FvH4_2g20870

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013613
Physical Location & Seq
Reverse (-)
17425233 .. 17430159
4927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20870.t1

Sequence Viewer

Length: 2907 bp
ATGGAATCGTCTTCCACTTCAACCTCTCGCTTTCGCTTCCGAGACCTCCCAGAAATGGAGAACTCCACGCAGTCCGACACCGCCTCCGACGCTTCCTCCGTCGTCTCCACTGACCGCGATGACGACTCCAACCTCCAATCCATGACCGCTAGGGGAATTAAGCATCTTTGTTCGGAGCTACTCGAGCTGAAGGTTGCATCCAATGAAGATTTCCACAGTCACATCTTTGCTAATTATTCCTCCTTTGTTAGAGTCTTTGAAGAAGTAGGACGCATGGAGACTGAGCTGATGCAGCTGAAGAACCATGTTTCGAGTCAAAAGAGGCTTGTGAATGACCTTGTAGATGGCATATATTCGAAGGTTTTATCGAAGGAGAGTATGGACTTCGTTATTGAAGAATCTGAATGGGATGAGGCGCTTCCGATCGGTGAGTTAGAGGCTCATGCAAATGATGTTTTGGAAACTTTGGACGTTCTCTTGTGTGAGAATAGGATAGATGAAGCGCTGGCTATCTTAGACTTGGAGGATGTGAATTTTGAAAGGATGCAGTTTGAAACGGATTGTCCGGTGGAAGAGTTGAGATTATTTGAGTCTGCAGTTTCTGAGAGGAAGGGCTTGCTTACACAACGGTTGGCAATCGTGGCTGAAAATCCAAGAATATCTGCAGCCGAACTTCAGAAAGCACTGGTGGGGCTTTGCAGACTGGGTGATATTCATCTTGCAACTAGTTTATTGCTCAAATATTACCATTCACGCATTGCTTCTGGGATTAATAATTTGAAGGCTTCTAAATCGTTGTTGGATAGGGTATACATCAGGGAACTTTCAAAGTTCGTGTTTTCTATGATTTCTCAAGCAGCAAGAAGTTTTGTGATGTTATTTGGAGAAACTTCTCCTTATGCACCAGAAGTTATTAAGTGGACTCGTGAAGAAACCAAAGGTTTTGCCGCTTGTTTTGATAAGTATGTCAAGTCCATCTCAGAAGTAAGTGGTGGATTGTCCACAGCAGTAGAAGCTGTGCAGTTTGTGATTTCATTTTGTTCTTTGCTGGAAACTCAGAGATTAGTGTTGCGGCCATACTTGATCAAGCATATTCGCCCTTGCATGGAAGATGTTCTAAATATACACCTGGATCATTTTACAAAAGTCATTGGAATCTTTACAGCAACTGATACATGGGTTTTGGGTAGATATCTTGTATCTGGAATTATGAATGAACGGTGCTCTTCCATGGTTGTTGGGCAACAGCCAGAATATTGCTTGCTCACTAACAGCGGCCGGAAGTTTGTAACACTGCTGCAGGCAATAACTGTAGATGTCAGCCCATTACTTGCCCTTGAAATTGAAGGATCAATTCTTAGTGGACTTATGAACCTCTTCAAAAAGTATATAGACATCCTTCAAAGAGCCATCAACTGTGACACAACTGTTACAGAAAAAATCAGTTCGAAGATCAATTTGGCCGAGTCAGTGCAACAACAGGTATCTATCCTAGCCAATCTATCAGCACTTAAGCAACTTTTCTCCAACATGGTCAGGAGTATTTTCAAGGGTGCTAGTGATACCATTTCTGAAATAAGAAAAAGCCACCCCGATGGTTATCAACATAAGGAATTTGACAGTTGTATATTGTTTATTCAAGAAGCCTCTCTTGGGCTCAGATCTCTATTCTGCGAGCAATTCATATATAGAGTAATGTCCCTCAAAACTAGCTACAAACTTCCAGAAATCCGCATTAACAGCCATGGCGAGTCCTGCATGTTACATGGAGGAATGCCATCCCTTCCCTTTCAGGTACTGTTTCTAGAACTGAGGAAACTAGAAAACCTTTCCGAGGACAATGTTTTCGAAGTTGAATGGTTGATAAAGCTAATAGGGGATCTTATAGAGGCTGTTTTCGTTTGGATTTCAAATAACAAAGAGTTCTGGGATACTAATGTGGATATGACCGAAGATCATCCTCTCGCTTTCAAACAGTTTGTTTTGGATGTGCAATTTCTTGTGGAAATCACGAAGTACGGAGGGTACTTCTCCAACAATCCTTTTTTTCTCATGAATCTCATGAAATCAGCCTTTTTTTCAGCTGGACTAAATCCTAGAAGAGATGTCAACGACGATGCATGGGCTATAGATGCTGCTGCTGAAACAATCCAAAAGTTGTTGGAGATTGAGAAGGAACAAGAGCATGTCAGTGTAGAAGAAGCAGAGGAACAACCCAATAATGTGGCCGACTCTTTTCAGGAGGATGCTAGAAGCTGTCTAGAGGGTTCTGCGAAATCAGAGGAGCATGAAGTAGCCTCATATGACACAGAAGTTTCCATTAATGCTGAATCAGCGTCTTTGAATAGATCTCACTCTTTTGAGGAAGATGTTACAAGTTCTTTAGAGGAGGATTATGTGAAACTAGAGGAGGGTGCAATAGCCTCATATGATCAACAAGTTGACTTCAATGCTGAAACAACCTCGTCACTGGAAGCTGAACCGAGTGAATCAAAGTATGCTACTGACAATTCTGAGAATGATGCTGCAAATCCTTCAGAAGATCATATGCAATTAGAGGAGGACATGACAACCATATACAATCCACAATTTAATGCAGAAACAGTATCACCGAGTACAGAATCTTGTGCTCTTGGATTATCTGCCAATTTTTCAGATGTGGATGGAAATTACAGTGACGGAACTGCCACAATTGTGACTGATACCTCCTTAGAGTCTCCAAATACTGGTCTGGGAAGTGAATCCAACAAGCCAGTTGATGTGTTAGAGACCATTGAAGAAACAAATGAATCAACAGTAGTGTTGCCTATGCATAACATGGATGAGGCCTCCAAGATGAAGGTCCCTGATGAGGAGGGAACTCGCATTCAGTCTGAACCCTCCGAGCAGGTATTGAACAATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo84_C Vps51

Protein Analysis

969

Amino Acids

108.57

Weight (kDa)

4.54

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo84_C PF16528 150 - 330 1.9e-11 Exocyst component 84 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2878
AccB7I CCANNNNNTGG 1 cut(s) 2726
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 117
AciI CCGC 7 cut(s) 81, 115, 147, 948, 1072, 1275, 1732
AclWI GGATC 3 cut(s) 1140, 1357, 1887
AcoI YGGCCR 4 cut(s) 1073, 1276, 1461, 2226
AcsI RAATTY 2 cut(s) 532, 1613
AcuI CTGAAG 4 cut(s) 209, 317, 659, 2520
AfaI GTAC 4 cut(s) 1797, 2018, 2027, 2617
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 6 cut(s) 55, 1105, 1653, 1834, 2726, 2851
AflII CTTAAG 1 cut(s) 1511
AhlI ACTAGT 1 cut(s) 725
AjnI CCWGG 1 cut(s) 1128
AjuI GAANNNNNNNTTGG 6 cut(s) 194, 226, 1442, 1474, 1635, 1667
AleI CACNNNNGTG 1 cut(s) 2693
Alw21I GWGCWC 2 cut(s) 1226, 2632
Alw26I GTCTC 5 cut(s) 36, 109, 272, 2721, 2762
AlwI GGATC 3 cut(s) 1140, 1357, 1887
AlwNI CAGNNNCTG 3 cut(s) 602, 1169, 1798
Ama87I CYCGRG 1 cut(s) 182
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 5 cut(s) 1073, 1276, 1461, 2226, 2826
ApeKI GCWGC 7 cut(s) 292, 665, 857, 1297, 2135, 2138, 2525
ApoI RAATTY 2 cut(s) 532, 1613
ArsI GACNNNNNNTTYG 2 cut(s) 1829, 1861
