Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0015781

Physical Location & Seq

Genomic Coordinates Forward (+)

46238045 .. 46240318

Gene Structure 2274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1341800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGCAATCCAGCAGCCTCTCCTTGAAGCTTTCAAAACTACAACTTCCCACTGCAAACCCTACTCTCTAGTTCTCTGCTTAGTTGCTGTTATAAGCTCAACTACTCTTTTTTCCATCCATGTGCTAACCAAATTCGCCTCGTCCTCTAATCCTCCTTTAACTGGCTTAACCCAAGTCTGCGATCACGCGCTTGATCAGAAATCATGCTTATCCATAGTCTCAGAAACATCGCATGGCACAACCAAGCCAATGAGTGGTGCTGATCTATTACAAACCATCCTACAAAATTCCATGCCACAATTACAAAAAACCCTAGATATTGCCAAAGTTGCGCACTCTCGGATCAACAGTCCAAAAGATCGAGCGGCTATCGTTGATTGCGTGGAGCTGATGGATGTGACCAAAGACAGAGTTATGGACTCCATGGTAGCTCTCCAGAAATTTGGTGCACATAATTATTATTCCCTTGAAGATGTGCATGGTTGGCTTAGTAGTGTACTCACTAACCACGTCACTTGTTTGGATGGACTAGAAGGTCCGGCCAGGACTATGATGGAGCCTTGGCTCAATGACTTGGTATCAAGATCAACAGCCTCCCTAGCCATTGTGGTTGCAATTTCCGATCGATCAAAGTCAAACCCGATTAAGGAGCGGCTGGATGGGATTTTTCCAACTTGGGTCACAAGCAAGGACAGAAGGCTTTTGCTGGATTCTAATTTGGCAAAGGAAAGTAATGCTAATGCTGTGGTTGCAAAGGATGGGAGTGGGAACTATAAGACTGTGAAAGAAGCAGTGGCGGCAGCACCAGATAATAGCAAAACCAGGTACACTATTCATGTGAAAAAGGGAACTTACAAGGAAAATGTCGAGGTCGGGAAGAAAAAGAAGAACTTGATGATGACTGGAGATGGCATGCATAACACAATCATCACTGGCAGCCTCAATGTTGTTGATGGAGCAACCACTTTCAACTCCGCAACTCTAGCTGCTGTTGGTGATGGGTTTATAGCGCAAGACATTTGGTTCCAAAACACAGCTGGGGCAGCAAAGCACCAAGCTGTTGCACTCCGGGTTGGGGCAGATCGATCCGTGATAAACCGGTGTCGTATCGACGCCTACCAAGACACTCTCTATGCCCACTCCAACAGGCAATTCTACAGAGATTCCTACATCACAGGGACAGTGGACTTCATATTCGGAAATGCAGCTGTTGTGTTTCAAAACTGTAAAATCGTGGCCAGAAAGCCCATGACCGGACAAAACAACATGGTCACAGCACAAGGACGTATAGACCCAAATCAAAACACTGGGACTTCTATTCAAAAATGCGATGTAGTTGCGAGCTCGGACTTAGAGCCCGTGAAAGGGACGATCCGATCTTACTTGGGTCGTCCATGGAAGGAGTACTCAAGGACTGTTGTGATGCAGTCCAAGATTGGTGACCACATTGACCCAGCTGGGTGGTCTATTTGGTCTGGGGATTTTGCATTGAAGACATTGTATTATGGAGAGTATGCAAATAGTGGGCCTGGTGCTGGTACTAGCAAGAGAGTGAAATGGGCTGGGTACCATGTGATCACAAGCCCAAAAAAGGCCCAACCTTTCACTGTCGCGAACCTTATACAAGGTGGGTCGTGGTTGAAGTCTACAGGGGTGACTTATACCGAAGGGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0031090 GO:0043086 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098588 GO:0098772 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

