Rw4G013830

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016822
Physical Location & Seq
Reverse (-)
32350715 .. 32358231
7517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013830.1

Sequence Viewer

Length: 1839 bp
ATGAGGATGGAAGAGGGTGCTGAGAAGCCCATAGAGGTGAGGGCTTTAGAGGCGCTTGGGCAGGGGTTTGATTTGAGCAGTGATTTCAGGTTGAAGTTTGCAAAAGGGTTGGATGGGAATAAGGGGAGGTTGGTGGTTCTGAATGAGGATAGTAAGAGGGACATTGTCATTCCTAGTGGTAGTGGTGGGGGAGTTGTCATTCATGGAGTTCCTCAGGATATTCACTGCGACAAAGGGGATCGAATCCGCTTCAAATCCGATGTTCTTGAATTCAATCAGATGTCTGAGCTACTTAATCAGAAATCTTCAGTTCAAGGGAAAATTCCTTCAGGATATCTGAATTCCATTTTTGATTTAACTGGAGACTGGCTTCAAGATGCTACAGAAACAAAATATCTTGCTTTTGATGGTTTCTTCATTTCACTGTACAATTTGCACCTAACAGCATCTCCACTAATTTTGCGAAACAAAGTACAGAAATCTGTTCCATCTCGTTGGGATCCAGCTGCATTGTCTAGGTTCATCCATTCATACGGAACACACATAATAGTTGGGATGGCAGTTGGAGGTCAAGATTTAGTTTGTGTTAGGCAGAAATCTTCCTCACCAGTTCCTCCCGCTGATCTCAGAAGACACTTGGAGGATCTTGGAGATTTTCTATTTTCAGATGGACCTTCTTTACTACGGGGAAAAACAAGAAATGGAATACAAAAAATTCCGGAGGTGTTCGATCGTATTTTGCAGTCCAACTCCATGCATTTATCCACTATTTCCGAAACGTCAAGCAAGGATGGCCTTACAGTCGTTTGCTCGAAAAGAGGCGGTGATGTGTTTCTGAATAGTCACTCCAATTGGCTCCAGACAGTCCTTGTTGAACCAGAAGCAATCCTCTTTAAGTTTGTACCCATTACTTCTCTTCTTACTGGTCTTCCAGGCAGTGGTATCTCAGTCATGCAATCAACTTATAAGCCTGATGCAGAGGATTTACAACATTTCTTGGAGTTCCAAGTCCCGAGGCAATGGGCGCCAATGTTTTGTGAGCTTCCTCTTTCTCATCAACGGAAAAGGGCTTCTTGTCCTTCCCTGCAATTCAGTTTCATGGGCCCTAAGATCTTTGTTAGCTCTAAGCAGGTTTCAACCAGTCAAAAACCAATTGTTGGCCTACGCCTGTATTTAGAAGGAAAAAAGTGCAATCGGTTGGCCGTTCATGTGCAGCATCTCTCAAGCCTTCCAAATACTATGGAACTTTCTTTGGCAAACACAAGCACAACAAGACCAAGCCGGTGGCGAGGTTCTGATGATTGTGAGCTGAGTGGTGAATTCTTAGAGCCGATTAAATGGAAAAGATACTCAAGCATCTGTTCATCTGTAGTTGGACACGACCCCAACTGGTTGAAGGGAGGTCCAAGTGGTGTGTATATTGTAACTGGTGCACAACTACTAAGCAAAGGAAAATGGCCAAAGACGGTACTTCACCTTCGTCTGCATTACACCTTTCTACCCAATTGTGCAATCCGGAAGACTGAATGGGCTGGGTCACCGGAGCCTTCTCACAAATCAACGTTCCTTACAAATCTCAGCACCACGTTTACGTTCACTCAAAGGACAGTTACTGATCAGCAAAAGCAGGATCCAGCTGCACTTAATTCAGGTGTATATCCAGAAGGCCCTCCAGTGCTAAATCAATCTTCTAAGCTGCTAAAATTTGTGGACACGGCTGAGGTGGTGCGAGGGCCACATGATGCTCCAGGGCATTGGTTAGTAACAGCTGCTAAGCTAGTAAAAGAGGGACGTAGAATTGGTCTGCATGTAAAGTTTGCCTTGTTAGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

612

Amino Acids

67.95

Weight (kDa)

9.12

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 123 - 311 1.9e-17 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016822)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12530
malus_domestica MD04G1025600.v1.1
prunus_persica Prupe.1G175000_v2.0.a1
pyrus_communis pycom04g02170
rosa_chinensis RchiOBHm_Chr4g0411351
rosa_laevigata RLG00000008363
