FvH4_5g03420

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016327
Physical Location & Seq
Forward (+)
2045526 .. 2046888
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03420.t1

Sequence Viewer

Length: 651 bp
ATGGCCGAGAAAAACCAGCAGGCCTACGCATCATCAGCAAATGGCTACACCAGATCAAATGACCACGAATCGATCGGAGCCACCTTCCAATCCGAGGAGGAGCTGAAACGCCAGAAGAGAAGGAAGCTGTTTATGTATATTGGTATTTTCATTGTCTTCCAAATCATAGTTATGACCGTATTTGGTCTCACCGTCATGAAAGTAAAGACTCCCAAGCTCCGGTTAGGCAACATCGACGTCCAAACCCTCAACTATACCTCCCAAACGCCACCATCTTTCGATATGAGCTTCACAACCGAAATTAGAGTCAAGAACAAGAATTTCGGTCCCTACAAATATGATAGTGGCTATGTCACGTTTTTGTACCAGGGGATGCCATTGGTGCAGGTTACAATTCCTAAGGGAAAGGCTGGGTGGCGTTCGACTGAAAAAATTGGAGCCACCGCGATTATAAATTCCAAGGAGTTGCCGAGTGTTTCAAATCTTGGTAGTGATTTGGATTCAGGGGTGTTGAAGCTGAGCAGCCAAGCAAAGATTAGTGGAAAAGTTGAGTTGATGTTTGTGATGAAGAAGAAGAAAACTGCAGAGATGAATTGTAGTATTGAAGTTAATTTGGCAACCAAGGGGGTTCAGGCATTGGAATGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

217

Amino Acids

24.09

Weight (kDa)

