MD00G1092700.v1.1

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017053
Physical Location & Seq
Reverse (-)
19548773 .. 19551452
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1092700.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGTATCCAGTTAATGGGGATCATGCTTCACAAAATGGGAATGCGAGAACTCACGAGCCGCCTGTTTTCGAAATCGGAGAAGACTACGATTTTCTGAGGTTGCTGGACAGGCCTAGACCGGTAAGCGTGGAGAAGAAGAGATCATTCGATGAAAGGTCGTTCAGCGAGTTGTCTTTTTTGGGGAGTCCTAGACCAAGCTTTGACACCAATCCCATGGTTTCCGATGCCTGGGAAACCTTGCGCCGCTCTGTAGTTCACTTCCGTGGCCAGCCTGTTGGAACCCTTGCCGCCGTCGACCATTCGGTCGAAGAACTTAACTATGATCAGGTGTTTGTTAGAGATTTTGTGCCAAGTGCATTGGCATTTTTGATGAATGGAGAATATGAGATAGTGAAGAACTTTCTTTTGAAAACACTTCGCCTGCAATCACTGAAGAAATTTGTGGACCAATTCAAGCTAGGAGAAGGGGTCATGCCGGCGAGCTTCAAAGTGCACCATGACCCCATACGAAACTACGAAACCATAACTGCGGATTTTGGCGAGACCGCAATCGGAAGAGTGGCTCCGGTCGATTCTGGATTCTGGTGGATCATATTGCTTCGAGCTTACACAAAGTCGACCGGGGACTCTTCGGTCTCCGAAATGCCCGATTGCCAAAACGGCATTCGCCTCATCCTCGATCTCTGCCTCTCAGAAGGATTTGATACCTTCCCTACCCTGCTCTGTGCTGATGGATGTTGTATGATTGATCGCAGAATGGGTGTTTACGGATATCCAATCGAAATTCAAGCACTGTTCTTCATGGCATTACGATGCGCTCTGGTTCTGCTCAAGCAAGACAACTGCGGCAAAGAATTCGTAAACCGCATAACGGACCGCCTCCACGCCCTGAGCTACCACATGCGCAGCTACTTCTGGCTGGACCTGAAGCAGCTCAACGACATCTACCGCTACAAGACGGAGGAGTACTCTCATACGGCAGTGAACAAGTTCAACGTCATGCCGGATTCGCTGCCCAATTGGGTGTTCGATTTCATGCCGAGCCGCGGCGGTTACTTCATTGGGAATGTGAGCCCGGCCAGAATGGATTTCCGGTGGTTCTGTTTGGGCAATTGCATGGCGATTTTATCGTCTTTGGCGACGCCGGAGCAGGCATCGGCGATCATGGATCTCATTGAGTCGCGGTGGGATGAGTTGGTCGGAGAAGTGCCGTTGAAGATTTGCTATCCTGCAATAGAAAGTCATGAGTGGAGGATTGTTACTGGCTGTGATCCAAAGAATACTAGGTGGAGTTACCACAATGGAGGCTCTTGGCCAGTGCTGCTGTGGCTGTTGACGGCGGCATGCATAAAGACGGGGAGGCCCCAAATAGCGCGACGTGCGATCGAGCTGGCGGAGAGCCGGTTGTCGAAAGACGGCTGGCCGGAGTACTATGACGGGAAGCTAGGGAAGTACATGGGAAAGCAGGCGCGGAAGTACCAGACATGGTCGATTGCCGGGTACTTGGTGTCGAAGATGATGCTGGAAGACCCGTCTCATTTGGGCATGGTAGCATTGGAGGAGGACGAGCAAATGAAACCCCTAATAAAAAGATCTGCTTCATGGACCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

538

Amino Acids

61.46

Weight (kDa)

6.0

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 76 - 511 1.9e-218 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017053)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38450
malus_domestica MD00G1092700.v1.1 MD15G1012100.v1.1
prunus_persica Prupe.1G365400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0302851
rosa_laevigata RLG00000011131
rosa_roxburghii Rroxscaffold_7G00165240
rosa_rugosa Rorug06G0317700
rosa_samantha Rh6AG431000 Rh6BG471700 Rh6CG443400 Rh6DG430100
