MD15G1143900.v1.1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017889
Physical Location & Seq
Reverse (-)
10625549 .. 10627554
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1143900.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGCGCCTAAGATTGTTGTTATTTTGGTGTTTTTGATATCCTTGTTTGAATCATCGTCAAATTACTATGCAGTGGGTGCATCGTATAATGTGGCTAGGTACGGTGCAAGAGGAGATGGGAGAACAGACTCAACCAAAGCATTTCTTAGGGCATGGTCATTGGCATGCTCGTCGACAGCTAAGGGCGGAGCCACCTTGTACGTTCCTCGAGGCAGTTTCTTGATAAAGCCGGTAGTGTTTAGTGGCCCATGCAGAAGCAGCAGAGTTGTGTTTCAGATGGATGCGGCGGCTAGTCTAGTTGCCCCAACTAATTATTGGGAACTTGGAAACACTGGAACTTGGATTTTGTTCATAAGGGTCACAGGGCTCCTTGTGTACGGAGGGACCGTTGATGCTAGGGCAAATTCATATTGGCAATGCAGGAGATCCGGCAAGAGTTGCACAGCAGGATCAAGGTCAGTGGCGTTGTATTGGTGTAATAATGCGGTGGTGCGCGGGTTAAGAATGATGAACAGCCAGATAATGCATATGGCAATCGACAACTGCAAGAATGTGGCAGTGAGAAACGTATATATAAGAGCCCCAAGTGGAAGCCCAAATACGGATGGCATCCACATAGAAGCTTCGACGGGGGTCACCATCACTGCCTCTTCTATTATGACTGGAGACGACTGCATATCAATCGGCGAAGGCGCCAAGAATTTGTGGATTGAACGCATTGCCTGTGGCCCTGGCCATGGAATAAGCATTGGGAGTCTTGGTGCATCTGCAAATGAAGATGGAGTTCAAAATGTGACGGTAACAAATTCGGTCTTCACTAAAACCCAAAACGGCGTGCGGATCAAAACATGGGCAAGAGCTAGCAATAGTTTTGTAACAAACATTTCTTTTCGGAACCTTATCATGAAAAACGTCGGCAACCCCATCATGATTGATCAAAAGTACTGCCCTAGTAATCAAGGCTGTCCTCGTCAGAATTCTGGTGTGAAGATTAGTAGAGTTACGTACACGAATATAAGGGGAACATCTACGGCGCCAATAGCCATGAATTTCTTTTGCAGCCCTAGTAATCCCTGCTGGGGGCTGAAAGTACGAGACATAAGGCTTACTTATTTCAAGACAGCAGCCAAGTCTTACTGTGCCAATGCAGGTGGGAGCAGTAGCGGAGTGGTCATACCAAGGAGTTGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

398

Amino Acids

42.65

Weight (kDa)

9.78

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 29 - 78 1.9e-09 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 64 - 383 5.1e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017889)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43880 AT2G43880
fragaria_vesca FvH4_1g00270
malus_domestica MD15G1143900.v1.1
prunus_persica Prupe.7G269300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084571
rosa_laevigata RLG00000015628
rosa_multiflora Rmu_sc0002352.1_g000018
rosa_rugosa Rorug01G0455100
rosa_samantha Rh2CG003300
rosa_wichuraiana Rw2G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1139
AbsI CCTCGAGG 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 1139
AccB1I GGYRCC 3 cut(s) 4, 692, 1033
AccI GTMKAC 1 cut(s) 173
AccII CGCG 1 cut(s) 495
AciI CCGC 7 cut(s) 186, 284, 287, 485, 495, 838, 1164
AclWI GGATC 3 cut(s) 420, 457, 848
AcoI YGGCCR 1 cut(s) 733
AcsI RAATTY 5 cut(s) 403, 700, 805, 976, 1048
AcyI GRCGYC 3 cut(s) 5, 693, 1034
AfaI GTAC 6 cut(s) 101, 200, 377, 944, 1007, 1092
AfiI CCNNNNNNNGG 4 cut(s) 601, 737, 1079, 1080
AgsI TTSAA 4 cut(s) 50, 713, 788, 1117
AjnI CCWGG 1 cut(s) 730
AluBI AGCT 3 cut(s) 179, 623, 860
AluI AGCT 3 cut(s) 179, 623, 860
Alw26I GTCTC 2 cut(s) 660, 1089
