Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0008462

Physical Location & Seq

Genomic Coordinates Reverse (-)

2066483 .. 2077203

Gene Structure 10721 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g02250.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGCCTTCTCTGAGGCGACCCACTTCAGCTTCAATTCTCGTATCTCTGCTGTTCCTATTCCTCTCTGTCTTTCTCTGCCCAACTCTCGCCGACGGCGTCACTCCCGAGGAGGCCAAGCAGCTCAGGGACGAGGTCAGGGAAATGTTTTATCATGCATTTAATGGGTACATGGACCATGCTTTTCCACTCGATGAGTTAAAGCCTCAGTCTTGTGGGGGTGAAGATAGCCTCGGTGGATACGCGCTAACTTTGATTGACGCTTTAGACACACTAGCTTTACTTGGTGACCGGGACCGCTTTTCTGCCTCTGTTGAATGGATCAGTAAAAACCTTCAGTTTGATATAAATAAAACAGTATCTGTCTTTGAGACTACCATCCGGGTCCTTGGGGGTTTGCTCTCTGCACACCTTATAGCAAGTGATTATGCTACGGGCATGAGAATTCCAACGTATGACAACCAGTTACTGAACTTAGCTGAGGATCTAGGCCGACGATTGTTACCTGCATTTGACACTCCTTCAGGAATTCCTTTTGGATCTGTCAATCTGAGGCATGGAGTTGATGAACATGAAAGCAAGATTACATCAACAGCTGGTGGTGGGACTTTGACTTTGGAATTTGGAGTGCTCAGCCGCTTGACAAATGATTCCATATTTGAACAAGCGACCAAGAATGCAGTGCGTGGACTTTGGGCTCAGCGTTCAAAGCTCAATTTAGTTGGTGCCCATATTAATGTTTTTACAGGTGAATGGACACAAAAGGATGCTGGAATAGGAACCAGTATTGATTCTTTTTATGAGTATCTTCTAAAGGCTTATCTGTTATTTGGAGATGAAGAATATCTGTACATATTTCAAGAAGCTTATGCTGCCGCTATGCAATATCTTTACAATGATCCTTGGTATGTAGAGGTAAATATGGATTCTGCTGCTGTTGTGTGGCCATTGTTTAACAGTTTACAAGCATTTTGGCCAGGACTTCAGGTTTTAGCTGGAGATATTGATCCTGCAATTCGAACTCATACTGCCTTCTTTAGTGTGTGGAAAAAATATGGTTTCACTCCGGAAGGTTTCAATCTTGCTACATTCAGTGTTCAGCATGGACAAAGAAGTTATCCGCTGCGTCCAGAGTTAATGGAGAGCACATATTGGCTCTATAAAGCTACTAGAGATCCCAAGTATCTTGATGCAGGACGTGACATGGTTGCCAGTTTGCAGTACAGTGCACGATGCGCCTGCGGATATTGTCATATCGCAGATGTCGAGTCCCATAAGCAGGAAGATCATATGGAGAGTTTTTTTCTAGCGGAAACAGTAAAATATTTGTGGCTACTCTTTGATTTGGCCGTGGGACCTGATAACCTTGTTGATAATGGACCATACAAGTACATCTTTAGTACTGAAGGCCACCTACTGCCTGCAACCCCTGAAATATCTTTAGTGCAGGAACATTGCTCGTATTTTGGGGCTTATTGTAATACTAGCAACATTAGACAGGATGTATTGGATGTTGCAACTAATCCTCGAGATAACTTATATGGAAGGGTTGGCACTGCGTTACCATCCAACTCCCGTTATTCTGATTCTACTCCCGTATCAGGATTTATAAAAGGGATTTGCCCAGGACTAACTCATGGGCAAAAATTTGGTATATCATATATATCACCAGGCCACAGAGCTCATGAGGATGACTCCAGGAACCAAGGAGAACAAACTAATGGTGAGAGCCATTCAATAGTGATACTGCAAAGCCAAAATTCTGATTACTCACCATCAGAGAATAAGTACCATGAAAAGTTGGTGGAACCTCACGAGAGAAAACAAACAAGTGATCCACCTCAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004571 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006491 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015923 GO:0015924 GO:0016787 GO:0016798 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0033554 GO:0034976 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0071704 GO:1901135 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Glyco_hydro_47

