 442
BmeRI GACNNNNNGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 442
BmrFI CCNGG 4 cut(s) 92, 166, 305, 419
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 4 cut(s) 161, 176, 350, 365
BpmI CTGGAG 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 42
BsaWI WCCGGW 2 cut(s) 26, 439
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 304, 424
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 1 cut(s) 358
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 3 cut(s) 92, 305, 419
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 3 cut(s) 259, 294, 507
BseLI CCNNNNNNNGG 3 cut(s) 165, 304, 424
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 3 cut(s) 232, 256, 484
BsgI GTGCAG 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 4 cut(s) 27, 165, 440, 465
BslI CCNNNNNNNGG 3 cut(s) 165, 304, 424
BsmAI GTCTC 1 cut(s) 445
BsmI GAATGC 3 cut(s) 204, 333, 432
Bsp1286I GDGCHC 1 cut(s) 123
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 4 cut(s) 87, 228, 333, 414
BspACI CCGC 2 cut(s) 223, 497
BspCNI CTCAG 1 cut(s) 190
BspEI TCCGGA 1 cut(s) 439
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 2 cut(s) 82, 409
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 4 cut(s) 87, 228, 333, 414
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 92, 305, 419
BstDEI CTNAG 1 cut(s) 198
BstF5I GGATG 3 cut(s) 259, 294, 507
BstKTI GATC 4 cut(s) 90, 231, 336, 417
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 4 cut(s) 87, 228, 333, 414
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstNI CCWGG 3 cut(s) 92, 305, 419
BstSCI CCNGG 4 cut(s) 90, 164, 303, 417
BstV1I GCAGC 3 cut(s) 232, 256, 484
BstV2I GAAGAC 4 cut(s) 161, 176, 350, 365
BstX2I RGATCY 3 cut(s) 87, 333, 414
BstXI CCANNNNNNTGG 3 cut(s) 43, 131, 181
BstYI RGATCY 3 cut(s) 87, 333, 414
BsuI GTATCC 1 cut(s) 55
BtsCI GGATG 3 cut(s) 259, 294, 507
BveI ACCTGC 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 442
CsiI ACCWGGT 1 cut(s) 303
CviJI RGCY 3 cut(s) 57, 247, 475
CviKI_1 RGCY 3 cut(s) 57, 247, 475
DdeI CTNAG 1 cut(s) 198
DpnI GATC 4 cut(s) 89, 230, 335, 416
DpnII GATC 4 cut(s) 87, 228, 333, 414
DraIII CACNNNGTG 1 cut(s) 118
DriI GACNNNNNGTC 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 90, 303, 417
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaiI YATR 1 cut(s) 140
FblI GTMKAC 2 cut(s) 104, 521
Fnu4HI GCNGC 3 cut(s) 221, 245, 473
FokI GGATG 3 cut(s) 266, 281, 494
Fsp4HI GCNGC 3 cut(s) 221, 245, 473
GluI GCNGC 3 cut(s) 221, 245, 473
GsuI CTGGAG 1 cut(s) 497
HapII CCGG 4 cut(s) 27, 165, 440, 465
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 5 cut(s) 106, 290, 453, 490, 518
HpaII CCGG 4 cut(s) 27, 165, 440, 465
HphI GGTGA 2 cut(s) 31, 521
Hpy166II GTNNAC 2 cut(s) 105, 522
Hpy188I TCNGA 3 cut(s) 61, 199, 560
Hpy188III TCNNGA 3 cut(s) 151, 182, 440
Hpy8I GTNNAC 2 cut(s) 105, 522
HpyAV CCTTC 4 cut(s) 73, 480, 536, 548
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 5 cut(s) 4, 142, 236, 301, 331
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 368
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 4 cut(s) 87, 228, 333, 414
LmnI GCTCC 1 cut(s) 126
Lsp1109I GCAGC 3 cut(s) 232, 256, 484
LweI GCATC 1 cut(s) 9
MabI ACCWGGT 1 cut(s) 303
MaeII ACGT 1 cut(s) 368
MalI GATC 4 cut(s) 89, 230, 335, 416
MboI GATC 4 cut(s) 87, 228, 333, 414
MboII GAAGA 6 cut(s) 139, 166, 181, 305, 355, 370
MflI RGATCY 3 cut(s) 87, 333, 414
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 1 cut(s) 309
MlyI GAGTC 5 cut(s) 100, 299, 447, 484, 527
MmeI TCCRAC 2 cut(s) 148, 351
MnlI CCTC 8 cut(s) 193, 244, 252, 367, 421, 447, 472, 554
MroI TCCGGA 1 cut(s) 439
MspA1I CMGCKG 2 cut(s) 223, 475
MspI CCGG 4 cut(s) 27, 165, 440, 465
MspR9I CCNGG 4 cut(s) 92, 166, 305, 419
Mva1269I GAATGC 3 cut(s) 204, 333, 432
MvaI CCWGG 3 cut(s) 92, 305, 419
MwoI GCNNNNNNNGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 166
NdeII GATC 4 cut(s) 87, 228, 333, 414
PctI GAATGC 3 cut(s) 204, 333, 432
PfoI TCCNGGA 2 cut(s) 90, 417
PkrI GCNGC 3 cut(s) 222, 246, 474
PleI GAGTC 5 cut(s) 100, 298, 447, 484, 526
PpsI GAGTC 5 cut(s) 100, 298, 447, 484, 526
Psp1406I AACGTT 1 cut(s) 368
Psp6I CCWGG 3 cut(s) 90, 303, 417
PspGI CCWGG 3 cut(s) 90, 303, 417
PspPI GGNCC 1 cut(s) 442
PsuI RGATCY 3 cut(s) 87, 333, 414
PvuII CAGCTG 1 cut(s) 475
SalI GTCGAC 1 cut(s) 103
SatI GCNGC 3 cut(s) 221, 245, 473
Sau3AI GATC 4 cut(s) 87, 228, 333, 414
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 5 cut(s) 100, 299, 447, 484, 527
ScrFI CCNGG 4 cut(s) 92, 166, 305, 419
SduI GDGCHC 1 cut(s) 123
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 1 cut(s) 9
SinI GGWCC 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 309
SsiI CCGC 2 cut(s) 223, 497
StyD4I CCNGG 4 cut(s) 90, 164, 303, 417
StyI CCWWGG 1 cut(s) 42
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 104
TasI AATT 1 cut(s) 309
TseI GCWGC 3 cut(s) 220, 244, 472
TspGWI ACGGA 2 cut(s) 498, 536
VpaK11BI GGWCC 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 2 cut(s) 131, 301
XmiI GTMKAC 2 cut(s) 104, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.