FvH4_4g08450

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018298
Physical Location & Seq
Forward (+)
8458698 .. 8459692
995 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08450.t1

Sequence Viewer

Length: 549 bp
ATGGCAGCAAGAGAGCCTTTTAACGTGACAGTCAGGTCACCGGCCGTGACCACTCAGCGGTCACCGTGGCATTCGCCGGTCCCTTACCTTTTCGGCGGTTTGGCGGCCATGTTGGGCCTCATCGCCTTTGCCCTTCTCATCTTAGCCTGTTCTTACTGGAAGCTTTCCGGCTACCTCGAGAACAGCGAAAACGACGGCGAGCGAGACCTGGAGGCCGGCGAAGGAGGCAAAGGCGACGAGACCCAGAAGGCCCCGCCGGTTTTCGAAGAGAAAATATTGGTGATCATGGCCGGTGACGCCAAGCCAACTTTCTTGGCCACCCCCATTTCGAGTAGGTCATCGTCTTTCGGGGACAGTTCGAGTACTAATAGCACAAGTACTTGTAGCTGCGGGAAGAGTGACAAGTCGGTGGAAATGGCCGAGCAGACTGAGACGACGACTGTGAAATTGGAAACAAGTGGCGGCGCTGAAAATGTTCATCATCATCATGAGAACAGCACCGAGGCGACGCATGAGGCCTCAACAGATCAGACTCATCAGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

19.45

Weight (kDa)