Protein Analysis

616

Amino Acids

68.78

Weight (kDa)

5.08

Isoelectric Point (pI)

40.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_47	PF01532	1 - 426	6.2e-120	Glycosyl hydrolase family 47

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43710 AT5G43710 AT5G43710
fragaria_vesca	FvH4_4g02250 FvH4_4g02250 FvH4_4g02250 FvH4_4g02250 FvH4_4g02250
malus_domestica	MD16G1206300.v1.1
prunus_persica	Prupe.1G027300_v2.0.a1 Prupe.1G027300_v2.0.a1 Prupe.1G027300_v2.0.a1
pyrus_communis	pycom16g17350
rosa_chinensis	RchiOBHm_Chr4g0389601
rosa_laevigata	RLG00000009969
rosa_multiflora	Rmu_ssc0000478.1_g000010
rosa_roxburghii	Rroxscaffold_5G00335560
rosa_rugosa	Rorug03G0324100
rosa_samantha	Rh4AG028600 Rh4BG021900 Rh4CG030800 Rh4DG022400
rosa_wichuraiana	Rw4G002140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1607
Acc36I	ACCTGC	1 cut(s)	511
AccB1I	GGYRCC	1 cut(s)	722
AccII	CGCG	1 cut(s)	242
AccIII	TCCGGA	1 cut(s)	1063
AciI	CCGC	6 cut(s)	295, 634, 873, 1118, 1239, 1307
AclWI	GGATC	7 cut(s)	326, 489, 544, 890, 998, 1166, 1826
AcoI	YGGCCR	3 cut(s)	941, 971, 1344
AcsI	RAATTY	5 cut(s)	441, 525, 617, 1643, 1756
AcuI	CTGAAG	5 cut(s)	9, 317, 504, 965, 1422
AcyI	GRCGYC	1 cut(s)	96
AfaI	GTAC	6 cut(s)	167, 848, 1220, 1388, 1399, 1787
AfiI	CCNNNNNNNGG	2 cut(s)	1276, 1598
AgsI	TTSAA	7 cut(s)	33, 314, 659, 705, 857, 1075, 1734
AjiI	CACGTC	1 cut(s)	1196
AjnI	CCWGG	4 cut(s)	973, 1621, 1666, 1694
Alw21I	GWGCWC	4 cut(s)	630, 1145, 1228, 1681
Alw26I	GTCTC	1 cut(s)	362
Alw44I	GTGCAC	1 cut(s)	1224
AlwI	GGATC	7 cut(s)	326, 489, 544, 890, 998, 1166, 1826
AlwNI	CAGNNNCTG	2 cut(s)	359, 466
Ama87I	CYCGRG	2 cut(s)	104, 1524
Aor13HI	TCCGGA	1 cut(s)	1063
AoxI	GGCC	7 cut(s)	111, 487, 941, 971, 1344, 1405, 1669
ApaLI	GTGCAC	1 cut(s)	1224
ApeKI	GCWGC	4 cut(s)	118, 869, 929, 1120
ApoI	RAATTY	5 cut(s)	441, 525, 617, 1643, 1756
ArsI	GACNNNNNNTTYG	2 cut(s)	595, 627
AseI	ATTAAT	1 cut(s)	732
AspLEI	GCGC	2 cut(s)	244, 1235
AspS9I	GGNCC	5 cut(s)	172, 292, 382, 1352, 1376
AsuC2I	CCSGG	2 cut(s)	290, 380
AsuHPI	GGTGA	6 cut(s)	230, 296, 758, 1656, 1733, 1761
AsuII	TTCGAA	1 cut(s)	1015
AvaI	CYCGRG	2 cut(s)	104, 1524
AvaII	GGWCC	5 cut(s)	172, 292, 382, 1352, 1376
BaeGI	GKGCMC	2 cut(s)	727, 1228
BaeI	ACNNNNGTAYC	2 cut(s)	1578, 1611
BalI	TGGCCA	2 cut(s)	943, 973
BanI	GGYRCC	1 cut(s)	722
BanII	GRGCYC	2 cut(s)	697, 1681
