MD15G1012100.v1.1

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017053
Physical Location & Seq
Forward (+)
677384 .. 679595
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1012100.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGTCTCCAGTTCATGGCGATCATGCTTCACAAAATGGGAATACAAGAACTCAAGAGACAGTCGTTTTTGAAATTGGAGAAGACTCCGATTTTCTGAGGTTACTGGACAGGCCTAGACCAGTAAGTGTGGAGAAGAAGAGATCCTTCGATGAAGGGTCGTTCAGCGAGTTGTCCAGTATAGGGTCTCCTTGTAGAAGACCCAGTTTTCTGGGGAGTCCTAGACTGAACTATGAGTCACATCCCGTGGTTTCCGATGCCTGGGAAACCCTGCGACGCTCTGTCGTGCACTTCCGGGGCCAGCCTGTTGGAACCCTTGCCGCTGTCGACCATTCTGTCGAAGAACTTAACTATGATCAGGTGTTTGTTAGAGATTTTGTGCCAAGCGCATTGGCATTTTTGATGAATGGAGAGGAGGAGATAGTGAAAAACTTTCTTTTGAAAACACTTCGCCTCCAATCATTGGAGAAAATTGTGGACCAGTTCAAGCTAGGAGAAGGTGTCATGCCGGCGAGCTTCAAAGTGCTCCATGACCCCGTAAGAAACTTCGAAACCATAACAGCGGATTTTGGGGAGAGCGCAATTGGAAGAGTAGCTCCGGTTGATTCTGGATTTTGGTGGATAATATTGCTTCGCGCGTACACAAAGTCTACCGGGGACACTTCGGTCTCCGAAATGCCCGAATGCCAAAACGGCATTCGCCTCATCCTGGATCTCTGCCTCTCGGAAGGATTTGATACTTTCCCTACTCTGCTCTGTGCTGATGGATGCTGTATGATTGATCGTAGAATGGGTGTGTATGGATATCCAATCGAAATTCAAGCACTGTTCTTCATGGCATTACGATGCGCTCTGGTTCTGCTCAAGCAAGACAACTGCGGCAAAGAATTCGTGAAGCGCATAACCGACCGCCTCCATGCCCTGAGCTACCACATGCGCAGCTACTTCTGGCTGGACCTGAAGCAGCTCAACGACATCTACCGTTACAAGACGGAGGAGTACTCGCACACGGCGGTAAACAAGTTCAACGTCATGCCGGATTCGCTACCATATTGGGTGTTCGATTTCATGCCGAGTCGCGGCGGTTACTTCATCGGCAACGTGAGCCCGGCCAGGATGGATTTCCGGTGGTTCTGTTTGGGCAATTGCGTGGCGATTTTATCGTCGTTGGCTACGCCGGAGCAGGCGTCGGCGATCATGGATCTTATTGAGTCACGGTGGGAAGAGTTGGTCGGAGAAATGCCGTTGAAGATTTGCTATCCGGCATTTGAAAGTCATGAGTGGAGGATTGTTACTGGCTGTGATCCAAAGAATACTAGATGGAGTTACCACAATGGAGGCTCTTGGCCAGTACTGCTGTGGTTGTTAACCGCGGCATGCATAAAGACCGGCCGGCCACAGATTGCAAGGCGTGCGATCGAGCTGGCGGAAAGCCGGTTGTCGAAAGATGGGTGGCCGGAGTACTATGATGGGAAGCTAGGGAGGTTCATGGGGAAGCAGGCACGGAAGTACCAGACATGGTCGATCGCGGGGTACTTGGTGGCGAAGATGATGCTGGAAGACCCGTCTCATTTGGGCATGATAGCATTGGAGGAGGACGAGCAAATGAAACCCCTTATTAAAAGATCTGCTTCATGGACCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

548

Amino Acids

62.37

Weight (kDa)

5.92

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 86 - 521 5.5e-219 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017053)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38450
malus_domestica MD00G1092700.v1.1 MD15G1012100.v1.1
prunus_persica Prupe.1G365400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0302851
rosa_laevigata RLG00000011131
rosa_roxburghii Rroxscaffold_7G00165240
rosa_rugosa Rorug06G0317700
rosa_samantha Rh6AG431000 Rh6BG471700 Rh6CG443400 Rh6DG430100
rosa_wichuraiana Rw6G037340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 332, 662
