FvH4_5g14300

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018042
Physical Location & Seq
Reverse (-)
8088754 .. 8090787
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14300.t1

Sequence Viewer

Length: 2034 bp
ATGAGGCAGGGCGGCGGTTGGCTTCTTTGCACAACATTGCTGATACTACTTAGTCTCCTCTGTCTCTCCGTTGAGGCTAGACACCACAGCAACATCCCAAGAAGAGCCCTCAGGACTAGCAATATCCCCAGTACACAAGATTGGTACCATGCGGCAAAGATAGCATACCCTCCTAGCCAGGTTGTATTGGACAATGGCATTGTCCAACTCACATTTTCTAACCCTGGTGGTGAGATTACAGGAATCAAATATCACGTAATTGACAACTTACTCGGAAACAAGGCCAAACAGACAGCCTACTGGGATGTGATGTGGAGGGGCAAGCTTTACGGATTTCAGGGAACAGAATTTAGAGTAATTTCTGCGACTGAAGACCATGTTGAAATTTCTTTCATCAACACATACAATCCTTCCTTCTCTAATGACCAAATGATCCCTTTAACAGTTGACAAAAGGTACATAATACAGCGAGGTCGTTCAGGCTTCTATGCATACGCCATTTTCCAGCGAGATGAAGGGTGGCCGGAGCTGGCCATAAACGACGTTCGACTAGTTTTCAAGCTTCAGCAAGATCAATTCAACTACATGGCGGTATCGGATACTAGAAAAAGGTTCATGCCAACAGCTGACGACCGAAGCCTAAGTCAGCAACTGGCCTATCCTGAAGCTAAGCTTTTAAGCAATGCAACAAGCAATCCTCTCTTTTATGGAGAAGTGGATGACAAGTATCAGTACTCAACTGAAGACGAAGACGGCAAAGTCCATGGTTGGATTAGTACCAAGTCATCTGTGGGATTTTGGCTGATCACACCAAGTACTGAGTTTCGTGCTGGTGGACCTCTGAAGCAGGACCTCACCTCCCACGTCGGTCCGACGACAATGTGTGTGTTCTCTAGCAGTCATTACGCTTCAAAACAACTTGGATTGGTATTCCAACAAGGTGAAGCATGGAAAAAGGTATATGGACCTGTATTTGTGTATCTTAATCATGCTGGTCTAAATAGTGATCCCACTACTACACTTTGGGACAATGCTAAAGAGCAGATGCTTGAAGAGGTCAGAAGCTGGCCTTACAACTTCACTCAATGCCCCGATTTTCCTGCTTCGAACCAACGAGGATCGGTTTTCGGATACTTACAAGTAAACGATTGGTACATTAACAGAACCAGCCTCGTTCCGGCAAGCTCGGCTTATGTGGGATTGGCTGCACCTGGAGAAGTGGGATCATGGCAAACAGAAAGCAAGGGATACCAGTTTTGGACTCGAGCAGATGAAAATGGCTACTTTGTCATCAAGGATGTTAGACCTGGGAACTATAACTTGTATGCATGGGTTCCCGGGATTGTTGGAGATTACAAATATGAGGACGAGATTACTATTGAACCAGGAAGTCAAATCACGTTGCAGAAGCTTACTTACTGGCCTCCAAGAATTGGTCCTACATTATGGGAAATTGGCATCCCTGACCGGAGTTCTGCCGAGTTTTACATACCAGATCCATATCCAGCTCTGCTGAACAAACTCGATAACAATAATCACTCAGATGAGTCAGACAAGTTCAGGCAGTATGGGATTTGGGGACGTTATGGAGAATTATACCCTGAACATGATCTAGTCTACACTGTTGGTGTTAGCGATTACCGTTATGATTGGTTTTATGCTCAAGTGACTAGGGATACAGGAAAAGAAATGGTTGGCACCACATGGCAGATTCAATTTGAGCTTCCTAAAATGGCAGCTCCCGGAGATTATATGCTCCGACTGGCATTGGCCTCGGCTGAGTACGCAGAGCTGCAGATTCGAGTTAACTATGAAAACATGCCAGCTCTGTTCTCAACAGGGCGGATAGGAGAGGACAATGCTATAGCAAGGCATGGAATCCATGGTTTGTATTGGCTGTTCAATGTTAACGTACCAAGGTCTCAACTGCATGAAGGAAACAACACGCTGTATCTTACTCAGACAAGAGGTGAAAATACTTTCGTAGGAGTCATGTATGACTACATCAGACTAGAAGGACCTCCATTGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

678

Amino Acids

77.3

Weight (kDa)

5.55

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 57 - 239 2.5e-62 Rhamnogalacturonate lyase family
fn3_3 PF14686 395 - 468 5.9e-28 Polysaccharide lyase family 4, domain II
CBM-like PF14683 481 - 671 4.2e-49 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14300
rosa_chinensis RchiOBHm_Chr7g0182591
rosa_laevigata RLG00000005118
rosa_multiflora Rmu_co8464835.1_g000001 Rmu_sc0005811.1_g000008
