FvH4_3g20530

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008686
Physical Location & Seq
Forward (+)
13581589 .. 13587240
5652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20530.t3

Sequence Viewer

Length: 1476 bp
ATGGCGGGATATAGGCCTGTTACGATTGAGGACTTGCCGACACATTTGGTTTTGGAAATCTTGTGCTCCGGGAGGCTTAGTGCTGTTGATCTTTTGCATTTAGAGACGACTTCTAGGACGTTTGGTGGAAGTCAGGGGTTGTATCCTCATAAGTTTCGGTCATTGGTAGATTACACAGCGTTTCAGCTGTGTGTGTCGCATTCTGTATATGCTAAGATGGAGAAGAATGCTCAGACAGTGCTGTTTGATCGGTGTGGTGGGAATTGGAAGCGGATTTTGAGGTTCTTGCAATCTGTGGAACAATCATCTGACATGGTGGAGACTTCAGCAGGCAATATGCATATTACCACTGGAAGGTATCACACTTTGCTTATAAGCAATTCATCAGTCTACTCGTGTGGTTCCAGCTTGTGTGGTGTTCTTGGCCAAGGTGCCAAGACTAAACTATGTGTGACATTTACCCGGATTAGTTTTCCATCTCGAGCACATGTTGTCCAAGTCTCAGCCTCCCAGAACCATGCTGCTTTTGTTATGCATTCCGGAGAGGTTTTCACATGTGGGGACAATTCGTCATCTTGTTGTGGGCATAAAGATACAAACCGGCCAATATTTAGGCCTAGGCTCGTAGAGGCAATGAAGGGAATACCTTGCAAGCAGGTAGTTGCAGGGCTTAATTTTACAGCTTTCCTTACAAGGCAAGGCCATGTTTATACATGTGGATCCAATACACATGGACAACTTGGACATGGAGACATTACAGATAGGCCAACTCCCAAACTTATTGAATTTCTCAAGCAAATTGGCTCCATAGTACAGATTGCTGCTGGTCCGAGCTACGTCCTAGCTGTAACTGACAATGGAGTAGTATACTCTTTTGGGACAGGTTCTAATTTCTGTCTCGGCCATGGAGAGCAACATCATGAGTTCCAACCTCGTGCAATCCAATCATTTAGAAGGAAGGGTATTCATGTAGTTCGAGTCTCTGCTGGTGACGAGCATGCAGTGGCACTTGATTCCAGTGGATTTGTATATACCTGGGGTAAAGGGTACTGTGGTGCATTAGGCCATGGGGATGAGATTGACAAGACCACCCCGGAACTCTTGAGCAGAGTTCAAAGCCAGCTTGCCGTGCAGGTGTGTGCAAGAAAACGGAAGACCTTTGTTCTGATGGATGATGGTTTAGTATATGGTTGTGGTTGGATGGGGTTTGGTAGCCTTGGGTTTACAGACAGGGGACTATCTGACAAAATCTTGAGCCCTCGAATCCTTGATAGCTTAAGACCTCATCATGTTTCCCAGATTAGCACTGGCCTCTACCATACTGTTGCTATCACTAACTCTGGACAACTCTTTGGATTTGGAGATAATGAAAGAGCACAACTTGGGCACGATACACTGAGAGCATGCCTCAAACCAACTGAAATTTTTATAGAAGAAATGACTGATGAGGTTGATCTTAATTCCGAAACCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

492

Amino Acids

53.65

Weight (kDa)

