Rmu_co8464835.1_g000001

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8464835.1
OG0018042
Physical Location & Seq
Reverse (-)
407 .. 1855
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8464835.1_g000001.1.cds

Sequence Viewer

Length: 1449 bp
atggcggtgtcggatactagaagaaggttcatgccaacagctgacgaccgaaacctaggtcagcaacttgcctatcctgaagctaagcttttaaacaatgcaacaagcaatcctctctttcatggcgaagtggatgacaagtaccagtactcaagtgatgacaaaaatggcaaagtccatggctggattagtaccaagtcatctgtgggattttggttgatcacaccaagtactgagtttcgtgctggtggacctccgaagcaggacctcacctcccatgtcgggccgacgacaatgtgtgtgttctctagtagtcattacgctacagaacaactttgtttgaaattcaaacaaggtgaagcatggaaaaaggtttatggacctgtgtatgtgtatcttaatctagctggttcaaccagggatcccactactacactttggcacgacgctaagcaacagatgcatgaagaggtgagaagctggccttacaattttactcgatgcccagattttcctgtttcggagcaacgaggatcagtttttggaaatttacaagtaaatgataggtacattaacagcaccagccttgttccggcaagctcggcttatgtgggattggctgcacctggagaagtgggatcatggcaaacagaaagcaagggataccagttttggactcgagccgatgcaaaaggcgactttgtcatcaaacatgttcgacctgggaactataatttgtatgcatgggttcccgggattcttggagattacaaatatgaggtcgagattagtattaaaccaggaagtcgattcgagttggataagcttacttattcgcctccaagaattggtcctacattatgggaaatcggcatccctgatcgaagtgctgccgagttttacataccggatccatatccaactctcatgaacaaacttgataacaataatcactcggatgcctcggacaagttcaggcaatatgggatctggggacgttatggagagttataccctgaccatgatctggtctacactgttggtgttagcgattatcgtcatgattggttttatgctcatgtgactaggaatacaggaaacgtcttggtcggaaccacatggcagattcaatttaagcttactaatatgacagctcccggagattataagctccgattggcattggcctccgccaactatgcagagttggaggttcgatttaacaaagaaagcaaggcacctcttttctctacagggcggataggagaggacaatgctatagcaagacatggaattcatggtttatattggttgttcgatgttaatgtaccaaggtctcaactgcatgaaggaaacaacacactctatcttactcagacaagaggcggaaatatgttcgtaggagtcatgtatgactacatcagactagaaggacctccattgcgatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

482

Amino Acids

54.71

Weight (kDa)

