Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0015781

Physical Location & Seq

Genomic Coordinates Forward (+)

1284357 .. 1286597

Gene Structure 2241 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g02560.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCAATCCAACAGTCTCTGCTTGATGCTCCCAAAGCTACTTCTCGCTGCAAATCTCTCTATCTGATTCTCTCCTTAGCTGCTATTATAGGCTCGGTTACACTTCTTTCCATCCAAGTTATGATCAAAACCAGCTCCTCTAATTCTCCTCCAGGAGTCATGATTGCTGATGTTGATCTACTACAAGGGCTCCTAGAGGAATCCACACCACACTTGCAACAAGCCCTTGAGACGGCGAAAGATGCTTATGCTAAGATCAATGACCCTAAAGATCGAGCGGCTCTTGTCGATTGTGTGGAGCTCATGGAAGTCACCATGGACAGGATTCTTGACTCTAGAGTGGCTCTCAAGAACTTGAAGGTGCAAAATACCATGGAAGATGTTCATACATGGCTTAGCAGCGTACTCACTAACCATGTCACATGCTTGGATGGACTAAAAGGACTATCAGCCCGGACTATAATGGAGCCTAGGCTCAATGGCTTGATATCCAAGTCAAAAGCCTTCCTAGCCAAGTTGGTTGAAATTTCTCCATCAAAAGAGAAAGTGATCGGTGATCAGCCACTGGAAATCGGAGATTTCCCAACATGGGTCAAAAGGAGGGACCGGAGGCTAATGTCGAGGGATTTTCAGAAGGGAGTTGAGGCCAATGTTGTGGTGGCAAACGATGGGAGTGGGAAATACAAGACTGTGAAAGAAGCCGTGGCAGCAGCACCAGATAAGAGCACAACCAGGTATGTCATTTATGTGAAAAAGGGAACATACAAGGAAAATGTTGAGGTTGTGAAGAACAAGAAGAACTTGATGATTGTTGGAGATGGCATGGATAAAACAATCATCACTGGTAGCCTCAATGTTGTTGATGGATCAACCACTTTCAATTCTGCCACTCTAGCTGCTGTTGGTGATGGATTTATTGCACAAGACATTTGGTTCCAAAACACTGCTGGCCCGGACAAGCACCAAGCTGTTGCACTTCGTGTCGGAGCCGATCAATCCGTGATAAACCGGTGTCGGATCGACGCTTACCAAGACACTCTCTATGCCCACTCCAATAGGCAGTTCTACCGAGACTGCTACATAACAGGTACCGTAGACTTCATATTTGGAAATGCGGCCGTAGTACTTCAAAATTGTAAGATAGTGGCACGGAAGCCGGCGAGTGGACAAAACAACATGGTCACGGCACAAGGTCGGACGGACCCTAACCAAAACACCGGGACTTCCATTCAAAAATGCCAAGTAATCGCAAGTTCCGACTTAGAGCCGGTAAAAGGGACGGTCCGGTCATACTTGGGCCGTCCCTGGAAGGAATACTCTAGGACTGTTGTGATGCAGTCCACCATTGGTGACCACATTGATCCGACTGGGTGGTCTATATGGAGTGGAGACTTTGCTCTGAAAACACTATACTATGGTGAATATGCAAATAGTGGACCCGGGGCTGGTACCAGCAAGAGGGTGACTTGGGCCGGGTACCATGTTATTAAAACCGCATCAGAGGCCCAAAGTTTCACCGTTGCTGGGCTTATACAAGGTGGGTCATGGTTGAAGTCCACTGGGGTTTCTTTTACCGAAGGACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0031090 GO:0043086 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098588 GO:0098772 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

529

Amino Acids

57.49

Weight (kDa)

8.9

Isoelectric Point (pI)