7.89

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 111 - 317 6.9e-44 RCC1-like domain
RCC1 PF00415 180 - 230 1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 218 - 246 3.3e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 233 - 283 9.6e-14 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 284 - 483 7.5e-09 Alsin RCC1-like domain
RCC1 PF00415 286 - 337 1.6e-11 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 309 - 475 5.2e-33 RCC1-like domain
RCC1_2 PF13540 324 - 352 8.9e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 341 - 381 2.4e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 431 - 460 7.2e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AarI CACCTGC 1 cut(s) 1123
Acc36I ACCTGC 2 cut(s) 646, 1123
AccB1I GGYRCC 1 cut(s) 431
AccI GTMKAC 2 cut(s) 390, 867
AccIII TCCGGA 1 cut(s) 539
AciI CCGC 2 cut(s) 5, 271
AclWI GGATC 2 cut(s) 714, 727
AcoI YGGCCR 3 cut(s) 424, 602, 901
AcsI RAATTY 2 cut(s) 785, 1422
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 813, 1049
AfiI CCNNNNNNNGG 1 cut(s) 354
AflII CTTAAG 1 cut(s) 1276
AflIII ACRYGT 3 cut(s) 487, 554, 713
AgsI TTSAA 2 cut(s) 785, 1115
AhdI GACNNNNNGTC 1 cut(s) 568
AjnI CCWGG 1 cut(s) 1034
AluBI AGCT 7 cut(s) 187, 408, 683, 834, 845, 1123, 1275
AluI AGCT 7 cut(s) 187, 408, 683, 834, 845, 1123, 1275
Alw21I GWGCWC 3 cut(s) 68, 487, 1378
Alw26I GTCTC 6 cut(s) 98, 314, 505, 744, 902, 985
AlwI GGATC 2 cut(s) 714, 727
Ama87I CYCGRG 1 cut(s) 480
Aor13HI TCCGGA 1 cut(s) 539
AoxI GGCC 9 cut(s) 14, 424, 602, 614, 700, 764, 901, 1063, 1309
ApeKI GCWGC 2 cut(s) 521, 821
ApoI RAATTY 2 cut(s) 785, 1422
AspA2I CCTAGG 1 cut(s) 617
AspS9I GGNCC 1 cut(s) 827
AsuC2I CCSGG 3 cut(s) 70, 463, 1094
AsuHPI GGTGA 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 1 cut(s) 827
AvrII CCTAGG 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 1389
BaeI ACNNNNGTAYC 2 cut(s) 1039, 1072
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 719
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 1259
BauI CACGAG 2 cut(s) 394, 933
BbsI GAAGAC 1 cut(s) 1160
Bbv12I GWGCWC 3 cut(s) 68, 487, 1378
BbvI GCAGC 2 cut(s) 508, 808
BccI CCATC 5 cut(s) 211, 484, 1162, 1169, 1195
BceAI ACGGC 1 cut(s) 1112
BciT130I CCWGG 1 cut(s) 1036
BciVI GTATCC 1 cut(s) 153
BcnI CCSGG 3 cut(s) 70, 463, 1094
BcoDI GTCTC 6 cut(s) 98, 314, 505, 744, 902, 985
BfaI CTAG 3 cut(s) 114, 618, 842
BfrI CTTAAG 1 cut(s) 1276
BfuAI ACCTGC 2 cut(s) 646, 1123
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 2 cut(s) 522, 822
BlnI CCTAGG 1 cut(s) 617
BlsI GCNGC 2 cut(s) 523, 823
Bme1390I CCNGG 4 cut(s) 70, 463, 1036, 1094
Bme18I GGWCC 1 cut(s) 827
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 827
BmiI GGNNCC 4 cut(s) 403, 433, 721, 805
BmrFI CCNGG 4 cut(s) 70, 463, 1036, 1094
BpiI GAAGAC 1 cut(s) 1160
BplI GAGNNNNNCTC 2 cut(s) 1392, 1424
BpuEI CTTGAG 3 cut(s) 776, 1123, 1273
