FvH4_2g13880
HSP70 Family

succinate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0023593
Physical Location & Seq
Reverse (-)
12152645 .. 12155844
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13880.t1

Sequence Viewer

Length: 924 bp
ATGCCTCTTCTAAGTAAAGCTGGGAGTATACTTAGGCAGACTGCAAACAGGCAGGTCAAATCTGAGTTATCGTCTTTCAAATTGTCTATCCATCAGGCAATAAGATGCACATCATCCATGGGAAGCTCAGAAGCCTTGAGAGGAGAAACAGTCATGGACCATGACACTGGAAGATCTACAGGACTTGGATTCATCACTTCTAGTGCCATCCAGCCCTTGGGCGGGCAGGAGCTCCATGGTCACCAAGTAAGGATGAATTTTGCTACTGATAGGCCTCGAAGTAATTTTGGAGGTTCTTATGGTGTTTACACAGGAGGTGTAGTAGGTTACTATGGTCAACATGGTTCTGGATATGGTTTTGGTGTTGGTTATGGTTTGAGGAGTTATGGAGGTGGCGCCGGTGACAAGGTTTTATCCACTGGTAAGAGCAGCAACAATGGTCCTGTTGCTGGTGTTGGTAGTGCTGTCAACACTACTATTGGTAGTTCTGATAAAATCGGTGTTGGTCAGAGAAACAGCTTTTCCAGTGGAGCTACTGCAAACACTGGTGGTGCATATGAAATTGCCTACACCAGAAGTGGGGCGCGCACAGCTAATGAAGCTGGAGTCGTACCCAATACTCTTCGTCCGTTATCCCCCCATCTTCCTGTATACAAGCCACAAATCAATTCGACATTTTCAATACTTGACAGAATCGCAGCATCTTACCTAACCGCTTTATTTGTGGTGTACTCTCTTGTTTGTCTGAAAATGGGTCCTATTTGCTTCACCTTTGAGCCTTTCTACAAATTCCTGTTTTATTCTGTAAACCTTGTCCCCCTCTCCCTTGAGCTTGCTGCTTTAGCAATGGCCTATCATACGTATCATGGGATCTGTCATCTATGGGCCATTAGGGGATCTAGGTTTCTTAGATCCCGAAAGTAG

Protein Analysis

308

Amino Acids

32.64

Weight (kDa)

9.76

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sdh_cyt PF01127 209 - 295 3.2e-08 Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023593)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46505
fragaria_vesca FvH4_2g13880
rosa_chinensis RchiOBHm_Chr6g0257981

