MD05G1192300.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013613
Physical Location & Seq
Reverse (-)
32046960 .. 32051716
4757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1192300.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGGAATCTTCTTCCACTTCTTCCAGATTCCGATTCCGGGACCTGCCGGAAATGGAGAACTCCACGCAGTCCGACACCGCCTCTGACTTGTCCTCCGTGTCCAGCGACGGAGAAGACGACGAGTCCGACCTTCAATCCATGACTGGAAAGGGAATTAAGCATCTGTGTTCAGAACTCTTGGAGCTAAAGGCTGCATCCGGCGAAGAATTTCACAAACACATCTTTTCGAATTATTCGGCCTTCGTCAGAATATTTGAAGAAGTAGGGCTTGTGGAAAATGAGTTGAAGCAACTGAAGAACCATGTAGTGACCCAAAAGAGGCTTGTAAGTGAACTTGCAGATGGCATATATTTGAAGGTTTTATCAAAGGAGACTATAGACTTGGTTTTTGAAGAACCCGAATTTTCGGAATTTTCAGAACCAGTTACCATCACCAACTTAGGGGATCATGCAAATGATGTTTTAGCAACCTTGGACACTCTCTTGTCTGAAAACAGAATCGATGAAGCATTGACCATCTTCGAATTAGAGGACGAGAATTTCCAGAGGTTGCAGTTTGAGGAGGGTCGCCCGTTGGATGAGCTGAGGTTATTTAGTACTGAAATTTCCGAGAGGAAGGCTTTGGTTACAATGCAGTTGACAATGGTGGCTGAAAATCCAAGAATAGCTGCGCCGGAACTTCAGAAAGCGCTGGTGGGGCTCTGCAGACTAGGTGACAGCCATCTCGCAACACAATTGTTGATCAAGTATTACCGTTCGCGCATTGCGTCTGGGACACTAAATTTGCAGGCTTCGAAATCGTTTTTGAATAGGGTTTATATCAGGGAGCTTTCAAAGTTTGTGTTCTCTATGATTTCTCAAGCAGCAAGAAGTTTTGTGATGTTATATGGGGAGACTTCTTCTTATGCATCAGAACTTATCCAGTGGGCTCGTAGAGAAACAAAATTGTTTGGTGCTTGTTTCGACAGGTATGTGAAATCCATATCAGAAATAAGTGGTGGATTGTCCACAGCAGTAGAAGCTGTGCAGTTTGCAATGTCTTTTTGCTCTTTGTTGGAAAGTCAGAGATTAGTGTTGCGGCCGTACTTGATCAACTACATTCGCCCTTGTATCGAAGAGGTTCTGCGTAAACACCTAGACCATTTTAAGAAAGTCGTCGATATCTTTACAGCAACTGATGCTTGGGTTTTGGGAAGATATCTTGTATCTGGAATCATGAATGAAGGGTGCTCTTCCATGGTTGTTGGCCAACAACCAGAATATTGCTTGCTCACAAACAGCGGCAGGAAGTTTGTAACGCTGCTGCAGTCTATCACTTTAGATGTCACCCCATTAGTTTCCCTTCAAATCGAAGGATTAATCGTTAGCGGACTTATGAATCTCTTTAAAAAGTATGTAGACATCCTTGCGATAGCCATCAACTGTCAGGTGACTGTTAAAGAAAAAAGTAATCCAAGAATATACTTGGCCGAGTCAGTGCAGCAACAGGTTTCAATACTAGCCAATCTGTCATCGCTCGAGCAACTCTTCTCCAACATGGTTAGAAGTATCTTCAAGGCTGTCAGTGACATCAACTCTGAGTTAATGAAAACCCTTCCAGACAGTTATCAAGCGAAGGAACTTGATAGTTGTATCTCATTGATTCAGGAGGCTTCTAGTCGTCTCAGAGATCTTTATTGTGAGCAATTTATATGTAAAATTATGTCCCTAAGAAATAGTGACAACCTTACTCCCGAAAATTGCATTGACGACCAGGGTGAGTCTTGCGTGTTCCAGGGATTGATGCCCTCTCTTGCTTCTCAGGTACTGTTTTTAGAACTTCGGAAACTGGAAAAGCTTTCTGAAGACAATGTCTTTGAAATTGATTGGTTAATGGAGCTACTAAGGGAGCTCATAGAGGCCGTATTTGCTTGGATTCCAAACAACATAGAGATCTGGGATACCGATAGCGAGAATATGATGGAAGAGCATCCTTTCAACTCGAAGCAGTTTGTTTTGGACGTGCAATTTCTGGTGGAAATCTCAGCGTATGGAGGATACTTCTCCAACAACCCTTTGTTTCTCATAAATCTCATGAAATCGGCCTTGCTTTCAGCTGGACTAGATCCAAAAAGAGATGTCAGCGAGGATGTATGGGCTATAGACTCTGCTGCCGAAGCAATCCAAAAGCTGCTGGAGATTGAGAATTCACAGTCACGTGTGGACCAAGAGCCTCTCTCTGAACTAGAATCAGGGGAGGAGGAACCATGCAAGTTCGCCACTGAATCTTTCCAGGATGATCGTAGTTCTTCAGAGGAGGATGCAGTAGTCACAGATGATTCAGAAGGCGTCTCCAATGCAGAATTGGAACCATTGAATGCAGAAGAACCATGCATGGAGAAGTTCAAGATTTCCACCGACTCTGTTCAGGATGATGCTTGTTCTTCAAATGAGAGTTTAGTAGCCACATATGGTTCGGAAGGTGCGCTTAATGCTGAATCAGAACCATTGGATACAGAACCATCTGGGGACCAATCCAAGAATCCGGCTAAGTCTTTTGCGGACAATGTTATAAGCTCTTTAGAAGATTATGCAGAGTCAGAGGAGGACGAAATGGCCACATATGATCCTGGATGCATGGAGAAGTCCGAAAATGCATCTGAATCTTTTCAGGAAGATGTTACAAGTTCTTTGGATGATTCTGTGGAACCGGAAGTTGCCATTAATGCTGAAACAGAATCGTTTGCTGAACCACGCACTGTTGAATCACAGCGCGTAACTGAGTCTTTCGAGGATGATGCTTTAAGTCCTTCAGAGGATGATGTGGTAGCCATGGACAAAGCTGAAGCTGCCTCTAGCGCAGAAACAACACCATTGATTAAAGAACCATGTCCTCAAACTAAGGTCTTCGAAAAAGCGTCTGACAATGCAGTTGATACGTTACAGATCATTGAAGAAACTAATGAACCATCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo84_C Vps51

Protein Analysis

986

Amino Acids

110.12

Weight (kDa)

4.33

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo84_C PF16528 153 - 332 1.2e-10 Exocyst component 84 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2552
Acc36I ACCTGC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 1398
AccII CGCG 2 cut(s) 760, 2754
AciI CCGC 5 cut(s) 78, 1078, 1281, 1368, 2540
AclWI GGATC 3 cut(s) 453, 2099, 2600
AcoI YGGCCR 4 cut(s) 1079, 1246, 1467, 2595
AcsI RAATTY 7 cut(s) 206, 401, 410, 538, 603, 781, 2185
AcuI CTGAAG 6 cut(s) 314, 665, 1863, 2274, 2775, 2844
AcvI CACGTG 1 cut(s) 2198
AcyI GRCGYC 1 cut(s) 2328
AfaI GTAC 3 cut(s) 598, 1085, 1806
AfeI AGCGCT 1 cut(s) 690
AfiI CCNNNNNNNGG 3 cut(s) 37, 318, 441
AflIII ACRYGT 1 cut(s) 2197
AhdI GACNNNNNGTC 1 cut(s) 121
AjiI CACGTC 1 cut(s) 2002
AjnI CCWGG 4 cut(s) 1752, 1773, 2271, 2608
Alw21I GWGCWC 2 cut(s) 1232, 1893
Alw26I GTCTC 4 cut(s) 365, 887, 1667, 2335
AlwI GGATC 3 cut(s) 453, 2099, 2600
AlwNI CAGNNNCTG 2 cut(s) 1175, 1807
Ama87I CYCGRG 1 cut(s) 1517
Aor51HI AGCGCT 1 cut(s) 690
AoxI GGCC 7 cut(s) 237, 1079, 1246, 1467, 1899, 2082, 2595
ApeKI GCWGC 9 cut(s) 191, 668, 863, 1300, 1303, 1480, 2150, 2170, 2828