rosa_wichuraiana Rw6G037340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 302, 632
Acc16I TGCGCA 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 14
AccBSI CCGCTC 1 cut(s) 246
AccI GTMKAC 2 cut(s) 294, 617
AccII CGCG 4 cut(s) 1047, 1183, 1375, 1471
AclWI GGATC 4 cut(s) 27, 596, 1176, 1265
AcoI YGGCCR 4 cut(s) 265, 1077, 1313, 1421
AcsI RAATTY 3 cut(s) 437, 783, 854
AcuI CTGAAG 2 cut(s) 452, 947
AcyI GRCGYC 1 cut(s) 1142
AfaI GTAC 5 cut(s) 968, 1430, 1454, 1478, 1502
AfiI CCNNNNNNNGG 4 cut(s) 14, 228, 871, 1046
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 6 cut(s) 409, 454, 487, 788, 994, 1216
AjiI CACGTC 1 cut(s) 1379
AjnI CCWGG 1 cut(s) 227
AjuI GAANNNNNNNTTGG 4 cut(s) 648, 680, 1010, 1042
AleI CACNNNNGTG 1 cut(s) 261
AluBI AGCT 9 cut(s) 198, 457, 483, 605, 894, 909, 934, 1390, 1444
AluI AGCT 9 cut(s) 198, 457, 483, 605, 894, 909, 934, 1390, 1444
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 3 cut(s) 536, 640, 1539
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 4 cut(s) 27, 596, 1176, 1265
AoxI GGCC 6 cut(s) 110, 265, 1077, 1313, 1361, 1421
ApaLI GTGCAC 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 906, 931, 1012, 1321
ApoI RAATTY 3 cut(s) 437, 783, 854
AsiGI ACCGGT 1 cut(s) 118
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 5 cut(s) 243, 818, 906, 1375, 1471
AspS9I GGNCC 5 cut(s) 445, 874, 922, 1362, 1605
AsuC2I CCSGG 3 cut(s) 622, 1076, 1498
AsuII TTCGAA 1 cut(s) 69
AvaII GGWCC 4 cut(s) 445, 874, 922, 1605
BaeGI GKGCMC 1 cut(s) 495
BalI TGGCCA 2 cut(s) 267, 1315
BanII GRGCYC 1 cut(s) 1076
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 87, 1533
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 4 cut(s) 918, 943, 999, 1308
BccI CCATC 1 cut(s) 725
BceAI ACGGC 6 cut(s) 275, 676, 993, 1195, 1353, 1432
BcgI CGANNNNNNTGC 4 cut(s) 838, 872, 1501, 1535
BciT130I CCWGG 1 cut(s) 229
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 3 cut(s) 622, 1076, 1498
BcoDI GTCTC 3 cut(s) 536, 640, 1539
BfaI CTAG 5 cut(s) 114, 189, 458, 1284, 1445
BfmI CTRYAG 1 cut(s) 249
BfuI GTATCC 1 cut(s) 15
BglI GCCNNNNNGGC 1 cut(s) 660
BglII AGATCT 1 cut(s) 1592
BmcAI AGTACT 2 cut(s) 968, 1430
Bme1390I CCNGG 4 cut(s) 229, 622, 1076, 1498
Bme18I GGWCC 4 cut(s) 445, 874, 922, 1605
BmgBI CACGTC 1 cut(s) 1379
BmgT120I GGNCC 5 cut(s) 445, 874, 922, 1362, 1605
BmiI GGNNCC 3 cut(s) 280, 564, 1364
BmrFI CCNGG 4 cut(s) 229, 622, 1076, 1498
BmsI GCATC 4 cut(s) 214, 803, 1163, 1509
BpiI GAAGAC 2 cut(s) 87, 1533