559

Amino Acids

60.76

Weight (kDa)

9.09

Isoelectric Point (pI)

27.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	57 - 191	2.9e-19	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	247 - 543	4.1e-148	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11580 AT1G11580
fragaria_vesca	FvH4_3g02560
malus_domestica	MD05G1341800.v1.1
prunus_persica	Prupe.4G025200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0003871
rosa_laevigata	RLG00000031185
rosa_multiflora	Rmu_sc0011733.1_g000006
rosa_roxburghii	Rroxscaffold_1G00072100
rosa_rugosa	Rorug04G0407600
rosa_samantha	Rh5AG035100 Rh5BG033600 Rh5CG037500 Rh5DG033800
rosa_wichuraiana	Rw5G003210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	92
AasI	GACNNNNNNGTC	1 cut(s)	534
Acc16I	TGCGCA	1 cut(s)	333
Acc65I	GGTACC	1 cut(s)	1566
AccB1I	GGYRCC	1 cut(s)	1566
AccB7I	CCANNNNNTGG	1 cut(s)	254
AccBSI	CCGCTC	2 cut(s)	365, 652
AccI	GTMKAC	1 cut(s)	1646
AccII	CGCG	2 cut(s)	188, 1613
AciI	CCGC	4 cut(s)	365, 652, 797, 975
AclWI	GGATC	3 cut(s)	350, 1080, 1366
AcoI	YGGCCR	2 cut(s)	540, 1236
AcsI	RAATTY	3 cut(s)	131, 286, 440
AcyI	GRCGYC	1 cut(s)	1113
AfaI	GTAC	5 cut(s)	498, 827, 1406, 1540, 1568
AfiI	CCNNNNNNNGG	9 cut(s)	161, 254, 646, 1075, 1253, 1364, 1365, 1535, 1591
AgeI	ACCGGT	1 cut(s)	1098
AgsI	TTSAA	8 cut(s)	26, 34, 470, 970, 1220, 1322, 1492, 1642
AjiI	CACGTC	1 cut(s)	511
AjnI	CCWGG	3 cut(s)	542, 821, 1528
AjuI	GAANNNNNNNTTGG	2 cut(s)	1136, 1168
Alw21I	GWGCWC	2 cut(s)	451, 1346
Alw26I	GTCTC	1 cut(s)	223
Alw44I	GTGCAC	1 cut(s)	447
AlwI	GGATC	3 cut(s)	350, 1080, 1366
AoxI	GGCC	4 cut(s)	540, 1236, 1526, 1593
ApaLI	GTGCAC	1 cut(s)	447
ApeKI	GCWGC	6 cut(s)	13, 800, 936, 986, 1043, 1205
ApoI	RAATTY	3 cut(s)	131, 286, 440
ArsI	GACNNNNNNTTYG	2 cut(s)	1178, 1210
AsiGI	ACCGGT	1 cut(s)	1098
Asp718I	GGTACC	1 cut(s)	1566
AspLEI	GCGC	3 cut(s)	190, 334, 1012
AspS9I	GGNCC	3 cut(s)	536, 1526, 1594
AsuC2I	CCSGG	1 cut(s)	1070
AsuHPI	GGTGA	3 cut(s)	1007, 1451, 1666
AvaII	GGWCC	1 cut(s)	536
BaeGI	GKGCMC	1 cut(s)	451