ApoI RAATTY 7 cut(s) 206, 401, 410, 538, 603, 781, 2185
ArsI GACNNNNNNTTYG 6 cut(s) 371, 403, 766, 798, 1838, 1870
AseI ATTAAT 2 cut(s) 1358, 2703
Asp700I GAANNNNTTC 3 cut(s) 207, 1119, 2646
AspLEI GCGC 6 cut(s) 673, 691, 762, 2467, 2754, 2840
AspS9I GGNCC 3 cut(s) 40, 2203, 2509
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 5 cut(s) 424, 725, 1318, 1441, 1769
AsuII TTCGAA 4 cut(s) 227, 522, 794, 2889
AvaI CYCGRG 1 cut(s) 1517
AvaII GGWCC 3 cut(s) 40, 2203, 2509
BalI TGGCCA 2 cut(s) 1248, 2597
BanII GRGCYC 3 cut(s) 702, 931, 1893
BbrPI CACGTG 1 cut(s) 2198
BbsI GAAGAC 3 cut(s) 120, 1851, 2878
Bbv12I GWGCWC 2 cut(s) 1232, 1893
BbvCI CCTCAGC 1 cut(s) 584
BbvI GCAGC 9 cut(s) 178, 655, 875, 1287, 1290, 1492, 2137, 2157, 2815
BccI CCATC 8 cut(s) 335, 437, 524, 729, 1424, 1954, 2509, 2956
BceAI ACGGC 2 cut(s) 1066, 1886
BcgI CGANNNNNNTGC 2 cut(s) 2759, 2793
BciT130I CCWGG 4 cut(s) 1754, 1775, 2273, 2610
BciVI GTATCC 3 cut(s) 1933, 2030, 2485
BclI TGATCA 2 cut(s) 741, 1089
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 4 cut(s) 365, 887, 1667, 2335
BfaI CTAG 7 cut(s) 710, 1136, 1499, 1656, 2102, 2225, 2835
BfmI CTRYAG 4 cut(s) 375, 703, 1304, 2139
BfoI RGCGCY 1 cut(s) 692
BfuAI ACCTGC 1 cut(s) 51
BfuI GTATCC 3 cut(s) 1933, 2030, 2485
BglII AGATCT 2 cut(s) 1669, 1932
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 5 cut(s) 38, 1754, 1775, 2273, 2610
Bme18I GGWCC 3 cut(s) 40, 2203, 2509
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 1517
BmgBI CACGTC 1 cut(s) 2002
BmgT120I GGNCC 3 cut(s) 40, 2203, 2509
BmiI GGNNCC 5 cut(s) 41, 2244, 2349, 2510, 2688
BmrFI CCNGG 5 cut(s) 38, 1754, 1775, 2273, 2610
BpiI GAAGAC 3 cut(s) 120, 1851, 2878
BplI GAGNNNNNCTC 2 cut(s) 2201, 2233
BpmI CTGGAG 1 cut(s) 2195
Bpu10I CCTNAGC 1 cut(s) 584
Bpu14I TTCGAA 4 cut(s) 227, 522, 794, 2889
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 501
BsaAI YACGTR 1 cut(s) 2198
BsaBI GATNNNNATC 1 cut(s) 1415
BsaHI GRCGYC 1 cut(s) 2328
BsaJI CCNNGG 5 cut(s) 471, 1236, 1753, 1774, 2811
BsaWI WCCGGW 1 cut(s) 2689
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 318, 441
Bse1I ACTGG 4 cut(s) 148, 422, 922, 1833
Bse3DI GCAATG 2 cut(s) 762, 1041
Bse8I GATNNNNATC 1 cut(s) 1415
BseBI CCWGG 4 cut(s) 1754, 1775, 2273, 2610
