MD04G1025600.v1.1

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016822
Physical Location & Seq
Forward (+)
2963765 .. 2969764
6000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1025600.v1.1.491

Sequence Viewer

Length: 1845 bp
ATGAAGATGGAGGGTGCCCCCCAGAAGGCCATAGAAGTGAGGGCGTTGGAGGCACTTGGGCAGGGATTCGATTTGAGCAGCGATTTCAGGCTCAAGTTTGCGAAAGGGTTGAAGGGAGGTGATGGGAGGTTGGTAGTGCTGAATGAGGCTAGTAAGAGGGACATTGTGATTCCAAATGCTGGAGGAGTTGTCATTCGTGGAGTTCCTCAGGATATTAGTTGCGATAAAGGCGATCGAATTCGGTTCAAATCCGATGTTCTTGAATTAAATCAGATGTCGGAGCTACTTAATCAGAAATCTTCTGTGCAAGGGAAAATTCCTTCAGGCTATCTTAATGCCATTTTCGATTTAACTGGAGACTGGCTTCAAGATGCTGCAGAAACCAAATCTCTTGCTTTCGATGGTTTCTTCATTTCATTATACTATCTGCAACTAACAGCATCTCCATTAATATTGCATAACAAAGTACAGAAATCTGTTCCATCTCGTTGGGATCCAGCCGCATTATCTAGGTTCATCCGTTCATATGGGACACACATAATAATAGGAATGGCTGTTGGAGGTCAAGATTTAGTTTGTGTCAAACAGAAATATTCCTCACCGGTTCCCACAGCTGATGTCAGAAGACGCCTGGAGGATCTTGGGGATTTCCTATTTTCAGGCGGAAGCCCTTCTTTACTACGGGGAAAAACAAGAGATGGACGACATAAAGTTCCAGATGTATTCGATCCTATTTTGCAGACAAACACTATGCAGTTAACCACTTTTCCCGAAACATCAAGCAAGGATGGTCTTACTATGATTTTCTCAAAACGAGGAGGGGATGTGTTCTCGAATGGTCATTCCAATTGGCTCCAGTCAGTGCTTACTGAACCAGAAGCTATCCTCTTTAAGTTTGTACCTATTACTTCTCTTCTTACTGGGATTCCAGGAAGTGGCTATCTTAGTCATGCAATCAACTTGTATCTACGTTACAAGCCTGCTCCAGAGGATTTACAACATTTCTTGGAGTTCCAAGTTCCTAGGCAATGGGCACCTATGTTCTGTGAGATGCCTCTATCGCTTCAACGTAAAAAGGCCACCTGTCCTACCTTGCAATTCAGTTTAATGGGTCCTAAGATCTATGTTAACTCTACACAGGTTTCAACTAGTCAAAAGCCAGTTGTTGGCCTACGCCTGTACTTAGAAGGGAAAAGACGAAATCGGCTAGCCCTACATGTACAACATCTCTCGAGCCTTCCAAATACTATGGATCTTTCAGTGGGTAGTACCAGCACAAGGCCAAGCTGGTGGCGGGGTTCTGACGACTCTGAGTTGAGCAACCAGTTCTTAGAGCCAATTAATTGGAAGAGATATTCGAAAATTTGTTCGTCTGTAGTCAAGCATGACCCCAACTGGTTGAAGGGAGATTCAGATGGCGTGTACATTGTAACTGGTGCACAGCTCCTTAGCAAAGGGAAATGGCCCAAGACAGTACTTCACCTTCGTCTGCATTTTTCCCACGTGCCCAATTGTGGAATCCGGAAAACTGAGTGGGCTGCGGTACCAGAGGCTTCTCACAAATCAAGTTTCCTTACGAATCTGAGCACAACGTTCACCTTCACTCAGCGGACAGTAACTGATCAGCAAAAACAGGATCCAGCTGCACTTAATTCGGGTGTATATCCAGATGGACCACCCATGCTAAATCACTCTTCAAAGCTTCTTAAATACGTGGAAACAGCGGAGGTTGTCCGTGGCCCGCATGATGCTCCGGGACATTGGTTAGTAACAGCTGCTAAACTAGTAAAAGAGGGAGGTAAAATTGGTTTACATGTAAAGTTTGCCTTGTTAGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

615

Amino Acids

68.23

Weight (kDa)

9.41

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 118 - 322 2.9e-22 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016822)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12530
