Rmu_sc0003458.1_g000024

Protein of unknown function (DUF561)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003458.1
OG0016283
Physical Location & Seq
Forward (+)
124522 .. 126372
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003458.1_g000024.1.cds

Sequence Viewer

Length: 924 bp
atgcaggcctcaattttcattccaacttcatcaacctctcactcccttcatcaacctatcacaaaccctcttctgggtcattcgttgtcttcaaaaccatgcttgcatctgaaaagaagagcctctttcaccaccaaagcacttctgtcaactactaaagaatcagtcttgaaagattttcatgaccgcagagctctcaagattatttcaggtttgcagaattttgacaaagaaaatgttgcatcagtcattactgctgcagaacagggaggagcaactcatgtggatatagcttgtgaccctgagttggtgaagcttgctattggtctaacttctcttccagtttgcgtttcttctgtagatcccgaggcatttccagctgctgttgaagcaggagcactaatggttgagattggaaactatgactcattctacgagatgggtgtgattttcactcctgagcagatattaaatctagcaaaacacaccaagaggattcttccatctgtggccttatcagtcactgtaccacatacactaagccttccagaccaggtcaagcttgcagagatgctagagcaggaaggtgtagatatcatccagactgaaggaggaaaatgttccaacccctcccaatccggcattcttggtttgatcgagaaggcgtcaccaacattagcagcagcatactctatatcacgggctgtcaacattcctgtcatgtgttcatctggactgagtgcagttacagcaccaatggccataacagcaggagcagcgggtgtgggcgttggctcagcagttaacaagctcaatgatgtagttgcgatgattgcagaagtcaggagtatctccgattcattaggagcatcagctggtaccggtaggcgtagtaccagcgaagaaaaaactttgaaactgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF561

Protein Analysis

307

Amino Acids

32.07

Weight (kDa)

