Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008462

Physical Location & Seq

Genomic Coordinates Forward (+)

19079903 .. 19091030

Gene Structure 11128 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1206300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGAAAATCTGCACTTTCTTTATTTTTACCATTGAATTAAAAAAAAGAAGATATCAAATTAAATTAGACAAGTACTTGCTTTGCCGACCCGAAACCGGCGCTCCGAGAGCACCAAAAAACCCGATTGTAACAGAGTTAACTACTGCAACTGTACAACTCTTCCTTCCTTCCTTCCCTACCCGACAGCTAACACCAATTCCGAAACGCATACTGGCTGCGGCAATGGAGGCTCTGAAATCTTCACTTCCAATCTTATCATTCTTCTTGTTGCTCTCACTCCATTCCGATTCTTTCATCTGCACAGCTCTCGCCGACGGTGTCACTCCCGCCGAAGCCAAGCAACTCCGGGACGAGGTCAGGGAAATGTTTTATCATGCATTCAATGGATATATGGAGCATGCTTTTCCTTTGGATGAGTTAAGGCCTCATTCTTGTGCAGGTGAAGATTCACTTGGTGGTTATGCCCTAACTTTGATTGATTCCTTAGACACACTAGCTTTACTTGGTGATCGAGAACACTTTGCCGCCTCTGTTGAATGGATTGGTAAAACACTACGGTTTGATATAAATAAGACAGTATCTGTTTTTGAGACAACCATCCGCGTCCTTGGGGGTTTACTCTCTGCACATCTTATAGCAAGTGATTATTCCACGGGCATGAAAATTCCAACCTATGACAATCAATTACTTACCTTAGCTGAGGATCTTGCCCGGAGATTGTTACCTGCATTTGATACTCCTTCAGGAATTCCTTTTGGATCTGTCAATCTAATGCATGGAGTTGATGAACATGAAAGCAAGATAACATCGACAGCTGGTGGTGGGACCTTGACTTTGGAATTTGGAGTGCTCAGCCGTTTGACAAAAGATCCCATTTTTGAACAAGTTACCAAGAATGCAGTGCGTGGACTTTGGGCTCGCCGTTCAAGGCTCAATTTAGTTGGTGCTCACGTTAATGTTTTTACAGGTGAATGGACACAGAAGGATGCTGGAATAGGAACTAGTATTGACTCTTTCTATGAGTATCTTCTAAAGGCTTATTTATTGTTTGGAGATGAAGAGTATCTTTTCATATTTCAAGAAGCTTATGCCGCTGCTATGCACCATCTTTACAATGATCCTTGGTATGTAGAGGTAAATATGGATTCTGCTGCTGTTGTCTGGCCATTATTTAACAGTTTGCAAGCATTCTGGCCCGGACTTCAGGTTTTAGCTGGAGATATTGATCCTGCCATTCGAACACATGCTGCCTTCTTTAGTGTGTGGAAAAGATATGGTTTTACTCCAGAGGGCTTTAATCTTGCTACGCTCAGTGTGCAGCATGGACAAAGAAGTTATCCACTGCGTCCAGAATTAATGGAGAGCACATATTGGCTCTATAAGGCAACTAGAGATCCAAAGTATCTTGATGCTGGACGAGACATGATTGCCAGCTTGCAGCACAGTGCACGATGCCCCTGTGGATTTTGTCATATATCAGATGTTGAGTTCCACAAGCAGGAAGATCACATGGAGAGCTTTTTCCTGGCAGAAACGGTTAAATACCTGTGGCTTCTTTTTGATTTAGCTGTGGGACCAGATAACCTTGTTGAAAATGGGCCATACAAGTACATATTTAGTACCGAAGGCCACTTATTGCCTGCAACCCCTGAAATAGCTCTTGTGCAGGAACATTGTTCATATTTTGGGGCTTATTGTAACACTGGCAACATTAGAAATGAATCTCAAGCTTCCGATGTTCCAAGTAATTCTCAAGAAACTTATGATGGTAGCTTATATGGAAGGGTCCGCACTGGAATTCCTTCAGACTCGTCTTTGTCGGATTCTACCTCTATATCAGGATTGATAAAAGGGGTTTGCCAAGGACTAACTCATGGGCAGAAATTTGGTATATCATATGTGTCCTCAAGCCACAAAGCTCCTGAGAAGTCCAGAAACCAAAGACAATCGAACGTTGCTGAGAGCCAGTCTGTGATGATTGTCGGAAGCCAAACTTCTGATTATTCATCATCAGAGAACAAGAATTACCATGAGAAATCTCCGGAATCAAGTGACGGAAGACCAGGGAGGAACACACCTCAAAAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004571 