RchiOBHm_Chr6g0256751

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020171
Physical Location & Seq
Reverse (-)
12006898 .. 12007323
426 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23034

Sequence Viewer

Length: 300 bp
ATGGCTTTTGTGCAACGTCGAAAGGGTCCTGATGTAGTGGGAGCGTTCGGATTGTTACAACCTCTAGCGGATGGTTTGAAATTGATTCTAAAAGAACCTATTTCACCAAGTAGTGCTAATTTCTCCCTTTTTAGAATGGCTCCAGTGACTACATTTATGTTAAGTCTGGTTGCTCGGGCCGTTGTACCTTTTGATTATGGTATGGTATTGTCAGATTCGAACATAGGGCTACTTTATTTGTTTGCCATATCTTCGCTAGGTGTTTATGGAATTATTATAGCAGGTTGGTCTAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
NADHdh

Protein Analysis

99

Amino Acids

10.61

Weight (kDa)

9.3

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADHdh PF00146 1 - 99 2.2e-33 NADH dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07785 ATMG01275
rosa_chinensis RchiOBHm_Chr6g0256751 RchiOBHm_MTg0498831
rosa_roxburghii Rroxscaffold_2G00119320
rosa_samantha Rh6CG178200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AciI CCGC 1 cut(s) 68
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 26, 177
AsuHPI GGTGA 1 cut(s) 96
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 26
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 164
BfaI CTAG 3 cut(s) 65, 257, 291
BfuAI ACCTGC 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 26, 177
BmiI GGNNCC 2 cut(s) 27, 141
BpmI CTGGAG 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 143
BseGI GGATG 1 cut(s) 76
BseNI ACTGG 1 cut(s) 143
BshFI GGCC 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 174
BsnI GGCC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 218
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 179
BspLI GGNNCC 2 cut(s) 27, 141
BspMI ACCTGC 1 cut(s) 272
BspT104I TTCGAA 1 cut(s) 218
BsrI ACTGG 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 218
BstF5I GGATG 1 cut(s) 76
BsuRI GGCC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 26, 177
Csp6I GTAC 1 cut(s) 185
CviJI RGCY 4 cut(s) 5, 140, 179, 229
CviKI_1 RGCY 4 cut(s) 5, 140, 179, 229
CviQI GTAC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 26
FaiI YATR 7 cut(s) 158, 198, 203, 224, 248, 267, 278
FokI GGATG 1 cut(s) 83
FspBI CTAG 3 cut(s) 65, 257, 291
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 1 cut(s) 179
HinfI GANTC 2 cut(s) 85, 215
HphI GGTGA 1 cut(s) 96
Hpy188I TCNGA 2 cut(s) 50, 214
Hpy188III TCNNGA 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 1 cut(s) 13
HpySE526I ACGT 1 cut(s) 16
LmnI GCTCC 2 cut(s) 41, 145
LpnPI CCDG 4 cut(s) 42, 152, 156, 267
MaeI CTAG 3 cut(s) 65, 257, 291
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 2 cut(s) 54, 145
MboII GAAGA 1 cut(s) 243
MluCI AATT 4 cut(s) 80, 118, 270, 295
MnlI CCTC 1 cut(s) 72
MseI TTAA 1 cut(s) 161
NlaIV GGNNCC 2 cut(s) 27, 141
NmuCI GTSAC 1 cut(s) 145
NspV TTCGAA 1 cut(s) 218
PfeI GAWTC 2 cut(s) 85, 215
PpuMI RGGWCCY 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 27, 141
PspPI GGNCC 2 cut(s) 26, 177
PspPPI RGGWCCY 1 cut(s) 26
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 161
Sau96I GGNCC 2 cut(s) 26, 177
SetI ASST 6 cut(s) 19, 64, 100, 190, 262, 286
SfuI TTCGAA 1 cut(s) 218
SgeI CNNG 9 cut(s) 41, 77, 120, 155, 179, 186, 188, 269, 294
SinI GGWCC 1 cut(s) 26
Sse9I AATT 4 cut(s) 80, 118, 270, 295
SsiI CCGC 1 cut(s) 68
SspMI CTAG 3 cut(s) 65, 257, 291
TaiI ACGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 19, 218
TasI AATT 4 cut(s) 80, 118, 270, 295
TfiI GAWTC 2 cut(s) 85, 215
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 26
XspI CTAG 3 cut(s) 65, 257, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.