FvH4_4g12530

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016822
Physical Location & Seq
Reverse (-)
16206291 .. 16214549
8259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12530.t1

Sequence Viewer

Length: 1851 bp
ATGAGGATGGAGGAGGGTGCTGAGAAGCCCATAGAGGTGAGGGCTCTGGAGGCTCTTGGGCAGGGGTTTGATCTGAGCAGTGATTTCAGGTTGAAGTTTGCAAAAGGTTTGGATGGGAGTAAAGGGAGGTTGGTGGTTTTGAATGAGGAGAGTAAGAGGGACATTGTCATTCCTAGTGGTAGTGGTGGGGGTGTTGTCATTCATGGTGTTCCTCAGGATATTCATTGTGATAAAGGGGATCGAATCCGCTTCAAATCCGATGTTCTTGAATTCAATCAGATGTCTGAGCTACTTAATCAGAAATCTTCAGTTCAAGGGAAAATTCCTTCAGGATATCTGAATTCCATTTTTGATTTAACTGGAGACTGGCTTCAAGATATCGCAGAAACAAAATATCTTGCTTTTGATGGTTTCTTCATTTCATTGTACAATTTGCACCTAACAGCATCTCCACTAATCTTGCGAAACAAAGTACAGAAATCTGTTCCAGCTCGTTGGGATCCAGCTGCATTGTCGAGGTTCATCCGTTCATATGGGACACACATAATAGTTGGGATGGCAGTTGGAGGTCAAGATTTACTTTGTGTTAGGCAGAAATCTTCCTCACCAGTTCCTCCCGCTGATCTCAGAAGACACCTAGAGGATCTTGGAGATTTTCTATTTTCAGATGGACCTTCTTTACTACGGGGAAAAACAAGAAATGGAAAACAAAATATTCCGGAGGTGTTTGATCGTATTTTGCAGTCCAACACCATGCATTTAACCACTATTTCCGAAACGTCAAGCAAGGATGGCCTTACAGTCGTTTGCTCAAAAAGAGGAGGTGATGTGTACTTGAATAGTCACTCCAATTGGCTCCAGACGGTCCTTGCTGAACCAGACGCAATCCTCTTTAAGTTTGTACCCATTACTTCTCTTCTTACTGGTCTTCCAGGCAGTGGCTATCTCAGTCATGCAATCAACTTGTATCTACGTTATAAGCCTGATGCAGAGGATTTACAACATTTCTTGGAGTTCCAAGTTCCTAGGCAGTGGGCACCAATGTTTTGTGAGCTTCCTCTTTCACATCAAAGGAAAAGGGCTTCTTGTCCTTCCTTGCAATTCAGTTTCATGGGCCCTAAGATCTTCGTTAGCTCTATGCAGGTATCAACCAATCAAAAACCAGTTGTTGGCCTACGCCTGTATTTAGAAGGAAAAAAGTGCAATAGGTTGGCCATTCATGTGCAGCATCTCTCAAGCCTTCCAAATACTATGGATCTTTCCTTGGCTAACACAAGCACAACAAGACCAAGCCGGTGGCGAGGTTCTGATGATTGTGAGTCGAGTGGCGAATTCTTAGAGCCAATTAAATGGAAAAGATACTCAAGTATCTGTTCATCTGTAGTTGGACATGACCCCAACTGGTTGAAGGGAGATCCAAGTGGTGTGTATATTGTAACCGGTGCACAGCTACTAAGCAAAGGAAAATGGCCAAAGACGATACTTCACCTTCGTCTGCATTATACCTTCCTACCCAATTGTGCAATCCGGAAGACTGAATGGGCTGAGTCGCCAGAGCCTTCTCACAAATCAACATTCCTTACGAATCTCAGCACCACCTTTACGTTCACTCAAAGGACAGCGACTGATCAGCAAAAGCAGGATCCAGCTGCACTTAATTCAGGTGTATATCCAGAAGGCCCCCCAGTGCTAAATCGATCTTCTAAGCTGCTTAAATTTGTGGACACGGCTGAGGTGGTACGAGGGCCACATGATGCTCCGGGGCATTGGTTGGTAACAGCTGCTAAGCTAGTTAAAGAAGGACGTAGAATTGGTCTGCATGTAAAGTTTGCCTTGTTAGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

617

Amino Acids

68.7

Weight (kDa)

9.11

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 125 - 323 4.9e-21 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016822)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12530