AseI ATTAAT 2 cut(s) 771, 2322
Asp700I GAANNNNTTC 2 cut(s) 1113, 1376
AspLEI GCGC 2 cut(s) 418, 505
AspS9I GGNCC 1 cut(s) 2842
AsuHPI GGTGA 3 cut(s) 440, 719, 2601
AsuII TTCGAA 3 cut(s) 356, 1448, 1848
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 2842
BaeI ACNNNNGTAYC 4 cut(s) 1164, 1197, 1923, 1956
BanII GRGCYC 1 cut(s) 1659
BauI CACGAG 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 2 cut(s) 1226, 2632
BbvI GCAGC 7 cut(s) 304, 677, 869, 1284, 2122, 2125, 2512
BccI CCATC 6 cut(s) 338, 983, 1418, 1589, 1786, 2657
BciT130I CCWGG 1 cut(s) 1130
BciVI GTATCC 1 cut(s) 1924
BclI TGATCA 2 cut(s) 1083, 2431
BcoDI GTCTC 5 cut(s) 36, 109, 272, 2721, 2762
BcuI ACTAGT 1 cut(s) 725
BfmI CTRYAG 5 cut(s) 594, 663, 1298, 1311, 2127
BfoI RGCGCY 2 cut(s) 419, 506
BfrI CTTAAG 1 cut(s) 1511
BfuAI ACCTGC 1 cut(s) 2878
BfuI GTATCC 1 cut(s) 1924
BglII AGATCT 2 cut(s) 1661, 2348
Bme1390I CCNGG 1 cut(s) 1130
Bme18I GGWCC 1 cut(s) 2842
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 2842
BmiI GGNNCC 1 cut(s) 2844
BmrFI CCNGG 1 cut(s) 1130
BmrI ACTGGG 1 cut(s) 713
BmsI GCATC 8 cut(s) 172, 206, 279, 534, 2107, 2122, 2236, 2512
BmuI ACTGGG 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 3
Bpu14I TTCGAA 3 cut(s) 356, 1448, 1848
BpuEI CTTGAG 1 cut(s) 837
BsaBI GATNNNNATC 2 cut(s) 714, 1599
BsaI GGTCTC 2 cut(s) 36, 2762
BsaJI CCNNGG 3 cut(s) 1230, 1744, 1833
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 4 cut(s) 68, 80, 98, 110
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 1105, 1653, 1834, 2726, 2851
Bse1I ACTGG 5 cut(s) 690, 708, 2475, 2731, 2753
Bse3DI GCAATG 1 cut(s) 756
Bse8I GATNNNNATC 2 cut(s) 714, 1599
BseBI CCWGG 1 cut(s) 1130
BseDI CCNNGG 3 cut(s) 1230, 1744, 1833
BseJI GATNNNNATC 2 cut(s) 714, 1599
BseLI CCNNNNNNNGG 6 cut(s) 55, 1105, 1653, 1834, 2726, 2851
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 7 cut(s) 273, 594, 993, 1070, 1672, 1802, 2505
BseNI ACTGG 5 cut(s) 690, 708, 2475, 2731, 2753
BseRI GAGGAG 5 cut(s) 2297, 2402, 2423, 2573, 2867
BseX3I CGGCCG 1 cut(s) 1276
BseXI GCAGC 7 cut(s) 304, 677, 869, 1284, 2122, 2125, 2512
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 2 cut(s) 426, 1279
BshFI GGCC 5 cut(s) 1075, 1278, 1463, 2228, 2828
