Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0014667

Physical Location & Seq

Genomic Coordinates Forward (+)

21913237 .. 21916309

Gene Structure 3073 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g27700.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGTGCAGCACCAAACCCTTCACATTCTCCTCCTCCCTCATCTTCCTCATCTCCATCACACTCAACACAGTTGCAGCTGCTTCATCATCATGCCCCATAGATCTGAGCTACGTCACCACCTTCCCATGGGACACCTCCCTCTGCCTCGCCCCAAACGGCACACACTGCTGCCAAACCCTTCTCAGCCTCTTCGGCATCGGCCTCTCCCAACACCTCAAGCAAACCTCCACCTTCCAGCTCCCCAACTCCACCGTCTCCGCCGCCTGCCTCTCCGACTTCCAATCCAACCTCTCCTCCCTCTCCCTCAACTCCACCCTCGTCCCCCTCTGCTTCCAAAACGCCAACCAGTTCGTCGCCAACTCCGCCAGCTGCGCCGGCATCCTCACCGTCGACGACTGGACCGACAAAGTGGGACCCATCAGCGCTATAGACACGTCATGTAAAGGAGATCTCCAGGGACTCACCAGGTGTAGCTCTTGCTTAGATGCAGGCATGGATGTGAACAACCAGCTCAGCGGGCTGGGTCCCAACAACACCAAGTGCTTCTATTTCGCCGTGCTTTACGCTGCGGGGGTAGTGAATGAGTTGGGCCCGGAGGATCCGAGAACCGCGGCGTGTATTCTGGGACTGCCGCTGTCCAGCTCAGCTAACAAGAGTGGGAGTAGTAGTCAGGTGAGCAAGAAGACTCTGCTGAAATGGGTATTTGGGGTCTTGGGGGGTATTGTTGGGATTTTACTTTCTGTTGGGGTTATAGTCATGTACAGGAGGCATGAGATGAAAGGGAGGCAGAATGCTAGGCATGAAGAGTATGTGAGTAGCTTTAGGCTTAGTTTGTTGCCTAATTCTGGTGCAAAGTGGTTTGGGATATCAGAGCTTGAGAAGGCAACTGATGGGTTTGCTCAGAAGAACATGCTAGGCCAAGGAGGGTATGGGGTTGTGTACAAGGGCACATTAGCTGATGGGACTCTTGTTGCTGTGAAGCAGATTCTTGATATGGAGGCTTTGGATTCGAAAGGGGATGAGGAGTTTTCAAATGAGGTGGAGATCATAGGGAAGATTCGACATAGGAATTTGCTGTCGCTTAGAGGGTGTTGTGTTACTAGTGATGATTTTAAAGGGAAAAGGAGGTTCTTGGTTTATGATTTTATGACGAATGGGAATTTGAGTGACCATTTGGGTAGCAGTAAGAGGAGATTGAGTTGGCCTCAGAGAAAGAGTATCATACTTGATGTTGCAAAAGGGCTTGCTTACTTGCACAATGGGATCAAGCCTGCTATTTATCACAGGGATATAAAGGGGACTAACATACTCTTGGACACGGAAATGAAGGCCAAAGTTGCAGACTTCGGTTTGGCCAAGCAAAGTTTAGCAGGGCAGACTCACTTGACAACAAGAGTGGCAGGCACTTACGGTTATTTGGCACCAGAGTATGCTCTCTATGGACAGCTTACGGAGAAGAGCGACGTTTACAGCTTCGGCATTGTGGTTCTTGAGATCATGAGTGGGAGGAAAGTGCTGGACACATCGAATTCCAGCACGAATTTGAATTCAGTTGTTTTGATCACGGATTGGGCGTGGATGCATACGAAAGCGGGGAGTGTGGAGGAGGTTTTTGATGAGACATTGAGGGAGGAAGGGCCAAAGAGTGTCATGGAGAGGTTTGTGAATGTTGGGATTCTGTGTGCTCATGTTATGGTGGCTTTTAGGCCTACTATTTCAGAAGCACTGAGGATGTTGGAGGGTGATATTGATATTCCTAAGTTACCAGATAGGCCAATGCCACTTGGCACTGAGTCTTATAGATCTTCTTTTGGGTATTCTAGCATTCAGGAAATTGATAGATCAAGACCCAGATTTAGTTCTAGCATTGTAACTCCCAAGTCCTCAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

631

Amino Acids

68.44

Weight (kDa)

7.82