25.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	71 - 176	6.4e-16	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	217 - 513	4e-150	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11580 AT1G11580
fragaria_vesca	FvH4_3g02560
malus_domestica	MD05G1341800.v1.1
prunus_persica	Prupe.4G025200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0003871
rosa_laevigata	RLG00000031185
rosa_multiflora	Rmu_sc0011733.1_g000006
rosa_roxburghii	Rroxscaffold_1G00072100
rosa_rugosa	Rorug04G0407600
rosa_samantha	Rh5AG035100 Rh5BG033600 Rh5CG037500 Rh5DG033800
rosa_wichuraiana	Rw5G003210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1285, 1372
Acc65I	GGTACC	3 cut(s)	1088, 1448, 1476
AccB1I	GGYRCC	3 cut(s)	1088, 1448, 1476
AccBSI	CCGCTC	1 cut(s)	278
AccI	GTMKAC	1 cut(s)	1095
AciI	CCGC	3 cut(s)	278, 1115, 1494
AclWI	GGATC	3 cut(s)	874, 1025, 1355
AcoI	YGGCCR	1 cut(s)	1116
AcsI	RAATTY	1 cut(s)	525
AdeI	CACNNNGTG	1 cut(s)	980
AfaI	GTAC	5 cut(s)	405, 1090, 1125, 1450, 1478
AfiI	CCNNNNNNNGG	8 cut(s)	232, 321, 589, 1163, 1274, 1445, 1458, 1524
AgeI	ACCGGT	1 cut(s)	1008
AgsI	TTSAA	6 cut(s)	358, 524, 880, 1130, 1232, 1552
AjnI	CCWGG	3 cut(s)	151, 731, 1304
AjuI	GAANNNNNNNTTGG	2 cut(s)	1046, 1078
AluBI	AGCT	6 cut(s)	38, 80, 135, 301, 896, 968
AluI	AGCT	6 cut(s)	38, 80, 135, 301, 896, 968
Alw21I	GWGCWC	2 cut(s)	303, 728
Alw26I	GTCTC	4 cut(s)	21, 224, 1065, 1383
AlwI	GGATC	3 cut(s)	874, 1025, 1355
AlwNI	CAGNNNCTG	2 cut(s)	19, 565
Ama87I	CYCGRG	1 cut(s)	1439
AoxI	GGCC	6 cut(s)	645, 949, 1116, 1297, 1470, 1503
ApeKI	GCWGC	6 cut(s)	48, 80, 399, 707, 710, 896
ApoI	RAATTY	1 cut(s)	525
ArsI	GACNNNNNNTTYG	2 cut(s)	1088, 1120
AsiGI	ACCGGT	1 cut(s)	1008
Asp700I	GAANNNNTTC	1 cut(s)	381
Asp718I	GGTACC	3 cut(s)	1088, 1448, 1476
AspA2I	CCTAGG	1 cut(s)	470
AspS9I	GGNCC	8 cut(s)	604, 950, 1201, 1282, 1297, 1436, 1470, 1504
AsuC2I	CCSGG	6 cut(s)	454, 953, 1219, 1440, 1441, 1474
AsuHPI	GGTGA	7 cut(s)	304, 566, 917, 1361, 1430, 1474, 1507
AvaI	CYCGRG	1 cut(s)	1439
AvaII	GGWCC	4 cut(s)	604, 1201, 1282, 1436
AvrII	CCTAGG	1 cut(s)	470
BanI	GGYRCC	3 cut(s)	1088, 1448, 1476
BanII	GRGCYC	2 cut(s)	192, 303
Bbv12I	GWGCWC	2 cut(s)	303, 728
BbvI	GCAGC	6 cut(s)	35, 67, 411, 719, 722, 883
BccI	CCATC	7 cut(s)	119, 425, 541, 662, 812, 857, 902
BceAI	ACGGC	5 cut(s)	249, 686, 1103, 1200, 1284
BciT130I	CCWGG	3 cut(s)	153, 733, 1306
BclI	TGATCA	2 cut(s)	123, 556
BcnI	CCSGG	6 cut(s)	454, 953, 1219, 1440, 1441, 1474