Acc16I TGCGCA 1 cut(s) 935
AccB7I CCANNNNNTGG 2 cut(s) 14, 460
AccI GTMKAC 2 cut(s) 324, 647
AccII CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
AclWI GGATC 4 cut(s) 135, 717, 1206, 1295
AcoI YGGCCR 5 cut(s) 1107, 1343, 1387, 1391, 1451
AcsI RAATTY 2 cut(s) 813, 884
AcuI CTGAAG 1 cut(s) 977
AcyI GRCGYC 1 cut(s) 1184
AfaI GTAC 6 cut(s) 638, 998, 1350, 1460, 1508, 1532
AfiI CCNNNNNNNGG 6 cut(s) 14, 180, 258, 460, 706, 1076
AgsI TTSAA 8 cut(s) 71, 439, 484, 517, 818, 1024, 1246, 1268
AhdI GACNNNNNGTC 1 cut(s) 278
AjnI CCWGG 3 cut(s) 257, 705, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AluBI AGCT 8 cut(s) 487, 513, 593, 924, 939, 964, 1420, 1474
AluI AGCT 8 cut(s) 487, 513, 593, 924, 939, 964, 1420, 1474
Alw21I GWGCWC 2 cut(s) 288, 525
Alw26I GTCTC 5 cut(s) 9, 50, 189, 670, 1569
Alw44I GTGCAC 1 cut(s) 284
AlwI GGATC 4 cut(s) 135, 717, 1206, 1295
AoxI GGCC 7 cut(s) 110, 295, 1107, 1343, 1387, 1391, 1451
ApaLI GTGCAC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 936, 961
ApoI RAATTY 2 cut(s) 813, 884
AspLEI GCGC 6 cut(s) 386, 578, 635, 848, 897, 936
AspS9I GGNCC 4 cut(s) 295, 475, 952, 1635
AsuC2I CCSGG 3 cut(s) 293, 652, 1106
AsuII TTCGAA 1 cut(s) 546
AvaII GGWCC 3 cut(s) 475, 952, 1635
BaeGI GKGCMC 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 1490, 1523
BalI TGGCCA 1 cut(s) 1345
BanII GRGCYC 1 cut(s) 1106
BbsI GAAGAC 3 cut(s) 87, 202, 1563
Bbv12I GWGCWC 2 cut(s) 288, 525
BbvI GCAGC 2 cut(s) 948, 973
BccI CCATC 5 cut(s) 755, 1108, 1311, 1439, 1460
BceAI ACGGC 3 cut(s) 706, 1023, 1225
BcgI CGANNNNNNTGC 4 cut(s) 868, 902, 1531, 1565
BciT130I CCWGG 3 cut(s) 259, 707, 1111
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 3 cut(s) 293, 652, 1106
BcoDI GTCTC 5 cut(s) 9, 50, 189, 670, 1569
BfaI CTAG 5 cut(s) 114, 219, 488, 1314, 1475
BglI GCCNNNNNGGC 1 cut(s) 690
BglII AGATCT 1 cut(s) 1622
BisI GCNGC 6 cut(s) 318, 877, 937, 962, 1078, 1371
BlsI GCNGC 6 cut(s) 319, 878, 938, 963, 1079, 1372
BmcAI AGTACT 3 cut(s) 998, 1350, 1460
Bme1390I CCNGG 6 cut(s) 259, 293, 652, 707, 1106, 1111
Bme18I GGWCC 3 cut(s) 475, 952, 1635
BmeRI GACNNNNNGTC 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 295, 475, 952, 1635
BmiI GGNNCC 2 cut(s) 296, 310
BmrFI CCNGG 6 cut(s) 259, 293, 652, 707, 1106, 1111
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 244, 755, 833, 1539
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 3 cut(s) 87, 202, 1563
BplI GAGNNNNNCTC 2 cut(s) 983, 1015
Bpu10I CCTNAGC 1 cut(s) 920
Bpu14I TTCGAA 1 cut(s) 546
BpuEI CTTGAG 2 cut(s) 36, 845
BpuMI CCSGG 3 cut(s) 293, 652, 1106