malus_domestica MD04G1025600.v1.1
prunus_persica Prupe.1G175000_v2.0.a1
pyrus_communis pycom04g02170
rosa_chinensis RchiOBHm_Chr4g0411351
rosa_laevigata RLG00000008363
rosa_multiflora Rmu_sc0000543.1_g000055
rosa_roxburghii Rroxscaffold_5G00355940
rosa_rugosa Rorug04G0110100
rosa_samantha Rh4AG167300 Rh4CG179000 Rh4DG162100
rosa_wichuraiana Rw4G013830 Rw4G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1543
AccB1I GGYRCC 3 cut(s) 14, 1033, 1543
AccB7I CCANNNNNTGG 3 cut(s) 179, 935, 1166
AccIII TCCGGA 1 cut(s) 1521
AciI CCGC 7 cut(s) 501, 663, 1294, 1541, 1609, 1724, 1742
AclI AACGTT 1 cut(s) 1592
AclWI GGATC 7 cut(s) 488, 501, 645, 722, 1260, 1631, 1644
AcsI RAATTY 3 cut(s) 237, 315, 1362
AcuI CTGAAG 1 cut(s) 306
AcvI CACGTG 1 cut(s) 1504
AcyI GRCGYC 1 cut(s) 628
AfaI GTAC 8 cut(s) 468, 900, 1181, 1221, 1270, 1424, 1476, 1545
AfiI CCNNNNNNNGG 6 cut(s) 25, 179, 935, 1166, 1278, 1514
AflIII ACRYGT 2 cut(s) 1216, 1813
AgeI ACCGGT 1 cut(s) 601
AgsI TTSAA 8 cut(s) 112, 247, 263, 368, 1067, 1146, 1402, 1698
AhlI ACTAGT 2 cut(s) 1148, 1783
AjnI CCWGG 2 cut(s) 630, 928
AluBI AGCT 8 cut(s) 283, 614, 881, 1287, 1444, 1643, 1702, 1775
AluI AGCT 8 cut(s) 283, 614, 881, 1287, 1444, 1643, 1702, 1775
Alw21I GWGCWC 2 cut(s) 1441, 1589
Alw26I GTCTC 1 cut(s) 351
Alw44I GTGCAC 1 cut(s) 1437
AlwI GGATC 7 cut(s) 488, 501, 645, 722, 1260, 1631, 1644
AlwNI CAGNNNCTG 1 cut(s) 1619
Ama87I CYCGRG 1 cut(s) 1231
Aor13HI TCCGGA 1 cut(s) 1521
AoxI GGCC 6 cut(s) 27, 1077, 1168, 1280, 1463, 1738
ApaLI GTGCAC 1 cut(s) 1437
ApeKI GCWGC 5 cut(s) 78, 374, 1538, 1643, 1775
ApoI RAATTY 3 cut(s) 237, 315, 1362
AseI ATTAAT 2 cut(s) 449, 1341
AsiGI ACCGGT 1 cut(s) 601
Asp700I GAANNNNTTC 1 cut(s) 670
Asp718I GGTACC 1 cut(s) 1543
AspA2I CCTAGG 1 cut(s) 1022
AspS9I GGNCC 4 cut(s) 1112, 1464, 1673, 1739
AsuC2I CCSGG 1 cut(s) 1755
AsuHPI GGTGA 4 cut(s) 131, 591, 1472, 1588
AsuII TTCGAA 1 cut(s) 1358
AsuNHI GCTAGC 1 cut(s) 1207
AvaI CYCGRG 1 cut(s) 1231
AvaII GGWCC 2 cut(s) 1112, 1673
AvrII CCTAGG 1 cut(s) 1022
AxyI CCTNAGG 1 cut(s) 207
BaeGI GKGCMC 4 cut(s) 19, 1036, 1441, 1509
BamHI GGATCC 2 cut(s) 493, 1636
BanI GGYRCC 3 cut(s) 14, 1033, 1543
BarI GAAGNNNNNNTAC 2 cut(s) 1467, 1499
BbrPI CACGTG 1 cut(s) 1504