AlwI GGATC 3 cut(s) 420, 457, 848
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 3 cut(s) 244, 727, 733
ApeKI GCWGC 3 cut(s) 258, 1059, 1124
ApoI RAATTY 5 cut(s) 403, 700, 805, 976, 1048
AspLEI GCGC 4 cut(s) 7, 495, 695, 1036
AspS9I GGNCC 3 cut(s) 245, 384, 728
AsuHPI GGTGA 1 cut(s) 628
AsuNHI GCTAGC 1 cut(s) 860
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 384
BalI TGGCCA 1 cut(s) 735
BanI GGYRCC 3 cut(s) 4, 692, 1033
BanII GRGCYC 2 cut(s) 369, 583
BarI GAAGNNNNNNTAC 2 cut(s) 583, 615
BbsI GAAGAC 1 cut(s) 805
BbvI GCAGC 3 cut(s) 270, 1071, 1136
BccI CCATC 6 cut(s) 110, 271, 599, 647, 773, 932
BceAI ACGGC 2 cut(s) 847, 1047
BcgI CGANNNNNNTGC 4 cut(s) 153, 187, 664, 698
BciT130I CCWGG 1 cut(s) 732
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 2 cut(s) 660, 1089
BfaI CTAG 7 cut(s) 96, 291, 296, 396, 861, 951, 1065
BfoI RGCGCY 3 cut(s) 8, 696, 1037
BfuAI ACCTGC 1 cut(s) 1139
BisI GCNGC 5 cut(s) 259, 285, 288, 1060, 1125
BlsI GCNGC 5 cut(s) 260, 286, 289, 1061, 1126
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 1 cut(s) 732
Bme18I GGWCC 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 245, 384, 728
BmiI GGNNCC 7 cut(s) 6, 190, 368, 385, 694, 896, 1035
BmrFI CCNGG 1 cut(s) 732
BmsI GCATC 5 cut(s) 89, 271, 382, 618, 773
BmtI GCTAGC 1 cut(s) 864
BoxI GACNNNNGTC 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 805
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 1005
BsaHI GRCGYC 3 cut(s) 5, 693, 1034
BsaJI CCNNGG 3 cut(s) 730, 736, 1178
BsaXI ACNNNNNCTCC 4 cut(s) 372, 402, 745, 775
Bsc4I CCNNNNNNNGG 4 cut(s) 601, 737, 1079, 1080
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 2 cut(s) 337, 667
Bse3DI GCAATG 2 cut(s) 422, 717
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 3 cut(s) 730, 736, 1178
BseGI GGATG 3 cut(s) 286, 609, 610
BseLI CCNNNNNNNGG 4 cut(s) 601, 737, 1079, 1080
BseMI GCAATG 2 cut(s) 422, 717
BseNI ACTGG 2 cut(s) 337, 667
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 3 cut(s) 270, 1071, 1136
BseYI CCCAGC 1 cut(s) 1077
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 3 cut(s) 246, 729, 735
BshNI GGYRCC 3 cut(s) 4, 692, 1033
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 2 cut(s) 230, 429
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 4 cut(s) 601, 737, 1079, 1080
BsmAI GTCTC 2 cut(s) 660, 1089
BsmBI CGTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 3 cut(s) 246, 729, 735
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 2 cut(s) 369, 583
Bsp143I GATC 4 cut(s) 425, 449, 840, 934
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 7 cut(s) 186, 284, 287, 485, 495, 838, 1164
BspANI GGCC 3 cut(s) 246, 729, 735
BspFNI CGCG 1 cut(s) 495
BspHI TCATGA 2 cut(s) 903, 927
BspLI GGNNCC 7 cut(s) 6, 190, 368, 385, 694, 896, 1035
BspMI ACCTGC 1 cut(s) 1139
BspOI GCTAGC 1 cut(s) 864
BspPI GGATC 3 cut(s) 420, 457, 848
BspT107I GGYRCC 3 cut(s) 4, 692, 1033
BsrDI GCAATG 2 cut(s) 422, 717
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 2 cut(s) 337, 667
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 730, 736, 1178
BssMI GATC 4 cut(s) 425, 449, 840, 934
BssNI GRCGYC 3 cut(s) 5, 693, 1034
BssT1I CCWWGG 2 cut(s) 736, 1178
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 4 cut(s) 104, 388, 799, 1139
Bst6I CTCTTC 1 cut(s) 655
BstACI GRCGYC 3 cut(s) 5, 693, 1034
BstAPI GCANNNNNTGC 2 cut(s) 77, 438
BstBAI YACGTR 1 cut(s) 1005
BstC8I GCNNGC 3 cut(s) 166, 836, 862
BstDEI CTNAG 3 cut(s) 9, 146, 180
BstDSI CCRYGG 1 cut(s) 736
BstEII GGTNACC 1 cut(s) 634
BstF5I GGATG 3 cut(s) 286, 609, 610
BstFNI CGCG 1 cut(s) 495
BstH2I RGCGCY 3 cut(s) 8, 696, 1037
BstHHI GCGC 4 cut(s) 7, 495, 695, 1036
BstKTI GATC 4 cut(s) 428, 452, 843, 937
BstMAI GTCTC 2 cut(s) 660, 1089
BstMBI GATC 4 cut(s) 425, 449, 840, 934
BstMWI GCNNNNNNNGC 4 cut(s) 77, 258, 438, 1040
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 1 cut(s) 168
BstPAI GACNNNNGTC 1 cut(s) 632
BstPI GGTNACC 1 cut(s) 634
BstSCI CCNGG 1 cut(s) 730
BstSNI TACGTA 1 cut(s) 1005
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 270, 1071, 1136
BstV2I GAAGAC 1 cut(s) 805
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BsuRI GGCC 3 cut(s) 246, 729, 735
BtgI CCRYGG 1 cut(s) 736
BtsCI GGATG 3 cut(s) 286, 609, 610
BtsI GCAGTG 3 cut(s) 78, 564, 642
BtsIMutI CAGTG 5 cut(s) 78, 330, 465, 564, 642
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 3 cut(s) 166, 836, 862
CciI TCATGA 2 cut(s) 903, 927
CfoI GCGC 4 cut(s) 7, 495, 695, 1036
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 3 cut(s) 245, 384, 728
Csp6I GTAC 6 cut(s) 100, 199, 376, 943, 1006, 1091
CspCI CAANNNNNGTGG 4 cut(s) 441, 476, 1132, 1167
CviAII CATG 8 cut(s) 153, 165, 249, 737, 849, 904, 928, 1045
CviQI GTAC 6 cut(s) 100, 199, 376, 943, 1006, 1091
DdeI CTNAG 3 cut(s) 9, 146, 180
DinI GGCGCC 3 cut(s) 6, 694, 1035
DpnI GATC 4 cut(s) 427, 451, 842, 936
DpnII GATC 4 cut(s) 425, 449, 840, 934
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 1 cut(s) 655
EarI CTCTTC 1 cut(s) 655
EciI GGCGGA 1 cut(s) 201
Eco105I TACGTA 1 cut(s) 1005
Eco130I CCWWGG 2 cut(s) 736, 1178
Eco24I GRGCYC 2 cut(s) 369, 583
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 730
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 2 cut(s) 736, 1178
EcoT22I ATGCAT 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 369, 583
EgeI GGCGCC 3 cut(s) 6, 694, 1035
EheI GGCGCC 3 cut(s) 6, 694, 1035
ErhI CCWWGG 2 cut(s) 736, 1178
Esp3I CGTCTC 1 cut(s) 660
FaeI CATG 8 cut(s) 156, 168, 252, 740, 852, 907, 931, 1048
