FvH4_2g38450

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017053
Physical Location & Seq
Forward (+)
27811431 .. 27814655
3225 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38450.t1

Sequence Viewer

Length: 1806 bp
ATGTGGGGAGACTCCCAAGCCGTCATCGGATTTCCCAAGATCACCAAGCCATATATATACCTCTACATTCAGCACCCAACTTCATTTCAGAAGCATAAGTATATCCTGTTACATTGCTCTCTTATCTCTGGTAGTCTGAGATATCTGAAAATCGACATGTCTCCGACTGCGACAGCCGAGAATGGAAATGTAAGAAGTAGTCATGAGGCAGCCGGCAGCATCTTTGACATCGGAAACTCCGATTTTCTCAAGATGTTAGAGAGACCTAGAACAATAAATGTGGAGAGGAAGAGGTCCTTTGATGAAAGGTCATTCAGTGATTTGTCAAACATTGAACACCTCGAGTATATGTCCTCGTCTGTTCTGAACACTATTACGCCTAAGTCATGCTATGACTCGCATCTCATGGTTTCCGATGCTTGGGAGACCTTGCGGCGCTCTGTCGTTCACTTTCGCGGCCAGCCGGTTGGGACTCTTGCTGCCGTCGACAGTTCTGTTGAACAACTTAACTATGATCAGGTATTTGTGAGAGACTTTGTCCCGAGCGCACTGGCCTTTCTGATGAACGGCGAAGAAGAAATAGTGAAGAACTTTCTCTTGAAAACGCTTCGCCTCCAATCATGGGAGAAAATAGTCGACGAGTTTAAGCTAGGAGCAGGGGTAATGCCAGCCAGCTTCAAAGTAGAGCACGACCAAGTCAGAAACAATGACACCATCACTGCAGATTTCGGCGAGAGCGCAATAGGAAGAGTTGCTCCTGTTGATTCAGGGTTCTGGTGGATCATATTGCTTCGTGCTTACACAAAGTCTACCGGAGACTCTTCACTCGCCGAATTGCCTGAATGCCAAAACGGCATTCGCCTCATTCTCAGCCTCTGTCTATCGGAAGGCTTCGATACTTTCCCAACTCTGCTATGTGCTGATGGATGCTGCATGATTGATCGTAGAATGGGTGTGTATGGGTACCCCATAGAAATACAAGCATTATTCTTCATGGCTTTGAGATGTGCTTGCTTTCTTCTCAAGCAAGACGATGTGGGTAAGGAATTTGTAGAGAGGATAACTGATCGTCTCCATGCTTTGAGTTTCCACATGCGGAGCTATTTCTGGCTGGACCTGAAGCAGCTCAATGATATATATCGTTACAAAACGGAGGAGTATTCTCACACAGCAGTGAACAAGTTCAATGTGATGCCCGATTCGCTTCCGGATTGGGTGTTCGATTTCATGCCGAGTCGTGGTGGATACTTCATCGGAAATGTGAGCCCTGCGAGAATGGATTTCCGGTGGTTCTGTTTGGGAAATTGTGTGGCGATTCTGTCGTCTCTGGCAACTCCTGAGCAATCTGCAGCCATAATGGACCTCATTGAAGAACGTTGGGATGAGCTGGTTGGAGAAATGCCTTTGAAAATTTGCTATCCAACTATTGAAGGCCATGAGTATCGAATTGTCACTGGCTGTGACCCTAAGAACACCAAGTGGAGTTACCACAATGGGGGCTCTTGGCCAGTGCTACTATGGTTGTTAACAGCAGCGTGCATAAAGACTGGACGTCCCCTAATTGCAAGGCGTGCAATCGATCTGACTGAAAGCCGGTTATCCAAAGATCACTGGCCGGAGTATTATGACGGAAAGTCAGGGAGGTTCATCGGAAAGCAGGCACGTAAGTTCCAAACGTGGTCTATTGCAGGCTATCTGGTGGCGAAGATGATGATGGATGACCCCTCTCATTTGGGGATGATATCACTAGAGGAAGATAAGCAAATGAAACCCCTTATAAAACGATCGGCTTCATGGACATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

602

Amino Acids

68.62

Weight (kDa)

6.14

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 140 - 575 3.5e-219 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017053)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38450
malus_domestica MD00G1092700.v1.1 MD15G1012100.v1.1
prunus_persica Prupe.1G365400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0302851
rosa_laevigata RLG00000011131
rosa_roxburghii Rroxscaffold_7G00165240
rosa_rugosa Rorug06G0317700
