FvH4_1g00270

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017889
Physical Location & Seq
Reverse (-)
136396 .. 138572
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00270.t1

Sequence Viewer

Length: 1209 bp
ATGGCACCTAAGCTTGTATCTGCTATGTCTGTTGTTATAGCTTGTTTGGTAGCCTTGGCGTTCTTGTTTGAATCATCACATGCAGCTGGCGGTGCAACCTACAATGTGGTGAGCTACGGTGCGAGAGGAGATGGGAAAACAGACTCAACCAAAGCATTCCTCAGGGCATGGTCCGCGGCATGCTTCTCTTCTAGAAGCGCCACCGTTTACGTTCCTCGTGGCACCTTCTTGTTGAAACCTGTAGTTTTCAGCGGCCCATGCAGAAGTAGCAGAATTGTGTTTCAGATGGGAGCGGGAACCAGTACTACTCTAGTTGCCCCTTCGAATTACTGGGAACTCGGAAACACCGGAAATTGGATTTTGTTCATAAAGGTAACAGGCCTCTCTATCTACGGAGGTACAGTTGATGCTCGTGGTGCTGGATATTGGCATTGCAAGAGATATGGCAAGAGTTGCACAGCAGGAGCCAGGTCAGTATCGTTGTTTTGGTGTAGTAATGTGGTGGTGAGCGGGTTGAGGACCTTGAACAGCCAGACTATGCATATGGCAATTGACCACAGCAACAATGTACATGTTAAAAACGTGTGGATCAGAGCCCCAAGCGGTAGTCCTAACACAGACGGCATACACATTGAAGCTTCGACTGGGATTACCATTACTGCCAGCACCATAATGACCGGAGATGACTGCATATCACTTGGCTCCGGCGCAAGAAACGTGTGGATTGAACGCATAGCCTGTGGCCCTGGACATGGAATAAGCATTGGTAGTCTTGGTGGTTCTGTGAATGAAGATGGTGTGCAGAACGTGACAGTAACAAGTTCCGTCTTCACAAAAACGCAAAACGGCGTACGGATCAAATCATGGGCGAGAGCAAGCAATGGTTTTGCAAGAAACATTGTTTACCGCAACCTTATCATGAAAAATGTCGACAACCCCATCATCATCGATCAGAAATACTGCCCTGGTAACAAAGGTTGTCCTCGTCAGAGCTCTGGCGTGAAGATTAGCAAAGTGACATACAAGAACATAAGGGGCTCATCAGGCACACAAGTAGCCATGAACTTCTACTGCAGCCCCAGTAATCCGTGCTGGGGAATCAAGCTGCAAGATATAAGGCTCACTTATGGGAAAAAGGCGGCCACTTCCTCTTGTGCCAATATCGGTGGGAGCAAGAGTGGAGTGATCATACCAAGGAGTTGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

403

Amino Acids

42.68

Weight (kDa)

