ATMG01275

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Mt
OG0020171
Physical Location & Seq
Reverse (-)
318004 .. 318390
387 bp
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UTR
Exon/CDS
Intron
ATMG01275.1

Sequence Viewer

Length: 387 bp
ACGTACATAGCTGTTCCAGCTGAAATACTTGGAATAATTCTACCACTTCTACTAGGAGTAGCCTTTTTAGTGCTAGCTGAACGTAAAGTAATGGCTTTTGTGCAACGTAGAAAGGGTCCTGATGTAGTGGGATCGTTCGGATTGTTACAACCTCTAGCAGATGGTTCGAAATTGATTCTAAAAGAACCTATTTCACCAAGTAGTGCTAATTTTTTCCTTTTTAGAATGGCTCCAGTGGCTACATTTATGTTAAGTCTGGTCGCTCGGGCCGTTGTACCTTTTGATTATGGTATGGTATTGTCAGATCCGAACATAGGGCTACTTTATTTGTTTGCCATATCTTCGCTAGGTGTTTATGGAATTATTATAGCGGGTCGGTCTAGTAAT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006091 GO:0008150 GO:0008152 GO:0009987 GO:0015980 GO:0044237 GO:0045333 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
NADHdh

Protein Analysis

129

Amino Acids

13.8

Weight (kDa)

9.52

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADHdh PF00146 8 - 129 6.6e-39 NADH dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07785 ATMG01275
rosa_chinensis RchiOBHm_Chr6g0256751 RchiOBHm_MTg0498831
rosa_roxburghii Rroxscaffold_2G00119320
rosa_samantha Rh6CG178200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 371
AclWI GGATC 2 cut(s) 139, 299
AfaI GTAC 2 cut(s) 5, 276
AfiI CCNNNNNNNGG 1 cut(s) 314
AluBI AGCT 3 cut(s) 11, 20, 77
AluI AGCT 3 cut(s) 11, 20, 77
AlwI GGATC 2 cut(s) 139, 299
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 116, 267
AsuHPI GGTGA 1 cut(s) 186
AsuII TTCGAA 1 cut(s) 167
AsuNHI GCTAGC 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 116
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BfaI CTAG 5 cut(s) 53, 74, 155, 347, 381
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 2 cut(s) 116, 267
BmiI GGNNCC 2 cut(s) 117, 231
BmtI GCTAGC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 233
BseLI CCNNNNNNNGG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 233
BshFI GGCC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 314
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 167
Bsp143I GATC 2 cut(s) 131, 304
BspACI CCGC 1 cut(s) 371
BspANI GGCC 1 cut(s) 269
BspLI GGNNCC 2 cut(s) 117, 231
BspOI GCTAGC 1 cut(s) 77
BspPI GGATC 2 cut(s) 139, 299
BspT104I TTCGAA 1 cut(s) 167
BsrI ACTGG 1 cut(s) 233
BssMI GATC 2 cut(s) 131, 304
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 134, 307
BstMBI GATC 2 cut(s) 131, 304
BstMWI GCNNNNNNNGC 2 cut(s) 17, 236
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 1 cut(s) 269
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 116, 267
Csp6I GTAC 2 cut(s) 4, 275
CviJI RGCY 9 cut(s) 11, 20, 62, 77, 95, 230, 239, 269, 319
CviKI_1 RGCY 9 cut(s) 11, 20, 62, 77, 95, 230, 239, 269, 319
CviQI GTAC 2 cut(s) 4, 275
DpnI GATC 2 cut(s) 133, 306
DpnII GATC 2 cut(s) 131, 304
Eco47I GGWCC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 116
FaiI YATR 8 cut(s) 8, 248, 288, 293, 314, 338, 357, 368
FauI CCCGC 1 cut(s) 364
FspBI CTAG 5 cut(s) 53, 74, 155, 347, 381
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 269
HinfI GANTC 1 cut(s) 175
HphI GGTGA 1 cut(s) 186
Hpy188I TCNGA 3 cut(s) 140, 304, 309
Hpy188III TCNNGA 1 cut(s) 119
HpyCH4IV ACGT 3 cut(s) 2, 82, 106
HpyCH4V TGCA 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 236
HpySE526I ACGT 3 cut(s) 2, 82, 106
Kzo9I GATC 2 cut(s) 131, 304
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 4 cut(s) 30, 132, 242, 246
MaeI CTAG 5 cut(s) 53, 74, 155, 347, 381
MaeII ACGT 3 cut(s) 2, 82, 106
MaeIII GTNAC 1 cut(s) 144
MalI GATC 2 cut(s) 133, 306
MboI GATC 2 cut(s) 131, 304
MboII GAAGA 1 cut(s) 333
MflI RGATCY 1 cut(s) 304
MluCI AATT 4 cut(s) 36, 170, 208, 360
MnlI CCTC 1 cut(s) 162
MseI TTAA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 17, 236
NdeII GATC 2 cut(s) 131, 304
NheI GCTAGC 1 cut(s) 73
NlaIV GGNNCC 2 cut(s) 117, 231
NspV TTCGAA 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PfeI GAWTC 1 cut(s) 175
PpuMI RGGWCCY 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 117, 231
PspPI GGNCC 2 cut(s) 116, 267
PspPPI RGGWCCY 1 cut(s) 116
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 5, 276
RsaNI GTAC 2 cut(s) 4, 275
SaqAI TTAA 1 cut(s) 251
Sau3AI GATC 2 cut(s) 131, 304
Sau96I GGNCC 2 cut(s) 116, 267
SetI ASST 9 cut(s) 13, 22, 79, 85, 109, 154, 190, 280, 352
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 1 cut(s) 116
Sse9I AATT 4 cut(s) 36, 170, 208, 360
SsiI CCGC 1 cut(s) 371
SspMI CTAG 5 cut(s) 53, 74, 155, 347, 381
TaiI ACGT 2 cut(s) 85, 109
TaqI TCGA 1 cut(s) 167
TaqII GACCGA 1 cut(s) 366
TasI AATT 4 cut(s) 36, 170, 208, 360
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 251
Tru9I TTAA 1 cut(s) 251
TscAI CASTG 1 cut(s) 240
TspRI CASTG 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 116
XspI CTAG 5 cut(s) 53, 74, 155, 347, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.