FalI AAGNNNNNCTT 4 cut(s) 129, 161, 1093, 1125
FaqI GGGAC 1 cut(s) 397
FatI CATG 8 cut(s) 152, 164, 248, 736, 848, 903, 927, 1044
FauI CCCGC 1 cut(s) 488
FauNDI CATATG 1 cut(s) 528
FbaI TGATCA 1 cut(s) 934
FblI GTMKAC 1 cut(s) 173
Fnu4HI GCNGC 5 cut(s) 259, 285, 288, 1060, 1125
FokI GGATG 3 cut(s) 293, 596, 617
FriOI GRGCYC 2 cut(s) 369, 583
Fsp4HI GCNGC 5 cut(s) 259, 285, 288, 1060, 1125
FspBI CTAG 7 cut(s) 96, 291, 296, 396, 861, 951, 1065
GlaI GCGC 4 cut(s) 6, 494, 694, 1035
GluI GCNGC 5 cut(s) 259, 285, 288, 1060, 1125
GsaI CCCAGC 1 cut(s) 1081
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 3 cut(s) 8, 696, 1037
HaeIII GGCC 3 cut(s) 246, 729, 735
HapII CCGG 2 cut(s) 230, 429
HhaI GCGC 4 cut(s) 7, 495, 695, 1036
Hin1I GRCGYC 3 cut(s) 5, 693, 1034
Hin1II CATG 8 cut(s) 156, 168, 252, 740, 852, 907, 931, 1048
Hin6I GCGC 4 cut(s) 5, 493, 693, 1034
HinP1I GCGC 4 cut(s) 5, 493, 693, 1034
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 3 cut(s) 50, 128, 754
HpaII CCGG 2 cut(s) 230, 429
HphI GGTGA 1 cut(s) 628
Hpy166II GTNNAC 3 cut(s) 174, 376, 1008
Hpy188I TCNGA 4 cut(s) 276, 894, 975, 1193
Hpy188III TCNNGA 4 cut(s) 220, 904, 928, 1117
Hpy8I GTNNAC 3 cut(s) 174, 376, 1008
Hpy99I CGWCG 3 cut(s) 175, 631, 917
HpyAV CCTTC 1 cut(s) 683
HpyCH4III ACNGT 4 cut(s) 104, 388, 799, 1139
HpyCH4IV ACGT 4 cut(s) 201, 567, 912, 1004
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 258, 438, 1040
HpyF3I CTNAG 3 cut(s) 9, 146, 180
HpySE526I ACGT 4 cut(s) 201, 567, 912, 1004
Hsp92I GRCGYC 3 cut(s) 5, 693, 1034
Hsp92II CATG 8 cut(s) 156, 168, 252, 740, 852, 907, 931, 1048
HspAI GCGC 4 cut(s) 5, 493, 693, 1034
KasI GGCGCC 3 cut(s) 4, 692, 1033
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 4 cut(s) 425, 449, 840, 934
LmnI GCTCC 3 cut(s) 188, 372, 1155
Lsp1109I GCAGC 3 cut(s) 270, 1071, 1136
LweI GCATC 5 cut(s) 89, 271, 382, 618, 773
MaeI CTAG 7 cut(s) 96, 291, 296, 396, 861, 951, 1065
MaeII ACGT 4 cut(s) 201, 567, 912, 1004
MaeIII GTNAC 6 cut(s) 358, 634, 793, 799, 874, 1000
MalI GATC 4 cut(s) 427, 451, 842, 936
MboI GATC 4 cut(s) 425, 449, 840, 934
MboII GAAGA 4 cut(s) 642, 788, 805, 1000
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 2 cut(s) 369, 583
MlsI TGGCCA 1 cut(s) 735
MluCI AATT 7 cut(s) 61, 310, 403, 700, 805, 976, 1048
MluNI TGGCCA 1 cut(s) 735
Mly113I GGCGCC 3 cut(s) 5, 693, 1034
MlyI GAGTC 2 cut(s) 122, 763
MnlI CCTC 6 cut(s) 104, 203, 216, 374, 658, 978
Mox20I TGGCCA 1 cut(s) 735
Mph1103I ATGCAT 1 cut(s) 528
MscI TGGCCA 1 cut(s) 735
MseI TTAA 1 cut(s) 500
MslI CAYNNNNRTG 1 cut(s) 163
Msp20I TGGCCA 1 cut(s) 735
MspI CCGG 2 cut(s) 230, 429
MspR9I CCNGG 1 cut(s) 732
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 4 cut(s) 77, 258, 438, 1040
NarI GGCGCC 3 cut(s) 5, 693, 1034
NcoI CCATGG 1 cut(s) 736
