Rmu_ssc0000460.1_g000007

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000460.1
OG0017884
Physical Location & Seq
Reverse (-)
39232 .. 41770
2539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000460.1_g000007.1.cds

Sequence Viewer

Length: 819 bp
atgaagggaggcaaagggcccacccacgcggtggcgacatgggtcaggcgacagccgccgaagatcaaggcttttttggcggtggtgtcgggaatggccgccttggtggtcctccgaatgatcgtgcacgaccacgacaacctcttcgtggcggcggaggctgtccactccctcggaatctccgtcctcatctacaagctcatgaaagagaagacttgcgccgggatttcactcaagtcgcaggaattgacggctctgtttctagctgtgaggctgtattgtagtttggtcatggaatatgacatacacacgatactggattcggctacattggtgaccacgctttgggttatttatatgattcggtttaagctcaggtctagctacatggatgacaaggacaacttcgcaatctactatgtggtgattccgtgtgctctgctttcattagctgtacatcccacgacacatcattttattgtaaaccgaattggctgggcattctgtgtttatcttgaagctgtatcagtactacctcagcttcgagtcatgcaaaacactaagatcgttgaaccctttacggcacattatgtttttgccctgggagttgcacggttcttgagttgcgcacattgggtcctccaggtattggattctcgaggacagttactgacaactttaggttatgggttgtggcctggtatggttcttctctcagaaattgtccagaccttcatacttgctgatttttgctactactatgtcaaaagtgctgttgatgggcaacttgttctacgcttaccctctggggttgtctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

272

Amino Acids

30.46

Weight (kDa)

8.97

Isoelectric Point (pI)