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 2 cut(s) 1441, 1589
BbvI GCAGC 5 cut(s) 90, 361, 1525, 1630, 1762
BccI CCATC 7 cut(s) 116, 395, 490, 692, 782, 1409, 1664
BcgI CGANNNNNNTGC 2 cut(s) 1635, 1669
BciT130I CCWGG 2 cut(s) 632, 930
BclI TGATCA 1 cut(s) 1621
BcnI CCSGG 1 cut(s) 1755
BcoDI GTCTC 1 cut(s) 351
BcuI ACTAGT 2 cut(s) 1148, 1783
BfaI CTAG 6 cut(s) 150, 510, 1023, 1149, 1208, 1784
BfmI CTRYAG 2 cut(s) 375, 1374
BglII AGATCT 1 cut(s) 1119
BisI GCNGC 6 cut(s) 79, 375, 501, 1539, 1644, 1776
BlnI CCTAGG 1 cut(s) 1022
BlsI GCNGC 6 cut(s) 80, 376, 502, 1540, 1645, 1777
BmcAI AGTACT 1 cut(s) 1476
Bme1390I CCNGG 3 cut(s) 632, 930, 1755
Bme18I GGWCC 2 cut(s) 1112, 1673
BmeT110I CYCGRG 1 cut(s) 1231
BmgT120I GGNCC 4 cut(s) 1112, 1464, 1673, 1739
BmiI GGNNCC 8 cut(s) 16, 495, 606, 854, 1035, 1113, 1545, 1638
BmrFI CCNGG 3 cut(s) 632, 930, 1755
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 4 cut(s) 361, 449, 1041, 1738
BmtI GCTAGC 1 cut(s) 1211
BmuI ACTGGG 1 cut(s) 930
BpiI GAAGAC 1 cut(s) 631
BpmI CTGGAG 5 cut(s) 201, 375, 653, 839, 969
Bpu10I CCTNAGC 1 cut(s) 1448
Bpu14I TTCGAA 1 cut(s) 1358
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 1755
BsaAI YACGTR 2 cut(s) 1504, 1714
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 1022, 1735
BsaWI WCCGGW 2 cut(s) 601, 1521
BsaXI ACNNNNNCTCC 6 cut(s) 174, 204, 427, 457, 810, 840
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 179, 935, 1166, 1278, 1514
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 8 cut(s) 358, 365, 856, 925, 1160, 1324, 1400, 1438
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 1034
BseAI TCCGGA 1 cut(s) 1521
BseBI CCWGG 2 cut(s) 632, 930
BseDI CCNNGG 2 cut(s) 1022, 1735
BseGI GGATG 3 cut(s) 516, 793, 829
BseLI CCNNNNNNNGG 6 cut(s) 25, 179, 935, 1166, 1278, 1514
BseMI GCAATG 1 cut(s) 1034
BseMII CTCAG 5 cut(s) 221, 1302, 1521, 1574, 1619
BseNI ACTGG 8 cut(s) 358, 365, 856, 925, 1160, 1324, 1400, 1438
BseRI GAGGAG 2 cut(s) 198, 831
BseSI GKGCMC 4 cut(s) 19, 1036, 1441, 1509
BseXI GCAGC 5 cut(s) 90, 361, 1525, 1630, 1762
BsgI GTGCAG 1 cut(s) 1629
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 6 cut(s) 29, 1079, 1170, 1282, 1465, 1740
BshNI GGYRCC 3 cut(s) 14, 1033, 1543
BshTI ACCGGT 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 1441, 1589
