Prupe.1G203800_v2.0.a1

Cytochrome b(N-terminal)/b6/petB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0023598
Physical Location & Seq
Reverse (-)
20333800 .. 20334637
838 bp
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UTR
Exon/CDS
Intron
Prupe.1G203800.1

Sequence Viewer

Length: 618 bp
CCTCTTGAGATTCAGGCAATTGCCGATGATATAACTAGTAAATATGTTACTCCTCACGTCAACATATTTTATTGTCTAGGAGGAATTACGCTTACTTGTTTTTTAGTACAAGTAGCTATAGGGTTTGCTATGACTTTTTATTATCGTTCGACCGTTACAGAGACTTTTGCTGCTGTTCAATATATGATGACTAAAGCTAATTTTGGTTGGTTAATCCGATCGGTTCATCGATGGTCGACAAGTATGATGGTCCTAATAATGATCCTGCACGTATTTCGTGTGTATCTAACCGGTGGCTTTAAAAAACCTCGCGAATTGACTTGGGTTACAGGTGTGGTTTTGGGTGTATTGACCGCATCTTTTGGTGTAACTGGTTATTCCTTACCTCAGGACCAAATTGGTTATTGGGCAGTAAAAATTGTAACAGGCATACCAGACGCTATTCCTGTAATAGGCTCGCCCCTGGTAGAGTTATTACGCGGAAGTGCTAGTGTGGGACAATCCACTTTGACTCGTTTTTATAGTTTACACACTTTTGTATTACCTCTTCTTACAGCCGTATTTATGTTAATGCACTTCCCAATGATACGTAAGCAAGGTATTTCTGGTCCTTTATAA

Protein Analysis

206

Amino Acids

22.77

Weight (kDa)