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006491 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015923 GO:0015924 GO:0016787 GO:0016798 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0033554 GO:0034976 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0071704 GO:1901135 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Glyco_hydro_47

Protein Analysis

697

Amino Acids

77.63

Weight (kDa)

5.83

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_47	PF01532	122 - 547	4.2e-119	Glycosyl hydrolase family 47

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43710 AT5G43710 AT5G43710
fragaria_vesca	FvH4_4g02250 FvH4_4g02250 FvH4_4g02250 FvH4_4g02250 FvH4_4g02250
malus_domestica	MD16G1206300.v1.1
prunus_persica	Prupe.1G027300_v2.0.a1 Prupe.1G027300_v2.0.a1 Prupe.1G027300_v2.0.a1
pyrus_communis	pycom16g17350
rosa_chinensis	RchiOBHm_Chr4g0389601
rosa_laevigata	RLG00000009969
rosa_multiflora	Rmu_ssc0000478.1_g000010
rosa_roxburghii	Rroxscaffold_5G00335560
rosa_rugosa	Rorug03G0324100
rosa_samantha	Rh4AG028600 Rh4BG021900 Rh4CG030800 Rh4DG022400
rosa_wichuraiana	Rw4G002140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	428
Acc36I	ACCTGC	2 cut(s)	428, 733
AccII	CGCG	1 cut(s)	603
AccIII	TCCGGA	1 cut(s)	2041
AciI	CCGC	6 cut(s)	218, 327, 525, 601, 1092, 1789
AclI	AACGTT	1 cut(s)	1953
AclWI	GGATC	6 cut(s)	711, 766, 863, 1112, 1220, 1388
AcoI	YGGCCR	1 cut(s)	1163
AcsI	RAATTY	5 cut(s)	663, 747, 839, 1797, 1883
AcuI	CTGAAG	3 cut(s)	726, 1187, 1788
AdeI	CACNNNGTG	1 cut(s)	455
AfaI	GTAC	4 cut(s)	74, 153, 1610, 1621
AfiI	CCNNNNNNNGG	3 cut(s)	95, 352, 1498
AgsI	TTSAA	7 cut(s)	35, 382, 536, 881, 927, 1079, 1592
AhlI	ACTAGT	1 cut(s)	1001
AjnI	CCWGG	2 cut(s)	1524, 2062
AjuI	GAANNNNNNNTTGG	2 cut(s)	435, 467
Alw21I	GWGCWC	5 cut(s)	112, 852, 949, 1367, 1450
Alw26I	GTCTC	2 cut(s)	584, 1413
Alw44I	GTGCAC	1 cut(s)	1446
AlwI	GGATC	6 cut(s)	711, 766, 863, 1112, 1220, 1388
AlwNI	CAGNNNCTG	1 cut(s)	581
Aor13HI	TCCGGA	1 cut(s)	2041
AoxI	GGCC	5 cut(s)	422, 1163, 1193, 1598, 1627
ApaLI	GTGCAC	1 cut(s)	1446
ApeKI	GCWGC	6 cut(s)	215, 1094, 1151, 1247, 1318, 1438
ApoI	RAATTY	5 cut(s)	663, 747, 839, 1797, 1883
ArsI	GACNNNNNNTTYG	2 cut(s)	817, 849
AseI	ATTAAT	1 cut(s)	1355
Asp700I	GAANNNNTTC	1 cut(s)	1801
AspLEI	GCGC	1 cut(s)	101
