AT5G65350

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0021846
Physical Location & Seq
Reverse (-)
26119211 .. 26119825
615 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65350.1

Sequence Viewer

Length: 420 bp
ATGGCTCGTACCAAGCAGACTGCTAGAATATCCACCGGAGGAAAAGCTCCGAGGAAGCAATTAGCGCCAAAAGCGGCGAGACAATCTGCACCAGCAACGGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACTGTTGCGTTGAGAGATATTAGGAAATACCAAAAGAGTACCGAGATTTTGATCAGAAAGCTTCCGTTTCAGCGACTTGTTCGTGAGATCGCTCAAGATTTCAAAACGGATCTGAGGTTTCAGAGCAGTGCCGTCGCTGCTCTCCAGGAAGCTGCTGAGGCTTACCTTGTTGGGTTATTCGAAGATACAAACCTCTGTGCGATTCACGCAAAGAGGGTGACGATCATGCCTAAAGAGATCCAATTAGCGAGAAGGATTAGAGGTGAGAGAGCTCGAGGAGAGTGA

Protein Analysis

139

Amino Acids

15.59

Weight (kDa)

11.25

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 6.3e-37 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021846)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65350
pyrus_communis pycom11g02050
rosa_samantha Rh5BG533700 Rh5CG557300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AclWI GGATC 2 cut(s) 252, 367
AfaI GTAC 2 cut(s) 10, 175
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 2 cut(s) 130, 279
AluBI AGCT 4 cut(s) 47, 196, 287, 407
AluI AGCT 4 cut(s) 47, 196, 287, 407
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 2 cut(s) 252, 367
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 408
ApeKI GCWGC 2 cut(s) 272, 287
AspLEI GCGC 1 cut(s) 67
AsuHPI GGTGA 2 cut(s) 364, 410
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 408
BanII GRGCYC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 409
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 259, 274
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 2 cut(s) 132, 281
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 3 cut(s) 75, 273, 288
BlsI GCNGC 3 cut(s) 76, 274, 289
Bme1390I CCNGG 2 cut(s) 132, 281
BmeT110I CYCGRG 1 cut(s) 408
BmrFI CCNGG 2 cut(s) 132, 281
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse8I GATNNNNATC 1 cut(s) 185
BseBI CCWGG 2 cut(s) 132, 281
BseDI CCNNGG 1 cut(s) 50
BseJI GATNNNNATC 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 2 cut(s) 239, 282
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 2 cut(s) 259, 274
BsgI GTGCAG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 5 cut(s) 186, 222, 244, 357, 372
BspACI CCGC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 240, 283
BspPI GGATC 2 cut(s) 252, 367
BspT104I TTCGAA 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 5 cut(s) 186, 222, 244, 357, 372
Bst2UI CCWGG 2 cut(s) 132, 281
Bst4CI ACNGT 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 315
BstDEI CTNAG 2 cut(s) 248, 291
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 5 cut(s) 189, 225, 247, 360, 375
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 5 cut(s) 186, 222, 244, 357, 372
BstMWI GCNNNNNNNGC 5 cut(s) 64, 71, 272, 293, 341
BstNI CCWGG 2 cut(s) 132, 281
BstSCI CCNGG 2 cut(s) 130, 279
BstV1I GCAGC 2 cut(s) 259, 274
BstX2I RGATCY 2 cut(s) 244, 372
BstYI RGATCY 2 cut(s) 244, 372
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 67
Csp6I GTAC 2 cut(s) 9, 174
CviAII CATG 1 cut(s) 361
CviJI RGCY 7 cut(s) 5, 47, 115, 196, 287, 296, 407
CviKI_1 RGCY 7 cut(s) 5, 47, 115, 196, 287, 296, 407
CviQI GTAC 2 cut(s) 9, 174
DdeI CTNAG 2 cut(s) 248, 291
DpnI GATC 5 cut(s) 188, 224, 246, 359, 374
DpnII GATC 5 cut(s) 186, 222, 244, 357, 372
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 407
EcoRII CCWGG 2 cut(s) 130, 279
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 1 cut(s) 364
FaiI YATR 1 cut(s) 362
FatI CATG 1 cut(s) 360
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 75, 273, 288
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 3 cut(s) 75, 273, 288
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 75, 273, 288
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 68
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 67
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 123, 337
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 364, 410
Hpy188I TCNGA 4 cut(s) 51, 191, 249, 258
Hpy188III TCNNGA 2 cut(s) 218, 230
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 1 cut(s) 381
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 71, 272, 293, 341
HpyF3I CTNAG 2 cut(s) 248, 291
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 5 cut(s) 186, 222, 244, 357, 372
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 6 cut(s) 49, 105, 117, 144, 266, 293
Lsp1109I GCAGC 2 cut(s) 259, 274
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 352
MalI GATC 5 cut(s) 188, 224, 246, 359, 374
MboI GATC 5 cut(s) 186, 222, 244, 357, 372
MboII GAAGA 2 cut(s) 121, 329
MflI RGATCY 2 cut(s) 244, 372
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 2 cut(s) 59, 377
MnlI CCTC 9 cut(s) 32, 45, 95, 243, 286, 338, 342, 389, 404
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 132, 281
MvaI CCWGG 2 cut(s) 132, 281
MwoI GCNNNNNNNGC 5 cut(s) 64, 71, 272, 293, 341
NdeII GATC 5 cut(s) 186, 222, 244, 357, 372
NlaIII CATG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 408
PfeI GAWTC 2 cut(s) 123, 337
PfoI TCCNGGA 2 cut(s) 130, 279
PkrI GCNGC 3 cut(s) 76, 274, 289
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 130, 279
PspGI CCWGG 2 cut(s) 130, 279
PspXI VCTCGAGB 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 244, 372
RsaI GTAC 2 cut(s) 10, 175
RsaNI GTAC 2 cut(s) 9, 174
SacI GAGCTC 1 cut(s) 409
SatI GCNGC 3 cut(s) 75, 273, 288
Sau3AI GATC 5 cut(s) 186, 222, 244, 357, 372
ScrFI CCNGG 2 cut(s) 132, 281
SduI GDGCHC 1 cut(s) 409
SetI ASST 8 cut(s) 49, 198, 254, 289, 303, 330, 400, 409
Sfr274I CTCGAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 228, 408
SmoI CTYRAG 2 cut(s) 228, 408
Sse9I AATT 2 cut(s) 59, 377
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 2 cut(s) 130, 279
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 315, 409
TasI AATT 2 cut(s) 59, 377
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 2 cut(s) 123, 337
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 272, 287
Tsp45I GTSAC 1 cut(s) 352
TspGWI ACGGA 3 cut(s) 116, 189, 257
TspRI CASTG 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 408
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.