BsiHKCI CYCGRG 1 cut(s) 1231
BsiSI CCGG 3 cut(s) 602, 1522, 1754
BslFI GGGAC 3 cut(s) 173, 544, 1770
BslI CCNNNNNNNGG 6 cut(s) 25, 179, 935, 1166, 1278, 1514
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 3 cut(s) 173, 544, 1770
BsnI GGCC 6 cut(s) 29, 1079, 1170, 1282, 1465, 1740
BsoBI CYCGRG 1 cut(s) 1231
Bsp119I TTCGAA 1 cut(s) 1358
Bsp1286I GDGCHC 5 cut(s) 19, 1036, 1441, 1509, 1589
Bsp13I TCCGGA 1 cut(s) 1521
Bsp1407I TGTACA 2 cut(s) 1219, 1422
Bsp143I GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
BspACI CCGC 7 cut(s) 501, 663, 1294, 1541, 1609, 1724, 1742
BspANI GGCC 6 cut(s) 29, 1079, 1170, 1282, 1465, 1740
BspCNI CTCAG 5 cut(s) 220, 1303, 1522, 1575, 1618
BspEI TCCGGA 1 cut(s) 1521
BspLI GGNNCC 8 cut(s) 16, 495, 606, 854, 1035, 1113, 1545, 1638
BspMAI CTGCAG 1 cut(s) 379
BspOI GCTAGC 1 cut(s) 1211
BspPI GGATC 7 cut(s) 488, 501, 645, 722, 1260, 1631, 1644
BspT104I TTCGAA 1 cut(s) 1358
BspT107I GGYRCC 3 cut(s) 14, 1033, 1543
BsrDI GCAATG 1 cut(s) 1034
BsrFI RCCGGY 1 cut(s) 601
BsrGI TGTACA 2 cut(s) 1219, 1422
BsrI ACTGG 8 cut(s) 358, 365, 856, 925, 1160, 1324, 1400, 1438
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 1022, 1735
BssMI GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
BssNI GRCGYC 1 cut(s) 628
BssT1I CCWWGG 1 cut(s) 1022
Bst2UI CCWGG 2 cut(s) 632, 930
Bst4CI ACNGT 2 cut(s) 1474, 1615
Bst6I CTCTTC 3 cut(s) 918, 1343, 1699
BstACI GRCGYC 1 cut(s) 628
BstAUI TGTACA 2 cut(s) 1219, 1422
BstBAI YACGTR 2 cut(s) 1504, 1714
BstBI TTCGAA 1 cut(s) 1358
BstC8I GCNNGC 3 cut(s) 981, 1209, 1742
BstDSI CCRYGG 1 cut(s) 1735
BstF5I GGATG 3 cut(s) 516, 793, 829
BstKTI GATC 8 cut(s) 235, 496, 640, 730, 1122, 1255, 1624, 1639
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 50, 228, 1060
BstNI CCWGG 2 cut(s) 632, 930
BstNSI RCATGY 2 cut(s) 1220, 1817
BstSCI CCNGG 3 cut(s) 630, 928, 1753
BstSFI CTRYAG 2 cut(s) 375, 1374
BstSLI GKGCMC 4 cut(s) 19, 1036, 1441, 1509
BstV1I GCAGC 5 cut(s) 90, 361, 1525, 1630, 1762
BstV2I GAAGAC 1 cut(s) 631
BstX2I RGATCY 5 cut(s) 493, 637, 1119, 1252, 1636
BstXI CCANNNNNNTGG 3 cut(s) 489, 1290, 1344
BstYI RGATCY 5 cut(s) 493, 637, 1119, 1252, 1636
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 6 cut(s) 29, 1079, 1170, 1282, 1465, 1740
BtgI CCRYGG 1 cut(s) 1735
BtsCI GGATG 3 cut(s) 516, 793, 829
