FvH4_4g19720

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016823
Physical Location & Seq
Reverse (-)
23265878 .. 23267284
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19720.t1

Sequence Viewer

Length: 1407 bp
ATGATCTCCAGAAAGATCTTATGTATTCTGATTCTGTTATCGTTGTCACTAAACACCATCCATGTTCTTGCCGATGACTCAGACGACAAGGACTCTAAGAAGGACTCAAACTCAGACAAAAAAGACTCGGGCTCGGACGAGCCTGAATCCTTTTTAGTATCTTGTGGCTCCGAAAATGAGGTAGAAGATTCCTCAGGCCGGAAATGGTCCCCCGATTCCAAGTTCCTAAACTCTTCCGACTTCTCATCGACGGAACAGCTCAACAAAACTGACCCGACTCTCCCCTCCGAAGATCCCTACAAGTCGGCACGAATCTTCAACGCCACATCCTCTTACATTTTCTCAGTCACGACCACAAAACGCCTCTGGATCAGACTCCACTTTTATCCATTCGAATACTCCAAGATCGATCCAGCCAAGGCCTATTTCTCCGTCGCCGCAAACGAGTTCACTCTCCTCAAAAACTACAGCGCTTTCACAAATGCCGCAGCTCTCACCATGGCCTACATTGTCAAAGAGTTCTCACTCACTCCTAGCACATGCGGGAACCTCAACATCACAATCACACCGTCTCCGCAACCGGAGGGCGCAATCGGTTTCCTCAACGGCATCGAGGTGATTCCGATGCCGGATGTTTTCAAGACAACTCCCTTGGTCGGCTTCCATGACCAGACTATCGAGGTCCAAAACTCCTCCGTACAAACCATGTACAGAGTCAACGTCGGCGGAGATTATGTTCCGCCAACGGAGGACGCTGGTGGGAACCGAACATGGTACGAAGACGGTGGCTACTTAATCGGTTCCCCCAACACTAAATACGGCGTCATCAGCGACGCCGATAGCAACAACGTCACCATTAAGTACGACGGAGATGAATACATGGCCCCAGAAGCTGTTTACAATACCGCGAGATCAATGGGGCCGAGTCCAAACGTGAACCTCAATACCAACCTGACGTGGGTTTTCGACGTCGATGCCAACTTCACGTACGTCGTGAGATTGCATTTCTGCGACTTTGAGCTGGAACTGACGAACCAGAGAGCGTTTGATATCTATCTCAACAACCACACGGCGCAGGAAGGCGCCGACGTGATAGCGTGGGCCGGCGGGGGCGGCTCCTCCGGGAAAGGGATCACTGTTTTTAAGGACTACGCGATTTATTTGAATGATCGCAATAATGGGAATGAGCAGATTTGGGTGGCGTTGCACCCTAATGAGAAATCAAAGCCTGAATACTATGATGCGTTGCTTAATGGATTAGAGGTCTTCAAGATCAACGACACATGTGGGAATTTTGGAAGCGAATATCCGGTGCCGGTAAGACATTACAGCTACAGCGAGGAGTTTCTGGAGAAGACAAAAGATGAATGCAAGCTGATAGGAGATCAAGACACTGACGATAACTGA

Protein Analysis

469

Amino Acids

52.08

Weight (kDa)

