FvH4_3g44710

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018016
Physical Location & Seq
Forward (+)
37233380 .. 37234904
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44710.t1

Sequence Viewer

Length: 1356 bp
ATGGTTTCCCGCCAAATTCAAGCCCTAATTCACCGATCGAAATCAGCGGCTCATCTTCTCCAGCTCCACTCTCCGGTCGTCAAAACGGGTCTTGATCACAACCCAAATTTCGTCTCCGATTATATCAACTTAGCGAGCTCGATTTCAATCGAGTTTGCTAAATTGGTGTTTGGTTATGTACCGATATGTCCTCCGCTTTTTGCATGGAATTCGATTATTAGGGTTTATGCAAAGAGCTCGGTTCCGATTGAATCTGTCAGATTCTTCTCTGAGCTGCACAGAGTTGGGCTTAAAGGTGACAACTTTACTTACCCTTTTGTTGTGAAGGCCTGCGGTCGATGTGGGATGGTTGGAGGTGGTGGGATGCTGCAGTGTTTGATTATGAAATCTGGTTTCGATTCGGATCGGTATATAGGGAATGAGGTTTTCAGAATGTATGCTGATTGCGGTGAGGTTGGGATTGCGCGGAAGGTGTTTGAGGAAATGCCTGTGAGAGATGTGGTTTCTTGGAGTTCTTTGATTGCCGGATACGTTGCTTGCAACTACCCATTGGAAGCCTTGAGGGTATTTCGAGAGATGAAGCTAGCTAATGAAAACCCAAACTCGGTCACTTTGGTAAGCTTGCTTACTGCGTGTACCCGTCTACACGATATTAGCATAGGGAAGTCGATTCATTGCTACATTATCGTCCATCCCATAGAGATAGATGTTGCTTTGGGAACTGCTCTGGTCGAGATGTATTCTAAGTGTGGGCATGTTGAGAAAGCCTTCCATATGTTCAACTCAATGAGTGAAAAGAATTTGCAGTCTTGGACTATTATGATTTGTGGTCTTGCCGATCATGGTCGTGGAAACGATTCTATCTTGTTGTTTAACCACATGGAACAAGCTGGACTGGTACCCGACAGCATGGCATTTTCTGCTATCTTGTCAGCCTGTAGCCACTTAGGCCTCCTAAGTGAGGGTCGACAATTTTTCAATCAGATGGTGAGAACATATAATATTGAACCAACAATGGAACATTATGGATGCTTAGTAGATTTGCTTGGAAGAGCTGGTCTGGTTGAAGAAGCTTATGAAATTATCAAAAACATGCCAATGGAGCCTAATTCAGTTATACTACGGAGTTTCTTGGGTGCCTGTAGGAACCACAACATAGTTTTTACTTTAGATGATAACTTCAGGAAAGTCCTTACATCAGAGTCTCACCTTGGAGCAAACTATGTACTTGCAGCTAATGTATCTTCTGTCTCTGGTTGTTGGGGTGATGCTGCTGATATGCGAGTATCCATGAAACAGAAGGGTGTGATAAAGGTTCCTGGATGCAGTTGGGTGGACTCTGGAGGTGGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

452

Amino Acids

49.83

Weight (kDa)