rosa_samantha Rh6AG431000 Rh6BG471700 Rh6CG443400 Rh6DG430100
rosa_wichuraiana Rw6G037340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1778
AatII GACGTC 1 cut(s) 1555
Acc65I GGTACC 1 cut(s) 963
AccB1I GGYRCC 1 cut(s) 963
AccI GTMKAC 3 cut(s) 486, 636, 809
AccII CGCG 1 cut(s) 456
AccIII TCCGGA 1 cut(s) 1207
AciI CCGC 3 cut(s) 433, 456, 1096
AclI AACGTT 1 cut(s) 1375
AclWI GGATC 1 cut(s) 788
AcoI YGGCCR 3 cut(s) 457, 1505, 1613
AcsI RAATTY 2 cut(s) 1046, 1410
AcuI CTGAAG 1 cut(s) 1139
AcyI GRCGYC 1 cut(s) 1552
AdeI CACNNNGTG 1 cut(s) 1479
AfaI GTAC 1 cut(s) 965
AfiI CCNNNNNNNGG 4 cut(s) 420, 622, 1238, 1495
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 8 cut(s) 335, 500, 601, 679, 1186, 1370, 1408, 1430
AhdI GACNNNNNGTC 2 cut(s) 1551, 1633
AjuI GAANNNNNNNTTGG 2 cut(s) 840, 872
AleI CACNNNNGTG 1 cut(s) 1172
AloI GAACNNNNNNTCC 2 cut(s) 1202, 1234
AluBI AGCT 5 cut(s) 649, 675, 1101, 1126, 1387
AluI AGCT 5 cut(s) 649, 675, 1101, 1126, 1387
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 8 cut(s) 3, 165, 256, 419, 525, 810, 1076, 1329
AlwI GGATC 1 cut(s) 788
AlwNI CAGNNNCTG 1 cut(s) 876
Ama87I CYCGRG 2 cut(s) 341, 541
Aor13HI TCCGGA 1 cut(s) 1207
AoxI GGCC 5 cut(s) 457, 552, 1432, 1505, 1613
ApeKI GCWGC 7 cut(s) 209, 216, 479, 930, 1123, 1349, 1532
ApoI RAATTY 2 cut(s) 1046, 1410
Asp700I GAANNNNTTC 1 cut(s) 1181
Asp718I GGTACC 1 cut(s) 963
AspLEI GCGC 3 cut(s) 438, 548, 740
AspS9I GGNCC 3 cut(s) 294, 1114, 1360
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 2 cut(s) 341, 541
AvaII GGWCC 3 cut(s) 294, 1114, 1360
BalI TGGCCA 1 cut(s) 1507
BanI GGYRCC 1 cut(s) 963
BanII GRGCYC 2 cut(s) 1268, 1502
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 7 cut(s) 221, 228, 466, 917, 1135, 1361, 1544
BccI CCATC 3 cut(s) 722, 917, 1708
BceAI ACGGC 4 cut(s) 5, 467, 583, 868
BciVI GTATCC 1 cut(s) 1238
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 8 cut(s) 3, 165, 256, 419, 525, 810, 1076, 1329
BfaI CTAG 3 cut(s) 267, 650, 1748
BfmI CTRYAG 2 cut(s) 720, 1347
BfoI RGCGCY 1 cut(s) 439
BfuI GTATCC 1 cut(s) 1238
BglI GCCNNNNNGGC 1 cut(s) 852
BisI GCNGC 9 cut(s) 210, 217, 434, 457, 480, 931, 1124, 1350, 1533
BlsI GCNGC 9 cut(s) 211, 218, 435, 458, 481, 932, 1125, 1351, 1534
Bme18I GGWCC 3 cut(s) 294, 1114, 1360
BmeRI GACNNNNNGTC 2 cut(s) 1551, 1633
BmeT110I CYCGRG 2 cut(s) 341, 541
BmgT120I GGNCC 3 cut(s) 294, 1114, 1360
BmiI GGNNCC 1 cut(s) 965
BmsI GCATC 5 cut(s) 228, 406, 409, 917, 1182
Bpu10I CCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 2 cut(s) 233, 1007
Bsa29I ATCGAT 1 cut(s) 1578