BarI	GAAGNNNNNNTAC	2 cut(s)	1395, 1427
BauI	CACGAG	1 cut(s)	1811
Bbv12I	GWGCWC	4 cut(s)	630, 1145, 1228, 1681
BbvCI	CCTCAGC	1 cut(s)	477
BbvI	GCAGC	4 cut(s)	130, 856, 916, 1107
BccI	CCATC	3 cut(s)	383, 1570, 1780
BceAI	ACGGC	2 cut(s)	109, 1331
BcgI	CGANNNNNNTGC	2 cut(s)	1218, 1252
BciT130I	CCWGG	4 cut(s)	975, 1623, 1668, 1696
BciVI	GTATCC	1 cut(s)	230
BcnI	CCSGG	2 cut(s)	290, 380
BcoDI	GTCTC	1 cut(s)	362
BfaI	CTAG	5 cut(s)	272, 485, 1167, 1304, 1482
BfuAI	ACCTGC	1 cut(s)	511
BfuI	GTATCC	1 cut(s)	230
BisI	GCNGC	6 cut(s)	119, 634, 870, 873, 930, 1121
BlpI	GCTNAGC	2 cut(s)	629, 696
BlsI	GCNGC	6 cut(s)	120, 635, 871, 874, 931, 1122
BmcAI	AGTACT	1 cut(s)	1399
Bme1390I	CCNGG	6 cut(s)	290, 380, 975, 1623, 1668, 1696
Bme18I	GGWCC	5 cut(s)	172, 292, 382, 1352, 1376
BmeT110I	CYCGRG	2 cut(s)	104, 1524
BmgBI	CACGTC	1 cut(s)	1196
BmgT120I	GGNCC	5 cut(s)	172, 292, 382, 1352, 1376
BmiI	GGNNCC	7 cut(s)	293, 383, 724, 778, 1353, 1700, 1806
BmrFI	CCNGG	6 cut(s)	290, 380, 975, 1623, 1668, 1696
BmsI	GCATC	3 cut(s)	754, 1177, 1220
BplI	GAGNNNNNCTC	2 cut(s)	1676, 1708
BpmI	CTGGAG	2 cut(s)	1014, 1678
Bpu10I	CCTNAGC	2 cut(s)	122, 477
Bpu1102I	GCTNAGC	2 cut(s)	629, 696
Bpu14I	TTCGAA	1 cut(s)	1015
BpuMI	CCSGG	2 cut(s)	290, 380
BsaBI	GATNNNNATC	1 cut(s)	1002
BsaHI	GRCGYC	1 cut(s)	96
BsaJI	CCNNGG	7 cut(s)	105, 229, 385, 899, 1347, 1621, 1702
BsaWI	WCCGGW	1 cut(s)	1063
Bsc4I	CCNNNNNNNGG	2 cut(s)	1276, 1598
Bse1I	ACTGG	3 cut(s)	460, 780, 1209
Bse3DI	GCAATG	1 cut(s)	1450
Bse8I	GATNNNNATC	1 cut(s)	1002
BseAI	TCCGGA	1 cut(s)	1063
BseBI	CCWGG	4 cut(s)	975, 1623, 1668, 1696
BseDI	CCNNGG	7 cut(s)	105, 229, 385, 899, 1347, 1621, 1702
BseGI	GGATG	6 cut(s)	375, 769, 1504, 1513, 1562, 1693
BseJI	GATNNNNATC	1 cut(s)	1002
BseLI	CCNNNNNNNGG	2 cut(s)	1276, 1598
BseMI	GCAATG	1 cut(s)	1450
BseMII	CTCAG	6 cut(s)	136, 218, 468, 539, 643, 710
BseNI	ACTGG	3 cut(s)	460, 780, 1209
BseRI	GAGGAG	1 cut(s)	122
BseSI	GKGCMC	2 cut(s)	727, 1228
BseXI	GCAGC	4 cut(s)	130, 856, 916, 1107
BsgI	GTGCAG	2 cut(s)	387, 1463
Bsh1236I	CGCG	1 cut(s)	242
BshFI	GGCC	7 cut(s)	113, 489, 943, 973, 1346, 1407, 1671
BshNI	GGYRCC	1 cut(s)	722
BsiHKAI	GWGCWC	4 cut(s)	630, 1145, 1228, 1681
BsiHKCI	CYCGRG	2 cut(s)	104, 1524
BsiSI	CCGG	3 cut(s)	289, 379, 1064
BslFI	GGGAC	5 cut(s)	140, 305, 616, 1252, 1365
BslI	CCNNNNNNNGG	2 cut(s)	1276, 1598
BsmAI	GTCTC	1 cut(s)	362
