FvH4_3g05890

Protein of unknown function (DUF561)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016283
Physical Location & Seq
Reverse (-)
3416994 .. 3419047
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05890.t1

Sequence Viewer

Length: 915 bp
ATGCAGGCCATTCCAACTTCATCAACCTCTCACTCCCTTCTGAAACCTATCACAAACCCTCTTCTGGGTCATTCTCTGTCCTCAAAACCATGCTTGTCTCTGAAAAGAAGAGCTTCTTTTACCACCAAAGCAGTTCTGTCAACTACTAAAGAAGCAGTCTTGAAAGATTTTCATGAACGCCGAGCTCTCAAGATTATTTCAGGTTTGCAGAATTTTGACAAAGAAAATGTTGCATCAGTCATTACTGCTGCAGAACAGGGAGGAGCAACTCATGTGGATATAGCTTGTGACCCTGAGTTGGTGAAGCTTGCTATTAGTCTAACTTCTCTTCCGGTTTGTGTTTCATCTGTAGATCCAGTGGCATTTCCAGCTGCTGTTGAAGCAGGAGCGTTGATGGTTGAGATTGGAAACTATGACTCGTTTTATGAGATGGGTGTGATTTTCACTCCAGAGCAGATATTGAATTTGACAAAGGAGACCAAGAGACTTCTTCCATCCGTGGCCTTATCAGTCACTGTACCTCATACACTAAGCCTCCCAGATCAGGTCAAGCTTGCAGAGATGCTAGAGCAAGAAGGTGTAGACATCATCCAGACTGAAGGAGGAAAATGTTCCAACCCCTCCCAATCCGGTGTTCTTGGTTTGATTGAGAAGGCCTCACCAACATTAGCAGCAGCATACTCGATTTCACGAGCTGTCAACATTCCTGTCATGTGTTCATCTGGACTGAGTGCAGTTACAGCACCAATGGCCATAACAGCAGGAGCCGCTGGTGTGGGCGTGGGTTCTGCAGTTAACAAGCTCAATGATGTGGTAGCCATGATTGCAGAAGTCAGGAGCATCTCTGATTCATTAGGAGCATCAACTGGTACCAGTAGGCGCGCTAGCAGTGAAGAGAAAACTTTGAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF561

Protein Analysis

305

Amino Acids

31.67

Weight (kDa)

