Rorug04G0046300

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0018298
Physical Location & Seq
Reverse (-)
7209357 .. 7212237
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0046300.1

Sequence Viewer

Length: 678 bp
ATGGATGGAATGGAAGGCATAGAAGACTTTTTTGGGTACCCCAGCTTGATCGACGAACCCCAATTCCCCAATTTTCTCTGGTCCAATCCCAGCCCTTCATTTCCTGAGATCGAATTCTCTTCTTATAGTGGTGTTGCTGCTCCCTCACAGACCCAGGAAAAACAGTGCCCTCGCAAGAGGGGACGAACCGAGTCTTGGAGTGGCTCAGGAGCCAAAGCTTGCCGTGAGAAATTGAGGAGGGAGAGATTGAATGACAAGTTTGTGGATTTGAGTGCTGTTTTGGAACCTGGGAGGCCAGCCAAGACTGACAAGCCTGCTATACTTGATGATGCCATTAGGGTCTTGACCCAACTGAGAGCTGAAGCTCAGGAGATTACACAGACAAATGAAAAGTTGCTTGAAGAGATAAAGAGTTTAAAGGCAGAAAAGAATGAACTTCGTGAAGAGAAAGTTGTACTGAAAGCAGACAAAGAAAAGCTGGAACAGCAGTTGAAAGCTATGACCATTCCACCTTCTGGGTTTATGCCAGCCCACCCAGCAGTCCCTGCCGCATATCACCCTGGGGCAAGCAAGATGGCAGTCTATCCTAGCTATGGCATGATTCCAATGTGGCACTATTTACCTCCATCAACCCGTGATACATCTCGTGATCACGAGCTTAGGCCTCCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.28

Weight (kDa)