AspS9I	GGNCC	5 cut(s)	825, 1194, 1574, 1598, 1786
AsuC2I	CCSGG	3 cut(s)	347, 712, 1197
AsuHPI	GGTGA	3 cut(s)	452, 518, 980
AsuII	TTCGAA	1 cut(s)	1237
AvaII	GGWCC	3 cut(s)	825, 1574, 1786
BaeGI	GKGCMC	1 cut(s)	1450
BalI	TGGCCA	1 cut(s)	1165
BanII	GRGCYC	1 cut(s)	919
BbsI	GAAGAC	1 cut(s)	2065
Bbv12I	GWGCWC	5 cut(s)	112, 852, 949, 1367, 1450
BbvCI	CCTCAGC	1 cut(s)	699
BbvI	GCAGC	6 cut(s)	202, 1081, 1138, 1234, 1330, 1450
BccI	CCATC	3 cut(s)	605, 1113, 1760
BceAI	ACGGC	2 cut(s)	840, 906
BcgI	CGANNNNNNTGC	2 cut(s)	289, 323
BciT130I	CCWGG	2 cut(s)	1526, 2064
BcnI	CCSGG	3 cut(s)	347, 712, 1197
BcoDI	GTCTC	2 cut(s)	584, 1413
BcuI	ACTAGT	1 cut(s)	1001
BfaI	CTAG	3 cut(s)	494, 1002, 1389
BfoI	RGCGCY	1 cut(s)	102
BfuAI	ACCTGC	2 cut(s)	428, 733
BisI	GCNGC	9 cut(s)	216, 219, 525, 1092, 1095, 1152, 1248, 1319, 1439
BlpI	GCTNAGC	1 cut(s)	851
BlsI	GCNGC	9 cut(s)	217, 220, 526, 1093, 1096, 1153, 1249, 1320, 1440
BmcAI	AGTACT	1 cut(s)	74
Bme1390I	CCNGG	5 cut(s)	347, 712, 1197, 1526, 2064
Bme18I	GGWCC	3 cut(s)	825, 1574, 1786
BmgT120I	GGNCC	5 cut(s)	825, 1194, 1574, 1598, 1786
BmiI	GGNNCC	3 cut(s)	826, 1575, 1787
BmrFI	CCNGG	5 cut(s)	347, 712, 1197, 1526, 2064
BmsI	GCATC	3 cut(s)	976, 1399, 1442
BpiI	GAAGAC	1 cut(s)	2065
BpmI	CTGGAG	2 cut(s)	1236, 1269
Bpu10I	CCTNAGC	2 cut(s)	694, 699
Bpu1102I	GCTNAGC	1 cut(s)	851
Bpu14I	TTCGAA	1 cut(s)	1237
BpuEI	CTTGAG	3 cut(s)	1710, 1737, 1891
BpuMI	CCSGG	3 cut(s)	347, 712, 1197
BsaBI	GATNNNNATC	1 cut(s)	1224
BsaJI	CCNNGG	5 cut(s)	607, 651, 1121, 1861, 2063
BsaWI	WCCGGW	1 cut(s)	2041
BsaXI	ACNNNNNCTCC	2 cut(s)	85, 115
Bsc4I	CCNNNNNNNGG	3 cut(s)	95, 352, 1498
Bse118I	RCCGGY	1 cut(s)	95
Bse1I	ACTGG	4 cut(s)	216, 1708, 1798, 1966
Bse3DI	GCAATG	1 cut(s)	228
Bse8I	GATNNNNATC	1 cut(s)	1224
BseAI	TCCGGA	1 cut(s)	2041
BseBI	CCWGG	2 cut(s)	1526, 2064
BseDI	CCNNGG	5 cut(s)	607, 651, 1121, 1861, 2063
BseGI	GGATG	3 cut(s)	418, 597, 991
BseJI	GATNNNNATC	1 cut(s)	1224
BseLI	CCNNNNNNNGG	3 cut(s)	95, 352, 1498
BseMI	GCAATG	1 cut(s)	228
BseMII	CTCAG	5 cut(s)	690, 865, 1324, 1914, 1950
BseNI	ACTGG	4 cut(s)	216, 1708, 1798, 1966
BseSI	GKGCMC	1 cut(s)	1450
BseXI	GCAGC	6 cut(s)	202, 1081, 1138, 1234, 1330, 1450
BsgI	GTGCAG	5 cut(s)	283, 456, 609, 1337, 1685
Bsh1236I	CGCG	1 cut(s)	603
BshFI	GGCC	5 cut(s)	424, 1165, 1195, 1600, 1629
BsiHKAI	GWGCWC	5 cut(s)	112, 852, 949, 1367, 1450
BsiSI	CCGG	5 cut(s)	96, 346, 712, 1197, 2042
