MD13G1154600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016823
Physical Location & Seq
Forward (+)
12057983 .. 12059317
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1154600.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGATCTACAAATCCCACATTTTGTTTCCTCTGGTTTTGTTATCCTTGTTCTCATCAAACACCGTTCGTGTTCGAAGCGAAGGCTCCGGCTCAGAGTCCTATATACTTTCCTGTGGCTCGTCTTCCGACGGGGAAACGGATTCTGATGGCCGGAAATGGACAACGGATTCCAAATACCTCGTCGCCTCCAACAATACGAAGACTGCGAAAGCCGAGTACCAAGACCCTGCCCTGCCATCCAAAGTCCCATTCACGACAGCACGAATCATGAACTCTGCAACATCATACAAGTTCTCTGTTCCATCAAGCAAACGCATGTTGGTCAGGTTCCAATTCTACCCATCCACGTACGACTCCGCCGATTCCTTGAACGGGGTTTTCGAAGTCACCGCCAACGGCCTCACCCTGCTTAAAAACTTCAGTCCTTTCATCACAGCGTTGGCCCTCACGCAGTACTACATCATAAGAGAGTACTCAATTGTCCCCGCCGAGTCCGGCATTCTCAATATCACGTTCACGCCGTCCCCGAATCGCGAAAATACGTTCGCTTTCGTGAACTCAGTGGAGGTGATCCCAATGGAGGAAATCTTCAAGCCGACCGACTTGATCGGCTTCCGAGGCCAAACTATCGACGTCCAAAACTCCTCCCTCCAGACAATGTACAGGTTAAGCGTCGGAGGGGAGTACATTCCCGCAAACAAGGATTCGGGACTCGCACGAACATGGTGGGACGACTCGCCATACCTACTAAATTCAGCTTACGGGGCCTCGTATTTCGACGGGGCATTTCTCGGGGTTTCGATCAAAGCTCCTGAAAATGTCACCATCGGACACACGAAGGACGTCCCCGAATATATTGCTCCTCTAACGGTGTACAAAACTGCGAGATCGATGGGGCCGAACCCTCACTTCAACGAGAAATACAACCTCACTTGGGTTTTTCAGGTGGACGCAAATTTCACCTACGTCGTTCGGTTCCATTTCTGCGACTTTCAAAACACACTGGTGAACCAGAGAACGTTCGATATATATCTCAACAATAAAACGGCGGAGGAGTCGGCGGATGTGATCCAGTGGGCCGGGGCAATCGGAATCCCGGTTTACAAGGATTATGCCACTCATGTGAACGACAGAGACGGTGATGATCTTCTTTGGGTGGGTTTGCATCCGAATGTGAAGACGCATCCGCAGTACTATGATGCCATACTCAATGGACTGGAGGTTTTTAAGGTTAATGACAAGCACGGGAACTTGGCGGGTTCAAATCCTGTGCCTTCAAAAATGCTTCTTAAGGCAGACGCTGCAGCCAGGGGTATACTGGCAAACACTGCCTAG

Protein Analysis

445

Amino Acids

49.37

Weight (kDa)

5.8

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 36 - 411 3.1e-30 Malectin-like domain
Malectin PF11721 281 - 377 1.7e-09 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016823)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19720
malus_domestica MD13G1154600.v1.1 MD16G1154400.v1.1
prunus_persica Prupe.1G117600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425211
rosa_laevigata RLG00000007385
rosa_multiflora Rmu_sc0004716.1_g000007
rosa_roxburghii Rroxscaffold_5G00367250
rosa_rugosa Rorug04G0201900
rosa_samantha Rh4AG258500 Rh4BG264300 Rh4CG276000 Rh4DG260400
rosa_wichuraiana Rw4G022400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 636, 846
AccI GTMKAC 1 cut(s) 1315
AccII CGCG 1 cut(s) 534
AciI CCGC 8 cut(s) 357, 390, 486, 693, 1049, 1061, 1187, 1256
AclI AACGTT 1 cut(s) 1019
AclWI GGATC 2 cut(s) 565, 1063
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 2 cut(s) 751, 955
AcuI CTGAAG 1 cut(s) 403
AcyI GRCGYC 2 cut(s) 633, 843