BplI GAGNNNNNCTC 2 cut(s) 953, 985
Bpu10I CCTNAGC 1 cut(s) 890
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 3 cut(s) 622, 1076, 1498
BsaHI GRCGYC 1 cut(s) 1142
BsaI GGTCTC 2 cut(s) 536, 640
BsaJI CCNNGG 5 cut(s) 213, 228, 262, 621, 1045
BsaWI WCCGGW 3 cut(s) 118, 565, 1092
BsaXI ACNNNNNCTCC 4 cut(s) 453, 483, 1388, 1418
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 228, 871, 1046
Bse118I RCCGGY 3 cut(s) 118, 475, 1401
Bse1I ACTGG 3 cut(s) 8, 1267, 1316
BseBI CCWGG 1 cut(s) 229
BseDI CCNNGG 5 cut(s) 213, 228, 262, 621, 1045
BseGI GGATG 3 cut(s) 672, 740, 1195
BseLI CCNNNNNNNGG 4 cut(s) 14, 228, 871, 1046
BseMII CTCAG 3 cut(s) 86, 705, 881
BseNI ACTGG 3 cut(s) 8, 1267, 1316
BseRI GAGGAG 2 cut(s) 977, 1574
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 4 cut(s) 918, 943, 999, 1308
Bsh1236I CGCG 4 cut(s) 1047, 1183, 1375, 1471
Bsh1285I CGRYCG 4 cut(s) 306, 570, 621, 1386
BshFI GGCC 6 cut(s) 112, 267, 1079, 1315, 1363, 1423
BshTI ACCGGT 1 cut(s) 118
BsiEI CGRYCG 4 cut(s) 306, 570, 621, 1386
BsiHKAI GWGCWC 1 cut(s) 495
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 4 cut(s) 14, 228, 871, 1046
BsmAI GTCTC 3 cut(s) 536, 640, 1539
BsmBI CGTCTC 1 cut(s) 1539
BsmFI GGGAC 1 cut(s) 638
BsmI GAATGC 2 cut(s) 46, 663
BsnI GGCC 6 cut(s) 112, 267, 1079, 1315, 1363, 1423
Bso31I GGTCTC 2 cut(s) 536, 640
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 495, 1076
Bsp19I CCATGG 1 cut(s) 213
BspANI GGCC 6 cut(s) 112, 267, 1079, 1315, 1363, 1423
BspCNI CTCAG 3 cut(s) 87, 704, 882
BspFNI CGCG 4 cut(s) 1047, 1183, 1375, 1471
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 3 cut(s) 280, 564, 1364
BspPI GGATC 4 cut(s) 27, 596, 1176, 1265
BspT104I TTCGAA 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 536, 640
BsrBI CCGCTC 1 cut(s) 246
BsrFI RCCGGY 3 cut(s) 118, 475, 1401
BsrI ACTGG 3 cut(s) 8, 1267, 1316
BssAI RCCGGY 3 cut(s) 118, 475, 1401
BssECI CCNNGG 5 cut(s) 213, 228, 262, 621, 1045
BssNI GRCGYC 1 cut(s) 1142
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 795
Bst6I CTCTTC 3 cut(s) 131, 550, 634
BstACI GRCGYC 1 cut(s) 1142
BstBI TTCGAA 1 cut(s) 69
BstC8I GCNNGC 9 cut(s) 269, 422, 477, 481, 1152, 1345, 1392, 1421, 1467
BstDEI CTNAG 3 cut(s) 95, 691, 890
BstDSI CCRYGG 3 cut(s) 213, 262, 1045
BstF5I GGATG 3 cut(s) 672, 740, 1195
BstFNI CGCG 4 cut(s) 1047, 1183, 1375, 1471
BstHHI GCGC 5 cut(s) 243, 818, 906, 1375, 1471
BstMAI GTCTC 3 cut(s) 536, 640, 1539
BstMCI CGRYCG 4 cut(s) 306, 570, 621, 1386