BslFI	GGGAC	3 cut(s)	362, 838, 1587
BslI	CCNNNNNNNGG	3 cut(s)	95, 352, 1498
BsmAI	GTCTC	2 cut(s)	584, 1413
BsmFI	GGGAC	3 cut(s)	362, 838, 1587
BsmI	GAATGC	3 cut(s)	377, 901, 1187
BsnI	GGCC	5 cut(s)	424, 1165, 1195, 1600, 1629
Bsp119I	TTCGAA	1 cut(s)	1237
Bsp1286I	GDGCHC	6 cut(s)	112, 852, 919, 949, 1367, 1450
Bsp13I	TCCGGA	1 cut(s)	2041
Bsp1407I	TGTACA	1 cut(s)	151
Bsp143I	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
Bsp1720I	GCTNAGC	1 cut(s)	851
BspACI	CCGC	6 cut(s)	218, 327, 525, 601, 1092, 1789
BspANI	GGCC	5 cut(s)	424, 1165, 1195, 1600, 1629
BspCNI	CTCAG	5 cut(s)	691, 864, 1323, 1915, 1951
BspEI	TCCGGA	1 cut(s)	2041
BspFNI	CGCG	1 cut(s)	603
BspHI	TCATGA	1 cut(s)	2087
BspLI	GGNNCC	3 cut(s)	826, 1575, 1787
BspMI	ACCTGC	2 cut(s)	428, 733
BspPI	GGATC	6 cut(s)	711, 766, 863, 1112, 1220, 1388
BspT104I	TTCGAA	1 cut(s)	1237
BsrDI	GCAATG	1 cut(s)	228
BsrFI	RCCGGY	1 cut(s)	95
BsrGI	TGTACA	1 cut(s)	151
BsrI	ACTGG	4 cut(s)	216, 1708, 1798, 1966
BssAI	RCCGGY	1 cut(s)	95
BssECI	CCNNGG	5 cut(s)	607, 651, 1121, 1861, 2063
BssMI	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
BssT1I	CCWWGG	3 cut(s)	607, 1121, 1861
Bst2UI	CCWGG	2 cut(s)	1526, 2064
Bst4CI	ACNGT	7 cut(s)	151, 317, 558, 577, 1178, 1445, 1537
Bst6I	CTCTTC	2 cut(s)	164, 1053
BstAUI	TGTACA	1 cut(s)	151
BstBI	TTCGAA	1 cut(s)	1237
BstC8I	GCNNGC	6 cut(s)	399, 919, 1185, 1432, 1436, 1641
BstDEI	CTNAG	7 cut(s)	484, 694, 699, 851, 1310, 1923, 1959
BstDSI	CCRYGG	1 cut(s)	651
BstF5I	GGATG	3 cut(s)	418, 597, 991
BstFNI	CGCG	1 cut(s)	603
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	1 cut(s)	101
BstKTI	GATC	8 cut(s)	511, 706, 761, 871, 1120, 1228, 1396, 1507
BstMAI	GTCTC	2 cut(s)	584, 1413
BstMBI	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
BstMWI	GCNNNNNNNGC	4 cut(s)	107, 227, 1091, 1315
BstNI	CCWGG	2 cut(s)	1526, 2064
BstNSI	RCATGY	2 cut(s)	401, 1247
BstSCI	CCNGG	5 cut(s)	345, 710, 1195, 1524, 2062
BstSLI	GKGCMC	1 cut(s)	1450
BstUI	CGCG	1 cut(s)	603
BstV1I	GCAGC	6 cut(s)	202, 1081, 1138, 1234, 1330, 1450
BstV2I	GAAGAC	1 cut(s)	2065
BstX2I	RGATCY	4 cut(s)	703, 758, 868, 1393
BstYI	RGATCY	4 cut(s)	703, 758, 868, 1393
BsuRI	GGCC	5 cut(s)	424, 1165, 1195, 1600, 1629
BtgI	CCRYGG	1 cut(s)	651
BtsCI	GGATG	3 cut(s)	418, 597, 991
BtsI	GCAGTG	2 cut(s)	906, 1340
BtsIMutI	CAGTG	6 cut(s)	906, 1318, 1340, 1450, 1701, 1791
BveI	ACCTGC	2 cut(s)	428, 733