rosa_rugosa Rorug06G0448400
rosa_samantha Rh7AG052200 Rh7BG051300 Rh7CG053300 Rh7DG051400
rosa_wichuraiana Rw7G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 758
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 2 cut(s) 144, 1697
AccB7I CCANNNNNTGG 1 cut(s) 1433
AccI GTMKAC 1 cut(s) 1617
AciI CCGC 5 cut(s) 12, 15, 152, 590, 1843
AclWI GGATC 5 cut(s) 427, 1001, 1126, 1231, 1490
AcoI YGGCCR 2 cut(s) 521, 531
AcsI RAATTY 2 cut(s) 347, 384
AcuI CTGAAG 5 cut(s) 390, 548, 684, 762, 863
AfaI GTAC 9 cut(s) 133, 146, 458, 734, 778, 817, 1154, 1784, 1914
AfiI CCNNNNNNNGG 2 cut(s) 1177, 1433
AgsI TTSAA 8 cut(s) 383, 559, 580, 912, 1052, 1382, 1715, 1903
AhlI ACTAGT 1 cut(s) 550
AjiI CACGTC 1 cut(s) 865
AjnI CCWGG 5 cut(s) 177, 223, 1210, 1306, 1384
Alw26I GTCTC 3 cut(s) 59, 68, 1926
AlwI GGATC 5 cut(s) 427, 1001, 1126, 1231, 1490
AlwNI CAGNNNCTG 2 cut(s) 652, 1065
Ama87I CYCGRG 2 cut(s) 1263, 1337
AoxI GGCC 7 cut(s) 282, 521, 531, 654, 1067, 1421, 1770
ApeKI GCWGC 3 cut(s) 1205, 1736, 1792
ApoI RAATTY 2 cut(s) 347, 384
ArsI GACNNNNNNTTYG 2 cut(s) 628, 660
Asp718I GGTACC 1 cut(s) 144
AspS9I GGNCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
AsuC2I CCSGG 3 cut(s) 1338, 1339, 1743
AsuHPI GGTGA 4 cut(s) 242, 847, 953, 1982
AsuII TTCGAA 1 cut(s) 1106
AvaI CYCGRG 2 cut(s) 1263, 1337
AvaII GGWCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
AxyI CCTNAGG 1 cut(s) 110
BalI TGGCCA 1 cut(s) 533
BanI GGYRCC 2 cut(s) 144, 1697
BanII GRGCYC 1 cut(s) 109
BarI GAAGNNNNNNTAC 2 cut(s) 1400, 1432
BbsI GAAGAC 3 cut(s) 378, 750, 756
BbvI GCAGC 3 cut(s) 1192, 1748, 1779
BceAI ACGGC 1 cut(s) 769
BcgI CGANNNNNNTGC 4 cut(s) 1082, 1116, 1755, 1789
BciT130I CCWGG 5 cut(s) 179, 225, 1212, 1308, 1386
BciVI GTATCC 4 cut(s) 592, 1124, 1241, 1669
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 3 cut(s) 1338, 1339, 1743
BcoDI GTCTC 3 cut(s) 59, 68, 1926
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 9 cut(s) 78, 117, 174, 551, 603, 894, 1613, 1671, 2012
BfmI CTRYAG 2 cut(s) 1793, 1863
BfuI GTATCC 4 cut(s) 592, 1124, 1241, 1669
BisI GCNGC 5 cut(s) 13, 153, 1206, 1737, 1793
BlpI GCTNAGC 1 cut(s) 669
BlsI GCNGC 5 cut(s) 14, 154, 1207, 1738, 1794
BmcAI AGTACT 2 cut(s) 734, 817
Bme1390I CCNGG 8 cut(s) 179, 225, 1212, 1308, 1338, 1339, 1386, 1743
Bme18I GGWCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
BmeT110I CYCGRG 2 cut(s) 1263, 1337
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
BmiI GGNNCC 3 cut(s) 146, 1335, 1699
BmrFI CCNGG 8 cut(s) 179, 225, 1212, 1308, 1338, 1339, 1386, 1743
BmrI ACTGGG 2 cut(s) 123, 310
BmsI GCATC 2 cut(s) 1035, 1467
BmuI ACTGGG 2 cut(s) 123, 310
BpiI GAAGAC 3 cut(s) 378, 750, 756
BpmI CTGGAG 1 cut(s) 1233
