FvH4_2g38470

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017884
Physical Location & Seq
Forward (+)
27835730 .. 27838916
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38470.t1

Sequence Viewer

Length: 819 bp
ATGAAGGGAGGCAAAGGAGCCACCCACGCGGTTGCGACATGGGTCCGGCGACAGCCGCCGAAGATCAAGGCGTTTTTGGCGGTGGTGTCGGGTATGTCCGCCTTGGTGCTCTTACGTATGATCGTCCACGACCACGACAACCTCTTCGTGGCAGCCGAGGCTGTCCACTCCCTCGGAATATCCGTCCTCATCTACAAGCTTATGAAAGAGAAGACCTGTGCCGGGATTTCACTCAAGTCGCAGGAATTGACGGCTCTGTTTCTAGCTGTTAGGCTGTACTGTAGTTTGGTTATGGAATATGACATACATACGATACTGGATTCGGCTACATTGGTGACCACGCTTTGGGTTATTTATATGATTCGGTTTAAGCTCAGGTCTAGCTACATGGATGACAAGGACAACTTCGCTATCTACTATGTGGTGATTCCTTGTGCTCTGCTTTCATTAGCTGTACATCCCACGACCCATCATTTTATTGTAAACCGAATTGGATGGGCATTCTGTGTTTATCTTGAAGCCGTATCAGTACTACCTCAGCTTCGACTCATGCAAAACACTAAGATTGTTGAACCATTTACGGCACATTATGTTTTTGCCCTGGGAGTCGCACGGTTCTTGAGTTGCGCACATTGGGTCCTCCAGGTAATGGATACTCGAGGACAGTTACTGACAACTTTAGGTTATGGGTTCTGGCCTTGTATGGTTCTTCTCTCAGAAATTGTCCAGACTTTCATACTTGCAGATTTTTGCTACTACTACGTCAAAAGTGCTGTTGATGGGCAACTTGTTCTACGCCTACCCTCTGGAGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

273

Amino Acids

30.59

Weight (kDa)