4.54

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 53 - 425 6.2e-28 Malectin-like domain
Malectin PF11721 236 - 397 1.7e-09 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016823)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19720
malus_domestica MD13G1154600.v1.1 MD16G1154400.v1.1
prunus_persica Prupe.1G117600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425211
rosa_laevigata RLG00000007385
rosa_multiflora Rmu_sc0004716.1_g000007
rosa_roxburghii Rroxscaffold_5G00367250
rosa_rugosa Rorug04G0201900
rosa_samantha Rh4AG258500 Rh4BG264300 Rh4CG276000 Rh4DG260400
rosa_wichuraiana Rw4G022400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 972
AccB1I GGYRCC 2 cut(s) 1082, 1312
AccII CGCG 2 cut(s) 908, 1154
AciI CCGC 9 cut(s) 438, 486, 543, 575, 726, 740, 906, 1107, 1113
AclWI GGATC 4 cut(s) 287, 377, 404, 1139
AcsI RAATTY 1 cut(s) 1291
AcyI GRCGYC 4 cut(s) 822, 834, 969, 1083
AfaI GTAC 5 cut(s) 699, 710, 776, 863, 989
AfeI AGCGCT 1 cut(s) 472
AfiI CCNNNNNNNGG 4 cut(s) 198, 656, 958, 1128
AflIII ACRYGT 1 cut(s) 1283
AgsI TTSAA 4 cut(s) 319, 640, 1165, 1270
AjiI CACGTC 2 cut(s) 957, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AloI GAACNNNNNNTCC 2 cut(s) 720, 752
AluBI AGCT 6 cut(s) 259, 491, 893, 1021, 1332, 1375
AluI AGCT 6 cut(s) 259, 491, 893, 1021, 1332, 1375
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 4 cut(s) 287, 377, 404, 1139
AlwNI CAGNNNCTG 1 cut(s) 893
Ama87I CYCGRG 1 cut(s) 127
Aor51HI AGCGCT 1 cut(s) 472
AoxI GGCC 6 cut(s) 196, 420, 501, 882, 920, 1101
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 1291
AspLEI GCGC 4 cut(s) 473, 590, 1075, 1085
AspS9I GGNCC 5 cut(s) 207, 682, 883, 920, 1101
AsuC2I CCSGG 1 cut(s) 1123
AsuHPI GGTGA 3 cut(s) 487, 628, 844
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 207, 682
AxyI CCTNAGG 1 cut(s) 193
BanI GGYRCC 2 cut(s) 1082, 1312
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 3 cut(s) 786, 1258, 1361
BbvI GCAGC 1 cut(s) 500
BccI CCATC 1 cut(s) 65
BceAI ACGGC 3 cut(s) 622, 835, 1086
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BcnI CCSGG 1 cut(s) 1123
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 466, 1333
BfoI RGCGCY 2 cut(s) 474, 1086
BglII AGATCT 1 cut(s) 15
BisI GCNGC 4 cut(s) 438, 486, 489, 1114
BlsI GCNGC 4 cut(s) 439, 487, 490, 1115
Bme1390I CCNGG 1 cut(s) 1123
Bme18I GGWCC 2 cut(s) 207, 682
BmeT110I CYCGRG 1 cut(s) 127
BmgBI CACGTC 2 cut(s) 957, 1090
BmgT120I GGNCC 5 cut(s) 207, 682, 883, 920, 1101
BmrFI CCNGG 1 cut(s) 1123
BmsI GCATC 4 cut(s) 615, 618, 964, 1231
BpiI GAAGAC 3 cut(s) 786, 1258, 1361
BpmI CTGGAG 1 cut(s) 1370
Bpu14I TTCGAA 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 1123
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 987
BsaBI GATNNNNATC 1 cut(s) 1053
BsaHI GRCGYC 4 cut(s) 822, 834, 969, 1083
BsaJI CCNNGG 3 cut(s) 417, 498, 651
BsaWI WCCGGW 2 cut(s) 580, 1309
BsaXI ACNNNNNCTCC 4 cut(s) 556, 586, 720, 750
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 656, 958, 1128