Bpu1102I GCTNAGC 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 1647
BpuMI CCSGG 3 cut(s) 1338, 1339, 1743
BsaAI YACGTR 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 1500
BsaI GGTCTC 1 cut(s) 1926
BsaJI CCNNGG 7 cut(s) 223, 763, 1307, 1337, 1773, 1882, 1916
BsaWI WCCGGW 1 cut(s) 1467
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 1177, 1433
Bse1I ACTGG 6 cut(s) 129, 305, 657, 1252, 1424, 1767
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 3 cut(s) 35, 688, 2024
Bse8I GATNNNNATC 1 cut(s) 1500
BseBI CCWGG 5 cut(s) 179, 225, 1212, 1308, 1386
BseDI CCNNGG 7 cut(s) 223, 763, 1307, 1337, 1773, 1882, 1916
BseGI GGATG 5 cut(s) 93, 310, 724, 1303, 1458
BseJI GATNNNNATC 1 cut(s) 1500
BseLI CCNNNNNNNGG 2 cut(s) 1177, 1433
BseMI GCAATG 3 cut(s) 35, 688, 2024
BseMII CTCAG 5 cut(s) 124, 810, 1554, 1770, 1973
BseNI ACTGG 6 cut(s) 129, 305, 657, 1252, 1424, 1767
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 1192, 1748, 1779
BsgI GTGCAG 1 cut(s) 1191
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 7 cut(s) 284, 523, 533, 656, 1069, 1423, 1772
BshNI GGYRCC 2 cut(s) 144, 1697
BsiEI CGRYCG 1 cut(s) 634
BsiHKCI CYCGRG 2 cut(s) 1263, 1337
BsiSI CCGG 5 cut(s) 524, 1178, 1338, 1468, 1743
BslFI GGGAC 2 cut(s) 1040, 1593
BslI CCNNNNNNNGG 2 cut(s) 1177, 1433
BsmAI GTCTC 3 cut(s) 59, 68, 1926
BsmFI GGGAC 2 cut(s) 1040, 1593
BsnI GGCC 7 cut(s) 284, 523, 533, 656, 1069, 1423, 1772
Bso31I GGTCTC 1 cut(s) 1926
BsoBI CYCGRG 2 cut(s) 1263, 1337
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
Bsp1720I GCTNAGC 1 cut(s) 669
Bsp19I CCATGG 2 cut(s) 763, 1882
BspACI CCGC 5 cut(s) 12, 15, 152, 590, 1843
BspANI GGCC 7 cut(s) 284, 523, 533, 656, 1069, 1423, 1772
BspCNI CTCAG 5 cut(s) 123, 811, 1553, 1771, 1972
BspLI GGNNCC 3 cut(s) 146, 1335, 1699
BspMAI CTGCAG 1 cut(s) 1797
BspPI GGATC 5 cut(s) 427, 1001, 1126, 1231, 1490
BspQI GCTCTTC 1 cut(s) 97
BspT104I TTCGAA 1 cut(s) 1106
BspT107I GGYRCC 2 cut(s) 144, 1697
BspTNI GGTCTC 1 cut(s) 1926
BsrDI GCAATG 3 cut(s) 35, 688, 2024
BsrI ACTGG 6 cut(s) 129, 305, 657, 1252, 1424, 1767
BssECI CCNNGG 7 cut(s) 223, 763, 1307, 1337, 1773, 1882, 1916
BssMI GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
BssT1I CCWWGG 3 cut(s) 763, 1882, 1916
Bst2UI CCWGG 5 cut(s) 179, 225, 1212, 1308, 1386
Bst4CI ACNGT 3 cut(s) 445, 1624, 1643
Bst6I CTCTTC 2 cut(s) 97, 1047
BstBAI YACGTR 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 5 cut(s) 323, 531, 1067, 1183, 1824
BstDEI CTNAG 8 cut(s) 50, 110, 641, 669, 819, 1540, 1779, 1959
BstDSI CCRYGG 2 cut(s) 763, 1882
BstF5I GGATG 5 cut(s) 93, 310, 724, 1303, 1458
BstKTI GATC 8 cut(s) 435, 574, 807, 1009, 1121, 1226, 1498, 1612
BstMAI GTCTC 3 cut(s) 59, 68, 1926
BstMBI GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 3 cut(s) 161, 1187, 1784