BcoDI	GTCTC	4 cut(s)	21, 224, 1065, 1383
BfaI	CTAG	6 cut(s)	194, 336, 471, 509, 893, 1320
BisI	GCNGC	8 cut(s)	49, 81, 279, 400, 708, 711, 897, 1116
BlnI	CCTAGG	1 cut(s)	470
BlpI	GCTNAGC	1 cut(s)	395
BlsI	GCNGC	8 cut(s)	50, 82, 280, 401, 709, 712, 898, 1117
BmcAI	AGTACT	1 cut(s)	1125
Bme1390I	CCNGG	9 cut(s)	153, 454, 733, 953, 1219, 1306, 1440, 1441, 1474
Bme18I	GGWCC	4 cut(s)	604, 1201, 1282, 1436
BmeT110I	CYCGRG	1 cut(s)	1439
BmgT120I	GGNCC	8 cut(s)	604, 950, 1201, 1282, 1297, 1436, 1470, 1504
BmrFI	CCNGG	9 cut(s)	153, 454, 733, 953, 1219, 1306, 1440, 1441, 1474
BmrI	ACTGGG	2 cut(s)	1377, 1569
BmsI	GCATC	4 cut(s)	16, 232, 1323, 1505
BmuI	ACTGGG	2 cut(s)	1377, 1569
BplI	GAGNNNNNCTC	2 cut(s)	330, 362
BpmI	CTGGAG	1 cut(s)	135
Bpu10I	CCTNAGC	1 cut(s)	76
Bpu1102I	GCTNAGC	1 cut(s)	395
BpuEI	CTTGAG	2 cut(s)	248, 332
BpuMI	CCSGG	6 cut(s)	454, 953, 1219, 1440, 1441, 1474
BsaBI	GATNNNNATC	1 cut(s)	174
BsaJI	CCNNGG	7 cut(s)	315, 372, 470, 702, 1304, 1439, 1440
BsaWI	WCCGGW	3 cut(s)	606, 1008, 1284
BsaXI	ACNNNNNCTCC	2 cut(s)	174, 204
Bsc4I	CCNNNNNNNGG	8 cut(s)	232, 321, 589, 1163, 1274, 1445, 1458, 1524
Bse118I	RCCGGY	3 cut(s)	1008, 1156, 1267
Bse1I	ACTGG	4 cut(s)	570, 847, 1372, 1564
Bse8I	GATNNNNATC	1 cut(s)	174
BseBI	CCWGG	3 cut(s)	153, 733, 1306
BseDI	CCNNGG	7 cut(s)	315, 372, 470, 702, 1304, 1439, 1440
BseGI	GGATG	2 cut(s)	111, 436
BseJI	GATNNNNATC	1 cut(s)	174
BseLI	CCNNNNNNNGG	8 cut(s)	232, 321, 589, 1163, 1274, 1445, 1458, 1524
BseNI	ACTGG	4 cut(s)	570, 847, 1372, 1564
BseRI	GAGGAG	2 cut(s)	127, 138
BseX3I	CGGCCG	1 cut(s)	1116
BseXI	GCAGC	6 cut(s)	35, 67, 411, 719, 722, 883
BseYI	CCCAGC	1 cut(s)	1523
Bsh1285I	CGRYCG	1 cut(s)	1119
BshFI	GGCC	6 cut(s)	647, 951, 1118, 1299, 1472, 1505
BshNI	GGYRCC	3 cut(s)	1088, 1448, 1476
BshTI	ACCGGT	1 cut(s)	1008
BsiEI	CGRYCG	1 cut(s)	1119
BsiHKAI	GWGCWC	2 cut(s)	303, 728
BsiHKCI	CYCGRG	1 cut(s)	1439
BslFI	GGGAC	4 cut(s)	617, 1234, 1287, 1291
BslI	CCNNNNNNNGG	8 cut(s)	232, 321, 589, 1163, 1274, 1445, 1458, 1524
BsmAI	GTCTC	4 cut(s)	21, 224, 1065, 1383
BsmBI	CGTCTC	1 cut(s)	224
BsmFI	GGGAC	4 cut(s)	617, 1234, 1287, 1291
BsnI	GGCC	6 cut(s)	647, 951, 1118, 1299, 1472, 1505
BsoBI	CYCGRG	1 cut(s)	1439
Bsp1286I	GDGCHC	3 cut(s)	192, 303, 728
Bsp1720I	GCTNAGC	1 cut(s)	395
Bsp19I	CCATGG	2 cut(s)	315, 372
BspACI	CCGC	3 cut(s)	278, 1115, 1494
BspANI	GGCC	6 cut(s)	647, 951, 1118, 1299, 1472, 1505
BspHI	TCATGA	1 cut(s)	159
