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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G37070 AT2G37070 AT3G53320 |
| fragaria_vesca | FvH4_6g15330 FvH4_6g15330 FvH4_6g15330 |
| malus_domestica | MD04G1104400.v1.1 MD12G1124300.v1.1 |
| prunus_persica | Prupe.6G241300_v2.0.a1 Prupe.6G241300_v2.0.a1 Prupe.6G241300_v2.0.a1 |
| pyrus_communis | pycom04g09930 pycom12g12270 |
| rosa_chinensis | RchiOBHm_Chr3g0468801 |
| rosa_laevigata | RLG00000024408 |
| rosa_multiflora | Rmu_sc0001639.1_g000024 |
| rosa_roxburghii | Rroxscaffold_6G00412550 |
| rosa_rugosa | Rorug03G0096600 |
| rosa_samantha | Rh3AG146300 Rh3BG168900 Rh3DG167200 |
| rosa_wichuraiana | Rw3G013760 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1231 |
| Acc36I | ACCTGC | 3 cut(s) | 1231, 1645, 2242 |
| AccB1I | GGYRCC | 1 cut(s) | 854 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1106, 1484 |
| AccI | GTMKAC | 1 cut(s) | 2093 |
| AccIII | TCCGGA | 1 cut(s) | 175 |
| AciI | CCGC | 4 cut(s) | 170, 518, 1227, 2180 |
| AclWI | GGATC | 4 cut(s) | 1356, 1392, 1509, 1522 |
| AcsI | RAATTY | 1 cut(s) | 390 |
| AcuI | CTGAAG | 4 cut(s) | 1698, 1875, 1970, 2063 |
| AcyI | GRCGYC | 1 cut(s) | 34 |
| AfaI | GTAC | 5 cut(s) | 568, 579, 690, 1042, 1803 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 1106, 1345, 1484, 1574, 1612, 1814, 2084 |
| AhlI | ACTAGT | 1 cut(s) | 2096 |
| AjnI | CCWGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| AleI | CACNNNNGTG | 1 cut(s) | 981 |
| Alw21I | GWGCWC | 2 cut(s) | 192, 1435 |
| Alw26I | GTCTC | 3 cut(s) | 87, 1364, 2331 |
| Alw44I | GTGCAC | 1 cut(s) | 1431 |
| AlwI | GGATC | 4 cut(s) | 1356, 1392, 1509, 1522 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1721 |
| Aor13HI | TCCGGA | 1 cut(s) | 175 |
| AoxI | GGCC | 3 cut(s) | 456, 659, 1603 |
| ApaLI | GTGCAC | 1 cut(s) | 1431 |
| ApeKI | GCWGC | 9 cut(s) | 149, 152, 824, 1053, 1204, 1742, 1970, 2063, 2168 |
| ApoI | RAATTY | 1 cut(s) | 390 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2216 |
| AspLEI | GCGC | 1 cut(s) | 149 |
| AspS9I | GGNCC | 4 cut(s) | 302, 399, 1245, 1603 |
| AsuHPI | GGTGA | 8 cut(s) | 79, 1089, 1119, 1212, 1417, 1467, 1513, 2333 |
| AsuNHI | GCTAGC | 1 cut(s) | 1265 |
| AvaII | GGWCC | 3 cut(s) | 302, 399, 1245 |
| BaeGI | GKGCMC | 3 cut(s) | 859, 948, 1435 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 550, 583 |
| BamHI | GGATCC | 1 cut(s) | 1514 |
| BanI | GGYRCC | 1 cut(s) | 854 |
| BanII | GRGCYC | 2 cut(s) | 192, 910 |
| BbsI | GAAGAC | 3 cut(s) | 564, 680, 2209 |
| Bbv12I | GWGCWC | 2 cut(s) | 192, 1435 |