BsmFI	GGGAC	5 cut(s)	140, 305, 616, 1252, 1365
BsmI	GAATGC	1 cut(s)	679
BsnI	GGCC	7 cut(s)	113, 489, 943, 973, 1346, 1407, 1671
BsoBI	CYCGRG	2 cut(s)	104, 1524
Bsp119I	TTCGAA	1 cut(s)	1015
Bsp1286I	GDGCHC	6 cut(s)	630, 697, 727, 1145, 1228, 1681
Bsp13I	TCCGGA	1 cut(s)	1063
Bsp1407I	TGTACA	1 cut(s)	846
Bsp143I	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
Bsp1720I	GCTNAGC	2 cut(s)	629, 696
BspACI	CCGC	6 cut(s)	295, 634, 873, 1118, 1239, 1307
BspANI	GGCC	7 cut(s)	113, 489, 943, 973, 1346, 1407, 1671
BspCNI	CTCAG	6 cut(s)	135, 217, 469, 540, 642, 709
BspEI	TCCGGA	1 cut(s)	1063
BspFNI	CGCG	1 cut(s)	242
BspHI	TCATGA	1 cut(s)	1681
BspLI	GGNNCC	7 cut(s)	293, 383, 724, 778, 1353, 1700, 1806
BspMI	ACCTGC	1 cut(s)	511
BspPI	GGATC	7 cut(s)	326, 489, 544, 890, 998, 1166, 1826
BspT104I	TTCGAA	1 cut(s)	1015
BspT107I	GGYRCC	1 cut(s)	722
BsrDI	GCAATG	1 cut(s)	1450
BsrGI	TGTACA	1 cut(s)	846
BsrI	ACTGG	3 cut(s)	460, 780, 1209
BssECI	CCNNGG	7 cut(s)	105, 229, 385, 899, 1347, 1621, 1702
BssMI	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
BssNI	GRCGYC	1 cut(s)	96
BssSI	CACGAG	1 cut(s)	1811
BssT1I	CCWWGG	3 cut(s)	385, 899, 1702
Bst2BI	CACGAG	1 cut(s)	1811
Bst2UI	CCWGG	4 cut(s)	975, 1623, 1668, 1696
Bst4CI	ACNGT	4 cut(s)	355, 956, 1223, 1315
BstACI	GRCGYC	1 cut(s)	96
BstAUI	TGTACA	1 cut(s)	846
BstBI	TTCGAA	1 cut(s)	1015
BstC8I	GCNNGC	2 cut(s)	1237, 1419
BstDEI	CTNAG	9 cut(s)	11, 122, 204, 472, 477, 548, 629, 696, 1839
BstDSI	CCRYGG	1 cut(s)	1347
BstEII	GGTNACC	1 cut(s)	284
BstF5I	GGATG	6 cut(s)	375, 769, 1504, 1513, 1562, 1693
BstFNI	CGCG	1 cut(s)	242
BstHHI	GCGC	2 cut(s)	244, 1235
BstKTI	GATC	8 cut(s)	321, 484, 539, 898, 1006, 1174, 1285, 1834
BstMAI	GTCTC	1 cut(s)	362
BstMBI	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
BstMWI	GCNNNNNNNGC	3 cut(s)	706, 869, 1232
BstNI	CCWGG	4 cut(s)	975, 1623, 1668, 1696
BstPI	GGTNACC	1 cut(s)	284
BstSCI	CCNGG	6 cut(s)	288, 378, 973, 1621, 1666, 1694
BstSLI	GKGCMC	2 cut(s)	727, 1228
BstUI	CGCG	1 cut(s)	242
BstV1I	GCAGC	4 cut(s)	130, 856, 916, 1107
BstX2I	RGATCY	3 cut(s)	481, 536, 1171
BstYI	RGATCY	3 cut(s)	481, 536, 1171
BsuI	GTATCC	1 cut(s)	230
BsuRI	GGCC	7 cut(s)	113, 489, 943, 973, 1346, 1407, 1671
BtgI	CCRYGG	1 cut(s)	1347
BtrI	CACGTC	1 cut(s)	1196
BtsCI	GGATG	6 cut(s)	375, 769, 1504, 1513, 1562, 1693
BtsI	GCAGTG	2 cut(s)	684, 1551