BspPI	GGATC	3 cut(s)	874, 1025, 1355
BspT107I	GGYRCC	3 cut(s)	1088, 1448, 1476
BsrBI	CCGCTC	1 cut(s)	278
BsrFI	RCCGGY	3 cut(s)	1008, 1156, 1267
BsrI	ACTGG	4 cut(s)	570, 847, 1372, 1564
BssAI	RCCGGY	3 cut(s)	1008, 1156, 1267
BssECI	CCNNGG	7 cut(s)	315, 372, 470, 702, 1304, 1439, 1440
BssT1I	CCWWGG	3 cut(s)	315, 372, 470
Bst2UI	CCWGG	3 cut(s)	153, 733, 1306
Bst4CI	ACNGT	7 cut(s)	15, 691, 1093, 1282, 1327, 1519, 1584
BstC8I	GCNNGC	2 cut(s)	949, 1158
BstDEI	CTNAG	4 cut(s)	76, 252, 395, 1261
BstDSI	CCRYGG	3 cut(s)	315, 372, 702
BstEII	GGTNACC	1 cut(s)	1349
BstF5I	GGATG	2 cut(s)	111, 436
BstMAI	GTCTC	4 cut(s)	21, 224, 1065, 1383
BstMCI	CGRYCG	1 cut(s)	1119
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 242, 509, 707, 893, 1502
BstNI	CCWGG	3 cut(s)	153, 733, 1306
BstNSI	RCATGY	1 cut(s)	426
BstPI	GGTNACC	1 cut(s)	1349
BstSCI	CCNGG	9 cut(s)	151, 452, 731, 951, 1217, 1304, 1438, 1439, 1472
BstV1I	GCAGC	6 cut(s)	35, 67, 411, 719, 722, 883
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstZI	CGGCCG	1 cut(s)	1116
BsuRI	GGCC	6 cut(s)	647, 951, 1118, 1299, 1472, 1505
BtgI	CCRYGG	3 cut(s)	315, 372, 702
BtsCI	GGATG	2 cut(s)	111, 436
BtsI	GCAGTG	1 cut(s)	942
BtsIMutI	CAGTG	4 cut(s)	563, 840, 942, 1557
Cac8I	GCNNGC	2 cut(s)	949, 1158
CaiI	CAGNNNCTG	2 cut(s)	19, 565
CciI	TCATGA	1 cut(s)	159
Cfr10I	RCCGGY	3 cut(s)	1008, 1156, 1267
Cfr13I	GGNCC	8 cut(s)	604, 950, 1201, 1282, 1297, 1436, 1470, 1504
Cfr9I	CCCGGG	1 cut(s)	1439
CpoI	CGGWCCG	1 cut(s)	1282
CseI	GACGC	1 cut(s)	1031
CsiI	ACCWGGT	1 cut(s)	731
Csp6I	GTAC	5 cut(s)	404, 1089, 1124, 1449, 1477
CspAI	ACCGGT	1 cut(s)	1008
CspI	CGGWCCG	1 cut(s)	1282
CviQI	GTAC	5 cut(s)	404, 1089, 1124, 1449, 1477
DdeI	CTNAG	4 cut(s)	76, 252, 395, 1261
DraIII	CACNNNGTG	1 cut(s)	980
DrdI	GACNNNNNNGTC	2 cut(s)	1285, 1372
DseDI	GACNNNNNNGTC	2 cut(s)	1285, 1372
EaeI	YGGCCR	1 cut(s)	1116
EagI	CGGCCG	1 cut(s)	1116
Ecl136II	GAGCTC	1 cut(s)	301
EclXI	CGGCCG	1 cut(s)	1116
Eco130I	CCWWGG	3 cut(s)	315, 372, 470
Eco24I	GRGCYC	2 cut(s)	192, 303
Eco32I	GATATC	1 cut(s)	489
Eco47I	GGWCC	4 cut(s)	604, 1201, 1282, 1436
Eco52I	CGGCCG	1 cut(s)	1116
Eco53kI	GAGCTC	1 cut(s)	301
Eco88I	CYCGRG	1 cut(s)	1439
Eco91I	GGTNACC	1 cut(s)	1349
EcoICRI	GAGCTC	1 cut(s)	301
EcoO65I	GGTNACC	1 cut(s)	1349
EcoRII	CCWGG	3 cut(s)	151, 731, 1304
EcoRV	GATATC	1 cut(s)	489
EcoT14I	CCWWGG	3 cut(s)	315, 372, 470
EcoT38I	GRGCYC	2 cut(s)	192, 303
ErhI	CCWWGG	3 cut(s)	315, 372, 470
Esp3I	CGTCTC	1 cut(s)	224