BsaAI YACGTR 1 cut(s) 1664
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 1552
BsaI GGTCTC 2 cut(s) 256, 419
BsaWI WCCGGW 3 cut(s) 812, 1207, 1284
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 4 cut(s) 420, 622, 1238, 1495
Bse118I RCCGGY 3 cut(s) 212, 463, 1593
Bse1I ACTGG 5 cut(s) 555, 1459, 1508, 1552, 1616
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 1137
BseAI TCCGGA 1 cut(s) 1207
BseCI ATCGAT 1 cut(s) 1578
BseGI GGATG 4 cut(s) 932, 1387, 1723, 1743
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 4 cut(s) 420, 622, 1238, 1495
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 3 cut(s) 128, 883, 1329
BseNI ACTGG 5 cut(s) 555, 1459, 1508, 1552, 1616
BseRI GAGGAG 1 cut(s) 1169
BseXI GCAGC 7 cut(s) 221, 228, 466, 917, 1135, 1361, 1544
Bsh1236I CGCG 1 cut(s) 456
Bsh1285I CGRYCG 1 cut(s) 1787
BshFI GGCC 5 cut(s) 459, 554, 1434, 1507, 1615
BshNI GGYRCC 1 cut(s) 963
BshVI ATCGAT 1 cut(s) 1578
BsiEI CGRYCG 1 cut(s) 1787
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 2 cut(s) 341, 541
BsiSI CCGG 7 cut(s) 213, 464, 813, 1208, 1285, 1594, 1616
BslFI GGGAC 3 cut(s) 484, 524, 1539
BslI CCNNNNNNNGG 4 cut(s) 420, 622, 1238, 1495
BsmAI GTCTC 8 cut(s) 3, 165, 256, 419, 525, 810, 1076, 1329
BsmBI CGTCTC 2 cut(s) 1076, 1329
BsmFI GGGAC 3 cut(s) 484, 524, 1539
BsmI GAATGC 2 cut(s) 848, 855
BsnI GGCC 5 cut(s) 459, 554, 1434, 1507, 1615
Bso31I GGTCTC 2 cut(s) 256, 419
BsoBI CYCGRG 2 cut(s) 341, 541
Bsp1286I GDGCHC 3 cut(s) 690, 1268, 1502
Bsp13I TCCGGA 1 cut(s) 1207
Bsp143I GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
BspACI CCGC 3 cut(s) 433, 456, 1096
BspANI GGCC 5 cut(s) 459, 554, 1434, 1507, 1615
BspCNI CTCAG 3 cut(s) 129, 882, 1330
BspDI ATCGAT 1 cut(s) 1578
BspEI TCCGGA 1 cut(s) 1207
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 965
BspMAI CTGCAG 2 cut(s) 724, 1351
BspPI GGATC 1 cut(s) 788
BspT107I GGYRCC 1 cut(s) 963
BspTNI GGTCTC 2 cut(s) 256, 419
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 3 cut(s) 212, 463, 1593
BsrI ACTGG 5 cut(s) 555, 1459, 1508, 1552, 1616
BssAI RCCGGY 3 cut(s) 212, 463, 1593
BssMI GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
BssNI GRCGYC 1 cut(s) 1552
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 3 cut(s) 284, 742, 826
BstACI GRCGYC 1 cut(s) 1552
BstAPI GCANNNNNTGC 1 cut(s) 1571
BstBAI YACGTR 1 cut(s) 1664
BstC8I GCNNGC 9 cut(s) 214, 461, 669, 673, 1012, 1537, 1572, 1659, 1690
BstDEI CTNAG 6 cut(s) 137, 381, 869, 1338, 1467, 1803
BstF5I GGATG 4 cut(s) 932, 1387, 1723, 1743
BstFNI CGCG 1 cut(s) 456