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 217
AccI GTMKAC 2 cut(s) 28, 651
AccII CGCG 1 cut(s) 586
AciI CCGC 2 cut(s) 222, 714
AclWI GGATC 3 cut(s) 878, 904, 906
AcsI RAATTY 2 cut(s) 256, 788
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 2 cut(s) 612, 731
AfiI CCNNNNNNNGG 5 cut(s) 217, 221, 222, 449, 579
AgsI TTSAA 2 cut(s) 79, 681
AluBI AGCT 8 cut(s) 20, 126, 232, 519, 533, 593, 602, 832
AluI AGCT 8 cut(s) 20, 126, 232, 519, 533, 593, 602, 832
Alw21I GWGCWC 1 cut(s) 234
AlwI GGATC 3 cut(s) 878, 904, 906
AoxI GGCC 3 cut(s) 272, 849, 885
ApeKI GCWGC 3 cut(s) 429, 698, 836
ApoI RAATTY 2 cut(s) 256, 788
AspLEI GCGC 3 cut(s) 398, 586, 588
AspS9I GGNCC 4 cut(s) 157, 440, 755, 885
AsuHPI GGTGA 3 cut(s) 233, 413, 760
AvaII GGWCC 3 cut(s) 157, 440, 755
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 441, 710, 823
BccI CCATC 3 cut(s) 99, 215, 648
BfaI CTAG 2 cut(s) 201, 900
BfmI CTRYAG 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 173
BisI GCNGC 3 cut(s) 430, 699, 837
BlsI GCNGC 3 cut(s) 431, 700, 838
Bme18I GGWCC 3 cut(s) 157, 440, 755
BmgT120I GGNCC 4 cut(s) 157, 440, 755, 885
BmiI GGNNCC 2 cut(s) 397, 756
BmsI GCATC 2 cut(s) 95, 710
BpmI CTGGAG 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 157, 848
BsaAI YACGTR 1 cut(s) 861
BsaBI GATNNNNATC 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 117, 216, 235
BsaXI ACNNNNNCTCC 4 cut(s) 135, 165, 306, 336
Bsc4I CCNNNNNNNGG 5 cut(s) 217, 221, 222, 449, 579
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 4 cut(s) 172, 424, 525, 550
Bse3DI GCAATG 1 cut(s) 852
Bse8I GATNNNNATC 1 cut(s) 109
BseDI CCNNGG 3 cut(s) 117, 216, 235
BseGI GGATG 3 cut(s) 113, 207, 258
BseJI GATNNNNATC 1 cut(s) 109
BseLI CCNNNNNNNGG 5 cut(s) 217, 221, 222, 449, 579
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 54, 141
BseNI ACTGG 4 cut(s) 172, 424, 525, 550
BsePI GCGCGC 1 cut(s) 584
BseRI GAGGAG 2 cut(s) 156, 394
BseXI GCAGC 3 cut(s) 441, 710, 823
BseYI CCCAGC 1 cut(s) 20
Bsh1236I CGCG 1 cut(s) 586
BshFI GGCC 3 cut(s) 274, 851, 887
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 800
BslI CCNNNNNNNGG 5 cut(s) 217, 221, 222, 449, 579
BsmFI GGGAC 1 cut(s) 800
BsnI GGCC 3 cut(s) 274, 851, 887
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 4 cut(s) 173, 870, 896, 911
Bsp19I CCATGG 2 cut(s) 117, 235
BspACI CCGC 2 cut(s) 222, 714
BspANI GGCC 3 cut(s) 274, 851, 887
BspCNI CTCAG 2 cut(s) 55, 140
BspFNI CGCG 1 cut(s) 586
BspLI GGNNCC 2 cut(s) 397, 756
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 3 cut(s) 878, 904, 906
BspT107I GGYRCC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 4 cut(s) 172, 424, 525, 550
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 117, 216, 235
BssHII GCGCGC 1 cut(s) 584
BssMI GATC 4 cut(s) 173, 870, 896, 911
BssNAI GTATAC 2 cut(s) 29, 652
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 3 cut(s) 117, 216, 235
Bst1107I GTATAC 2 cut(s) 29, 652
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 2 cut(s) 12, 627
BstACI GRCGYC 1 cut(s) 396
BstBAI YACGTR 1 cut(s) 861
BstC8I GCNNGC 3 cut(s) 224, 586, 834
BstDEI CTNAG 5 cut(s) 11, 32, 63, 127, 908