Cac8I	GCNNGC	6 cut(s)	399, 919, 1185, 1432, 1436, 1641
CaiI	CAGNNNCTG	1 cut(s)	581
CciI	TCATGA	1 cut(s)	2087
CfoI	GCGC	1 cut(s)	101
Cfr10I	RCCGGY	1 cut(s)	95
Cfr13I	GGNCC	5 cut(s)	825, 1194, 1574, 1598, 1786
CseI	GACGC	2 cut(s)	592, 1334
Csp6I	GTAC	4 cut(s)	73, 152, 1609, 1620
CviQI	GTAC	4 cut(s)	73, 152, 1609, 1620
DdeI	CTNAG	7 cut(s)	484, 694, 699, 851, 1310, 1923, 1959
DpnI	GATC	8 cut(s)	510, 705, 760, 870, 1119, 1227, 1395, 1506
DpnII	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
DraIII	CACNNNGTG	1 cut(s)	455
EaeI	YGGCCR	1 cut(s)	1163
Eam1104I	CTCTTC	2 cut(s)	164, 1053
EarI	CTCTTC	2 cut(s)	164, 1053
Eco130I	CCWWGG	3 cut(s)	607, 1121, 1861
Eco147I	AGGCCT	1 cut(s)	424
Eco24I	GRGCYC	1 cut(s)	919
Eco32I	GATATC	1 cut(s)	53
Eco47I	GGWCC	3 cut(s)	825, 1574, 1786
Eco57I	CTGAAG	3 cut(s)	726, 1187, 1788
EcoO109I	RGGNCCY	1 cut(s)	825
EcoRI	GAATTC	2 cut(s)	747, 1797
EcoRII	CCWGG	2 cut(s)	1524, 2062
EcoRV	GATATC	1 cut(s)	53
EcoT14I	CCWWGG	3 cut(s)	607, 1121, 1861
EcoT22I	ATGCAT	2 cut(s)	379, 777
EcoT38I	GRGCYC	1 cut(s)	919
ErhI	CCWWGG	3 cut(s)	607, 1121, 1861
FalI	AAGNNNNNCTT	6 cut(s)	435, 467, 1050, 1082, 1978, 2010
FaqI	GGGAC	3 cut(s)	362, 838, 1587
FauI	CCCGC	1 cut(s)	334
FauNDI	CATATG	1 cut(s)	1897
Fnu4HI	GCNGC	9 cut(s)	216, 219, 525, 1092, 1095, 1152, 1248, 1319, 1439
FokI	GGATG	3 cut(s)	425, 584, 998
FriOI	GRGCYC	1 cut(s)	919
Fsp4HI	GCNGC	9 cut(s)	216, 219, 525, 1092, 1095, 1152, 1248, 1319, 1439
FspBI	CTAG	3 cut(s)	494, 1002, 1389
GlaI	GCGC	1 cut(s)	100
GluI	GCNGC	9 cut(s)	216, 219, 525, 1092, 1095, 1152, 1248, 1319, 1439
GsuI	CTGGAG	2 cut(s)	1236, 1269
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	5 cut(s)	424, 1165, 1195, 1600, 1629
HapII	CCGG	5 cut(s)	96, 346, 712, 1197, 2042
HgaI	GACGC	2 cut(s)	592, 1334
HhaI	GCGC	1 cut(s)	101
Hin6I	GCGC	1 cut(s)	99
HinP1I	GCGC	1 cut(s)	99
HincII	GTYRAC	1 cut(s)	138
HindII	GTYRAC	1 cut(s)	138
HindIII	AAGCTT	2 cut(s)	1083, 1728
HinfI	GANTC	9 cut(s)	287, 446, 479, 1010, 1145, 1721, 1808, 1823, 2045
HpaI	GTTAAC	1 cut(s)	138
HpaII	CCGG	5 cut(s)	96, 346, 712, 1197, 2042
HphI	GGTGA	3 cut(s)	452, 518, 980
Hpy166II	GTNNAC	4 cut(s)	138, 617, 908, 1448
Hpy8I	GTNNAC	4 cut(s)	138, 617, 908, 1448
Hpy99I	CGWCG	1 cut(s)	317
HpyAV	CCTTC	9 cut(s)	173, 177, 181, 750, 976, 1261, 1619, 1776, 1812
HpyCH4III	ACNGT	7 cut(s)	151, 317, 558, 577, 1178, 1445, 1537