BsaHI GRCGYC 1 cut(s) 1184
BsaI GGTCTC 2 cut(s) 189, 670
BsaJI CCNNGG 5 cut(s) 243, 258, 292, 651, 1368
BsaWI WCCGGW 2 cut(s) 595, 1122
BsaXI ACNNNNNCTCC 6 cut(s) 435, 465, 483, 513, 1169, 1199
Bsc4I CCNNNNNNNGG 6 cut(s) 14, 180, 258, 460, 706, 1076
Bse118I RCCGGY 4 cut(s) 505, 1385, 1389, 1431
Bse1I ACTGG 8 cut(s) 8, 108, 119, 174, 201, 478, 1297, 1346
BseBI CCWGG 3 cut(s) 259, 707, 1111
BseDI CCNNGG 5 cut(s) 243, 258, 292, 651, 1368
BseGI GGATG 4 cut(s) 238, 702, 770, 1119
BseLI CCNNNNNNNGG 6 cut(s) 14, 180, 258, 460, 706, 1076
BseMII CTCAG 2 cut(s) 86, 911
BseNI ACTGG 8 cut(s) 8, 108, 119, 174, 201, 478, 1297, 1346
BseRI GAGGAG 4 cut(s) 425, 428, 1007, 1604
BseSI GKGCMC 1 cut(s) 288
BseX3I CGGCCG 1 cut(s) 1387
BseXI GCAGC 2 cut(s) 948, 973
Bsh1236I CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
Bsh1285I CGRYCG 4 cut(s) 907, 1390, 1416, 1524
BshFI GGCC 7 cut(s) 112, 297, 1109, 1345, 1389, 1393, 1453
BsiEI CGRYCG 4 cut(s) 907, 1390, 1416, 1524
BsiHKAI GWGCWC 2 cut(s) 288, 525
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 6 cut(s) 14, 180, 258, 460, 706, 1076
BsmAI GTCTC 5 cut(s) 9, 50, 189, 670, 1569
BsmBI CGTCTC 1 cut(s) 1569
BsmFI GGGAC 1 cut(s) 668
BsmI GAATGC 2 cut(s) 686, 693
BsnI GGCC 7 cut(s) 112, 297, 1109, 1345, 1389, 1393, 1453
Bso31I GGTCTC 2 cut(s) 189, 670
Bsp119I TTCGAA 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 288, 525, 1106
BspANI GGCC 7 cut(s) 112, 297, 1109, 1345, 1389, 1393, 1453
BspCNI CTCAG 2 cut(s) 87, 912
BspFNI CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
BspHI TCATGA 1 cut(s) 1273
BspLI GGNNCC 2 cut(s) 296, 310
BspPI GGATC 4 cut(s) 135, 717, 1206, 1295
BspT104I TTCGAA 1 cut(s) 546
BspTNI GGTCTC 2 cut(s) 189, 670
BsrFI RCCGGY 4 cut(s) 505, 1385, 1389, 1431
BsrI ACTGG 8 cut(s) 8, 108, 119, 174, 201, 478, 1297, 1346
BssAI RCCGGY 4 cut(s) 505, 1385, 1389, 1431
BssECI CCNNGG 5 cut(s) 243, 258, 292, 651, 1368
BssNI GRCGYC 1 cut(s) 1184
Bst2UI CCWGG 3 cut(s) 259, 707, 1111
Bst4CI ACNGT 4 cut(s) 61, 825, 980, 1215
Bst6I CTCTTC 3 cut(s) 131, 580, 1215
BstACI GRCGYC 1 cut(s) 1184
BstAPI GCANNNNNTGC 1 cut(s) 1409
BstBI TTCGAA 1 cut(s) 546
BstC8I GCNNGC 9 cut(s) 299, 507, 511, 1182, 1375, 1391, 1410, 1422, 1497
BstDEI CTNAG 2 cut(s) 95, 920
BstDSI CCRYGG 2 cut(s) 243, 1368
BstF5I GGATG 4 cut(s) 238, 702, 770, 1119
BstFNI CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
BstHHI GCGC 6 cut(s) 386, 578, 635, 848, 897, 936
BstMAI GTCTC 5 cut(s) 9, 50, 189, 670, 1569
BstMCI CGRYCG 4 cut(s) 907, 1390, 1416, 1524
BstMWI GCNNNNNNNGC 5 cut(s) 690, 1039, 1101, 1351, 1409