Isoelectric Point (pI)

37.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	27 - 182	3.7e-40	SPARK
Pkinase	PF00069	300 - 575	1.8e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	301 - 577	3.3e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014667)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g27700
malus_domestica	MD00G1063500.v1.1 MD15G1343400.v1.1
prunus_persica	Prupe.1G490800_v2.0.a1
pyrus_communis	pycom15g30600 pycom15g30610
rosa_chinensis	RchiOBHm_Chr6g0296571
rosa_laevigata	RLG00000011681
rosa_multiflora	Rmu_sc0001741.1_g000023 Rmu_sc0041908.1_g000001
rosa_roxburghii	Rroxscaffold_7G00171430 Rroxscaffold_7G00178690
rosa_rugosa	Rorug06G0263200
rosa_samantha	Rh6AG374900 Rh6CG388400 Rh6DG375800
rosa_wichuraiana	Rw6G032680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1420
AccI	GTMKAC	1 cut(s)	390
AccII	CGCG	1 cut(s)	611
AciI	CCGC	9 cut(s)	258, 261, 363, 516, 569, 609, 611, 632, 1592
AclWI	GGATC	3 cut(s)	593, 606, 1271
AcoI	YGGCCR	1 cut(s)	1353
AcsI	RAATTY	5 cut(s)	1069, 1159, 1528, 1540, 1546
AdeI	CACNNNGTG	2 cut(s)	468, 540
AfaI	GTAC	2 cut(s)	761, 941
AfeI	AGCGCT	1 cut(s)	424
AfiI	CCNNNNNNNGG	2 cut(s)	126, 845
AflIII	ACRYGT	1 cut(s)	432
AgsI	TTSAA	2 cut(s)	1032, 1546
AhlI	ACTAGT	1 cut(s)	1100
AjiI	CACGTC	1 cut(s)	435
AjnI	CCWGG	2 cut(s)	453, 464
Alw21I	GWGCWC	1 cut(s)	1687
Alw26I	GTCTC	2 cut(s)	259, 1613
AlwI	GGATC	3 cut(s)	593, 606, 1271
Aor51HI	AGCGCT	1 cut(s)	424
AoxI	GGCC	9 cut(s)	199, 589, 916, 1202, 1329, 1353, 1637, 1706, 1772
ApaI	GGGCCC	1 cut(s)	593
ApeKI	GCWGC	6 cut(s)	6, 74, 77, 168, 369, 566
ApoI	RAATTY	5 cut(s)	1069, 1159, 1528, 1540, 1546
AspLEI	GCGC	2 cut(s)	374, 425
AspS9I	GGNCC	6 cut(s)	399, 413, 524, 589, 590, 1637
AsuC2I	CCSGG	1 cut(s)	593
AsuHPI	GGTGA	5 cut(s)	106, 376, 454, 685, 1754
AsuII	TTCGAA	1 cut(s)	1010
AvaII	GGWCC	3 cut(s)	399, 413, 524
BaeGI	GKGCMC	2 cut(s)	593, 950
BalI	TGGCCA	1 cut(s)	1355
BamHI	GGATCC	1 cut(s)	598
BanI	GGYRCC	1 cut(s)	1420
BanII	GRGCYC	1 cut(s)	593
BbsI	GAAGAC	1 cut(s)	689
Bbv12I	GWGCWC	1 cut(s)	1687
BbvI	GCAGC	6 cut(s)	18, 64, 86, 155, 356, 553
BccI	CCATC	4 cut(s)	62, 425, 884, 953
BceAI	ACGGC	2 cut(s)	172, 539
BciT130I	CCWGG	2 cut(s)	455, 466
BclI	TGATCA	1 cut(s)	1560
BcnI	CCSGG	1 cut(s)	593
BcoDI	GTCTC	2 cut(s)	259, 1613
BcuI	ACTAGT	1 cut(s)	1100