BtsIMutI CAGTG 2 cut(s) 867, 1266
Cac8I GCNNGC 3 cut(s) 981, 1209, 1742
CaiI CAGNNNCTG 1 cut(s) 1619
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 4 cut(s) 1112, 1464, 1673, 1739
CseI GACGC 1 cut(s) 636
Csp6I GTAC 8 cut(s) 467, 899, 1180, 1220, 1269, 1423, 1475, 1544
CspAI ACCGGT 1 cut(s) 601
CviAII CATG 6 cut(s) 950, 1217, 1385, 1681, 1745, 1814
CviQI GTAC 8 cut(s) 467, 899, 1180, 1220, 1269, 1423, 1475, 1544
DpnI GATC 8 cut(s) 234, 495, 639, 729, 1121, 1254, 1623, 1638
DpnII GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
Eam1104I CTCTTC 3 cut(s) 918, 1343, 1699
EarI CTCTTC 3 cut(s) 918, 1343, 1699
EciI GGCGGA 1 cut(s) 678
Eco130I CCWWGG 1 cut(s) 1022
Eco47I GGWCC 2 cut(s) 1112, 1673
Eco57I CTGAAG 1 cut(s) 306
Eco72I CACGTG 1 cut(s) 1504
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 1231
EcoO109I RGGNCCY 1 cut(s) 1112
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 630, 928
EcoT14I CCWWGG 1 cut(s) 1022
ErhI CCWWGG 1 cut(s) 1022
FaeI CATG 6 cut(s) 953, 1220, 1388, 1684, 1748, 1817
FalI AAGNNNNNCTT 4 cut(s) 658, 690, 1811, 1843
FaqI GGGAC 3 cut(s) 173, 544, 1770
FatI CATG 6 cut(s) 949, 1216, 1384, 1680, 1744, 1813
FauI CCCGC 2 cut(s) 1287, 1749
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 1 cut(s) 1621
Fnu4HI GCNGC 6 cut(s) 79, 375, 501, 1539, 1644, 1776
FokI GGATG 3 cut(s) 503, 800, 836
Fsp4HI GCNGC 6 cut(s) 79, 375, 501, 1539, 1644, 1776
FspBI CTAG 6 cut(s) 150, 510, 1023, 1149, 1208, 1784
GluI GCNGC 6 cut(s) 79, 375, 501, 1539, 1644, 1776
GsuI CTGGAG 5 cut(s) 201, 375, 653, 839, 969
HaeIII GGCC 6 cut(s) 29, 1079, 1170, 1282, 1465, 1740
HapII CCGG 3 cut(s) 602, 1522, 1754
HgaI GACGC 1 cut(s) 636
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 6 cut(s) 953, 1220, 1388, 1684, 1748, 1817
HincII GTYRAC 2 cut(s) 759, 1129
HindII GTYRAC 2 cut(s) 759, 1129
HindIII AAGCTT 1 cut(s) 1700
HinfI GANTC 7 cut(s) 66, 169, 925, 1307, 1409, 1518, 1579
HpaI GTTAAC 2 cut(s) 759, 1129
HpaII CCGG 3 cut(s) 602, 1522, 1754
HphI GGTGA 4 cut(s) 131, 591, 1472, 1588
Hpy166II GTNNAC 6 cut(s) 759, 1129, 1423, 1439, 1596, 1811
Hpy188I TCNGA 9 cut(s) 253, 273, 280, 294, 623, 1303, 1312, 1414, 1584
Hpy8I GTNNAC 6 cut(s) 759, 1129, 1423, 1439, 1596, 1811
HpyAV CCTTC 9 cut(s) 19, 106, 330, 681, 1181, 1247, 1396, 1493, 1609
HpyCH4III ACNGT 2 cut(s) 1474, 1615