4.98

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08450
rosa_chinensis RchiOBHm_Chr4g0403911
rosa_laevigata RLG00000008942
rosa_multiflora Rmu_sc0005134.1_g000010
rosa_roxburghii Rroxscaffold_5G00348530
rosa_rugosa Rorug04G0046300
rosa_samantha Rh4BG114900 Rh4CG130200 Rh4DG114900
rosa_wichuraiana Rw4G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
AciI CCGC 6 cut(s) 58, 96, 104, 254, 390, 462
AcoI YGGCCR 5 cut(s) 42, 105, 288, 315, 417
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 364, 379
AfiI CCNNNNNNNGG 1 cut(s) 57
AjnI CCWGG 1 cut(s) 207
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 2 cut(s) 163, 387
AluI AGCT 2 cut(s) 163, 387
Alw26I GTCTC 3 cut(s) 198, 233, 425
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 9 cut(s) 42, 105, 115, 213, 249, 288, 315, 417, 516
ApeKI GCWGC 2 cut(s) 5, 387
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 79, 115, 250
AsuHPI GGTGA 4 cut(s) 30, 54, 292, 305
AsuII TTCGAA 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 79
BalI TGGCCA 1 cut(s) 317
BbvI GCAGC 2 cut(s) 17, 374
BceAI ACGGC 2 cut(s) 29, 211
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 3 cut(s) 198, 233, 425
BfoI RGCGCY 1 cut(s) 468
BisI GCNGC 4 cut(s) 6, 105, 388, 463
BlsI GCNGC 4 cut(s) 7, 106, 389, 464
BmcAI AGTACT 2 cut(s) 364, 379
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 79, 115, 250
BmiI GGNNCC 2 cut(s) 81, 252
BmrFI CCNGG 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 2 cut(s) 198, 233
BsaJI CCNNGG 2 cut(s) 65, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 5 cut(s) 40, 76, 215, 256, 290
Bse1I ACTGG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 65, 501
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 68, 420
BseNI ACTGG 1 cut(s) 161
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 17, 374
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 9 cut(s) 44, 107, 117, 215, 251, 290, 317, 419, 518
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 6 cut(s) 41, 77, 168, 216, 257, 291
BslFI GGGAC 2 cut(s) 65, 365
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 3 cut(s) 198, 233, 425
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 2 cut(s) 65, 365
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 9 cut(s) 44, 107, 117, 215, 251, 290, 317, 419, 518
Bso31I GGTCTC 2 cut(s) 198, 233
BsoBI CYCGRG 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 264
Bsp143I GATC 2 cut(s) 282, 526
BspACI CCGC 6 cut(s) 58, 96, 104, 254, 390, 462
BspANI GGCC 9 cut(s) 44, 107, 117, 215, 251, 290, 317, 419, 518
BspCNI CTCAG 2 cut(s) 67, 421
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 81, 252
BspT104I TTCGAA 1 cut(s) 264
BspTNI GGTCTC 2 cut(s) 198, 233
BsrFI RCCGGY 5 cut(s) 40, 76, 215, 256, 290
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 5 cut(s) 40, 76, 215, 256, 290
BssECI CCNNGG 2 cut(s) 65, 501
BssMI GATC 2 cut(s) 282, 526
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 4 cut(s) 31, 66, 356, 442
Bst6I CTCTTC 2 cut(s) 261, 389
BstACI GRCGYC 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 200, 217
BstDEI CTNAG 3 cut(s) 54, 142, 429
BstDSI CCRYGG 1 cut(s) 65
BstEII GGTNACC 2 cut(s) 36, 60
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 2 cut(s) 285, 529
BstMAI GTCTC 3 cut(s) 198, 233, 425
BstMBI GATC 2 cut(s) 282, 526
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 225, 296
BstNI CCWGG 1 cut(s) 209
BstPI GGTNACC 2 cut(s) 36, 60
BstSCI CCNGG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 17, 374
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 9 cut(s) 44, 107, 117, 215, 251, 290, 317, 419, 518
BtgI CCRYGG 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 200, 217
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 5 cut(s) 40, 76, 215, 256, 290
Cfr13I GGNCC 3 cut(s) 79, 115, 250
CseI GACGC 2 cut(s) 305, 517
Csp6I GTAC 2 cut(s) 363, 378
CviAII CATG 4 cut(s) 109, 286, 488, 512
CviQI GTAC 2 cut(s) 363, 378
DdeI CTNAG 3 cut(s) 54, 142, 429
DpnI GATC 2 cut(s) 284, 528
DpnII GATC 2 cut(s) 282, 526
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
EaeI YGGCCR 5 cut(s) 42, 105, 288, 315, 417
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 261, 389
EarI CTCTTC 2 cut(s) 261, 389
EclXI CGGCCG 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 518
Eco31I GGTCTC 2 cut(s) 198, 233
Eco47I GGWCC 1 cut(s) 79
Eco52I CGGCCG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 176
Eco91I GGTNACC 2 cut(s) 36, 60
EcoO109I RGGNCCY 1 cut(s) 250
EcoO65I GGTNACC 2 cut(s) 36, 60
EcoRII CCWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 4 cut(s) 112, 289, 491, 515
FaiI YATR 4 cut(s) 110, 287, 489, 513
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 2 cut(s) 65, 365
FatI CATG 4 cut(s) 108, 285, 487, 511
FauI CCCGC 2 cut(s) 261, 383
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 6, 105, 388, 463
Fsp4HI GCNGC 4 cut(s) 6, 105, 388, 463
GlaI GCGC 1 cut(s) 466
GluI GCNGC 4 cut(s) 6, 105, 388, 463
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 9 cut(s) 44, 107, 117, 215, 251, 290, 317, 419, 518
HapII CCGG 6 cut(s) 41, 77, 168, 216, 257, 291
HgaI GACGC 2 cut(s) 305, 517
HhaI GCGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 4 cut(s) 112, 289, 491, 515
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 1 cut(s) 532
HpaII CCGG 6 cut(s) 41, 77, 168, 216, 257, 291
HphI GGTGA 4 cut(s) 30, 54, 292, 305
Hpy188I TCNGA 2 cut(s) 531, 540
Hpy188III TCNNGA 2 cut(s) 178, 488
Hpy99I CGWCG 4 cut(s) 197, 239, 439, 511
HpyAV CCTTC 3 cut(s) 143, 215, 241
HpyCH4III ACNGT 4 cut(s) 31, 66, 356, 442
HpyCH4IV ACGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 296
HpyF3I CTNAG 3 cut(s) 54, 142, 429
HpySE526I ACGT 1 cut(s) 24
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 4 cut(s) 112, 289, 491, 515
HspAI GCGC 1 cut(s) 465
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 2 cut(s) 282, 526
Lsp1109I GCAGC 2 cut(s) 17, 374
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 6 cut(s) 25, 36, 46, 60, 293, 398
MalI GATC 2 cut(s) 284, 528
MboI GATC 2 cut(s) 282, 526
MboII GAAGA 2 cut(s) 278, 406
MfeI CAATTG 1 cut(s) 544
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 446, 544
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 1 cut(s) 526
MnlI CCTC 7 cut(s) 128, 185, 205, 218, 496, 508, 529
Mox20I TGGCCA 1 cut(s) 317
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 317
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 6 cut(s) 41, 77, 168, 216, 257, 291
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 544
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 225, 296
NaeI GCCGGC 1 cut(s) 217
NdeII GATC 2 cut(s) 282, 526
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 4 cut(s) 112, 289, 491, 515
NlaIV GGNNCC 2 cut(s) 81, 252
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 6 cut(s) 25, 36, 46, 60, 293, 398
NspV TTCGAA 1 cut(s) 264
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 70
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 474
PkrI GCNGC 4 cut(s) 7, 106, 389, 464
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 207
PspEI GGTNACC 2 cut(s) 36, 60
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 81, 252
PspPI GGNCC 3 cut(s) 79, 115, 250
RsaI GTAC 2 cut(s) 364, 379
RsaNI GTAC 2 cut(s) 363, 378
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 4 cut(s) 6, 105, 388, 463
Sau3AI GATC 2 cut(s) 282, 526
Sau96I GGNCC 3 cut(s) 79, 115, 250
ScaI AGTACT 2 cut(s) 364, 379
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 209
SetI ASST 8 cut(s) 27, 38, 90, 165, 177, 210, 338, 389
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 264
SinI GGWCC 1 cut(s) 79
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 2 cut(s) 446, 544
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 6 cut(s) 58, 96, 104, 254, 390, 462
SspI AATATT 1 cut(s) 276
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 4 cut(s) 31, 66, 356, 442
TaiI ACGT 1 cut(s) 27
TaqI TCGA 4 cut(s) 177, 264, 329, 359
TasI AATT 2 cut(s) 446, 544
TatI WGTACW 2 cut(s) 362, 377
TauI GCSGC 2 cut(s) 107, 465
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 6 cut(s) 25, 36, 46, 60, 293, 398
TseI GCWGC 2 cut(s) 5, 387
Tsp45I GTSAC 6 cut(s) 25, 36, 46, 60, 293, 398
TspDTI ATGAA 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 474
ZrmI AGTACT 2 cut(s) 364, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.