6.23

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF561 PF04481 53 - 287 8.4e-87 Protein of unknown function (DUF561)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016283)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05890 FvH4_3g05890
malus_domestica MD05G1286900.v1.1
prunus_persica Prupe.4G078000_v2.0.a1
pyrus_communis pycom05g26530
rosa_chinensis RchiOBHm_Chr5g0013551
rosa_laevigata RLG00000032027
rosa_multiflora Rmu_sc0002573.1_g000021 Rmu_sc0003458.1_g000024
rosa_roxburghii Rroxscaffold_1G00063020
rosa_rugosa Rorug05G0010900
rosa_samantha Rh5AG105500 Rh5CG113600 Rh5DG100300
rosa_wichuraiana Rw5G009190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 869
AccB1I GGYRCC 1 cut(s) 869
AccI GTMKAC 1 cut(s) 582
AccII CGCG 1 cut(s) 882
AciI CCGC 1 cut(s) 768
AclWI GGATC 1 cut(s) 347
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 2 cut(s) 211, 463
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 2 cut(s) 519, 871
AfiI CCNNNNNNNGG 4 cut(s) 64, 65, 298, 544
AgsI TTSAA 4 cut(s) 163, 380, 463, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 8 cut(s) 113, 185, 284, 307, 371, 553, 695, 802
AluI AGCT 8 cut(s) 113, 185, 284, 307, 371, 553, 695, 802
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 102, 470, 478
AlwI GGATC 1 cut(s) 347
AlwNI CAGNNNCTG 2 cut(s) 374, 515
AoxI GGCC 4 cut(s) 6, 501, 654, 750
ApeKI GCWGC 4 cut(s) 248, 371, 671, 674
ApoI RAATTY 2 cut(s) 211, 463
Asp700I GAANNNNTTC 2 cut(s) 112, 610
Asp718I GGTACC 1 cut(s) 869
AspLEI GCGC 2 cut(s) 882, 884
AsuHPI GGTGA 2 cut(s) 313, 651
AsuNHI GCTAGC 1 cut(s) 884
BalI TGGCCA 1 cut(s) 752
BanI GGYRCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 187
BauI CACGAG 1 cut(s) 690
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 235, 358, 683, 686
BccI CCATC 3 cut(s) 388, 424, 502
BcgI CGANNNNNNTGC 2 cut(s) 663, 697
BcoDI GTCTC 3 cut(s) 102, 470, 478
BfaI CTAG 2 cut(s) 566, 885
BfmI CTRYAG 3 cut(s) 249, 348, 789
BisI GCNGC 5 cut(s) 249, 372, 672, 675, 768
BlsI GCNGC 5 cut(s) 250, 373, 673, 676, 769
BmiI GGNNCC 2 cut(s) 766, 871
BmsI GCATC 4 cut(s) 242, 552, 849, 869
BmtI GCTAGC 1 cut(s) 888
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 498
BsaWI WCCGGW 2 cut(s) 331, 629
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 65, 298, 544
Bse1I ACTGG 3 cut(s) 356, 871, 873
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 2 cut(s) 494, 588
BseLI CCNNNNNNNGG 4 cut(s) 64, 65, 298, 544
BseMII CTCAG 2 cut(s) 285, 719
BseNI ACTGG 3 cut(s) 356, 871, 873
BsePI GCGCGC 1 cut(s) 880
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 4 cut(s) 235, 358, 683, 686
BsgI GTGCAG 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 8, 503, 656, 752
BshNI GGYRCC 1 cut(s) 869
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 2 cut(s) 332, 630
BslI CCNNNNNNNGG 4 cut(s) 64, 65, 298, 544
BsmAI GTCTC 3 cut(s) 102, 470, 478
BsnI GGCC 4 cut(s) 8, 503, 656, 752
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 352, 541
BspACI CCGC 1 cut(s) 768
BspANI GGCC 4 cut(s) 8, 503, 656, 752
BspCNI CTCAG 2 cut(s) 286, 720
BspFNI CGCG 1 cut(s) 882
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 766, 871
BspMAI CTGCAG 2 cut(s) 253, 793
BspOI GCTAGC 1 cut(s) 888
BspPI GGATC 1 cut(s) 347
BspQI GCTCTTC 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 470
BsrI ACTGG 3 cut(s) 356, 871, 873
BssECI CCNNGG 1 cut(s) 498
BssHII GCGCGC 1 cut(s) 880
BssMI GATC 2 cut(s) 352, 541
BssSI CACGAG 1 cut(s) 690
Bst2BI CACGAG 1 cut(s) 690
Bst4CI ACNGT 2 cut(s) 517, 912
Bst6I CTCTTC 4 cut(s) 66, 103, 333, 888
BstC8I GCNNGC 5 cut(s) 6, 309, 555, 882, 886
BstDEI CTNAG 3 cut(s) 294, 530, 728
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 2 cut(s) 494, 588
BstFNI CGCG 1 cut(s) 882
BstHHI GCGC 2 cut(s) 882, 884
BstKTI GATC 2 cut(s) 355, 544
BstMAI GTCTC 3 cut(s) 102, 470, 478
BstMBI GATC 2 cut(s) 352, 541
BstMWI GCNNNNNNNGC 7 cut(s) 368, 380, 740, 749, 758, 767, 824
BstSFI CTRYAG 3 cut(s) 249, 348, 789
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 4 cut(s) 235, 358, 683, 686
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 4 cut(s) 8, 503, 656, 752
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 2 cut(s) 494, 588
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 3 cut(s) 363, 513, 895
Cac8I GCNNGC 5 cut(s) 6, 309, 555, 882, 886
CaiI CAGNNNCTG 2 cut(s) 374, 515
CciI TCATGA 1 cut(s) 172
CfoI GCGC 2 cut(s) 882, 884
Csp6I GTAC 2 cut(s) 518, 870
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviAII CATG 5 cut(s) 90, 173, 272, 712, 820
CviQI GTAC 2 cut(s) 518, 870