| BbvCI | CCTCAGC | 2 cut(s) | 38, 321 |
| BbvI | GCAGC | 9 cut(s) | 136, 139, 836, 1065, 1191, 1729, 1957, 2050, 2180 |
| BccI | CCATC | 6 cut(s) | 338, 967, 1108, 1480, 1490, 2078 |
| BceAI | ACGGC | 1 cut(s) | 1704 |
| BciT130I | CCWGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| BciVI | GTATCC | 1 cut(s) | 871 |
| BcoDI | GTCTC | 3 cut(s) | 87, 1364, 2331 |
| BcuI | ACTAGT | 1 cut(s) | 2096 |
| BfaI | CTAG | 7 cut(s) | 498, 723, 816, 1266, 1557, 1641, 2097 |
| BfmI | CTRYAG | 1 cut(s) | 1299 |
| BfoI | RGCGCY | 1 cut(s) | 150 |
| BfuAI | ACCTGC | 3 cut(s) | 1231, 1645, 2242 |
| BfuI | GTATCC | 1 cut(s) | 871 |
| BglII | AGATCT | 1 cut(s) | 494 |
| BlpI | GCTNAGC | 2 cut(s) | 820, 2304 |
| BmcAI | AGTACT | 1 cut(s) | 1042 |
| Bme1390I | CCNGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| Bme18I | GGWCC | 3 cut(s) | 302, 399, 1245 |
| BmgT120I | GGNCC | 4 cut(s) | 302, 399, 1245, 1603 |
| BmiI | GGNNCC | 7 cut(s) | 174, 228, 856, 909, 1516, 1604, 2178 |
| BmrFI | CCNGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| BmsI | GCATC | 6 cut(s) | 157, 307, 515, 716, 1055, 2116 |
| BmtI | GCTAGC | 1 cut(s) | 1269 |
| BpiI | GAAGAC | 3 cut(s) | 564, 680, 2209 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1254, 1286, 1765, 1797, 2099, 2131 |
| BpmI | CTGGAG | 2 cut(s) | 894, 1063 |
| Bpu10I | CCTNAGC | 2 cut(s) | 38, 321 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 820, 2304 |
| BpuEI | CTTGAG | 5 cut(s) | 152, 206, 624, 1615, 2284 |
| Bsa29I | ATCGAT | 1 cut(s) | 2082 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1471 |
| BsaHI | GRCGYC | 1 cut(s) | 34 |
| BsaJI | CCNNGG | 5 cut(s) | 374, 698, 1142, 1339, 1401 |
| BsaWI | WCCGGW | 2 cut(s) | 89, 175 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 556, 586 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 1106, 1345, 1484, 1574, 1612, 1814, 2084 |
| Bse1I | ACTGG | 5 cut(s) | 230, 304, 901, 911, 1387 |
| Bse3DI | GCAATG | 1 cut(s) | 508 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1471 |
| BseAI | TCCGGA | 1 cut(s) | 175 |
| BseBI | CCWGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| BseCI | ATCGAT | 1 cut(s) | 2082 |
| BseDI | CCNNGG | 5 cut(s) | 374, 698, 1142, 1339, 1401 |
| BseGI | GGATG | 7 cut(s) | 372, 526, 1046, 1100, 1543, 1804, 1843 |
| BseJI | GATNNNNATC | 1 cut(s) | 1471 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 1106, 1345, 1484, 1574, 1612, 1814, 2084 |
| BseMI | GCAATG | 1 cut(s) | 508 |
| BseMII | CTCAG | 7 cut(s) | 52, 120, 312, 834, 1263, 1388, 2318 |
