Rw4G021930
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017880
Physical Location & Seq
Reverse (-)
46726606 .. 46728792
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021930.1

Sequence Viewer

Length: 582 bp
ATGTTTCTGGTGGATTGGTTCTACGGTGTTCTGGCATCCCTAGGGCTGTGGCAGAAGGAAGCTAAGATCTTGTTCTTGGGGCTCGATAATGCCGGGAAGACCACCTTGCTTCACATGCTCAAAGATGAGAGATTAGTTCAGCACCAACCGACTCAGTACCCGACCTCCGAGGAGTTGAGTATAGGGAAGATTAAGTTCAAGGCTTTTGATTTGGGTGGCCATCAGATCGCTCGCAGAGTCTGGAAGGATTATTACGCCAAGGTAGATGCTGTTGTCTACCTTGTTGATGCTTTTGACAAGGAGAGATTTGCAGAGTCAAAGAAGGAGCTCGACGCTCTTCTCTCCGATGAGTCACTGGCCACTGTCCCGTTTCTTATCTTGGGAAACAAGATTGACATACCCTATGCTGCCTCAGAGGATGAGCTGCGTTATCATATGGGCCTGACCAACTTCACAACTGGGAAGGGGAAGGTCAATCTGGTGGATTCCAATGTCCGTCCACTGGAGGTGTTTATGTGCAGTATTGTCCGCAAGATGGGCTATGGCGAAGGATTCAAGTGGGTTTCTCAATACATCAAGTAG

Protein Analysis

193

Amino Acids

22.05

Weight (kDa)

6.91

Isoelectric Point (pI)

