pycom05g26530

Protein of unknown function (DUF561)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016283
Physical Location & Seq
Reverse (-)
27765427 .. 27767587
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26530.7

Sequence Viewer

Length: 921 bp
ATGCAGGCCTCAATCTGCCCACAAACTTCACCACCCTCTCTATCCAGTTTCAGACCTACCCACCACAACCCTCTTCTGGGTAACTCTCTGCCTTCAAGGCCATGTTTGGCTCTCAAAAAAAGAGCCACTTTAACCACAAAAGCACTTCTGTCAGCTACCAAGGAATCAGTCCTCAGGGACTTTCATGAACGCAGAGCTCTCAAGATTATTTCAGGATTGCAGAATTTTGACAAAGAAAATGTTGCATCAGTCATTACTGCCGCTGAGCAGGGAGGAGCAACTCATGTGGATATAGCCTGTGACCCGGAGTTGGTGAAGCTTGCTATCGATTTAACTTCTCTTCCGGTTTGTGTTTCTTCTGTAGATCCAGCAGCATTTCCAGCTGCCGTTGAAGCAGGAGCACTGATGGTTGAGATTGGAAACTACGACTCCTTTTACGAGATGGGAGTGGTTTTCACTTCGGAGCAGATATTGAATCTGACAAAGGAGACCAAGAGGATTCTTCCGTCTGTGACCTTATCAGTCACTGTACCTCATACACTAAGCCTTCCAGATCAGGTCAAGCTTGCAGAGATGCTGGAGCAGGCAGGTGTCGACATCATTCAAACCGAAGGAGGAAAATGTACCAGTCCTTCGAAATCAGGTGTTCTTGGTTTGGTCGAGAAGGCAACACCAACATTGGCAGCAGCATATTCGATATCCCATGCTGTCAAGATTCCGGTAATGTGTTCATCCGGATTGAGTGCAGTTACAGCCCCAATGGCAATAGCAGCAGGAGCTGCCGGTGTGGGCGTCGGCTCAGCCGTGAACAAGCTCAATGATGTTGTTGCAATGATTGCAGAAGTGAGGAGTATTTCCAATTCATTAGGAGCTTCAGCTGGTAGGCTCACTGCAGCTGAAGAACAAGTTTTGAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF561

Protein Analysis

307

Amino Acids

31.78

Weight (kDa)