6.75

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 165 - 213 7.1e-10 PPR repeat family
PPR PF01535 168 - 194 9.9e-06 PPR repeat
PPR_2 PF13041 267 - 314 3e-07 PPR repeat family
E_motif PF20431 402 - 446 5.7e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018016)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44710
prunus_persica Prupe.6G010900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0081411
rosa_laevigata RLG00000036932
rosa_multiflora Rmu_sc0007768.1_g000022
rosa_roxburghii Rroxscaffold_1G00002130
rosa_rugosa Rorug05G0476200
rosa_samantha Rh5BG552100 Rh5CG574700 Rh5DG561700
rosa_wichuraiana Rw5G049070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 898
AccB1I GGYRCC 2 cut(s) 898, 1136
AccI GTMKAC 2 cut(s) 643, 967
AccII CGCG 1 cut(s) 466
AciI CCGC 6 cut(s) 10, 47, 194, 333, 447, 466
AclWI GGATC 1 cut(s) 411
AcsI RAATTY 4 cut(s) 15, 106, 208, 799
AcuI CTGAAG 1 cut(s) 1165
AfaI GTAC 4 cut(s) 180, 637, 900, 1227
AfiI CCNNNNNNNGG 3 cut(s) 73, 604, 961
AgsI TTSAA 7 cut(s) 20, 147, 251, 781, 979, 1007, 1067
AjnI CCWGG 1 cut(s) 1318
Alw21I GWGCWC 2 cut(s) 140, 239
Alw26I GTCTC 3 cut(s) 118, 1209, 1255
AlwI GGATC 1 cut(s) 411
AoxI GGCC 2 cut(s) 327, 949
ApeKI GCWGC 4 cut(s) 274, 367, 1232, 1271
ApoI RAATTY 4 cut(s) 15, 106, 208, 799
Asp700I GAANNNNTTC 2 cut(s) 767, 856
Asp718I GGTACC 1 cut(s) 898
AspLEI GCGC 1 cut(s) 466
AsuHPI GGTGA 6 cut(s) 23, 308, 461, 1000, 1199, 1277
AsuNHI GCTAGC 1 cut(s) 583
BanI GGYRCC 2 cut(s) 898, 1136
BanII GRGCYC 2 cut(s) 140, 239
Bbv12I GWGCWC 2 cut(s) 140, 239
BbvI GCAGC 4 cut(s) 261, 354, 1244, 1258
BccI CCATC 3 cut(s) 340, 699, 979
BcgI CGANNNNNNTGC 4 cut(s) 192, 219, 226, 253
BciT130I CCWGG 1 cut(s) 1320
BciVI GTATCC 2 cut(s) 521, 1297
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 3 cut(s) 118, 1209, 1255
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 3 cut(s) 368, 937, 1141
BfuI GTATCC 2 cut(s) 521, 1297
BglI GCCNNNNNGGC 1 cut(s) 948
BisI GCNGC 5 cut(s) 48, 275, 368, 1233, 1272
BlsI GCNGC 5 cut(s) 49, 276, 369, 1234, 1273
Bme1390I CCNGG 1 cut(s) 1320
BmiI GGNNCC 6 cut(s) 243, 900, 1104, 1138, 1148, 1317
BmrFI CCNGG 1 cut(s) 1320
BmsI GCATC 4 cut(s) 354, 1019, 1258, 1313
BmtI GCTAGC 1 cut(s) 587
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 580
BsaBI GATNNNNATC 3 cut(s) 40, 146, 402
BsaJI CCNNGG 1 cut(s) 1210
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 604, 961
Bse1I ACTGG 1 cut(s) 900
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 3 cut(s) 40, 146, 402
BseBI CCWGG 1 cut(s) 1320
BseDI CCNNGG 1 cut(s) 1210
BseGI GGATG 5 cut(s) 351, 369, 691, 1034, 1328
BseJI GATNNNNATC 3 cut(s) 40, 146, 402
BseLI CCNNNNNNNGG 3 cut(s) 73, 604, 961
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 900
BseXI GCAGC 4 cut(s) 261, 354, 1244, 1258
BsgI GTGCAG 1 cut(s) 260
Bsh1236I CGCG 1 cut(s) 466
Bsh1285I CGRYCG 3 cut(s) 38, 78, 337
BshFI GGCC 2 cut(s) 329, 951
BshNI GGYRCC 2 cut(s) 898, 1136
BsiEI CGRYCG 3 cut(s) 38, 78, 337
BsiHKAI GWGCWC 2 cut(s) 140, 239
BsiSI CCGG 2 cut(s) 74, 525
BslI CCNNNNNNNGG 3 cut(s) 73, 604, 961
BsmAI GTCTC 3 cut(s) 118, 1209, 1255
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 2 cut(s) 329, 951
Bsp1286I GDGCHC 2 cut(s) 140, 239
Bsp143I GATC 4 cut(s) 35, 94, 403, 838
BspACI CCGC 6 cut(s) 10, 47, 194, 333, 447, 466
BspANI GGCC 2 cut(s) 329, 951
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 6 cut(s) 243, 900, 1104, 1138, 1148, 1317