rosa_multiflora Rmu_sc0000543.1_g000055
rosa_roxburghii Rroxscaffold_5G00355940
rosa_rugosa Rorug04G0110100
rosa_samantha Rh4AG167300 Rh4CG179000 Rh4DG162100
rosa_wichuraiana Rw4G013830 Rw4G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 966
Acc36I ACCTGC 1 cut(s) 1120
AccB1I GGYRCC 1 cut(s) 1024
AccB7I CCANNNNNTGG 2 cut(s) 938, 1157
AccIII TCCGGA 2 cut(s) 718, 1515
AciI CCGC 3 cut(s) 247, 618, 822
AclI AACGTT 1 cut(s) 1562
AclWI GGATC 6 cut(s) 246, 494, 507, 651, 1625, 1638
AcoI YGGCCR 2 cut(s) 1200, 1457
AcsI RAATTY 6 cut(s) 269, 321, 340, 714, 1319, 1703
AcuI CTGAAG 2 cut(s) 291, 312
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 4 cut(s) 428, 474, 903, 1470
AfiI CCNNNNNNNGG 2 cut(s) 938, 1157
AgsI TTSAA 9 cut(s) 94, 253, 269, 274, 314, 374, 875, 1137, 1396
AjnI CCWGG 2 cut(s) 931, 1747
AluBI AGCT 9 cut(s) 289, 506, 1042, 1122, 1309, 1637, 1696, 1769, 1777
AluI AGCT 9 cut(s) 289, 506, 1042, 1122, 1309, 1637, 1696, 1769, 1777
Alw21I GWGCWC 1 cut(s) 1435
Alw26I GTCTC 1 cut(s) 357
Alw44I GTGCAC 1 cut(s) 1431
AlwI GGATC 6 cut(s) 246, 494, 507, 651, 1625, 1638
AlwNI CAGNNNCTG 1 cut(s) 1613
Ama87I CYCGRG 1 cut(s) 1012
Aor13HI TCCGGA 2 cut(s) 718, 1515
AoxI GGCC 7 cut(s) 793, 1102, 1159, 1200, 1457, 1666, 1733
ApaI GGGCCC 1 cut(s) 1106
ApaLI GTGCAC 1 cut(s) 1431
ApeKI GCWGC 5 cut(s) 506, 1213, 1637, 1696, 1769
ApoI RAATTY 6 cut(s) 269, 321, 340, 714, 1319, 1703
AspLEI GCGC 2 cut(s) 55, 1027
AspS9I GGNCC 6 cut(s) 671, 1102, 1103, 1403, 1667, 1733
AsuHPI GGTGA 6 cut(s) 49, 597, 836, 1328, 1466, 1530
AvaI CYCGRG 1 cut(s) 1012
AvaII GGWCC 2 cut(s) 671, 1403
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 2 cut(s) 1106, 1435
BalI TGGCCA 1 cut(s) 1459
BamHI GGATCC 2 cut(s) 499, 1630
BanI GGYRCC 1 cut(s) 1024
BanII GRGCYC 1 cut(s) 1106
BarI GAAGNNNNNNTAC 2 cut(s) 1461, 1493
BbsI GAAGAC 3 cut(s) 637, 920, 1526
Bbv12I GWGCWC 1 cut(s) 1435
BbvCI CCTCAGC 1 cut(s) 1719
BbvI GCAGC 5 cut(s) 493, 1225, 1624, 1683, 1756
BccI CCATC 6 cut(s) 107, 401, 496, 550, 662, 785
BceAI ACGGC 2 cut(s) 1187, 1731
BciT130I CCWGG 2 cut(s) 933, 1749
BclI TGATCA 1 cut(s) 1615
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 3 cut(s) 174, 516, 1778
BfmI CTRYAG 2 cut(s) 381, 1368
BfoI RGCGCY 2 cut(s) 56, 1028
BfuAI ACCTGC 1 cut(s) 1120
BglII AGATCT 1 cut(s) 1110
BisI GCNGC 5 cut(s) 507, 1214, 1638, 1697, 1770
BlpI GCTNAGC 1 cut(s) 1773
BlsI GCNGC 5 cut(s) 508, 1215, 1639, 1698, 1771
Bme1390I CCNGG 2 cut(s) 933, 1749
Bme18I GGWCC 2 cut(s) 671, 1403
BmeT110I CYCGRG 1 cut(s) 1012
BmgT120I GGNCC 6 cut(s) 671, 1102, 1103, 1403, 1667, 1733
