Rh5BG533700

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0021846
Physical Location & Seq
Reverse (-)
84164491 .. 84164901
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG533700.1

Sequence Viewer

Length: 411 bp
ATGGCTCGTACCAAGCAGACCGCTCGCAAGTCCACCGGAGGAAAGGCGCCTAGGAAACAGCTGGCGACCAAGGCGGCCCGGAAGTCGGCTCCGGCGACCGGAGGAGTGAAGAAGCCTCACCGGTTCAGGCCGGGAACGGTGGCGCTGAGAGAGATCAGGAAGTACCAGAAGAGCACGGAGCTTCTGATCCGCAAGCTTCCGTTCCAGAGGCTTGTGAGGGAGATTGCTCAGGATTTCAAGACGGATCTCCGATTCCAGAGCAGCGCCGTGACGGCGCTTCAGGAGGCGGCGGAGGCGTACCTGGTGGGGCTTTTCGAAGACACAAATCTCTGCGCTATTCATGCTAAGAGGGTTACTATAATGCCCAAGGATATTCAGCTTGCGAGGAGAATCAGAGGCGAGAGGGCTTAG

Protein Analysis

136

Amino Acids

15.3

Weight (kDa)

11.29

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 6.3e-39 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021846)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65350
pyrus_communis pycom11g02050
rosa_samantha Rh5BG533700 Rh5CG557300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 23
AciI CCGC 5 cut(s) 21, 74, 190, 287, 290
AclWI GGATC 2 cut(s) 181, 252
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 3 cut(s) 10, 164, 299
AfiI CCNNNNNNNGG 2 cut(s) 85, 98
AgeI ACCGGT 1 cut(s) 120
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 4 cut(s) 61, 181, 196, 379
AluI AGCT 4 cut(s) 61, 181, 196, 379
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 2 cut(s) 181, 252
AoxI GGCC 2 cut(s) 75, 128
ApeKI GCWGC 1 cut(s) 261
AsiGI ACCGGT 1 cut(s) 120
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 5 cut(s) 49, 145, 266, 277, 335
AspS9I GGNCC 1 cut(s) 76
AsuC2I CCSGG 2 cut(s) 79, 132
AsuHPI GGTGA 1 cut(s) 110
AsuII TTCGAA 1 cut(s) 315
AvrII CCTAGG 1 cut(s) 50
BanI GGYRCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 273
BceAI ACGGC 2 cut(s) 251, 288
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 302
BcnI CCSGG 2 cut(s) 79, 132
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 4 cut(s) 50, 146, 267, 278
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 3 cut(s) 75, 262, 288
BlnI CCTAGG 1 cut(s) 50
BlsI GCNGC 3 cut(s) 76, 263, 289
Bme1390I CCNGG 3 cut(s) 79, 132, 302
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 2 cut(s) 48, 90
BmrFI CCNGG 3 cut(s) 79, 132, 302
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 315
BpuMI CCSGG 2 cut(s) 79, 132
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 50, 69, 366
BsaWI WCCGGW 3 cut(s) 35, 98, 120
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 98
Bse118I RCCGGY 1 cut(s) 120
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 50, 69, 366
BseLI CCNNNNNNNGG 2 cut(s) 85, 98
BseMII CTCAG 2 cut(s) 137, 242
BseRI GAGGAG 2 cut(s) 117, 400
BseXI GCAGC 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 77, 130
BshNI GGYRCC 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 6 cut(s) 36, 79, 92, 99, 121, 131
BslI CCNNNNNNNGG 2 cut(s) 85, 98
BsnI GGCC 2 cut(s) 77, 130
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 3 cut(s) 153, 186, 244
BspACI CCGC 5 cut(s) 21, 74, 190, 287, 290
BspANI GGCC 2 cut(s) 77, 130
BspCNI CTCAG 2 cut(s) 138, 241
BspLI GGNNCC 2 cut(s) 48, 90
BspPI GGATC 2 cut(s) 181, 252
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 3 cut(s) 50, 69, 366
BssMI GATC 3 cut(s) 153, 186, 244
BssNI GRCGYC 1 cut(s) 47
BssT1I CCWWGG 3 cut(s) 50, 69, 366
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 4 cut(s) 25, 63, 194, 381
BstDEI CTNAG 4 cut(s) 146, 228, 345, 408
BstH2I RGCGCY 4 cut(s) 50, 146, 267, 278
BstHHI GCGC 5 cut(s) 49, 145, 266, 277, 335
BstKTI GATC 3 cut(s) 156, 189, 247
BstMBI GATC 3 cut(s) 153, 186, 244