Bse118I RCCGGY 2 cut(s) 1103, 1315
Bse21I CCTNAGG 1 cut(s) 193
Bse8I GATNNNNATC 1 cut(s) 1053
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 3 cut(s) 417, 498, 651
BseGI GGATG 3 cut(s) 57, 326, 637
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 4 cut(s) 198, 656, 958, 1128
BseMII CTCAG 4 cut(s) 93, 126, 207, 357
BseRI GAGGAG 4 cut(s) 446, 682, 1108, 1355
BseXI GCAGC 1 cut(s) 500
Bsh1236I CGCG 2 cut(s) 908, 1154
BshFI GGCC 6 cut(s) 198, 422, 503, 884, 922, 1103
BshNI GGYRCC 2 cut(s) 1082, 1312
BshVI ATCGAT 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 7 cut(s) 199, 581, 629, 1104, 1122, 1310, 1316
BsiWI CGTACG 1 cut(s) 987
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 198, 656, 958, 1128
BsmAI GTCTC 1 cut(s) 576
BsmBI CGTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 1373
BsnI GGCC 6 cut(s) 198, 422, 503, 884, 922, 1103
BsoBI CYCGRG 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 134
Bsp1407I TGTACA 1 cut(s) 708
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 9 cut(s) 438, 486, 543, 575, 726, 740, 906, 1107, 1113
BspANI GGCC 6 cut(s) 198, 422, 503, 884, 922, 1103
BspCNI CTCAG 4 cut(s) 92, 125, 206, 356
BspDI ATCGAT 1 cut(s) 408
BspFNI CGCG 2 cut(s) 908, 1154
BspPI GGATC 4 cut(s) 287, 377, 404, 1139
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 2 cut(s) 1082, 1312
BsrFI RCCGGY 2 cut(s) 1103, 1315
BsrGI TGTACA 1 cut(s) 708
BssAI RCCGGY 2 cut(s) 1103, 1315
BssECI CCNNGG 3 cut(s) 417, 498, 651
BssNI GRCGYC 4 cut(s) 822, 834, 969, 1083
BssT1I CCWWGG 3 cut(s) 417, 498, 651
Bst4CI ACNGT 3 cut(s) 570, 785, 1138
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 4 cut(s) 822, 834, 969, 1083
BstAUI TGTACA 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 987
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 1105, 1373
BstDEI CTNAG 5 cut(s) 79, 96, 112, 193, 343
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 3 cut(s) 57, 326, 637
BstFNI CGCG 2 cut(s) 908, 1154
BstH2I RGCGCY 2 cut(s) 474, 1086
BstHHI GCGC 4 cut(s) 473, 590, 1075, 1085
BstMAI GTCTC 1 cut(s) 576
BstMWI GCNNNNNNNGC 3 cut(s) 828, 890, 1113
BstNSI RCATGY 2 cut(s) 543, 1287
BstSCI CCNGG 1 cut(s) 1121
BstSFI CTRYAG 2 cut(s) 466, 1333
BstUI CGCG 2 cut(s) 908, 1154
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 3 cut(s) 786, 1258, 1361
BstX2I RGATCY 2 cut(s) 15, 292
BstYI RGATCY 2 cut(s) 15, 292
Bsu15I ATCGAT 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 193
BsuRI GGCC 6 cut(s) 198, 422, 503, 884, 922, 1103
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 498
BtrI CACGTC 2 cut(s) 957, 1090
BtsCI GGATG 3 cut(s) 57, 326, 637
BtsIMutI CAGTG 2 cut(s) 1134, 1392
Cac8I GCNNGC 2 cut(s) 1105, 1373
CaiI CAGNNNCTG 1 cut(s) 893
CfoI GCGC 4 cut(s) 473, 590, 1075, 1085
Cfr10I RCCGGY 2 cut(s) 1103, 1315
Cfr13I GGNCC 5 cut(s) 207, 682, 883, 920, 1101
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 3 cut(s) 761, 811, 842
Csp6I GTAC 5 cut(s) 698, 709, 775, 862, 988