6.31

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016283)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05890 FvH4_3g05890
malus_domestica MD05G1286900.v1.1
prunus_persica Prupe.4G078000_v2.0.a1
pyrus_communis pycom05g26530
rosa_chinensis RchiOBHm_Chr5g0013551
rosa_laevigata RLG00000032027
rosa_multiflora Rmu_sc0002573.1_g000021 Rmu_sc0003458.1_g000024
rosa_roxburghii Rroxscaffold_1G00063020
rosa_rugosa Rorug05G0010900
rosa_samantha Rh5AG105500 Rh5CG113600 Rh5DG100300
rosa_wichuraiana Rw5G009190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 578
Acc36I ACCTGC 1 cut(s) 578
AccI GTMKAC 1 cut(s) 594
AccIII TCCGGA 1 cut(s) 734
AciI CCGC 1 cut(s) 261
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 858, 918
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 2 cut(s) 531, 625
AfiI CCNNNNNNNGG 3 cut(s) 76, 77, 310
AgsI TTSAA 5 cut(s) 96, 392, 475, 605, 913
Alw21I GWGCWC 2 cut(s) 199, 403
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 2 cut(s) 527, 779
Aor13HI TCCGGA 1 cut(s) 734
AoxI GGCC 2 cut(s) 6, 98
ApeKI GCWGC 7 cut(s) 371, 383, 683, 686, 770, 779, 893
ApoI RAATTY 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 21, 325
AsuII TTCGAA 1 cut(s) 635
AxyI CCTNAGG 1 cut(s) 173
BanII GRGCYC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
Bbv12I GWGCWC 2 cut(s) 199, 403
BbvI GCAGC 7 cut(s) 370, 383, 695, 698, 766, 782, 905
BccI CCATC 2 cut(s) 400, 436
BceAI ACGGC 2 cut(s) 371, 788
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 1 cut(s) 482
BfmI CTRYAG 2 cut(s) 360, 891
BfuAI ACCTGC 1 cut(s) 578
BglI GCCNNNNNGGC 2 cut(s) 97, 761
BisI GCNGC 8 cut(s) 261, 372, 384, 684, 687, 771, 780, 894
BlpI GCTNAGC 2 cut(s) 264, 799
BlsI GCNGC 8 cut(s) 262, 373, 385, 685, 688, 772, 781, 895
Bme1390I CCNGG 1 cut(s) 305
BmrFI CCNGG 1 cut(s) 305
BmsI GCATC 2 cut(s) 254, 564
BpmI CTGGAG 1 cut(s) 599
Bpu1102I GCTNAGC 2 cut(s) 264, 799
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 3 cut(s) 343, 718, 734
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 77, 310
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 2 cut(s) 45, 627
Bse21I CCTNAGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 837
BseAI TCCGGA 1 cut(s) 734
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 731
BseLI CCNNNNNNNGG 3 cut(s) 76, 77, 310
BseMI GCAATG 1 cut(s) 837
BseMII CTCAG 3 cut(s) 187, 255, 813
BseNI ACTGG 2 cut(s) 45, 627
BseRI GAGGAG 2 cut(s) 288, 862
BseXI GCAGC 7 cut(s) 370, 383, 695, 698, 766, 782, 905
BsgI GTGCAG 1 cut(s) 765
BshFI GGCC 2 cut(s) 8, 100
BshVI ATCGAT 1 cut(s) 327
BsiHKAI GWGCWC 2 cut(s) 199, 403
BsiSI CCGG 5 cut(s) 305, 344, 719, 735, 783
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 3 cut(s) 76, 77, 310
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 2 cut(s) 8, 100
Bso31I GGTCTC 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 199, 403
Bsp13I TCCGGA 1 cut(s) 734
Bsp143I GATC 2 cut(s) 364, 553
Bsp1720I GCTNAGC 2 cut(s) 264, 799
BspACI CCGC 1 cut(s) 261
BspANI GGCC 2 cut(s) 8, 100
BspCNI CTCAG 3 cut(s) 186, 256, 812
BspDI ATCGAT 1 cut(s) 327
BspEI TCCGGA 1 cut(s) 734
BspHI TCATGA 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 895
BspMI ACCTGC 1 cut(s) 578
BspPI GGATC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 2 cut(s) 45, 627
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 364, 553
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 529, 918
Bst6I CTCTTC 2 cut(s) 78, 345
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 2 cut(s) 779, 836
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 6, 321, 567, 585
BstDEI CTNAG 4 cut(s) 173, 264, 542, 799
BstF5I GGATG 1 cut(s) 731
BstKTI GATC 2 cut(s) 367, 556
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 2 cut(s) 364, 553
BstMWI GCNNNNNNNGC 9 cut(s) 97, 380, 392, 752, 761, 770, 776, 779, 836
BstSCI CCNGG 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 360, 891
BstV1I GCAGC 7 cut(s) 370, 383, 695, 698, 766, 782, 905
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 327
Bsu36I CCTNAGG 1 cut(s) 173
BsuRI GGCC 2 cut(s) 8, 100
BsuTUI ATCGAT 1 cut(s) 327
BtsCI GGATG 1 cut(s) 731
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 3 cut(s) 401, 525, 888
BveI ACCTGC 1 cut(s) 578
Cac8I GCNNGC 4 cut(s) 6, 321, 567, 585
CaiI CAGNNNCTG 2 cut(s) 527, 779
CciI TCATGA 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 782
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 1 cut(s) 781
Csp6I GTAC 2 cut(s) 530, 624