BmiI GGNNCC 6 cut(s) 501, 857, 1026, 1104, 1545, 1632
BmrFI CCNGG 2 cut(s) 933, 1749
BmsI GCATC 6 cut(s) 367, 455, 964, 1225, 1365, 1732
BpiI GAAGAC 3 cut(s) 637, 920, 1526
BpmI CTGGAG 4 cut(s) 381, 842, 1656, 1731
Bpu10I CCTNAGC 1 cut(s) 1719
Bpu1102I GCTNAGC 1 cut(s) 1773
BpuEI CTTGAG 2 cut(s) 1207, 1336
BsaHI GRCGYC 1 cut(s) 1025
BsaJI CCNNGG 2 cut(s) 1013, 1748
BsaWI WCCGGW 3 cut(s) 718, 1515, 1540
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 938, 1157
Bse118I RCCGGY 1 cut(s) 1281
Bse1I ACTGG 8 cut(s) 364, 371, 608, 928, 1140, 1394, 1432, 1673
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 1025
BseAI TCCGGA 2 cut(s) 718, 1515
BseBI CCWGG 2 cut(s) 933, 1749
BseDI CCNNGG 2 cut(s) 1013, 1748
BseGI GGATG 5 cut(s) 12, 118, 522, 561, 796
BseLI CCNNNNNNNGG 2 cut(s) 938, 1157
BseMI GCAATG 1 cut(s) 1025
BseMII CTCAG 8 cut(s) 12, 227, 276, 640, 960, 1301, 1591, 1710
BseNI ACTGG 8 cut(s) 364, 371, 608, 928, 1140, 1394, 1432, 1673
BseSI GKGCMC 2 cut(s) 1106, 1435
BseXI GCAGC 5 cut(s) 493, 1225, 1624, 1683, 1756
BseYI CCCAGC 1 cut(s) 1532
BsgI GTGCAG 2 cut(s) 1232, 1623
Bsh1285I CGRYCG 1 cut(s) 733
BshFI GGCC 7 cut(s) 795, 1104, 1161, 1202, 1459, 1668, 1735
BshNI GGYRCC 1 cut(s) 1024
BsiEI CGRYCG 1 cut(s) 733
BsiHKAI GWGCWC 1 cut(s) 1435
BsiHKCI CYCGRG 1 cut(s) 1012
BsiSI CCGG 4 cut(s) 719, 1282, 1516, 1541
BslFI GGGAC 3 cut(s) 173, 995, 1803
BslI CCNNNNNNNGG 2 cut(s) 938, 1157
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 3 cut(s) 173, 995, 1803
BsnI GGCC 7 cut(s) 795, 1104, 1161, 1202, 1459, 1668, 1735
BsoBI CYCGRG 1 cut(s) 1012
Bsp120I GGGCCC 1 cut(s) 1102
Bsp1286I GDGCHC 2 cut(s) 1106, 1435
Bsp13I TCCGGA 2 cut(s) 718, 1515
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
Bsp1720I GCTNAGC 1 cut(s) 1773
BspACI CCGC 3 cut(s) 247, 618, 822
BspANI GGCC 7 cut(s) 795, 1104, 1161, 1202, 1459, 1668, 1735
BspCNI CTCAG 8 cut(s) 13, 226, 277, 639, 959, 1302, 1590, 1711
BspEI TCCGGA 2 cut(s) 718, 1515
BspLI GGNNCC 6 cut(s) 501, 857, 1026, 1104, 1545, 1632
BspMI ACCTGC 1 cut(s) 1120
BspPI GGATC 6 cut(s) 246, 494, 507, 651, 1625, 1638
BspT107I GGYRCC 1 cut(s) 1024
BsrDI GCAATG 1 cut(s) 1025
BsrFI RCCGGY 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 8 cut(s) 364, 371, 608, 928, 1140, 1394, 1432, 1673
BssAI RCCGGY 1 cut(s) 1281
BssECI CCNNGG 2 cut(s) 1013, 1748
BssMI GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
BssNI GRCGYC 1 cut(s) 1025
Bst2UI CCWGG 2 cut(s) 933, 1749
Bst4CI ACNGT 5 cut(s) 426, 802, 865, 1468, 1609
Bst6I CTCTTC 2 cut(s) 6, 921