22.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 1.4e-63 ADP-ribosylation factor family
SRPRB PF09439 19 - 142 4.2e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 2.9e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 4.1e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 8.7e-07 Ras family
G-alpha PF00503 51 - 133 1.6e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62560
fragaria_vesca FvH4_4g19420
prunus_persica Prupe.1G122700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0424451
rosa_laevigata RLG00000007473
rosa_multiflora Rmu_sc0003567.1_g000008
rosa_roxburghii Rroxscaffold_5G00366390
rosa_samantha Rh4BG259300 Rh4CG270600 Rh4DG253800
rosa_wichuraiana Rw4G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AciI CCGC 1 cut(s) 529
AcoI YGGCCR 2 cut(s) 217, 357
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 502, 535
AgsI TTSAA 2 cut(s) 199, 556
AluBI AGCT 3 cut(s) 62, 328, 424
AluI AGCT 3 cut(s) 62, 328, 424
Alw21I GWGCWC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 3 cut(s) 217, 357, 439
ApeKI GCWGC 2 cut(s) 407, 424
AspA2I CCTAGG 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 439
AsuC2I CCSGG 1 cut(s) 94
AvrII CCTAGG 1 cut(s) 40
BalI TGGCCA 2 cut(s) 219, 359
BanII GRGCYC 2 cut(s) 84, 330
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 394, 411
BccI CCATC 2 cut(s) 228, 529
BcnI CCSGG 1 cut(s) 94
BfaI CTAG 1 cut(s) 41
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 408, 425
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 2 cut(s) 409, 426
Bme1390I CCNGG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 3 cut(s) 44, 256, 277
BmuI ACTGGG 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 40, 168, 258
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 535
Bse1I ACTGG 3 cut(s) 360, 463, 507
BseDI CCNNGG 3 cut(s) 40, 168, 258
BseGI GGATG 2 cut(s) 35, 424
BseLI CCNNNNNNNGG 2 cut(s) 502, 535
BseMII CTCAG 2 cut(s) 167, 426
BseNI ACTGG 3 cut(s) 360, 463, 507
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 394, 411
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 3 cut(s) 219, 359, 441
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 502, 535
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 3 cut(s) 219, 359, 441
Bsp1286I GDGCHC 2 cut(s) 84, 330
Bsp143I GATC 2 cut(s) 66, 225
BspACI CCGC 1 cut(s) 529
BspANI GGCC 3 cut(s) 219, 359, 441
BspCNI CTCAG 2 cut(s) 166, 425
BspQI GCTCTTC 1 cut(s) 342
BsrI ACTGG 3 cut(s) 360, 463, 507
BssECI CCNNGG 3 cut(s) 40, 168, 258
BssMI GATC 2 cut(s) 66, 225
BssT1I CCWWGG 2 cut(s) 40, 258
Bst4CI ACNGT 2 cut(s) 26, 364
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 3 cut(s) 63, 153, 412
BstF5I GGATG 2 cut(s) 35, 424
BstKTI GATC 2 cut(s) 69, 228
BstMBI GATC 2 cut(s) 66, 225
BstMWI GCNNNNNNNGC 2 cut(s) 115, 537
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 394, 411
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 219, 359, 441
BtsCI GGATG 2 cut(s) 35, 424
BtsIMutI CAGTG 3 cut(s) 353, 360, 500
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 157
CviAII CATG 1 cut(s) 115
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 63, 153, 412
DpnI GATC 2 cut(s) 68, 227
DpnII GATC 2 cut(s) 66, 225
EaeI YGGCCR 2 cut(s) 217, 357
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 40, 258
Eco24I GRGCYC 2 cut(s) 84, 330
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 40, 258
EcoT38I GRGCYC 2 cut(s) 84, 330
ErhI CCWWGG 2 cut(s) 40, 258
FaeI CATG 1 cut(s) 118
FaiI YATR 8 cut(s) 116, 182, 398, 405, 435, 437, 515, 543
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 114
FauNDI CATATG 1 cut(s) 435
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 408, 425
FokI GGATG 2 cut(s) 22, 431
FriOI GRGCYC 2 cut(s) 84, 330
Fsp4HI GCNGC 2 cut(s) 408, 425
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 408, 425
GsuI CTGGAG 1 cut(s) 524
HaeIII GGCC 3 cut(s) 219, 359, 441
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 341
Hin1II CATG 1 cut(s) 118
HinfI GANTC 6 cut(s) 151, 237, 314, 350, 485, 552
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 2 cut(s) 277, 500
Hpy188I TCNGA 4 cut(s) 169, 225, 346, 415
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 2 cut(s) 277, 500
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 6 cut(s) 49, 238, 316, 457, 463, 542
HpyCH4III ACNGT 2 cut(s) 26, 364
HpyCH4V TGCA 2 cut(s) 311, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 537
HpyF3I CTNAG 3 cut(s) 63, 153, 412
Hsp92II CATG 1 cut(s) 118
Kzo9I GATC 2 cut(s) 66, 225
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 8 cut(s) 17, 106, 226, 341, 444, 455, 464, 488
Lsp1109I GCAGC 2 cut(s) 394, 411
LweI GCATC 3 cut(s) 44, 256, 277
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 351
MalI GATC 2 cut(s) 68, 227
MboI GATC 2 cut(s) 66, 225
MboII GAAGA 3 cut(s) 109, 199, 329
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 84, 330
MlsI TGGCCA 2 cut(s) 219, 359
MluNI TGGCCA 2 cut(s) 219, 359
MlyI GAGTC 4 cut(s) 145, 246, 323, 359
MnlI CCTC 5 cut(s) 163, 175, 409, 421, 499
Mox20I TGGCCA 2 cut(s) 219, 359
MscI TGGCCA 2 cut(s) 219, 359
MseI TTAA 1 cut(s) 192
Msp20I TGGCCA 2 cut(s) 219, 359
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 115, 537
NciI CCSGG 1 cut(s) 94
NdeI CATATG 1 cut(s) 435
NdeII GATC 2 cut(s) 66, 225
NlaIII CATG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 351
NspI RCATGY 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 485, 552
PkrI GCNGC 2 cut(s) 409, 426
PleI GAGTC 4 cut(s) 145, 245, 322, 358
PpsI GAGTC 4 cut(s) 145, 245, 322, 358
Psp124BI GAGCTC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 408, 425
Sau3AI GATC 2 cut(s) 66, 225
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 4 cut(s) 145, 246, 323, 359
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 2 cut(s) 84, 330
SetI ASST 9 cut(s) 64, 107, 167, 264, 282, 330, 426, 474, 510
SfaNI GCATC 3 cut(s) 44, 256, 277
SsiI CCGC 1 cut(s) 529
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 2 cut(s) 40, 258
TaaI ACNGT 2 cut(s) 26, 364
TaqI TCGA 2 cut(s) 84, 330
TfiI GAWTC 2 cut(s) 485, 552
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 3 cut(s) 360, 367, 507
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 2 cut(s) 407, 424
Tsp45I GTSAC 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 3 cut(s) 360, 367, 507
XceI RCATGY 1 cut(s) 118
XmaJI CCTAGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.