BalI	TGGCCA	1 cut(s)	1238
BanI	GGYRCC	1 cut(s)	1566
BanII	GRGCYC	2 cut(s)	1346, 1359
BbsI	GAAGAC	1 cut(s)	1499
Bbv12I	GWGCWC	2 cut(s)	451, 1346
BbvI	GCAGC	6 cut(s)	25, 812, 948, 973, 1055, 1217
BcgI	CGANNNNNNTGC	2 cut(s)	1319, 1353
BciT130I	CCWGG	3 cut(s)	544, 823, 1530
BclI	TGATCA	2 cut(s)	193, 1575
BcnI	CCSGG	1 cut(s)	1070
BcoDI	GTCTC	1 cut(s)	223
BfaI	CTAG	6 cut(s)	68, 314, 530, 599, 983, 1542
BfmI	CTRYAG	2 cut(s)	1156, 1647
BisI	GCNGC	9 cut(s)	14, 366, 653, 798, 801, 937, 987, 1044, 1206
BlsI	GCNGC	9 cut(s)	15, 367, 654, 799, 802, 938, 988, 1045, 1207
BmcAI	AGTACT	1 cut(s)	1406
Bme1390I	CCNGG	4 cut(s)	544, 823, 1070, 1530
Bme18I	GGWCC	1 cut(s)	536
BmgBI	CACGTC	1 cut(s)	511
BmgT120I	GGNCC	3 cut(s)	536, 1526, 1594
BmiI	GGNNCC	3 cut(s)	558, 1025, 1568
BmrFI	CCNGG	4 cut(s)	544, 823, 1070, 1530
BmrI	ACTGGG	1 cut(s)	1317
BmsI	GCATC	1 cut(s)	1413
BmuI	ACTGGG	1 cut(s)	1317
BpiI	GAAGAC	1 cut(s)	1499
BpmI	CTGGAG	2 cut(s)	419, 924
BpuEI	CTTGAG	1 cut(s)	1393
BpuMI	CCSGG	1 cut(s)	1070
Bsa29I	ATCGAT	2 cut(s)	625, 1084
BsaHI	GRCGYC	1 cut(s)	1113
BsaJI	CCNNGG	3 cut(s)	423, 560, 1394
BsaWI	WCCGGW	2 cut(s)	1098, 1253
Bsc4I	CCNNNNNNNGG	9 cut(s)	161, 254, 646, 1075, 1253, 1364, 1365, 1535, 1591
Bse118I	RCCGGY	1 cut(s)	1098
Bse1I	ACTGG	4 cut(s)	166, 907, 937, 1312
BseBI	CCWGG	3 cut(s)	544, 823, 1530
BseCI	ATCGAT	2 cut(s)	625, 1084
BseDI	CCNNGG	3 cut(s)	423, 560, 1394
BseGI	GGATG	6 cut(s)	114, 276, 400, 529, 664, 763
BseLI	CCNNNNNNNGG	9 cut(s)	161, 254, 646, 1075, 1253, 1364, 1365, 1535, 1591
BseMII	CTCAG	1 cut(s)	234
BseNI	ACTGG	4 cut(s)	166, 907, 937, 1312
BseSI	GKGCMC	1 cut(s)	451
BseXI	GCAGC	6 cut(s)	25, 812, 948, 973, 1055, 1217
BseYI	CCCAGC	4 cut(s)	1037, 1453, 1457, 1562
Bsh1236I	CGCG	2 cut(s)	188, 1613
Bsh1285I	CGRYCG	1 cut(s)	625
BshFI	GGCC	4 cut(s)	542, 1238, 1528, 1595
BshNI	GGYRCC	1 cut(s)	1566
BshTI	ACCGGT	1 cut(s)	1098
BshVI	ATCGAT	2 cut(s)	625, 1084
BsiEI	CGRYCG	1 cut(s)	625
BsiHKAI	GWGCWC	2 cut(s)	451, 1346
BsiSI	CCGG	4 cut(s)	539, 1069, 1099, 1254
BslFI	GGGAC	3 cut(s)	1192, 1324, 1381
BslI	CCNNNNNNNGG	9 cut(s)	161, 254, 646, 1075, 1253, 1364, 1365, 1535, 1591