AfaI GTAC 8 cut(s) 218, 350, 455, 473, 662, 686, 875, 1193
AfiI CCNNNNNNNGG 3 cut(s) 372, 580, 934
AflII CTTAAG 1 cut(s) 1289
AgsI TTSAA 6 cut(s) 370, 592, 913, 995, 1263, 1278
AjnI CCWGG 1 cut(s) 1307
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AleI CACNNNNGTG 2 cut(s) 1004, 1121
AluBI AGCT 2 cut(s) 758, 809
AluI AGCT 2 cut(s) 758, 809
Alw26I GTCTC 1 cut(s) 1128
AlwI GGATC 2 cut(s) 565, 1063
AlwNI CAGNNNCTG 1 cut(s) 1301
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 7 cut(s) 148, 397, 441, 619, 765, 896, 1077
ApeKI GCWGC 2 cut(s) 1301, 1304
ApoI RAATTY 2 cut(s) 751, 955
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AspS9I GGNCC 4 cut(s) 442, 765, 896, 1077
AsuC2I CCSGG 2 cut(s) 1081, 1097
AsuHPI GGTGA 7 cut(s) 379, 394, 580, 814, 952, 1018, 1151
AsuII TTCGAA 2 cut(s) 73, 381
AvaI CYCGRG 1 cut(s) 791
BbsI GAAGAC 3 cut(s) 114, 206, 1184
BbvI GCAGC 2 cut(s) 1288, 1316
BccI CCATC 6 cut(s) 140, 244, 310, 349, 833, 886
BceAI ACGGC 3 cut(s) 412, 505, 1062
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 1 cut(s) 1309
BcnI CCSGG 2 cut(s) 1081, 1097
BcoDI GTCTC 1 cut(s) 1128
BfaI CTAG 1 cut(s) 1333
BfmI CTRYAG 1 cut(s) 1302
BfrI CTTAAG 1 cut(s) 1289
BisI GCNGC 2 cut(s) 1302, 1305
BlsI GCNGC 2 cut(s) 1303, 1306
BmcAI AGTACT 3 cut(s) 455, 473, 1193
Bme1390I CCNGG 3 cut(s) 1081, 1097, 1309
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 4 cut(s) 442, 765, 896, 1077
BmiI GGNNCC 5 cut(s) 85, 329, 766, 897, 977
BmrFI CCNGG 3 cut(s) 1081, 1097, 1309
BmsI GCATC 3 cut(s) 1174, 1189, 1192
BpiI GAAGAC 3 cut(s) 114, 206, 1184
BpmI CTGGAG 2 cut(s) 635, 1238
Bpu14I TTCGAA 2 cut(s) 73, 381
BpuMI CCSGG 2 cut(s) 1081, 1097
Bsa29I ATCGAT 1 cut(s) 890
BsaAI YACGTR 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 1029
BsaHI GRCGYC 2 cut(s) 633, 843
BsaJI CCNNGG 3 cut(s) 616, 1080, 1308
Bsc4I CCNNNNNNNGG 3 cut(s) 372, 580, 934
Bse1I ACTGG 4 cut(s) 1008, 1072, 1221, 1323
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 1 cut(s) 1309
BseCI ATCGAT 1 cut(s) 890
BseDI CCNNGG 3 cut(s) 616, 1080, 1308
BseGI GGATG 5 cut(s) 236, 341, 1069, 1165, 1183
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 3 cut(s) 372, 580, 934
BseMII CTCAG 2 cut(s) 105, 573
BseNI ACTGG 4 cut(s) 1008, 1072, 1221, 1323
BseRI GAGGAG 3 cut(s) 634, 852, 1067
BseXI GCAGC 2 cut(s) 1288, 1316
Bsh1236I CGCG 1 cut(s) 534
Bsh1285I CGRYCG 1 cut(s) 600
BshFI GGCC 7 cut(s) 150, 399, 443, 621, 767, 898, 1079
BshVI ATCGAT 1 cut(s) 890
BsiEI CGRYCG 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 5 cut(s) 87, 151, 495, 1080, 1097
BsiWI CGTACG 1 cut(s) 348
BslFI GGGAC 6 cut(s) 230, 467, 508, 723, 743, 830
BslI CCNNNNNNNGG 3 cut(s) 372, 580, 934