6.93

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14300
rosa_chinensis RchiOBHm_Chr7g0182591
rosa_laevigata RLG00000005118
rosa_multiflora Rmu_co8464835.1_g000001 Rmu_sc0005811.1_g000008
rosa_rugosa Rorug06G0448400
rosa_samantha Rh7AG052200 Rh7BG051300 Rh7CG053300 Rh7DG051400
rosa_wichuraiana Rw7G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
AccB1I GGYRCC 1 cut(s) 1237
AccB7I CCANNNNNTGG 2 cut(s) 848, 1027
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 4 cut(s) 5, 1191, 1258, 1386
AclWI GGATC 7 cut(s) 416, 429, 541, 646, 905, 918, 995
AcsI RAATTY 3 cut(s) 344, 547, 1293
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 6 cut(s) 143, 149, 193, 232, 569, 1329
AfiI CCNNNNNNNGG 4 cut(s) 282, 592, 848, 1027
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 343, 349, 414, 1130
AjnI CCWGG 4 cut(s) 416, 625, 721, 799
Alw26I GTCTC 1 cut(s) 1341
AlwI GGATC 7 cut(s) 416, 429, 541, 646, 905, 918, 995
Ama87I CYCGRG 2 cut(s) 678, 752
AoxI GGCC 3 cut(s) 284, 482, 1185
ApeKI GCWGC 2 cut(s) 620, 890
ApoI RAATTY 3 cut(s) 344, 547, 1293
ArsI GACNNNNNNTTYG 4 cut(s) 43, 75, 765, 797
AspA2I CCTAGG 1 cut(s) 55
AspS9I GGNCC 6 cut(s) 251, 265, 284, 380, 851, 1433
AsuC2I CCSGG 3 cut(s) 753, 754, 1158
AsuHPI GGTGA 3 cut(s) 262, 368, 484
AvaI CYCGRG 2 cut(s) 678, 752
AvaII GGWCC 5 cut(s) 251, 265, 380, 851, 1433
AvrII CCTAGG 1 cut(s) 55
BamHI GGATCC 2 cut(s) 421, 910
BanI GGYRCC 1 cut(s) 1237
BbvI GCAGC 2 cut(s) 607, 877
BciT130I CCWGG 4 cut(s) 418, 627, 723, 801
BciVI GTATCC 2 cut(s) 7, 656
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 3 cut(s) 753, 754, 1158
BcoDI GTCTC 1 cut(s) 1341
BfaI CTAG 6 cut(s) 18, 56, 309, 404, 1086, 1427
BfmI CTRYAG 3 cut(s) 324, 1251, 1278
BfuI GTATCC 2 cut(s) 7, 656
BisI GCNGC 2 cut(s) 621, 891
BlnI CCTAGG 1 cut(s) 55
BlpI GCTNAGC 2 cut(s) 84, 450
BlsI GCNGC 2 cut(s) 622, 892
BmcAI AGTACT 2 cut(s) 149, 232
Bme1390I CCNGG 7 cut(s) 418, 627, 723, 753, 754, 801, 1158
Bme18I GGWCC 5 cut(s) 251, 265, 380, 851, 1433
BmeT110I CYCGRG 2 cut(s) 678, 752
BmgT120I GGNCC 6 cut(s) 251, 265, 284, 380, 851, 1433
BmiI GGNNCC 5 cut(s) 423, 750, 912, 1114, 1239
BmrFI CCNGG 7 cut(s) 418, 627, 723, 753, 754, 801, 1158
BmsI GCATC 5 cut(s) 450, 491, 676, 882, 949
BpmI CTGGAG 1 cut(s) 648
Bpu1102I GCTNAGC 2 cut(s) 84, 450
BpuEI CTTGAG 1 cut(s) 136
BpuMI CCSGG 3 cut(s) 753, 754, 1158
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 1341
BsaJI CCNNGG 7 cut(s) 55, 178, 417, 722, 752, 963, 1331
BsaWI WCCGGW 1 cut(s) 907
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 4 cut(s) 282, 592, 848, 1027
Bse1I ACTGG 2 cut(s) 145, 667
Bse3DI GCAATG 1 cut(s) 1439
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 4 cut(s) 418, 627, 723, 801
BseDI CCNNGG 7 cut(s) 55, 178, 417, 722, 752, 963, 1331