BsmAI	GTCTC	1 cut(s)	223
BsmFI	GGGAC	3 cut(s)	1192, 1324, 1381
BsnI	GGCC	4 cut(s)	542, 1238, 1528, 1595
Bsp1286I	GDGCHC	3 cut(s)	451, 1346, 1359
Bsp19I	CCATGG	2 cut(s)	423, 1394
Bsp68I	TCGCGA	1 cut(s)	1613
BspACI	CCGC	4 cut(s)	365, 652, 797, 975
BspANI	GGCC	4 cut(s)	542, 1238, 1528, 1595
BspCNI	CTCAG	1 cut(s)	233
BspDI	ATCGAT	2 cut(s)	625, 1084
BspFNI	CGCG	2 cut(s)	188, 1613
BspLI	GGNNCC	3 cut(s)	558, 1025, 1568
BspPI	GGATC	3 cut(s)	350, 1080, 1366
BspT107I	GGYRCC	1 cut(s)	1566
BsrBI	CCGCTC	2 cut(s)	365, 652
BsrFI	RCCGGY	1 cut(s)	1098
BsrI	ACTGG	4 cut(s)	166, 907, 937, 1312
BssAI	RCCGGY	1 cut(s)	1098
BssECI	CCNNGG	3 cut(s)	423, 560, 1394
BssNI	GRCGYC	1 cut(s)	1113
BssT1I	CCWWGG	3 cut(s)	423, 560, 1394
Bst2UI	CCWGG	3 cut(s)	544, 823, 1530
Bst4CI	ACNGT	6 cut(s)	350, 781, 1183, 1226, 1417, 1609
BstACI	GRCGYC	1 cut(s)	1113
BstC8I	GCNNGC	2 cut(s)	914, 1342
BstDEI	CTNAG	4 cut(s)	79, 220, 488, 1351
BstDSI	CCRYGG	2 cut(s)	423, 1394
BstEII	GGTNACC	1 cut(s)	1439
BstF5I	GGATG	6 cut(s)	114, 276, 400, 529, 664, 763
BstFNI	CGCG	2 cut(s)	188, 1613
BstHHI	GCGC	3 cut(s)	190, 334, 1012
BstMAI	GTCTC	1 cut(s)	223
BstMCI	CGRYCG	1 cut(s)	625
BstMWI	GCNNNNNNNGC	7 cut(s)	329, 484, 599, 749, 797, 983, 1043
BstNI	CCWGG	3 cut(s)	544, 823, 1530
BstNSI	RCATGY	1 cut(s)	916
BstPI	GGTNACC	1 cut(s)	1439
BstSCI	CCNGG	4 cut(s)	542, 821, 1068, 1528
BstSFI	CTRYAG	2 cut(s)	1156, 1647
BstSLI	GKGCMC	1 cut(s)	451
BstUI	CGCG	2 cut(s)	188, 1613
BstV1I	GCAGC	6 cut(s)	25, 812, 948, 973, 1055, 1217
BstV2I	GAAGAC	1 cut(s)	1499
BstXI	CCANNNNNNTGG	2 cut(s)	443, 1461
Bsu15I	ATCGAT	2 cut(s)	625, 1084
BsuRI	GGCC	4 cut(s)	542, 1238, 1528, 1595
BsuTUI	ATCGAT	2 cut(s)	625, 1084
BtgI	CCRYGG	2 cut(s)	423, 1394
BtgZI	GCGATG	2 cut(s)	213, 1344
BtrI	CACGTC	1 cut(s)	511
BtsCI	GGATG	6 cut(s)	114, 276, 400, 529, 664, 763
BtsI	GCAGTG	2 cut(s)	49, 798
BtsIMutI	CAGTG	6 cut(s)	49, 798, 930, 1188, 1305, 1605
BtuMI	TCGCGA	1 cut(s)	1613
Cac8I	GCNNGC	2 cut(s)	914, 1342
CfoI	GCGC	3 cut(s)	190, 334, 1012
Cfr10I	RCCGGY	1 cut(s)	1098
Cfr13I	GGNCC	3 cut(s)	536, 1526, 1594
ClaI	ATCGAT	2 cut(s)	625, 1084