HpyCH4IV ACGT 5 cut(s) 970, 1069, 1503, 1592, 1713
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 228, 1060
HpySE526I ACGT 5 cut(s) 970, 1069, 1503, 1592, 1713
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 6 cut(s) 953, 1220, 1388, 1684, 1748, 1817
Kpn2I TCCGGA 1 cut(s) 1521
KpnI GGTACC 1 cut(s) 1547
Ksp22I TGATCA 1 cut(s) 1621
KspAI GTTAAC 2 cut(s) 759, 1129
Kzo9I GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
LmnI GCTCC 5 cut(s) 280, 858, 988, 1449, 1756
Lsp1109I GCAGC 5 cut(s) 90, 361, 1525, 1630, 1762
LweI GCATC 4 cut(s) 361, 449, 1041, 1738
MaeI CTAG 6 cut(s) 150, 510, 1023, 1149, 1208, 1784
MaeII ACGT 5 cut(s) 970, 1069, 1503, 1592, 1713
MaeIII GTNAC 4 cut(s) 971, 1429, 1615, 1768
MalI GATC 8 cut(s) 234, 495, 639, 729, 1121, 1254, 1623, 1638
MboI GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
MboII GAAGA 7 cut(s) 16, 291, 400, 636, 905, 1360, 1686
MfeI CAATTG 2 cut(s) 847, 1510
MflI RGATCY 5 cut(s) 493, 637, 1119, 1252, 1636
MhlI GDGCHC 5 cut(s) 19, 1036, 1441, 1509, 1589
MlyI GAGTC 1 cut(s) 1301
MmeI TCCRAC 3 cut(s) 27, 258, 538
MroI TCCGGA 1 cut(s) 1521
MroXI GAANNNNTTC 1 cut(s) 670
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 5 cut(s) 614, 1609, 1643, 1724, 1775
MspI CCGG 3 cut(s) 602, 1522, 1754
MspR9I CCNGG 3 cut(s) 632, 930, 1755
MunI CAATTG 2 cut(s) 847, 1510
MvaI CCWGG 2 cut(s) 632, 930
MwoI GCNNNNNNNGC 3 cut(s) 50, 228, 1060
NciI CCSGG 1 cut(s) 1755
NdeI CATATG 1 cut(s) 526
NdeII GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
NheI GCTAGC 1 cut(s) 1207
NlaIII CATG 6 cut(s) 953, 1220, 1388, 1684, 1748, 1817
NlaIV GGNNCC 8 cut(s) 16, 495, 606, 854, 1035, 1113, 1545, 1638
NspI RCATGY 2 cut(s) 1220, 1817
NspV TTCGAA 1 cut(s) 1358
PaeR7I CTCGAG 1 cut(s) 1231
PciI ACATGT 2 cut(s) 1216, 1813
PdmI GAANNNNTTC 1 cut(s) 670
PfeI GAWTC 6 cut(s) 66, 169, 925, 1409, 1518, 1579
PflMI CCANNNNNTGG 3 cut(s) 179, 935, 1166
PfoI TCCNGGA 2 cut(s) 928, 1753
PinAI ACCGGT 1 cut(s) 601
PkrI GCNGC 6 cut(s) 80, 376, 502, 1540, 1645, 1777
Ple19I CGATCG 1 cut(s) 235
PleI GAGTC 1 cut(s) 1301
PmaCI CACGTG 1 cut(s) 1504
PmlI CACGTG 1 cut(s) 1504
PpsI GAGTC 1 cut(s) 1301
Ppu21I YACGTR 2 cut(s) 1504, 1714
PpuMI RGGWCCY 1 cut(s) 1112
PscI ACATGT 2 cut(s) 1216, 1813
PshBI ATTAAT 2 cut(s) 449, 1341
Psp1406I AACGTT 1 cut(s) 1592