BtsIMutI	CAGTG	4 cut(s)	684, 1096, 1228, 1551
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	2 cut(s)	1237, 1419
CaiI	CAGNNNCTG	2 cut(s)	359, 466
CciI	TCATGA	1 cut(s)	1681
CfoI	GCGC	2 cut(s)	244, 1235
Cfr13I	GGNCC	5 cut(s)	172, 292, 382, 1352, 1376
CseI	GACGC	3 cut(s)	85, 266, 1112
Csp6I	GTAC	6 cut(s)	166, 847, 1219, 1387, 1398, 1786
CviQI	GTAC	6 cut(s)	166, 847, 1219, 1387, 1398, 1786
DdeI	CTNAG	9 cut(s)	11, 122, 204, 472, 477, 548, 629, 696, 1839
DpnI	GATC	8 cut(s)	320, 483, 538, 897, 1005, 1173, 1284, 1833
DpnII	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
EaeI	YGGCCR	3 cut(s)	941, 971, 1344
Ecl136II	GAGCTC	1 cut(s)	1679
Eco130I	CCWWGG	3 cut(s)	385, 899, 1702
Eco24I	GRGCYC	2 cut(s)	697, 1681
Eco47I	GGWCC	5 cut(s)	172, 292, 382, 1352, 1376
Eco53kI	GAGCTC	1 cut(s)	1679
Eco57I	CTGAAG	5 cut(s)	9, 317, 504, 965, 1422
Eco88I	CYCGRG	2 cut(s)	104, 1524
Eco91I	GGTNACC	1 cut(s)	284
EcoICRI	GAGCTC	1 cut(s)	1679
EcoO109I	RGGNCCY	2 cut(s)	382, 1352
EcoO65I	GGTNACC	1 cut(s)	284
EcoRI	GAATTC	2 cut(s)	441, 525
EcoRII	CCWGG	4 cut(s)	973, 1621, 1666, 1694
EcoT14I	CCWWGG	3 cut(s)	385, 899, 1702
EcoT22I	ATGCAT	1 cut(s)	157
EcoT38I	GRGCYC	2 cut(s)	697, 1681
ErhI	CCWWGG	3 cut(s)	385, 899, 1702
FalI	AAGNNNNNCTT	2 cut(s)	1376, 1408
FaqI	GGGAC	5 cut(s)	140, 305, 616, 1252, 1365
FauNDI	CATATG	1 cut(s)	1287
Fnu4HI	GCNGC	6 cut(s)	119, 634, 870, 873, 930, 1121
FokI	GGATG	6 cut(s)	362, 776, 1511, 1520, 1549, 1700
FriOI	GRGCYC	2 cut(s)	697, 1681
Fsp4HI	GCNGC	6 cut(s)	119, 634, 870, 873, 930, 1121
FspBI	CTAG	5 cut(s)	272, 485, 1167, 1304, 1482
GlaI	GCGC	2 cut(s)	243, 1234
GluI	GCNGC	6 cut(s)	119, 634, 870, 873, 930, 1121
GsuI	CTGGAG	2 cut(s)	1014, 1678
HaeIII	GGCC	7 cut(s)	113, 489, 943, 973, 1346, 1407, 1671
HapII	CCGG	3 cut(s)	289, 379, 1064
HgaI	GACGC	3 cut(s)	85, 266, 1112
HhaI	GCGC	2 cut(s)	244, 1235
Hin1I	GRCGYC	1 cut(s)	96
Hin6I	GCGC	2 cut(s)	242, 1233
HinP1I	GCGC	2 cut(s)	242, 1233
HindIII	AAGCTT	1 cut(s)	861
HinfI	GANTC	6 cut(s)	647, 788, 923, 1265, 1583, 1691
HpaII	CCGG	3 cut(s)	289, 379, 1064
HphI	GGTGA	6 cut(s)	230, 296, 758, 1656, 1733, 1761
Hpy166II	GTNNAC	3 cut(s)	686, 959, 1226
Hpy188I	TCNGA	6 cut(s)	12, 549, 1582, 1762, 1777, 1842
Hpy8I	GTNNAC	3 cut(s)	686, 959, 1226
Hpy99I	CGWCG	2 cut(s)	95, 495
HpyAV	CCTTC	7 cut(s)	15, 341, 528, 1039, 1061, 1397, 1536
HpyCH4III	ACNGT	4 cut(s)	355, 956, 1223, 1315
