AT5G54720

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020843
Physical Location & Seq
Reverse (-)
22231448 .. 22232949
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54720.1

Sequence Viewer

Length: 558 bp
ATGAATCTATACGGATCATATCTAGACGACATTGCAGACCAGATACCGGTCGACAGGAGTCGGAGTCAGATGATCAGAAAGTACTTAGGTAAACAACGGAAAAATTTAGATTTGATAGATGAAGTCGTAGAGGCCAGACTTATACGTCAGTTGGAAGCCAATCAATCGATTTTGGAGAAAACAGACAAGCATAAATACGACTACGAGGAATATGCGACGATTTTGCATAGGGAAGAGTACGAGGAATATGCGACGAAGATAATCGACCTCTGCCCTTCACTCGTTAGAGTTGCTAACGTCGACGGAAACACTCCTCTTCATTTAGCTGCCGAAATTGGAAACGAATTTATCCTCTGGAAAATGTTGAGGTGTGGAGAAGCAGACTGCAGGAAGATTAATAAGCAAGGACAAACAGCATTCATATTAGCATGCCTCAACAATCACGTCGCTGTTGCACTTACCCTCCTACAATATATGCGGTCTATGACGATGGTGGAACTTGATGCAGCCTTTTCAAGACATCAACCAGGTTTGAAATTATCATCGCATAAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2

Protein Analysis

185

Amino Acids

21.34

Weight (kDa)