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 5 cut(s) 471, 1236, 1753, 1774, 2811
BseJI GATNNNNATC 1 cut(s) 1415
BseLI CCNNNNNNNGG 3 cut(s) 37, 318, 441
BseMI GCAATG 2 cut(s) 762, 1041
BseMII CTCAG 6 cut(s) 575, 1569, 1678, 1814, 2037, 2751
BseNI ACTGG 4 cut(s) 148, 422, 922, 1833
BseRI GAGGAG 4 cut(s) 575, 2252, 2309, 2597
BseX3I CGGCCG 1 cut(s) 1079
BseXI GCAGC 9 cut(s) 178, 655, 875, 1287, 1290, 1492, 2137, 2157, 2815
BsgI GTGCAG 2 cut(s) 1046, 1499
Bsh1236I CGCG 2 cut(s) 760, 2754
Bsh1285I CGRYCG 1 cut(s) 1082
BshFI GGCC 7 cut(s) 239, 1081, 1248, 1469, 1901, 2084, 2597
BshVI ATCGAT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 1082
BsiHKAI GWGCWC 2 cut(s) 1232, 1893
BsiHKCI CYCGRG 1 cut(s) 1517
BsiSI CCGG 6 cut(s) 37, 47, 198, 674, 2525, 2690
BslFI GGGAC 4 cut(s) 53, 787, 1690, 2522
BslI CCNNNNNNNGG 3 cut(s) 37, 318, 441
BsmAI GTCTC 4 cut(s) 365, 887, 1667, 2335
BsmBI CGTCTC 2 cut(s) 1667, 2335
BsmFI GGGAC 4 cut(s) 53, 787, 1690, 2522
BsmI GAATGC 1 cut(s) 2362
BsnI GGCC 7 cut(s) 239, 1081, 1248, 1469, 1901, 2084, 2597
BsoBI CYCGRG 1 cut(s) 1517
Bsp119I TTCGAA 4 cut(s) 227, 522, 794, 2889
Bsp1286I GDGCHC 4 cut(s) 702, 931, 1232, 1893
Bsp143I GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
Bsp19I CCATGG 2 cut(s) 1236, 2811
BspACI CCGC 5 cut(s) 78, 1078, 1281, 1368, 2540
BspANI GGCC 7 cut(s) 239, 1081, 1248, 1469, 1901, 2084, 2597
BspCNI CTCAG 6 cut(s) 576, 1570, 1677, 1813, 2036, 2752
BspDI ATCGAT 1 cut(s) 501
BspFNI CGCG 2 cut(s) 760, 2754
BspHI TCATGA 2 cut(s) 1215, 2073
BspLI GGNNCC 5 cut(s) 41, 2244, 2349, 2510, 2688
BspMAI CTGCAG 2 cut(s) 707, 1308
BspMI ACCTGC 1 cut(s) 51
BspPI GGATC 3 cut(s) 453, 2099, 2600
BspQI GCTCTTC 2 cut(s) 1237, 1959
BspT104I TTCGAA 4 cut(s) 227, 522, 794, 2889
BsrDI GCAATG 2 cut(s) 762, 1041
BsrI ACTGG 4 cut(s) 148, 422, 922, 1833
BssECI CCNNGG 5 cut(s) 471, 1236, 1753, 1774, 2811
BssMI GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
BssNI GRCGYC 1 cut(s) 2328
BssT1I CCWWGG 3 cut(s) 471, 1236, 2811
Bst2UI CCWGG 4 cut(s) 1754, 1775, 2273, 2610
Bst4CI ACNGT 7 cut(s) 755, 1424, 1435, 1604, 1809, 2193, 2740
Bst6I CTCTTC 4 cut(s) 1110, 1237, 1532, 1959
BstACI GRCGYC 1 cut(s) 2328
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstBAI YACGTR 1 cut(s) 2198