29.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75760 AT1G75760 AT1G75760
fragaria_vesca FvH4_2g38470
rosa_chinensis RchiOBHm_Chr6g0302831
rosa_laevigata RLG00000011134
rosa_multiflora Rmu_ssc0000460.1_g000007
rosa_rugosa Rorug06G0317600
rosa_samantha Rh6AG430800 Rh6BG471900
rosa_wichuraiana Rw6G037320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 628
AccB7I CCANNNNNTGG 3 cut(s) 31, 345, 649
AccII CGCG 1 cut(s) 29
AciI CCGC 6 cut(s) 29, 56, 80, 99, 152, 155
AcoI YGGCCR 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 2 cut(s) 456, 531
AfiI CCNNNNNNNGG 4 cut(s) 31, 148, 345, 649
AgsI TTSAA 2 cut(s) 518, 572
AjnI CCWGG 3 cut(s) 600, 642, 697
AluBI AGCT 7 cut(s) 199, 266, 373, 384, 452, 521, 541
AluI AGCT 7 cut(s) 199, 266, 373, 384, 452, 521, 541
Alw21I GWGCWC 2 cut(s) 129, 439
Alw44I GTGCAC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 670
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 3 cut(s) 17, 96, 695
ApaI GGGCCC 1 cut(s) 21
ApaLI GTGCAC 1 cut(s) 125
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 2 cut(s) 221, 629
AspS9I GGNCC 4 cut(s) 17, 18, 109, 637
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 346, 436
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 2 cut(s) 109, 637
BaeGI GKGCMC 2 cut(s) 21, 129
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 129, 439
BbvCI CCTCAGC 1 cut(s) 537
BccI CCATC 1 cut(s) 773
BceAI ACGGC 2 cut(s) 267, 597
BciT130I CCWGG 3 cut(s) 602, 644, 699
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 2 cut(s) 263, 381
BisI GCNGC 3 cut(s) 56, 99, 153
BlsI GCNGC 3 cut(s) 57, 100, 154
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 4 cut(s) 223, 602, 644, 699
Bme18I GGWCC 2 cut(s) 109, 637
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 17, 18, 109, 637
BmiI GGNNCC 2 cut(s) 19, 638
BmrFI CCNGG 4 cut(s) 223, 602, 644, 699
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 374, 537
BpuEI CTTGAG 2 cut(s) 218, 640
BpuMI CCSGG 1 cut(s) 223
BsaJI CCNNGG 4 cut(s) 102, 172, 600, 601
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 148, 345, 649
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 3 cut(s) 602, 644, 699
BseDI CCNNGG 4 cut(s) 102, 172, 600, 601
BseGI GGATG 2 cut(s) 397, 457
BseLI CCNNNNNNNGG 4 cut(s) 31, 148, 345, 649
BseMII CTCAG 3 cut(s) 388, 551, 729
BseNI ACTGG 1 cut(s) 321
BseSI GKGCMC 2 cut(s) 21, 129
BseYI CCCAGC 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 3 cut(s) 19, 98, 697
BsiHKAI GWGCWC 2 cut(s) 129, 439
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 4 cut(s) 31, 148, 345, 649
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 3 cut(s) 19, 98, 697
BsoBI CYCGRG 1 cut(s) 657
Bsp120I GGGCCC 1 cut(s) 17
Bsp1286I GDGCHC 3 cut(s) 21, 129, 439
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 3 cut(s) 63, 120, 564
BspACI CCGC 6 cut(s) 29, 56, 80, 99, 152, 155
BspANI GGCC 3 cut(s) 19, 98, 697
BspCNI CTCAG 3 cut(s) 387, 550, 728
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 19, 638
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 4 cut(s) 102, 172, 600, 601
BssMI GATC 3 cut(s) 63, 120, 564
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 602, 644, 699
Bst4CI ACNGT 2 cut(s) 615, 666
Bst6I CTCTTC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 454
BstDEI CTNAG 4 cut(s) 374, 537, 561, 715
BstEII GGTNACC 1 cut(s) 334
BstF5I GGATG 2 cut(s) 397, 457
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 2 cut(s) 221, 629
BstKTI GATC 3 cut(s) 66, 123, 567
BstMBI GATC 3 cut(s) 63, 120, 564
BstMWI GCNNNNNNNGC 3 cut(s) 55, 77, 158
BstNI CCWGG 3 cut(s) 602, 644, 699
BstPAI GACNNNNGTC 1 cut(s) 41
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 4 cut(s) 221, 600, 642, 697
BstSLI GKGCMC 2 cut(s) 21, 129
BstUI CGCG 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 19, 98, 697
BtsCI GGATG 2 cut(s) 397, 457
CaiI CAGNNNCTG 1 cut(s) 670
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 221, 629
Cfr13I GGNCC 4 cut(s) 17, 18, 109, 637
Csp6I GTAC 2 cut(s) 455, 530
CviAII CATG 5 cut(s) 39, 202, 292, 388, 550
CviQI GTAC 2 cut(s) 455, 530