| BseNI | ACTGG | 5 cut(s) | 230, 304, 901, 911, 1387 |
| BseRI | GAGGAG | 2 cut(s) | 338, 1428 |
| BseSI | GKGCMC | 3 cut(s) | 859, 948, 1435 |
| BseXI | GCAGC | 9 cut(s) | 136, 139, 836, 1065, 1191, 1729, 1957, 2050, 2180 |
| BseYI | CCCAGC | 2 cut(s) | 948, 1621 |
| BshFI | GGCC | 3 cut(s) | 458, 661, 1605 |
| BshNI | GGYRCC | 1 cut(s) | 854 |
| BshVI | ATCGAT | 1 cut(s) | 2082 |
| BsiHKAI | GWGCWC | 2 cut(s) | 192, 1435 |
| BsiSI | CCGG | 2 cut(s) | 90, 176 |
| BslFI | GGGAC | 1 cut(s) | 1591 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 1106, 1345, 1484, 1574, 1612, 1814, 2084 |
| BsmAI | GTCTC | 3 cut(s) | 87, 1364, 2331 |
| BsmFI | GGGAC | 1 cut(s) | 1591 |
| BsmI | GAATGC | 2 cut(s) | 1414, 1729 |
| BsnI | GGCC | 3 cut(s) | 458, 661, 1605 |
| Bsp1286I | GDGCHC | 5 cut(s) | 192, 859, 910, 948, 1435 |
| Bsp13I | TCCGGA | 1 cut(s) | 175 |
| Bsp1407I | TGTACA | 1 cut(s) | 688 |
| Bsp143I | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 820, 2304 |
| Bsp19I | CCATGG | 1 cut(s) | 1339 |
| BspACI | CCGC | 4 cut(s) | 170, 518, 1227, 2180 |
| BspANI | GGCC | 3 cut(s) | 458, 661, 1605 |
| BspCNI | CTCAG | 7 cut(s) | 51, 119, 313, 833, 1262, 1387, 2317 |
| BspDI | ATCGAT | 1 cut(s) | 2082 |
| BspEI | TCCGGA | 1 cut(s) | 175 |
| BspHI | TCATGA | 2 cut(s) | 234, 2257 |
| BspLI | GGNNCC | 7 cut(s) | 174, 228, 856, 909, 1516, 1604, 2178 |
| BspMI | ACCTGC | 3 cut(s) | 1231, 1645, 2242 |
| BspOI | GCTAGC | 1 cut(s) | 1269 |
| BspPI | GGATC | 4 cut(s) | 1356, 1392, 1509, 1522 |
| BspQI | GCTCTTC | 2 cut(s) | 1766, 2241 |
| BspT107I | GGYRCC | 1 cut(s) | 854 |
| BsrDI | GCAATG | 1 cut(s) | 508 |
| BsrGI | TGTACA | 1 cut(s) | 688 |
| BsrI | ACTGG | 5 cut(s) | 230, 304, 901, 911, 1387 |
| BssECI | CCNNGG | 5 cut(s) | 374, 698, 1142, 1339, 1401 |
| BssMI | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| BssNI | GRCGYC | 1 cut(s) | 34 |
| BssT1I | CCWWGG | 1 cut(s) | 1339 |
| Bst2UI | CCWGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| Bst4CI | ACNGT | 7 cut(s) | 287, 744, 883, 1360, 1588, 1648, 1892 |
| Bst6I | CTCTTC | 3 cut(s) | 239, 1766, 2241 |
| BstACI | GRCGYC | 1 cut(s) | 34 |
| BstAUI | TGTACA | 1 cut(s) | 688 |
| BstC8I | GCNNGC | 3 cut(s) | 663, 717, 1267 |
| BstDSI | CCRYGG | 2 cut(s) | 1339, 1401 |
| BstF5I | GGATG | 7 cut(s) | 372, 526, 1046, 1100, 1543, 1804, 1843 |
| BstH2I | RGCGCY | 1 cut(s) | 150 |
| BstHHI | GCGC | 1 cut(s) | 149 |
| BstKTI | GATC | 8 cut(s) | 497, 1281, 1323, 1351, 1400, 1517, 1933, 2026 |
| BstMAI | GTCTC | 3 cut(s) | 87, 1364, 2331 |
| BstMBI | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 25, 992, 1726 |