NdeI CATATG 1 cut(s) 528
NdeII GATC 4 cut(s) 425, 449, 840, 934
NheI GCTAGC 1 cut(s) 860
NlaIII CATG 8 cut(s) 156, 168, 252, 740, 852, 907, 931, 1048
NlaIV GGNNCC 7 cut(s) 6, 190, 368, 385, 694, 896, 1035
NmuCI GTSAC 3 cut(s) 358, 634, 793
NsiI ATGCAT 1 cut(s) 528
NspI RCATGY 1 cut(s) 168
PaeI GCATGC 1 cut(s) 168
PaeR7I CTCGAG 1 cut(s) 207
PagI TCATGA 2 cut(s) 903, 927
PaqCI CACCTGC 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 5 cut(s) 260, 286, 289, 1061, 1126
PleI GAGTC 2 cut(s) 122, 762
PluTI GGCGCC 3 cut(s) 8, 696, 1037
PpsI GAGTC 2 cut(s) 122, 762
Ppu21I YACGTR 1 cut(s) 1005
PshAI GACNNNNGTC 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 730
PspEI GGTNACC 1 cut(s) 634
PspFI CCCAGC 1 cut(s) 1077
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 7 cut(s) 6, 190, 368, 385, 694, 896, 1035
PspPI GGNCC 3 cut(s) 245, 384, 728
PspXI VCTCGAGB 1 cut(s) 207
PsuI RGATCY 1 cut(s) 425
RsaI GTAC 6 cut(s) 101, 200, 377, 944, 1007, 1092
RsaNI GTAC 6 cut(s) 100, 199, 376, 943, 1006, 1091
RseI CAYNNNNRTG 1 cut(s) 163
SalI GTCGAC 1 cut(s) 172
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 5 cut(s) 259, 285, 288, 1060, 1125
Sau3AI GATC 4 cut(s) 425, 449, 840, 934
Sau96I GGNCC 3 cut(s) 245, 384, 728
ScaI AGTACT 1 cut(s) 944
SchI GAGTC 2 cut(s) 122, 763
ScrFI CCNGG 1 cut(s) 732
SduI GDGCHC 2 cut(s) 369, 583
SfaNI GCATC 5 cut(s) 89, 271, 382, 618, 773
SfoI GGCGCC 3 cut(s) 6, 694, 1035
Sfr274I CTCGAG 1 cut(s) 207
SinI GGWCC 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
SnaBI TACGTA 1 cut(s) 1005
SphI GCATGC 1 cut(s) 168
Sse9I AATT 7 cut(s) 61, 310, 403, 700, 805, 976, 1048
SsiI CCGC 7 cut(s) 186, 284, 287, 485, 495, 838, 1164
SspDI GGCGCC 3 cut(s) 4, 692, 1033
SspMI CTAG 7 cut(s) 96, 291, 296, 396, 861, 951, 1065
StyD4I CCNGG 1 cut(s) 730
StyI CCWWGG 2 cut(s) 736, 1178
TaaI ACNGT 4 cut(s) 104, 388, 799, 1139
TaiI ACGT 4 cut(s) 204, 570, 915, 1007
TaqI TCGA 4 cut(s) 173, 208, 537, 626
TaqII GACCGA 1 cut(s) 799
TasI AATT 7 cut(s) 61, 310, 403, 700, 805, 976, 1048
TatI WGTACW 1 cut(s) 942
TauI GCSGC 2 cut(s) 287, 290
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TscAI CASTG 5 cut(s) 78, 337, 465, 564, 649
TseFI GTSAC 3 cut(s) 358, 634, 793
TseI GCWGC 3 cut(s) 258, 1059, 1124
Tsp45I GTSAC 3 cut(s) 358, 634, 793
TspDTI ATGAA 6 cut(s) 340, 396, 524, 789, 920, 1061
TspGWI ACGGA 2 cut(s) 393, 617
TspRI CASTG 5 cut(s) 78, 337, 465, 564, 649
VpaK11BI GGWCC 1 cut(s) 384
XapI RAATTY 5 cut(s) 403, 700, 805, 976, 1048
XceI RCATGY 1 cut(s) 168
XcmI CCANNNNNNNNNTGG 1 cut(s) 312
XhoI CTCGAG 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 173
XspI CTAG 7 cut(s) 96, 291, 296, 396, 861, 951, 1065
ZrmI AGTACT 1 cut(s) 944
Zsp2I ATGCAT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.