BseGI GGATG 3 cut(s) 139, 873, 964
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 282, 592, 848, 1027
BseMI GCAATG 1 cut(s) 1439
BseMII CTCAG 2 cut(s) 225, 1388
BseNI ACTGG 2 cut(s) 145, 667
BseXI GCAGC 2 cut(s) 607, 877
BsgI GTGCAG 1 cut(s) 606
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 3 cut(s) 286, 484, 1187
BshNI GGYRCC 1 cut(s) 1237
BsiEI CGRYCG 1 cut(s) 49
BsiHKCI CYCGRG 2 cut(s) 678, 752
BsiSI CCGG 4 cut(s) 593, 753, 908, 1158
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 4 cut(s) 282, 592, 848, 1027
BsmAI GTCTC 1 cut(s) 1341
BsmFI GGGAC 1 cut(s) 1008
BsnI GGCC 3 cut(s) 286, 484, 1187
Bso31I GGTCTC 1 cut(s) 1341
BsoBI CYCGRG 2 cut(s) 678, 752
Bsp143I GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
Bsp1720I GCTNAGC 2 cut(s) 84, 450
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 4 cut(s) 5, 1191, 1258, 1386
BspANI GGCC 3 cut(s) 286, 484, 1187
BspCNI CTCAG 2 cut(s) 226, 1387
BspHI TCATGA 2 cut(s) 927, 1060
BspLI GGNNCC 5 cut(s) 423, 750, 912, 1114, 1239
BspPI GGATC 7 cut(s) 416, 429, 541, 646, 905, 918, 995
BspT107I GGYRCC 1 cut(s) 1237
BspTNI GGTCTC 1 cut(s) 1341
BsrDI GCAATG 1 cut(s) 1439
BsrI ACTGG 2 cut(s) 145, 667
BssECI CCNNGG 7 cut(s) 55, 178, 417, 722, 752, 963, 1331
BssMI GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
BssT1I CCWWGG 3 cut(s) 55, 178, 1331
Bst2UI CCWGG 4 cut(s) 418, 627, 723, 801
Bst4CI ACNGT 1 cut(s) 1039
Bst6I CTCTTC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 482, 598
BstDEI CTNAG 4 cut(s) 84, 234, 450, 1374
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 3 cut(s) 139, 873, 964
BstKTI GATC 8 cut(s) 222, 424, 536, 641, 883, 913, 990, 1027
BstMAI GTCTC 1 cut(s) 1341
BstMBI GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 3 cut(s) 460, 602, 1199
BstNI CCWGG 4 cut(s) 418, 627, 723, 801
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 7 cut(s) 416, 625, 721, 751, 752, 799, 1156
BstSFI CTRYAG 3 cut(s) 324, 1251, 1278
BstV1I GCAGC 2 cut(s) 607, 877
BstX2I RGATCY 3 cut(s) 421, 910, 987
BstYI RGATCY 3 cut(s) 421, 910, 987
BsuI GTATCC 2 cut(s) 7, 656
BsuRI GGCC 3 cut(s) 286, 484, 1187
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 139, 873, 964
BtsIMutI CAGTG 1 cut(s) 1035
Cac8I GCNNGC 2 cut(s) 482, 598
CciI TCATGA 2 cut(s) 927, 1060
Cfr13I GGNCC 6 cut(s) 251, 265, 284, 380, 851, 1433
Cfr9I CCCGGG 1 cut(s) 752
CseI GACGC 1 cut(s) 455
Csp6I GTAC 6 cut(s) 142, 148, 192, 231, 568, 1328
CviQI GTAC 6 cut(s) 142, 148, 192, 231, 568, 1328
DdeI CTNAG 4 cut(s) 84, 234, 450, 1374
DpnI GATC 8 cut(s) 221, 423, 535, 640, 882, 912, 989, 1026
DpnII GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
DraI TTTAAA 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 3 cut(s) 1180, 1273, 1401