FalI	AAGNNNNNCTT	2 cut(s)	785, 817
FaqI	GGGAC	4 cut(s)	617, 1234, 1287, 1291
FbaI	TGATCA	2 cut(s)	123, 556
FblI	GTMKAC	1 cut(s)	1095
Fnu4HI	GCNGC	8 cut(s)	49, 81, 279, 400, 708, 711, 897, 1116
FokI	GGATG	2 cut(s)	98, 443
FriOI	GRGCYC	2 cut(s)	192, 303
Fsp4HI	GCNGC	8 cut(s)	49, 81, 279, 400, 708, 711, 897, 1116
FspBI	CTAG	6 cut(s)	194, 336, 471, 509, 893, 1320
GluI	GCNGC	8 cut(s)	49, 81, 279, 400, 708, 711, 897, 1116
GsaI	CCCAGC	1 cut(s)	1527
GsuI	CTGGAG	1 cut(s)	135
HaeIII	GGCC	6 cut(s)	647, 951, 1118, 1299, 1472, 1505
HgaI	GACGC	1 cut(s)	1031
HinfI	GANTC	5 cut(s)	67, 156, 200, 325, 332
HphI	GGTGA	7 cut(s)	304, 566, 917, 1361, 1430, 1474, 1507
Hpy166II	GTNNAC	5 cut(s)	1096, 1166, 1341, 1436, 1557
Hpy188III	TCNNGA	4 cut(s)	160, 329, 336, 349
Hpy8I	GTNNAC	5 cut(s)	1096, 1166, 1341, 1436, 1557
Hpy99I	CGWCG	1 cut(s)	1025
HpyAV	CCTTC	5 cut(s)	352, 514, 628, 1303, 1571
HpyCH4III	ACNGT	7 cut(s)	15, 691, 1093, 1282, 1327, 1519, 1584
HpyCH4V	TGCA	7 cut(s)	51, 217, 364, 920, 974, 1336, 1427
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 242, 509, 707, 893, 1502
HpyF3I	CTNAG	4 cut(s)	76, 252, 395, 1261
KpnI	GGTACC	3 cut(s)	1092, 1452, 1480
KroI	GCCGGC	1 cut(s)	1156
KroNI	GCCGGC	1 cut(s)	1158
Ksp22I	TGATCA	2 cut(s)	123, 556
LmnI	GCTCC	6 cut(s)	34, 140, 195, 298, 466, 986
Lsp1109I	GCAGC	6 cut(s)	35, 67, 411, 719, 722, 883
LweI	GCATC	4 cut(s)	16, 232, 1323, 1505
MabI	ACCWGGT	1 cut(s)	731
MaeI	CTAG	6 cut(s)	194, 336, 471, 509, 893, 1320
MaeIII	GTNAC	6 cut(s)	97, 310, 418, 1180, 1349, 1462
MbiI	CCGCTC	1 cut(s)	278
MboII	GAAGA	3 cut(s)	389, 799, 808
MhlI	GDGCHC	3 cut(s)	192, 303, 728
MluCI	AATT	4 cut(s)	142, 525, 880, 1132
MlyI	GAGTC	2 cut(s)	165, 326
MmeI	TCCRAC	7 cut(s)	34, 793, 964, 995, 1175, 1281, 1388
MroNI	GCCGGC	1 cut(s)	1156
MroXI	GAANNNNTTC	1 cut(s)	381
MseI	TTAA	1 cut(s)	1488
MslI	CAYNNNNRTG	1 cut(s)	746
MspR9I	CCNGG	9 cut(s)	153, 454, 733, 953, 1219, 1306, 1440, 1441, 1474
MvaI	CCWGG	3 cut(s)	153, 733, 1306
MwoI	GCNNNNNNNGC	6 cut(s)	35, 242, 509, 707, 893, 1502
NaeI	GCCGGC	1 cut(s)	1158
NciI	CCSGG	6 cut(s)	454, 953, 1219, 1440, 1441, 1474
NcoI	CCATGG	2 cut(s)	315, 372
NgoMIV	GCCGGC	1 cut(s)	1156
NmuCI	GTSAC	5 cut(s)	310, 418, 1180, 1349, 1462
NspI	RCATGY	1 cut(s)	426
PagI	TCATGA	1 cut(s)	159
PcsI	WCGNNNNNNNCGW	1 cut(s)	1254
PdiI	GCCGGC	1 cut(s)	1158
PdmI	GAANNNNTTC	1 cut(s)	381
PfeI	GAWTC	3 cut(s)	67, 200, 325
PfoI	TCCNGGA	1 cut(s)	151