6.31

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 95 - 158 3e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54720
rosa_multiflora Rmu_sc0003029.1_g000001
rosa_rugosa Rorug02G0359900
rosa_samantha Rh1BG219000 Rh1DG245400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 144
AccI GTMKAC 2 cut(s) 51, 300
AciI CCGC 1 cut(s) 478
AclWI GGATC 1 cut(s) 22
AcsI RAATTY 2 cut(s) 103, 344
AfaI GTAC 2 cut(s) 83, 239
AfiI CCNNNNNNNGG 1 cut(s) 46
AgeI ACCGGT 1 cut(s) 46
AgsI TTSAA 2 cut(s) 516, 535
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
AlwI GGATC 1 cut(s) 22
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 2 cut(s) 326, 506
ApoI RAATTY 2 cut(s) 103, 344
AseI ATTAAT 1 cut(s) 396
AsiGI ACCGGT 1 cut(s) 46
BbvI GCAGC 2 cut(s) 313, 518
BccI CCATC 1 cut(s) 484
BcgI CGANNNNNNTGC 6 cut(s) 194, 205, 228, 230, 239, 264
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 1 cut(s) 72
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 385
BisI GCNGC 2 cut(s) 327, 507
BlsI GCNGC 2 cut(s) 328, 508
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 1 cut(s) 528
BmgBI CACGTC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 493
BoxI GACNNNNGTC 1 cut(s) 57
Bsa29I ATCGAT 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 447, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 30
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 2 cut(s) 313, 518
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 134
BshTI ACCGGT 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 1 cut(s) 47
BslI CCNNNNNNNGG 1 cut(s) 46
BsmI GAATGC 1 cut(s) 416
BsnI GGCC 1 cut(s) 134
Bsp143I GATC 2 cut(s) 14, 72
BspACI CCGC 1 cut(s) 478
BspANI GGCC 1 cut(s) 134
BspDI ATCGAT 1 cut(s) 167
BspMAI CTGCAG 1 cut(s) 389
BspPI GGATC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 46
BssMI GATC 2 cut(s) 14, 72
Bst2UI CCWGG 1 cut(s) 528
Bst6I CTCTTC 2 cut(s) 228, 321
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 1 cut(s) 85
BstKTI GATC 2 cut(s) 17, 75
BstMBI GATC 2 cut(s) 14, 72
BstMCI CGRYCG 1 cut(s) 51
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 432
BstPAI GACNNNNGTC 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 313, 518
Bsu15I ATCGAT 1 cut(s) 167
BsuRI GGCC 1 cut(s) 134
BsuTUI ATCGAT 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 528
BtrI CACGTC 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 82, 238
CspAI ACCGGT 1 cut(s) 46
CviAII CATG 1 cut(s) 429
CviJI RGCY 4 cut(s) 134, 158, 326, 509
CviKI_1 RGCY 4 cut(s) 134, 158, 326, 509
CviQI GTAC 2 cut(s) 82, 238
DdeI CTNAG 1 cut(s) 85
DpnI GATC 2 cut(s) 16, 74
DpnII GATC 2 cut(s) 14, 72
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 228, 321
EarI CTCTTC 2 cut(s) 228, 321
EcoRII CCWGG 1 cut(s) 526
FaeI CATG 1 cut(s) 432
FatI CATG 1 cut(s) 428
FbaI TGATCA 1 cut(s) 72
FblI GTMKAC 2 cut(s) 51, 300
Fnu4HI GCNGC 2 cut(s) 327, 507
Fsp4HI GCNGC 2 cut(s) 327, 507
FspBI CTAG 1 cut(s) 23
GluI GCNGC 2 cut(s) 327, 507
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 47
Hin1II CATG 1 cut(s) 432
HincII GTYRAC 2 cut(s) 52, 301
HindII GTYRAC 2 cut(s) 52, 301
HinfI GANTC 3 cut(s) 4, 58, 64
HpaII CCGG 1 cut(s) 47
Hpy166II GTNNAC 3 cut(s) 52, 92, 301
Hpy188I TCNGA 3 cut(s) 63, 69, 77
Hpy188III TCNNGA 3 cut(s) 23, 355, 516
Hpy8I GTNNAC 3 cut(s) 52, 92, 301
Hpy99I CGWCG 5 cut(s) 220, 256, 302, 305, 449
HpyAV CCTTC 1 cut(s) 285
HpyCH4IV ACGT 3 cut(s) 145, 297, 444
HpyCH4V TGCA 5 cut(s) 35, 226, 387, 455, 506
HpyF3I CTNAG 1 cut(s) 85
HpySE526I ACGT 3 cut(s) 145, 297, 444
Hsp92II CATG 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 2 cut(s) 14, 72
LpnPI CCDG 8 cut(s) 40, 53, 60, 148, 340, 373, 513, 540
Lsp1109I GCAGC 2 cut(s) 313, 518
LweI GCATC 1 cut(s) 493
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 145, 297, 444
MalI GATC 2 cut(s) 16, 74
MboI GATC 2 cut(s) 14, 72
MboII GAAGA 4 cut(s) 245, 268, 308, 403
MluCI AATT 4 cut(s) 103, 333, 344, 536
MlyI GAGTC 2 cut(s) 67, 73
MmeI TCCRAC 2 cut(s) 41, 132
MnlI CCTC 9 cut(s) 124, 199, 235, 278, 324, 360, 362, 443, 473
MseI TTAA 1 cut(s) 396
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 416
MvaI CCWGG 1 cut(s) 528
NdeII GATC 2 cut(s) 14, 72
NlaIII CATG 1 cut(s) 432
NspI RCATGY 1 cut(s) 432
PaeI GCATGC 1 cut(s) 432
PctI GAATGC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 4
PinAI ACCGGT 1 cut(s) 46
PkrI GCNGC 2 cut(s) 328, 508
PleI GAGTC 2 cut(s) 66, 72
PpsI GAGTC 2 cut(s) 66, 72
PshAI GACNNNNGTC 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PstI CTGCAG 1 cut(s) 389
RsaI GTAC 2 cut(s) 83, 239
RsaNI GTAC 2 cut(s) 82, 238
SalI GTCGAC 2 cut(s) 50, 299
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 2 cut(s) 327, 507
Sau3AI GATC 2 cut(s) 14, 72
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 2 cut(s) 67, 73
ScrFI CCNGG 1 cut(s) 528
SetI ASST 8 cut(s) 91, 148, 270, 300, 328, 371, 447, 532
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 385
SgrDI CGTCGACG 1 cut(s) 299
SphI GCATGC 1 cut(s) 432
Sse9I AATT 4 cut(s) 103, 333, 344, 536
SsiI CCGC 1 cut(s) 478
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 526
TaiI ACGT 3 cut(s) 148, 300, 447
TaqI TCGA 4 cut(s) 51, 167, 264, 300
TasI AATT 4 cut(s) 103, 333, 344, 536
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TseI GCWGC 2 cut(s) 326, 506
TspDTI ATGAA 4 cut(s) 17, 135, 308, 409
TspGWI ACGGA 3 cut(s) 27, 112, 318
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 2 cut(s) 103, 344
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 432
XmiI GTMKAC 2 cut(s) 51, 300
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.