BfaI	CTAG	5 cut(s)	795, 914, 1101, 1821, 1863
BfmI	CTRYAG	1 cut(s)	426
BfoI	RGCGCY	1 cut(s)	426
BglI	GCCNNNNNGGC	1 cut(s)	192
BglII	AGATCT	3 cut(s)	100, 448, 1802
BisI	GCNGC	9 cut(s)	7, 75, 78, 169, 261, 370, 567, 612, 632
BlpI	GCTNAGC	2 cut(s)	512, 643
BlsI	GCNGC	9 cut(s)	8, 76, 79, 170, 262, 371, 568, 613, 633
Bme1390I	CCNGG	3 cut(s)	455, 466, 593
Bme18I	GGWCC	3 cut(s)	399, 413, 524
BmgBI	CACGTC	1 cut(s)	435
BmgT120I	GGNCC	6 cut(s)	399, 413, 524, 589, 590, 1637
BmiI	GGNNCC	7 cut(s)	414, 415, 525, 526, 591, 600, 1422
BmrFI	CCNGG	3 cut(s)	455, 466, 593
BmsI	GCATC	4 cut(s)	204, 387, 475, 1569
BpiI	GAAGAC	1 cut(s)	689
BplI	GAGNNNNNCTC	2 cut(s)	1189, 1221
BpmI	CTGGAG	1 cut(s)	437
Bpu1102I	GCTNAGC	2 cut(s)	512, 643
Bpu14I	TTCGAA	1 cut(s)	1010
BpuEI	CTTGAG	3 cut(s)	200, 896, 1511
BpuMI	CCSGG	1 cut(s)	593
BsaJI	CCNNGG	4 cut(s)	125, 454, 609, 919
BsaXI	ACNNNNNCTCC	6 cut(s)	278, 308, 652, 682, 1183, 1213
Bsc4I	CCNNNNNNNGG	2 cut(s)	126, 845
Bse118I	RCCGGY	1 cut(s)	374
Bse1I	ACTGG	2 cut(s)	346, 401
BseBI	CCWGG	2 cut(s)	455, 466
BseDI	CCNNGG	4 cut(s)	125, 454, 609, 919
BseGI	GGATG	5 cut(s)	378, 502, 1024, 1584, 1737
BseLI	CCNNNNNNNGG	2 cut(s)	126, 845
BseMII	CTCAG	8 cut(s)	95, 196, 526, 657, 914, 1220, 1718, 1782
BseNI	ACTGG	2 cut(s)	346, 401
BseRI	GAGGAG	6 cut(s)	19, 22, 283, 1037, 1204, 1619
BseSI	GKGCMC	2 cut(s)	593, 950
BseXI	GCAGC	6 cut(s)	18, 64, 86, 155, 356, 553
BseYI	CCCAGC	1 cut(s)	520
BsgI	GTGCAG	1 cut(s)	25
Bsh1236I	CGCG	1 cut(s)	611
BshFI	GGCC	9 cut(s)	201, 591, 918, 1204, 1331, 1355, 1639, 1708, 1774
BshNI	GGYRCC	1 cut(s)	1420
BsiHKAI	GWGCWC	1 cut(s)	1687
BsiSI	CCGG	2 cut(s)	375, 593
BslFI	GGGAC	8 cut(s)	143, 305, 426, 471, 510, 639, 976, 1312
BslI	CCNNNNNNNGG	2 cut(s)	126, 845
BsmAI	GTCTC	2 cut(s)	259, 1613
BsmBI	CGTCTC	1 cut(s)	259
BsmFI	GGGAC	8 cut(s)	143, 305, 426, 471, 510, 639, 976, 1312
BsmI	GAATGC	2 cut(s)	796, 1824
BsnI	GGCC	9 cut(s)	201, 591, 918, 1204, 1331, 1355, 1639, 1708, 1774
Bsp119I	TTCGAA	1 cut(s)	1010
Bsp120I	GGGCCC	1 cut(s)	589
Bsp1286I	GDGCHC	3 cut(s)	593, 950, 1687
Bsp1407I	TGTACA	2 cut(s)	759, 939
Bsp143I	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
Bsp1720I	GCTNAGC	2 cut(s)	512, 643
Bsp19I	CCATGG	1 cut(s)	125
BspACI	CCGC	9 cut(s)	258, 261, 363, 516, 569, 609, 611, 632, 1592