Eco130I CCWWGG 3 cut(s) 55, 178, 1331
Eco31I GGTCTC 1 cut(s) 1341
Eco47I GGWCC 5 cut(s) 251, 265, 380, 851, 1433
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 678, 752
EcoO109I RGGNCCY 2 cut(s) 265, 1433
EcoRI GAATTC 1 cut(s) 1293
EcoRII CCWGG 4 cut(s) 416, 625, 721, 799
EcoT14I CCWWGG 3 cut(s) 55, 178, 1331
EcoT22I ATGCAT 2 cut(s) 465, 745
ErhI CCWWGG 3 cut(s) 55, 178, 1331
FalI AAGNNNNNCTT 6 cut(s) 72, 104, 469, 501, 589, 621
FaqI GGGAC 1 cut(s) 1008
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 2 cut(s) 621, 891
FokI GGATG 3 cut(s) 146, 860, 971
Fsp4HI GCNGC 2 cut(s) 621, 891
FspBI CTAG 6 cut(s) 18, 56, 309, 404, 1086, 1427
GluI GCNGC 2 cut(s) 621, 891
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 3 cut(s) 286, 484, 1187
HapII CCGG 4 cut(s) 593, 753, 908, 1158
HgaI GACGC 1 cut(s) 455
HindIII AAGCTT 3 cut(s) 86, 824, 1136
HinfI GANTC 5 cut(s) 676, 757, 810, 1126, 1404
HpaII CCGG 4 cut(s) 593, 753, 908, 1158
HphI GGTGA 3 cut(s) 262, 368, 484
Hpy166II GTNNAC 2 cut(s) 251, 1033
Hpy188I TCNGA 9 cut(s) 13, 258, 523, 958, 967, 1112, 1175, 1377, 1424
Hpy188III TCNNGA 4 cut(s) 77, 784, 928, 1061
Hpy8I GTNNAC 2 cut(s) 251, 1033
Hpy99I CGWCG 2 cut(s) 292, 449
HpyAV CCTTC 3 cut(s) 18, 1343, 1424
HpyCH4III ACNGT 1 cut(s) 1039
HpyCH4IV ACGT 2 cut(s) 997, 1101
HpyCH4V TGCA 7 cut(s) 101, 463, 623, 689, 743, 1202, 1345
HpyF10VI GCNNNNNNNGC 3 cut(s) 460, 602, 1199
HpyF3I CTNAG 4 cut(s) 84, 234, 450, 1374
HpySE526I ACGT 2 cut(s) 997, 1101
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
LmnI GCTCC 3 cut(s) 523, 1159, 1176
Lsp1109I GCAGC 2 cut(s) 607, 877
LweI GCATC 5 cut(s) 450, 491, 676, 882, 949
MaeI CTAG 6 cut(s) 18, 56, 309, 404, 1086, 1427
MaeII ACGT 2 cut(s) 997, 1101
MaeIII GTNAC 1 cut(s) 1081
MalI GATC 8 cut(s) 221, 423, 535, 640, 882, 912, 989, 1026
MboI GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
MboII GAAGA 2 cut(s) 33, 479
MflI RGATCY 3 cut(s) 421, 910, 987
MluCI AATT 7 cut(s) 344, 490, 547, 733, 846, 1130, 1293
MlyI GAGTC 2 cut(s) 670, 1413
MmeI TCCRAC 4 cut(s) 798, 944, 1090, 1188
Mph1103I ATGCAT 2 cut(s) 465, 745
MseI TTAA 7 cut(s) 92, 399, 573, 795, 1134, 1221, 1323
MslI CAYNNNNRTG 2 cut(s) 957, 1444
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 4 cut(s) 593, 753, 908, 1158
MspR9I CCNGG 7 cut(s) 418, 627, 723, 753, 754, 801, 1158
MvaI CCWGG 4 cut(s) 418, 627, 723, 801
MwoI GCNNNNNNNGC 3 cut(s) 460, 602, 1199
NciI CCSGG 3 cut(s) 753, 754, 1158
NcoI CCATGG 1 cut(s) 178
NdeII GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
NlaIV GGNNCC 5 cut(s) 423, 750, 912, 1114, 1239
NmeAIII GCCGAG 2 cut(s) 581, 919
NmuCI GTSAC 1 cut(s) 1081
NsiI ATGCAT 2 cut(s) 465, 745
NspI RCATGY 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 678
PagI TCATGA 2 cut(s) 927, 1060