BstACI GRCGYC 1 cut(s) 1025
BstAUI TGTACA 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 1536
BstF5I GGATG 5 cut(s) 12, 118, 522, 561, 796
BstH2I RGCGCY 2 cut(s) 56, 1028
BstHHI GCGC 2 cut(s) 55, 1027
BstKTI GATC 8 cut(s) 241, 502, 625, 646, 733, 1113, 1618, 1633
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
BstMCI CGRYCG 1 cut(s) 733
BstMWI GCNNNNNNNGC 3 cut(s) 50, 792, 1024
BstNI CCWGG 2 cut(s) 933, 1749
BstNSI RCATGY 1 cut(s) 1811
BstPI GGTNACC 1 cut(s) 1536
BstSCI CCNGG 2 cut(s) 931, 1747
BstSFI CTRYAG 2 cut(s) 381, 1368
BstSLI GKGCMC 2 cut(s) 1106, 1435
BstV1I GCAGC 5 cut(s) 493, 1225, 1624, 1683, 1756
BstV2I GAAGAC 3 cut(s) 637, 920, 1526
BstX2I RGATCY 4 cut(s) 499, 643, 1110, 1630
BstXI CCANNNNNNTGG 3 cut(s) 495, 1284, 1755
BstYI RGATCY 4 cut(s) 499, 643, 1110, 1630
Bsu36I CCTNAGG 1 cut(s) 213
BsuRI GGCC 7 cut(s) 795, 1104, 1161, 1202, 1459, 1668, 1735
BtsCI GGATG 5 cut(s) 12, 118, 522, 561, 796
BtsI GCAGTG 3 cut(s) 85, 223, 943
BtsIMutI CAGTG 5 cut(s) 85, 223, 422, 943, 1680
BveI ACCTGC 1 cut(s) 1120
CaiI CAGNNNCTG 1 cut(s) 1613
CfoI GCGC 2 cut(s) 55, 1027
Cfr10I RCCGGY 1 cut(s) 1281
Cfr13I GGNCC 6 cut(s) 671, 1102, 1103, 1403, 1667, 1733
Csp6I GTAC 4 cut(s) 427, 473, 902, 1469
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 7 cut(s) 203, 754, 952, 1099, 1208, 1739, 1808
CviQI GTAC 4 cut(s) 427, 473, 902, 1469
DinI GGCGCC 1 cut(s) 1026
DpnI GATC 8 cut(s) 240, 501, 624, 645, 732, 1112, 1617, 1632
DpnII GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
EaeI YGGCCR 2 cut(s) 1200, 1457
Eam1104I CTCTTC 2 cut(s) 6, 921
EarI CTCTTC 2 cut(s) 6, 921
Eco24I GRGCYC 1 cut(s) 1106
Eco32I GATATC 1 cut(s) 335
Eco47I GGWCC 2 cut(s) 671, 1403
Eco57I CTGAAG 2 cut(s) 291, 312
Eco81I CCTNAGG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 1012
Eco91I GGTNACC 1 cut(s) 1536
EcoO109I RGGNCCY 2 cut(s) 1103, 1667
EcoO65I GGTNACC 1 cut(s) 1536
EcoRI GAATTC 3 cut(s) 269, 340, 1319
EcoRII CCWGG 2 cut(s) 931, 1747
EcoRV GATATC 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 759
EcoT38I GRGCYC 1 cut(s) 1106
EgeI GGCGCC 1 cut(s) 1026
EheI GGCGCC 1 cut(s) 1026
FaeI CATG 7 cut(s) 206, 757, 955, 1102, 1211, 1742, 1811
FalI AAGNNNNNCTT 4 cut(s) 1057, 1089, 1805, 1837
FaqI GGGAC 3 cut(s) 173, 995, 1803
FatI CATG 7 cut(s) 202, 753, 951, 1098, 1207, 1738, 1807
FauI CCCGC 1 cut(s) 625
FbaI TGATCA 1 cut(s) 1615
Fnu4HI GCNGC 5 cut(s) 507, 1214, 1638, 1697, 1770
FokI GGATG 5 cut(s) 19, 125, 509, 568, 803
FriOI GRGCYC 1 cut(s) 1106
Fsp4HI GCNGC 5 cut(s) 507, 1214, 1638, 1697, 1770