9.49

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023598)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00720
malus_domestica MD11G1138700.v1.1
prunus_persica Prupe.1G203800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 616
AccI GTMKAC 1 cut(s) 236
AccII CGCG 2 cut(s) 312, 480
AciI CCGC 2 cut(s) 354, 480
AclWI GGATC 1 cut(s) 256
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 452
AgeI ACCGGT 1 cut(s) 290
AgsI TTSAA 1 cut(s) 179
AhlI ACTAGT 1 cut(s) 35
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 2 cut(s) 116, 197
AluI AGCT 2 cut(s) 116, 197
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 256
ApeKI GCWGC 1 cut(s) 170
AsiGI ACCGGT 1 cut(s) 290
AspS9I GGNCC 3 cut(s) 250, 391, 608
AvaII GGWCC 3 cut(s) 250, 391, 608
AxyI CCTNAGG 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 531, 563
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 225, 241
BceAI ACGGC 1 cut(s) 542
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 155
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 3 cut(s) 36, 77, 489
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 3 cut(s) 250, 391, 608
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 250, 391, 608
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 229
BsaAI YACGTR 2 cut(s) 271, 590
BsaJI CCNNGG 1 cut(s) 462
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 376
Bse21I CCTNAGG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 251
Bsh1236I CGCG 2 cut(s) 312, 480
Bsh1285I CGRYCG 2 cut(s) 153, 221
BshTI ACCGGT 1 cut(s) 290
BshVI ATCGAT 1 cut(s) 229
BsiEI CGRYCG 2 cut(s) 153, 221
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 510
Bsp143I GATC 2 cut(s) 218, 261
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 2 cut(s) 354, 480
BspCNI CTCAG 1 cut(s) 400
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 2 cut(s) 312, 480
BspPI GGATC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 376
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 2 cut(s) 218, 261
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 552
BstBAI YACGTR 2 cut(s) 271, 590
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 1 cut(s) 387
BstENI CCTNNNNNAGG 1 cut(s) 450
BstFNI CGCG 2 cut(s) 312, 480
BstKTI GATC 2 cut(s) 221, 264
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 218, 261
BstMCI CGRYCG 2 cut(s) 153, 221
BstNI CCWGG 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 1 cut(s) 117
BstSNI TACGTA 1 cut(s) 590
BstUI CGCG 2 cut(s) 312, 480
BstV1I GCAGC 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 229
BtrI CACGTC 1 cut(s) 58
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 3 cut(s) 250, 391, 608
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 1 cut(s) 446
Csp6I GTAC 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 290
CviJI RGCY 5 cut(s) 116, 197, 297, 456, 557
CviKI_1 RGCY 5 cut(s) 116, 197, 297, 456, 557
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 220, 263
DpnII GATC 2 cut(s) 218, 261
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 590
Eco47I GGWCC 3 cut(s) 250, 391, 608
Eco81I CCTNAGG 1 cut(s) 387
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoRII CCWGG 1 cut(s) 462
FaqI GGGAC 1 cut(s) 510
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 3 cut(s) 36, 77, 489
GluI GCNGC 1 cut(s) 171
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 446
HincII GTYRAC 2 cut(s) 61, 237
HindII GTYRAC 2 cut(s) 61, 237
HinfI GANTC 2 cut(s) 10, 511
HpaII CCGG 1 cut(s) 291
Hpy166II GTNNAC 3 cut(s) 61, 237, 527
Hpy188I TCNGA 1 cut(s) 218
Hpy188III TCNNGA 3 cut(s) 5, 311, 389
Hpy8I GTNNAC 3 cut(s) 61, 237, 527
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 3 cut(s) 57, 270, 589
HpyCH4V TGCA 2 cut(s) 268, 574
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 3 cut(s) 57, 270, 589
Kzo9I GATC 2 cut(s) 218, 261
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 365
MaeI CTAG 3 cut(s) 36, 77, 489
MaeII ACGT 3 cut(s) 57, 270, 589
MaeIII GTNAC 5 cut(s) 46, 154, 325, 367, 421
MalI GATC 2 cut(s) 220, 263
MboI GATC 2 cut(s) 218, 261
MboII GAAGA 1 cut(s) 539
MfeI CAATTG 1 cut(s) 18
MluCI AATT 6 cut(s) 18, 84, 199, 314, 396, 417
MlyI GAGTC 1 cut(s) 505
MnlI CCTC 6 cut(s) 12, 63, 74, 318, 396, 555
MseI TTAA 3 cut(s) 212, 300, 569
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 464
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 2 cut(s) 312, 480
NdeII GATC 2 cut(s) 218, 261
NruI TCGCGA 1 cut(s) 312
PfeI GAWTC 1 cut(s) 10
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 1 cut(s) 172
Ple19I CGATCG 1 cut(s) 221
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Ppu21I YACGTR 2 cut(s) 271, 590
PsiI TTATAA 1 cut(s) 616
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspPI GGNCC 3 cut(s) 250, 391, 608
PvuI CGATCG 1 cut(s) 221
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SalI GTCGAC 1 cut(s) 235
SaqAI TTAA 3 cut(s) 212, 300, 569
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 218, 261
Sau96I GGNCC 3 cut(s) 250, 391, 608
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 1 cut(s) 464
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 3 cut(s) 250, 391, 608
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
SnaBI TACGTA 1 cut(s) 590
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 6 cut(s) 18, 84, 199, 314, 396, 417
SsiI CCGC 2 cut(s) 354, 480
SspMI CTAG 3 cut(s) 36, 77, 489
StyD4I CCNGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 3 cut(s) 60, 273, 592
TaqI TCGA 3 cut(s) 149, 229, 236
TasI AATT 6 cut(s) 18, 84, 199, 314, 396, 417
TatI WGTACW 1 cut(s) 106
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 3 cut(s) 212, 300, 569
Tru9I TTAA 3 cut(s) 212, 300, 569
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 215
VpaK11BI GGWCC 3 cut(s) 250, 391, 608
XagI CCTNNNNNAGG 1 cut(s) 450
XmiI GTMKAC 1 cut(s) 236
XspI CTAG 3 cut(s) 36, 77, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.