FvH4_5g16800

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014744
Physical Location & Seq
Reverse (-)
9604620 .. 9610087
5468 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16800.t2

Sequence Viewer

Length: 789 bp
ATGGATAGCTTGGACTATCTGCTGGTACCAGCAGGTCTGTTTGTGTTGGGGTTGTACCATGTTTGGCTAATCATCACAGTTCTGCGCAATCCCATACGAACTGTCATTGGCGTCAATGCTCACACTCGTCAACAATGGGTCTTCTCCATGATGACTGACCCTTTGAAGAATGGTGTTTTGATTATACAAACTCTACGCAACAACATAATGGCATCGACACTTCTGGCAACAACAGAAATTACTCTTTGTTCTGTGATCGTTGTACTTGTGAGCAGCCAATCCAGCTCCAGTAGCACTGTATCATTACACTTAGTTTACTCTGATTATACTCCCTCCTTAACTTCAATCAAGTACTTGTGCATTTTGCTTTGCTTTCTTGTTGCATTTCTCTGCAATGTGCAGTCTACTAGATATTATGCCCATGTCAGCTTCTTGGGTACTGTCCCAACATGGAAAAACAAGCCAGGTTACATTGAGTATGTTGCAGCAACTTTGAACCGAGGATATTACTTTTGGGCCCTGGGATTGCGAGCATTCTACCTCTCCATTCCTCTCTTTCTCTGGATCTTTGGGCCGATACCTATGTTTGTCAGTTGCTGTGTAATGTCAGTTGTTCTCTATTTCTTGGACACAACCTCCAGCTTTGCACAACTTATTCACACCCAATTGTTCAGGGAGGAAGAAAAAACCGATAATGTGGAGCCAATTGCTCAATCACTGAACAATTCTGATCGTGAGAACTTCTTCCAACTTCGTGATCCTCTAGTCACCAGGTCCGGCTGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

263

Amino Acids

29.76

Weight (kDa)

