FvH4_4g00020
HSP70 Family

hsp70-Hsp90 organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018828
Physical Location & Seq
Forward (+)
19354 .. 24549
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00020.t4

Sequence Viewer

Length: 1053 bp
ATGGATACGGAAAACGAATTCCTTGAAACTCTAGTGGATCATTTTCTAGCAAACATCCGTAGGACATATGTTCGTGCTCCAGAGCCAGAGAGCTTGAGTAGTGTTAGAAACCAGGCTAAAAAGTTGGAAGAGTATCTGAGAAGCAACAGTAATCATCCCACTGTTGCTGCCATGATTGAAAATGCTTTGTCGAAATTTGAATTTGAGGAAGATCAGTGGAATGGGAAAATGAGAATGGCTGATGAATTGCGGCAAAAAGGAAATGGGTGTATGCAGGGTAAAAAATTCAGAGACGCTGTGAGGGAATATACATATGCACTCGAGTGGAACCCCATGGACTCCAAGGCCTACTCCAGCAGATCGCTTGCTTTTTATATGTTGGGAATGGACATTGAAGCACTGGATGATGCAGAGAAATGCGTTGAGATCGATCCGAAGTTTTCAAGAGGGTATCTCCGGAAAGGGTCAGTTTTGTTGAGAAACAAAGAATTTGCAAAAGCTCTGGAAACCTTCCAAGAGGGTTTGGAATGTGATCCGACTGACCAGTACTTGAACCGAGGTGTGCGGAAGTGTTTGGAGCACACAGTGAAGAATGGCAACGGGGTAACTAGTGGGAACCAGGCTGGTGAGATAGTTAAGGGTGGAAGTAGGGTGGGTCAACAAAACGGATCAGCAATACAAGAGGTTGACATGCCTTGTGTTGAAACTGTTACTCCCAAGCCTGGAACTTTTGTATTGAAGCTGTCGAATGTGATCTTGACTGCTGAAGTTGTAGCGGTTATCACTGATAATCTGAGACTAAGGTTGCCGAGTCTGATCAGTTCCTTCATAGCAGTGTGCGGACACGTTGAGGTCTTGAGGCATCATCAGTTGGCCCAGAATAAGTCCACATTGATGAAACTTTTATGTGCAAGCTTGATGTTCATGTGTATTCTGATAGAGTATCTTGCTCATGTGCTGCATAAAAATGTTGGAAATCTGGAGAATTTTGGAAGGGAAGCCTGGCCGTTTCATGTTGCTGTGGTGATTGCTTCACTACTCCTCAACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05020 map05020
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.41

Weight (kDa)