BspANI	GGCC	9 cut(s)	201, 591, 918, 1204, 1331, 1355, 1639, 1708, 1774
BspCNI	CTCAG	8 cut(s)	96, 195, 525, 656, 913, 1219, 1719, 1783
BspFNI	CGCG	1 cut(s)	611
BspHI	TCATGA	1 cut(s)	1497
BspLI	GGNNCC	7 cut(s)	414, 415, 525, 526, 591, 600, 1422
BspPI	GGATC	3 cut(s)	593, 606, 1271
BspQI	GCTCTTC	1 cut(s)	1451
BspT104I	TTCGAA	1 cut(s)	1010
BspT107I	GGYRCC	1 cut(s)	1420
BsrFI	RCCGGY	1 cut(s)	374
BsrGI	TGTACA	2 cut(s)	759, 939
BsrI	ACTGG	2 cut(s)	346, 401
BssAI	RCCGGY	1 cut(s)	374
BssECI	CCNNGG	4 cut(s)	125, 454, 609, 919
BssMI	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
BssT1I	CCWWGG	2 cut(s)	125, 919
Bst2UI	CCWGG	2 cut(s)	455, 466
Bst4CI	ACNGT	4 cut(s)	70, 253, 388, 1412
Bst6I	CTCTTC	3 cut(s)	194, 798, 1451
BstAPI	GCANNNNNTGC	1 cut(s)	165
BstAUI	TGTACA	2 cut(s)	759, 939
BstBI	TTCGAA	1 cut(s)	1010
BstC8I	GCNNGC	8 cut(s)	265, 367, 376, 490, 518, 1245, 1272, 1402
BstDSI	CCRYGG	2 cut(s)	125, 609
BstF5I	GGATG	5 cut(s)	378, 502, 1024, 1584, 1737
BstFNI	CGCG	1 cut(s)	611
BstH2I	RGCGCY	1 cut(s)	426
BstHHI	GCGC	2 cut(s)	374, 425
BstKTI	GATC	9 cut(s)	103, 451, 601, 1047, 1266, 1497, 1563, 1805, 1844
BstMAI	GTCTC	2 cut(s)	259, 1613
BstMBI	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
BstMWI	GCNNNNNNNGC	7 cut(s)	165, 192, 362, 371, 375, 517, 1337
BstNI	CCWGG	2 cut(s)	455, 466
BstNSI	RCATGY	1 cut(s)	913
BstSCI	CCNGG	3 cut(s)	453, 464, 591
BstSFI	CTRYAG	1 cut(s)	426
BstSLI	GKGCMC	2 cut(s)	593, 950
BstUI	CGCG	1 cut(s)	611
BstV1I	GCAGC	6 cut(s)	18, 64, 86, 155, 356, 553
BstV2I	GAAGAC	1 cut(s)	689
BstX2I	RGATCY	4 cut(s)	100, 448, 598, 1802
BstYI	RGATCY	4 cut(s)	100, 448, 598, 1802
BsuRI	GGCC	9 cut(s)	201, 591, 918, 1204, 1331, 1355, 1639, 1708, 1774
BtgI	CCRYGG	2 cut(s)	125, 609
BtrI	CACGTC	1 cut(s)	435
BtsCI	GGATG	5 cut(s)	378, 502, 1024, 1584, 1737
BtsI	GCAGTG	1 cut(s)	163
BtsIMutI	CAGTG	3 cut(s)	163, 1724, 1788
Cac8I	GCNNGC	8 cut(s)	265, 367, 376, 490, 518, 1245, 1272, 1402
CciI	TCATGA	1 cut(s)	1497
CfoI	GCGC	2 cut(s)	374, 425
Cfr10I	RCCGGY	1 cut(s)	374
Cfr13I	GGNCC	6 cut(s)	399, 413, 524, 589, 590, 1637
Cfr42I	CCGCGG	1 cut(s)	612
CsiI	ACCWGGT	1 cut(s)	464
Csp6I	GTAC	2 cut(s)	760, 940
CspCI	CAANNNNNGTGG	4 cut(s)	1020, 1055, 1377, 1412
CviQI	GTAC	2 cut(s)	760, 940
DpnI	GATC	9 cut(s)	102, 450, 600, 1046, 1265, 1496, 1562, 1804, 1843