CseI	GACGC	1 cut(s)	1121
CsiI	ACCWGGT	1 cut(s)	821
Csp6I	GTAC	5 cut(s)	497, 826, 1405, 1539, 1567
CspAI	ACCGGT	1 cut(s)	1098
CspCI	CAANNNNNGTGG	2 cut(s)	497, 532
CviQI	GTAC	5 cut(s)	497, 826, 1405, 1539, 1567
DdeI	CTNAG	4 cut(s)	79, 220, 488, 1351
DrdI	GACNNNNNNGTC	1 cut(s)	534
DseDI	GACNNNNNNGTC	1 cut(s)	534
EaeI	YGGCCR	2 cut(s)	540, 1236
Ecl136II	GAGCTC	1 cut(s)	1344
Eco130I	CCWWGG	3 cut(s)	423, 560, 1394
Eco24I	GRGCYC	2 cut(s)	1346, 1359
Eco47I	GGWCC	1 cut(s)	536
Eco53kI	GAGCTC	1 cut(s)	1344
Eco91I	GGTNACC	1 cut(s)	1439
EcoICRI	GAGCTC	1 cut(s)	1344
EcoO65I	GGTNACC	1 cut(s)	1439
EcoRII	CCWGG	3 cut(s)	542, 821, 1528
EcoT14I	CCWWGG	3 cut(s)	423, 560, 1394
EcoT22I	ATGCAT	1 cut(s)	918
EcoT38I	GRGCYC	2 cut(s)	1346, 1359
ErhI	CCWWGG	3 cut(s)	423, 560, 1394
FalI	AAGNNNNNCTT	2 cut(s)	875, 907
FaqI	GGGAC	3 cut(s)	1192, 1324, 1381
FbaI	TGATCA	2 cut(s)	193, 1575
FblI	GTMKAC	1 cut(s)	1646
Fnu4HI	GCNGC	9 cut(s)	14, 366, 653, 798, 801, 937, 987, 1044, 1206
FokI	GGATG	6 cut(s)	101, 263, 407, 536, 671, 770
FriOI	GRGCYC	2 cut(s)	1346, 1359
Fsp4HI	GCNGC	9 cut(s)	14, 366, 653, 798, 801, 937, 987, 1044, 1206
FspBI	CTAG	6 cut(s)	68, 314, 530, 599, 983, 1542
FspI	TGCGCA	1 cut(s)	333
GlaI	GCGC	3 cut(s)	189, 333, 1011
GluI	GCNGC	9 cut(s)	14, 366, 653, 798, 801, 937, 987, 1044, 1206
GsaI	CCCAGC	4 cut(s)	1041, 1457, 1461, 1566
GsuI	CTGGAG	2 cut(s)	419, 924
HaeIII	GGCC	4 cut(s)	542, 1238, 1528, 1595
HapII	CCGG	4 cut(s)	539, 1069, 1099, 1254
HgaI	GACGC	1 cut(s)	1121
HhaI	GCGC	3 cut(s)	190, 334, 1012
Hin1I	GRCGYC	1 cut(s)	1113
Hin6I	GCGC	3 cut(s)	188, 332, 1010
HinP1I	GCGC	3 cut(s)	188, 332, 1010
HindIII	AAGCTT	1 cut(s)	27
HinfI	GANTC	3 cut(s)	419, 710, 1163
HpaII	CCGG	4 cut(s)	539, 1069, 1099, 1254
HphI	GGTGA	3 cut(s)	1007, 1451, 1666
Hpy166II	GTNNAC	5 cut(s)	449, 497, 828, 1186, 1647
Hpy188I	TCNGA	7 cut(s)	198, 223, 342, 622, 1199, 1348, 1376
Hpy188III	TCNNGA	4 cut(s)	436, 582, 874, 1612
Hpy8I	GTNNAC	5 cut(s)	449, 497, 828, 1186, 1647
Hpy99I	CGWCG	1 cut(s)	1115
HpyAV	CCTTC	4 cut(s)	527, 690, 1393, 1661
HpyCH4III	ACNGT	6 cut(s)	350, 781, 1183, 1226, 1417, 1609