6.15

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 71 - 119 5.3e-08 Helix-loop-helix DNA-binding domain
bHLH_IRO3 PF23177 72 - 136 4.6e-07 Iron-related transcription factor 3 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08450
rosa_chinensis RchiOBHm_Chr4g0403911
rosa_laevigata RLG00000008942
rosa_multiflora Rmu_sc0005134.1_g000010
rosa_roxburghii Rroxscaffold_5G00348530
rosa_rugosa Rorug04G0046300
rosa_samantha Rh4BG114900 Rh4CG130200 Rh4DG114900
rosa_wichuraiana Rw4G009910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 36
AccB7I CCANNNNNTGG 1 cut(s) 515
AciI CCGC 1 cut(s) 549
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 2 cut(s) 38, 456
AfiI CCNNNNNNNGG 3 cut(s) 195, 515, 593
AgsI TTSAA 3 cut(s) 250, 401, 493
AjnI CCWGG 3 cut(s) 153, 286, 559
AjuI GAANNNNNNNTTGG 4 cut(s) 15, 47, 178, 210
AloI GAACNNNNNNTCC 2 cut(s) 48, 80
AluBI AGCT 8 cut(s) 45, 218, 359, 365, 478, 497, 591, 658
AluI AGCT 8 cut(s) 45, 218, 359, 365, 478, 497, 591, 658
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 2 cut(s) 293, 662
ApeKI GCWGC 2 cut(s) 137, 671
ApoI RAATTY 1 cut(s) 113
Asp718I GGTACC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 548
AvaII GGWCC 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 36
BauI CACGAG 2 cut(s) 645, 653
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 124, 658
BccI CCATC 2 cut(s) 568, 634
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 3 cut(s) 155, 288, 561
BclI TGATCA 1 cut(s) 649
BfaI CTAG 1 cut(s) 588
BisI GCNGC 3 cut(s) 138, 549, 672
BlsI GCNGC 3 cut(s) 139, 550, 673
Bme1390I CCNGG 3 cut(s) 155, 288, 561
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 3 cut(s) 38, 211, 285
BmrFI CCNGG 3 cut(s) 155, 288, 561
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 30
Bpu10I CCTNAGC 3 cut(s) 205, 366, 659
BsaJI CCNNGG 4 cut(s) 153, 287, 559, 560
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 515, 593
BseBI CCWGG 3 cut(s) 155, 288, 561
BseDI CCNNGG 4 cut(s) 153, 287, 559, 560
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 195, 515, 593
BseMII CTCAG 4 cut(s) 96, 219, 344, 380
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 2 cut(s) 124, 658
BseYI CCCAGC 3 cut(s) 41, 89, 535
BshFI GGCC 2 cut(s) 295, 664
BshNI GGYRCC 1 cut(s) 36
BslFI GGGAC 2 cut(s) 195, 527
BslI CCNNNNNNNGG 3 cut(s) 195, 515, 593
BsmFI GGGAC 2 cut(s) 195, 527
BsnI GGCC 2 cut(s) 295, 664
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 3 cut(s) 48, 108, 649
BspACI CCGC 1 cut(s) 549
BspANI GGCC 2 cut(s) 295, 664
BspCNI CTCAG 4 cut(s) 97, 218, 345, 379
BspLI GGNNCC 3 cut(s) 38, 211, 285
BspT107I GGYRCC 1 cut(s) 36
BssECI CCNNGG 4 cut(s) 153, 287, 559, 560
BssMI GATC 3 cut(s) 48, 108, 649
BssSI CACGAG 2 cut(s) 645, 653
Bst2BI CACGAG 2 cut(s) 645, 653
Bst2UI CCWGG 3 cut(s) 155, 288, 561
Bst4CI ACNGT 1 cut(s) 165
Bst6I CTCTTC 3 cut(s) 124, 396, 438
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 5 cut(s) 220, 297, 315, 528, 568
BstDEI CTNAG 6 cut(s) 105, 205, 353, 366, 659, 675
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 3 cut(s) 51, 111, 652
BstMBI GATC 3 cut(s) 48, 108, 649
BstMWI GCNNNNNNNGC 3 cut(s) 484, 536, 545
BstNI CCWGG 3 cut(s) 155, 288, 561
BstSCI CCNGG 3 cut(s) 153, 286, 559
BstSLI GKGCMC 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 124, 658
BstV2I GAAGAC 1 cut(s) 30
BsuRI GGCC 2 cut(s) 295, 664
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 5 cut(s) 220, 297, 315, 528, 568
CaiI CAGNNNCTG 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 81
Csp6I GTAC 2 cut(s) 37, 455
CviAII CATG 1 cut(s) 598
CviQI GTAC 2 cut(s) 37, 455
DdeI CTNAG 6 cut(s) 105, 205, 353, 366, 659, 675
DpnI GATC 3 cut(s) 50, 110, 651
DpnII GATC 3 cut(s) 48, 108, 649
DraI TTTAAA 1 cut(s) 417
Eam1104I CTCTTC 3 cut(s) 124, 396, 438
EarI CTCTTC 3 cut(s) 124, 396, 438
Eco147I AGGCCT 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 153, 286, 559
FaeI CATG 1 cut(s) 601
FaiI YATR 8 cut(s) 20, 126, 320, 500, 524, 553, 594, 599
FaqI GGGAC 2 cut(s) 195, 527
FatI CATG 1 cut(s) 597
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 3 cut(s) 138, 549, 672
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 138, 549, 672
FspBI CTAG 1 cut(s) 588
GluI GCNGC 3 cut(s) 138, 549, 672
GsaI CCCAGC 3 cut(s) 45, 93, 539
HaeIII GGCC 2 cut(s) 295, 664
Hin1II CATG 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 2 cut(s) 191, 601
HphI GGTGA 1 cut(s) 548
Hpy188III TCNNGA 7 cut(s) 104, 207, 343, 368, 440, 647, 653
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 3 cut(s) 8, 105, 522
HpyCH4III ACNGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 3 cut(s) 484, 536, 545
HpyF3I CTNAG 6 cut(s) 105, 205, 353, 366, 659, 675
Hsp92II CATG 1 cut(s) 601
KpnI GGTACC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 3 cut(s) 48, 108, 649
LmnI GCTCC 2 cut(s) 145, 209
Lsp1109I GCAGC 2 cut(s) 124, 658
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 588
MalI GATC 3 cut(s) 50, 110, 651
MboI GATC 3 cut(s) 48, 108, 649
MboII GAAGA 4 cut(s) 35, 111, 413, 455
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 4 cut(s) 62, 70, 113, 230
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 8 cut(s) 154, 171, 180, 228, 231, 285, 633, 675
MseI TTAA 1 cut(s) 416
MspR9I CCNGG 3 cut(s) 155, 288, 561
MvaI CCWGG 3 cut(s) 155, 288, 561
MwoI GCNNNNNNNGC 3 cut(s) 484, 536, 545
NdeII GATC 3 cut(s) 48, 108, 649
NlaIII CATG 1 cut(s) 601
NlaIV GGNNCC 3 cut(s) 38, 211, 285
PasI CCCWGGG 1 cut(s) 560
PceI AGGCCT 1 cut(s) 664
PfeI GAWTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 515
PkrI GCNGC 3 cut(s) 139, 550, 673
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Psp6I CCWGG 3 cut(s) 153, 286, 559
PspFI CCCAGC 3 cut(s) 41, 89, 535
PspGI CCWGG 3 cut(s) 153, 286, 559
PspN4I GGNNCC 3 cut(s) 38, 211, 285
PspPI GGNCC 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 38, 456
RsaNI GTAC 2 cut(s) 37, 455
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 3 cut(s) 138, 549, 672
Sau3AI GATC 3 cut(s) 48, 108, 649
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 155, 288, 561
SduI GDGCHC 1 cut(s) 170
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 81
Sse9I AATT 4 cut(s) 62, 70, 113, 230
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 1 cut(s) 549
SspMI CTAG 1 cut(s) 588
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 3 cut(s) 153, 286, 559
TaaI ACNGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 51, 111
TasI AATT 4 cut(s) 62, 70, 113, 230
TatI WGTACW 1 cut(s) 454
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 1 cut(s) 170
TseI GCWGC 2 cut(s) 137, 671
TspDTI ATGAA 3 cut(s) 87, 402, 447
TspRI CASTG 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 1 cut(s) 113
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.