HpyCH4IV	ACGT	2 cut(s)	951, 1953
HpyF10VI	GCNNNNNNNGC	4 cut(s)	107, 227, 1091, 1315
HpyF3I	CTNAG	7 cut(s)	484, 694, 699, 851, 1310, 1923, 1959
HpySE526I	ACGT	2 cut(s)	951, 1953
HspAI	GCGC	1 cut(s)	99
Kpn2I	TCCGGA	1 cut(s)	2041
KspAI	GTTAAC	1 cut(s)	138
Kzo9I	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
LmnI	GCTCC	3 cut(s)	106, 394, 1924
Lsp1109I	GCAGC	6 cut(s)	202, 1081, 1138, 1234, 1330, 1450
LweI	GCATC	3 cut(s)	976, 1399, 1442
MaeI	CTAG	3 cut(s)	494, 1002, 1389
MaeII	ACGT	2 cut(s)	951, 1953
MaeIII	GTNAC	6 cut(s)	127, 319, 720, 886, 1697, 2051
MalI	GATC	8 cut(s)	510, 705, 760, 870, 1119, 1227, 1395, 1506
MboI	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
MboII	GAAGA	9 cut(s)	60, 151, 231, 253, 455, 1019, 1070, 1514, 2070
MflI	RGATCY	4 cut(s)	703, 758, 868, 1393
MhlI	GDGCHC	6 cut(s)	112, 852, 919, 949, 1367, 1450
MlsI	TGGCCA	1 cut(s)	1165
MluNI	TGGCCA	1 cut(s)	1165
MlyI	GAGTC	2 cut(s)	1004, 1802
MmeI	TCCRAC	3 cut(s)	692, 1800, 1963
Mox20I	TGGCCA	1 cut(s)	1165
Mph1103I	ATGCAT	2 cut(s)	379, 777
MroI	TCCGGA	1 cut(s)	2041
MroXI	GAANNNNTTC	1 cut(s)	1801
MscI	TGGCCA	1 cut(s)	1165
MseI	TTAA	9 cut(s)	38, 60, 137, 419, 954, 1173, 1296, 1355, 1539
MslI	CAYNNNNRTG	3 cut(s)	432, 656, 954
Msp20I	TGGCCA	1 cut(s)	1165
MspA1I	CMGCKG	2 cut(s)	815, 1094
MspI	CCGG	5 cut(s)	96, 346, 712, 1197, 2042
MspR9I	CCNGG	5 cut(s)	347, 712, 1197, 1526, 2064
Mva1269I	GAATGC	3 cut(s)	377, 901, 1187
MvaI	CCWGG	2 cut(s)	1526, 2064
MvnI	CGCG	1 cut(s)	603
MwoI	GCNNNNNNNGC	4 cut(s)	107, 227, 1091, 1315
NciI	CCSGG	3 cut(s)	347, 712, 1197
NdeI	CATATG	1 cut(s)	1897
NdeII	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
NlaIV	GGNNCC	3 cut(s)	826, 1575, 1787
NmuCI	GTSAC	2 cut(s)	319, 2051
NsiI	ATGCAT	2 cut(s)	379, 777
NspI	RCATGY	2 cut(s)	401, 1247
NspV	TTCGAA	1 cut(s)	1237
PaeI	GCATGC	1 cut(s)	401
PagI	TCATGA	1 cut(s)	2087
PaqCI	CACCTGC	1 cut(s)	428
PceI	AGGCCT	1 cut(s)	424
PctI	GAATGC	3 cut(s)	377, 901, 1187
PdmI	GAANNNNTTC	1 cut(s)	1801
PfeI	GAWTC	7 cut(s)	287, 446, 479, 1145, 1721, 1823, 2045
PflFI	GACNNNGTC	2 cut(s)	317, 353
PfoI	TCCNGGA	1 cut(s)	345
PkrI	GCNGC	9 cut(s)	217, 220, 526, 1093, 1096, 1153, 1249, 1320, 1440
PleI	GAGTC	2 cut(s)	1004, 1802
PpsI	GAGTC	2 cut(s)	1004, 1802
PpuMI	RGGWCCY	1 cut(s)	825
PshBI	ATTAAT	1 cut(s)	1355
Psp1406I	AACGTT	1 cut(s)	1953
Psp5II	RGGWCCY	1 cut(s)	825
Psp6I	CCWGG	2 cut(s)	1524, 2062
