FvH4_3g00110

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018391
Physical Location & Seq
Reverse (-)
75259 .. 75915
657 bp
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UTR
Exon/CDS
Intron
FvH4_3g00110.t1

Sequence Viewer

Length: 657 bp
ATGAATGAGCCAAGGTCGCTCTCCTTGATTGCTTCACCGGATCAACTTGCTTATAATGCAGCCAGAGGAAAACAATTGAAACAAAATAAGAAAATGGTAAAATCCTATCATCTCGCAACTCTATCCTTGTTTCAATGCTTCTTCTTCTTGTTCGTAGCAATACCATCACCATGTGCTGCTACCAAGCCATTGAAGCCAACTAAGCTTGTAGACGCTGTTTGTCGCAAAACTAATTCGAGTTACTCCTTCTGTGTGGAGTCTCTCTACAGCGACCCTCGGACTCCAACTGCTGACAGCTACGTGCTCGCCTACATCTCCTTCGGGTTAGCGTACCTCAATGCCTCCGCCACCCAACACCATGTTGCTCGCCTGCTTAACAACAAGACACAAGGAGGGCACCAACTACAACAACTTGGAACCTGTCACCGTGATTATAACAAGGCTGTTTCTGCATTGCAACTCGCTTACAATGACCTCAATTCCGAGACTTTCTTTGAGCTTGCAGATTTGGCGGGTGTTGCTTCTCTTGCTGCCGAGGATTGCCAAGCTGCTTTCAATACCACTTTTTCCCCTCTCACCAACATGAATCGTGATCTCAAGGATCTCTGCAAAATCTGTGTAGTTGTCTCTAACCTCTTCACTGGACTTACTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

219

Amino Acids

23.98

Weight (kDa)