7.62

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 7 - 213 7.4e-64 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 23
Acc65I GGTACC 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 404
AclWI GGATC 2 cut(s) 572, 752
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 5 cut(s) 27, 56, 264, 353, 439
AgsI TTSAA 3 cut(s) 166, 345, 496
AjnI CCWGG 3 cut(s) 463, 519, 770
AluBI AGCT 4 cut(s) 9, 285, 429, 642
AluI AGCT 4 cut(s) 9, 285, 429, 642
AlwI GGATC 2 cut(s) 572, 752
AlwNI CAGNNNCTG 1 cut(s) 597
AoxI GGCC 2 cut(s) 516, 572
ApaI GGGCCC 1 cut(s) 520
ApeKI GCWGC 2 cut(s) 273, 485
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
Asp700I GAANNNNTTC 1 cut(s) 743
Asp718I GGTACC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 4 cut(s) 516, 517, 572, 774
AsuHPI GGTGA 1 cut(s) 760
AvaII GGWCC 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 520
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 285, 497
BciT130I CCWGG 3 cut(s) 465, 521, 772
BfaI CTAG 2 cut(s) 408, 764
BfuAI ACCTGC 1 cut(s) 23
BisI GCNGC 2 cut(s) 274, 486
BlsI GCNGC 2 cut(s) 275, 487
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 3 cut(s) 465, 521, 772
Bme18I GGWCC 1 cut(s) 774
BmgT120I GGNCC 4 cut(s) 516, 517, 572, 774
BmiI GGNNCC 3 cut(s) 27, 518, 702
BmrFI CCNGG 3 cut(s) 465, 521, 772
BmsI GCATC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 2 cut(s) 271, 622
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 499, 519, 520
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 3 cut(s) 465, 521, 772
BseDI CCNNGG 3 cut(s) 499, 519, 520
BseMI GCAATG 1 cut(s) 400
BseNI ACTGG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 2 cut(s) 285, 497
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 2 cut(s) 518, 574
BshNI GGYRCC 1 cut(s) 25
BsiSI CCGG 1 cut(s) 777
BslFI GGGAC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 2 cut(s) 518, 574
Bsp120I GGGCCC 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 4 cut(s) 255, 564, 730, 757
BspANI GGCC 2 cut(s) 518, 574
BspLI GGNNCC 3 cut(s) 27, 518, 702
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 572, 752
BspT107I GGYRCC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 3 cut(s) 499, 519, 520
BssMI GATC 4 cut(s) 255, 564, 730, 757
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 3 cut(s) 465, 521, 772
Bst4CI ACNGT 4 cut(s) 79, 103, 298, 442
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 531, 782
BstDEI CTNAG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 4 cut(s) 258, 567, 733, 760
BstMBI GATC 4 cut(s) 255, 564, 730, 757
BstMWI GCNNNNNNNGC 2 cut(s) 282, 291
BstNI CCWGG 3 cut(s) 465, 521, 772
BstSCI CCNGG 3 cut(s) 463, 519, 770
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 2 cut(s) 285, 497
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 2 cut(s) 518, 574
BtsIMutI CAGTG 2 cut(s) 294, 716
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 531, 782
CaiI CAGNNNCTG 1 cut(s) 597
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 4 cut(s) 516, 517, 572, 774
CseI GACGC 1 cut(s) 100
CsiI ACCWGGT 1 cut(s) 770
Csp6I GTAC 5 cut(s) 26, 55, 263, 352, 438
CviAII CATG 4 cut(s) 59, 148, 422, 450
CviQI GTAC 5 cut(s) 26, 55, 263, 352, 438
DdeI CTNAG 1 cut(s) 310
DpnI GATC 4 cut(s) 257, 566, 732, 759
DpnII GATC 4 cut(s) 255, 564, 730, 757
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 517
EcoRII CCWGG 3 cut(s) 463, 519, 770
EcoT38I GRGCYC 1 cut(s) 520
FaeI CATG 4 cut(s) 62, 151, 425, 453
FaqI GGGAC 1 cut(s) 428
FatI CATG 4 cut(s) 58, 147, 421, 449
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 2 cut(s) 274, 486
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 2 cut(s) 274, 486
FspBI CTAG 2 cut(s) 408, 764
FspI TGCGCA 1 cut(s) 86
GlaI GCGC 1 cut(s) 86
GluI GCNGC 2 cut(s) 274, 486
GsuI CTGGAG 2 cut(s) 271, 622
HaeIII GGCC 2 cut(s) 518, 574
HapII CCGG 1 cut(s) 777
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 4 cut(s) 62, 151, 425, 453
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 1 cut(s) 131
HindII GTYRAC 1 cut(s) 131
HpaII CCGG 1 cut(s) 777
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 3 cut(s) 131, 316, 405
Hpy188I TCNGA 2 cut(s) 322, 730
Hpy188III TCNNGA 3 cut(s) 562, 734, 755
Hpy8I GTNNAC 3 cut(s) 131, 316, 405
HpyCH4III ACNGT 4 cut(s) 79, 103, 298, 442
HpyCH4V TGCA 6 cut(s) 360, 383, 393, 400, 485, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 291
HpyF3I CTNAG 1 cut(s) 310
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 4 cut(s) 62, 151, 425, 453
HspAI GCGC 1 cut(s) 85
KpnI GGTACC 1 cut(s) 29
Kzo9I GATC 4 cut(s) 255, 564, 730, 757
LmnI GCTCC 2 cut(s) 290, 700
Lsp1109I GCAGC 2 cut(s) 285, 497
LweI GCATC 1 cut(s) 221
MabI ACCWGGT 1 cut(s) 770
MaeI CTAG 2 cut(s) 408, 764
MaeIII GTNAC 2 cut(s) 467, 766
MalI GATC 4 cut(s) 257, 566, 732, 759
MboI GATC 4 cut(s) 255, 564, 730, 757
MboII GAAGA 4 cut(s) 133, 178, 692, 736
MfeI CAATTG 2 cut(s) 665, 705
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 4 cut(s) 237, 665, 705, 724
MmeI TCCRAC 1 cut(s) 772
MnlI CCTC 7 cut(s) 343, 494, 551, 561, 646, 670, 771
MroXI GAANNNNTTC 1 cut(s) 743
MseI TTAA 1 cut(s) 338
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 3 cut(s) 465, 521, 772
MunI CAATTG 2 cut(s) 665, 705
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 3 cut(s) 465, 521, 772
MwoI GCNNNNNNNGC 2 cut(s) 282, 291
NdeII GATC 4 cut(s) 255, 564, 730, 757
NlaIII CATG 4 cut(s) 62, 151, 425, 453
NlaIV GGNNCC 3 cut(s) 27, 518, 702
NmuCI GTSAC 1 cut(s) 766
NsbI TGCGCA 1 cut(s) 86
PasI CCCWGGG 1 cut(s) 520
PctI GAATGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 743
PkrI GCNGC 2 cut(s) 275, 487
Psp6I CCWGG 3 cut(s) 463, 519, 770
PspGI CCWGG 3 cut(s) 463, 519, 770
PspN4I GGNNCC 3 cut(s) 27, 518, 702
PspOMI GGGCCC 1 cut(s) 516
PspPI GGNCC 4 cut(s) 516, 517, 572, 774
PstNI CAGNNNCTG 1 cut(s) 597
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 5 cut(s) 27, 56, 264, 353, 439
RsaNI GTAC 5 cut(s) 26, 55, 263, 352, 438
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 2 cut(s) 274, 486
Sau3AI GATC 4 cut(s) 255, 564, 730, 757
Sau96I GGNCC 4 cut(s) 516, 517, 572, 774
ScaI AGTACT 1 cut(s) 353
ScrFI CCNGG 3 cut(s) 465, 521, 772
SduI GDGCHC 1 cut(s) 520
SexAI ACCWGGT 1 cut(s) 770
SfaNI GCATC 1 cut(s) 221
SinI GGWCC 1 cut(s) 774
Sse9I AATT 4 cut(s) 237, 665, 705, 724
SspMI CTAG 2 cut(s) 408, 764
StyD4I CCNGG 3 cut(s) 463, 519, 770
TaaI ACNGT 4 cut(s) 79, 103, 298, 442
TaqI TCGA 1 cut(s) 215
TasI AATT 4 cut(s) 237, 665, 705, 724
TatI WGTACW 2 cut(s) 262, 351
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 2 cut(s) 301, 723
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 2 cut(s) 273, 485
Tsp45I GTSAC 1 cut(s) 766
TspRI CASTG 2 cut(s) 301, 723
VpaK11BI GGWCC 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 2 cut(s) 408, 764
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.