6.18

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 67 - 148 1.4e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018828)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00020 FvH4_4g00020 FvH4_4g00020 FvH4_4g00020 FvH4_4g00020 FvH4_4g00020
rosa_chinensis RchiOBHm_Chr4g0388861
rosa_rugosa Rorug03G0320600
rosa_samantha Rh4BG018300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 456
AciI CCGC 4 cut(s) 250, 565, 776, 840
AclWI GGATC 4 cut(s) 45, 425, 527, 676
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 6 cut(s) 17, 194, 200, 284, 488, 985
AcuI CTGAAG 1 cut(s) 786
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 844
AgsI TTSAA 8 cut(s) 26, 179, 200, 395, 444, 553, 704, 739
AhlI ACTAGT 1 cut(s) 608
AjnI CCWGG 4 cut(s) 111, 618, 721, 1001
AleI CACNNNNGTG 1 cut(s) 322
AluBI AGCT 4 cut(s) 93, 500, 742, 915
AluI AGCT 4 cut(s) 93, 500, 742, 915
Alw21I GWGCWC 2 cut(s) 79, 582
Alw26I GTCTC 2 cut(s) 285, 790
AlwI GGATC 4 cut(s) 45, 425, 527, 676
Ama87I CYCGRG 1 cut(s) 320
Aor13HI TCCGGA 1 cut(s) 456
AoxI GGCC 3 cut(s) 345, 873, 1004
ApeKI GCWGC 2 cut(s) 167, 958
ApoI RAATTY 6 cut(s) 17, 194, 200, 284, 488, 985
AspS9I GGNCC 1 cut(s) 874
AsuHPI GGTGA 2 cut(s) 638, 1036
AvaI CYCGRG 1 cut(s) 320
Bbv12I GWGCWC 2 cut(s) 79, 582
BbvI GCAGC 2 cut(s) 154, 945
BceAI ACGGC 1 cut(s) 991
BciT130I CCWGG 4 cut(s) 113, 620, 723, 1003
BclI TGATCA 1 cut(s) 816
BcoDI GTCTC 2 cut(s) 285, 790
BcuI ACTAGT 1 cut(s) 608
BfaI CTAG 3 cut(s) 32, 47, 609
BisI GCNGC 3 cut(s) 168, 251, 959
BlsI GCNGC 3 cut(s) 169, 252, 960
BmcAI AGTACT 1 cut(s) 548
Bme1390I CCNGG 4 cut(s) 113, 620, 723, 1003
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 874
BmiI GGNNCC 2 cut(s) 329, 617
BmrFI CCNGG 4 cut(s) 113, 620, 723, 1003
BmsI GCATC 2 cut(s) 397, 871
BplI GAGNNNNNCTC 2 cut(s) 438, 470
BpmI CTGGAG 3 cut(s) 63, 337, 1001
BpuEI CTTGAG 2 cut(s) 115, 877
Bsa29I ATCGAT 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 333, 342, 556
BsaWI WCCGGW 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse1I ACTGG 2 cut(s) 405, 544
BseAI TCCGGA 1 cut(s) 456
BseBI CCWGG 4 cut(s) 113, 620, 723, 1003
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 333, 342, 556
BseGI GGATG 3 cut(s) 54, 154, 409
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 2 cut(s) 128, 785
BseNI ACTGG 2 cut(s) 405, 544
BseRI GAGGAG 1 cut(s) 1031
BseXI GCAGC 2 cut(s) 154, 945
BshFI GGCC 3 cut(s) 347, 875, 1006
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 79, 582
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 1 cut(s) 457
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 2 cut(s) 285, 790
BsmBI CGTCTC 1 cut(s) 285
BsnI GGCC 3 cut(s) 347, 875, 1006
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 79, 582
Bsp13I TCCGGA 1 cut(s) 456
Bsp143I GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 250, 565, 776, 840
BspANI GGCC 3 cut(s) 347, 875, 1006
BspCNI CTCAG 2 cut(s) 129, 786
BspDI ATCGAT 1 cut(s) 429
BspEI TCCGGA 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 329, 617
BspPI GGATC 4 cut(s) 45, 425, 527, 676
BsrI ACTGG 2 cut(s) 405, 544
BssECI CCNNGG 3 cut(s) 333, 342, 556
BssMI GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
BssT1I CCWWGG 2 cut(s) 333, 342
Bst2UI CCWGG 4 cut(s) 113, 620, 723, 1003
Bst4CI ACNGT 4 cut(s) 149, 163, 586, 709
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 366, 913
BstDEI CTNAG 3 cut(s) 137, 794, 800
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 54, 154, 409
BstKTI GATC 9 cut(s) 40, 214, 362, 429, 433, 535, 671, 756, 819
BstMAI GTCTC 2 cut(s) 285, 790
BstMBI GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
BstNI CCWGG 4 cut(s) 113, 620, 723, 1003
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 4 cut(s) 111, 618, 721, 1001
BstV1I GCAGC 2 cut(s) 154, 945
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 3 cut(s) 347, 875, 1006
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 3 cut(s) 54, 154, 409
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 6 cut(s) 159, 221, 398, 591, 783, 840
Cac8I GCNNGC 2 cut(s) 366, 913
Cfr13I GGNCC 1 cut(s) 874
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 547
CviAII CATG 6 cut(s) 172, 334, 691, 925, 953, 1013
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 3 cut(s) 137, 794, 800
DpnI GATC 9 cut(s) 39, 213, 361, 428, 432, 534, 670, 755, 818
DpnII GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
DraIII CACNNNGTG 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 1004