9.64

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 376
AccII CGCG 1 cut(s) 446
AciI CCGC 1 cut(s) 444
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 319, 454
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 94, 219
AgsI TTSAA 3 cut(s) 480, 514, 605
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 5 cut(s) 103, 127, 217, 288, 517
AluI AGCT 5 cut(s) 103, 127, 217, 288, 517
Alw26I GTCTC 1 cut(s) 191
AoxI GGCC 2 cut(s) 3, 21
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 2 cut(s) 319, 454
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 534
BccI CCATC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 376
BisI GCNGC 1 cut(s) 523
BlpI GCTNAGC 1 cut(s) 518
BlsI GCNGC 1 cut(s) 524
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 79, 328, 439
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 2 cut(s) 38, 363
BpiI GAAGAC 1 cut(s) 148
Bpu1102I GCTNAGC 1 cut(s) 518
Bsa29I ATCGAT 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 93, 367, 459, 621
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 219
Bse21I CCTNAGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 368
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 4 cut(s) 93, 367, 459, 621
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 94, 219
BseMII CTCAG 1 cut(s) 509
BseRI GAGGAG 2 cut(s) 110, 113
BseXI GCAGC 1 cut(s) 534
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 404
Bsh1236I CGCG 1 cut(s) 446
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 5, 23
BshVI ATCGAT 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 220
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 2 cut(s) 94, 219
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 312
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 2 cut(s) 5, 23
Bso31I GGTCTC 1 cut(s) 191
Bsp143I GATC 2 cut(s) 53, 72
Bsp1720I GCTNAGC 1 cut(s) 518
BspACI CCGC 1 cut(s) 444
BspANI GGCC 2 cut(s) 5, 23
BspCNI CTCAG 1 cut(s) 510
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 1 cut(s) 446
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 3 cut(s) 79, 328, 439
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 93, 367, 459, 621
BssMI GATC 2 cut(s) 53, 72
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 459, 621
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 178, 193
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 399, 518
BstF5I GGATG 1 cut(s) 378
BstFNI CGCG 1 cut(s) 446
BstKTI GATC 2 cut(s) 56, 75
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 2 cut(s) 53, 72
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 2 cut(s) 35, 382
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 71
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 2 cut(s) 5, 23
BsuTUI ATCGAT 1 cut(s) 71
BtsCI GGATG 1 cut(s) 378
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 21
CciI TCATGA 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 326
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 1 cut(s) 364
CviAII CATG 1 cut(s) 196
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 399, 518
DpnI GATC 2 cut(s) 55, 74
DpnII GATC 2 cut(s) 53, 72
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 459, 621
Eco147I AGGCCT 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 326
Eco81I CCTNAGG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 2 cut(s) 459, 621
ErhI CCWWGG 2 cut(s) 459, 621
FaeI CATG 1 cut(s) 199
FaqI GGGAC 1 cut(s) 312
FatI CATG 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 523
GluI GCNGC 1 cut(s) 523
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 2 cut(s) 5, 23
HapII CCGG 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 1 cut(s) 199
HinfI GANTC 4 cut(s) 68, 208, 306, 500
HpaII CCGG 1 cut(s) 220
HphI GGTGA 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 77, 94
Hpy188III TCNNGA 2 cut(s) 196, 310
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 94, 114
HpyCH4III ACNGT 2 cut(s) 178, 193
HpyCH4IV ACGT 2 cut(s) 237, 356
HpyCH4V TGCA 3 cut(s) 385, 584, 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 382
HpyF3I CTNAG 2 cut(s) 399, 518
HpySE526I ACGT 2 cut(s) 237, 356
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 1 cut(s) 199
Kzo9I GATC 2 cut(s) 53, 72
LmnI GCTCC 4 cut(s) 77, 100, 222, 437
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 2 cut(s) 38, 363
MaeII ACGT 2 cut(s) 237, 356
MaeIII GTNAC 2 cut(s) 352, 388
MalI GATC 2 cut(s) 55, 74
MboI GATC 2 cut(s) 53, 72
MboII GAAGA 5 cut(s) 127, 148, 580, 583, 586
MluCI AATT 7 cut(s) 300, 319, 393, 432, 454, 592, 610
MlyI GAGTC 2 cut(s) 202, 315
MnlI CCTC 4 cut(s) 88, 91, 257, 268
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 3 cut(s) 170, 194, 640
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 368
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 2 cut(s) 35, 382
NdeII GATC 2 cut(s) 53, 72
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 3 cut(s) 79, 328, 439
NmeAIII GCCGAG 2 cut(s) 31, 495
NmuCI GTSAC 1 cut(s) 352
PagI TCATGA 1 cut(s) 195
PceI AGGCCT 1 cut(s) 23
PctI GAATGC 1 cut(s) 647
PfeI GAWTC 2 cut(s) 68, 500
PkrI GCNGC 1 cut(s) 524
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 2 cut(s) 202, 314
PpsI GAGTC 2 cut(s) 202, 314
PsiI TTATAA 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 3 cut(s) 79, 328, 439
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 586
PvuI CGATCG 1 cut(s) 75
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 3 cut(s) 170, 194, 640
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 2 cut(s) 53, 72
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 2 cut(s) 202, 315
ScrFI CCNGG 1 cut(s) 368
SfaNI GCATC 2 cut(s) 38, 363
SfcI CTRYAG 1 cut(s) 582
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 170, 194, 640
Sse9I AATT 7 cut(s) 300, 319, 393, 432, 454, 592, 610
SseBI AGGCCT 1 cut(s) 23
SsiI CCGC 1 cut(s) 444
StuI AGGCCT 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 2 cut(s) 459, 621
TaaI ACNGT 2 cut(s) 178, 193
TaiI ACGT 2 cut(s) 240, 359
TaqI TCGA 4 cut(s) 71, 234, 279, 422
TaqII GACCGA 1 cut(s) 314
TasI AATT 7 cut(s) 300, 319, 393, 432, 454, 592, 610
TfiI GAWTC 2 cut(s) 68, 500
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 139, 212, 581, 605
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 2 cut(s) 319, 454
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.