HpyCH4IV	ACGT	2 cut(s)	510, 1285
HpyF10VI	GCNNNNNNNGC	7 cut(s)	329, 484, 599, 749, 797, 983, 1043
HpyF3I	CTNAG	4 cut(s)	79, 220, 488, 1351
HpySE526I	ACGT	2 cut(s)	510, 1285
Hsp92I	GRCGYC	1 cut(s)	1113
HspAI	GCGC	3 cut(s)	188, 332, 1010
KpnI	GGTACC	1 cut(s)	1570
Ksp22I	TGATCA	2 cut(s)	193, 1575
LmnI	GCTCC	4 cut(s)	385, 556, 649, 956
Lsp1109I	GCAGC	6 cut(s)	25, 812, 948, 973, 1055, 1217
LweI	GCATC	1 cut(s)	1413
MabI	ACCWGGT	1 cut(s)	821
MaeI	CTAG	6 cut(s)	68, 314, 530, 599, 983, 1542
MaeII	ACGT	2 cut(s)	510, 1285
MaeIII	GTNAC	6 cut(s)	397, 511, 679, 1270, 1439, 1654
MbiI	CCGCTC	2 cut(s)	365, 652
MboII	GAAGA	4 cut(s)	482, 889, 898, 1504
MhlI	GDGCHC	3 cut(s)	451, 1346, 1359
MlsI	TGGCCA	1 cut(s)	1238
MluCI	AATT	8 cut(s)	131, 286, 299, 440, 454, 615, 715, 1151
MluNI	TGGCCA	1 cut(s)	1238
MlyI	GAGTC	1 cut(s)	413
MmeI	TCCRAC	2 cut(s)	695, 1167
MnlI	CCTC	7 cut(s)	27, 148, 154, 162, 604, 862, 950
Mox20I	TGGCCA	1 cut(s)	1238
Mph1103I	ATGCAT	1 cut(s)	918
MscI	TGGCCA	1 cut(s)	1238
MseI	TTAA	3 cut(s)	158, 167, 645
MslI	CAYNNNNRTG	1 cut(s)	119
Msp20I	TGGCCA	1 cut(s)	1238
MspA1I	CMGCKG	3 cut(s)	1037, 1208, 1457
MspI	CCGG	4 cut(s)	539, 1069, 1099, 1254
MspR9I	CCNGG	4 cut(s)	544, 823, 1070, 1530
MvaI	CCWGG	3 cut(s)	544, 823, 1530
MvnI	CGCG	2 cut(s)	188, 1613
MwoI	GCNNNNNNNGC	7 cut(s)	329, 484, 599, 749, 797, 983, 1043
NciI	CCSGG	1 cut(s)	1070
NcoI	CCATGG	2 cut(s)	423, 1394
NlaIV	GGNNCC	3 cut(s)	558, 1025, 1568
NmuCI	GTSAC	6 cut(s)	397, 511, 679, 1270, 1439, 1654
NruI	TCGCGA	1 cut(s)	1613
NsbI	TGCGCA	1 cut(s)	333
NsiI	ATGCAT	1 cut(s)	918
NspI	RCATGY	1 cut(s)	916
PaeI	GCATGC	1 cut(s)	916
PcsI	WCGNNNNNNNCGW	1 cut(s)	378
PfeI	GAWTC	2 cut(s)	710, 1163
PflMI	CCANNNNNTGG	1 cut(s)	254
PinAI	ACCGGT	1 cut(s)	1098
PkrI	GCNGC	9 cut(s)	15, 367, 654, 799, 802, 938, 988, 1045, 1207
Ple19I	CGATCG	1 cut(s)	625
PleI	GAGTC	1 cut(s)	413
PpsI	GAGTC	1 cut(s)	413
PsiI	TTATAA	1 cut(s)	92
Psp124BI	GAGCTC	1 cut(s)	1346
Psp6I	CCWGG	3 cut(s)	542, 821, 1528
PspEI	GGTNACC	1 cut(s)	1439
PspFI	CCCAGC	4 cut(s)	1037, 1453, 1457, 1562
PspGI	CCWGG	3 cut(s)	542, 821, 1528