BpuMI CCSGG 3 cut(s) 70, 463, 1094
BsaJI CCNNGG 7 cut(s) 427, 617, 904, 1035, 1066, 1092, 1216
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse118I RCCGGY 1 cut(s) 600
Bse1I ACTGG 3 cut(s) 355, 1017, 1312
Bse3DI GCAATG 1 cut(s) 639
BseAI TCCGGA 1 cut(s) 539
BseBI CCWGG 1 cut(s) 1036
BseDI CCNNGG 7 cut(s) 427, 617, 904, 1035, 1066, 1092, 1216
BseGI GGATG 3 cut(s) 1078, 1177, 1206
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 245, 516, 1388
BseNI ACTGG 3 cut(s) 355, 1017, 1312
BseSI GKGCMC 1 cut(s) 1389
BseXI GCAGC 2 cut(s) 508, 808
BsgI GTGCAG 1 cut(s) 1151
BshFI GGCC 9 cut(s) 16, 426, 604, 616, 702, 766, 903, 1065, 1311
BshNI GGYRCC 1 cut(s) 431
BsiHKAI GWGCWC 3 cut(s) 68, 487, 1378
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 5 cut(s) 69, 463, 540, 601, 1094
BslFI GGGAC 3 cut(s) 575, 892, 1248
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 6 cut(s) 98, 314, 505, 744, 902, 985
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 3 cut(s) 575, 892, 1248
BsmI GAATGC 3 cut(s) 199, 232, 535
BsnI GGCC 9 cut(s) 16, 426, 604, 616, 702, 766, 903, 1065, 1311
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 5 cut(s) 68, 487, 1259, 1378, 1389
Bsp13I TCCGGA 1 cut(s) 539
Bsp143I GATC 4 cut(s) 88, 247, 719, 1453
Bsp19I CCATGG 2 cut(s) 904, 1066
BspACI CCGC 2 cut(s) 5, 271
BspANI GGCC 9 cut(s) 16, 426, 604, 616, 702, 766, 903, 1065, 1311
BspCNI CTCAG 3 cut(s) 244, 515, 1389
BspEI TCCGGA 1 cut(s) 539
BspHI TCATGA 1 cut(s) 919
BspLI GGNNCC 4 cut(s) 403, 433, 721, 805
BspMI ACCTGC 2 cut(s) 646, 1123
BspPI GGATC 2 cut(s) 714, 727
BspT107I GGYRCC 1 cut(s) 431
BspTI CTTAAG 1 cut(s) 1276
BsrDI GCAATG 1 cut(s) 639
BsrFI RCCGGY 1 cut(s) 600
BsrI ACTGG 3 cut(s) 355, 1017, 1312
BssAI RCCGGY 1 cut(s) 600
BssECI CCNNGG 7 cut(s) 427, 617, 904, 1035, 1066, 1092, 1216
BssMI GATC 4 cut(s) 88, 247, 719, 1453
BssNAI GTATAC 1 cut(s) 868
BssSI CACGAG 2 cut(s) 394, 933
BssT1I CCWWGG 5 cut(s) 427, 617, 904, 1066, 1216
Bst1107I GTATAC 1 cut(s) 868
Bst2BI CACGAG 2 cut(s) 394, 933
Bst2UI CCWGG 1 cut(s) 1036
Bst4CI ACNGT 3 cut(s) 238, 1052, 1324
BstAFI CTTAAG 1 cut(s) 1276
BstC8I GCNNGC 6 cut(s) 331, 653, 999, 1121, 1125, 1405
BstDEI CTNAG 5 cut(s) 77, 213, 231, 502, 1397
BstDSI CCRYGG 2 cut(s) 904, 1066
BstF5I GGATG 3 cut(s) 1078, 1177, 1206
BstKTI GATC 4 cut(s) 91, 250, 722, 1456
BstMAI GTCTC 6 cut(s) 98, 314, 505, 744, 902, 985
BstMBI GATC 4 cut(s) 88, 247, 719, 1453
BstMWI GCNNNNNNNGC 1 cut(s) 1129
BstNI CCWGG 1 cut(s) 1036
BstNSI RCATGY 5 cut(s) 491, 558, 717, 1001, 1407
BstSCI CCNGG 4 cut(s) 68, 461, 1034, 1092
BstSLI GKGCMC 1 cut(s) 1389
BstV1I GCAGC 2 cut(s) 508, 808
BstV2I GAAGAC 1 cut(s) 1160
BstX2I RGATCY 1 cut(s) 719
BstYI RGATCY 1 cut(s) 719
BstZ17I GTATAC 1 cut(s) 868
BsuI GTATCC 1 cut(s) 153
BsuRI GGCC 9 cut(s) 16, 426, 604, 616, 702, 766, 903, 1065, 1311
BtgI CCRYGG 2 cut(s) 904, 1066
BtsCI GGATG 3 cut(s) 1078, 1177, 1206