BsiEI CGRYCG 2 cut(s) 426, 1279
BsiHKAI GWGCWC 2 cut(s) 1226, 2632
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 566, 1279
BslFI GGGAC 2 cut(s) 1684, 2828
BslI CCNNNNNNNGG 6 cut(s) 55, 1105, 1653, 1834, 2726, 2851
BsmAI GTCTC 5 cut(s) 36, 109, 272, 2721, 2762
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 1684, 2828
BsmI GAATGC 2 cut(s) 1779, 2865
BsnI GGCC 5 cut(s) 1075, 1278, 1463, 2228, 2828
Bso31I GGTCTC 2 cut(s) 36, 2762
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 3 cut(s) 356, 1448, 1848
Bsp1286I GDGCHC 3 cut(s) 1226, 1659, 2632
Bsp19I CCATGG 2 cut(s) 1230, 1744
BspACI CCGC 7 cut(s) 81, 115, 147, 948, 1072, 1275, 1732
BspANI GGCC 5 cut(s) 1075, 1278, 1463, 2228, 2828
BspCNI CTCAG 7 cut(s) 274, 595, 992, 1069, 1671, 1803, 2506
BspFNI CGCG 1 cut(s) 117
BspHI TCATGA 2 cut(s) 2052, 2061
BspLI GGNNCC 1 cut(s) 2844
BspMAI CTGCAG 3 cut(s) 598, 667, 1302
BspMI ACCTGC 1 cut(s) 2878
BspPI GGATC 3 cut(s) 1140, 1357, 1887
BspQI GCTCTTC 1 cut(s) 1231
BspT104I TTCGAA 3 cut(s) 356, 1448, 1848
BspTI CTTAAG 1 cut(s) 1511
BspTNI GGTCTC 2 cut(s) 36, 2762
BsrDI GCAATG 1 cut(s) 756
BsrI ACTGG 5 cut(s) 690, 708, 2475, 2731, 2753
BssECI CCNNGG 3 cut(s) 1230, 1744, 1833
BssNAI GTATAC 1 cut(s) 811
BssSI CACGAG 1 cut(s) 924
BssT1I CCWWGG 2 cut(s) 1230, 1744
Bst1107I GTATAC 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 924
Bst2UI CCWGG 1 cut(s) 1130
Bst6I CTCTTC 4 cut(s) 567, 1231, 1382, 2095
BstAFI CTTAAG 1 cut(s) 1511
BstBI TTCGAA 3 cut(s) 356, 1448, 1848
BstC8I GCNNGC 5 cut(s) 507, 617, 1262, 1302, 1676
BstDSI CCRYGG 2 cut(s) 1230, 1744
BstENI CCTNNNNNAGG 1 cut(s) 1832
BstFNI CGCG 1 cut(s) 117
BstH2I RGCGCY 2 cut(s) 419, 506
BstHHI GCGC 2 cut(s) 418, 505
BstMAI GTCTC 5 cut(s) 36, 109, 272, 2721, 2762
BstMCI CGRYCG 2 cut(s) 426, 1279
BstMWI GCNNNNNNNGC 9 cut(s) 89, 184, 292, 641, 1013, 1740, 1755, 2132, 2333
BstNI CCWGG 1 cut(s) 1130
BstNSI RCATGY 2 cut(s) 1762, 2189
BstSCI CCNGG 1 cut(s) 1128
BstSFI CTRYAG 5 cut(s) 594, 663, 1298, 1311, 2127
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 7 cut(s) 304, 677, 869, 1284, 2122, 2125, 2512
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 3 cut(s) 1661, 1879, 2348
BstXI CCANNNNNNTGG 2 cut(s) 1595, 2224