| BstNI | CCWGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| BstSCI | CCNGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| BstSFI | CTRYAG | 1 cut(s) | 1299 |
| BstSLI | GKGCMC | 3 cut(s) | 859, 948, 1435 |
| BstV1I | GCAGC | 9 cut(s) | 136, 139, 836, 1065, 1191, 1729, 1957, 2050, 2180 |
| BstV2I | GAAGAC | 3 cut(s) | 564, 680, 2209 |
| BstX2I | RGATCY | 4 cut(s) | 494, 1348, 1397, 1514 |
| BstYI | RGATCY | 4 cut(s) | 494, 1348, 1397, 1514 |
| Bsu15I | ATCGAT | 1 cut(s) | 2082 |
| BsuI | GTATCC | 1 cut(s) | 871 |
| BsuRI | GGCC | 3 cut(s) | 458, 661, 1605 |
| BsuTUI | ATCGAT | 1 cut(s) | 2082 |
| BtgI | CCRYGG | 2 cut(s) | 1339, 1401 |
| BtsCI | GGATG | 7 cut(s) | 372, 526, 1046, 1100, 1543, 1804, 1843 |
| BtsI | GCAGTG | 2 cut(s) | 1914, 2007 |
| BtsIMutI | CAGTG | 8 cut(s) | 292, 297, 402, 918, 1243, 1722, 1914, 2007 |
| BveI | ACCTGC | 3 cut(s) | 1231, 1645, 2242 |
| Cac8I | GCNNGC | 3 cut(s) | 663, 717, 1267 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1721 |
| CciI | TCATGA | 2 cut(s) | 234, 2257 |
| CfoI | GCGC | 1 cut(s) | 149 |
| Cfr13I | GGNCC | 4 cut(s) | 302, 399, 1245, 1603 |
| ClaI | ATCGAT | 1 cut(s) | 2082 |
| CseI | GACGC | 3 cut(s) | 42, 448, 1319 |
| Csp6I | GTAC | 5 cut(s) | 567, 578, 689, 1041, 1802 |
| CviAII | CATG | 5 cut(s) | 235, 337, 1340, 1367, 2258 |
| CviQI | GTAC | 5 cut(s) | 567, 578, 689, 1041, 1802 |
| DpnI | GATC | 8 cut(s) | 496, 1280, 1322, 1350, 1399, 1516, 1932, 2025 |
| DpnII | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| Eam1104I | CTCTTC | 3 cut(s) | 239, 1766, 2241 |
| EarI | CTCTTC | 3 cut(s) | 239, 1766, 2241 |
| Ecl136II | GAGCTC | 1 cut(s) | 190 |
| Eco130I | CCWWGG | 1 cut(s) | 1339 |
| Eco147I | AGGCCT | 1 cut(s) | 661 |
| Eco24I | GRGCYC | 2 cut(s) | 192, 910 |
| Eco47I | GGWCC | 3 cut(s) | 302, 399, 1245 |
| Eco53kI | GAGCTC | 1 cut(s) | 190 |
| Eco57I | CTGAAG | 4 cut(s) | 1698, 1875, 1970, 2063 |
| EcoICRI | GAGCTC | 1 cut(s) | 190 |
| EcoRII | CCWGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| EcoT14I | CCWWGG | 1 cut(s) | 1339 |
| EcoT22I | ATGCAT | 1 cut(s) | 508 |
| EcoT38I | GRGCYC | 2 cut(s) | 192, 910 |
| ErhI | CCWWGG | 1 cut(s) | 1339 |
| FaeI | CATG | 5 cut(s) | 238, 340, 1343, 1370, 2261 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 245, 277, 262, 294, 418, 450, 760, 792 |
| FaqI | GGGAC | 1 cut(s) | 1591 |
| FatI | CATG | 5 cut(s) | 234, 336, 1339, 1366, 2257 |
| FblI | GTMKAC | 1 cut(s) | 2093 |
| FokI | GGATG | 7 cut(s) | 379, 533, 1033, 1087, 1530, 1791, 1850 |
| FriOI | GRGCYC | 2 cut(s) | 192, 910 |