PspN4I	GGNNCC	3 cut(s)	558, 1025, 1568
PspPI	GGNCC	3 cut(s)	536, 1526, 1594
PvuI	CGATCG	1 cut(s)	625
PvuII	CAGCTG	3 cut(s)	1037, 1208, 1457
RruI	TCGCGA	1 cut(s)	1613
RsaI	GTAC	5 cut(s)	498, 827, 1406, 1540, 1568
RsaNI	GTAC	5 cut(s)	497, 826, 1405, 1539, 1567
RseI	CAYNNNNRTG	1 cut(s)	119
SacI	GAGCTC	1 cut(s)	1346
SaqAI	TTAA	3 cut(s)	158, 167, 645
SatI	GCNGC	9 cut(s)	14, 366, 653, 798, 801, 937, 987, 1044, 1206
Sau96I	GGNCC	3 cut(s)	536, 1526, 1594
ScaI	AGTACT	1 cut(s)	1406
SchI	GAGTC	1 cut(s)	413
ScrFI	CCNGG	4 cut(s)	544, 823, 1070, 1530
SduI	GDGCHC	3 cut(s)	451, 1346, 1359
SexAI	ACCWGGT	1 cut(s)	821
SfaNI	GCATC	1 cut(s)	1413
SfcI	CTRYAG	2 cut(s)	1156, 1647
SinI	GGWCC	1 cut(s)	536
SmiMI	CAYNNNNRTG	1 cut(s)	119
SmlI	CTYRAG	1 cut(s)	1408
SmoI	CTYRAG	1 cut(s)	1408
SphI	GCATGC	1 cut(s)	916
Sse9I	AATT	8 cut(s)	131, 286, 299, 440, 454, 615, 715, 1151
SsiI	CCGC	4 cut(s)	365, 652, 797, 975
SspMI	CTAG	6 cut(s)	68, 314, 530, 599, 983, 1542
SstI	GAGCTC	1 cut(s)	1346
StyD4I	CCNGG	4 cut(s)	542, 821, 1068, 1528
StyI	CCWWGG	3 cut(s)	423, 560, 1394
TaaI	ACNGT	6 cut(s)	350, 781, 1183, 1226, 1417, 1609
TaiI	ACGT	2 cut(s)	513, 1288
TaqI	TCGA	5 cut(s)	361, 625, 867, 1084, 1110
TasI	AATT	8 cut(s)	131, 286, 299, 440, 454, 615, 715, 1151
TatI	WGTACW	2 cut(s)	496, 1404
TauI	GCSGC	3 cut(s)	368, 655, 800
TfiI	GAWTC	2 cut(s)	710, 1163
Tru1I	TTAA	3 cut(s)	158, 167, 645
Tru9I	TTAA	3 cut(s)	158, 167, 645
TscAI	CASTG	6 cut(s)	56, 798, 937, 1188, 1312, 1612
TseFI	GTSAC	6 cut(s)	397, 511, 679, 1270, 1439, 1654
TseI	GCWGC	6 cut(s)	13, 800, 936, 986, 1043, 1205
Tsp45I	GTSAC	6 cut(s)	397, 511, 679, 1270, 1439, 1654
TspDTI	ATGAA	2 cut(s)	824, 1180
TspGWI	ACGGA	1 cut(s)	1078
TspRI	CASTG	6 cut(s)	56, 798, 937, 1188, 1312, 1612
Van91I	CCANNNNNTGG	1 cut(s)	254
VneI	GTGCAC	1 cut(s)	447
VpaK11BI	GGWCC	1 cut(s)	536
XapI	RAATTY	3 cut(s)	131, 286, 440
XceI	RCATGY	1 cut(s)	916
XcmI	CCANNNNNNNNNTGG	2 cut(s)	550, 1034
XmiI	GTMKAC	1 cut(s)	1646
XspI	CTAG	6 cut(s)	68, 314, 530, 599, 983, 1542
ZrmI	AGTACT	1 cut(s)	1406
Zsp2I	ATGCAT	1 cut(s)	918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.