DpnII	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
DraI	TTTAAA	1 cut(s)	1114
DraIII	CACNNNGTG	2 cut(s)	468, 540
EaeI	YGGCCR	1 cut(s)	1353
Eam1104I	CTCTTC	3 cut(s)	194, 798, 1451
EarI	CTCTTC	3 cut(s)	194, 798, 1451
EciI	GGCGGA	2 cut(s)	247, 352
Eco130I	CCWWGG	2 cut(s)	125, 919
Eco147I	AGGCCT	1 cut(s)	1708
Eco24I	GRGCYC	1 cut(s)	593
Eco32I	GATATC	1 cut(s)	867
Eco47I	GGWCC	3 cut(s)	399, 413, 524
Eco47III	AGCGCT	1 cut(s)	424
EcoO109I	RGGNCCY	2 cut(s)	413, 524
EcoRI	GAATTC	2 cut(s)	1528, 1546
EcoRII	CCWGG	2 cut(s)	453, 464
EcoRV	GATATC	1 cut(s)	867
EcoT14I	CCWWGG	2 cut(s)	125, 919
EcoT22I	ATGCAT	1 cut(s)	1584
EcoT38I	GRGCYC	1 cut(s)	593
ErhI	CCWWGG	2 cut(s)	125, 919
Esp3I	CGTCTC	1 cut(s)	259
FaqI	GGGAC	8 cut(s)	143, 305, 426, 471, 510, 639, 976, 1312
FauI	CCCGC	3 cut(s)	509, 562, 1585
FbaI	TGATCA	1 cut(s)	1560
FblI	GTMKAC	1 cut(s)	390
Fnu4HI	GCNGC	9 cut(s)	7, 75, 78, 169, 261, 370, 567, 612, 632
FokI	GGATG	5 cut(s)	365, 509, 1031, 1591, 1744
FriOI	GRGCYC	1 cut(s)	593
Fsp4HI	GCNGC	9 cut(s)	7, 75, 78, 169, 261, 370, 567, 612, 632
FspBI	CTAG	5 cut(s)	795, 914, 1101, 1821, 1863
GlaI	GCGC	2 cut(s)	373, 424
GluI	GCNGC	9 cut(s)	7, 75, 78, 169, 261, 370, 567, 612, 632
GsaI	CCCAGC	1 cut(s)	524
GsuI	CTGGAG	1 cut(s)	437
HaeII	RGCGCY	1 cut(s)	426
HaeIII	GGCC	9 cut(s)	201, 591, 918, 1204, 1331, 1355, 1639, 1708, 1774
HapII	CCGG	2 cut(s)	375, 593
HhaI	GCGC	2 cut(s)	374, 425
Hin6I	GCGC	2 cut(s)	372, 423
HinP1I	GCGC	2 cut(s)	372, 423
HincII	GTYRAC	1 cut(s)	391
HindII	GTYRAC	1 cut(s)	391
HinfI	GANTC	9 cut(s)	459, 685, 964, 985, 1007, 1057, 1378, 1675, 1793
HpaII	CCGG	2 cut(s)	375, 593
HphI	GGTGA	5 cut(s)	106, 376, 454, 685, 1754
Hpy166II	GTNNAC	4 cut(s)	391, 502, 940, 1468
Hpy188I	TCNGA	7 cut(s)	105, 274, 603, 871, 903, 1209, 1720
Hpy188III	TCNNGA	5 cut(s)	989, 1490, 1498, 1829, 1845
Hpy8I	GTNNAC	4 cut(s)	391, 502, 940, 1468
Hpy99I	CGWCG	4 cut(s)	356, 392, 395, 1466
HpyAV	CCTTC	7 cut(s)	28, 130, 188, 241, 874, 1321, 1628
HpyCH4III	ACNGT	4 cut(s)	70, 253, 388, 1412
HpyCH4IV	ACGT	3 cut(s)	111, 434, 1464
HpyCH4V	TGCA	8 cut(s)	6, 74, 488, 851, 1235, 1255, 1340, 1582
HpyF10VI	GCNNNNNNNGC	7 cut(s)	165, 192, 362, 371, 375, 517, 1337
HpySE526I	ACGT	3 cut(s)	111, 434, 1464
HspAI	GCGC	2 cut(s)	372, 423
KflI	GGGWCCC	2 cut(s)	413, 524
KroI	GCCGGC	1 cut(s)	374