BstBI TTCGAA 4 cut(s) 227, 522, 794, 2889
BstC8I GCNNGC 2 cut(s) 789, 1268
BstDSI CCRYGG 2 cut(s) 1236, 2811
BstFNI CGCG 2 cut(s) 760, 2754
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 6 cut(s) 673, 691, 762, 2467, 2754, 2840
BstKTI GATC 9 cut(s) 448, 744, 1092, 1672, 1935, 2107, 2281, 2608, 2928
BstMAI GTCTC 4 cut(s) 365, 887, 1667, 2335
BstMBI GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
BstMCI CGRYCG 1 cut(s) 1082
BstMWI GCNNNNNNNGC 9 cut(s) 697, 1019, 1178, 1907, 2156, 2471, 2705, 2828, 2837
BstNI CCWGG 4 cut(s) 1754, 1775, 2273, 2610
BstSCI CCNGG 5 cut(s) 36, 1752, 1773, 2271, 2608
BstSFI CTRYAG 4 cut(s) 375, 703, 1304, 2139
BstUI CGCG 2 cut(s) 760, 2754
BstV1I GCAGC 9 cut(s) 178, 655, 875, 1287, 1290, 1492, 2137, 2157, 2815
BstV2I GAAGAC 3 cut(s) 120, 1851, 2878
BstX2I RGATCY 3 cut(s) 1669, 1932, 2104
BstYI RGATCY 3 cut(s) 1669, 1932, 2104
BstZI CGGCCG 1 cut(s) 1079
Bsu15I ATCGAT 1 cut(s) 501
BsuI GTATCC 3 cut(s) 1933, 2030, 2485
BsuRI GGCC 7 cut(s) 239, 1081, 1248, 1469, 1901, 2084, 2597
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 2 cut(s) 1236, 2811
BtgZI GCGATG 1 cut(s) 1497
BtrI CACGTC 1 cut(s) 2002
BtsIMutI CAGTG 5 cut(s) 929, 1482, 1570, 2259, 2736
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 2 cut(s) 789, 1268
CaiI CAGNNNCTG 2 cut(s) 1175, 1807
CciI TCATGA 2 cut(s) 1215, 2073
CfoI GCGC 6 cut(s) 673, 691, 762, 2467, 2754, 2840
Cfr13I GGNCC 3 cut(s) 40, 2203, 2509
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 3 cut(s) 756, 2317, 2886
Csp6I GTAC 3 cut(s) 597, 1084, 1805
CviQI GTAC 3 cut(s) 597, 1084, 1805
DpnI GATC 9 cut(s) 447, 743, 1091, 1671, 1934, 2106, 2280, 2607, 2927
DpnII GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
DraI TTTAAA 1 cut(s) 1387
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 4 cut(s) 1079, 1246, 1467, 2595
EagI CGGCCG 1 cut(s) 1079
Eam1104I CTCTTC 4 cut(s) 1110, 1237, 1532, 1959
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 4 cut(s) 1110, 1237, 1532, 1959
Ecl136II GAGCTC 1 cut(s) 1891
EclXI CGGCCG 1 cut(s) 1079
Eco130I CCWWGG 3 cut(s) 471, 1236, 2811
Eco24I GRGCYC 3 cut(s) 702, 931, 1893
Eco32I GATATC 2 cut(s) 1162, 1199
Eco47I GGWCC 3 cut(s) 40, 2203, 2509