BstNI CCWGG 5 cut(s) 179, 225, 1212, 1308, 1386
BstNSI RCATGY 1 cut(s) 1822
BstSCI CCNGG 8 cut(s) 177, 223, 1210, 1306, 1336, 1337, 1384, 1741
BstSFI CTRYAG 2 cut(s) 1793, 1863
BstV1I GCAGC 3 cut(s) 1192, 1748, 1779
BstV2I GAAGAC 3 cut(s) 378, 750, 756
BstX2I RGATCY 1 cut(s) 1495
BstYI RGATCY 1 cut(s) 1495
Bsu36I CCTNAGG 1 cut(s) 110
BsuI GTATCC 4 cut(s) 592, 1124, 1241, 1669
BsuRI GGCC 7 cut(s) 284, 523, 533, 656, 1069, 1423, 1772
BtgI CCRYGG 2 cut(s) 763, 1882
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 5 cut(s) 93, 310, 724, 1303, 1458
BtsIMutI CAGTG 1 cut(s) 1620
Cac8I GCNNGC 5 cut(s) 323, 531, 1067, 1183, 1824
CaiI CAGNNNCTG 2 cut(s) 652, 1065
Cfr13I GGNCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
Cfr9I CCCGGG 1 cut(s) 1337
CpoI CGGWCCG 1 cut(s) 869
Csp6I GTAC 9 cut(s) 132, 145, 457, 733, 777, 816, 1153, 1783, 1913
CspI CGGWCCG 1 cut(s) 869
CviQI GTAC 9 cut(s) 132, 145, 457, 733, 777, 816, 1153, 1783, 1913
DdeI CTNAG 8 cut(s) 50, 110, 641, 669, 819, 1540, 1779, 1959
DpnI GATC 8 cut(s) 434, 573, 806, 1008, 1120, 1225, 1497, 1611
DpnII GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
DrdI GACNNNNNNGTC 1 cut(s) 758
DseDI GACNNNNNNGTC 1 cut(s) 758
EaeI YGGCCR 2 cut(s) 521, 531
Eam1104I CTCTTC 2 cut(s) 97, 1047
EarI CTCTTC 2 cut(s) 97, 1047
EciI GGCGGA 1 cut(s) 1858
Eco130I CCWWGG 3 cut(s) 763, 1882, 1916
Eco24I GRGCYC 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 1926
Eco47I GGWCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
Eco57I CTGAAG 5 cut(s) 390, 548, 684, 762, 863
Eco81I CCTNAGG 1 cut(s) 110
Eco88I CYCGRG 2 cut(s) 1263, 1337
EcoO109I RGGNCCY 2 cut(s) 850, 2018
EcoRII CCWGG 5 cut(s) 177, 223, 1210, 1306, 1384
EcoT14I CCWWGG 3 cut(s) 763, 1882, 1916
EcoT22I ATGCAT 2 cut(s) 493, 1330
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 3 cut(s) 763, 1882, 1916
FalI AAGNNNNNCTT 6 cut(s) 657, 689, 1054, 1086, 1174, 1206
FaqI GGGAC 2 cut(s) 1040, 1593
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 1617
Fnu4HI GCNGC 5 cut(s) 13, 153, 1206, 1737, 1793
FokI GGATG 5 cut(s) 80, 317, 731, 1310, 1445
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 5 cut(s) 13, 153, 1206, 1737, 1793
FspBI CTAG 9 cut(s) 78, 117, 174, 551, 603, 894, 1613, 1671, 2012
GluI GCNGC 5 cut(s) 13, 153, 1206, 1737, 1793
GsuI CTGGAG 1 cut(s) 1233
HaeIII GGCC 7 cut(s) 284, 523, 533, 656, 1069, 1423, 1772
HapII CCGG 5 cut(s) 524, 1178, 1338, 1468, 1743
HincII GTYRAC 3 cut(s) 448, 1807, 1909
HindII GTYRAC 3 cut(s) 448, 1807, 1909
HindIII AAGCTT 4 cut(s) 323, 560, 671, 1409
HinfI GANTC 7 cut(s) 243, 1261, 1547, 1711, 1798, 1878, 1989
HpaI GTTAAC 2 cut(s) 1807, 1909
HpaII CCGG 5 cut(s) 524, 1178, 1338, 1468, 1743