KroNI	GCCGGC	1 cut(s)	376
Ksp22I	TGATCA	1 cut(s)	1560
KspI	CCGCGG	1 cut(s)	612
Kzo9I	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
LguI	GCTCTTC	1 cut(s)	1451
LmnI	GCTCC	1 cut(s)	243
Lsp1109I	GCAGC	6 cut(s)	18, 64, 86, 155, 356, 553
LweI	GCATC	4 cut(s)	204, 387, 475, 1569
MabI	ACCWGGT	1 cut(s)	464
MaeI	CTAG	5 cut(s)	795, 914, 1101, 1821, 1863
MaeII	ACGT	3 cut(s)	111, 434, 1464
MaeIII	GTNAC	5 cut(s)	112, 1096, 1166, 1761, 1870
MalI	GATC	9 cut(s)	102, 450, 600, 1046, 1265, 1496, 1562, 1804, 1843
MboI	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
MboII	GAAGA	8 cut(s)	34, 181, 694, 815, 916, 1066, 1468, 1797
MflI	RGATCY	4 cut(s)	100, 448, 598, 1802
MhlI	GDGCHC	3 cut(s)	593, 950, 1687
MlsI	TGGCCA	1 cut(s)	1355
MluCI	AATT	7 cut(s)	841, 1069, 1159, 1528, 1540, 1546, 1833
MluNI	TGGCCA	1 cut(s)	1355
MlyI	GAGTC	5 cut(s)	453, 679, 958, 1372, 1802
MmeI	TCCRAC	3 cut(s)	297, 309, 1716
Mox20I	TGGCCA	1 cut(s)	1355
Mph1103I	ATGCAT	1 cut(s)	1584
MroNI	GCCGGC	1 cut(s)	374
MscI	TGGCCA	1 cut(s)	1355
MseI	TTAA	1 cut(s)	1113
MslI	CAYNNNNRTG	3 cut(s)	88, 497, 1322
Msp20I	TGGCCA	1 cut(s)	1355
MspA1I	CMGCKG	5 cut(s)	77, 369, 516, 611, 634
MspI	CCGG	2 cut(s)	375, 593
MspR9I	CCNGG	3 cut(s)	455, 466, 593
Mva1269I	GAATGC	2 cut(s)	796, 1824
MvaI	CCWGG	2 cut(s)	455, 466
MvnI	CGCG	1 cut(s)	611
MwoI	GCNNNNNNNGC	7 cut(s)	165, 192, 362, 371, 375, 517, 1337
NaeI	GCCGGC	1 cut(s)	376
NciI	CCSGG	1 cut(s)	593
NcoI	CCATGG	1 cut(s)	125
NdeII	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
NgoMIV	GCCGGC	1 cut(s)	374
NlaIV	GGNNCC	7 cut(s)	414, 415, 525, 526, 591, 600, 1422
NmuCI	GTSAC	2 cut(s)	112, 1166
NsiI	ATGCAT	1 cut(s)	1584
NspI	RCATGY	1 cut(s)	913
NspV	TTCGAA	1 cut(s)	1010
PagI	TCATGA	1 cut(s)	1497
PceI	AGGCCT	1 cut(s)	1708
PciSI	GCTCTTC	1 cut(s)	1451
PcsI	WCGNNNNNNNCGW	2 cut(s)	399, 1571
PctI	GAATGC	2 cut(s)	796, 1824
PdiI	GCCGGC	1 cut(s)	376
PfeI	GAWTC	4 cut(s)	985, 1007, 1057, 1675
PkrI	GCNGC	9 cut(s)	8, 76, 79, 170, 262, 371, 568, 613, 633
PleI	GAGTC	5 cut(s)	453, 679, 958, 1372, 1801
PpsI	GAGTC	5 cut(s)	453, 679, 958, 1372, 1801
PpuMI	RGGWCCY	2 cut(s)	413, 524
Psp5II	RGGWCCY	2 cut(s)	413, 524
Psp6I	CCWGG	2 cut(s)	453, 464
PspFI	CCCAGC	1 cut(s)	520
PspGI	CCWGG	2 cut(s)	453, 464
PspN4I	GGNNCC	7 cut(s)	414, 415, 525, 526, 591, 600, 1422