6.24

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016283)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05890 FvH4_3g05890
malus_domestica MD05G1286900.v1.1
prunus_persica Prupe.4G078000_v2.0.a1
pyrus_communis pycom05g26530
rosa_chinensis RchiOBHm_Chr5g0013551
rosa_laevigata RLG00000032027
rosa_multiflora Rmu_sc0002573.1_g000021 Rmu_sc0003458.1_g000024
rosa_roxburghii Rroxscaffold_1G00063020
rosa_rugosa Rorug05G0010900
rosa_samantha Rh5AG105500 Rh5CG113600 Rh5DG100300
rosa_wichuraiana Rw5G009190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 878
AccB1I GGYRCC 1 cut(s) 878
AciI CCGC 2 cut(s) 187, 779
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 759
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 665
AfaI GTAC 3 cut(s) 528, 880, 895
AfiI CCNNNNNNNGG 3 cut(s) 73, 74, 307
AgeI ACCGGT 1 cut(s) 881
AgsI TTSAA 4 cut(s) 93, 172, 389, 916
AjnI CCWGG 1 cut(s) 552
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 7 cut(s) 194, 293, 316, 380, 562, 811, 875
AluI AGCT 7 cut(s) 194, 293, 316, 380, 562, 811, 875
Alw21I GWGCWC 2 cut(s) 196, 400
AlwI GGATC 1 cut(s) 356
AlwNI CAGNNNCTG 2 cut(s) 383, 524
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 3 cut(s) 6, 510, 759
ApeKI GCWGC 5 cut(s) 257, 380, 680, 683, 776
ApoI RAATTY 1 cut(s) 220
AsiGI ACCGGT 1 cut(s) 881
Asp700I GAANNNNTTC 1 cut(s) 619
Asp718I GGTACC 1 cut(s) 878
AsuHPI GGTGA 3 cut(s) 121, 322, 660
AvaI CYCGRG 1 cut(s) 365
BalI TGGCCA 1 cut(s) 761
BanI GGYRCC 1 cut(s) 878
BanII GRGCYC 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 196, 400
BbvI GCAGC 5 cut(s) 244, 367, 692, 695, 788
BccI CCATC 2 cut(s) 433, 511
BciT130I CCWGG 1 cut(s) 554
BfaI CTAG 2 cut(s) 476, 575
BfmI CTRYAG 2 cut(s) 258, 357
BisI GCNGC 5 cut(s) 258, 381, 681, 684, 777
BlpI GCTNAGC 1 cut(s) 796
BlsI GCNGC 5 cut(s) 259, 382, 682, 685, 778
Bme1390I CCNGG 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 880
BmrFI CCNGG 1 cut(s) 554
BmsI GCATC 4 cut(s) 115, 251, 561, 878
BpiI GAAGAC 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 796
BpuEI CTTGAG 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 881
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 74, 307
Bse118I RCCGGY 1 cut(s) 881
Bse1I ACTGG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 554
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 597
BseLI CCNNNNNNNGG 3 cut(s) 73, 74, 307
BseMII CTCAG 4 cut(s) 294, 450, 728, 810
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 5 cut(s) 244, 367, 692, 695, 788
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 3 cut(s) 8, 512, 761
BshNI GGYRCC 1 cut(s) 878
BshTI ACCGGT 1 cut(s) 881
BsiHKAI GWGCWC 2 cut(s) 196, 400
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 2 cut(s) 639, 882
BslI CCNNNNNNNGG 3 cut(s) 73, 74, 307
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 3 cut(s) 8, 512, 761
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 196, 400
Bsp143I GATC 2 cut(s) 361, 654
Bsp1720I GCTNAGC 1 cut(s) 796
BspACI CCGC 2 cut(s) 187, 779
BspANI GGCC 3 cut(s) 8, 512, 761
BspCNI CTCAG 4 cut(s) 295, 451, 729, 809
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 880
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 1 cut(s) 356
BspQI GCTCTTC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 878
BsrFI RCCGGY 1 cut(s) 881
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 2 cut(s) 361, 654
BssNI GRCGYC 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 2 cut(s) 526, 921
Bst6I CTCTTC 3 cut(s) 75, 112, 342
BstACI GRCGYC 1 cut(s) 665
BstC8I GCNNGC 4 cut(s) 6, 104, 318, 564
BstDEI CTNAG 5 cut(s) 303, 459, 539, 737, 796
BstF5I GGATG 1 cut(s) 597
BstKTI GATC 2 cut(s) 364, 657
BstMBI GATC 2 cut(s) 361, 654
BstMWI GCNNNNNNNGC 7 cut(s) 377, 389, 749, 758, 767, 776, 833
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 552
BstSFI CTRYAG 2 cut(s) 258, 357
BstV1I GCAGC 5 cut(s) 244, 367, 692, 695, 788
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 3 cut(s) 8, 512, 761
BtgZI GCGATG 1 cut(s) 842
BtsCI GGATG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 522
Cac8I GCNNGC 4 cut(s) 6, 104, 318, 564
CaiI CAGNNNCTG 2 cut(s) 383, 524
CciI TCATGA 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 881
CseI GACGC 1 cut(s) 654
CsiI ACCWGGT 1 cut(s) 552
Csp6I GTAC 3 cut(s) 527, 879, 894
CspAI ACCGGT 1 cut(s) 881
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 4 cut(s) 99, 182, 281, 721
CviQI GTAC 3 cut(s) 527, 879, 894
DdeI CTNAG 5 cut(s) 303, 459, 539, 737, 796
DpnI GATC 2 cut(s) 363, 656