BstDSI CCRYGG 2 cut(s) 117, 235
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 3 cut(s) 113, 207, 258
BstFNI CGCG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 3 cut(s) 398, 586, 588
BstKTI GATC 4 cut(s) 176, 873, 899, 914
BstMBI GATC 4 cut(s) 173, 870, 896, 911
BstMWI GCNNNNNNNGC 3 cut(s) 590, 599, 842
BstPI GGTNACC 1 cut(s) 239
BstSFI CTRYAG 1 cut(s) 177
BstSNI TACGTA 1 cut(s) 861
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 3 cut(s) 441, 710, 823
BstX2I RGATCY 4 cut(s) 173, 870, 896, 911
BstXI CCANNNNNNTGG 1 cut(s) 167
BstYI RGATCY 4 cut(s) 173, 870, 896, 911
BstZ17I GTATAC 2 cut(s) 29, 652
BsuRI GGCC 3 cut(s) 274, 851, 887
BtgI CCRYGG 2 cut(s) 117, 235
BtsCI GGATG 3 cut(s) 113, 207, 258
BtsIMutI CAGTG 4 cut(s) 165, 417, 532, 543
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 3 cut(s) 224, 586, 834
CfoI GCGC 3 cut(s) 398, 586, 588
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 4 cut(s) 157, 440, 755, 885
Csp6I GTAC 2 cut(s) 611, 730
CviAII CATG 6 cut(s) 118, 154, 161, 236, 341, 866
CviQI GTAC 2 cut(s) 611, 730
DdeI CTNAG 5 cut(s) 11, 32, 63, 127, 908
DinI GGCGCC 1 cut(s) 397
DpnI GATC 4 cut(s) 175, 872, 898, 913
DpnII GATC 4 cut(s) 173, 870, 896, 911
Eam1104I CTCTTC 2 cut(s) 12, 627
EarI CTCTTC 2 cut(s) 12, 627
Ecl136II GAGCTC 1 cut(s) 232
Eco105I TACGTA 1 cut(s) 861
Eco130I CCWWGG 3 cut(s) 117, 216, 235
Eco147I AGGCCT 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 3 cut(s) 157, 440, 755
Eco53kI GAGCTC 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 755
EcoO65I GGTNACC 1 cut(s) 239
EcoT14I CCWWGG 3 cut(s) 117, 216, 235
EcoT38I GRGCYC 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 3 cut(s) 117, 216, 235
FaeI CATG 6 cut(s) 121, 157, 164, 239, 344, 869
FaqI GGGAC 1 cut(s) 800
FatI CATG 6 cut(s) 117, 153, 160, 235, 340, 865
FauI CCCGC 1 cut(s) 215
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 2 cut(s) 28, 651
Fnu4HI GCNGC 3 cut(s) 430, 699, 837
FokI GGATG 3 cut(s) 100, 194, 265
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 3 cut(s) 430, 699, 837
FspBI CTAG 2 cut(s) 201, 900
GlaI GCGC 3 cut(s) 397, 585, 587
GluI GCNGC 3 cut(s) 430, 699, 837
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 624
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 3 cut(s) 274, 851, 887
HapII CCGG 1 cut(s) 399
HhaI GCGC 3 cut(s) 398, 586, 588
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 6 cut(s) 121, 157, 164, 239, 344, 869
Hin6I GCGC 3 cut(s) 396, 584, 586
HinP1I GCGC 3 cut(s) 396, 584, 586
HincII GTYRAC 2 cut(s) 338, 469
HindII GTYRAC 2 cut(s) 338, 469
HinfI GANTC 3 cut(s) 189, 606, 693
HpaII CCGG 1 cut(s) 399
HphI GGTGA 3 cut(s) 233, 413, 760
Hpy166II GTNNAC 7 cut(s) 29, 307, 338, 469, 652, 730, 808
Hpy188I TCNGA 5 cut(s) 64, 130, 490, 510, 747
Hpy188III TCNNGA 2 cut(s) 348, 915
Hpy8I GTNNAC 7 cut(s) 29, 307, 338, 469, 652, 730, 808
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 860
HpyCH4V TGCA 4 cut(s) 44, 108, 539, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 590, 599, 842
HpyF3I CTNAG 5 cut(s) 11, 32, 63, 127, 908
HpySE526I ACGT 1 cut(s) 860
