FvH4_2g29441

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0019204
Physical Location & Seq
Reverse (-)
22833703 .. 22835655
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29441.t1

Sequence Viewer

Length: 765 bp
ATGGAAACATTACCAAGGTCAGTGACCTCATTTAGGAGGCAAGGCTCTTCAGGGTTAGTATGGGATGAGGTATTACAAAAAGAGGTGGCCAGGACAGAGTTTAGAGAGCTAAGGCTCTGCCACAGTGCCAAGCCCCTTGGTATGGTGTATAGCAATTCAAATGTAGAACTCACCATTTGCACCCGCAGTAATTCTACCCCAGCCAAAAAGCATTCTGCTCCTATTTCTGATCAACTGGCATATCAACAAAATGGTATTCCACCAGATCACTTTCGTGTAAATGACGAGAAATACAACCGGGTCGGTCTTTGCATTAAAAAATTTGGAGCCCGGGGAAAGCCCGGCTCGGCAAAAGCCCAGACCGGTTTGGCCGGGTTTCGGGCCTATTTTGCCGTCGTCGAGAGGAAGAGAAGAGAGGTACCTGAGACCTTTCTCGTTGAAGGTGACGGCGATGGAGCCCTCTTCTGCCACGCTGAGCTGCCGCCGGAATCGCCGCGTCTTCCGCCTTTTCTGCTGCCACGGCGGCTTCCCCAATTTGTGAGAGAACTCAGAACTCTGCTCGTTGCACGGAATATGTGCGCTGGCATCGCTGAGACTCAAGAGGCGCGCAGGCGGCAGGTGTCGTCGAGAGGCATGGAGGCACGGAGGCTTGGAGGCGCGGAGGGCGAAGTGCGGAGGGCGAAGGGCGGAGGCGTTGAGAAGAGCAGCGTGTTTCGTCTCTCAGCATCTCTGTTTGGTGTAAACTATGAAGTTTATGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

255

Amino Acids

28.36

Weight (kDa)