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 6 cut(s) 243, 348, 1008, 1024, 1305, 1394
BveI ACCTGC 2 cut(s) 646, 1123
Cac8I GCNNGC 6 cut(s) 331, 653, 999, 1121, 1125, 1405
CciI TCATGA 1 cut(s) 919
Cfr10I RCCGGY 1 cut(s) 600
Cfr13I GGNCC 1 cut(s) 827
Csp6I GTAC 2 cut(s) 812, 1048
CviQI GTAC 2 cut(s) 812, 1048
DdeI CTNAG 5 cut(s) 77, 213, 231, 502, 1397
DpnI GATC 4 cut(s) 90, 249, 721, 1455
DpnII GATC 4 cut(s) 88, 247, 719, 1453
DriI GACNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 3 cut(s) 424, 602, 901
Eam1105I GACNNNNNGTC 1 cut(s) 568
Eco130I CCWWGG 5 cut(s) 427, 617, 904, 1066, 1216
Eco147I AGGCCT 2 cut(s) 16, 616
Eco24I GRGCYC 1 cut(s) 1259
Eco47I GGWCC 1 cut(s) 827
Eco57I CTGAAG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 1034
EcoT14I CCWWGG 5 cut(s) 427, 617, 904, 1066, 1216
EcoT22I ATGCAT 2 cut(s) 342, 537
EcoT38I GRGCYC 1 cut(s) 1259
ErhI CCWWGG 5 cut(s) 427, 617, 904, 1066, 1216
Esp3I CGTCTC 1 cut(s) 98
FaqI GGGAC 3 cut(s) 575, 892, 1248
FblI GTMKAC 2 cut(s) 390, 867
Fnu4HI GCNGC 2 cut(s) 522, 822
FokI GGATG 3 cut(s) 1085, 1184, 1213
FriOI GRGCYC 1 cut(s) 1259
Fsp4HI GCNGC 2 cut(s) 522, 822
FspBI CTAG 3 cut(s) 114, 618, 842
GluI GCNGC 2 cut(s) 522, 822
HaeIII GGCC 9 cut(s) 16, 426, 604, 616, 702, 766, 903, 1065, 1311
HapII CCGG 5 cut(s) 69, 463, 540, 601, 1094
HinfI GANTC 3 cut(s) 978, 1013, 1263
HpaII CCGG 5 cut(s) 69, 463, 540, 601, 1094
HphI GGTGA 1 cut(s) 1001
Hpy166II GTNNAC 3 cut(s) 391, 868, 1224
Hpy188I TCNGA 7 cut(s) 234, 310, 831, 1167, 1243, 1465, 1475
Hpy188III TCNNGA 6 cut(s) 480, 540, 920, 1102, 1252, 1341
Hpy8I GTNNAC 3 cut(s) 391, 868, 1224
HpyAV CCTTC 4 cut(s) 348, 631, 948, 952
HpyCH4III ACNGT 3 cut(s) 238, 1052, 1324
HpyCH4IV ACGT 2 cut(s) 119, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 1129
HpyF3I CTNAG 5 cut(s) 77, 213, 231, 502, 1397
HpySE526I ACGT 2 cut(s) 119, 837
Kpn2I TCCGGA 1 cut(s) 539
Kzo9I GATC 4 cut(s) 88, 247, 719, 1453
LmnI GCTCC 2 cut(s) 71, 809
Lsp1109I GCAGC 2 cut(s) 508, 808
MaeI CTAG 3 cut(s) 114, 618, 842
MaeII ACGT 2 cut(s) 119, 837
MaeIII GTNAC 4 cut(s) 19, 451, 847, 989
MalI GATC 4 cut(s) 90, 249, 721, 1455
MboI GATC 4 cut(s) 88, 247, 719, 1453
MboII GAAGA 3 cut(s) 235, 1165, 1445
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 5 cut(s) 68, 487, 1259, 1378, 1389
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 9 cut(s) 262, 379, 565, 673, 785, 798, 889, 1422, 1459
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 1 cut(s) 987
MmeI TCCRAC 2 cut(s) 952, 1178
Mox20I TGGCCA 1 cut(s) 426
Mph1103I ATGCAT 2 cut(s) 342, 537
MroI TCCGGA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 426
MseI TTAA 3 cut(s) 672, 1277, 1458
MslI CAYNNNNRTG 1 cut(s) 1071
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 187
MspCI CTTAAG 1 cut(s) 1276
MspI CCGG 5 cut(s) 69, 463, 540, 601, 1094
MspR9I CCNGG 4 cut(s) 70, 463, 1036, 1094