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 4 cut(s) 102, 185, 284, 704
CviQI GTAC 2 cut(s) 530, 624
DdeI CTNAG 4 cut(s) 173, 264, 542, 799
DpnI GATC 2 cut(s) 366, 555
DpnII GATC 2 cut(s) 364, 553
Eam1104I CTCTTC 2 cut(s) 78, 345
EarI CTCTTC 2 cut(s) 78, 345
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 482
Eco32I GATATC 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 858, 918
Eco81I CCTNAGG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 197
EcoRV GATATC 1 cut(s) 699
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 4 cut(s) 105, 188, 287, 707
FaiI YATR 7 cut(s) 103, 186, 285, 293, 537, 691, 705
FalI AAGNNNNNCTT 2 cut(s) 112, 144
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 101, 184, 283, 703
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 8 cut(s) 261, 372, 384, 684, 687, 771, 780, 894
FokI GGATG 1 cut(s) 718
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 8 cut(s) 261, 372, 384, 684, 687, 771, 780, 894
GluI GCNGC 8 cut(s) 261, 372, 384, 684, 687, 771, 780, 894
GsuI CTGGAG 1 cut(s) 599
HaeIII GGCC 2 cut(s) 8, 100
HapII CCGG 5 cut(s) 305, 344, 719, 735, 783
HgaI GACGC 1 cut(s) 781
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 4 cut(s) 105, 188, 287, 707
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 2 cut(s) 317, 563
HinfI GANTC 5 cut(s) 164, 428, 475, 499, 715
HpaII CCGG 5 cut(s) 305, 344, 719, 735, 783
HphI GGTGA 2 cut(s) 21, 325
Hpy166II GTNNAC 2 cut(s) 595, 808
Hpy188I TCNGA 3 cut(s) 53, 463, 480
Hpy188III TCNNGA 7 cut(s) 185, 202, 213, 551, 661, 712, 735
Hpy8I GTNNAC 2 cut(s) 595, 808
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 5 cut(s) 102, 557, 605, 642, 658
HpyCH4III ACNGT 2 cut(s) 529, 918
HpyCH4V TGCA 8 cut(s) 4, 220, 245, 569, 746, 830, 839, 893
HpyF10VI GCNNNNNNNGC 9 cut(s) 97, 380, 392, 752, 761, 770, 776, 779, 836
HpyF3I CTNAG 4 cut(s) 173, 264, 542, 799
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 4 cut(s) 105, 188, 287, 707
Kpn2I TCCGGA 1 cut(s) 734
Kzo9I GATC 2 cut(s) 364, 553
LmnI GCTCC 6 cut(s) 275, 398, 463, 580, 776, 869
Lsp1109I GCAGC 7 cut(s) 370, 383, 695, 698, 766, 782, 905
LweI GCATC 2 cut(s) 254, 564
MaeIII GTNAC 5 cut(s) 80, 299, 511, 523, 748
MalI GATC 2 cut(s) 366, 555
MboI GATC 2 cut(s) 364, 553
MboII GAAGA 5 cut(s) 65, 332, 348, 494, 911
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 2 cut(s) 199, 403
MluCI AATT 2 cut(s) 223, 859
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 9 cut(s) 19, 46, 81, 182, 266, 489, 543, 608, 840
MroI TCCGGA 1 cut(s) 734
MseI TTAA 2 cut(s) 131, 332
MspA1I CMGCKG 4 cut(s) 263, 383, 878, 896
MspI CCGG 5 cut(s) 305, 344, 719, 735, 783
MspR9I CCNGG 1 cut(s) 305
MwoI GCNNNNNNNGC 9 cut(s) 97, 380, 392, 752, 761, 770, 776, 779, 836
NciI CCSGG 1 cut(s) 305
NdeII GATC 2 cut(s) 364, 553
NlaIII CATG 4 cut(s) 105, 188, 287, 707
NmuCI GTSAC 3 cut(s) 299, 511, 523
NspV TTCGAA 1 cut(s) 635
PagI TCATGA 1 cut(s) 184
PaqCI CACCTGC 1 cut(s) 578
PceI AGGCCT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 801
PfeI GAWTC 4 cut(s) 164, 475, 499, 715
PkrI GCNGC 8 cut(s) 262, 373, 385, 685, 688, 772, 781, 895
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 199
PstI CTGCAG 1 cut(s) 895
PstNI CAGNNNCTG 2 cut(s) 527, 779
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 3 cut(s) 383, 878, 896
RsaI GTAC 2 cut(s) 531, 625
RsaNI GTAC 2 cut(s) 530, 624
SacI GAGCTC 1 cut(s) 199
SalI GTCGAC 1 cut(s) 593
SaqAI TTAA 2 cut(s) 131, 332
SatI GCNGC 8 cut(s) 261, 372, 384, 684, 687, 771, 780, 894
Sau3AI GATC 2 cut(s) 364, 553
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 2 cut(s) 199, 403
SfaNI GCATC 2 cut(s) 254, 564
SfcI CTRYAG 2 cut(s) 360, 891
SfuI TTCGAA 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 223, 859
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 1 cut(s) 261
SstI GAGCTC 1 cut(s) 199
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 529, 918
TaqI TCGA 5 cut(s) 327, 594, 635, 660, 695
TasI AATT 2 cut(s) 223, 859
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 4 cut(s) 164, 475, 499, 715
Tru1I TTAA 2 cut(s) 131, 332
Tru9I TTAA 2 cut(s) 131, 332
TscAI CASTG 3 cut(s) 408, 532, 895
TseFI GTSAC 3 cut(s) 299, 511, 523
TseI GCWGC 7 cut(s) 371, 383, 683, 686, 770, 779, 893
Tsp45I GTSAC 3 cut(s) 299, 511, 523
TspDTI ATGAA 4 cut(s) 173, 201, 720, 852
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 3 cut(s) 408, 532, 895
XapI RAATTY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.