PspOMI	GGGCCC	1 cut(s)	589
PspPI	GGNCC	6 cut(s)	399, 413, 524, 589, 590, 1637
PspPPI	RGGWCCY	2 cut(s)	413, 524
PsuI	RGATCY	4 cut(s)	100, 448, 598, 1802
PvuII	CAGCTG	2 cut(s)	77, 369
RsaI	GTAC	2 cut(s)	761, 941
RsaNI	GTAC	2 cut(s)	760, 940
RseI	CAYNNNNRTG	3 cut(s)	88, 497, 1322
SacII	CCGCGG	1 cut(s)	612
SalI	GTCGAC	1 cut(s)	389
SapI	GCTCTTC	1 cut(s)	1451
SaqAI	TTAA	1 cut(s)	1113
SatI	GCNGC	9 cut(s)	7, 75, 78, 169, 261, 370, 567, 612, 632
Sau3AI	GATC	9 cut(s)	100, 448, 598, 1044, 1263, 1494, 1560, 1802, 1841
Sau96I	GGNCC	6 cut(s)	399, 413, 524, 589, 590, 1637
SchI	GAGTC	5 cut(s)	453, 679, 958, 1372, 1802
ScrFI	CCNGG	3 cut(s)	455, 466, 593
SduI	GDGCHC	3 cut(s)	593, 950, 1687
SexAI	ACCWGGT	1 cut(s)	464
SfaNI	GCATC	4 cut(s)	204, 387, 475, 1569
SfcI	CTRYAG	1 cut(s)	426
Sfr303I	CCGCGG	1 cut(s)	612
SfuI	TTCGAA	1 cut(s)	1010
SgrBI	CCGCGG	1 cut(s)	612
SgrDI	CGTCGACG	1 cut(s)	389
SinI	GGWCC	3 cut(s)	399, 413, 524
SmiMI	CAYNNNNRTG	3 cut(s)	88, 497, 1322
SmlI	CTYRAG	3 cut(s)	215, 875, 1490
SmoI	CTYRAG	3 cut(s)	215, 875, 1490
SpeI	ACTAGT	1 cut(s)	1100
Sse9I	AATT	7 cut(s)	841, 1069, 1159, 1528, 1540, 1546, 1833
SseBI	AGGCCT	1 cut(s)	1708
SsiI	CCGC	9 cut(s)	258, 261, 363, 516, 569, 609, 611, 632, 1592
SspMI	CTAG	5 cut(s)	795, 914, 1101, 1821, 1863
StuI	AGGCCT	1 cut(s)	1708
StyD4I	CCNGG	3 cut(s)	453, 464, 591
StyI	CCWWGG	2 cut(s)	125, 919
TaaI	ACNGT	4 cut(s)	70, 253, 388, 1412
TaiI	ACGT	3 cut(s)	114, 437, 1467
TaqI	TCGA	4 cut(s)	390, 1010, 1060, 1526
TaqII	GACCGA	1 cut(s)	416
TasI	AATT	7 cut(s)	841, 1069, 1159, 1528, 1540, 1546, 1833
TatI	WGTACW	2 cut(s)	759, 939
TauI	GCSGC	3 cut(s)	263, 614, 634
TfiI	GAWTC	4 cut(s)	985, 1007, 1057, 1675
Tru1I	TTAA	1 cut(s)	1113
Tru9I	TTAA	1 cut(s)	1113
TscAI	CASTG	3 cut(s)	170, 1731, 1795
TseFI	GTSAC	2 cut(s)	112, 1166
TseI	GCWGC	6 cut(s)	6, 74, 77, 168, 369, 566
Tsp45I	GTSAC	2 cut(s)	112, 1166
TspDTI	ATGAA	4 cut(s)	72, 791, 816, 1340
TspGWI	ACGGA	3 cut(s)	1334, 1466, 1580
TspRI	CASTG	3 cut(s)	170, 1731, 1795
VpaK11BI	GGWCC	3 cut(s)	399, 413, 524
XapI	RAATTY	5 cut(s)	1069, 1159, 1528, 1540, 1546
XceI	RCATGY	1 cut(s)	913
XcmI	CCANNNNNNNNNTGG	1 cut(s)	926
XmiI	GTMKAC	1 cut(s)	390
XspI	CTAG	5 cut(s)	795, 914, 1101, 1821, 1863
Zsp2I	ATGCAT	1 cut(s)	1584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.