malus_domestica MD04G1025600.v1.1
prunus_persica Prupe.1G175000_v2.0.a1
pyrus_communis pycom04g02170
rosa_chinensis RchiOBHm_Chr4g0411351
rosa_laevigata RLG00000008363
rosa_multiflora Rmu_sc0000543.1_g000055
rosa_roxburghii Rroxscaffold_5G00355940
rosa_rugosa Rorug04G0110100
rosa_samantha Rh4AG167300 Rh4CG179000 Rh4DG162100
rosa_wichuraiana Rw4G013830 Rw4G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 978
Acc36I ACCTGC 1 cut(s) 1132
AccB1I GGYRCC 1 cut(s) 1036
AccB7I CCANNNNNTGG 2 cut(s) 938, 1169
AccIII TCCGGA 2 cut(s) 718, 1527
AciI CCGC 2 cut(s) 247, 618
AclWI GGATC 8 cut(s) 246, 494, 507, 651, 1263, 1409, 1637, 1650
AcoI YGGCCR 2 cut(s) 1212, 1469
AcsI RAATTY 5 cut(s) 269, 321, 340, 1331, 1715
AcuI CTGAAG 2 cut(s) 291, 312
AfaI GTAC 5 cut(s) 428, 474, 833, 903, 1740
AfiI CCNNNNNNNGG 2 cut(s) 938, 1169
AgeI ACCGGT 1 cut(s) 1439
AgsI TTSAA 9 cut(s) 94, 142, 253, 269, 274, 314, 374, 838, 1408
AjnI CCWGG 1 cut(s) 931
Alw21I GWGCWC 1 cut(s) 1447
Alw26I GTCTC 1 cut(s) 357
Alw44I GTGCAC 1 cut(s) 1443
AlwI GGATC 8 cut(s) 246, 494, 507, 651, 1263, 1409, 1637, 1650
AlwNI CAGNNNCTG 1 cut(s) 1625
Aor13HI TCCGGA 2 cut(s) 718, 1527
AoxI GGCC 7 cut(s) 793, 1114, 1171, 1212, 1469, 1678, 1745
ApaI GGGCCC 1 cut(s) 1118
ApaLI GTGCAC 1 cut(s) 1443
ApeKI GCWGC 5 cut(s) 506, 1225, 1649, 1708, 1781
ApoI RAATTY 5 cut(s) 269, 321, 340, 1331, 1715
AsiGI ACCGGT 1 cut(s) 1439
AspA2I CCTAGG 1 cut(s) 1025
AspS9I GGNCC 6 cut(s) 671, 865, 1114, 1115, 1679, 1745
AsuC2I CCSGG 1 cut(s) 1761
AsuHPI GGTGA 4 cut(s) 49, 597, 836, 1478
AvaII GGWCC 2 cut(s) 671, 865
AvrII CCTAGG 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 3 cut(s) 1039, 1118, 1447
BaeI ACNNNNGTAYC 4 cut(s) 1351, 1351, 1384, 1384
BalI TGGCCA 2 cut(s) 1214, 1471
BamHI GGATCC 2 cut(s) 499, 1642
BanI GGYRCC 1 cut(s) 1036
BanII GRGCYC 2 cut(s) 46, 1118
BbsI GAAGAC 3 cut(s) 637, 920, 1538
Bbv12I GWGCWC 1 cut(s) 1447
BbvCI CCTCAGC 1 cut(s) 1731
BbvI GCAGC 5 cut(s) 493, 1237, 1636, 1695, 1768
BccI CCATC 5 cut(s) 107, 401, 550, 662, 785
BceAI ACGGC 1 cut(s) 1743
BciT130I CCWGG 1 cut(s) 933
BclI TGATCA 1 cut(s) 1627
BcnI CCSGG 1 cut(s) 1761
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 4 cut(s) 174, 638, 1026, 1790
BfmI CTRYAG 1 cut(s) 1380
BfuAI ACCTGC 1 cut(s) 1132
BglII AGATCT 1 cut(s) 1122
BisI GCNGC 5 cut(s) 507, 1226, 1650, 1709, 1782
