FvH4_4g19420
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017880
Physical Location & Seq
Reverse (-)
23069796 .. 23071313
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19420.t1

Sequence Viewer

Length: 582 bp
ATGTTTCTGGTGGATTGGTTCTACGGCGTGCTGGCATCTCTAGGACTGTGGCAGAAGGAAGCTAAGATCTTGTTCTTGGGGCTCGATAACGCCGGCAAGACCACCTTGCTTCACATGCTCAAAGATGAGAGACTAGTTCAGCACCAACCGACTCAGTACCCGACCTCCGAGGAGTTGAGTATAGGCCAGATTAAGTTCAAGGCTTTTGATTTGGGTGGCCATCAGATCGCTCGCCGAGTCTGGAAGGATTACTACGCCAAGGTAGATGCTGTTGTCTACCTGGTTGATGCTTTTGACAAGGAGAGATTTGCAGAGTCTAAGAAGGAGCTTGACGCTCTTCTTTCTGATGAGTCACTGGCCACTGTCCCGTTTCTTATTTTGGGGAACAAGATTGACATACCCTATGCCGCTTCGGAGGATGAGCTGCGTTATCATATGGGCCTGACCAACTTCACAACTGGTAAGGGGAAGGTCAATCTGGCAGATTCCAATGTTCGCCCACTGGAGGTGTTTATGTGCAGCATTGTCCGCAAGATGGGCTATGGCGAGGGATTCAAGTGGGTTTCTCAATACATCAAGTAG

Protein Analysis

194

Amino Acids

22.02

Weight (kDa)

6.43

Isoelectric Point (pI)

23.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 1.7e-63 ADP-ribosylation factor family
SRPRB PF09439 19 - 142 6e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 2.9e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 4.2e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 6.7e-07 Ras family
G-alpha PF00503 51 - 133 1.4e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62560
fragaria_vesca FvH4_4g19420
prunus_persica Prupe.1G122700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0424451
rosa_laevigata RLG00000007473
rosa_multiflora Rmu_sc0003567.1_g000008
rosa_roxburghii Rroxscaffold_5G00366390
rosa_samantha Rh4BG259300 Rh4CG270600 Rh4DG253800
rosa_wichuraiana Rw4G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AciI CCGC 2 cut(s) 408, 529
AcoI YGGCCR 2 cut(s) 217, 357
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 505, 535
AgsI TTSAA 2 cut(s) 199, 556
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 3 cut(s) 62, 328, 424
AluI AGCT 3 cut(s) 62, 328, 424
Alw26I GTCTC 1 cut(s) 124
AoxI GGCC 4 cut(s) 184, 217, 357, 439
ApeKI GCWGC 2 cut(s) 424, 519
AspS9I GGNCC 1 cut(s) 439
BalI TGGCCA 2 cut(s) 219, 359
BanII GRGCYC 1 cut(s) 84
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
BbvI GCAGC 2 cut(s) 411, 531
BccI CCATC 2 cut(s) 228, 529
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 124
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 2 cut(s) 41, 134
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 408, 425, 520
BlsI GCNGC 3 cut(s) 409, 426, 521
Bme1390I CCNGG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 3 cut(s) 44, 256, 277
BpmI CTGGAG 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 168, 258
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 535
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 360, 463, 507
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 168, 258
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 2 cut(s) 505, 535
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 3 cut(s) 360, 463, 507
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 411, 531
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 4 cut(s) 186, 219, 359, 441
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 505, 535
BsmAI GTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 4 cut(s) 186, 219, 359, 441
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 2 cut(s) 66, 225
BspACI CCGC 2 cut(s) 408, 529
BspANI GGCC 4 cut(s) 186, 219, 359, 441
BspCNI CTCAG 1 cut(s) 166
BspQI GCTCTTC 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 360, 463, 507
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 168, 258
BssMI GATC 2 cut(s) 66, 225
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 48, 364
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 4 cut(s) 29, 33, 94, 232
BstDEI CTNAG 3 cut(s) 63, 153, 318
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 2 cut(s) 69, 228
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 2 cut(s) 66, 225
BstMWI GCNNNNNNNGC 3 cut(s) 115, 528, 537
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 411, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 4 cut(s) 186, 219, 359, 441
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 3 cut(s) 353, 360, 500
Cac8I GCNNGC 4 cut(s) 29, 33, 94, 232
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 1 cut(s) 341
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 157
CviAII CATG 1 cut(s) 115
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 63, 153, 318
DpnI GATC 2 cut(s) 68, 227
DpnII GATC 2 cut(s) 66, 225
EaeI YGGCCR 2 cut(s) 217, 357
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 1 cut(s) 118
FaiI YATR 8 cut(s) 116, 182, 398, 405, 435, 437, 515, 543
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 114
FauNDI CATATG 1 cut(s) 435
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 3 cut(s) 408, 425, 520
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 3 cut(s) 408, 425, 520
FspBI CTAG 2 cut(s) 41, 134
GluI GCNGC 3 cut(s) 408, 425, 520
GsuI CTGGAG 1 cut(s) 524
HaeIII GGCC 4 cut(s) 186, 219, 359, 441
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 341
Hin1II CATG 1 cut(s) 118
HinfI GANTC 6 cut(s) 151, 237, 314, 350, 485, 552
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 169, 225, 346, 415
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 4 cut(s) 49, 238, 316, 463
HpyCH4III ACNGT 2 cut(s) 48, 364
HpyCH4V TGCA 2 cut(s) 311, 519
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 528, 537
HpyF3I CTNAG 3 cut(s) 63, 153, 318
Hsp92II CATG 1 cut(s) 118
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 2 cut(s) 66, 225
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 2 cut(s) 411, 531
LweI GCATC 3 cut(s) 44, 256, 277
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 2 cut(s) 41, 134
MaeIII GTNAC 1 cut(s) 351
MalI GATC 2 cut(s) 68, 227
MboI GATC 2 cut(s) 66, 225
MboII GAAGA 1 cut(s) 329
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 84
MlsI TGGCCA 2 cut(s) 219, 359
MluNI TGGCCA 2 cut(s) 219, 359
MlyI GAGTC 4 cut(s) 145, 246, 323, 359
MnlI CCTC 5 cut(s) 163, 175, 409, 499, 541
Mox20I TGGCCA 2 cut(s) 219, 359
MroNI GCCGGC 1 cut(s) 92
MscI TGGCCA 2 cut(s) 219, 359
MseI TTAA 1 cut(s) 192
Msp20I TGGCCA 2 cut(s) 219, 359
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 115, 528, 537
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 1 cut(s) 435
NdeII GATC 2 cut(s) 66, 225
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 1 cut(s) 351
NspI RCATGY 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 342
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 485, 552
PkrI GCNGC 3 cut(s) 409, 426, 521
PleI GAGTC 4 cut(s) 145, 245, 322, 358
PpsI GAGTC 4 cut(s) 145, 245, 322, 358
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 439
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 408, 425, 520
Sau3AI GATC 2 cut(s) 66, 225
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 4 cut(s) 145, 246, 323, 359
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 64, 107, 167, 264, 282, 330, 426, 474, 510
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 3 cut(s) 44, 256, 277
SpeI ACTAGT 1 cut(s) 133
SsiI CCGC 2 cut(s) 408, 529
SspMI CTAG 2 cut(s) 41, 134
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 48, 364
TaqI TCGA 1 cut(s) 84
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 485, 552
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 3 cut(s) 360, 367, 507
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 2 cut(s) 424, 519
Tsp45I GTSAC 1 cut(s) 351
TspRI CASTG 3 cut(s) 360, 367, 507
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 41, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.