BstYI RGATCY 3 cut(s) 1661, 1879, 2348
BstZ17I GTATAC 1 cut(s) 811
BstZI CGGCCG 1 cut(s) 1276
BsuI GTATCC 1 cut(s) 1924
BsuRI GGCC 5 cut(s) 1075, 1278, 1463, 2228, 2828
BtgI CCRYGG 2 cut(s) 1230, 1744
BtgZI GCGATG 1 cut(s) 132
BtsI GCAGTG 1 cut(s) 1292
BtsIMutI CAGTG 7 cut(s) 108, 683, 1292, 1476, 2197, 2468, 2680
BveI ACCTGC 1 cut(s) 2878
Cac8I GCNNGC 5 cut(s) 507, 617, 1262, 1302, 1676
CaiI CAGNNNCTG 3 cut(s) 602, 1169, 1798
CciI TCATGA 2 cut(s) 2052, 2061
CfoI GCGC 2 cut(s) 418, 505
Cfr13I GGNCC 1 cut(s) 2842
CseI GACGC 3 cut(s) 98, 279, 2325
Csp6I GTAC 4 cut(s) 1796, 2017, 2026, 2616
CviQI GTAC 4 cut(s) 1796, 2017, 2026, 2616
EaeI YGGCCR 4 cut(s) 1073, 1276, 1461, 2226
EagI CGGCCG 1 cut(s) 1276
Eam1104I CTCTTC 4 cut(s) 567, 1231, 1382, 2095
EarI CTCTTC 4 cut(s) 567, 1231, 1382, 2095
EclXI CGGCCG 1 cut(s) 1276
Eco130I CCWWGG 2 cut(s) 1230, 1744
Eco147I AGGCCT 1 cut(s) 2828
Eco24I GRGCYC 1 cut(s) 1659
Eco31I GGTCTC 2 cut(s) 36, 2762
Eco32I GATATC 1 cut(s) 1193
Eco47I GGWCC 1 cut(s) 2842
Eco47III AGCGCT 1 cut(s) 504
Eco52I CGGCCG 1 cut(s) 1276
Eco57I CTGAAG 4 cut(s) 209, 317, 659, 2520
Eco88I CYCGRG 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 1832
EcoO109I RGGNCCY 1 cut(s) 2842
EcoRII CCWGG 1 cut(s) 1128
EcoRV GATATC 1 cut(s) 1193
EcoT14I CCWWGG 2 cut(s) 1230, 1744
EcoT22I ATGCAT 2 cut(s) 2122, 2814
EcoT38I GRGCYC 1 cut(s) 1659
ErhI CCWWGG 2 cut(s) 1230, 1744
Esp3I CGTCTC 1 cut(s) 109
FalI AAGNNNNNCTT 2 cut(s) 1635, 1667
FaqI GGGAC 2 cut(s) 1684, 2828
FauNDI CATATG 3 cut(s) 2302, 2428, 2547
FbaI TGATCA 2 cut(s) 1083, 2431
FblI GTMKAC 1 cut(s) 810
FriOI GRGCYC 1 cut(s) 1659
GlaI GCGC 2 cut(s) 417, 504
HaeII RGCGCY 2 cut(s) 419, 506
HaeIII GGCC 5 cut(s) 1075, 1278, 1463, 2228, 2828
HapII CCGG 2 cut(s) 566, 1279
HgaI GACGC 3 cut(s) 98, 279, 2325
HhaI GCGC 2 cut(s) 418, 505
Hin6I GCGC 2 cut(s) 416, 503
HinP1I GCGC 2 cut(s) 416, 503
HincII GTYRAC 2 cut(s) 2110, 2443
HindII GTYRAC 2 cut(s) 2110, 2443
HpaII CCGG 2 cut(s) 566, 1279
HphI GGTGA 3 cut(s) 440, 719, 2601
Hpy166II GTNNAC 6 cut(s) 811, 921, 1002, 1364, 2110, 2443
Hpy8I GTNNAC 6 cut(s) 811, 921, 1002, 1364, 2110, 2443
Hpy99I CGWCG 3 cut(s) 92, 104, 2117
HpyCH4IV ACGT 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 184, 292, 641, 1013, 1740, 1755, 2132, 2333