Eco47III AGCGCT 1 cut(s) 690
Eco52I CGGCCG 1 cut(s) 1079
Eco53kI GAGCTC 1 cut(s) 1891
Eco57I CTGAAG 6 cut(s) 314, 665, 1863, 2274, 2775, 2844
Eco72I CACGTG 1 cut(s) 2198
Eco88I CYCGRG 1 cut(s) 1517
EcoICRI GAGCTC 1 cut(s) 1891
EcoO109I RGGNCCY 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 2185
EcoRII CCWGG 4 cut(s) 1752, 1773, 2271, 2608
EcoRV GATATC 2 cut(s) 1162, 1199
EcoT14I CCWWGG 3 cut(s) 471, 1236, 2811
EcoT22I ATGCAT 4 cut(s) 910, 2375, 2618, 2638
EcoT38I GRGCYC 3 cut(s) 702, 931, 1893
ErhI CCWWGG 3 cut(s) 471, 1236, 2811
Esp3I CGTCTC 2 cut(s) 1667, 2335
FalI AAGNNNNNCTT 4 cut(s) 252, 284, 2451, 2483
FaqI GGGAC 4 cut(s) 53, 787, 1690, 2522
FauNDI CATATG 2 cut(s) 2449, 2602
FbaI TGATCA 2 cut(s) 741, 1089
FblI GTMKAC 1 cut(s) 1398
FriOI GRGCYC 3 cut(s) 702, 931, 1893
FspBI CTAG 7 cut(s) 710, 1136, 1499, 1656, 2102, 2225, 2835
GlaI GCGC 6 cut(s) 672, 690, 761, 2466, 2753, 2839
GsuI CTGGAG 1 cut(s) 2195
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 7 cut(s) 239, 1081, 1248, 1469, 1901, 2084, 2597
HapII CCGG 6 cut(s) 37, 47, 198, 674, 2525, 2690
HgaI GACGC 3 cut(s) 756, 2317, 2886
HhaI GCGC 6 cut(s) 673, 691, 762, 2467, 2754, 2840
Hin1I GRCGYC 1 cut(s) 2328
Hin6I GCGC 6 cut(s) 671, 689, 760, 2465, 2752, 2838
HinP1I GCGC 6 cut(s) 671, 689, 760, 2465, 2752, 2838
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 1 cut(s) 1835
HpaII CCGG 6 cut(s) 37, 47, 198, 674, 2525, 2690
HphI GGTGA 5 cut(s) 424, 725, 1318, 1441, 1769
Hpy166II GTNNAC 6 cut(s) 332, 639, 1008, 1130, 1399, 2203
Hpy8I GTNNAC 6 cut(s) 332, 639, 1008, 1130, 1399, 2203
Hpy99I CGWCG 3 cut(s) 110, 122, 1160
HpyCH4III ACNGT 7 cut(s) 755, 1424, 1435, 1604, 1809, 2193, 2740
HpyCH4IV ACGT 3 cut(s) 2001, 2197, 2918
HpyF10VI GCNNNNNNNGC 9 cut(s) 697, 1019, 1178, 1907, 2156, 2471, 2705, 2828, 2837
HpySE526I ACGT 3 cut(s) 2001, 2197, 2918
Hsp92I GRCGYC 1 cut(s) 2328
HspAI GCGC 6 cut(s) 671, 689, 760, 2465, 2752, 2838
Ksp22I TGATCA 2 cut(s) 741, 1089
Kzo9I GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
LguI GCTCTTC 2 cut(s) 1237, 1959
LmnI GCTCC 4 cut(s) 181, 826, 1876, 1888
Lsp1109I GCAGC 9 cut(s) 178, 655, 875, 1287, 1290, 1492, 2137, 2157, 2815
MaeI CTAG 7 cut(s) 710, 1136, 1499, 1656, 2102, 2225, 2835
MaeII ACGT 3 cut(s) 2001, 2197, 2918
