ATCG00720

cytochrome b6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0023598
Physical Location & Seq
Forward (+)
74841 .. 76292
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
ATCG00720.1

Sequence Viewer

Length: 648 bp
ATGAGTAAAGTTTATGATTGGTTCGAAGAACGTCTTGAGATTCAGGCGATTGCAGATGATATAACTAGTAAATATGTTCCTCCGCATGTCAACATATTTTATTGTCTAGGCGGAATTACCCTTACTTGTTTTTTAGTACAAGTAGCTACGGGATTTGCTATGACTTTTTATTACCGTCCAACCGTTACTGAAGCTTTTGCTTCTGTTCAATATATAATGACTGAAGCTAACTTTGGTTGGTTAATCCGATCAGTTCATCGATGGTCGGCAAGTATGATGGTCCTAATGATGATCCTGCACGTATTTCGTGTATACCTCACCGGTGGTTTTAAAAAACCTCGCGAATTAACTTGGGTTACTGGTGTGGTTCTGGGTGTATTGACCGCATCTTTTGGTGTAACAGGTTATTCTTTACCTTGGGATCAAATTGGTTATTGGGCGGTCAAAATTGTAACAGGTGTACCTGACGCTATTCCGGTAATAGGATCACCTCTTGTAGAATTATTACGCGGAAGTGCTAGTGTTGGACAATCCACTTTGACTCGTTTTTATAGTTTACACACTTTTGTATTACCTCTTCTTACGGCCGTCTTTATGTTAATGCATTTCCTAATGATACGTAAGCAAGGTATTTCTGGTCCCTTATAA

Protein Analysis

215

Amino Acids

24.15

Weight (kDa)