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 6 cut(s) 121, 157, 164, 239, 344, 869
HspAI GCGC 3 cut(s) 396, 584, 586
KasI GGCGCC 1 cut(s) 395
Kzo9I GATC 4 cut(s) 173, 870, 896, 911
LmnI GCTCC 3 cut(s) 229, 237, 530
Lsp1109I GCAGC 3 cut(s) 441, 710, 823
LweI GCATC 2 cut(s) 95, 710
MaeI CTAG 2 cut(s) 201, 900
MaeII ACGT 1 cut(s) 860
MaeIII GTNAC 3 cut(s) 239, 326, 401
MalI GATC 4 cut(s) 175, 872, 898, 913
MboI GATC 4 cut(s) 173, 870, 896, 911
MboII GAAGA 3 cut(s) 183, 614, 635
MflI RGATCY 4 cut(s) 173, 870, 896, 911
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 6 cut(s) 80, 256, 283, 561, 667, 788
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 615
MnlI CCTC 8 cut(s) 15, 134, 284, 285, 308, 372, 383, 830
MspI CCGG 1 cut(s) 399
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 3 cut(s) 590, 599, 842
NarI GGCGCC 1 cut(s) 396
NcoI CCATGG 2 cut(s) 117, 235
NdeI CATATG 1 cut(s) 556
NdeII GATC 4 cut(s) 173, 870, 896, 911
NlaIII CATG 6 cut(s) 121, 157, 164, 239, 344, 869
NlaIV GGNNCC 2 cut(s) 397, 756
NmuCI GTSAC 2 cut(s) 239, 401
PauI GCGCGC 1 cut(s) 584
PceI AGGCCT 1 cut(s) 274
PfeI GAWTC 2 cut(s) 189, 693
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 3 cut(s) 431, 700, 838
PleI GAGTC 1 cut(s) 614
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 614
Ppu21I YACGTR 1 cut(s) 861
PpuMI RGGWCCY 1 cut(s) 755
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 755
PspEI GGTNACC 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 397, 756
PspPI GGNCC 4 cut(s) 157, 440, 755, 885
PspPPI RGGWCCY 1 cut(s) 755
PsuI RGATCY 4 cut(s) 173, 870, 896, 911
PteI GCGCGC 1 cut(s) 584
RsaI GTAC 2 cut(s) 612, 731
RsaNI GTAC 2 cut(s) 611, 730
SacI GAGCTC 1 cut(s) 234
SatI GCNGC 3 cut(s) 430, 699, 837
Sau3AI GATC 4 cut(s) 173, 870, 896, 911
Sau96I GGNCC 4 cut(s) 157, 440, 755, 885
SchI GAGTC 1 cut(s) 615
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 2 cut(s) 95, 710
SfcI CTRYAG 1 cut(s) 177
SfoI GGCGCC 1 cut(s) 397
SgrAI CRCCGGYG 1 cut(s) 398
SinI GGWCC 3 cut(s) 157, 440, 755
SmlI CTYRAG 2 cut(s) 136, 827
SmoI CTYRAG 2 cut(s) 136, 827
SnaBI TACGTA 1 cut(s) 861
Sse9I AATT 6 cut(s) 80, 256, 283, 561, 667, 788
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 2 cut(s) 222, 714
SspDI GGCGCC 1 cut(s) 395
SspMI CTAG 2 cut(s) 201, 900
SstI GAGCTC 1 cut(s) 234
StuI AGGCCT 1 cut(s) 274
StyI CCWWGG 3 cut(s) 117, 216, 235
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 1 cut(s) 863
TaqI TCGA 2 cut(s) 277, 671
TasI AATT 6 cut(s) 80, 256, 283, 561, 667, 788
TatI WGTACW 1 cut(s) 729
TfiI GAWTC 2 cut(s) 189, 693
TscAI CASTG 4 cut(s) 172, 424, 532, 550
TseFI GTSAC 2 cut(s) 239, 401
TseI GCWGC 3 cut(s) 429, 698, 836
Tsp45I GTSAC 2 cut(s) 239, 401
TspDTI ATGAA 4 cut(s) 181, 269, 573, 612
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 4 cut(s) 172, 424, 532, 550
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 3 cut(s) 157, 440, 755
XapI RAATTY 2 cut(s) 256, 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmiI GTMKAC 2 cut(s) 28, 651
XspI CTAG 2 cut(s) 201, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.