9.77

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 35 - 287 3e-11 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 61 - 389 3.8e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43880 AT2G43880
fragaria_vesca FvH4_1g00270
malus_domestica MD15G1143900.v1.1
prunus_persica Prupe.7G269300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084571
rosa_laevigata RLG00000015628
rosa_multiflora Rmu_sc0002352.1_g000018
rosa_rugosa Rorug01G0455100
rosa_samantha Rh2CG003300
rosa_wichuraiana Rw2G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 221
AccBSI CCGCTC 2 cut(s) 293, 510
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 176
AciI CCGC 9 cut(s) 90, 174, 176, 252, 293, 510, 603, 907, 1139
AclWI GGATC 2 cut(s) 596, 863
AcoI YGGCCR 1 cut(s) 1140
AfaI GTAC 4 cut(s) 304, 400, 570, 852
AfiI CCNNNNNNNGG 3 cut(s) 354, 752, 1094
AflIII ACRYGT 3 cut(s) 571, 582, 717
AgsI TTSAA 5 cut(s) 71, 235, 526, 635, 728
AjnI CCWGG 3 cut(s) 467, 745, 964
AluBI AGCT 7 cut(s) 13, 41, 86, 114, 638, 993, 1105
AluI AGCT 7 cut(s) 13, 41, 86, 114, 638, 993, 1105
Alw21I GWGCWC 1 cut(s) 995
AlwI GGATC 2 cut(s) 596, 863
AoxI GGCC 4 cut(s) 253, 379, 742, 1140
ApeKI GCWGC 3 cut(s) 83, 1074, 1105
AspLEI GCGC 2 cut(s) 200, 710
AspS9I GGNCC 4 cut(s) 171, 254, 519, 743
AsuHPI GGTGA 2 cut(s) 121, 517
AsuII TTCGAA 1 cut(s) 323
AvaII GGWCC 2 cut(s) 171, 519
AxyI CCTNAGG 1 cut(s) 161
BanI GGYRCC 2 cut(s) 4, 221
BanII GRGCYC 3 cut(s) 598, 995, 1040
BauI CACGAG 2 cut(s) 216, 411
BbsI GAAGAC 1 cut(s) 820
Bbv12I GWGCWC 1 cut(s) 995
BbvI GCAGC 3 cut(s) 95, 1086, 1092
BccI CCATC 4 cut(s) 125, 280, 788, 947
BceAI ACGGC 2 cut(s) 637, 862
BciT130I CCWGG 3 cut(s) 469, 747, 966
BclI TGATCA 1 cut(s) 1185
BfaI CTAG 2 cut(s) 192, 311
BfmI CTRYAG 2 cut(s) 240, 1072
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 6 cut(s) 84, 177, 253, 1075, 1106, 1140
BlsI GCNGC 6 cut(s) 85, 178, 254, 1076, 1107, 1141
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 3 cut(s) 469, 747, 966
Bme18I GGWCC 2 cut(s) 171, 519
BmgT120I GGNCC 4 cut(s) 171, 254, 519, 743
BmiI GGNNCC 5 cut(s) 6, 223, 298, 466, 703
BmrFI CCNGG 3 cut(s) 469, 747, 966
BmrI ACTGGG 3 cut(s) 340, 654, 1074
BmsI GCATC 1 cut(s) 397
BmuI ACTGGG 3 cut(s) 340, 654, 1074
BpiI GAAGAC 1 cut(s) 820
Bpu10I CCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 948
BsaJI CCNNGG 5 cut(s) 54, 174, 745, 964, 1193
BsaWI WCCGGW 2 cut(s) 347, 677
BsaXI ACNNNNNCTCC 4 cut(s) 387, 417, 1162, 1192
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 752, 1094
Bse1I ACTGG 4 cut(s) 300, 335, 649, 1080
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 430, 886
BseBI CCWGG 3 cut(s) 469, 747, 966
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 5 cut(s) 54, 174, 745, 964, 1193
BseLI CCNNNNNNNGG 3 cut(s) 354, 752, 1094
BseMI GCAATG 2 cut(s) 430, 886
BseMII CTCAG 1 cut(s) 175
BseNI ACTGG 4 cut(s) 300, 335, 649, 1080
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 3 cut(s) 95, 1086, 1092
BseYI CCCAGC 1 cut(s) 1092
BsgI GTGCAG 1 cut(s) 821
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 4 cut(s) 255, 381, 744, 1142
BshNI GGYRCC 2 cut(s) 4, 221
BshVI ATCGAT 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 995
BsiSI CCGG 3 cut(s) 348, 678, 705
BsiWI CGTACG 1 cut(s) 850
BslI CCNNNNNNNGG 3 cut(s) 354, 752, 1094
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 4 cut(s) 255, 381, 744, 1142
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 598, 995, 1040
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 4 cut(s) 588, 855, 949, 1185
BspACI CCGC 9 cut(s) 90, 174, 176, 252, 293, 510, 603, 907, 1139
BspANI GGCC 4 cut(s) 255, 381, 744, 1142
BspCNI CTCAG 1 cut(s) 174
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 5 cut(s) 6, 223, 298, 466, 703
BspMAI CTGCAG 1 cut(s) 1076
BspPI GGATC 2 cut(s) 596, 863