BlnI CCTAGG 1 cut(s) 1025
BlpI GCTNAGC 1 cut(s) 1785
BlsI GCNGC 5 cut(s) 508, 1227, 1651, 1710, 1783
Bme1390I CCNGG 2 cut(s) 933, 1761
Bme18I GGWCC 2 cut(s) 671, 865
BmgT120I GGNCC 6 cut(s) 671, 865, 1114, 1115, 1679, 1745
BmiI GGNNCC 6 cut(s) 501, 857, 1038, 1116, 1644, 1681
BmrFI CCNGG 2 cut(s) 933, 1761
BmrI ACTGGG 1 cut(s) 1679
BmsI GCATC 4 cut(s) 455, 976, 1237, 1744
BmuI ACTGGG 1 cut(s) 1679
BpiI GAAGAC 3 cut(s) 637, 920, 1538
BpmI CTGGAG 3 cut(s) 68, 381, 842
Bpu10I CCTNAGC 1 cut(s) 1731
Bpu1102I GCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 2 cut(s) 1219, 1348
BpuMI CCSGG 1 cut(s) 1761
Bsa29I ATCGAT 1 cut(s) 1696
BsaJI CCNNGG 3 cut(s) 1025, 1263, 1760
BsaWI WCCGGW 3 cut(s) 718, 1439, 1527
BsaXI ACNNNNNCTCC 4 cut(s) 433, 463, 813, 843
Bsc4I CCNNNNNNNGG 2 cut(s) 938, 1169
Bse118I RCCGGY 2 cut(s) 1293, 1439
Bse1I ACTGG 7 cut(s) 364, 371, 608, 928, 1163, 1406, 1685
Bse21I CCTNAGG 1 cut(s) 213
BseAI TCCGGA 2 cut(s) 718, 1527
BseBI CCWGG 1 cut(s) 933
BseCI ATCGAT 1 cut(s) 1696
BseDI CCNNGG 3 cut(s) 1025, 1263, 1760
BseGI GGATG 5 cut(s) 12, 118, 522, 561, 796
BseLI CCNNNNNNNGG 2 cut(s) 938, 1169
BseMII CTCAG 9 cut(s) 12, 65, 227, 276, 640, 961, 1536, 1603, 1722
BseNI ACTGG 7 cut(s) 364, 371, 608, 928, 1163, 1406, 1685
BseRI GAGGAG 3 cut(s) 26, 161, 834
BseSI GKGCMC 3 cut(s) 1039, 1118, 1447
BseXI GCAGC 5 cut(s) 493, 1237, 1636, 1695, 1768
BsgI GTGCAG 2 cut(s) 1244, 1635
BshFI GGCC 7 cut(s) 795, 1116, 1173, 1214, 1471, 1680, 1747
BshNI GGYRCC 1 cut(s) 1036
BshTI ACCGGT 1 cut(s) 1439
BshVI ATCGAT 1 cut(s) 1696
BsiHKAI GWGCWC 1 cut(s) 1447
BsiSI CCGG 5 cut(s) 719, 1294, 1440, 1528, 1760
BslFI GGGAC 2 cut(s) 173, 550
BslI CCNNNNNNNGG 2 cut(s) 938, 1169
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 173, 550
BsnI GGCC 7 cut(s) 795, 1116, 1173, 1214, 1471, 1680, 1747
Bsp120I GGGCCC 1 cut(s) 1114
Bsp1286I GDGCHC 4 cut(s) 46, 1039, 1118, 1447
Bsp13I TCCGGA 2 cut(s) 718, 1527
Bsp1407I TGTACA 1 cut(s) 426
Bsp1720I GCTNAGC 1 cut(s) 1785
BspACI CCGC 2 cut(s) 247, 618
BspANI GGCC 7 cut(s) 795, 1116, 1173, 1214, 1471, 1680, 1747
BspCNI CTCAG 9 cut(s) 13, 66, 226, 277, 639, 960, 1537, 1602, 1723
BspDI ATCGAT 1 cut(s) 1696
BspEI TCCGGA 2 cut(s) 718, 1527
BspLI GGNNCC 6 cut(s) 501, 857, 1038, 1116, 1644, 1681
BspMI ACCTGC 1 cut(s) 1132
BspPI GGATC 8 cut(s) 246, 494, 507, 651, 1263, 1409, 1637, 1650