BspMAI CTGCAG 1 cut(s) 372
BspOI GCTAGC 1 cut(s) 587
BspPI GGATC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 1045
BspT107I GGYRCC 2 cut(s) 898, 1136
BsrDI GCAATG 1 cut(s) 673
BsrI ACTGG 1 cut(s) 900
BssECI CCNNGG 1 cut(s) 1210
BssMI GATC 4 cut(s) 35, 94, 403, 838
BssT1I CCWWGG 1 cut(s) 1210
Bst2UI CCWGG 1 cut(s) 1320
Bst6I CTCTTC 1 cut(s) 1045
BstAPI GCANNNNNTGC 1 cut(s) 920
BstC8I GCNNGC 5 cut(s) 136, 331, 538, 585, 623
BstDEI CTNAG 6 cut(s) 130, 270, 744, 946, 956, 1033
BstENI CCTNNNNNAGG 1 cut(s) 959
BstF5I GGATG 5 cut(s) 351, 369, 691, 1034, 1328
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 4 cut(s) 38, 97, 406, 841
BstMAI GTCTC 3 cut(s) 118, 1209, 1255
BstMBI GATC 4 cut(s) 35, 94, 403, 838
BstMCI CGRYCG 3 cut(s) 38, 78, 337
BstMWI GCNNNNNNNGC 3 cut(s) 920, 948, 1102
BstNI CCWGG 1 cut(s) 1320
BstNSI RCATGY 2 cut(s) 758, 1096
BstSCI CCNGG 1 cut(s) 1318
BstSFI CTRYAG 3 cut(s) 368, 937, 1141
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 261, 354, 1244, 1258
BsuI GTATCC 2 cut(s) 521, 1297
BsuRI GGCC 2 cut(s) 329, 951
BtsCI GGATG 5 cut(s) 351, 369, 691, 1034, 1328
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 5 cut(s) 136, 331, 538, 585, 623
CfoI GCGC 1 cut(s) 466
Csp6I GTAC 4 cut(s) 179, 636, 899, 1226
CviAII CATG 7 cut(s) 204, 755, 842, 880, 910, 1093, 1291
CviQI GTAC 4 cut(s) 179, 636, 899, 1226
DdeI CTNAG 6 cut(s) 130, 270, 744, 946, 956, 1033
DpnI GATC 4 cut(s) 37, 96, 405, 840
DpnII GATC 4 cut(s) 35, 94, 403, 838
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
Ecl136II GAGCTC 2 cut(s) 138, 237
Eco130I CCWWGG 1 cut(s) 1210
Eco147I AGGCCT 2 cut(s) 329, 951
Eco24I GRGCYC 2 cut(s) 140, 239
Eco53kI GAGCTC 2 cut(s) 138, 237
Eco57I CTGAAG 1 cut(s) 1165
EcoICRI GAGCTC 2 cut(s) 138, 237
EcoNI CCTNNNNNAGG 1 cut(s) 959
EcoRI GAATTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 1318
EcoT14I CCWWGG 1 cut(s) 1210
EcoT38I GRGCYC 2 cut(s) 140, 239
ErhI CCWWGG 1 cut(s) 1210
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 7 cut(s) 207, 758, 845, 883, 913, 1096, 1294
FatI CATG 7 cut(s) 203, 754, 841, 879, 909, 1092, 1290
FauI CCCGC 1 cut(s) 17
FauNDI CATATG 1 cut(s) 774
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 643, 967
Fnu4HI GCNGC 5 cut(s) 48, 275, 368, 1233, 1272
FokI GGATG 5 cut(s) 358, 376, 678, 1041, 1335
FriOI GRGCYC 2 cut(s) 140, 239
Fsp4HI GCNGC 5 cut(s) 48, 275, 368, 1233, 1272
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 465
GluI GCNGC 5 cut(s) 48, 275, 368, 1233, 1272
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 2 cut(s) 329, 951
HapII CCGG 2 cut(s) 74, 525
HhaI GCGC 1 cut(s) 466
Hin1II CATG 7 cut(s) 207, 758, 845, 883, 913, 1096, 1294
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 968
HindII GTYRAC 1 cut(s) 968
HindIII AAGCTT 2 cut(s) 619, 1071
HinfI GANTC 7 cut(s) 251, 261, 398, 670, 857, 1202, 1337
HpaII CCGG 2 cut(s) 74, 525
HphI GGTGA 6 cut(s) 23, 308, 461, 1000, 1199, 1277
Hpy166II GTNNAC 4 cut(s) 636, 644, 968, 1336
Hpy188I TCNGA 8 cut(s) 118, 246, 260, 271, 403, 431, 984, 1201
Hpy188III TCNNGA 5 cut(s) 92, 572, 733, 1183, 1341
Hpy8I GTNNAC 4 cut(s) 636, 644, 968, 1336
HpyAV CCTTC 4 cut(s) 319, 463, 778, 1294
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 8 cut(s) 203, 230, 277, 370, 540, 805, 1232, 1326
HpyF10VI GCNNNNNNNGC 3 cut(s) 920, 948, 1102
HpyF3I CTNAG 6 cut(s) 130, 270, 744, 946, 956, 1033
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 7 cut(s) 207, 758, 845, 883, 913, 1096, 1294