BstNI CCWGG 3 cut(s) 259, 707, 1111
BstNSI RCATGY 2 cut(s) 934, 1377
BstSCI CCNGG 6 cut(s) 257, 291, 650, 705, 1104, 1109
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
BstV1I GCAGC 2 cut(s) 948, 973
BstV2I GAAGAC 3 cut(s) 87, 202, 1563
BstX2I RGATCY 4 cut(s) 140, 709, 1198, 1622
BstXI CCANNNNNNTGG 2 cut(s) 208, 305
BstYI RGATCY 4 cut(s) 140, 709, 1198, 1622
BstZI CGGCCG 1 cut(s) 1387
BsuRI GGCC 7 cut(s) 112, 297, 1109, 1345, 1389, 1393, 1453
BtgI CCRYGG 2 cut(s) 243, 1368
BtsCI GGATG 4 cut(s) 238, 702, 770, 1119
BtsIMutI CAGTG 1 cut(s) 821
Cac8I GCNNGC 9 cut(s) 299, 507, 511, 1182, 1375, 1391, 1410, 1422, 1497
CciI TCATGA 1 cut(s) 1273
CfoI GCGC 6 cut(s) 386, 578, 635, 848, 897, 936
Cfr10I RCCGGY 4 cut(s) 505, 1385, 1389, 1431
Cfr13I GGNCC 4 cut(s) 295, 475, 952, 1635
Cfr42I CCGCGG 1 cut(s) 1371
CseI GACGC 2 cut(s) 282, 1173
Csp6I GTAC 6 cut(s) 637, 997, 1349, 1459, 1507, 1531
CviQI GTAC 6 cut(s) 637, 997, 1349, 1459, 1507, 1531
DdeI CTNAG 2 cut(s) 95, 920
DrdI GACNNNNNNGTC 2 cut(s) 332, 662
DriI GACNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 2 cut(s) 332, 662
EaeI YGGCCR 5 cut(s) 1107, 1343, 1387, 1391, 1451
EagI CGGCCG 1 cut(s) 1387
Eam1104I CTCTTC 3 cut(s) 131, 580, 1215
Eam1105I GACNNNNNGTC 1 cut(s) 278
EarI CTCTTC 3 cut(s) 131, 580, 1215
EciI GGCGGA 1 cut(s) 1439
EclXI CGGCCG 1 cut(s) 1387
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 1106
Eco31I GGTCTC 2 cut(s) 189, 670
Eco32I GATATC 1 cut(s) 803
Eco47I GGWCC 3 cut(s) 475, 952, 1635
Eco52I CGGCCG 1 cut(s) 1387
Eco57I CTGAAG 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 884
EcoRII CCWGG 3 cut(s) 257, 705, 1109
EcoRV GATATC 1 cut(s) 803
EcoT22I ATGCAT 1 cut(s) 1379
EcoT38I GRGCYC 1 cut(s) 1106
Esp3I CGTCTC 1 cut(s) 1569
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 1612, 1644
FaqI GGGAC 1 cut(s) 668
FauI CCCGC 1 cut(s) 1519
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 2 cut(s) 324, 647
Fnu4HI GCNGC 6 cut(s) 318, 877, 937, 962, 1078, 1371
FokI GGATG 4 cut(s) 225, 689, 777, 1126
FriOI GRGCYC 1 cut(s) 1106
FseI GGCCGGCC 1 cut(s) 1393
Fsp4HI GCNGC 6 cut(s) 318, 877, 937, 962, 1078, 1371
FspBI CTAG 5 cut(s) 114, 219, 488, 1314, 1475
FspI TGCGCA 1 cut(s) 935
GlaI GCGC 6 cut(s) 385, 577, 634, 847, 896, 935
GluI GCNGC 6 cut(s) 318, 877, 937, 962, 1078, 1371
HaeIII GGCC 7 cut(s) 112, 297, 1109, 1345, 1389, 1393, 1453
HgaI GACGC 2 cut(s) 282, 1173
HhaI GCGC 6 cut(s) 386, 578, 635, 848, 897, 936
Hin1I GRCGYC 1 cut(s) 1184
Hin6I GCGC 6 cut(s) 384, 576, 633, 846, 895, 934
HinP1I GCGC 6 cut(s) 384, 576, 633, 846, 895, 934