PinAI	ACCGGT	1 cut(s)	1008
PkrI	GCNGC	8 cut(s)	50, 82, 280, 401, 709, 712, 898, 1117
PleI	GAGTC	2 cut(s)	164, 326
PpsI	GAGTC	2 cut(s)	164, 326
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	3 cut(s)	151, 731, 1304
PspEI	GGTNACC	1 cut(s)	1349
PspFI	CCCAGC	1 cut(s)	1523
PspGI	CCWGG	3 cut(s)	151, 731, 1304
PspPI	GGNCC	8 cut(s)	604, 950, 1201, 1282, 1297, 1436, 1470, 1504
PstNI	CAGNNNCTG	2 cut(s)	19, 565
RsaI	GTAC	5 cut(s)	405, 1090, 1125, 1450, 1478
RsaNI	GTAC	5 cut(s)	404, 1089, 1124, 1449, 1477
RseI	CAYNNNNRTG	1 cut(s)	746
Rsr2I	CGGWCCG	1 cut(s)	1282
RsrII	CGGWCCG	1 cut(s)	1282
SacI	GAGCTC	1 cut(s)	303
SaqAI	TTAA	1 cut(s)	1488
SatI	GCNGC	8 cut(s)	49, 81, 279, 400, 708, 711, 897, 1116
Sau96I	GGNCC	8 cut(s)	604, 950, 1201, 1282, 1297, 1436, 1470, 1504
ScaI	AGTACT	1 cut(s)	1125
SchI	GAGTC	2 cut(s)	165, 326
ScrFI	CCNGG	9 cut(s)	153, 454, 733, 953, 1219, 1306, 1440, 1441, 1474
SduI	GDGCHC	3 cut(s)	192, 303, 728
SexAI	ACCWGGT	1 cut(s)	731
SfaNI	GCATC	4 cut(s)	16, 232, 1323, 1505
SinI	GGWCC	4 cut(s)	604, 1201, 1282, 1436
SmaI	CCCGGG	1 cut(s)	1441
SmiMI	CAYNNNNRTG	1 cut(s)	746
SmlI	CTYRAG	2 cut(s)	227, 347
SmoI	CTYRAG	2 cut(s)	227, 347
Sse9I	AATT	4 cut(s)	142, 525, 880, 1132
SsiI	CCGC	3 cut(s)	278, 1115, 1494
SspMI	CTAG	6 cut(s)	194, 336, 471, 509, 893, 1320
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	9 cut(s)	151, 452, 731, 951, 1217, 1304, 1438, 1439, 1472
StyI	CCWWGG	3 cut(s)	315, 372, 470
TaaI	ACNGT	7 cut(s)	15, 691, 1093, 1282, 1327, 1519, 1584
TaqI	TCGA	4 cut(s)	274, 288, 620, 1020
TasI	AATT	4 cut(s)	142, 525, 880, 1132
TatI	WGTACW	1 cut(s)	1123
TauI	GCSGC	2 cut(s)	281, 1118
TfiI	GAWTC	3 cut(s)	67, 200, 325
Tru1I	TTAA	1 cut(s)	1488
Tru9I	TTAA	1 cut(s)	1488
TscAI	CASTG	4 cut(s)	570, 847, 949, 1564
TseFI	GTSAC	5 cut(s)	310, 418, 1180, 1349, 1462
TseI	GCWGC	6 cut(s)	48, 80, 399, 707, 710, 896
Tsp45I	GTSAC	5 cut(s)	310, 418, 1180, 1349, 1462
TspDTI	ATGAA	2 cut(s)	374, 1090
TspGWI	ACGGA	3 cut(s)	988, 1165, 1214
TspMI	CCCGGG	1 cut(s)	1439
TspRI	CASTG	4 cut(s)	570, 847, 949, 1564
VpaK11BI	GGWCC	4 cut(s)	604, 1201, 1282, 1436
XapI	RAATTY	1 cut(s)	525
XbaI	TCTAGA	1 cut(s)	335
XceI	RCATGY	1 cut(s)	426
XcmI	CCANNNNNNNNNTGG	2 cut(s)	655, 944
XmaI	CCCGGG	1 cut(s)	1439
XmaJI	CCTAGG	1 cut(s)	470
XmiI	GTMKAC	1 cut(s)	1095
XmnI	GAANNNNTTC	1 cut(s)	381
XspI	CTAG	6 cut(s)	194, 336, 471, 509, 893, 1320
ZrmI	AGTACT	1 cut(s)	1125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.