BstMWI GCNNNNNNNGC 6 cut(s) 109, 660, 1009, 1321, 1327, 1379
BstNI CCWGG 1 cut(s) 229
BstNSI RCATGY 2 cut(s) 904, 1347
BstSCI CCNGG 4 cut(s) 227, 620, 1074, 1496
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 4 cut(s) 1047, 1183, 1375, 1471
BstV1I GCAGC 4 cut(s) 918, 943, 999, 1308
BstV2I GAAGAC 2 cut(s) 87, 1533
BstX2I RGATCY 2 cut(s) 1168, 1592
BstXI CCANNNNNNTGG 2 cut(s) 214, 275
BstYI RGATCY 2 cut(s) 1168, 1592
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 6 cut(s) 112, 267, 1079, 1315, 1363, 1423
BtgI CCRYGG 3 cut(s) 213, 262, 1045
BtrI CACGTC 1 cut(s) 1379
BtsCI GGATG 3 cut(s) 672, 740, 1195
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 4 cut(s) 428, 791, 987, 1323
Cac8I GCNNGC 9 cut(s) 269, 422, 477, 481, 1152, 1345, 1392, 1421, 1467
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 5 cut(s) 243, 818, 906, 1375, 1471
Cfr10I RCCGGY 3 cut(s) 118, 475, 1401
Cfr13I GGNCC 5 cut(s) 445, 874, 922, 1362, 1605
Cfr42I CCGCGG 1 cut(s) 1048
CpoI CGGWCCG 1 cut(s) 874
CseI GACGC 1 cut(s) 1150
Csp6I GTAC 5 cut(s) 967, 1429, 1453, 1477, 1501
CspAI ACCGGT 1 cut(s) 118
CspI CGGWCCG 1 cut(s) 874
CviQI GTAC 5 cut(s) 967, 1429, 1453, 1477, 1501
DdeI CTNAG 3 cut(s) 95, 691, 890
DrdI GACNNNNNNGTC 2 cut(s) 302, 632
DseDI GACNNNNNNGTC 2 cut(s) 302, 632
EaeI YGGCCR 4 cut(s) 265, 1077, 1313, 1421
Eam1104I CTCTTC 3 cut(s) 131, 550, 634
EarI CTCTTC 3 cut(s) 131, 550, 634
EciI GGCGGA 1 cut(s) 1409
Eco130I CCWWGG 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 1076
Eco31I GGTCTC 2 cut(s) 536, 640
Eco32I GATATC 1 cut(s) 773
Eco47I GGWCC 4 cut(s) 445, 874, 922, 1605
Eco57I CTGAAG 2 cut(s) 452, 947
EcoO109I RGGNCCY 1 cut(s) 1362
EcoRI GAATTC 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 227
EcoRV GATATC 1 cut(s) 773
EcoT14I CCWWGG 1 cut(s) 213
EcoT22I ATGCAT 1 cut(s) 1349
EcoT38I GRGCYC 1 cut(s) 1076
ErhI CCWWGG 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 1539
FalI AAGNNNNNCTT 2 cut(s) 1582, 1614
FaqI GGGAC 1 cut(s) 638
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 2 cut(s) 294, 617
FokI GGATG 3 cut(s) 659, 747, 1202
FriOI GRGCYC 1 cut(s) 1076
FspBI CTAG 5 cut(s) 114, 189, 458, 1284, 1445
FspI TGCGCA 1 cut(s) 905
GlaI GCGC 5 cut(s) 242, 817, 905, 1374, 1470
HaeIII GGCC 6 cut(s) 112, 267, 1079, 1315, 1363, 1423
HgaI GACGC 1 cut(s) 1150
HhaI GCGC 5 cut(s) 243, 818, 906, 1375, 1471
Hin1I GRCGYC 1 cut(s) 1142
Hin6I GCGC 5 cut(s) 241, 816, 904, 1373, 1469
HinP1I GCGC 5 cut(s) 241, 816, 904, 1373, 1469