HincII GTYRAC 2 cut(s) 325, 1365
HindII GTYRAC 2 cut(s) 325, 1365
HinfI GANTC 7 cut(s) 83, 214, 233, 602, 1037, 1072, 1208
HpaI GTTAAC 1 cut(s) 1365
Hpy166II GTNNAC 7 cut(s) 286, 325, 475, 639, 648, 1015, 1365
Hpy188I TCNGA 6 cut(s) 88, 96, 253, 670, 724, 1232
Hpy188III TCNNGA 4 cut(s) 53, 606, 889, 1274
Hpy8I GTNNAC 7 cut(s) 286, 325, 475, 639, 648, 1015, 1365
Hpy99I CGWCG 3 cut(s) 276, 1165, 1189
HpyAV CCTTC 4 cut(s) 146, 154, 488, 719
HpyCH4III ACNGT 4 cut(s) 61, 825, 980, 1215
HpyCH4IV ACGT 2 cut(s) 1026, 1098
HpyCH4V TGCA 3 cut(s) 286, 1377, 1403
HpyF10VI GCNNNNNNNGC 5 cut(s) 690, 1039, 1101, 1351, 1409
HpyF3I CTNAG 2 cut(s) 95, 920
HpySE526I ACGT 2 cut(s) 1026, 1098
Hsp92I GRCGYC 1 cut(s) 1184
HspAI GCGC 6 cut(s) 384, 576, 633, 846, 895, 934
KroI GCCGGC 2 cut(s) 505, 1389
KroNI GCCGGC 2 cut(s) 507, 1391
Ksp22I TGATCA 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 1365
KspI CCGCGG 1 cut(s) 1371
LmnI GCTCC 3 cut(s) 528, 598, 1177
Lsp1109I GCAGC 2 cut(s) 948, 973
LweI GCATC 4 cut(s) 244, 755, 833, 1539
MaeI CTAG 5 cut(s) 114, 219, 488, 1314, 1475
MaeII ACGT 2 cut(s) 1026, 1098
MaeIII GTNAC 7 cut(s) 99, 234, 980, 1082, 1209, 1288, 1322
MfeI CAATTG 2 cut(s) 579, 1141
MflI RGATCY 4 cut(s) 140, 709, 1198, 1622
MhlI GDGCHC 3 cut(s) 288, 525, 1106
MlsI TGGCCA 1 cut(s) 1345
MluCI AATT 6 cut(s) 72, 468, 579, 813, 884, 1141
MluNI TGGCCA 1 cut(s) 1345
MlyI GAGTC 5 cut(s) 77, 223, 242, 1081, 1217
MmeI TCCRAC 2 cut(s) 286, 1210
Mox20I TGGCCA 1 cut(s) 1345
Mph1103I ATGCAT 1 cut(s) 1379
MroNI GCCGGC 2 cut(s) 505, 1389
MscI TGGCCA 1 cut(s) 1345
MseI TTAA 4 cut(s) 345, 1364, 1617, 1642
MslI CAYNNNNRTG 1 cut(s) 841
Msp20I TGGCCA 1 cut(s) 1345
MspA1I CMGCKG 3 cut(s) 320, 560, 1370
MspR9I CCNGG 6 cut(s) 259, 293, 652, 707, 1106, 1111
MunI CAATTG 2 cut(s) 579, 1141
Mva1269I GAATGC 2 cut(s) 686, 693
MvaI CCWGG 3 cut(s) 259, 707, 1111
MvnI CGCG 5 cut(s) 633, 635, 1077, 1370, 1526
MwoI GCNNNNNNNGC 5 cut(s) 690, 1039, 1101, 1351, 1409
NaeI GCCGGC 2 cut(s) 507, 1391
NciI CCSGG 3 cut(s) 293, 652, 1106
NgoMIV GCCGGC 2 cut(s) 505, 1389
NlaIV GGNNCC 2 cut(s) 296, 310
NmeAIII GCCGAG 1 cut(s) 1095
NmuCI GTSAC 2 cut(s) 234, 1209
NsbI TGCGCA 1 cut(s) 935
NsiI ATGCAT 1 cut(s) 1379
NspI RCATGY 2 cut(s) 934, 1377
NspV TTCGAA 1 cut(s) 546
PaeI GCATGC 1 cut(s) 1377
PagI TCATGA 1 cut(s) 1273
PceI AGGCCT 1 cut(s) 112
PctI GAATGC 2 cut(s) 686, 693
PdiI GCCGGC 2 cut(s) 507, 1391
PfeI GAWTC 2 cut(s) 602, 1037
PflFI GACNNNGTC 1 cut(s) 1516
PflMI CCANNNNNTGG 2 cut(s) 14, 460
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 6 cut(s) 319, 878, 938, 963, 1079, 1372