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 2 cut(s) 333, 342
Eco147I AGGCCT 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 4 cut(s) 111, 618, 721, 1001
EcoT14I CCWWGG 2 cut(s) 333, 342
ErhI CCWWGG 2 cut(s) 333, 342
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 6 cut(s) 175, 337, 694, 928, 956, 1016
FatI CATG 6 cut(s) 171, 333, 690, 924, 952, 1012
FauNDI CATATG 2 cut(s) 67, 313
FbaI TGATCA 1 cut(s) 816
Fnu4HI GCNGC 3 cut(s) 168, 251, 959
FokI GGATG 3 cut(s) 41, 141, 416
Fsp4HI GCNGC 3 cut(s) 168, 251, 959
FspBI CTAG 3 cut(s) 32, 47, 609
GluI GCNGC 3 cut(s) 168, 251, 959
GsuI CTGGAG 3 cut(s) 63, 337, 1001
HaeIII GGCC 3 cut(s) 347, 875, 1006
HapII CCGG 1 cut(s) 457
HgaI GACGC 1 cut(s) 302
Hin1II CATG 6 cut(s) 175, 337, 694, 928, 956, 1016
HincII GTYRAC 2 cut(s) 659, 688
HindII GTYRAC 2 cut(s) 659, 688
HindIII AAGCTT 1 cut(s) 913
HinfI GANTC 2 cut(s) 338, 811
HpaII CCGG 1 cut(s) 457
HphI GGTGA 2 cut(s) 638, 1036
Hpy166II GTNNAC 3 cut(s) 659, 688, 888
Hpy188I TCNGA 7 cut(s) 138, 290, 435, 537, 795, 816, 936
Hpy188III TCNNGA 7 cut(s) 80, 444, 457, 503, 757, 856, 980
Hpy8I GTNNAC 3 cut(s) 659, 688, 888
HpyAV CCTTC 3 cut(s) 520, 835, 987
HpyCH4III ACNGT 4 cut(s) 149, 163, 586, 709
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 6 cut(s) 274, 317, 410, 494, 911, 961
HpyF3I CTNAG 3 cut(s) 137, 794, 800
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 6 cut(s) 175, 337, 694, 928, 956, 1016
Kpn2I TCCGGA 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
LmnI GCTCC 2 cut(s) 82, 577
Lsp1109I GCAGC 2 cut(s) 154, 945
LweI GCATC 2 cut(s) 397, 871
MaeI CTAG 3 cut(s) 32, 47, 609
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 2 cut(s) 604, 709
MalI GATC 9 cut(s) 39, 213, 361, 428, 432, 534, 670, 755, 818
MboI GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
MboII GAAGA 3 cut(s) 140, 221, 601
MhlI GDGCHC 2 cut(s) 79, 582
MluCI AATT 7 cut(s) 17, 194, 200, 245, 284, 488, 985
MlyI GAGTC 2 cut(s) 332, 820
MmeI TCCRAC 3 cut(s) 105, 560, 952
MnlI CCTC 9 cut(s) 199, 294, 440, 511, 551, 676, 844, 852, 1052
MroI TCCGGA 1 cut(s) 456
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 4 cut(s) 322, 833, 893, 966
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 4 cut(s) 113, 620, 723, 1003
MvaI CCWGG 4 cut(s) 113, 620, 723, 1003
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 2 cut(s) 67, 313
NdeII GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
NlaIII CATG 6 cut(s) 175, 337, 694, 928, 956, 1016
NlaIV GGNNCC 2 cut(s) 329, 617
NmeAIII GCCGAG 1 cut(s) 834
NspI RCATGY 1 cut(s) 694
OliI CACNNNNGTG 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 320
PceI AGGCCT 1 cut(s) 347
PkrI GCNGC 3 cut(s) 169, 252, 960
PleI GAGTC 2 cut(s) 332, 819
PpsI GAGTC 2 cut(s) 332, 819
Psp6I CCWGG 4 cut(s) 111, 618, 721, 1001
PspGI CCWGG 4 cut(s) 111, 618, 721, 1001
PspN4I GGNNCC 2 cut(s) 329, 617
PspPI GGNCC 1 cut(s) 874
PspXI VCTCGAGB 1 cut(s) 320
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 4 cut(s) 322, 833, 893, 966
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 3 cut(s) 168, 251, 959
Sau3AI GATC 9 cut(s) 37, 211, 359, 426, 430, 532, 668, 753, 816
Sau96I GGNCC 1 cut(s) 874
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 2 cut(s) 332, 820
ScrFI CCNGG 4 cut(s) 113, 620, 723, 1003
SduI GDGCHC 2 cut(s) 79, 582
SfaNI GCATC 2 cut(s) 397, 871
Sfr274I CTCGAG 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 4 cut(s) 322, 833, 893, 966
SmlI CTYRAG 3 cut(s) 94, 320, 856
SmoI CTYRAG 3 cut(s) 94, 320, 856
SpeI ACTAGT 1 cut(s) 608
Sse9I AATT 7 cut(s) 17, 194, 200, 245, 284, 488, 985
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 4 cut(s) 250, 565, 776, 840
SspMI CTAG 3 cut(s) 32, 47, 609
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 4 cut(s) 111, 618, 721, 1001
StyI CCWWGG 2 cut(s) 333, 342
TaaI ACNGT 4 cut(s) 149, 163, 586, 709
TaiI ACGT 1 cut(s) 849
TaqI TCGA 4 cut(s) 191, 321, 429, 746
TasI AATT 7 cut(s) 17, 194, 200, 245, 284, 488, 985
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 6 cut(s) 166, 221, 405, 591, 790, 840
TseI GCWGC 2 cut(s) 167, 958
TspDTI ATGAA 5 cut(s) 258, 817, 911, 913, 1001
TspGWI ACGGA 3 cut(s) 23, 47, 681
TspRI CASTG 6 cut(s) 166, 221, 405, 591, 790, 840
XapI RAATTY 6 cut(s) 17, 194, 200, 284, 488, 985
XceI RCATGY 1 cut(s) 694
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 3 cut(s) 32, 47, 609
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.