HincII GTYRAC 3 cut(s) 295, 618, 1335
HindII GTYRAC 3 cut(s) 295, 618, 1335
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 6 cut(s) 184, 572, 579, 626, 1007, 1178
Hpy166II GTNNAC 9 cut(s) 256, 295, 445, 493, 618, 766, 862, 985, 1335
Hpy188I TCNGA 7 cut(s) 77, 96, 223, 554, 640, 694, 1202
Hpy188III TCNNGA 3 cut(s) 53, 576, 1244
Hpy8I GTNNAC 9 cut(s) 256, 295, 445, 493, 618, 766, 862, 985, 1335
Hpy99I CGWCG 3 cut(s) 296, 1144, 1380
HpyAV CCTTC 3 cut(s) 458, 689, 718
HpyCH4III ACNGT 1 cut(s) 795
HpyCH4IV ACGT 2 cut(s) 996, 1378
HpyCH4V TGCA 6 cut(s) 356, 424, 493, 1116, 1232, 1347
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 660, 1009, 1321, 1327, 1379
HpyF3I CTNAG 3 cut(s) 95, 691, 890
HpySE526I ACGT 2 cut(s) 996, 1378
Hsp92I GRCGYC 1 cut(s) 1142
HspAI GCGC 5 cut(s) 241, 816, 904, 1373, 1469
KroI GCCGGC 1 cut(s) 475
KroNI GCCGGC 1 cut(s) 477
Ksp22I TGATCA 1 cut(s) 322
KspI CCGCGG 1 cut(s) 1048
LmnI GCTCC 2 cut(s) 568, 1147
Lsp1109I GCAGC 4 cut(s) 918, 943, 999, 1308
LweI GCATC 4 cut(s) 214, 803, 1163, 1509
MaeI CTAG 5 cut(s) 114, 189, 458, 1284, 1445
MaeII ACGT 2 cut(s) 996, 1378
MaeIII GTNAC 3 cut(s) 1052, 1258, 1292
MbiI CCGCTC 1 cut(s) 246
MfeI CAATTG 2 cut(s) 1018, 1111
MflI RGATCY 2 cut(s) 1168, 1592
MhlI GDGCHC 2 cut(s) 495, 1076
MlsI TGGCCA 2 cut(s) 267, 1315
MluCI AATT 6 cut(s) 437, 449, 783, 854, 1018, 1111
MluNI TGGCCA 2 cut(s) 267, 1315
MlyI GAGTC 3 cut(s) 193, 620, 1187
MmeI TCCRAC 2 cut(s) 256, 1180
Mox20I TGGCCA 2 cut(s) 267, 1315
Mph1103I ATGCAT 1 cut(s) 1349
MroNI GCCGGC 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 989
MscI TGGCCA 2 cut(s) 267, 1315
MseI TTAA 3 cut(s) 12, 315, 1612
MslI CAYNNNNRTG 2 cut(s) 261, 811
Msp20I TGGCCA 2 cut(s) 267, 1315
MspA1I CMGCKG 1 cut(s) 1047
MspR9I CCNGG 4 cut(s) 229, 622, 1076, 1498
MunI CAATTG 2 cut(s) 1018, 1111
Mva1269I GAATGC 2 cut(s) 46, 663
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 4 cut(s) 1047, 1183, 1375, 1471
MwoI GCNNNNNNNGC 6 cut(s) 109, 660, 1009, 1321, 1327, 1379
NaeI GCCGGC 1 cut(s) 477
NciI CCSGG 3 cut(s) 622, 1076, 1498
NcoI CCATGG 1 cut(s) 213
NgoMIV GCCGGC 1 cut(s) 475
NlaIV GGNNCC 3 cut(s) 280, 564, 1364
NmeAIII GCCGAG 1 cut(s) 1065
NsbI TGCGCA 1 cut(s) 905
NsiI ATGCAT 1 cut(s) 1349
NspI RCATGY 2 cut(s) 904, 1347
NspV TTCGAA 1 cut(s) 69
OliI CACNNNNGTG 1 cut(s) 261
PaeI GCATGC 1 cut(s) 1347
PagI TCATGA 1 cut(s) 1243
PceI AGGCCT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 1136
PctI GAATGC 2 cut(s) 46, 663