8.75

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 207 5.8e-16 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018391)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00110
prunus_persica Prupe.4G001200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000141
rosa_multiflora Rmu_ssc0000243.1_g000016
rosa_roxburghii Rroxscaffold_1G00075950
rosa_rugosa Rorug04G0381900
rosa_samantha Rh5BG002300 Rh5CG001100 Rh5DG001000
rosa_wichuraiana Rw5G000130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 435
AccB1I GGYRCC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 210
AciI CCGC 2 cut(s) 345, 512
AclWI GGATC 2 cut(s) 48, 609
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 1 cut(s) 332
AgsI TTSAA 4 cut(s) 79, 134, 193, 556
AluBI AGCT 4 cut(s) 205, 297, 499, 548
AluI AGCT 4 cut(s) 205, 297, 499, 548
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 264, 479, 631
AlwI GGATC 2 cut(s) 48, 609
ApeKI GCWGC 4 cut(s) 59, 176, 530, 548
AsuHPI GGTGA 4 cut(s) 27, 159, 416, 568
BaeGI GKGCMC 1 cut(s) 399
BanI GGYRCC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 4 cut(s) 71, 163, 517, 535
BccI CCATC 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 264, 479, 631
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 4 cut(s) 60, 177, 531, 549
BlsI GCNGC 4 cut(s) 61, 178, 532, 550
BmiI GGNNCC 2 cut(s) 398, 418
BpuEI CTTGAG 1 cut(s) 581
BsaAI YACGTR 1 cut(s) 301
BsaJI CCNNGG 3 cut(s) 11, 275, 534
BsaWI WCCGGW 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 452
BseDI CCNNGG 3 cut(s) 11, 275, 534
BseMI GCAATG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 646
BseSI GKGCMC 1 cut(s) 399
BseXI GCAGC 4 cut(s) 71, 163, 517, 535
BshNI GGYRCC 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 1 cut(s) 38
BsmAI GTCTC 3 cut(s) 264, 479, 631
Bsp1286I GDGCHC 2 cut(s) 306, 399
Bsp143I GATC 3 cut(s) 40, 592, 601
BspACI CCGC 2 cut(s) 345, 512
BspLI GGNNCC 2 cut(s) 398, 418
BspPI GGATC 2 cut(s) 48, 609
BspT107I GGYRCC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 452
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 3 cut(s) 11, 275, 534
BssMI GATC 3 cut(s) 40, 592, 601
BssT1I CCWWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 4 cut(s) 306, 367, 371, 501
BstDEI CTNAG 1 cut(s) 201
BstKTI GATC 3 cut(s) 43, 595, 604
BstMAI GTCTC 3 cut(s) 264, 479, 631
BstMBI GATC 3 cut(s) 40, 592, 601
BstMWI GCNNNNNNNGC 8 cut(s) 16, 56, 193, 202, 449, 509, 518, 527
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 399
BstV1I GCAGC 4 cut(s) 71, 163, 517, 535
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 4 cut(s) 306, 367, 371, 501
CseI GACGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 331
CviAII CATG 3 cut(s) 171, 359, 583
CviJI RGCY 9 cut(s) 10, 62, 187, 196, 205, 297, 443, 499, 548
CviKI_1 RGCY 9 cut(s) 10, 62, 187, 196, 205, 297, 443, 499, 548
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 1 cut(s) 201
DpnI GATC 3 cut(s) 42, 594, 603
DpnII GATC 3 cut(s) 40, 592, 601
DraIII CACNNNGTG 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
EciI GGCGGA 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 3 cut(s) 174, 362, 586
FaiI YATR 5 cut(s) 54, 172, 360, 435, 584
FatI CATG 3 cut(s) 170, 358, 582
FauI CCCGC 1 cut(s) 505
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 60, 177, 531, 549
Fsp4HI GCNGC 4 cut(s) 60, 177, 531, 549
GluI GCNGC 4 cut(s) 60, 177, 531, 549
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 221
Hin1II CATG 3 cut(s) 174, 362, 586
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 3 cut(s) 257, 280, 586
HpaII CCGG 1 cut(s) 38
HphI GGTGA 4 cut(s) 27, 159, 416, 568
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 279, 484
Hpy188III TCNNGA 1 cut(s) 590
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 2 cut(s) 256, 328
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 5 cut(s) 59, 452, 457, 503, 609
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 56, 193, 202, 449, 509, 518, 527
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 3 cut(s) 174, 362, 586
Kzo9I GATC 3 cut(s) 40, 592, 601
LpnPI CCDG 5 cut(s) 51, 76, 383, 433, 627
Lsp1109I GCAGC 4 cut(s) 71, 163, 517, 535
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 239, 422
MalI GATC 3 cut(s) 42, 594, 603
MboI GATC 3 cut(s) 40, 592, 601
MboII GAAGA 3 cut(s) 133, 136, 628
MfeI CAATTG 1 cut(s) 74
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 2 cut(s) 306, 399
MluCI AATT 3 cut(s) 74, 232, 478
MlyI GAGTC 2 cut(s) 266, 274
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 9 cut(s) 59, 285, 344, 352, 386, 485, 529, 582, 644
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 169, 581
MspI CCGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 74
MwoI GCNNNNNNNGC 8 cut(s) 16, 56, 193, 202, 449, 509, 518, 527
NdeII GATC 3 cut(s) 40, 592, 601
NlaIII CATG 3 cut(s) 174, 362, 586
NlaIV GGNNCC 2 cut(s) 398, 418
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 586
PkrI GCNGC 4 cut(s) 61, 178, 532, 550
PleI GAGTC 2 cut(s) 265, 274
PpsI GAGTC 2 cut(s) 265, 274
Ppu21I YACGTR 1 cut(s) 301
PsiI TTATAA 2 cut(s) 54, 435
PspN4I GGNNCC 2 cut(s) 398, 418
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 2 cut(s) 169, 581
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 4 cut(s) 60, 177, 531, 549
Sau3AI GATC 3 cut(s) 40, 592, 601
SchI GAGTC 2 cut(s) 266, 274
SduI GDGCHC 2 cut(s) 306, 399
SfcI CTRYAG 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 169, 581
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 3 cut(s) 74, 232, 478
SsiI CCGC 2 cut(s) 345, 512
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 236
TasI AATT 3 cut(s) 74, 232, 478
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 4 cut(s) 59, 176, 530, 548
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 17, 599
TspRI CASTG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.