HspAI GCGC 1 cut(s) 464
KpnI GGTACC 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 4 cut(s) 35, 94, 403, 838
LguI GCTCTTC 1 cut(s) 1045
LmnI GCTCC 3 cut(s) 69, 1102, 1214
Lsp1109I GCAGC 4 cut(s) 261, 354, 1244, 1258
LweI GCATC 4 cut(s) 354, 1019, 1258, 1313
MaeI CTAG 1 cut(s) 584
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 2 cut(s) 296, 607
MalI GATC 4 cut(s) 37, 96, 405, 840
MboI GATC 4 cut(s) 35, 94, 403, 838
MboII GAAGA 5 cut(s) 47, 256, 1062, 1079, 1236
MhlI GDGCHC 2 cut(s) 140, 239
MluCI AATT 9 cut(s) 15, 27, 106, 161, 208, 799, 971, 1080, 1108
MlyI GAGTC 2 cut(s) 1211, 1331
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 9 cut(s) 201, 347, 415, 445, 472, 555, 955, 962, 1337
MroXI GAANNNNTTC 2 cut(s) 767, 856
MseI TTAA 2 cut(s) 291, 873
MslI CAYNNNNRTG 2 cut(s) 846, 1097
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 2 cut(s) 74, 525
MspR9I CCNGG 1 cut(s) 1320
MvaI CCWGG 1 cut(s) 1320
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 3 cut(s) 920, 948, 1102
NdeI CATATG 1 cut(s) 774
NdeII GATC 4 cut(s) 35, 94, 403, 838
NheI GCTAGC 1 cut(s) 583
NlaIII CATG 7 cut(s) 207, 758, 845, 883, 913, 1096, 1294
NlaIV GGNNCC 6 cut(s) 243, 900, 1104, 1138, 1148, 1317
NmuCI GTSAC 2 cut(s) 296, 607
NspI RCATGY 2 cut(s) 758, 1096
PceI AGGCCT 2 cut(s) 329, 951
PciSI GCTCTTC 1 cut(s) 1045
PdmI GAANNNNTTC 2 cut(s) 767, 856
PfeI GAWTC 5 cut(s) 251, 261, 398, 670, 857
PfoI TCCNGGA 1 cut(s) 1318
PkrI GCNGC 5 cut(s) 49, 276, 369, 1234, 1273
Ple19I CGATCG 1 cut(s) 38
PleI GAGTC 2 cut(s) 1210, 1331
PpsI GAGTC 2 cut(s) 1210, 1331
Psp124BI GAGCTC 2 cut(s) 140, 239
Psp6I CCWGG 1 cut(s) 1318
PspGI CCWGG 1 cut(s) 1318
PspN4I GGNNCC 6 cut(s) 243, 900, 1104, 1138, 1148, 1317
PstI CTGCAG 1 cut(s) 372
PvuI CGATCG 1 cut(s) 38
RsaI GTAC 4 cut(s) 180, 637, 900, 1227
RsaNI GTAC 4 cut(s) 179, 636, 899, 1226
RseI CAYNNNNRTG 2 cut(s) 846, 1097
SacI GAGCTC 2 cut(s) 140, 239
SalI GTCGAC 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 1045
SaqAI TTAA 2 cut(s) 291, 873
SatI GCNGC 5 cut(s) 48, 275, 368, 1233, 1272
Sau3AI GATC 4 cut(s) 35, 94, 403, 838
SchI GAGTC 2 cut(s) 1211, 1331
ScrFI CCNGG 1 cut(s) 1320
SduI GDGCHC 2 cut(s) 140, 239
SfaNI GCATC 4 cut(s) 354, 1019, 1258, 1313
SfcI CTRYAG 3 cut(s) 368, 937, 1141
SmiMI CAYNNNNRTG 2 cut(s) 846, 1097
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 9 cut(s) 15, 27, 106, 161, 208, 799, 971, 1080, 1108
SseBI AGGCCT 2 cut(s) 329, 951
SsiI CCGC 6 cut(s) 10, 47, 194, 333, 447, 466
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 584
SstI GAGCTC 2 cut(s) 140, 239
StuI AGGCCT 2 cut(s) 329, 951
StyD4I CCNGG 1 cut(s) 1318
StyI CCWWGG 1 cut(s) 1210
TaiI ACGT 1 cut(s) 534
TaqII GACCGA 1 cut(s) 595
TasI AATT 9 cut(s) 15, 27, 106, 161, 208, 799, 971, 1080, 1108
TatI WGTACW 1 cut(s) 1225
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 5 cut(s) 251, 261, 398, 670, 857
Tru1I TTAA 2 cut(s) 291, 873
Tru9I TTAA 2 cut(s) 291, 873
TscAI CASTG 1 cut(s) 377
TseFI GTSAC 2 cut(s) 296, 607
TseI GCWGC 4 cut(s) 274, 367, 1232, 1271
Tsp45I GTSAC 2 cut(s) 296, 607
TspDTI ATGAA 6 cut(s) 398, 593, 606, 662, 1092, 1307
TspGWI ACGGA 1 cut(s) 1138
TspRI CASTG 1 cut(s) 377
XagI CCTNNNNNAGG 1 cut(s) 959
XapI RAATTY 4 cut(s) 15, 106, 208, 799
XceI RCATGY 2 cut(s) 758, 1096
XmiI GTMKAC 2 cut(s) 643, 967
XmnI GAANNNNTTC 2 cut(s) 767, 856
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.