CviAII CATG 8 cut(s) 62, 499, 540, 665, 706, 771, 880, 1284
CviQI GTAC 5 cut(s) 698, 709, 775, 862, 988
DdeI CTNAG 5 cut(s) 79, 96, 112, 193, 343
DinI GGCGCC 1 cut(s) 1084
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 2 cut(s) 729, 741
Eco130I CCWWGG 3 cut(s) 417, 498, 651
Eco147I AGGCCT 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 134
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 2 cut(s) 207, 682
Eco47III AGCGCT 1 cut(s) 472
Eco81I CCTNAGG 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 127
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 3 cut(s) 417, 498, 651
EcoT38I GRGCYC 1 cut(s) 134
EgeI GGCGCC 1 cut(s) 1084
EheI GGCGCC 1 cut(s) 1084
ErhI CCWWGG 3 cut(s) 417, 498, 651
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 8 cut(s) 65, 502, 543, 668, 709, 774, 883, 1287
FaqI GGGAC 1 cut(s) 193
FatI CATG 8 cut(s) 61, 498, 539, 664, 705, 770, 879, 1283
FauI CCCGC 2 cut(s) 536, 1100
Fnu4HI GCNGC 4 cut(s) 438, 486, 489, 1114
FokI GGATG 3 cut(s) 44, 313, 644
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 4 cut(s) 438, 486, 489, 1114
FspBI CTAG 1 cut(s) 534
GlaI GCGC 4 cut(s) 472, 589, 1074, 1084
GluI GCNGC 4 cut(s) 438, 486, 489, 1114
GsuI CTGGAG 1 cut(s) 1370
HaeII RGCGCY 2 cut(s) 474, 1086
HaeIII GGCC 6 cut(s) 198, 422, 503, 884, 922, 1103
HapII CCGG 7 cut(s) 199, 581, 629, 1104, 1122, 1310, 1316
HgaI GACGC 3 cut(s) 761, 811, 842
HhaI GCGC 4 cut(s) 473, 590, 1075, 1085
Hin1I GRCGYC 4 cut(s) 822, 834, 969, 1083
Hin1II CATG 8 cut(s) 65, 502, 543, 668, 709, 774, 883, 1287
Hin6I GCGC 4 cut(s) 471, 588, 1073, 1083
HinP1I GCGC 4 cut(s) 471, 588, 1073, 1083
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HpaII CCGG 7 cut(s) 199, 581, 629, 1104, 1122, 1310, 1316
HphI GGTGA 3 cut(s) 487, 628, 844
Hpy166II GTNNAC 4 cut(s) 450, 718, 898, 937
Hpy188I TCNGA 9 cut(s) 30, 82, 115, 136, 172, 238, 289, 374, 624
Hpy188III TCNNGA 8 cut(s) 9, 349, 367, 640, 994, 1270, 1349, 1388
Hpy8I GTNNAC 4 cut(s) 450, 718, 898, 937
Hpy99I CGWCG 9 cut(s) 253, 437, 725, 836, 869, 971, 974, 995, 1091
HpyAV CCTTC 2 cut(s) 94, 1073
HpyCH4III ACNGT 3 cut(s) 570, 785, 1138
HpyCH4IV ACGT 8 cut(s) 720, 849, 933, 956, 969, 986, 990, 1089
HpyCH4V TGCA 3 cut(s) 1003, 1207, 1371
HpyF10VI GCNNNNNNNGC 3 cut(s) 828, 890, 1113
HpyF3I CTNAG 5 cut(s) 79, 96, 112, 193, 343
HpySE526I ACGT 8 cut(s) 720, 849, 933, 956, 969, 986, 990, 1089
Hsp92I GRCGYC 4 cut(s) 822, 834, 969, 1083
Hsp92II CATG 8 cut(s) 65, 502, 543, 668, 709, 774, 883, 1287
HspAI GCGC 4 cut(s) 471, 588, 1073, 1083
KasI GGCGCC 1 cut(s) 1082
KroI GCCGGC 1 cut(s) 1103
KroNI GCCGGC 1 cut(s) 1105
LmnI GCTCC 2 cut(s) 173, 1121
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 4 cut(s) 615, 618, 964, 1231
MaeI CTAG 1 cut(s) 534
MaeII ACGT 8 cut(s) 720, 849, 933, 956, 969, 986, 990, 1089
MaeIII GTNAC 3 cut(s) 45, 346, 850
MboII GAAGA 7 cut(s) 197, 225, 302, 307, 791, 1258, 1366
MflI RGATCY 2 cut(s) 15, 292
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 1 cut(s) 1291