| FspBI | CTAG | 7 cut(s) | 498, 723, 816, 1266, 1557, 1641, 2097 |
| GlaI | GCGC | 1 cut(s) | 148 |
| GsaI | CCCAGC | 2 cut(s) | 952, 1625 |
| GsuI | CTGGAG | 2 cut(s) | 894, 1063 |
| HaeII | RGCGCY | 1 cut(s) | 150 |
| HaeIII | GGCC | 3 cut(s) | 458, 661, 1605 |
| HapII | CCGG | 2 cut(s) | 90, 176 |
| HgaI | GACGC | 3 cut(s) | 42, 448, 1319 |
| HhaI | GCGC | 1 cut(s) | 149 |
| Hin1I | GRCGYC | 1 cut(s) | 34 |
| Hin1II | CATG | 5 cut(s) | 238, 340, 1343, 1370, 2261 |
| Hin6I | GCGC | 1 cut(s) | 147 |
| HinP1I | GCGC | 1 cut(s) | 147 |
| HincII | GTYRAC | 1 cut(s) | 121 |
| HindII | GTYRAC | 1 cut(s) | 121 |
| HpaII | CCGG | 2 cut(s) | 90, 176 |
| HphI | GGTGA | 8 cut(s) | 79, 1089, 1119, 1212, 1417, 1467, 1513, 2333 |
| Hpy166II | GTNNAC | 4 cut(s) | 121, 402, 1433, 2094 |
| Hpy188I | TCNGA | 9 cut(s) | 13, 109, 217, 1252, 1320, 1927, 2020, 2125, 2203 |
| Hpy8I | GTNNAC | 4 cut(s) | 121, 402, 1433, 2094 |
| Hpy99I | CGWCG | 1 cut(s) | 53 |
| HpyCH4III | ACNGT | 7 cut(s) | 287, 744, 883, 1360, 1588, 1648, 1892 |
| HpyCH4IV | ACGT | 2 cut(s) | 51, 2133 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 25, 992, 1726 |
| HpySE526I | ACGT | 2 cut(s) | 51, 2133 |
| Hsp92I | GRCGYC | 1 cut(s) | 34 |
| Hsp92II | CATG | 5 cut(s) | 238, 340, 1343, 1370, 2261 |
| HspAI | GCGC | 1 cut(s) | 147 |
| Kpn2I | TCCGGA | 1 cut(s) | 175 |
| Kzo9I | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| LguI | GCTCTTC | 2 cut(s) | 1766, 2241 |
| LmnI | GCTCC | 5 cut(s) | 325, 913, 1395, 1879, 2279 |
| Lsp1109I | GCAGC | 9 cut(s) | 136, 139, 836, 1065, 1191, 1729, 1957, 2050, 2180 |
| LweI | GCATC | 6 cut(s) | 157, 307, 515, 716, 1055, 2116 |
| MaeI | CTAG | 7 cut(s) | 498, 723, 816, 1266, 1557, 1641, 2097 |
| MaeII | ACGT | 2 cut(s) | 51, 2133 |
| MaeIII | GTNAC | 5 cut(s) | 369, 1009, 1195, 1978, 2071 |
| MalI | GATC | 8 cut(s) | 496, 1280, 1322, 1350, 1399, 1516, 1932, 2025 |
| MboI | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| MfeI | CAATTG | 1 cut(s) | 1023 |
| MflI | RGATCY | 4 cut(s) | 494, 1348, 1397, 1514 |
| MhlI | GDGCHC | 5 cut(s) | 192, 859, 910, 948, 1435 |
| MlyI | GAGTC | 8 cut(s) | 74, 227, 1262, 1366, 1753, 2099, 2117, 2333 |
| MmeI | TCCRAC | 3 cut(s) | 1342, 1466, 2257 |
| Mph1103I | ATGCAT | 1 cut(s) | 508 |
| MroI | TCCGGA | 1 cut(s) | 175 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2216 |
| MseI | TTAA | 5 cut(s) | 531, 830, 929, 1287, 1736 |
| MslI | CAYNNNNRTG | 1 cut(s) | 981 |
| MspA1I | CMGCKG | 1 cut(s) | 1511 |
| MspI | CCGG | 2 cut(s) | 90, 176 |