PspGI	CCWGG	2 cut(s)	1524, 2062
PspN4I	GGNNCC	3 cut(s)	826, 1575, 1787
PspPI	GGNCC	5 cut(s)	825, 1194, 1574, 1598, 1786
PspPPI	RGGWCCY	1 cut(s)	825
PsrI	GAACNNNNNNTAC	2 cut(s)	2009, 2041
PstNI	CAGNNNCTG	1 cut(s)	581
PsuI	RGATCY	4 cut(s)	703, 758, 868, 1393
PsyI	GACNNNGTC	2 cut(s)	317, 353
PvuII	CAGCTG	1 cut(s)	815
RsaI	GTAC	4 cut(s)	74, 153, 1610, 1621
RsaNI	GTAC	4 cut(s)	73, 152, 1609, 1620
RseI	CAYNNNNRTG	3 cut(s)	432, 656, 954
SaqAI	TTAA	9 cut(s)	38, 60, 137, 419, 954, 1173, 1296, 1355, 1539
SatI	GCNGC	9 cut(s)	216, 219, 525, 1092, 1095, 1152, 1248, 1319, 1439
Sau3AI	GATC	8 cut(s)	508, 703, 758, 868, 1117, 1225, 1393, 1504
Sau96I	GGNCC	5 cut(s)	825, 1194, 1574, 1598, 1786
ScaI	AGTACT	1 cut(s)	74
SchI	GAGTC	2 cut(s)	1004, 1802
ScrFI	CCNGG	5 cut(s)	347, 712, 1197, 1526, 2064
SduI	GDGCHC	6 cut(s)	112, 852, 919, 949, 1367, 1450
SfaNI	GCATC	3 cut(s)	976, 1399, 1442
SfuI	TTCGAA	1 cut(s)	1237
SinI	GGWCC	3 cut(s)	825, 1574, 1786
SmiMI	CAYNNNNRTG	3 cut(s)	432, 656, 954
SmlI	CTYRAG	3 cut(s)	1725, 1752, 1906
SmoI	CTYRAG	3 cut(s)	1725, 1752, 1906
SpeI	ACTAGT	1 cut(s)	1001
SphI	GCATGC	1 cut(s)	401
SseBI	AGGCCT	1 cut(s)	424
SsiI	CCGC	6 cut(s)	218, 327, 525, 601, 1092, 1789
SspMI	CTAG	3 cut(s)	494, 1002, 1389
StuI	AGGCCT	1 cut(s)	424
StyD4I	CCNGG	5 cut(s)	345, 710, 1195, 1524, 2062
StyI	CCWWGG	3 cut(s)	607, 1121, 1861
TaaI	ACNGT	7 cut(s)	151, 317, 558, 577, 1178, 1445, 1537
TaiI	ACGT	2 cut(s)	954, 1956
TaqI	TCGA	4 cut(s)	511, 809, 1237, 1949
TatI	WGTACW	3 cut(s)	72, 151, 1608
TauI	GCSGC	3 cut(s)	221, 527, 1094
TfiI	GAWTC	7 cut(s)	287, 446, 479, 1145, 1721, 1823, 2045
Tru1I	TTAA	9 cut(s)	38, 60, 137, 419, 954, 1173, 1296, 1355, 1539
Tru9I	TTAA	9 cut(s)	38, 60, 137, 419, 954, 1173, 1296, 1355, 1539
TscAI	CASTG	6 cut(s)	906, 1318, 1347, 1450, 1708, 1798
TseFI	GTSAC	2 cut(s)	319, 2051
TseI	GCWGC	6 cut(s)	215, 1094, 1151, 1247, 1318, 1438
Tsp45I	GTSAC	2 cut(s)	319, 2051
TspGWI	ACGGA	1 cut(s)	2070
TspRI	CASTG	6 cut(s)	906, 1318, 1347, 1450, 1708, 1798
Tth111I	GACNNNGTC	2 cut(s)	317, 353
VneI	GTGCAC	1 cut(s)	1446
VpaK11BI	GGWCC	3 cut(s)	825, 1574, 1786
VspI	ATTAAT	1 cut(s)	1355
XapI	RAATTY	5 cut(s)	663, 747, 839, 1797, 1883
XceI	RCATGY	2 cut(s)	401, 1247
XmnI	GAANNNNTTC	1 cut(s)	1801
XspI	CTAG	3 cut(s)	494, 1002, 1389
ZrmI	AGTACT	1 cut(s)	74
Zsp2I	ATGCAT	2 cut(s)	379, 777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.