BspT104I TTCGAA 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 4, 221
BsrBI CCGCTC 2 cut(s) 293, 510
BsrDI GCAATG 2 cut(s) 430, 886
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 4 cut(s) 300, 335, 649, 1080
BssECI CCNNGG 5 cut(s) 54, 174, 745, 964, 1193
BssMI GATC 4 cut(s) 588, 855, 949, 1185
BssSI CACGAG 2 cut(s) 216, 411
BssT1I CCWWGG 2 cut(s) 54, 1193
Bst2BI CACGAG 2 cut(s) 216, 411
Bst2UI CCWGG 3 cut(s) 469, 747, 966
Bst4CI ACNGT 4 cut(s) 119, 205, 403, 814
Bst6I CTCTTC 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 453
BstAUI TGTACA 1 cut(s) 568
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 4 cut(s) 88, 181, 664, 877
BstDEI CTNAG 2 cut(s) 9, 161
BstDSI CCRYGG 1 cut(s) 174
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 200, 710
BstKTI GATC 4 cut(s) 591, 858, 952, 1188
BstMBI GATC 4 cut(s) 588, 855, 949, 1185
BstMWI GCNNNNNNNGC 7 cut(s) 92, 173, 258, 267, 416, 453, 1044
BstNI CCWGG 3 cut(s) 469, 747, 966
BstNSI RCATGY 3 cut(s) 83, 183, 575
BstSCI CCNGG 3 cut(s) 467, 745, 964
BstSFI CTRYAG 2 cut(s) 240, 1072
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 95, 1086, 1092
BstV2I GAAGAC 1 cut(s) 820
Bsu15I ATCGAT 1 cut(s) 948
Bsu36I CCTNAGG 1 cut(s) 161
BsuRI GGCC 4 cut(s) 255, 381, 744, 1142
BsuTUI ATCGAT 1 cut(s) 948
BtgI CCRYGG 1 cut(s) 174
Cac8I GCNNGC 4 cut(s) 88, 181, 664, 877
CciI TCATGA 1 cut(s) 918
CfoI GCGC 2 cut(s) 200, 710
Cfr13I GGNCC 4 cut(s) 171, 254, 519, 743
Cfr42I CCGCGG 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 948
Csp6I GTAC 4 cut(s) 303, 399, 569, 851
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviAII CATG 9 cut(s) 80, 168, 180, 258, 572, 752, 864, 919, 1060
CviQI GTAC 4 cut(s) 303, 399, 569, 851
DdeI CTNAG 2 cut(s) 9, 161
DpnI GATC 4 cut(s) 590, 857, 951, 1187
DpnII GATC 4 cut(s) 588, 855, 949, 1185
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 54, 1193
Eco147I AGGCCT 1 cut(s) 381
Eco24I GRGCYC 3 cut(s) 598, 995, 1040
Eco47I GGWCC 2 cut(s) 171, 519
Eco53kI GAGCTC 1 cut(s) 993
Eco81I CCTNAGG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 993
EcoO109I RGGNCCY 1 cut(s) 519
EcoRII CCWGG 3 cut(s) 467, 745, 964
EcoT14I CCWWGG 2 cut(s) 54, 1193
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 3 cut(s) 598, 995, 1040
ErhI CCWWGG 2 cut(s) 54, 1193
FaeI CATG 9 cut(s) 83, 171, 183, 261, 575, 755, 867, 922, 1063
FalI AAGNNNNNCTT 2 cut(s) 1108, 1140
FatI CATG 9 cut(s) 79, 167, 179, 257, 571, 751, 863, 918, 1059
FauI CCCGC 2 cut(s) 286, 503
FauNDI CATATG 1 cut(s) 543
FbaI TGATCA 1 cut(s) 1185
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 6 cut(s) 84, 177, 253, 1075, 1106, 1140
FriOI GRGCYC 3 cut(s) 598, 995, 1040
Fsp4HI GCNGC 6 cut(s) 84, 177, 253, 1075, 1106, 1140
FspBI CTAG 2 cut(s) 192, 311
GlaI GCGC 2 cut(s) 199, 709
GluI GCNGC 6 cut(s) 84, 177, 253, 1075, 1106, 1140
GsaI CCCAGC 1 cut(s) 1096
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 4 cut(s) 255, 381, 744, 1142
HapII CCGG 3 cut(s) 348, 678, 705
HhaI GCGC 2 cut(s) 200, 710
Hin1II CATG 9 cut(s) 83, 171, 183, 261, 575, 755, 867, 922, 1063
Hin6I GCGC 2 cut(s) 198, 708
HinP1I GCGC 2 cut(s) 198, 708
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 2 cut(s) 11, 636
HinfI GANTC 3 cut(s) 71, 143, 1098
HpaII CCGG 3 cut(s) 348, 678, 705
HphI GGTGA 2 cut(s) 121, 517
Hpy166II GTNNAC 3 cut(s) 208, 904, 931
Hpy188I TCNGA 5 cut(s) 285, 341, 593, 954, 990
Hpy188III TCNNGA 2 cut(s) 192, 919
Hpy8I GTNNAC 3 cut(s) 208, 904, 931
HpyAV CCTTC 2 cut(s) 235, 330
HpyCH4III ACNGT 4 cut(s) 119, 205, 403, 814
HpyCH4IV ACGT 4 cut(s) 210, 582, 717, 807
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 173, 258, 267, 416, 453, 1044
HpyF3I CTNAG 2 cut(s) 9, 161
HpySE526I ACGT 4 cut(s) 210, 582, 717, 807
Hsp92II CATG 9 cut(s) 83, 171, 183, 261, 575, 755, 867, 922, 1063
HspAI GCGC 2 cut(s) 198, 708
Ksp22I TGATCA 1 cut(s) 1185