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 3 cut(s) 438, 548, 740
BstKTI GATC 8 cut(s) 42, 517, 783, 943, 1069, 1582, 1609, 1787
BstMAI GTCTC 8 cut(s) 3, 165, 256, 419, 525, 810, 1076, 1329
BstMBI GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
BstMCI CGRYCG 1 cut(s) 1787
BstMWI GCNNNNNNNGC 3 cut(s) 852, 1201, 1571
BstNSI RCATGY 2 cut(s) 160, 1096
BstSFI CTRYAG 2 cut(s) 720, 1347
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 7 cut(s) 221, 228, 466, 917, 1135, 1361, 1544
BstXI CCANNNNNNTGG 1 cut(s) 467
Bsu15I ATCGAT 1 cut(s) 1578
BsuI GTATCC 1 cut(s) 1238
BsuRI GGCC 5 cut(s) 459, 554, 1434, 1507, 1615
BsuTUI ATCGAT 1 cut(s) 1578
BtsCI GGATG 4 cut(s) 932, 1387, 1723, 1743
BtsI GCAGTG 2 cut(s) 717, 1179
BtsIMutI CAGTG 7 cut(s) 322, 548, 717, 1179, 1452, 1515, 1609
Cac8I GCNNGC 9 cut(s) 214, 461, 669, 673, 1012, 1537, 1572, 1659, 1690
CaiI CAGNNNCTG 1 cut(s) 876
CciI TCATGA 1 cut(s) 202
CfoI GCGC 3 cut(s) 438, 548, 740
Cfr10I RCCGGY 3 cut(s) 212, 463, 1593
Cfr13I GGNCC 3 cut(s) 294, 1114, 1360
ClaI ATCGAT 1 cut(s) 1578
Csp6I GTAC 1 cut(s) 964
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviQI GTAC 1 cut(s) 964
DdeI CTNAG 6 cut(s) 137, 381, 869, 1338, 1467, 1803
DpnI GATC 8 cut(s) 41, 516, 782, 942, 1068, 1581, 1608, 1786
DpnII GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
DraIII CACNNNGTG 1 cut(s) 1479
DriI GACNNNNNGTC 2 cut(s) 1551, 1633
EaeI YGGCCR 3 cut(s) 457, 1505, 1613
Eam1104I CTCTTC 3 cut(s) 284, 742, 826
Eam1105I GACNNNNNGTC 2 cut(s) 1551, 1633
EarI CTCTTC 3 cut(s) 284, 742, 826
Eco24I GRGCYC 2 cut(s) 1268, 1502
Eco31I GGTCTC 2 cut(s) 256, 419
Eco32I GATATC 2 cut(s) 143, 1743
Eco47I GGWCC 3 cut(s) 294, 1114, 1360
Eco57I CTGAAG 1 cut(s) 1139
Eco88I CYCGRG 2 cut(s) 341, 541
EcoO109I RGGNCCY 1 cut(s) 294
EcoRV GATATC 2 cut(s) 143, 1743
EcoT38I GRGCYC 2 cut(s) 1268, 1502
Esp3I CGTCTC 2 cut(s) 1076, 1329
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 3 cut(s) 484, 524, 1539
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 3 cut(s) 486, 636, 809
Fnu4HI GCNGC 9 cut(s) 210, 217, 434, 457, 480, 931, 1124, 1350, 1533
FokI GGATG 4 cut(s) 939, 1394, 1730, 1750
FriOI GRGCYC 2 cut(s) 1268, 1502
Fsp4HI GCNGC 9 cut(s) 210, 217, 434, 457, 480, 931, 1124, 1350, 1533
FspBI CTAG 3 cut(s) 267, 650, 1748
GlaI GCGC 3 cut(s) 437, 547, 739
GluI GCNGC 9 cut(s) 210, 217, 434, 457, 480, 931, 1124, 1350, 1533
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 5 cut(s) 459, 554, 1434, 1507, 1615
HapII CCGG 7 cut(s) 213, 464, 813, 1208, 1285, 1594, 1616
HhaI GCGC 3 cut(s) 438, 548, 740