FspBI CTAG 3 cut(s) 174, 516, 1778
GlaI GCGC 2 cut(s) 54, 1026
GluI GCNGC 5 cut(s) 507, 1214, 1638, 1697, 1770
GsaI CCCAGC 1 cut(s) 1536
GsuI CTGGAG 4 cut(s) 381, 842, 1656, 1731
HaeII RGCGCY 2 cut(s) 56, 1028
HaeIII GGCC 7 cut(s) 795, 1104, 1161, 1202, 1459, 1668, 1735
HapII CCGG 4 cut(s) 719, 1282, 1516, 1541
HhaI GCGC 2 cut(s) 55, 1027
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 7 cut(s) 206, 757, 955, 1102, 1211, 1742, 1811
Hin6I GCGC 2 cut(s) 53, 1025
HinP1I GCGC 2 cut(s) 53, 1025
HinfI GANTC 1 cut(s) 243
HpaII CCGG 4 cut(s) 719, 1282, 1516, 1541
HphI GGTGA 6 cut(s) 49, 597, 836, 1328, 1466, 1530
Hpy166II GTNNAC 4 cut(s) 1433, 1590, 1596, 1711
Hpy8I GTNNAC 4 cut(s) 1433, 1590, 1596, 1711
HpyAV CCTTC 9 cut(s) 336, 684, 1089, 1172, 1238, 1390, 1487, 1557, 1658
HpyCH4III ACNGT 5 cut(s) 426, 802, 865, 1468, 1609
HpyCH4IV ACGT 5 cut(s) 779, 1562, 1586, 1592, 1792
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 792, 1024
HpySE526I ACGT 5 cut(s) 779, 1562, 1586, 1592, 1792
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 7 cut(s) 206, 757, 955, 1102, 1211, 1742, 1811
HspAI GCGC 2 cut(s) 53, 1025
KasI GGCGCC 1 cut(s) 1024
Kpn2I TCCGGA 2 cut(s) 718, 1515
Ksp22I TGATCA 1 cut(s) 1615
Kzo9I GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
LmnI GCTCC 3 cut(s) 861, 1543, 1750
Lsp1109I GCAGC 5 cut(s) 493, 1225, 1624, 1683, 1756
LweI GCATC 6 cut(s) 367, 455, 964, 1225, 1365, 1732
MaeI CTAG 3 cut(s) 174, 516, 1778
MaeII ACGT 5 cut(s) 779, 1562, 1586, 1592, 1792
MaeIII GTNAC 5 cut(s) 842, 1423, 1536, 1609, 1762
MalI GATC 8 cut(s) 240, 501, 624, 645, 732, 1112, 1617, 1632
MboI GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
MboII GAAGA 9 cut(s) 23, 297, 406, 591, 642, 908, 920, 1531, 1680
MfeI CAATTG 3 cut(s) 850, 1152, 1504
MflI RGATCY 4 cut(s) 499, 643, 1110, 1630
MhlI GDGCHC 2 cut(s) 1106, 1435
MlsI TGGCCA 1 cut(s) 1459
MluNI TGGCCA 1 cut(s) 1459
Mly113I GGCGCC 1 cut(s) 1025
MmeI TCCRAC 4 cut(s) 90, 544, 771, 1354
Mox20I TGGCCA 1 cut(s) 1459
Mph1103I ATGCAT 1 cut(s) 759
MroI TCCGGA 2 cut(s) 718, 1515
MscI TGGCCA 1 cut(s) 1459
MseI TTAA 5 cut(s) 294, 356, 894, 1335, 1644
MslI CAYNNNNRTG 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 1459
MspA1I CMGCKG 4 cut(s) 506, 620, 1637, 1769
MspI CCGG 4 cut(s) 719, 1282, 1516, 1541
MspR9I CCNGG 2 cut(s) 933, 1749
MunI CAATTG 3 cut(s) 850, 1152, 1504
MvaI CCWGG 2 cut(s) 933, 1749
MwoI GCNNNNNNNGC 3 cut(s) 50, 792, 1024
NarI GGCGCC 1 cut(s) 1025
NdeII GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