BspT107I GGYRCC 1 cut(s) 1036
BsrFI RCCGGY 2 cut(s) 1293, 1439
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 7 cut(s) 364, 371, 608, 928, 1163, 1406, 1685
BssAI RCCGGY 2 cut(s) 1293, 1439
BssECI CCNNGG 3 cut(s) 1025, 1263, 1760
BssT1I CCWWGG 2 cut(s) 1025, 1263
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 2 cut(s) 802, 865
Bst6I CTCTTC 1 cut(s) 921
BstAUI TGTACA 1 cut(s) 426
BstF5I GGATG 5 cut(s) 12, 118, 522, 561, 796
BstMAI GTCTC 1 cut(s) 357
BstMWI GCNNNNNNNGC 2 cut(s) 50, 792
BstNI CCWGG 1 cut(s) 933
BstNSI RCATGY 1 cut(s) 1823
BstSCI CCNGG 2 cut(s) 931, 1759
BstSFI CTRYAG 1 cut(s) 1380
BstSLI GKGCMC 3 cut(s) 1039, 1118, 1447
BstV1I GCAGC 5 cut(s) 493, 1237, 1636, 1695, 1768
BstV2I GAAGAC 3 cut(s) 637, 920, 1538
BstX2I RGATCY 6 cut(s) 499, 643, 1122, 1255, 1414, 1642
BstXI CCANNNNNNTGG 3 cut(s) 495, 1296, 1350
BstYI RGATCY 6 cut(s) 499, 643, 1122, 1255, 1414, 1642
Bsu15I ATCGAT 1 cut(s) 1696
Bsu36I CCTNAGG 1 cut(s) 213
BsuRI GGCC 7 cut(s) 795, 1116, 1173, 1214, 1471, 1680, 1747
BsuTUI ATCGAT 1 cut(s) 1696
BtsCI GGATG 5 cut(s) 12, 118, 522, 561, 796
BtsI GCAGTG 3 cut(s) 85, 943, 1037
BtsIMutI CAGTG 4 cut(s) 85, 943, 1037, 1692
BveI ACCTGC 1 cut(s) 1132
CaiI CAGNNNCTG 1 cut(s) 1625
Cfr10I RCCGGY 2 cut(s) 1293, 1439
Cfr13I GGNCC 6 cut(s) 671, 865, 1114, 1115, 1679, 1745
ClaI ATCGAT 1 cut(s) 1696
CseI GACGC 1 cut(s) 890
Csp6I GTAC 5 cut(s) 427, 473, 832, 902, 1739
CspAI ACCGGT 1 cut(s) 1439
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 8 cut(s) 203, 754, 953, 1111, 1220, 1391, 1751, 1820
CviQI GTAC 5 cut(s) 427, 473, 832, 902, 1739
EaeI YGGCCR 2 cut(s) 1212, 1469
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
Eco130I CCWWGG 2 cut(s) 1025, 1263
Eco24I GRGCYC 2 cut(s) 46, 1118
Eco32I GATATC 2 cut(s) 335, 379
Eco47I GGWCC 2 cut(s) 671, 865
Eco57I CTGAAG 2 cut(s) 291, 312
Eco81I CCTNAGG 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 1115, 1679
EcoRI GAATTC 3 cut(s) 269, 340, 1331
EcoRII CCWGG 1 cut(s) 931
EcoRV GATATC 2 cut(s) 335, 379
EcoT14I CCWWGG 2 cut(s) 1025, 1263
EcoT22I ATGCAT 1 cut(s) 759
EcoT38I GRGCYC 2 cut(s) 46, 1118
ErhI CCWWGG 2 cut(s) 1025, 1263
FaeI CATG 8 cut(s) 206, 757, 956, 1114, 1223, 1394, 1754, 1823
FalI AAGNNNNNCTT 6 cut(s) 564, 596, 1069, 1101, 1817, 1849
FaqI GGGAC 2 cut(s) 173, 550
FatI CATG 8 cut(s) 202, 753, 952, 1110, 1219, 1390, 1750, 1819