DdeI CTNAG 3 cut(s) 294, 530, 728
DpnI GATC 2 cut(s) 354, 543
DpnII GATC 2 cut(s) 352, 541
EaeI YGGCCR 1 cut(s) 750
Eam1104I CTCTTC 4 cut(s) 66, 103, 333, 888
EarI CTCTTC 4 cut(s) 66, 103, 333, 888
Ecl136II GAGCTC 1 cut(s) 185
Eco147I AGGCCT 1 cut(s) 656
Eco24I GRGCYC 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 618
EcoICRI GAGCTC 1 cut(s) 185
EcoT38I GRGCYC 1 cut(s) 187
FaeI CATG 5 cut(s) 93, 176, 275, 715, 823
FalI AAGNNNNNCTT 4 cut(s) 97, 129, 100, 132
FatI CATG 5 cut(s) 89, 172, 271, 711, 819
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 249, 372, 672, 675, 768
FokI GGATG 2 cut(s) 481, 575
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 5 cut(s) 249, 372, 672, 675, 768
FspBI CTAG 2 cut(s) 566, 885
GlaI GCGC 2 cut(s) 881, 883
GluI GCNGC 5 cut(s) 249, 372, 672, 675, 768
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 4 cut(s) 8, 503, 656, 752
HapII CCGG 2 cut(s) 332, 630
HhaI GCGC 2 cut(s) 882, 884
Hin1II CATG 5 cut(s) 93, 176, 275, 715, 823
Hin6I GCGC 2 cut(s) 880, 882
HinP1I GCGC 2 cut(s) 880, 882
HincII GTYRAC 3 cut(s) 141, 700, 796
HindII GTYRAC 3 cut(s) 141, 700, 796
HindIII AAGCTT 2 cut(s) 305, 551
HinfI GANTC 2 cut(s) 416, 848
HpaI GTTAAC 1 cut(s) 796
HpaII CCGG 2 cut(s) 332, 630
HphI GGTGA 2 cut(s) 313, 651
Hpy166II GTNNAC 4 cut(s) 141, 583, 700, 796
Hpy188I TCNGA 3 cut(s) 42, 102, 847
Hpy188III TCNNGA 8 cut(s) 160, 173, 190, 449, 592, 690, 723, 835
Hpy8I GTNNAC 4 cut(s) 141, 583, 700, 796
HpyAV CCTTC 4 cut(s) 47, 569, 593, 646
HpyCH4III ACNGT 2 cut(s) 517, 912
HpyCH4V TGCA 8 cut(s) 4, 208, 233, 251, 557, 734, 791, 827
HpyF10VI GCNNNNNNNGC 7 cut(s) 368, 380, 740, 749, 758, 767, 824
HpyF3I CTNAG 3 cut(s) 294, 530, 728
Hsp92II CATG 5 cut(s) 93, 176, 275, 715, 823
HspAI GCGC 2 cut(s) 880, 882
KpnI GGTACC 1 cut(s) 873
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 2 cut(s) 352, 541
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 5 cut(s) 263, 386, 764, 837, 857
Lsp1109I GCAGC 4 cut(s) 235, 358, 683, 686
LweI GCATC 4 cut(s) 242, 552, 849, 869
MaeI CTAG 2 cut(s) 566, 885
MaeIII GTNAC 3 cut(s) 287, 511, 736
MalI GATC 2 cut(s) 354, 543
MboI GATC 2 cut(s) 352, 541
MboII GAAGA 5 cut(s) 53, 120, 320, 482, 905
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 187
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 2 cut(s) 211, 463
MluNI TGGCCA 1 cut(s) 752
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 2 cut(s) 38, 639
MnlI CCTC 9 cut(s) 37, 69, 91, 254, 531, 545, 596, 631, 667
Mox20I TGGCCA 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 112, 610
MscI TGGCCA 1 cut(s) 752
MseI TTAA 1 cut(s) 795
Msp20I TGGCCA 1 cut(s) 752
MspA1I CMGCKG 2 cut(s) 371, 770
MspI CCGG 2 cut(s) 332, 630
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 7 cut(s) 368, 380, 740, 749, 758, 767, 824
NdeII GATC 2 cut(s) 352, 541
NheI GCTAGC 1 cut(s) 884
NlaIII CATG 5 cut(s) 93, 176, 275, 715, 823
NlaIV GGNNCC 2 cut(s) 766, 871
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 2 cut(s) 287, 511
PagI TCATGA 1 cut(s) 172
PauI GCGCGC 1 cut(s) 880
PceI AGGCCT 1 cut(s) 656
PciSI GCTCTTC 1 cut(s) 103
PdmI GAANNNNTTC 2 cut(s) 112, 610
PfeI GAWTC 1 cut(s) 848
PkrI GCNGC 5 cut(s) 250, 373, 673, 676, 769
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Psp124BI GAGCTC 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 766, 871
PstI CTGCAG 2 cut(s) 253, 793
PstNI CAGNNNCTG 2 cut(s) 374, 515
PsuI RGATCY 1 cut(s) 352
PteI GCGCGC 1 cut(s) 880
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 519, 871
RsaNI GTAC 2 cut(s) 518, 870
SacI GAGCTC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 5 cut(s) 249, 372, 672, 675, 768
Sau3AI GATC 2 cut(s) 352, 541
SchI GAGTC 1 cut(s) 410
SduI GDGCHC 1 cut(s) 187
SfaNI GCATC 4 cut(s) 242, 552, 849, 869
SfcI CTRYAG 3 cut(s) 249, 348, 789
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 2 cut(s) 211, 463
SseBI AGGCCT 1 cut(s) 656
SsiI CCGC 1 cut(s) 768
SspMI CTAG 2 cut(s) 566, 885
SstI GAGCTC 1 cut(s) 187
StuI AGGCCT 1 cut(s) 656
TaaI ACNGT 2 cut(s) 517, 912
TaqI TCGA 1 cut(s) 683
TasI AATT 2 cut(s) 211, 463
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 3 cut(s) 363, 520, 895
TseFI GTSAC 2 cut(s) 287, 511
TseI GCWGC 4 cut(s) 248, 371, 671, 674
Tsp45I GTSAC 2 cut(s) 287, 511
TspDTI ATGAA 6 cut(s) 9, 161, 189, 333, 708, 840
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 3 cut(s) 363, 520, 895
XapI RAATTY 2 cut(s) 211, 463
XmiI GTMKAC 1 cut(s) 582
XmnI GAANNNNTTC 2 cut(s) 112, 610
XspI CTAG 2 cut(s) 566, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.