Hin1I GRCGYC 1 cut(s) 1552
Hin6I GCGC 3 cut(s) 436, 546, 738
HinP1I GCGC 3 cut(s) 436, 546, 738
HincII GTYRAC 3 cut(s) 487, 637, 1527
HindII GTYRAC 3 cut(s) 487, 637, 1527
HinfI GANTC 8 cut(s) 11, 395, 472, 764, 818, 1199, 1234, 1315
HpaI GTTAAC 1 cut(s) 1527
HpaII CCGG 7 cut(s) 213, 464, 813, 1208, 1285, 1594, 1616
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 6 cut(s) 448, 487, 637, 810, 1177, 1527
Hpy188III TCNNGA 6 cut(s) 203, 250, 541, 598, 1208, 1337
Hpy8I GTNNAC 6 cut(s) 448, 487, 637, 810, 1177, 1527
Hpy99I CGWCG 2 cut(s) 488, 641
HpyAV CCTTC 2 cut(s) 881, 1424
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 4 cut(s) 1375, 1552, 1663, 1676
HpyCH4V TGCA 7 cut(s) 722, 933, 1349, 1539, 1565, 1574, 1688
HpyF10VI GCNNNNNNNGC 3 cut(s) 852, 1201, 1571
HpyF3I CTNAG 6 cut(s) 137, 381, 869, 1338, 1467, 1803
HpySE526I ACGT 4 cut(s) 1375, 1552, 1663, 1676
Hsp92I GRCGYC 1 cut(s) 1552
HspAI GCGC 3 cut(s) 436, 546, 738
Kpn2I TCCGGA 1 cut(s) 1207
KpnI GGTACC 1 cut(s) 967
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 1527
Kzo9I GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
LmnI GCTCC 3 cut(s) 653, 760, 1098
Lsp1109I GCAGC 7 cut(s) 221, 228, 466, 917, 1135, 1361, 1544
LweI GCATC 5 cut(s) 228, 406, 409, 917, 1182
MaeI CTAG 3 cut(s) 267, 650, 1748
MaeII ACGT 4 cut(s) 1375, 1552, 1663, 1676
MaeIII GTNAC 5 cut(s) 108, 1142, 1450, 1460, 1484
MalI GATC 8 cut(s) 41, 516, 782, 942, 1068, 1581, 1608, 1786
MboI GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
MhlI GDGCHC 3 cut(s) 690, 1268, 1502
MlsI TGGCCA 1 cut(s) 1507
MluCI AATT 6 cut(s) 833, 1046, 1303, 1410, 1446, 1560
MluNI TGGCCA 1 cut(s) 1507
MlyI GAGTC 5 cut(s) 5, 389, 466, 812, 1243
MmeI TCCRAC 3 cut(s) 188, 1372, 1445
Mox20I TGGCCA 1 cut(s) 1507
MroI TCCGGA 1 cut(s) 1207
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 1181
MscI TGGCCA 1 cut(s) 1507
MseI TTAA 3 cut(s) 507, 645, 1526
MslI CAYNNNNRTG 1 cut(s) 1172
Msp20I TGGCCA 1 cut(s) 1507
MspI CCGG 7 cut(s) 213, 464, 813, 1208, 1285, 1594, 1616
Mva1269I GAATGC 2 cut(s) 848, 855
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 852, 1201, 1571
NaeI GCCGGC 1 cut(s) 214
NdeII GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
NgoMIV GCCGGC 1 cut(s) 212
NlaIV GGNNCC 1 cut(s) 965
NmeAIII GCCGAG 2 cut(s) 202, 1257
NmuCI GTSAC 2 cut(s) 1450, 1460
NspI RCATGY 2 cut(s) 160, 1096
OliI CACNNNNGTG 1 cut(s) 1172
PaeR7I CTCGAG 1 cut(s) 341
PagI TCATGA 1 cut(s) 202
PciI ACATGT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 2 cut(s) 848, 855
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 1181