HpySE526I ACGT 1 cut(s) 471
HspAI GCGC 2 cut(s) 416, 503
Ksp22I TGATCA 2 cut(s) 1083, 2431
LguI GCTCTTC 1 cut(s) 1231
LmnI GCTCC 2 cut(s) 175, 2284
Lsp1109I GCAGC 7 cut(s) 304, 677, 869, 1284, 2122, 2125, 2512
LweI GCATC 8 cut(s) 172, 206, 279, 534, 2107, 2122, 2236, 2512
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 9 cut(s) 218, 1288, 1418, 1429, 1761, 2371, 2466, 2675, 2695
MfeI CAATTG 1 cut(s) 2691
MflI RGATCY 3 cut(s) 1661, 1879, 2348
MhlI GDGCHC 3 cut(s) 1226, 1659, 2632
MlyI GAGTC 9 cut(s) 119, 261, 322, 599, 916, 1475, 1760, 2225, 2723
MmeI TCCRAC 8 cut(s) 99, 111, 153, 780, 1551, 2058, 2142, 2769
Mph1103I ATGCAT 2 cut(s) 2122, 2814
MroXI GAANNNNTTC 2 cut(s) 1113, 1376
MseI TTAA 7 cut(s) 159, 771, 915, 1512, 1737, 2322, 2592
MslI CAYNNNNRTG 4 cut(s) 447, 1593, 2190, 2693
MspA1I CMGCKG 3 cut(s) 295, 1275, 2084
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 2 cut(s) 566, 1279
MspR9I CCNGG 1 cut(s) 1130
MunI CAATTG 1 cut(s) 2691
Mva1269I GAATGC 2 cut(s) 1779, 2865
MvaI CCWGG 1 cut(s) 1130
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 9 cut(s) 89, 184, 292, 641, 1013, 1740, 1755, 2132, 2333
NcoI CCATGG 2 cut(s) 1230, 1744
NdeI CATATG 3 cut(s) 2302, 2428, 2547
NlaIV GGNNCC 1 cut(s) 2844
NmeAIII GCCGAG 1 cut(s) 1489
NmuCI GTSAC 5 cut(s) 218, 1418, 2466, 2675, 2695
NsiI ATGCAT 2 cut(s) 2122, 2814
NspI RCATGY 2 cut(s) 1762, 2189
NspV TTCGAA 3 cut(s) 356, 1448, 1848
OliI CACNNNNGTG 1 cut(s) 2693
PaeR7I CTCGAG 1 cut(s) 182
PagI TCATGA 2 cut(s) 2052, 2061
PceI AGGCCT 1 cut(s) 2828
PciSI GCTCTTC 1 cut(s) 1231
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 2 cut(s) 1779, 2865
PdmI GAANNNNTTC 2 cut(s) 1113, 1376
PfeI GAWTC 9 cut(s) 5, 398, 1155, 2056, 2330, 2489, 2621, 2741, 2789
PflMI CCANNNNNTGG 1 cut(s) 2726
Ple19I CGATCG 1 cut(s) 426
PleI GAGTC 9 cut(s) 119, 260, 321, 598, 916, 1474, 1759, 2225, 2722
PpsI GAGTC 9 cut(s) 119, 260, 321, 598, 916, 1474, 1759, 2225, 2722
PpuMI RGGWCCY 1 cut(s) 2842
PshBI ATTAAT 2 cut(s) 771, 2322
Psp5II RGGWCCY 1 cut(s) 2842
Psp6I CCWGG 1 cut(s) 1128
PspGI CCWGG 1 cut(s) 1128
PspN4I GGNNCC 1 cut(s) 2844
PspPI GGNCC 1 cut(s) 2842
PspPPI RGGWCCY 1 cut(s) 2842
PspXI VCTCGAGB 1 cut(s) 182