HpyCH4IV	ACGT	2 cut(s)	449, 1195
HpyF10VI	GCNNNNNNNGC	3 cut(s)	706, 869, 1232
HpyF3I	CTNAG	9 cut(s)	11, 122, 204, 472, 477, 548, 629, 696, 1839
HpySE526I	ACGT	2 cut(s)	449, 1195
Hsp92I	GRCGYC	1 cut(s)	96
HspAI	GCGC	2 cut(s)	242, 1233
Kpn2I	TCCGGA	1 cut(s)	1063
Kzo9I	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
Lsp1109I	GCAGC	4 cut(s)	130, 856, 916, 1107
LweI	GCATC	3 cut(s)	754, 1177, 1220
MaeI	CTAG	5 cut(s)	272, 485, 1167, 1304, 1482
MaeII	ACGT	2 cut(s)	449, 1195
MaeIII	GTNAC	6 cut(s)	97, 284, 462, 498, 1196, 1557
MalI	GATC	8 cut(s)	320, 483, 538, 897, 1005, 1173, 1284, 1833
MboI	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
MboII	GAAGA	4 cut(s)	233, 797, 848, 1292
MflI	RGATCY	3 cut(s)	481, 536, 1171
MhlI	GDGCHC	6 cut(s)	630, 697, 727, 1145, 1228, 1681
MlsI	TGGCCA	2 cut(s)	943, 973
MluCI	AATT	8 cut(s)	33, 441, 525, 617, 712, 1011, 1643, 1756
MluNI	TGGCCA	2 cut(s)	943, 973
MlyI	GAGTC	2 cut(s)	1274, 1685
MmeI	TCCRAC	2 cut(s)	470, 1590
Mox20I	TGGCCA	2 cut(s)	943, 973
Mph1103I	ATGCAT	1 cut(s)	157
MroI	TCCGGA	1 cut(s)	1063
MscI	TGGCCA	2 cut(s)	943, 973
MseI	TTAA	5 cut(s)	159, 197, 732, 951, 1133
MslI	CAYNNNNRTG	2 cut(s)	732, 1686
Msp20I	TGGCCA	2 cut(s)	943, 973
MspA1I	CMGCKG	2 cut(s)	593, 1120
MspI	CCGG	3 cut(s)	289, 379, 1064
MspR9I	CCNGG	6 cut(s)	290, 380, 975, 1623, 1668, 1696
Mva1269I	GAATGC	1 cut(s)	679
MvaI	CCWGG	4 cut(s)	975, 1623, 1668, 1696
MvnI	CGCG	1 cut(s)	242
MwoI	GCNNNNNNNGC	3 cut(s)	706, 869, 1232
NciI	CCSGG	2 cut(s)	290, 380
NdeI	CATATG	1 cut(s)	1287
NdeII	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
NlaIV	GGNNCC	7 cut(s)	293, 383, 724, 778, 1353, 1700, 1806
NmuCI	GTSAC	3 cut(s)	97, 284, 1196
NsiI	ATGCAT	1 cut(s)	157
NspV	TTCGAA	1 cut(s)	1015
PaeR7I	CTCGAG	1 cut(s)	1524
PagI	TCATGA	1 cut(s)	1681
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 1260
PctI	GAATGC	1 cut(s)	679
PfeI	GAWTC	4 cut(s)	647, 788, 923, 1583
PflFI	GACNNNGTC	2 cut(s)	95, 131
PfoI	TCCNGGA	1 cut(s)	1694
PkrI	GCNGC	6 cut(s)	120, 635, 871, 874, 931, 1122
PleI	GAGTC	2 cut(s)	1273, 1685
PpsI	GAGTC	2 cut(s)	1273, 1685
PpuMI	RGGWCCY	2 cut(s)	382, 1352
PshBI	ATTAAT	1 cut(s)	732
PsiI	TTATAA	1 cut(s)	1607
Psp124BI	GAGCTC	1 cut(s)	1681
Psp5II	RGGWCCY	2 cut(s)	382, 1352
Psp6I	CCWGG	4 cut(s)	973, 1621, 1666, 1694
PspEI	GGTNACC	1 cut(s)	284
PspGI	CCWGG	4 cut(s)	973, 1621, 1666, 1694