MalI GATC 9 cut(s) 447, 743, 1091, 1671, 1934, 2106, 2280, 2607, 2927
MboI GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
MfeI CAATTG 1 cut(s) 734
MflI RGATCY 3 cut(s) 1669, 1932, 2104
MhlI GDGCHC 4 cut(s) 702, 931, 1232, 1893
MlsI TGGCCA 2 cut(s) 1248, 2597
MluNI TGGCCA 2 cut(s) 1248, 2597
MlyI GAGTC 7 cut(s) 131, 1481, 1769, 2138, 2393, 2585, 2771
MmeI TCCRAC 6 cut(s) 96, 150, 555, 1035, 1557, 2070
Mox20I TGGCCA 2 cut(s) 1248, 2597
Mph1103I ATGCAT 4 cut(s) 910, 2375, 2618, 2638
MroXI GAANNNNTTC 3 cut(s) 207, 1119, 2646
MscI TGGCCA 2 cut(s) 1248, 2597
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 2 cut(s) 1248, 2597
MspA1I CMGCKG 2 cut(s) 1281, 2096
MspI CCGG 6 cut(s) 37, 47, 198, 674, 2525, 2690
MspR9I CCNGG 5 cut(s) 38, 1754, 1775, 2273, 2610
MunI CAATTG 1 cut(s) 734
Mva1269I GAATGC 1 cut(s) 2362
MvaI CCWGG 4 cut(s) 1754, 1775, 2273, 2610
MvnI CGCG 2 cut(s) 760, 2754
MwoI GCNNNNNNNGC 9 cut(s) 697, 1019, 1178, 1907, 2156, 2471, 2705, 2828, 2837
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 2 cut(s) 1236, 2811
NdeI CATATG 2 cut(s) 2449, 2602
NdeII GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
NlaIV GGNNCC 5 cut(s) 41, 2244, 2349, 2510, 2688
NmeAIII GCCGAG 1 cut(s) 1495
NmuCI GTSAC 8 cut(s) 307, 713, 1324, 1429, 1565, 1718, 2193, 2308
NsiI ATGCAT 4 cut(s) 910, 2375, 2618, 2638
NspV TTCGAA 4 cut(s) 227, 522, 794, 2889
PaeR7I CTCGAG 1 cut(s) 1517
PagI TCATGA 2 cut(s) 1215, 2073
PciSI GCTCTTC 2 cut(s) 1237, 1959
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 123, 764
PctI GAATGC 1 cut(s) 2362
PdmI GAANNNNTTC 3 cut(s) 207, 1119, 2646
PflFI GACNNNGTC 1 cut(s) 1850
PfoI TCCNGGA 3 cut(s) 36, 2271, 2608
PleI GAGTC 7 cut(s) 130, 1480, 1768, 2138, 2393, 2584, 2770
PmaCI CACGTG 1 cut(s) 2198
PmlI CACGTG 1 cut(s) 2198
PpsI GAGTC 7 cut(s) 130, 1480, 1768, 2138, 2393, 2584, 2770
Ppu21I YACGTR 1 cut(s) 2198
PpuMI RGGWCCY 1 cut(s) 40
PshBI ATTAAT 2 cut(s) 1358, 2703
PsiI TTATAA 1 cut(s) 2552
Psp124BI GAGCTC 1 cut(s) 1893
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 4 cut(s) 1752, 1773, 2271, 2608
PspCI CACGTG 1 cut(s) 2198
PspGI CCWGG 4 cut(s) 1752, 1773, 2271, 2608
PspN4I GGNNCC 5 cut(s) 41, 2244, 2349, 2510, 2688
PspPI GGNCC 3 cut(s) 40, 2203, 2509