8.91

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 72 - 214 2.7e-44 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75760 AT1G75760 AT1G75760
fragaria_vesca FvH4_2g38470
rosa_chinensis RchiOBHm_Chr6g0302831
rosa_laevigata RLG00000011134
rosa_multiflora Rmu_ssc0000460.1_g000007
rosa_rugosa Rorug06G0317600
rosa_samantha Rh6AG430800 Rh6BG471900
rosa_wichuraiana Rw6G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 628
AccB7I CCANNNNNTGG 2 cut(s) 345, 649
AccII CGCG 1 cut(s) 29
AciI CCGC 4 cut(s) 29, 56, 80, 99
AfaI GTAC 3 cut(s) 278, 456, 531
AfiI CCNNNNNNNGG 4 cut(s) 148, 222, 345, 649
AgsI TTSAA 2 cut(s) 518, 572
AjnI CCWGG 2 cut(s) 600, 642
AluBI AGCT 6 cut(s) 199, 266, 373, 384, 452, 541
AluI AGCT 6 cut(s) 199, 266, 373, 384, 452, 541
Alw21I GWGCWC 2 cut(s) 111, 439
AlwNI CAGNNNCTG 1 cut(s) 670
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 1 cut(s) 152
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 2 cut(s) 43, 637
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 346, 436
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 2 cut(s) 43, 637
BbsI GAAGAC 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 111, 439
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 164
BccI CCATC 3 cut(s) 477, 489, 773
BceAI ACGGC 3 cut(s) 267, 506, 597
BciT130I CCWGG 2 cut(s) 602, 644
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 2 cut(s) 263, 381
BfmI CTRYAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 2 cut(s) 56, 153
BlsI GCNGC 2 cut(s) 57, 154
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 223, 602, 644
Bme18I GGWCC 2 cut(s) 43, 637
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 2 cut(s) 43, 637
BmiI GGNNCC 3 cut(s) 19, 44, 638
BmrFI CCNGG 3 cut(s) 223, 602, 644
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 374, 537
BpuEI CTTGAG 2 cut(s) 218, 640
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 116
BsaJI CCNNGG 5 cut(s) 102, 156, 172, 600, 601
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 222, 345, 649
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 602, 644
BseDI CCNNGG 5 cut(s) 102, 156, 172, 600, 601
BseGI GGATG 3 cut(s) 397, 457, 500
BseLI CCNNNNNNNGG 4 cut(s) 148, 222, 345, 649
BseMII CTCAG 3 cut(s) 388, 551, 729
BseNI ACTGG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 697
BsiHKAI GWGCWC 2 cut(s) 111, 439
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 2 cut(s) 46, 222
BslI CCNNNNNNNGG 4 cut(s) 148, 222, 345, 649
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 1 cut(s) 697
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 111, 439
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 63, 120
BspACI CCGC 4 cut(s) 29, 56, 80, 99
BspANI GGCC 1 cut(s) 697
BspCNI CTCAG 3 cut(s) 387, 550, 728
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 3 cut(s) 19, 44, 638
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 5 cut(s) 102, 156, 172, 600, 601
BssMI GATC 2 cut(s) 63, 120
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 602, 644
Bst4CI ACNGT 3 cut(s) 281, 615, 666
Bst6I CTCTTC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 116
BstDEI CTNAG 4 cut(s) 374, 537, 561, 715
BstEII GGTNACC 1 cut(s) 334
BstF5I GGATG 3 cut(s) 397, 457, 500
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 2 cut(s) 66, 123
BstMBI GATC 2 cut(s) 63, 120
BstMWI GCNNNNNNNGC 4 cut(s) 26, 55, 77, 158
BstNI CCWGG 2 cut(s) 602, 644
BstPAI GACNNNNGTC 1 cut(s) 41
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 221, 600, 642
BstSFI CTRYAG 1 cut(s) 280
BstSNI TACGTA 1 cut(s) 116
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 1 cut(s) 218
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 1 cut(s) 697
BtsCI GGATG 3 cut(s) 397, 457, 500
CaiI CAGNNNCTG 1 cut(s) 670
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 2 cut(s) 43, 637
Csp6I GTAC 3 cut(s) 277, 455, 530
CviAII CATG 3 cut(s) 39, 388, 550
CviQI GTAC 3 cut(s) 277, 455, 530
DdeI CTNAG 4 cut(s) 374, 537, 561, 715
DpnI GATC 2 cut(s) 65, 122
DpnII GATC 2 cut(s) 63, 120