DpnII GATC 2 cut(s) 361, 654
EaeI YGGCCR 1 cut(s) 759
Eam1104I CTCTTC 3 cut(s) 75, 112, 342
EarI CTCTTC 3 cut(s) 75, 112, 342
Ecl136II GAGCTC 1 cut(s) 194
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 552
EcoRV GATATC 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 196
FaeI CATG 4 cut(s) 102, 185, 284, 724
FalI AAGNNNNNCTT 2 cut(s) 109, 141
FatI CATG 4 cut(s) 98, 181, 280, 720
FauI CCCGC 1 cut(s) 772
Fnu4HI GCNGC 5 cut(s) 258, 381, 681, 684, 777
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 5 cut(s) 258, 381, 681, 684, 777
FspBI CTAG 2 cut(s) 476, 575
GluI GCNGC 5 cut(s) 258, 381, 681, 684, 777
HaeIII GGCC 3 cut(s) 8, 512, 761
HapII CCGG 2 cut(s) 639, 882
HgaI GACGC 1 cut(s) 654
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 4 cut(s) 102, 185, 284, 724
HincII GTYRAC 3 cut(s) 150, 709, 805
HindII GTYRAC 3 cut(s) 150, 709, 805
HindIII AAGCTT 2 cut(s) 314, 560
HinfI GANTC 4 cut(s) 161, 425, 496, 857
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 2 cut(s) 639, 882
HphI GGTGA 3 cut(s) 121, 322, 660
Hpy166II GTNNAC 3 cut(s) 150, 709, 805
Hpy188I TCNGA 2 cut(s) 111, 856
Hpy8I GTNNAC 3 cut(s) 150, 709, 805
HpyAV CCTTC 5 cut(s) 56, 554, 578, 602, 655
HpyCH4III ACNGT 2 cut(s) 526, 921
HpyCH4V TGCA 8 cut(s) 4, 106, 217, 242, 260, 566, 743, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 377, 389, 749, 758, 767, 776, 833
HpyF3I CTNAG 5 cut(s) 303, 459, 539, 737, 796
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 4 cut(s) 102, 185, 284, 724
KpnI GGTACC 1 cut(s) 882
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 2 cut(s) 361, 654
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 4 cut(s) 272, 395, 773, 866
Lsp1109I GCAGC 5 cut(s) 244, 367, 692, 695, 788
LweI GCATC 4 cut(s) 115, 251, 561, 878
MabI ACCWGGT 1 cut(s) 552
MaeI CTAG 2 cut(s) 476, 575
MaeIII GTNAC 4 cut(s) 296, 520, 666, 745
MalI GATC 2 cut(s) 363, 656
MboI GATC 2 cut(s) 361, 654
MboII GAAGA 7 cut(s) 62, 81, 129, 329, 345, 491, 914
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 196, 400
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 2 cut(s) 12, 220
MluNI TGGCCA 1 cut(s) 761
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 2 cut(s) 47, 648
MnlI CCTC 9 cut(s) 19, 46, 78, 133, 263, 361, 486, 605, 640
Mox20I TGGCCA 1 cut(s) 761
MroXI GAANNNNTTC 1 cut(s) 619
MscI TGGCCA 1 cut(s) 761
MseI TTAA 2 cut(s) 470, 804
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 3 cut(s) 380, 779, 875
MspI CCGG 2 cut(s) 639, 882
MspR9I CCNGG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 1 cut(s) 554
MwoI GCNNNNNNNGC 7 cut(s) 377, 389, 749, 758, 767, 776, 833
NdeII GATC 2 cut(s) 361, 654
NlaIII CATG 4 cut(s) 102, 185, 284, 724
NlaIV GGNNCC 1 cut(s) 880
NmuCI GTSAC 3 cut(s) 296, 520, 666
PagI TCATGA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 112
PctI GAATGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 619
PfeI GAWTC 3 cut(s) 161, 496, 857
PflFI GACNNNGTC 1 cut(s) 554
PinAI ACCGGT 1 cut(s) 881
PkrI GCNGC 5 cut(s) 259, 382, 682, 685, 778
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 1 cut(s) 880
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 2 cut(s) 383, 524
PsuI RGATCY 1 cut(s) 361
PsyI GACNNNGTC 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 380, 875
RsaI GTAC 3 cut(s) 528, 880, 895
RsaNI GTAC 3 cut(s) 527, 879, 894
SacI GAGCTC 1 cut(s) 196
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 2 cut(s) 470, 804
SatI GCNGC 5 cut(s) 258, 381, 681, 684, 777
Sau3AI GATC 2 cut(s) 361, 654
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 554
SduI GDGCHC 2 cut(s) 196, 400
SexAI ACCWGGT 1 cut(s) 552
SfaNI GCATC 4 cut(s) 115, 251, 561, 878
SfcI CTRYAG 2 cut(s) 258, 357
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 12, 220
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 2 cut(s) 187, 779
SspMI CTAG 2 cut(s) 476, 575
SstI GAGCTC 1 cut(s) 196
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 526, 921
TaqI TCGA 1 cut(s) 657
TasI AATT 2 cut(s) 12, 220
TfiI GAWTC 3 cut(s) 161, 496, 857
Tru1I TTAA 2 cut(s) 470, 804
Tru9I TTAA 2 cut(s) 470, 804
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 3 cut(s) 296, 520, 666
TseI GCWGC 5 cut(s) 257, 380, 680, 683, 776
Tsp45I GTSAC 3 cut(s) 296, 520, 666
TspDTI ATGAA 6 cut(s) 7, 18, 38, 170, 717, 849
TspRI CASTG 1 cut(s) 529
Tth111I GACNNNGTC 1 cut(s) 554
XapI RAATTY 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 619
XspI CTAG 2 cut(s) 476, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.