PspN4I	GGNNCC	7 cut(s)	293, 383, 724, 778, 1353, 1700, 1806
PspPI	GGNCC	5 cut(s)	172, 292, 382, 1352, 1376
PspPPI	RGGWCCY	2 cut(s)	382, 1352
PstNI	CAGNNNCTG	2 cut(s)	359, 466
PsuI	RGATCY	3 cut(s)	481, 536, 1171
PsyI	GACNNNGTC	2 cut(s)	95, 131
PvuII	CAGCTG	1 cut(s)	593
RsaI	GTAC	6 cut(s)	167, 848, 1220, 1388, 1399, 1787
RsaNI	GTAC	6 cut(s)	166, 847, 1219, 1387, 1398, 1786
RseI	CAYNNNNRTG	2 cut(s)	732, 1686
SacI	GAGCTC	1 cut(s)	1681
SaqAI	TTAA	5 cut(s)	159, 197, 732, 951, 1133
SatI	GCNGC	6 cut(s)	119, 634, 870, 873, 930, 1121
Sau3AI	GATC	8 cut(s)	318, 481, 536, 895, 1003, 1171, 1282, 1831
Sau96I	GGNCC	5 cut(s)	172, 292, 382, 1352, 1376
ScaI	AGTACT	1 cut(s)	1399
SchI	GAGTC	2 cut(s)	1274, 1685
ScrFI	CCNGG	6 cut(s)	290, 380, 975, 1623, 1668, 1696
SduI	GDGCHC	6 cut(s)	630, 697, 727, 1145, 1228, 1681
SfaNI	GCATC	3 cut(s)	754, 1177, 1220
Sfr274I	CTCGAG	1 cut(s)	1524
SfuI	TTCGAA	1 cut(s)	1015
SinI	GGWCC	5 cut(s)	172, 292, 382, 1352, 1376
SlaI	CTCGAG	1 cut(s)	1524
SmiMI	CAYNNNNRTG	2 cut(s)	732, 1686
SmlI	CTYRAG	1 cut(s)	1524
SmoI	CTYRAG	1 cut(s)	1524
Sse9I	AATT	8 cut(s)	33, 441, 525, 617, 712, 1011, 1643, 1756
SsiI	CCGC	6 cut(s)	295, 634, 873, 1118, 1239, 1307
SspI	AATATT	1 cut(s)	1322
SspMI	CTAG	5 cut(s)	272, 485, 1167, 1304, 1482
SstI	GAGCTC	1 cut(s)	1681
StyD4I	CCNGG	6 cut(s)	288, 378, 973, 1621, 1666, 1694
StyI	CCWWGG	3 cut(s)	385, 899, 1702
TaaI	ACNGT	4 cut(s)	355, 956, 1223, 1315
TaiI	ACGT	2 cut(s)	452, 1198
TaqI	TCGA	4 cut(s)	189, 1015, 1263, 1525
TasI	AATT	8 cut(s)	33, 441, 525, 617, 712, 1011, 1643, 1756
TatI	WGTACW	4 cut(s)	846, 1218, 1386, 1397
TauI	GCSGC	2 cut(s)	636, 875
TfiI	GAWTC	4 cut(s)	647, 788, 923, 1583
Tru1I	TTAA	5 cut(s)	159, 197, 732, 951, 1133
Tru9I	TTAA	5 cut(s)	159, 197, 732, 951, 1133
TscAI	CASTG	4 cut(s)	684, 1096, 1228, 1558
TseFI	GTSAC	3 cut(s)	97, 284, 1196
TseI	GCWGC	4 cut(s)	118, 869, 929, 1120
Tsp45I	GTSAC	3 cut(s)	97, 284, 1196
TspDTI	ATGAA	4 cut(s)	579, 585, 849, 1806
TspRI	CASTG	4 cut(s)	684, 1096, 1228, 1558
Tth111I	GACNNNGTC	2 cut(s)	95, 131
VneI	GTGCAC	1 cut(s)	1224
VpaK11BI	GGWCC	5 cut(s)	172, 292, 382, 1352, 1376
VspI	ATTAAT	1 cut(s)	732
XapI	RAATTY	5 cut(s)	441, 525, 617, 1643, 1756
XhoI	CTCGAG	1 cut(s)	1524
XspI	CTAG	5 cut(s)	272, 485, 1167, 1304, 1482
ZrmI	AGTACT	1 cut(s)	1399
Zsp2I	ATGCAT	1 cut(s)	157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.