DdeI CTNAG 4 cut(s) 374, 537, 561, 715
DpnI GATC 3 cut(s) 65, 122, 566
DpnII GATC 3 cut(s) 63, 120, 564
DraIII CACNNNGTG 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 109, 637
Eco88I CYCGRG 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 17, 637
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 600, 642, 697
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 5 cut(s) 42, 205, 295, 391, 553
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 5 cut(s) 38, 201, 291, 387, 549
Fnu4HI GCNGC 3 cut(s) 56, 99, 153
FokI GGATG 2 cut(s) 404, 444
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 3 cut(s) 56, 99, 153
FspBI CTAG 2 cut(s) 263, 381
FspI TGCGCA 1 cut(s) 628
GlaI GCGC 2 cut(s) 220, 628
GluI GCNGC 3 cut(s) 56, 99, 153
GsaI CCCAGC 1 cut(s) 499
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 3 cut(s) 19, 98, 697
HapII CCGG 1 cut(s) 222
HhaI GCGC 2 cut(s) 221, 629
Hin1II CATG 5 cut(s) 42, 205, 295, 391, 553
Hin6I GCGC 2 cut(s) 219, 627
HinP1I GCGC 2 cut(s) 219, 627
HinfI GANTC 6 cut(s) 177, 320, 361, 427, 546, 653
HpaII CCGG 1 cut(s) 222
HphI GGTGA 2 cut(s) 346, 436
Hpy166II GTNNAC 3 cut(s) 127, 166, 484
Hpy188I TCNGA 3 cut(s) 116, 176, 718
Hpy188III TCNNGA 6 cut(s) 90, 202, 515, 619, 657, 727
Hpy8I GTNNAC 3 cut(s) 127, 166, 484
HpyAV CCTTC 1 cut(s) 742
HpyCH4III ACNGT 2 cut(s) 615, 666
HpyCH4V TGCA 3 cut(s) 127, 553, 611
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 77, 158
HpyF3I CTNAG 4 cut(s) 374, 537, 561, 715
Hsp92II CATG 5 cut(s) 42, 205, 295, 391, 553
HspAI GCGC 2 cut(s) 219, 627
Kzo9I GATC 3 cut(s) 63, 120, 564
MaeI CTAG 2 cut(s) 263, 381
MaeIII GTNAC 2 cut(s) 334, 666
MalI GATC 3 cut(s) 65, 122, 566
MboI GATC 3 cut(s) 63, 120, 564
MboII GAAGA 4 cut(s) 73, 136, 223, 701
MhlI GDGCHC 3 cut(s) 21, 129, 439
MluCI AATT 3 cut(s) 245, 489, 720
MlyI GAGTC 1 cut(s) 555
MseI TTAA 1 cut(s) 369
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 4 cut(s) 223, 602, 644, 699
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 3 cut(s) 602, 644, 699
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 55, 77, 158
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 63, 120, 564
NlaIII CATG 5 cut(s) 42, 205, 295, 391, 553
NlaIV GGNNCC 2 cut(s) 19, 638
NmuCI GTSAC 1 cut(s) 334
NsbI TGCGCA 1 cut(s) 628
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 500
PfeI GAWTC 5 cut(s) 177, 320, 361, 427, 653
PflMI CCANNNNNTGG 3 cut(s) 31, 345, 649
PkrI GCNGC 3 cut(s) 57, 100, 154
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 637
PshAI GACNNNNGTC 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 3 cut(s) 600, 642, 697
PspEI GGTNACC 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 495
PspGI CCWGG 3 cut(s) 600, 642, 697
PspN4I GGNNCC 2 cut(s) 19, 638
PspOMI GGGCCC 1 cut(s) 17
PspPI GGNCC 4 cut(s) 17, 18, 109, 637
PspPPI RGGWCCY 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 670
RsaI GTAC 2 cut(s) 456, 531
RsaNI GTAC 2 cut(s) 455, 530
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 56, 99, 153
Sau3AI GATC 3 cut(s) 63, 120, 564
Sau96I GGNCC 4 cut(s) 17, 18, 109, 637
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 4 cut(s) 223, 602, 644, 699
SduI GDGCHC 3 cut(s) 21, 129, 439
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 109, 637
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 3 cut(s) 233, 619, 657
SmoI CTYRAG 3 cut(s) 233, 619, 657
Sse9I AATT 3 cut(s) 245, 489, 720
SsiI CCGC 6 cut(s) 29, 56, 80, 99, 152, 155
SspMI CTAG 2 cut(s) 263, 381
StyD4I CCNGG 4 cut(s) 221, 600, 642, 697
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 615, 666
TaqI TCGA 2 cut(s) 544, 658
TasI AATT 3 cut(s) 245, 489, 720
TatI WGTACW 2 cut(s) 454, 529
TauI GCSGC 3 cut(s) 58, 101, 155
TfiI GAWTC 5 cut(s) 177, 320, 361, 427, 653
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 4 cut(s) 17, 218, 435, 724
TspGWI ACGGA 2 cut(s) 172, 420
Van91I CCANNNNNTGG 3 cut(s) 31, 345, 649
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 109, 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 657
XspI CTAG 2 cut(s) 263, 381
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.