HphI GGTGA 4 cut(s) 242, 847, 953, 1982
Hpy166II GTNNAC 7 cut(s) 134, 448, 836, 1144, 1618, 1807, 1909
Hpy188III TCNNGA 2 cut(s) 112, 662
Hpy8I GTNNAC 7 cut(s) 134, 448, 836, 1144, 1618, 1807, 1909
Hpy99I CGWCG 3 cut(s) 545, 869, 877
HpyAV CCTTC 5 cut(s) 420, 424, 509, 1928, 2009
HpyCH4III ACNGT 3 cut(s) 445, 1624, 1643
HpyCH4IV ACGT 6 cut(s) 255, 543, 864, 1400, 1582, 1911
HpyCH4V TGCA 8 cut(s) 30, 491, 686, 1208, 1328, 1405, 1795, 1930
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 1187, 1784
HpyF3I CTNAG 8 cut(s) 50, 110, 641, 669, 819, 1540, 1779, 1959
HpySE526I ACGT 6 cut(s) 255, 543, 864, 1400, 1582, 1911
KpnI GGTACC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 804
KspAI GTTAAC 2 cut(s) 1807, 1909
Kzo9I GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 3 cut(s) 526, 1744, 1761
Lsp1109I GCAGC 3 cut(s) 1192, 1748, 1779
LweI GCATC 2 cut(s) 1035, 1467
MaeI CTAG 9 cut(s) 78, 117, 174, 551, 603, 894, 1613, 1671, 2012
MaeII ACGT 6 cut(s) 255, 543, 864, 1400, 1582, 1911
MaeIII GTNAC 1 cut(s) 1666
MalI GATC 8 cut(s) 434, 573, 806, 1008, 1120, 1225, 1497, 1611
MboI GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
MboII GAAGA 5 cut(s) 114, 383, 755, 761, 1064
MflI RGATCY 1 cut(s) 1495
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 9 cut(s) 258, 347, 357, 384, 575, 1431, 1452, 1592, 1715
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 3 cut(s) 1255, 1556, 1998
MmeI TCCRAC 6 cut(s) 229, 749, 896, 958, 1327, 1783
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 2 cut(s) 493, 1330
MscI TGGCCA 1 cut(s) 533
MseI TTAA 6 cut(s) 440, 677, 984, 1158, 1806, 1908
MslI CAYNNNNRTG 2 cut(s) 1542, 2029
Msp20I TGGCCA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 5 cut(s) 524, 1178, 1338, 1468, 1743
MspR9I CCNGG 8 cut(s) 179, 225, 1212, 1308, 1338, 1339, 1386, 1743
MvaI CCWGG 5 cut(s) 179, 225, 1212, 1308, 1386
MwoI GCNNNNNNNGC 3 cut(s) 161, 1187, 1784
NciI CCSGG 3 cut(s) 1338, 1339, 1743
NcoI CCATGG 2 cut(s) 763, 1882
NdeII GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
NlaIV GGNNCC 3 cut(s) 146, 1335, 1699
NmeAIII GCCGAG 3 cut(s) 1166, 1504, 1754
NmuCI GTSAC 1 cut(s) 1666
NsiI ATGCAT 2 cut(s) 493, 1330
NspI RCATGY 1 cut(s) 1822
NspV TTCGAA 1 cut(s) 1106
PaeR7I CTCGAG 1 cut(s) 1263
PciSI GCTCTTC 1 cut(s) 97
PfeI GAWTC 4 cut(s) 243, 1711, 1798, 1878
PflMI CCANNNNNTGG 1 cut(s) 1433
PfoI TCCNGGA 1 cut(s) 1741
PkrI GCNGC 5 cut(s) 14, 154, 1207, 1738, 1794
PleI GAGTC 3 cut(s) 1255, 1555, 1997
PpsI GAGTC 3 cut(s) 1255, 1555, 1997
Ppu21I YACGTR 1 cut(s) 256
PpuMI RGGWCCY 2 cut(s) 850, 2018
Psp5II RGGWCCY 2 cut(s) 850, 2018
Psp6I CCWGG 5 cut(s) 177, 223, 1210, 1306, 1384
PspGI CCWGG 5 cut(s) 177, 223, 1210, 1306, 1384
PspN4I GGNNCC 3 cut(s) 146, 1335, 1699