PstI CTGCAG 3 cut(s) 598, 667, 1302
PstNI CAGNNNCTG 3 cut(s) 602, 1169, 1798
PsuI RGATCY 3 cut(s) 1661, 1879, 2348
PvuI CGATCG 1 cut(s) 426
PvuII CAGCTG 2 cut(s) 295, 2084
RsaI GTAC 4 cut(s) 1797, 2018, 2027, 2617
RsaNI GTAC 4 cut(s) 1796, 2017, 2026, 2616
RseI CAYNNNNRTG 4 cut(s) 447, 1593, 2190, 2693
SapI GCTCTTC 1 cut(s) 1231
SaqAI TTAA 7 cut(s) 159, 771, 915, 1512, 1737, 2322, 2592
Sau96I GGNCC 1 cut(s) 2842
SchI GAGTC 9 cut(s) 119, 261, 322, 599, 916, 1475, 1760, 2225, 2723
ScrFI CCNGG 1 cut(s) 1130
SduI GDGCHC 3 cut(s) 1226, 1659, 2632
SfaNI GCATC 8 cut(s) 172, 206, 279, 534, 2107, 2122, 2236, 2512
SfcI CTRYAG 5 cut(s) 594, 663, 1298, 1311, 2127
Sfr274I CTCGAG 1 cut(s) 182
SfuI TTCGAA 3 cut(s) 356, 1448, 1848
SinI GGWCC 1 cut(s) 2842
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 4 cut(s) 447, 1593, 2190, 2693
SmlI CTYRAG 3 cut(s) 182, 852, 1511
SmoI CTYRAG 3 cut(s) 182, 852, 1511
SpeI ACTAGT 1 cut(s) 725
SseBI AGGCCT 1 cut(s) 2828
SsiI CCGC 7 cut(s) 81, 115, 147, 948, 1072, 1275, 1732
SspI AATATT 2 cut(s) 743, 1256
StuI AGGCCT 1 cut(s) 2828
StyD4I CCNGG 1 cut(s) 1128
StyI CCWWGG 2 cut(s) 1230, 1744
TaiI ACGT 1 cut(s) 474
TaqI TCGA 6 cut(s) 183, 311, 356, 368, 1448, 1848
TaqII GACCGA 1 cut(s) 1964
TatI WGTACW 1 cut(s) 2615
TauI GCSGC 3 cut(s) 950, 1075, 1278
TfiI GAWTC 9 cut(s) 5, 398, 1155, 2056, 2330, 2489, 2621, 2741, 2789
Tru1I TTAA 7 cut(s) 159, 771, 915, 1512, 1737, 2322, 2592
Tru9I TTAA 7 cut(s) 159, 771, 915, 1512, 1737, 2322, 2592
TscAI CASTG 7 cut(s) 115, 690, 1299, 1476, 2197, 2475, 2680
TseFI GTSAC 5 cut(s) 218, 1418, 2466, 2675, 2695
TseI GCWGC 7 cut(s) 292, 665, 857, 1297, 2135, 2138, 2525
Tsp45I GTSAC 5 cut(s) 218, 1418, 2466, 2675, 2695
TspGWI ACGGA 4 cut(s) 88, 572, 2034, 2694
TspRI CASTG 7 cut(s) 115, 690, 1299, 1476, 2197, 2475, 2680
Van91I CCANNNNNTGG 1 cut(s) 2726
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 1 cut(s) 2842
VspI ATTAAT 2 cut(s) 771, 2322
XagI CCTNNNNNAGG 1 cut(s) 1832
XapI RAATTY 2 cut(s) 532, 1613
XbaI TCTAGA 2 cut(s) 1804, 2260
XceI RCATGY 2 cut(s) 1762, 2189
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 2 cut(s) 1113, 1376
Zsp2I ATGCAT 2 cut(s) 2122, 2814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.