NlaIII CATG 7 cut(s) 206, 757, 955, 1102, 1211, 1742, 1811
NlaIV GGNNCC 6 cut(s) 501, 857, 1026, 1104, 1545, 1632
NmuCI GTSAC 2 cut(s) 842, 1536
NsiI ATGCAT 1 cut(s) 759
NspI RCATGY 1 cut(s) 1811
PfeI GAWTC 1 cut(s) 243
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 2 cut(s) 938, 1157
PkrI GCNGC 5 cut(s) 508, 1215, 1639, 1698, 1771
Ple19I CGATCG 1 cut(s) 733
PluTI GGCGCC 1 cut(s) 1028
PsiI TTATAA 1 cut(s) 966
Psp1406I AACGTT 1 cut(s) 1562
Psp6I CCWGG 2 cut(s) 931, 1747
PspEI GGTNACC 1 cut(s) 1536
PspFI CCCAGC 1 cut(s) 1532
PspGI CCWGG 2 cut(s) 931, 1747
PspN4I GGNNCC 6 cut(s) 501, 857, 1026, 1104, 1545, 1632
PspOMI GGGCCC 1 cut(s) 1102
PspPI GGNCC 6 cut(s) 671, 1102, 1103, 1403, 1667, 1733
PstNI CAGNNNCTG 1 cut(s) 1613
PsuI RGATCY 4 cut(s) 499, 643, 1110, 1630
PsyI GACNNNGTC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 733
PvuII CAGCTG 3 cut(s) 506, 1637, 1769
RsaI GTAC 4 cut(s) 428, 474, 903, 1470
RsaNI GTAC 4 cut(s) 427, 473, 902, 1469
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 5 cut(s) 294, 356, 894, 1335, 1644
SatI GCNGC 5 cut(s) 507, 1214, 1638, 1697, 1770
Sau3AI GATC 8 cut(s) 238, 499, 622, 643, 730, 1110, 1615, 1630
Sau96I GGNCC 6 cut(s) 671, 1102, 1103, 1403, 1667, 1733
ScrFI CCNGG 2 cut(s) 933, 1749
SduI GDGCHC 2 cut(s) 1106, 1435
SfaNI GCATC 6 cut(s) 367, 455, 964, 1225, 1365, 1732
SfcI CTRYAG 2 cut(s) 381, 1368
SfoI GGCGCC 1 cut(s) 1026
SinI GGWCC 2 cut(s) 671, 1403
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 1222, 1351
SmoI CTYRAG 2 cut(s) 1222, 1351
SsiI CCGC 3 cut(s) 247, 618, 822
SspDI GGCGCC 1 cut(s) 1024
SspMI CTAG 3 cut(s) 174, 516, 1778
StyD4I CCNGG 2 cut(s) 931, 1747
TaaI ACNGT 5 cut(s) 426, 802, 865, 1468, 1609
TaiI ACGT 5 cut(s) 782, 1565, 1589, 1595, 1795
TaqI TCGA 3 cut(s) 241, 729, 812
TatI WGTACW 2 cut(s) 426, 472
TfiI GAWTC 1 cut(s) 243
Tru1I TTAA 5 cut(s) 294, 356, 894, 1335, 1644
Tru9I TTAA 5 cut(s) 294, 356, 894, 1335, 1644
TscAI CASTG 5 cut(s) 85, 230, 429, 943, 1680
TseFI GTSAC 2 cut(s) 842, 1536
TseI GCWGC 5 cut(s) 506, 1213, 1637, 1696, 1769
Tsp45I GTSAC 2 cut(s) 842, 1536
TspDTI ATGAA 7 cut(s) 191, 406, 511, 519, 1087, 1196, 1353
TspGWI ACGGA 2 cut(s) 549, 1075
TspRI CASTG 5 cut(s) 85, 230, 429, 943, 1680
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 2 cut(s) 938, 1157
VneI GTGCAC 1 cut(s) 1431
VpaK11BI GGWCC 2 cut(s) 671, 1403
XapI RAATTY 6 cut(s) 269, 321, 340, 714, 1319, 1703
XceI RCATGY 1 cut(s) 1811
XspI CTAG 3 cut(s) 174, 516, 1778
Zsp2I ATGCAT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.