9.85

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 63 1.9e-09 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019204)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29441
prunus_persica Prupe.1G478300_v2.0.a1
rosa_laevigata RLG00000011522
rosa_roxburghii Rroxscaffold_7G00169830
rosa_samantha Rh6AG390100 Rh6BG398500 Rh6CG404400 Rh6DG391100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 607
Acc36I ACCTGC 1 cut(s) 607
Acc65I GGTACC 1 cut(s) 418
AccB1I GGYRCC 1 cut(s) 418
AccII CGCG 3 cut(s) 496, 607, 659
AciI CCGC 9 cut(s) 184, 482, 494, 503, 523, 613, 659, 673, 687
AcoI YGGCCR 2 cut(s) 87, 369
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 1 cut(s) 420
AfiI CCNNNNNNNGG 3 cut(s) 33, 142, 378
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 2 cut(s) 159, 440
AjnI CCWGG 1 cut(s) 89
AleI CACNNNNGTG 1 cut(s) 273
AluBI AGCT 2 cut(s) 109, 478
AluI AGCT 2 cut(s) 109, 478
Alw26I GTCTC 3 cut(s) 419, 587, 722
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 3 cut(s) 87, 369, 381
ApeKI GCWGC 3 cut(s) 478, 514, 705
ApoI RAATTY 1 cut(s) 320
AsiGI ACCGGT 1 cut(s) 362
Asp718I GGTACC 1 cut(s) 418
AspLEI GCGC 4 cut(s) 581, 607, 609, 659
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 5 cut(s) 299, 331, 332, 342, 373
AsuHPI GGTGA 2 cut(s) 163, 455
AvaI CYCGRG 1 cut(s) 330
BalI TGGCCA 1 cut(s) 89
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 2 cut(s) 331, 460
BbsI GAAGAC 1 cut(s) 491
BbvI GCAGC 3 cut(s) 465, 501, 717
BccI CCATC 1 cut(s) 446
BceAI ACGGC 3 cut(s) 377, 463, 536
BciT130I CCWGG 1 cut(s) 91
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 5 cut(s) 299, 331, 332, 342, 373
BcoDI GTCTC 3 cut(s) 419, 587, 722
BfuAI ACCTGC 1 cut(s) 607
BglI GCCNNNNNGGC 1 cut(s) 523
BisI GCNGC 7 cut(s) 479, 482, 494, 515, 524, 614, 706
BlpI GCTNAGC 1 cut(s) 474
BlsI GCNGC 7 cut(s) 480, 483, 495, 516, 525, 615, 707
Bme1390I CCNGG 6 cut(s) 91, 299, 331, 332, 342, 373
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 3 cut(s) 328, 420, 457
BmrFI CCNGG 6 cut(s) 91, 299, 331, 332, 342, 373
BmsI GCATC 2 cut(s) 594, 734
BpiI GAAGAC 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 5 cut(s) 299, 331, 332, 342, 373
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 5 cut(s) 14, 136, 330, 331, 518
BsaWI WCCGGW 1 cut(s) 362
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 142, 378
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 5 cut(s) 14, 136, 330, 331, 518
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 3 cut(s) 33, 142, 378
BseMII CTCAG 5 cut(s) 414, 465, 562, 582, 735
BseNI ACTGG 1 cut(s) 240
BsePI GCGCGC 1 cut(s) 605
BseXI GCAGC 3 cut(s) 465, 501, 717
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 3 cut(s) 496, 607, 659
BshFI GGCC 3 cut(s) 89, 371, 383
BshNI GGYRCC 1 cut(s) 418
BshTI ACCGGT 1 cut(s) 362
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 6 cut(s) 298, 331, 342, 363, 372, 485
BslI CCNNNNNNNGG 3 cut(s) 33, 142, 378
BsmAI GTCTC 3 cut(s) 419, 587, 722
BsmBI CGTCTC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 3 cut(s) 89, 371, 383
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 331, 460
Bsp143I GATC 2 cut(s) 229, 265
Bsp1720I GCTNAGC 1 cut(s) 474
BspACI CCGC 9 cut(s) 184, 482, 494, 503, 523, 613, 659, 673, 687
BspANI GGCC 3 cut(s) 89, 371, 383
BspCNI CTCAG 5 cut(s) 415, 466, 561, 583, 734
BspFNI CGCG 3 cut(s) 496, 607, 659
BspLI GGNNCC 3 cut(s) 328, 420, 457
BspMI ACCTGC 1 cut(s) 607
BspQI GCTCTTC 2 cut(s) 52, 695
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 5 cut(s) 14, 136, 330, 331, 518
BssHII GCGCGC 1 cut(s) 605
BssMI GATC 2 cut(s) 229, 265
BssT1I CCWWGG 2 cut(s) 14, 136
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 5 cut(s) 52, 401, 406, 467, 695
BstC8I GCNNGC 3 cut(s) 583, 607, 611
BstDEI CTNAG 6 cut(s) 110, 423, 474, 548, 591, 721
BstDSI CCRYGG 1 cut(s) 518
BstENI CCTNNNNNAGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 3 cut(s) 496, 607, 659
BstHHI GCGC 4 cut(s) 581, 607, 609, 659
BstKTI GATC 2 cut(s) 232, 268
BstMAI GTCTC 3 cut(s) 419, 587, 722
BstMBI GATC 2 cut(s) 229, 265
BstMWI GCNNNNNNNGC 9 cut(s) 389, 490, 502, 511, 520, 523, 587, 613, 663
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 6 cut(s) 89, 297, 329, 330, 340, 371
BstUI CGCG 3 cut(s) 496, 607, 659
BstV1I GCAGC 3 cut(s) 465, 501, 717
BstV2I GAAGAC 1 cut(s) 491
BsuRI GGCC 3 cut(s) 89, 371, 383
BtgI CCRYGG 1 cut(s) 518
BtgZI GCGATG 2 cut(s) 465, 571
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 27, 130
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 3 cut(s) 583, 607, 611
CfoI GCGC 4 cut(s) 581, 607, 609, 659