PspPI GGNCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
PspPPI RGGWCCY 2 cut(s) 850, 2018
PspXI VCTCGAGB 1 cut(s) 1263
PsrI GAACNNNNNNTAC 2 cut(s) 1883, 1915
PstI CTGCAG 1 cut(s) 1797
PstNI CAGNNNCTG 2 cut(s) 652, 1065
PsuI RGATCY 1 cut(s) 1495
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 9 cut(s) 133, 146, 458, 734, 778, 817, 1154, 1784, 1914
RsaNI GTAC 9 cut(s) 132, 145, 457, 733, 777, 816, 1153, 1783, 1913
RseI CAYNNNNRTG 2 cut(s) 1542, 2029
Rsr2I CGGWCCG 1 cut(s) 869
RsrII CGGWCCG 1 cut(s) 869
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 6 cut(s) 440, 677, 984, 1158, 1806, 1908
SatI GCNGC 5 cut(s) 13, 153, 1206, 1737, 1793
Sau3AI GATC 8 cut(s) 432, 571, 804, 1006, 1118, 1223, 1495, 1609
Sau96I GGNCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
ScaI AGTACT 2 cut(s) 734, 817
SchI GAGTC 3 cut(s) 1255, 1556, 1998
ScrFI CCNGG 8 cut(s) 179, 225, 1212, 1308, 1338, 1339, 1386, 1743
SduI GDGCHC 1 cut(s) 109
SfaNI GCATC 2 cut(s) 1035, 1467
SfcI CTRYAG 2 cut(s) 1793, 1863
Sfr274I CTCGAG 1 cut(s) 1263
SfuI TTCGAA 1 cut(s) 1106
SinI GGWCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
SlaI CTCGAG 1 cut(s) 1263
SmaI CCCGGG 1 cut(s) 1339
SmiMI CAYNNNNRTG 2 cut(s) 1542, 2029
SmlI CTYRAG 2 cut(s) 1263, 1662
SmoI CTYRAG 2 cut(s) 1263, 1662
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 9 cut(s) 258, 347, 357, 384, 575, 1431, 1452, 1592, 1715
SsiI CCGC 5 cut(s) 12, 15, 152, 590, 1843
SspMI CTAG 9 cut(s) 78, 117, 174, 551, 603, 894, 1613, 1671, 2012
StyD4I CCNGG 8 cut(s) 177, 223, 1210, 1306, 1336, 1337, 1384, 1741
StyI CCWWGG 3 cut(s) 763, 1882, 1916
TaaI ACNGT 3 cut(s) 445, 1624, 1643
TaiI ACGT 6 cut(s) 258, 546, 867, 1403, 1585, 1914
TaqI TCGA 5 cut(s) 547, 1106, 1264, 1524, 1801
TaqII GACCGA 2 cut(s) 648, 857
TasI AATT 9 cut(s) 258, 347, 357, 384, 575, 1431, 1452, 1592, 1715
TatI WGTACW 3 cut(s) 131, 732, 815
TauI GCSGC 2 cut(s) 15, 155
TfiI GAWTC 4 cut(s) 243, 1711, 1798, 1878
Tru1I TTAA 6 cut(s) 440, 677, 984, 1158, 1806, 1908
Tru9I TTAA 6 cut(s) 440, 677, 984, 1158, 1806, 1908
TscAI CASTG 1 cut(s) 1627
TseFI GTSAC 1 cut(s) 1666
TseI GCWGC 3 cut(s) 1205, 1736, 1792
Tsp45I GTSAC 1 cut(s) 1666
TspDTI ATGAA 6 cut(s) 382, 528, 604, 1287, 1827, 1947
TspGWI ACGGA 2 cut(s) 58, 345
TspMI CCCGGG 1 cut(s) 1337
TspRI CASTG 1 cut(s) 1627
Van91I CCANNNNNTGG 1 cut(s) 1433
VpaK11BI GGWCC 6 cut(s) 836, 850, 869, 965, 1436, 2018
XapI RAATTY 2 cut(s) 347, 384
XceI RCATGY 1 cut(s) 1822
XcmI CCANNNNNNNNNTGG 1 cut(s) 787
XhoI CTCGAG 1 cut(s) 1263
XmaI CCCGGG 1 cut(s) 1337
XmiI GTMKAC 1 cut(s) 1617
XspI CTAG 9 cut(s) 78, 117, 174, 551, 603, 894, 1613, 1671, 2012
ZrmI AGTACT 2 cut(s) 734, 817
Zsp2I ATGCAT 2 cut(s) 493, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.