PdiI GCCGGC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 3 cut(s) 572, 579, 1007
PflFI GACNNNGTC 1 cut(s) 1486
PflMI CCANNNNNTGG 1 cut(s) 14
PinAI ACCGGT 1 cut(s) 118
Ple19I CGATCG 1 cut(s) 1386
PleI GAGTC 3 cut(s) 192, 620, 1186
PpsI GAGTC 3 cut(s) 192, 620, 1186
Psp6I CCWGG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 3 cut(s) 280, 564, 1364
PspPI GGNCC 5 cut(s) 445, 874, 922, 1362, 1605
PsuI RGATCY 2 cut(s) 1168, 1592
PsyI GACNNNGTC 1 cut(s) 1486
PvuI CGATCG 1 cut(s) 1386
RsaI GTAC 5 cut(s) 968, 1430, 1454, 1478, 1502
RsaNI GTAC 5 cut(s) 967, 1429, 1453, 1477, 1501
RseI CAYNNNNRTG 2 cut(s) 261, 811
Rsr2I CGGWCCG 1 cut(s) 874
RsrII CGGWCCG 1 cut(s) 874
SacII CCGCGG 1 cut(s) 1048
SalI GTCGAC 2 cut(s) 293, 616
SaqAI TTAA 3 cut(s) 12, 315, 1612
Sau96I GGNCC 5 cut(s) 445, 874, 922, 1362, 1605
ScaI AGTACT 2 cut(s) 968, 1430
SchI GAGTC 3 cut(s) 193, 620, 1187
ScrFI CCNGG 4 cut(s) 229, 622, 1076, 1498
SduI GDGCHC 2 cut(s) 495, 1076
SfaNI GCATC 4 cut(s) 214, 803, 1163, 1509
SfcI CTRYAG 1 cut(s) 249
Sfr303I CCGCGG 1 cut(s) 1048
SfuI TTCGAA 1 cut(s) 69
SgrBI CCGCGG 1 cut(s) 1048
SinI GGWCC 4 cut(s) 445, 874, 922, 1605
SmiMI CAYNNNNRTG 2 cut(s) 261, 811
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
SphI GCATGC 1 cut(s) 1347
Sse9I AATT 6 cut(s) 437, 449, 783, 854, 1018, 1111
SseBI AGGCCT 1 cut(s) 112
SspMI CTAG 5 cut(s) 114, 189, 458, 1284, 1445
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 4 cut(s) 227, 620, 1074, 1496
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 795
TaiI ACGT 2 cut(s) 999, 1381
TaqII GACCGA 2 cut(s) 292, 622
TasI AATT 6 cut(s) 437, 449, 783, 854, 1018, 1111
TatI WGTACW 3 cut(s) 966, 1428, 1452
TauI GCSGC 7 cut(s) 61, 246, 290, 849, 1047, 1050, 1343
TfiI GAWTC 3 cut(s) 572, 579, 1007
Tru1I TTAA 3 cut(s) 12, 315, 1612
Tru9I TTAA 3 cut(s) 12, 315, 1612
TscAI CASTG 4 cut(s) 435, 798, 987, 1323
TseI GCWGC 4 cut(s) 906, 931, 1012, 1321
TspDTI ATGAA 7 cut(s) 165, 386, 790, 1024, 1048, 1589, 1590
TspGWI ACGGA 4 cut(s) 251, 783, 887, 974
TspRI CASTG 4 cut(s) 435, 798, 987, 1323
Tth111I GACNNNGTC 1 cut(s) 1486
Van91I CCANNNNNTGG 1 cut(s) 14
VneI GTGCAC 1 cut(s) 491
VpaK11BI GGWCC 4 cut(s) 445, 874, 922, 1605
XapI RAATTY 3 cut(s) 437, 783, 854
XceI RCATGY 2 cut(s) 904, 1347
XcmI CCANNNNNNNNNTGG 1 cut(s) 1323
XmiI GTMKAC 2 cut(s) 294, 617
XmnI GAANNNNTTC 1 cut(s) 989
XspI CTAG 5 cut(s) 114, 189, 458, 1284, 1445
ZrmI AGTACT 2 cut(s) 968, 1430
Zsp2I ATGCAT 1 cut(s) 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.