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 43, 637
Eco88I CYCGRG 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 637
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 600, 642
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 3 cut(s) 42, 391, 553
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 3 cut(s) 38, 387, 549
Fnu4HI GCNGC 2 cut(s) 56, 153
FokI GGATG 3 cut(s) 404, 444, 507
Fsp4HI GCNGC 2 cut(s) 56, 153
FspBI CTAG 2 cut(s) 263, 381
FspI TGCGCA 1 cut(s) 628
GlaI GCGC 1 cut(s) 628
GluI GCNGC 2 cut(s) 56, 153
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 1 cut(s) 697
HapII CCGG 2 cut(s) 46, 222
HhaI GCGC 1 cut(s) 629
Hin1II CATG 3 cut(s) 42, 391, 553
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 5 cut(s) 320, 361, 427, 546, 606
HpaII CCGG 2 cut(s) 46, 222
HphI GGTGA 2 cut(s) 346, 436
Hpy166II GTNNAC 3 cut(s) 127, 166, 484
Hpy188I TCNGA 2 cut(s) 176, 718
Hpy188III TCNNGA 4 cut(s) 515, 619, 727, 807
Hpy8I GTNNAC 3 cut(s) 127, 166, 484
HpyCH4III ACNGT 3 cut(s) 281, 615, 666
HpyCH4IV ACGT 2 cut(s) 115, 762
HpyCH4V TGCA 2 cut(s) 553, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 55, 77, 158
HpyF3I CTNAG 4 cut(s) 374, 537, 561, 715
HpySE526I ACGT 2 cut(s) 115, 762
Hsp92II CATG 3 cut(s) 42, 391, 553
HspAI GCGC 1 cut(s) 627
Kzo9I GATC 2 cut(s) 63, 120
LmnI GCTCC 1 cut(s) 17
Lsp1109I GCAGC 1 cut(s) 164
MaeI CTAG 2 cut(s) 263, 381
MaeII ACGT 2 cut(s) 115, 762
MaeIII GTNAC 2 cut(s) 334, 666
MalI GATC 2 cut(s) 65, 122
MboI GATC 2 cut(s) 63, 120
MboII GAAGA 4 cut(s) 73, 136, 223, 701
MhlI GDGCHC 2 cut(s) 111, 439
MluCI AATT 3 cut(s) 245, 489, 720
MlyI GAGTC 2 cut(s) 540, 615
MnlI CCTC 8 cut(s) 151, 152, 182, 197, 546, 650, 653, 814
MseI TTAA 1 cut(s) 369
MspI CCGG 2 cut(s) 46, 222
MspR9I CCNGG 3 cut(s) 223, 602, 644
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 2 cut(s) 602, 644
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 26, 55, 77, 158
NciI CCSGG 1 cut(s) 223
NdeII GATC 2 cut(s) 63, 120
NlaIII CATG 3 cut(s) 42, 391, 553
NlaIV GGNNCC 3 cut(s) 19, 44, 638
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 334
NsbI TGCGCA 1 cut(s) 628
PaeR7I CTCGAG 1 cut(s) 657
PasI CCCWGGG 1 cut(s) 601
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 180
PctI GAATGC 1 cut(s) 500
PfeI GAWTC 3 cut(s) 320, 361, 427
PflMI CCANNNNNTGG 2 cut(s) 345, 649
PkrI GCNGC 2 cut(s) 57, 154
PleI GAGTC 2 cut(s) 540, 614
PpsI GAGTC 2 cut(s) 540, 614
Ppu21I YACGTR 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 637
PshAI GACNNNNGTC 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 2 cut(s) 600, 642
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 2 cut(s) 600, 642
PspN4I GGNNCC 3 cut(s) 19, 44, 638
PspPI GGNCC 2 cut(s) 43, 637
PspPPI RGGWCCY 1 cut(s) 637
PspXI VCTCGAGB 1 cut(s) 657
PstNI CAGNNNCTG 1 cut(s) 670
RsaI GTAC 3 cut(s) 278, 456, 531
RsaNI GTAC 3 cut(s) 277, 455, 530
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 56, 153
Sau3AI GATC 2 cut(s) 63, 120
Sau96I GGNCC 2 cut(s) 43, 637
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 2 cut(s) 540, 615
ScrFI CCNGG 3 cut(s) 223, 602, 644
SduI GDGCHC 2 cut(s) 111, 439
SfcI CTRYAG 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 43, 637
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 3 cut(s) 233, 619, 657
SmoI CTYRAG 3 cut(s) 233, 619, 657
SnaBI TACGTA 1 cut(s) 116
Sse9I AATT 3 cut(s) 245, 489, 720
SsiI CCGC 4 cut(s) 29, 56, 80, 99
SspMI CTAG 2 cut(s) 263, 381
StyD4I CCNGG 3 cut(s) 221, 600, 642
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 3 cut(s) 281, 615, 666
TaiI ACGT 2 cut(s) 118, 765
TaqI TCGA 2 cut(s) 544, 658
TasI AATT 3 cut(s) 245, 489, 720
TatI WGTACW 3 cut(s) 276, 454, 529
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 3 cut(s) 320, 361, 427
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 4 cut(s) 17, 218, 435, 724
TspGWI ACGGA 1 cut(s) 172
Van91I CCANNNNNTGG 2 cut(s) 345, 649
VpaK11BI GGWCC 2 cut(s) 43, 637
XhoI CTCGAG 1 cut(s) 657
XspI CTAG 2 cut(s) 263, 381
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.