Mva1269I GAATGC 3 cut(s) 199, 232, 535
MvaI CCWGG 1 cut(s) 1036
MwoI GCNNNNNNNGC 1 cut(s) 1129
NciI CCSGG 3 cut(s) 70, 463, 1094
NcoI CCATGG 2 cut(s) 904, 1066
NdeII GATC 4 cut(s) 88, 247, 719, 1453
NlaIV GGNNCC 4 cut(s) 403, 433, 721, 805
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 2 cut(s) 451, 989
NsiI ATGCAT 2 cut(s) 342, 537
NspI RCATGY 5 cut(s) 491, 558, 717, 1001, 1407
PaeI GCATGC 2 cut(s) 1001, 1407
PaeR7I CTCGAG 1 cut(s) 480
PagI TCATGA 1 cut(s) 919
PaqCI CACCTGC 1 cut(s) 1123
PceI AGGCCT 2 cut(s) 16, 616
PciI ACATGT 3 cut(s) 487, 554, 713
PctI GAATGC 3 cut(s) 199, 232, 535
PfeI GAWTC 2 cut(s) 1013, 1263
PfoI TCCNGGA 1 cut(s) 68
PkrI GCNGC 2 cut(s) 523, 823
PleI GAGTC 1 cut(s) 986
PpsI GAGTC 1 cut(s) 986
PscI ACATGT 3 cut(s) 487, 554, 713
PsiI TTATAA 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 1034
PspGI CCWGG 1 cut(s) 1034
PspN4I GGNNCC 4 cut(s) 403, 433, 721, 805
PspPI GGNCC 1 cut(s) 827
PsuI RGATCY 1 cut(s) 719
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 2 cut(s) 813, 1049
RsaNI GTAC 2 cut(s) 812, 1048
RseI CAYNNNNRTG 1 cut(s) 1071
SaqAI TTAA 3 cut(s) 672, 1277, 1458
SatI GCNGC 2 cut(s) 522, 822
Sau3AI GATC 4 cut(s) 88, 247, 719, 1453
Sau96I GGNCC 1 cut(s) 827
SchI GAGTC 1 cut(s) 987
ScrFI CCNGG 4 cut(s) 70, 463, 1036, 1094
SduI GDGCHC 5 cut(s) 68, 487, 1259, 1378, 1389
Sfr274I CTCGAG 1 cut(s) 480
SinI GGWCC 1 cut(s) 827
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 1071
SmlI CTYRAG 5 cut(s) 480, 791, 1102, 1252, 1276
SmoI CTYRAG 5 cut(s) 480, 791, 1102, 1252, 1276
SphI GCATGC 2 cut(s) 1001, 1407
Sse9I AATT 9 cut(s) 262, 379, 565, 673, 785, 798, 889, 1422, 1459
SseBI AGGCCT 2 cut(s) 16, 616
SsiI CCGC 2 cut(s) 5, 271
SspI AATATT 1 cut(s) 609
SspMI CTAG 3 cut(s) 114, 618, 842
StuI AGGCCT 2 cut(s) 16, 616
StyD4I CCNGG 4 cut(s) 68, 461, 1034, 1092
StyI CCWWGG 5 cut(s) 427, 617, 904, 1066, 1216
TaaI ACNGT 3 cut(s) 238, 1052, 1324
TaiI ACGT 2 cut(s) 122, 840
TaqI TCGA 3 cut(s) 481, 976, 1261
TaqII GACCGA 1 cut(s) 147
TasI AATT 9 cut(s) 262, 379, 565, 673, 785, 798, 889, 1422, 1459
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 2 cut(s) 1013, 1263
Tru1I TTAA 3 cut(s) 672, 1277, 1458
Tru9I TTAA 3 cut(s) 672, 1277, 1458
TscAI CASTG 6 cut(s) 243, 355, 1008, 1024, 1312, 1401
TseFI GTSAC 2 cut(s) 451, 989
TseI GCWGC 2 cut(s) 521, 821
Tsp45I GTSAC 2 cut(s) 451, 989
TspDTI ATGAA 4 cut(s) 372, 650, 956, 1383
TspGWI ACGGA 1 cut(s) 1165
TspRI CASTG 6 cut(s) 243, 355, 1008, 1024, 1312, 1401
Vha464I CTTAAG 1 cut(s) 1276
VpaK11BI GGWCC 1 cut(s) 827
XapI RAATTY 2 cut(s) 785, 1422
XceI RCATGY 5 cut(s) 491, 558, 717, 1001, 1407
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XhoI CTCGAG 1 cut(s) 480
XmaJI CCTAGG 1 cut(s) 617
XmiI GTMKAC 2 cut(s) 390, 867
XspI CTAG 3 cut(s) 114, 618, 842
Zsp2I ATGCAT 2 cut(s) 342, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.