BsmAI GTCTC 1 cut(s) 1128
BsmBI CGTCTC 1 cut(s) 1128
BsmFI GGGAC 6 cut(s) 230, 467, 508, 723, 743, 830
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 7 cut(s) 150, 399, 443, 621, 767, 898, 1079
BsoBI CYCGRG 1 cut(s) 791
Bsp119I TTCGAA 2 cut(s) 73, 381
Bsp1407I TGTACA 2 cut(s) 660, 873
Bsp143I GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
Bsp68I TCGCGA 1 cut(s) 534
BspACI CCGC 8 cut(s) 357, 390, 486, 693, 1049, 1061, 1187, 1256
BspANI GGCC 7 cut(s) 150, 399, 443, 621, 767, 898, 1079
BspCNI CTCAG 2 cut(s) 104, 572
BspDI ATCGAT 1 cut(s) 890
BspFNI CGCG 1 cut(s) 534
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 5 cut(s) 85, 329, 766, 897, 977
BspMAI CTGCAG 1 cut(s) 1306
BspPI GGATC 2 cut(s) 565, 1063
BspT104I TTCGAA 2 cut(s) 73, 381
BspTI CTTAAG 1 cut(s) 1289
BsrGI TGTACA 2 cut(s) 660, 873
BsrI ACTGG 4 cut(s) 1008, 1072, 1221, 1323
BssECI CCNNGG 3 cut(s) 616, 1080, 1308
BssMI GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
BssNAI GTATAC 1 cut(s) 1316
BssNI GRCGYC 2 cut(s) 633, 843
Bst1107I GTATAC 1 cut(s) 1316
Bst2UI CCWGG 1 cut(s) 1309
Bst4CI ACNGT 3 cut(s) 64, 871, 1139
BstACI GRCGYC 2 cut(s) 633, 843
BstAFI CTTAAG 1 cut(s) 1289
BstAPI GCANNNNNTGC 2 cut(s) 1301, 1328
BstAUI TGTACA 2 cut(s) 660, 873
BstBAI YACGTR 1 cut(s) 348
BstBI TTCGAA 2 cut(s) 73, 381
BstDEI CTNAG 2 cut(s) 91, 559
BstF5I GGATG 5 cut(s) 236, 341, 1069, 1165, 1183
BstFNI CGCG 1 cut(s) 534
BstKTI GATC 7 cut(s) 6, 573, 609, 804, 890, 1071, 1147
BstMAI GTCTC 1 cut(s) 1128
BstMBI GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
BstMCI CGRYCG 1 cut(s) 600
BstMWI GCNNNNNNNGC 4 cut(s) 618, 764, 1301, 1328
BstNI CCWGG 1 cut(s) 1309
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 3 cut(s) 1079, 1095, 1307
BstSFI CTRYAG 1 cut(s) 1302
BstUI CGCG 1 cut(s) 534
BstV1I GCAGC 2 cut(s) 1288, 1316
BstV2I GAAGAC 3 cut(s) 114, 206, 1184
BstZ17I GTATAC 1 cut(s) 1316
Bsu15I ATCGAT 1 cut(s) 890
BsuRI GGCC 7 cut(s) 150, 399, 443, 621, 767, 898, 1079
BsuTUI ATCGAT 1 cut(s) 890
BtsCI GGATG 5 cut(s) 236, 341, 1069, 1165, 1183
BtsI GCAGTG 1 cut(s) 1326
BtsIMutI CAGTG 4 cut(s) 567, 1001, 1079, 1326
BtuMI TCGCGA 1 cut(s) 534
CaiI CAGNNNCTG 1 cut(s) 1301
CciI TCATGA 1 cut(s) 267
Cfr13I GGNCC 4 cut(s) 442, 765, 896, 1077
ClaI ATCGAT 1 cut(s) 890
CseI GACGC 4 cut(s) 661, 959, 1189, 1307
Csp6I GTAC 8 cut(s) 217, 349, 454, 472, 661, 685, 874, 1192
CviAII CATG 4 cut(s) 268, 316, 723, 1121
CviQI GTAC 8 cut(s) 217, 349, 454, 472, 661, 685, 874, 1192
DdeI CTNAG 2 cut(s) 91, 559
DpnI GATC 7 cut(s) 5, 572, 608, 803, 889, 1070, 1146
DpnII GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