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 381
Cfr9I CCCGGG 1 cut(s) 330
CseI GACGC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 419
CspAI ACCGGT 1 cut(s) 362
CviAII CATG 1 cut(s) 634
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 6 cut(s) 110, 423, 474, 548, 591, 721
DpnI GATC 2 cut(s) 231, 267
DpnII GATC 2 cut(s) 229, 265
EaeI YGGCCR 2 cut(s) 87, 369
Eam1104I CTCTTC 5 cut(s) 52, 401, 406, 467, 695
EarI CTCTTC 5 cut(s) 52, 401, 406, 467, 695
EciI GGCGGA 2 cut(s) 492, 702
Eco130I CCWWGG 2 cut(s) 14, 136
Eco24I GRGCYC 2 cut(s) 331, 460
Eco31I GGTCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 2 cut(s) 14, 136
EcoT38I GRGCYC 2 cut(s) 331, 460
ErhI CCWWGG 2 cut(s) 14, 136
Esp3I CGTCTC 1 cut(s) 722
FaeI CATG 1 cut(s) 637
FaiI YATR 8 cut(s) 61, 143, 150, 241, 575, 635, 747, 756
FatI CATG 1 cut(s) 633
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 7 cut(s) 479, 482, 494, 515, 524, 614, 706
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 2 cut(s) 331, 460
Fsp4HI GCNGC 7 cut(s) 479, 482, 494, 515, 524, 614, 706
GlaI GCGC 4 cut(s) 580, 606, 608, 658
GluI GCNGC 7 cut(s) 479, 482, 494, 515, 524, 614, 706
GsaI CCCAGC 1 cut(s) 203
HaeIII GGCC 3 cut(s) 89, 371, 383
HapII CCGG 6 cut(s) 298, 331, 342, 363, 372, 485
HgaI GACGC 1 cut(s) 485
HhaI GCGC 4 cut(s) 581, 607, 609, 659
Hin1II CATG 1 cut(s) 637
Hin6I GCGC 4 cut(s) 579, 605, 607, 657
HinP1I GCGC 4 cut(s) 579, 605, 607, 657
HinfI GANTC 2 cut(s) 488, 595
HpaII CCGG 6 cut(s) 298, 331, 342, 363, 372, 485
HphI GGTGA 2 cut(s) 163, 455
Hpy166II GTNNAC 1 cut(s) 742
Hpy188I TCNGA 2 cut(s) 229, 551
Hpy188III TCNNGA 3 cut(s) 400, 599, 627
Hpy8I GTNNAC 1 cut(s) 742
Hpy99I CGWCG 3 cut(s) 398, 401, 628
HpyAV CCTTC 2 cut(s) 434, 676
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 3 cut(s) 180, 312, 566
HpyF10VI GCNNNNNNNGC 9 cut(s) 389, 490, 502, 511, 520, 523, 587, 613, 663
HpyF3I CTNAG 6 cut(s) 110, 423, 474, 548, 591, 721
Hsp92II CATG 1 cut(s) 637
HspAI GCGC 4 cut(s) 579, 605, 607, 657
KpnI GGTACC 1 cut(s) 422
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 229, 265
LguI GCTCTTC 2 cut(s) 52, 695
LmnI GCTCC 3 cut(s) 223, 326, 455
Lsp1109I GCAGC 3 cut(s) 465, 501, 717
LweI GCATC 2 cut(s) 594, 734
MaeIII GTNAC 2 cut(s) 22, 443
MalI GATC 2 cut(s) 231, 267
MboI GATC 2 cut(s) 229, 265
MboII GAAGA 6 cut(s) 39, 418, 423, 454, 491, 712
MhlI GDGCHC 2 cut(s) 331, 460
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 4 cut(s) 154, 190, 320, 533
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 1 cut(s) 589
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 6 cut(s) 298, 331, 342, 363, 372, 485
MspR9I CCNGG 6 cut(s) 91, 299, 331, 332, 342, 373
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 3 cut(s) 496, 607, 659
MwoI GCNNNNNNNGC 9 cut(s) 389, 490, 502, 511, 520, 523, 587, 613, 663
NciI CCSGG 5 cut(s) 299, 331, 332, 342, 373
NdeII GATC 2 cut(s) 229, 265
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 3 cut(s) 328, 420, 457
NmeAIII GCCGAG 1 cut(s) 326
NmuCI GTSAC 2 cut(s) 22, 443
OliI CACNNNNGTG 1 cut(s) 273
PaqCI CACCTGC 1 cut(s) 607
PauI GCGCGC 1 cut(s) 605
PciSI GCTCTTC 2 cut(s) 52, 695
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 488
PinAI ACCGGT 1 cut(s) 362
PkrI GCNGC 7 cut(s) 480, 483, 495, 516, 525, 615, 707
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 3 cut(s) 328, 420, 457
PspPI GGNCC 1 cut(s) 381
PteI GCGCGC 1 cut(s) 605
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 273
SapI GCTCTTC 2 cut(s) 52, 695
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 7 cut(s) 479, 482, 494, 515, 524, 614, 706
Sau3AI GATC 2 cut(s) 229, 265
Sau96I GGNCC 1 cut(s) 381
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 6 cut(s) 91, 299, 331, 332, 342, 373
SduI GDGCHC 2 cut(s) 331, 460
SfaNI GCATC 2 cut(s) 594, 734
SmaI CCCGGG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 4 cut(s) 154, 190, 320, 533
SsiI CCGC 9 cut(s) 184, 482, 494, 503, 523, 613, 659, 673, 687
StyD4I CCNGG 6 cut(s) 89, 297, 329, 330, 340, 371
StyI CCWWGG 2 cut(s) 14, 136
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 2 cut(s) 399, 626
TaqII GACCGA 1 cut(s) 293
TasI AATT 4 cut(s) 154, 190, 320, 533
TauI GCSGC 4 cut(s) 484, 496, 526, 616
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 2 cut(s) 27, 130
TseFI GTSAC 2 cut(s) 22, 443
TseI GCWGC 3 cut(s) 478, 514, 705
Tsp45I GTSAC 2 cut(s) 22, 443
TspDTI ATGAA 1 cut(s) 762
TspGWI ACGGA 2 cut(s) 583, 658
TspMI CCCGGG 1 cut(s) 330
TspRI CASTG 2 cut(s) 27, 130
XagI CCTNNNNNAGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 320
XmaI CCCGGG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.