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 77, 130, 300
BstV1I GCAGC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 77, 130
Cac8I GCNNGC 4 cut(s) 25, 63, 194, 381
CfoI GCGC 5 cut(s) 49, 145, 266, 277, 335
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 76
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 3 cut(s) 9, 163, 298
CspAI ACCGGT 1 cut(s) 120
CviAII CATG 1 cut(s) 341
CviQI GTAC 3 cut(s) 9, 163, 298
DdeI CTNAG 4 cut(s) 146, 228, 345, 408
DinI GGCGCC 1 cut(s) 48
DpnI GATC 3 cut(s) 155, 188, 246
DpnII GATC 3 cut(s) 153, 186, 244
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 3 cut(s) 50, 69, 366
Eco57I CTGAAG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 3 cut(s) 50, 69, 366
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 50, 69, 366
FaeI CATG 1 cut(s) 344
FaiI YATR 2 cut(s) 342, 359
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 3 cut(s) 75, 262, 288
Fsp4HI GCNGC 3 cut(s) 75, 262, 288
FspBI CTAG 1 cut(s) 51
GlaI GCGC 5 cut(s) 48, 144, 265, 276, 334
GluI GCNGC 3 cut(s) 75, 262, 288
HaeII RGCGCY 4 cut(s) 50, 146, 267, 278
HaeIII GGCC 2 cut(s) 77, 130
HapII CCGG 6 cut(s) 36, 79, 92, 99, 121, 131
HhaI GCGC 5 cut(s) 49, 145, 266, 277, 335
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 5 cut(s) 47, 143, 264, 275, 333
HinP1I GCGC 5 cut(s) 47, 143, 264, 275, 333
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 252, 390
HpaII CCGG 6 cut(s) 36, 79, 92, 99, 121, 131
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 186, 251, 395
Hpy188III TCNNGA 6 cut(s) 157, 205, 230, 238, 256, 281
Hpy8I GTNNAC 1 cut(s) 33
HpyCH4III ACNGT 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 4 cut(s) 146, 228, 345, 408
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 5 cut(s) 47, 143, 264, 275, 333
KasI GGCGCC 1 cut(s) 46
Kzo9I GATC 3 cut(s) 153, 186, 244
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 94, 178
Lsp1109I GCAGC 1 cut(s) 273
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 268, 352
MalI GATC 3 cut(s) 155, 188, 246
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 153, 186, 244
MboII GAAGA 3 cut(s) 121, 181, 329
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 6 cut(s) 36, 79, 92, 99, 121, 131
MspR9I CCNGG 3 cut(s) 79, 132, 302
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NarI GGCGCC 1 cut(s) 47
NciI CCSGG 2 cut(s) 79, 132
NdeII GATC 3 cut(s) 153, 186, 244
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 48, 90
NmuCI GTSAC 1 cut(s) 268
NspV TTCGAA 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 2 cut(s) 252, 390
PinAI ACCGGT 1 cut(s) 120
PkrI GCNGC 3 cut(s) 76, 263, 289
PluTI GGCGCC 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 48, 90
PspPI GGNCC 1 cut(s) 76
PsuI RGATCY 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 3 cut(s) 10, 164, 299
RsaNI GTAC 3 cut(s) 9, 163, 298
SapI GCTCTTC 1 cut(s) 164
SatI GCNGC 3 cut(s) 75, 262, 288
Sau3AI GATC 3 cut(s) 153, 186, 244
Sau96I GGNCC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 79, 132, 302
SduI GDGCHC 1 cut(s) 176
SetI ASST 5 cut(s) 63, 183, 198, 303, 381
SexAI ACCWGGT 1 cut(s) 300
SfoI GGCGCC 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 315
SsiI CCGC 5 cut(s) 21, 74, 190, 287, 290
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 77, 130, 300
StyI CCWWGG 3 cut(s) 50, 69, 366
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 315
TauI GCSGC 2 cut(s) 77, 290
TfiI GAWTC 2 cut(s) 252, 390
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 261
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 3 cut(s) 189, 191, 257
XmaJI CCTAGG 1 cut(s) 50
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.