PfeI GAWTC 3 cut(s) 764, 1199, 1315
PflFI GACNNNGTC 2 cut(s) 536, 695
PkrI GCNGC 9 cut(s) 211, 218, 435, 458, 481, 932, 1125, 1351, 1534
Ple19I CGATCG 1 cut(s) 1787
PleI GAGTC 5 cut(s) 5, 389, 466, 812, 1242
PpsI GAGTC 5 cut(s) 5, 389, 466, 812, 1242
Ppu21I YACGTR 1 cut(s) 1664
PpuMI RGGWCCY 1 cut(s) 294
PscI ACATGT 1 cut(s) 156
PsiI TTATAA 1 cut(s) 1778
Psp1406I AACGTT 1 cut(s) 1375
Psp5II RGGWCCY 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 965
PspPI GGNCC 3 cut(s) 294, 1114, 1360
PspPPI RGGWCCY 1 cut(s) 294
PspXI VCTCGAGB 1 cut(s) 341
PstI CTGCAG 2 cut(s) 724, 1351
PstNI CAGNNNCTG 1 cut(s) 876
PsyI GACNNNGTC 2 cut(s) 536, 695
PvuI CGATCG 1 cut(s) 1787
RsaI GTAC 1 cut(s) 965
RsaNI GTAC 1 cut(s) 964
RseI CAYNNNNRTG 1 cut(s) 1172
SalI GTCGAC 2 cut(s) 485, 635
SaqAI TTAA 3 cut(s) 507, 645, 1526
SatI GCNGC 9 cut(s) 210, 217, 434, 457, 480, 931, 1124, 1350, 1533
Sau3AI GATC 8 cut(s) 39, 514, 780, 940, 1066, 1579, 1606, 1784
Sau96I GGNCC 3 cut(s) 294, 1114, 1360
SchI GAGTC 5 cut(s) 5, 389, 466, 812, 1243
SduI GDGCHC 3 cut(s) 690, 1268, 1502
SfaNI GCATC 5 cut(s) 228, 406, 409, 917, 1182
SfcI CTRYAG 2 cut(s) 720, 1347
Sfr274I CTCGAG 1 cut(s) 341
SinI GGWCC 3 cut(s) 294, 1114, 1360
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 1172
SmlI CTYRAG 3 cut(s) 248, 341, 1022
SmoI CTYRAG 3 cut(s) 248, 341, 1022
Sse9I AATT 6 cut(s) 833, 1046, 1303, 1410, 1446, 1560
SsiI CCGC 3 cut(s) 433, 456, 1096
SspMI CTAG 3 cut(s) 267, 650, 1748
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 4 cut(s) 1378, 1555, 1666, 1679
TaqI TCGA 8 cut(s) 153, 342, 486, 636, 894, 1221, 1444, 1578
TasI AATT 6 cut(s) 833, 1046, 1303, 1410, 1446, 1560
TauI GCSGC 2 cut(s) 436, 459
TfiI GAWTC 3 cut(s) 764, 1199, 1315
Tru1I TTAA 3 cut(s) 507, 645, 1526
Tru9I TTAA 3 cut(s) 507, 645, 1526
TscAI CASTG 7 cut(s) 322, 555, 724, 1179, 1459, 1515, 1616
TseFI GTSAC 2 cut(s) 1450, 1460
TseI GCWGC 7 cut(s) 209, 216, 479, 930, 1123, 1349, 1532
Tsp45I GTSAC 2 cut(s) 1450, 1460
TspDTI ATGAA 9 cut(s) 72, 318, 578, 982, 1216, 1240, 1636, 1781, 1782
TspGWI ACGGA 2 cut(s) 1166, 1644
TspRI CASTG 7 cut(s) 322, 555, 724, 1179, 1459, 1515, 1616
Tth111I GACNNNGTC 2 cut(s) 536, 695
VpaK11BI GGWCC 3 cut(s) 294, 1114, 1360
XapI RAATTY 2 cut(s) 1046, 1410
XceI RCATGY 2 cut(s) 160, 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 1515
XhoI CTCGAG 1 cut(s) 341
XmiI GTMKAC 3 cut(s) 486, 636, 809
XmnI GAANNNNTTC 1 cut(s) 1181
XspI CTAG 3 cut(s) 267, 650, 1748
ZraI GACGTC 1 cut(s) 1553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.