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 1627
Fnu4HI GCNGC 5 cut(s) 507, 1226, 1650, 1709, 1782
FokI GGATG 5 cut(s) 19, 125, 509, 568, 803
FriOI GRGCYC 2 cut(s) 46, 1118
Fsp4HI GCNGC 5 cut(s) 507, 1226, 1650, 1709, 1782
FspBI CTAG 4 cut(s) 174, 638, 1026, 1790
GluI GCNGC 5 cut(s) 507, 1226, 1650, 1709, 1782
GsuI CTGGAG 3 cut(s) 68, 381, 842
HaeIII GGCC 7 cut(s) 795, 1116, 1173, 1214, 1471, 1680, 1747
HapII CCGG 5 cut(s) 719, 1294, 1440, 1528, 1760
HgaI GACGC 1 cut(s) 890
Hin1II CATG 8 cut(s) 206, 757, 956, 1114, 1223, 1394, 1754, 1823
HinfI GANTC 4 cut(s) 243, 1319, 1547, 1585
HpaII CCGG 5 cut(s) 719, 1294, 1440, 1528, 1760
HphI GGTGA 4 cut(s) 49, 597, 836, 1478
Hpy166II GTNNAC 4 cut(s) 832, 1445, 1608, 1723
Hpy8I GTNNAC 4 cut(s) 832, 1445, 1608, 1723
HpyCH4III ACNGT 2 cut(s) 802, 865
HpyCH4IV ACGT 4 cut(s) 779, 973, 1604, 1804
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 792
HpySE526I ACGT 4 cut(s) 779, 973, 1604, 1804
Hsp92II CATG 8 cut(s) 206, 757, 956, 1114, 1223, 1394, 1754, 1823
Kpn2I TCCGGA 2 cut(s) 718, 1527
Ksp22I TGATCA 1 cut(s) 1627
LmnI GCTCC 2 cut(s) 861, 1762
Lsp1109I GCAGC 5 cut(s) 493, 1237, 1636, 1695, 1768
LweI GCATC 4 cut(s) 455, 976, 1237, 1744
MaeI CTAG 4 cut(s) 174, 638, 1026, 1790
MaeII ACGT 4 cut(s) 779, 973, 1604, 1804
MaeIII GTNAC 3 cut(s) 842, 1435, 1774
MboII GAAGA 9 cut(s) 297, 406, 591, 642, 908, 920, 1117, 1543, 1692
MfeI CAATTG 2 cut(s) 850, 1516
MflI RGATCY 6 cut(s) 499, 643, 1122, 1255, 1414, 1642
MhlI GDGCHC 4 cut(s) 46, 1039, 1118, 1447
MlsI TGGCCA 2 cut(s) 1214, 1471
MluNI TGGCCA 2 cut(s) 1214, 1471
MlyI GAGTC 2 cut(s) 1328, 1556
MmeI TCCRAC 3 cut(s) 544, 771, 1366
Mox20I TGGCCA 2 cut(s) 1214, 1471
Mph1103I ATGCAT 1 cut(s) 759
MroI TCCGGA 2 cut(s) 718, 1527
MscI TGGCCA 2 cut(s) 1214, 1471
MseI TTAA 8 cut(s) 294, 356, 761, 894, 1347, 1656, 1713, 1794
MslI CAYNNNNRTG 2 cut(s) 35, 1220
Msp20I TGGCCA 2 cut(s) 1214, 1471
MspA1I CMGCKG 4 cut(s) 506, 620, 1649, 1781
MspI CCGG 5 cut(s) 719, 1294, 1440, 1528, 1760
MspR9I CCNGG 2 cut(s) 933, 1761
MunI CAATTG 2 cut(s) 850, 1516
MvaI CCWGG 1 cut(s) 933
MwoI GCNNNNNNNGC 2 cut(s) 50, 792
NciI CCSGG 1 cut(s) 1761
NdeI CATATG 1 cut(s) 532
NlaIII CATG 8 cut(s) 206, 757, 956, 1114, 1223, 1394, 1754, 1823
NlaIV GGNNCC 6 cut(s) 501, 857, 1038, 1116, 1644, 1681
NmuCI GTSAC 1 cut(s) 842
NsiI ATGCAT 1 cut(s) 759