Ple19I CGATCG 2 cut(s) 1416, 1524
PleI GAGTC 5 cut(s) 77, 222, 241, 1080, 1216
PpsI GAGTC 5 cut(s) 77, 222, 241, 1080, 1216
Psp6I CCWGG 3 cut(s) 257, 705, 1109
PspGI CCWGG 3 cut(s) 257, 705, 1109
PspN4I GGNNCC 2 cut(s) 296, 310
PspPI GGNCC 4 cut(s) 295, 475, 952, 1635
PsuI RGATCY 4 cut(s) 140, 709, 1198, 1622
PsyI GACNNNGTC 1 cut(s) 1516
PvuI CGATCG 2 cut(s) 1416, 1524
RigI GGCCGGCC 1 cut(s) 1393
RsaI GTAC 6 cut(s) 638, 998, 1350, 1460, 1508, 1532
RsaNI GTAC 6 cut(s) 637, 997, 1349, 1459, 1507, 1531
RseI CAYNNNNRTG 1 cut(s) 841
SacII CCGCGG 1 cut(s) 1371
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 4 cut(s) 345, 1364, 1617, 1642
SatI GCNGC 6 cut(s) 318, 877, 937, 962, 1078, 1371
Sau96I GGNCC 4 cut(s) 295, 475, 952, 1635
ScaI AGTACT 3 cut(s) 998, 1350, 1460
SchI GAGTC 5 cut(s) 77, 223, 242, 1081, 1217
ScrFI CCNGG 6 cut(s) 259, 293, 652, 707, 1106, 1111
SduI GDGCHC 3 cut(s) 288, 525, 1106
SfaNI GCATC 4 cut(s) 244, 755, 833, 1539
Sfr303I CCGCGG 1 cut(s) 1371
SfuI TTCGAA 1 cut(s) 546
SgrBI CCGCGG 1 cut(s) 1371
SinI GGWCC 3 cut(s) 475, 952, 1635
SmiMI CAYNNNNRTG 1 cut(s) 841
SmlI CTYRAG 2 cut(s) 51, 860
SmoI CTYRAG 2 cut(s) 51, 860
SphI GCATGC 1 cut(s) 1377
Sse9I AATT 6 cut(s) 72, 468, 579, 813, 884, 1141
SseBI AGGCCT 1 cut(s) 112
SspI AATATT 1 cut(s) 624
SspMI CTAG 5 cut(s) 114, 219, 488, 1314, 1475
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 6 cut(s) 257, 291, 650, 705, 1104, 1109
TaaI ACNGT 4 cut(s) 61, 825, 980, 1215
TaiI ACGT 2 cut(s) 1029, 1101
TaqI TCGA 9 cut(s) 147, 324, 336, 546, 810, 1059, 1416, 1439, 1520
TaqII GACCGA 1 cut(s) 652
TasI AATT 6 cut(s) 72, 468, 579, 813, 884, 1141
TatI WGTACW 3 cut(s) 996, 1348, 1458
TauI GCSGC 4 cut(s) 320, 879, 1080, 1373
TfiI GAWTC 2 cut(s) 602, 1037
Tru1I TTAA 4 cut(s) 345, 1364, 1617, 1642
Tru9I TTAA 4 cut(s) 345, 1364, 1617, 1642
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 2 cut(s) 234, 1209
TseI GCWGC 2 cut(s) 936, 961
Tsp45I GTSAC 2 cut(s) 234, 1209
TspDTI ATGAA 8 cut(s) 165, 416, 820, 1054, 1078, 1474, 1619, 1620
TspGWI ACGGA 2 cut(s) 1004, 1516
TspRI CASTG 1 cut(s) 828
Tth111I GACNNNGTC 1 cut(s) 1516
Van91I CCANNNNNTGG 2 cut(s) 14, 460
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 3 cut(s) 475, 952, 1635
XapI RAATTY 2 cut(s) 813, 884
XceI RCATGY 2 cut(s) 934, 1377
XcmI CCANNNNNNNNNTGG 1 cut(s) 1353
XmiI GTMKAC 2 cut(s) 324, 647
XspI CTAG 5 cut(s) 114, 219, 488, 1314, 1475
ZrmI AGTACT 3 cut(s) 998, 1350, 1460
Zsp2I ATGCAT 1 cut(s) 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.