EaeI YGGCCR 1 cut(s) 148
EciI GGCGGA 3 cut(s) 346, 1064, 1076
Eco57I CTGAAG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 1307
Esp3I CGTCTC 1 cut(s) 1128
FaeI CATG 4 cut(s) 271, 319, 726, 1124
FaqI GGGAC 6 cut(s) 230, 467, 508, 723, 743, 830
FatI CATG 4 cut(s) 267, 315, 722, 1120
FauI CCCGC 3 cut(s) 493, 700, 1249
FblI GTMKAC 1 cut(s) 1315
Fnu4HI GCNGC 2 cut(s) 1302, 1305
FokI GGATG 5 cut(s) 223, 328, 1076, 1152, 1170
Fsp4HI GCNGC 2 cut(s) 1302, 1305
FspBI CTAG 1 cut(s) 1333
GluI GCNGC 2 cut(s) 1302, 1305
GsuI CTGGAG 2 cut(s) 635, 1238
HaeIII GGCC 7 cut(s) 150, 399, 443, 621, 767, 898, 1079
HapII CCGG 5 cut(s) 87, 151, 495, 1080, 1097
HgaI GACGC 4 cut(s) 661, 959, 1189, 1307
Hin1I GRCGYC 2 cut(s) 633, 843
Hin1II CATG 4 cut(s) 271, 319, 726, 1124
HpaII CCGG 5 cut(s) 87, 151, 495, 1080, 1097
HphI GGTGA 7 cut(s) 379, 394, 580, 814, 952, 1018, 1151
Hpy166II GTNNAC 8 cut(s) 516, 556, 874, 949, 1009, 1102, 1126, 1316
Hpy188I TCNGA 8 cut(s) 94, 127, 145, 617, 677, 830, 1091, 1170
Hpy188III TCNNGA 7 cut(s) 253, 268, 533, 553, 652, 708, 812
Hpy8I GTNNAC 8 cut(s) 516, 556, 874, 949, 1009, 1102, 1126, 1316
Hpy99I CGWCG 6 cut(s) 131, 185, 635, 677, 782, 971
HpyAV CCTTC 3 cut(s) 74, 832, 1284
HpyCH4III ACNGT 3 cut(s) 64, 871, 1139
HpyCH4IV ACGT 7 cut(s) 347, 512, 542, 633, 843, 966, 1019
HpyCH4V TGCA 3 cut(s) 278, 1165, 1304
HpyF10VI GCNNNNNNNGC 4 cut(s) 618, 764, 1301, 1328
HpyF3I CTNAG 2 cut(s) 91, 559
HpySE526I ACGT 7 cut(s) 347, 512, 542, 633, 843, 966, 1019
Hsp92I GRCGYC 2 cut(s) 633, 843
Hsp92II CATG 4 cut(s) 271, 319, 726, 1124
Kzo9I GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
LmnI GCTCC 3 cut(s) 89, 814, 865
Lsp1109I GCAGC 2 cut(s) 1288, 1316
LweI GCATC 3 cut(s) 1174, 1189, 1192
MaeI CTAG 1 cut(s) 1333
MaeII ACGT 7 cut(s) 347, 512, 542, 633, 843, 966, 1019
MaeIII GTNAC 2 cut(s) 385, 820
MalI GATC 7 cut(s) 5, 572, 608, 803, 889, 1070, 1146
MboI GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
MboII GAAGA 5 cut(s) 114, 211, 580, 1139, 1189
MfeI CAATTG 1 cut(s) 477
MluCI AATT 4 cut(s) 332, 477, 751, 955
MlyI GAGTC 6 cut(s) 104, 347, 500, 705, 728, 1064
MmeI TCCRAC 3 cut(s) 150, 213, 655
MseI TTAA 5 cut(s) 411, 668, 1227, 1233, 1290
MslI CAYNNNNRTG 4 cut(s) 721, 1004, 1121, 1170
MspCI CTTAAG 1 cut(s) 1289
MspI CCGG 5 cut(s) 87, 151, 495, 1080, 1097
MspR9I CCNGG 3 cut(s) 1081, 1097, 1309
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 1 cut(s) 1309
MvnI CGCG 1 cut(s) 534
MwoI GCNNNNNNNGC 4 cut(s) 618, 764, 1301, 1328
NciI CCSGG 2 cut(s) 1081, 1097
NdeII GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
NlaIII CATG 4 cut(s) 271, 319, 726, 1124
NlaIV GGNNCC 5 cut(s) 85, 329, 766, 897, 977