NspI RCATGY 1 cut(s) 1823
PfeI GAWTC 2 cut(s) 243, 1585
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 2 cut(s) 938, 1169
PinAI ACCGGT 1 cut(s) 1439
PkrI GCNGC 5 cut(s) 508, 1227, 1651, 1710, 1783
PleI GAGTC 2 cut(s) 1327, 1555
PpsI GAGTC 2 cut(s) 1327, 1555
PsiI TTATAA 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 931
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 6 cut(s) 501, 857, 1038, 1116, 1644, 1681
PspOMI GGGCCC 1 cut(s) 1114
PspPI GGNCC 6 cut(s) 671, 865, 1114, 1115, 1679, 1745
PstNI CAGNNNCTG 1 cut(s) 1625
PsuI RGATCY 6 cut(s) 499, 643, 1122, 1255, 1414, 1642
PsyI GACNNNGTC 1 cut(s) 164
PvuII CAGCTG 3 cut(s) 506, 1649, 1781
RsaI GTAC 5 cut(s) 428, 474, 833, 903, 1740
RsaNI GTAC 5 cut(s) 427, 473, 832, 902, 1739
RseI CAYNNNNRTG 2 cut(s) 35, 1220
SaqAI TTAA 8 cut(s) 294, 356, 761, 894, 1347, 1656, 1713, 1794
SatI GCNGC 5 cut(s) 507, 1226, 1650, 1709, 1782
Sau96I GGNCC 6 cut(s) 671, 865, 1114, 1115, 1679, 1745
SchI GAGTC 2 cut(s) 1328, 1556
ScrFI CCNGG 2 cut(s) 933, 1761
SduI GDGCHC 4 cut(s) 46, 1039, 1118, 1447
SfaNI GCATC 4 cut(s) 455, 976, 1237, 1744
SfcI CTRYAG 1 cut(s) 1380
SinI GGWCC 2 cut(s) 671, 865
SmiMI CAYNNNNRTG 2 cut(s) 35, 1220
SmlI CTYRAG 2 cut(s) 1234, 1363
SmoI CTYRAG 2 cut(s) 1234, 1363
SsiI CCGC 2 cut(s) 247, 618
SspI AATATT 1 cut(s) 715
SspMI CTAG 4 cut(s) 174, 638, 1026, 1790
StyD4I CCNGG 2 cut(s) 931, 1759
StyI CCWWGG 2 cut(s) 1025, 1263
TaaI ACNGT 2 cut(s) 802, 865
TaiI ACGT 4 cut(s) 782, 976, 1607, 1807
TaqI TCGA 4 cut(s) 241, 515, 1322, 1696
TatI WGTACW 3 cut(s) 426, 472, 831
TfiI GAWTC 2 cut(s) 243, 1585
Tru1I TTAA 8 cut(s) 294, 356, 761, 894, 1347, 1656, 1713, 1794
Tru9I TTAA 8 cut(s) 294, 356, 761, 894, 1347, 1656, 1713, 1794
TscAI CASTG 4 cut(s) 85, 943, 1037, 1692
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 5 cut(s) 506, 1225, 1649, 1708, 1781
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 9 cut(s) 191, 212, 406, 411, 511, 519, 1099, 1208, 1365
TspGWI ACGGA 1 cut(s) 515
TspRI CASTG 4 cut(s) 85, 943, 1037, 1692
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 2 cut(s) 938, 1169
VneI GTGCAC 1 cut(s) 1443
VpaK11BI GGWCC 2 cut(s) 671, 865
XapI RAATTY 5 cut(s) 269, 321, 340, 1331, 1715
XceI RCATGY 1 cut(s) 1823
XmaJI CCTAGG 1 cut(s) 1025
XspI CTAG 4 cut(s) 174, 638, 1026, 1790
Zsp2I ATGCAT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.