Mly113I GGCGCC 1 cut(s) 1083
MlyI GAGTC 8 cut(s) 71, 86, 98, 119, 271, 369, 723, 934
MmeI TCCRAC 1 cut(s) 261
MroNI GCCGGC 1 cut(s) 1103
MseI TTAA 4 cut(s) 794, 858, 1143, 1251
MslI CAYNNNNRTG 2 cut(s) 614, 1212
MspI CCGG 7 cut(s) 199, 581, 629, 1104, 1122, 1310, 1316
MspR9I CCNGG 1 cut(s) 1123
Mva1269I GAATGC 1 cut(s) 1373
MvnI CGCG 2 cut(s) 908, 1154
MwoI GCNNNNNNNGC 3 cut(s) 828, 890, 1113
NaeI GCCGGC 1 cut(s) 1105
NarI GGCGCC 1 cut(s) 1083
NciI CCSGG 1 cut(s) 1123
NcoI CCATGG 1 cut(s) 498
NgoMIV GCCGGC 1 cut(s) 1103
NlaIII CATG 8 cut(s) 65, 502, 543, 668, 709, 774, 883, 1287
NmeAIII GCCGAG 1 cut(s) 948
NmuCI GTSAC 3 cut(s) 45, 346, 850
NspI RCATGY 2 cut(s) 543, 1287
NspV TTCGAA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 422
PciI ACATGT 1 cut(s) 1283
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PctI GAATGC 1 cut(s) 1373
PdiI GCCGGC 1 cut(s) 1105
PfeI GAWTC 6 cut(s) 31, 146, 188, 215, 312, 619
Pfl23II CGTACG 1 cut(s) 987
PfoI TCCNGGA 1 cut(s) 1121
PkrI GCNGC 4 cut(s) 439, 487, 490, 1115
PleI GAGTC 8 cut(s) 71, 86, 98, 119, 271, 369, 722, 933
PluTI GGCGCC 1 cut(s) 1086
PpsI GAGTC 8 cut(s) 71, 86, 98, 119, 271, 369, 722, 933
Ppu21I YACGTR 1 cut(s) 987
PscI ACATGT 1 cut(s) 1283
PspLI CGTACG 1 cut(s) 987
PspPI GGNCC 5 cut(s) 207, 682, 883, 920, 1101
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 2 cut(s) 15, 292
RsaI GTAC 5 cut(s) 699, 710, 776, 863, 989
RsaNI GTAC 5 cut(s) 698, 709, 775, 862, 988
RseI CAYNNNNRTG 2 cut(s) 614, 1212
SaqAI TTAA 4 cut(s) 794, 858, 1143, 1251
SatI GCNGC 4 cut(s) 438, 486, 489, 1114
Sau96I GGNCC 5 cut(s) 207, 682, 883, 920, 1101
SchI GAGTC 8 cut(s) 71, 86, 98, 119, 271, 369, 723, 934
ScrFI CCNGG 1 cut(s) 1123
SduI GDGCHC 1 cut(s) 134
SfaNI GCATC 4 cut(s) 615, 618, 964, 1231
SfcI CTRYAG 2 cut(s) 466, 1333
SfoI GGCGCC 1 cut(s) 1084
SfuI TTCGAA 1 cut(s) 393
SinI GGWCC 2 cut(s) 207, 682
SmiMI CAYNNNNRTG 2 cut(s) 614, 1212
Sse9I AATT 1 cut(s) 1291
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 9 cut(s) 438, 486, 543, 575, 726, 740, 906, 1107, 1113
SspDI GGCGCC 1 cut(s) 1082
SspMI CTAG 1 cut(s) 534
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 1 cut(s) 1121
StyI CCWWGG 3 cut(s) 417, 498, 651
TaaI ACNGT 3 cut(s) 570, 785, 1138
TaiI ACGT 8 cut(s) 723, 852, 936, 959, 972, 989, 993, 1092
TaqI TCGA 7 cut(s) 248, 393, 408, 612, 678, 966, 972
TasI AATT 1 cut(s) 1291
TatI WGTACW 1 cut(s) 708
TauI GCSGC 3 cut(s) 440, 488, 1116
TfiI GAWTC 6 cut(s) 31, 146, 188, 215, 312, 619
Tru1I TTAA 4 cut(s) 794, 858, 1143, 1251
Tru9I TTAA 4 cut(s) 794, 858, 1143, 1251
TscAI CASTG 2 cut(s) 1141, 1399
TseFI GTSAC 3 cut(s) 45, 346, 850
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 3 cut(s) 45, 346, 850
TspDTI ATGAA 2 cut(s) 888, 1380
TspGWI ACGGA 5 cut(s) 266, 421, 685, 761, 882
TspRI CASTG 2 cut(s) 1141, 1399
VpaK11BI GGWCC 2 cut(s) 207, 682
XapI RAATTY 1 cut(s) 1291
XceI RCATGY 2 cut(s) 543, 1287
XspI CTAG 1 cut(s) 534
ZraI GACGTC 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.