PciI ACATGT 1 cut(s) 712
PfeI GAWTC 3 cut(s) 757, 810, 1126
PflFI GACNNNGTC 1 cut(s) 701
PflMI CCANNNNNTGG 2 cut(s) 848, 1027
PfoI TCCNGGA 1 cut(s) 1156
PkrI GCNGC 2 cut(s) 622, 892
PleI GAGTC 2 cut(s) 670, 1412
PpsI GAGTC 2 cut(s) 670, 1412
PpuMI RGGWCCY 2 cut(s) 265, 1433
PscI ACATGT 1 cut(s) 712
PsiI TTATAA 1 cut(s) 1167
Psp5II RGGWCCY 2 cut(s) 265, 1433
Psp6I CCWGG 4 cut(s) 416, 625, 721, 799
PspGI CCWGG 4 cut(s) 416, 625, 721, 799
PspN4I GGNNCC 5 cut(s) 423, 750, 912, 1114, 1239
PspPI GGNCC 6 cut(s) 251, 265, 284, 380, 851, 1433
PspPPI RGGWCCY 2 cut(s) 265, 1433
PspXI VCTCGAGB 1 cut(s) 678
PsuI RGATCY 3 cut(s) 421, 910, 987
PsyI GACNNNGTC 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 6 cut(s) 143, 149, 193, 232, 569, 1329
RsaNI GTAC 6 cut(s) 142, 148, 192, 231, 568, 1328
RseI CAYNNNNRTG 2 cut(s) 957, 1444
SaqAI TTAA 7 cut(s) 92, 399, 573, 795, 1134, 1221, 1323
SatI GCNGC 2 cut(s) 621, 891
Sau3AI GATC 8 cut(s) 219, 421, 533, 638, 880, 910, 987, 1024
Sau96I GGNCC 6 cut(s) 251, 265, 284, 380, 851, 1433
ScaI AGTACT 2 cut(s) 149, 232
SchI GAGTC 2 cut(s) 670, 1413
ScrFI CCNGG 7 cut(s) 418, 627, 723, 753, 754, 801, 1158
SfaNI GCATC 5 cut(s) 450, 491, 676, 882, 949
SfcI CTRYAG 3 cut(s) 324, 1251, 1278
Sfr274I CTCGAG 1 cut(s) 678
SinI GGWCC 5 cut(s) 251, 265, 380, 851, 1433
SlaI CTCGAG 1 cut(s) 678
SmaI CCCGGG 1 cut(s) 754
SmiMI CAYNNNNRTG 2 cut(s) 957, 1444
SmlI CTYRAG 2 cut(s) 151, 678
SmoI CTYRAG 2 cut(s) 151, 678
Sse9I AATT 7 cut(s) 344, 490, 547, 733, 846, 1130, 1293
SsiI CCGC 4 cut(s) 5, 1191, 1258, 1386
SspMI CTAG 6 cut(s) 18, 56, 309, 404, 1086, 1427
StyD4I CCNGG 7 cut(s) 416, 625, 721, 751, 752, 799, 1156
StyI CCWWGG 3 cut(s) 55, 178, 1331
TaaI ACNGT 1 cut(s) 1039
TaiI ACGT 2 cut(s) 1000, 1104
TaqI TCGA 9 cut(s) 499, 679, 718, 783, 808, 813, 883, 1216, 1317
TaqII GACCGA 1 cut(s) 63
TasI AATT 7 cut(s) 344, 490, 547, 733, 846, 1130, 1293
TatI WGTACW 2 cut(s) 147, 230
TfiI GAWTC 3 cut(s) 757, 810, 1126
Tru1I TTAA 7 cut(s) 92, 399, 573, 795, 1134, 1221, 1323
Tru9I TTAA 7 cut(s) 92, 399, 573, 795, 1134, 1221, 1323
TscAI CASTG 1 cut(s) 1042
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 2 cut(s) 620, 890
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 6 cut(s) 19, 110, 480, 944, 1286, 1362
TspMI CCCGGG 1 cut(s) 752
TspRI CASTG 1 cut(s) 1042
Tth111I GACNNNGTC 1 cut(s) 701
Van91I CCANNNNNTGG 2 cut(s) 848, 1027
VpaK11BI GGWCC 5 cut(s) 251, 265, 380, 851, 1433
XapI RAATTY 3 cut(s) 344, 547, 1293
XceI RCATGY 1 cut(s) 716
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 678
XmaI CCCGGG 1 cut(s) 752
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 6 cut(s) 18, 56, 309, 404, 1086, 1427
ZrmI AGTACT 2 cut(s) 149, 232
Zsp2I ATGCAT 2 cut(s) 465, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.