Psp5II RGGWCCY 1 cut(s) 1112
Psp6I CCWGG 2 cut(s) 630, 928
PspCI CACGTG 1 cut(s) 1504
PspGI CCWGG 2 cut(s) 630, 928
PspN4I GGNNCC 8 cut(s) 16, 495, 606, 854, 1035, 1113, 1545, 1638
PspPI GGNCC 4 cut(s) 1112, 1464, 1673, 1739
PspPPI RGGWCCY 1 cut(s) 1112
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 1619
PsuI RGATCY 5 cut(s) 493, 637, 1119, 1252, 1636
PvuI CGATCG 1 cut(s) 235
PvuII CAGCTG 3 cut(s) 614, 1643, 1775
RsaI GTAC 8 cut(s) 468, 900, 1181, 1221, 1270, 1424, 1476, 1545
RsaNI GTAC 8 cut(s) 467, 899, 1180, 1220, 1269, 1423, 1475, 1544
RseI CAYNNNNRTG 1 cut(s) 35
SatI GCNGC 6 cut(s) 79, 375, 501, 1539, 1644, 1776
Sau3AI GATC 8 cut(s) 232, 493, 637, 727, 1119, 1252, 1621, 1636
Sau96I GGNCC 4 cut(s) 1112, 1464, 1673, 1739
ScaI AGTACT 1 cut(s) 1476
SchI GAGTC 1 cut(s) 1301
ScrFI CCNGG 3 cut(s) 632, 930, 1755
SduI GDGCHC 5 cut(s) 19, 1036, 1441, 1509, 1589
SfaNI GCATC 4 cut(s) 361, 449, 1041, 1738
SfcI CTRYAG 2 cut(s) 375, 1374
Sfr274I CTCGAG 1 cut(s) 1231
SfuI TTCGAA 1 cut(s) 1358
SinI GGWCC 2 cut(s) 1112, 1673
SlaI CTCGAG 1 cut(s) 1231
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 92, 1231
SmoI CTYRAG 2 cut(s) 92, 1231
SpeI ACTAGT 2 cut(s) 1148, 1783
SsiI CCGC 7 cut(s) 501, 663, 1294, 1541, 1609, 1724, 1742
SspI AATATT 2 cut(s) 453, 593
SspMI CTAG 6 cut(s) 150, 510, 1023, 1149, 1208, 1784
StyD4I CCNGG 3 cut(s) 630, 928, 1753
StyI CCWWGG 1 cut(s) 1022
TaaI ACNGT 2 cut(s) 1474, 1615
TaiI ACGT 5 cut(s) 973, 1072, 1506, 1595, 1716
TaqI TCGA 8 cut(s) 69, 235, 345, 399, 726, 833, 1232, 1358
TatI WGTACW 5 cut(s) 466, 1179, 1219, 1422, 1474
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 6 cut(s) 66, 169, 925, 1409, 1518, 1579
TscAI CASTG 2 cut(s) 867, 1266
TseI GCWGC 5 cut(s) 78, 374, 1538, 1643, 1775
TspDTI ATGAA 5 cut(s) 17, 400, 405, 505, 513
TspGWI ACGGA 2 cut(s) 509, 1724
TspRI CASTG 2 cut(s) 867, 1266
Van91I CCANNNNNTGG 3 cut(s) 179, 935, 1166
VneI GTGCAC 1 cut(s) 1437
VpaK11BI GGWCC 2 cut(s) 1112, 1673
VspI ATTAAT 2 cut(s) 449, 1341
XapI RAATTY 3 cut(s) 237, 315, 1362
XceI RCATGY 2 cut(s) 1220, 1817
XhoI CTCGAG 1 cut(s) 1231
XmaJI CCTAGG 1 cut(s) 1022
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 6 cut(s) 150, 510, 1023, 1149, 1208, 1784
ZrmI AGTACT 1 cut(s) 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.