PspPPI RGGWCCY 1 cut(s) 40
PspXI VCTCGAGB 1 cut(s) 1517
PstI CTGCAG 2 cut(s) 707, 1308
PstNI CAGNNNCTG 2 cut(s) 1175, 1807
PsuI RGATCY 3 cut(s) 1669, 1932, 2104
PsyI GACNNNGTC 1 cut(s) 1850
PvuII CAGCTG 1 cut(s) 2096
RsaI GTAC 3 cut(s) 598, 1085, 1806
RsaNI GTAC 3 cut(s) 597, 1084, 1805
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 1893
SapI GCTCTTC 2 cut(s) 1237, 1959
Sau3AI GATC 9 cut(s) 445, 741, 1089, 1669, 1932, 2104, 2278, 2605, 2925
Sau96I GGNCC 3 cut(s) 40, 2203, 2509
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 7 cut(s) 131, 1481, 1769, 2138, 2393, 2585, 2771
ScrFI CCNGG 5 cut(s) 38, 1754, 1775, 2273, 2610
SduI GDGCHC 4 cut(s) 702, 931, 1232, 1893
SfcI CTRYAG 4 cut(s) 375, 703, 1304, 2139
Sfr274I CTCGAG 1 cut(s) 1517
SfuI TTCGAA 4 cut(s) 227, 522, 794, 2889
SinI GGWCC 3 cut(s) 40, 2203, 2509
SlaI CTCGAG 1 cut(s) 1517
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 2 cut(s) 858, 1517
SmoI CTYRAG 2 cut(s) 858, 1517
SsiI CCGC 5 cut(s) 78, 1078, 1281, 1368, 2540
SspI AATATT 2 cut(s) 252, 1262
SspMI CTAG 7 cut(s) 710, 1136, 1499, 1656, 2102, 2225, 2835
SstI GAGCTC 1 cut(s) 1893
StyD4I CCNGG 5 cut(s) 36, 1752, 1773, 2271, 2608
StyI CCWWGG 3 cut(s) 471, 1236, 2811
TaaI ACNGT 7 cut(s) 755, 1424, 1435, 1604, 1809, 2193, 2740
TaiI ACGT 3 cut(s) 2004, 2200, 2921
TatI WGTACW 1 cut(s) 596
TauI GCSGC 2 cut(s) 1081, 1284
TscAI CASTG 5 cut(s) 929, 1482, 1570, 2266, 2743
TseFI GTSAC 8 cut(s) 307, 713, 1324, 1429, 1565, 1718, 2193, 2308
TseI GCWGC 9 cut(s) 191, 668, 863, 1300, 1303, 1480, 2150, 2170, 2828
Tsp45I GTSAC 8 cut(s) 307, 713, 1324, 1429, 1565, 1718, 2193, 2308
TspDTI ATGAA 7 cut(s) 519, 1232, 1236, 1391, 1601, 2090, 2958
TspGWI ACGGA 2 cut(s) 85, 123
TspRI CASTG 5 cut(s) 929, 1482, 1570, 2266, 2743
Tth111I GACNNNGTC 1 cut(s) 1850
VpaK11BI GGWCC 3 cut(s) 40, 2203, 2509
VspI ATTAAT 2 cut(s) 1358, 2703
XapI RAATTY 7 cut(s) 206, 401, 410, 538, 603, 781, 2185
XcmI CCANNNNNNNNNTGG 1 cut(s) 2341
XhoI CTCGAG 1 cut(s) 1517
XmiI GTMKAC 1 cut(s) 1398
XmnI GAANNNNTTC 3 cut(s) 207, 1119, 2646
XspI CTAG 7 cut(s) 710, 1136, 1499, 1656, 2102, 2225, 2835
ZrmI AGTACT 1 cut(s) 598
Zsp2I ATGCAT 4 cut(s) 910, 2375, 2618, 2638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.