NmeAIII GCCGAG 2 cut(s) 238, 514
NmuCI GTSAC 2 cut(s) 385, 820
NruI TCGCGA 1 cut(s) 534
NspI RCATGY 1 cut(s) 319
NspV TTCGAA 2 cut(s) 73, 381
OliI CACNNNNGTG 2 cut(s) 1004, 1121
PagI TCATGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 6 cut(s) 203, 357, 378, 518, 524, 896
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 7 cut(s) 140, 167, 264, 362, 529, 704, 1092
Pfl23II CGTACG 1 cut(s) 348
PkrI GCNGC 2 cut(s) 1303, 1306
PleI GAGTC 6 cut(s) 103, 347, 499, 705, 728, 1063
PpsI GAGTC 6 cut(s) 103, 347, 499, 705, 728, 1063
Ppu21I YACGTR 1 cut(s) 348
Psp1406I AACGTT 1 cut(s) 1019
Psp6I CCWGG 1 cut(s) 1307
PspGI CCWGG 1 cut(s) 1307
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 5 cut(s) 85, 329, 766, 897, 977
PspPI GGNCC 4 cut(s) 442, 765, 896, 1077
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 1 cut(s) 1301
RruI TCGCGA 1 cut(s) 534
RsaI GTAC 8 cut(s) 218, 350, 455, 473, 662, 686, 875, 1193
RsaNI GTAC 8 cut(s) 217, 349, 454, 472, 661, 685, 874, 1192
RseI CAYNNNNRTG 4 cut(s) 721, 1004, 1121, 1170
SaqAI TTAA 5 cut(s) 411, 668, 1227, 1233, 1290
SatI GCNGC 2 cut(s) 1302, 1305
Sau3AI GATC 7 cut(s) 3, 570, 606, 801, 887, 1068, 1144
Sau96I GGNCC 4 cut(s) 442, 765, 896, 1077
ScaI AGTACT 3 cut(s) 455, 473, 1193
SchI GAGTC 6 cut(s) 104, 347, 500, 705, 728, 1064
ScrFI CCNGG 3 cut(s) 1081, 1097, 1309
SfaNI GCATC 3 cut(s) 1174, 1189, 1192
SfcI CTRYAG 1 cut(s) 1302
SfuI TTCGAA 2 cut(s) 73, 381
SmiMI CAYNNNNRTG 4 cut(s) 721, 1004, 1121, 1170
SmlI CTYRAG 1 cut(s) 1289
SmoI CTYRAG 1 cut(s) 1289
Sse9I AATT 4 cut(s) 332, 477, 751, 955
SsiI CCGC 8 cut(s) 357, 390, 486, 693, 1049, 1061, 1187, 1256
SspMI CTAG 1 cut(s) 1333
StyD4I CCNGG 3 cut(s) 1079, 1095, 1307
TaaI ACNGT 3 cut(s) 64, 871, 1139
TaiI ACGT 7 cut(s) 350, 515, 545, 636, 846, 969, 1022
TaqI TCGA 7 cut(s) 73, 381, 630, 777, 800, 890, 1023
TaqII GACCGA 1 cut(s) 614
TasI AATT 4 cut(s) 332, 477, 751, 955
TatI WGTACW 6 cut(s) 453, 471, 660, 684, 873, 1191
TfiI GAWTC 7 cut(s) 140, 167, 264, 362, 529, 704, 1092
Tru1I TTAA 5 cut(s) 411, 668, 1227, 1233, 1290
Tru9I TTAA 5 cut(s) 411, 668, 1227, 1233, 1290
TscAI CASTG 4 cut(s) 567, 1008, 1079, 1333
TseFI GTSAC 2 cut(s) 385, 820
TseI GCWGC 2 cut(s) 1301, 1304
Tsp45I GTSAC 2 cut(s) 385, 820
TspDTI ATGAA 2 cut(s) 284, 418
TspGWI ACGGA 2 cut(s) 152, 179
TspRI CASTG 4 cut(s) 567, 1008, 1079, 1333
Vha464I CTTAAG 1 cut(s) 1289
XapI RAATTY 2 cut(s) 751, 955
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 1315
XmiI GTMKAC 1 cut(s) 1315
XspI CTAG 1 cut(s) 1333
ZraI GACGTC 2 cut(s) 634, 844
ZrmI AGTACT 3 cut(s) 455, 473, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.