8.89

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_B PF00033 22 - 210 2.3e-86 Cytochrome b/b6/petB
Cytochrom_B_N_2 PF13631 92 - 211 6.9e-38 Cytochrome b(N-terminal)/b6/petB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023598)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00720
malus_domestica MD11G1138700.v1.1
prunus_persica Prupe.1G203800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 646
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 342, 510
AciI CCGC 5 cut(s) 83, 111, 384, 440, 510
AclWI GGATC 3 cut(s) 286, 429, 493
AcoI YGGCCR 1 cut(s) 585
AcuI CTGAAG 2 cut(s) 210, 243
AfaI GTAC 2 cut(s) 138, 462
AfiI CCNNNNNNNGG 1 cut(s) 482
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 1 cut(s) 209
AhlI ACTAGT 1 cut(s) 65
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 3 cut(s) 146, 194, 227
AluI AGCT 3 cut(s) 146, 194, 227
AlwI GGATC 3 cut(s) 286, 429, 493
AoxI GGCC 1 cut(s) 585
AsiGI ACCGGT 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 280, 638
AsuHPI GGTGA 2 cut(s) 310, 480
AsuII TTCGAA 1 cut(s) 24
AvaII GGWCC 2 cut(s) 280, 638
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
BccI CCATC 2 cut(s) 255, 271
BceAI ACGGC 2 cut(s) 572, 600
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 3 cut(s) 66, 107, 519
Bme18I GGWCC 2 cut(s) 280, 638
BmgT120I GGNCC 2 cut(s) 280, 638
BmiI GGNNCC 1 cut(s) 640
BmsI GCATC 1 cut(s) 395
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 259
BsaAI YACGTR 2 cut(s) 301, 620
BsaJI CCNNGG 1 cut(s) 416
BsaWI WCCGGW 2 cut(s) 320, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 364
BseCI ATCGAT 1 cut(s) 259
BseDI CCNNGG 1 cut(s) 416
BseLI CCNNNNNNNGG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 364
BseX3I CGGCCG 1 cut(s) 585
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 2 cut(s) 342, 510
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 1 cut(s) 587
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 588
BsiSI CCGG 2 cut(s) 321, 476
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 1 cut(s) 587
Bsp119I TTCGAA 1 cut(s) 24
Bsp143I GATC 4 cut(s) 248, 291, 421, 485
Bsp68I TCGCGA 1 cut(s) 342
BspACI CCGC 5 cut(s) 83, 111, 384, 440, 510
BspANI GGCC 1 cut(s) 587
BspDI ATCGAT 1 cut(s) 259
BspFNI CGCG 2 cut(s) 342, 510
BspLI GGNNCC 1 cut(s) 640
BspPI GGATC 3 cut(s) 286, 429, 493
BspT104I TTCGAA 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 364
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 4 cut(s) 248, 291, 421, 485
BssNAI GTATAC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 416
Bst1107I GTATAC 1 cut(s) 313
Bst4CI ACNGT 2 cut(s) 176, 184
Bst6I CTCTTC 1 cut(s) 582
BstBAI YACGTR 2 cut(s) 301, 620
BstBI TTCGAA 1 cut(s) 24
BstFNI CGCG 2 cut(s) 342, 510
BstKTI GATC 4 cut(s) 251, 294, 424, 488
BstMBI GATC 4 cut(s) 248, 291, 421, 485
BstMCI CGRYCG 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 89
BstSNI TACGTA 1 cut(s) 620
BstUI CGCG 2 cut(s) 342, 510
BstZ17I GTATAC 1 cut(s) 313
BstZI CGGCCG 1 cut(s) 585
Bsu15I ATCGAT 1 cut(s) 259
BsuRI GGCC 1 cut(s) 587
BsuTUI ATCGAT 1 cut(s) 259
BtuMI TCGCGA 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 280, 638
ClaI ATCGAT 1 cut(s) 259
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 137, 461
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 1 cut(s) 86
CviJI RGCY 4 cut(s) 146, 194, 227, 587
CviKI_1 RGCY 4 cut(s) 146, 194, 227, 587
CviQI GTAC 2 cut(s) 137, 461
DpnI GATC 4 cut(s) 250, 293, 423, 487
DpnII GATC 4 cut(s) 248, 291, 421, 485
DraI TTTAAA 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 585
EagI CGGCCG 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 126
EclXI CGGCCG 1 cut(s) 585
Eco105I TACGTA 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 280, 638
Eco52I CGGCCG 1 cut(s) 585
Eco57I CTGAAG 2 cut(s) 210, 243
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 1 cut(s) 89
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 1 cut(s) 624
FatI CATG 1 cut(s) 85
FblI GTMKAC 1 cut(s) 312
FspBI CTAG 3 cut(s) 66, 107, 519
HaeIII GGCC 1 cut(s) 587
HapII CCGG 2 cut(s) 321, 476
HgaI GACGC 1 cut(s) 476
Hin1II CATG 1 cut(s) 89
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 40, 541
HpaII CCGG 2 cut(s) 321, 476
HphI GGTGA 2 cut(s) 310, 480
Hpy166II GTNNAC 4 cut(s) 91, 313, 461, 557
Hpy188I TCNGA 1 cut(s) 248
Hpy188III TCNNGA 2 cut(s) 35, 341
Hpy8I GTNNAC 4 cut(s) 91, 313, 461, 557
HpyCH4III ACNGT 2 cut(s) 176, 184
HpyCH4IV ACGT 3 cut(s) 31, 300, 619
HpyCH4V TGCA 3 cut(s) 53, 298, 604
HpySE526I ACGT 3 cut(s) 31, 300, 619
Hsp92II CATG 1 cut(s) 89
Kzo9I GATC 4 cut(s) 248, 291, 421, 485
LweI GCATC 1 cut(s) 395
MaeI CTAG 3 cut(s) 66, 107, 519
MaeII ACGT 3 cut(s) 31, 300, 619
MaeIII GTNAC 4 cut(s) 184, 355, 397, 451
MalI GATC 4 cut(s) 250, 293, 423, 487
MboI GATC 4 cut(s) 248, 291, 421, 485
MboII GAAGA 2 cut(s) 38, 569
MluCI AATT 5 cut(s) 114, 344, 426, 447, 500
MlyI GAGTC 1 cut(s) 535
MmeI TCCRAC 2 cut(s) 203, 505
MnlI CCTC 5 cut(s) 90, 326, 348, 501, 585
Mph1103I ATGCAT 1 cut(s) 606
MseI TTAA 4 cut(s) 242, 330, 347, 599
MspI CCGG 2 cut(s) 321, 476
MvnI CGCG 2 cut(s) 342, 510
NdeII GATC 4 cut(s) 248, 291, 421, 485
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 640
NruI TCGCGA 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 606
NspI RCATGY 1 cut(s) 89
NspV TTCGAA 1 cut(s) 24
PfeI GAWTC 1 cut(s) 40
PinAI ACCGGT 1 cut(s) 320
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
Ppu21I YACGTR 2 cut(s) 301, 620
PsiI TTATAA 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 640
PspPI GGNCC 2 cut(s) 280, 638
RruI TCGCGA 1 cut(s) 342
RsaI GTAC 2 cut(s) 138, 462
RsaNI GTAC 2 cut(s) 137, 461
SaqAI TTAA 4 cut(s) 242, 330, 347, 599
Sau3AI GATC 4 cut(s) 248, 291, 421, 485
Sau96I GGNCC 2 cut(s) 280, 638
SchI GAGTC 1 cut(s) 535
SfaNI GCATC 1 cut(s) 395
SfuI TTCGAA 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 2 cut(s) 280, 638
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
SnaBI TACGTA 1 cut(s) 620
SpeI ACTAGT 1 cut(s) 65
Sse9I AATT 5 cut(s) 114, 344, 426, 447, 500
SsiI CCGC 5 cut(s) 83, 111, 384, 440, 510
SspMI CTAG 3 cut(s) 66, 107, 519
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 2 cut(s) 176, 184
TaiI ACGT 3 cut(s) 34, 303, 622
TaqI TCGA 2 cut(s) 24, 259
TasI AATT 5 cut(s) 114, 344, 426, 447, 500
TatI WGTACW 1 cut(s) 136
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 4 cut(s) 242, 330, 347, 599
Tru9I TTAA 4 cut(s) 242, 330, 347, 599
TspDTI ATGAA 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 280, 638
XceI RCATGY 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 3 cut(s) 66, 107, 519
Zsp2I ATGCAT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.