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 4 cut(s) 588, 855, 949, 1185
LmnI GCTCC 4 cut(s) 290, 464, 707, 1170
Lsp1109I GCAGC 3 cut(s) 95, 1086, 1092
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 192, 311
MaeII ACGT 4 cut(s) 210, 582, 717, 807
MaeIII GTNAC 5 cut(s) 373, 808, 814, 968, 1015
MalI GATC 4 cut(s) 590, 857, 951, 1187
MbiI CCGCTC 2 cut(s) 293, 510
MboI GATC 4 cut(s) 588, 855, 949, 1185
MboII GAAGA 4 cut(s) 180, 803, 820, 1015
MfeI CAATTG 1 cut(s) 549
MhlI GDGCHC 3 cut(s) 598, 995, 1040
MluCI AATT 4 cut(s) 273, 325, 352, 549
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 8 cut(s) 119, 170, 225, 389, 392, 510, 993, 1159
Mph1103I ATGCAT 1 cut(s) 543
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 3 cut(s) 86, 176, 252
MspI CCGG 3 cut(s) 348, 678, 705
MspR9I CCNGG 3 cut(s) 469, 747, 966
MunI CAATTG 1 cut(s) 549
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 3 cut(s) 469, 747, 966
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 7 cut(s) 92, 173, 258, 267, 416, 453, 1044
NdeI CATATG 1 cut(s) 543
NdeII GATC 4 cut(s) 588, 855, 949, 1185
NlaIII CATG 9 cut(s) 83, 171, 183, 261, 575, 755, 867, 922, 1063
NlaIV GGNNCC 5 cut(s) 6, 223, 298, 466, 703
NmuCI GTSAC 2 cut(s) 808, 1015
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 3 cut(s) 83, 183, 575
NspV TTCGAA 1 cut(s) 323
PaeI GCATGC 1 cut(s) 183
PagI TCATGA 1 cut(s) 918
PceI AGGCCT 1 cut(s) 381
PciI ACATGT 1 cut(s) 571
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 71, 1098
Pfl23II CGTACG 1 cut(s) 850
PkrI GCNGC 6 cut(s) 85, 178, 254, 1076, 1107, 1141
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 519
PscI ACATGT 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 995
Psp5II RGGWCCY 1 cut(s) 519
Psp6I CCWGG 3 cut(s) 467, 745, 964
PspFI CCCAGC 1 cut(s) 1092
PspGI CCWGG 3 cut(s) 467, 745, 964
PspLI CGTACG 1 cut(s) 850
PspN4I GGNNCC 5 cut(s) 6, 223, 298, 466, 703
PspPI GGNCC 4 cut(s) 171, 254, 519, 743
PspPPI RGGWCCY 1 cut(s) 519
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PstI CTGCAG 1 cut(s) 1076
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 4 cut(s) 304, 400, 570, 852
RsaNI GTAC 4 cut(s) 303, 399, 569, 851
RseI CAYNNNNRTG 1 cut(s) 671
SacI GAGCTC 1 cut(s) 995
SacII CCGCGG 1 cut(s) 177
SalI GTCGAC 1 cut(s) 929
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 6 cut(s) 84, 177, 253, 1075, 1106, 1140
Sau3AI GATC 4 cut(s) 588, 855, 949, 1185
Sau96I GGNCC 4 cut(s) 171, 254, 519, 743
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 469, 747, 966
SduI GDGCHC 3 cut(s) 598, 995, 1040
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 2 cut(s) 240, 1072
Sfr303I CCGCGG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 323
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 171, 519
SmiMI CAYNNNNRTG 1 cut(s) 671
SphI GCATGC 1 cut(s) 183
Sse9I AATT 4 cut(s) 273, 325, 352, 549
SseBI AGGCCT 1 cut(s) 381
SsiI CCGC 9 cut(s) 90, 174, 176, 252, 293, 510, 603, 907, 1139
SspMI CTAG 2 cut(s) 192, 311
SstI GAGCTC 1 cut(s) 995
StuI AGGCCT 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 467, 745, 964
StyI CCWWGG 2 cut(s) 54, 1193
TaaI ACNGT 4 cut(s) 119, 205, 403, 814
TaiI ACGT 4 cut(s) 213, 585, 720, 810
TaqI TCGA 4 cut(s) 323, 641, 930, 948
TasI AATT 4 cut(s) 273, 325, 352, 549
TatI WGTACW 2 cut(s) 302, 568
TauI GCSGC 3 cut(s) 179, 255, 1142
TfiI GAWTC 2 cut(s) 71, 1098
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TseFI GTSAC 2 cut(s) 808, 1015
TseI GCWGC 3 cut(s) 83, 1074, 1105
Tsp45I GTSAC 2 cut(s) 808, 1015
TspDTI ATGAA 4 cut(s) 355, 804, 935, 1076
TspGWI ACGGA 4 cut(s) 408, 814, 868, 1077
VpaK11BI GGWCC 2 cut(s) 171, 519
XbaI TCTAGA 1 cut(s) 191
XceI RCATGY 3 cut(s) 83, 183, 575
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 2 cut(s) 192, 311
ZrmI AGTACT 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.