| MspR9I | CCNGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| MunI | CAATTG | 1 cut(s) | 1023 |
| Mva1269I | GAATGC | 2 cut(s) | 1414, 1729 |
| MvaI | CCWGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 25, 992, 1726 |
| NcoI | CCATGG | 1 cut(s) | 1339 |
| NdeII | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| NheI | GCTAGC | 1 cut(s) | 1265 |
| NlaIII | CATG | 5 cut(s) | 238, 340, 1343, 1370, 2261 |
| NlaIV | GGNNCC | 7 cut(s) | 174, 228, 856, 909, 1516, 1604, 2178 |
| NmuCI | GTSAC | 3 cut(s) | 1195, 1978, 2071 |
| NsiI | ATGCAT | 1 cut(s) | 508 |
| OliI | CACNNNNGTG | 1 cut(s) | 981 |
| PagI | TCATGA | 2 cut(s) | 234, 2257 |
| PaqCI | CACCTGC | 1 cut(s) | 1231 |
| PceI | AGGCCT | 1 cut(s) | 661 |
| PciSI | GCTCTTC | 2 cut(s) | 1766, 2241 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 57 |
| PctI | GAATGC | 2 cut(s) | 1414, 1729 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2216 |
| PflFI | GACNNNGTC | 1 cut(s) | 116 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1106, 1484 |
| PfoI | TCCNGGA | 2 cut(s) | 1942, 2035 |
| PleI | GAGTC | 8 cut(s) | 74, 226, 1261, 1366, 1753, 2098, 2116, 2332 |
| PpsI | GAGTC | 8 cut(s) | 74, 226, 1261, 1366, 1753, 2098, 2116, 2332 |
| Psp124BI | GAGCTC | 1 cut(s) | 192 |
| Psp6I | CCWGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| PspFI | CCCAGC | 2 cut(s) | 948, 1621 |
| PspGI | CCWGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| PspN4I | GGNNCC | 7 cut(s) | 174, 228, 856, 909, 1516, 1604, 2178 |
| PspPI | GGNCC | 4 cut(s) | 302, 399, 1245, 1603 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1721 |
| PsuI | RGATCY | 4 cut(s) | 494, 1348, 1397, 1514 |
| PsyI | GACNNNGTC | 1 cut(s) | 116 |
| PvuII | CAGCTG | 1 cut(s) | 1511 |
| RsaI | GTAC | 5 cut(s) | 568, 579, 690, 1042, 1803 |
| RsaNI | GTAC | 5 cut(s) | 567, 578, 689, 1041, 1802 |
| RseI | CAYNNNNRTG | 1 cut(s) | 981 |
| SacI | GAGCTC | 1 cut(s) | 192 |
| SapI | GCTCTTC | 2 cut(s) | 1766, 2241 |
| SaqAI | TTAA | 5 cut(s) | 531, 830, 929, 1287, 1736 |
| Sau3AI | GATC | 8 cut(s) | 494, 1278, 1320, 1348, 1397, 1514, 1930, 2023 |
| Sau96I | GGNCC | 4 cut(s) | 302, 399, 1245, 1603 |
| ScaI | AGTACT | 1 cut(s) | 1042 |
| SchI | GAGTC | 8 cut(s) | 74, 227, 1262, 1366, 1753, 2099, 2117, 2333 |
| ScrFI | CCNGG | 6 cut(s) | 306, 375, 699, 1000, 1944, 2037 |
| SduI | GDGCHC | 5 cut(s) | 192, 859, 910, 948, 1435 |
| SfaNI | GCATC | 6 cut(s) | 157, 307, 515, 716, 1055, 2116 |
| SfcI | CTRYAG | 1 cut(s) | 1299 |
| SinI | GGWCC | 3 cut(s) | 302, 399, 1245 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 981 |
| SmlI | CTYRAG | 5 cut(s) | 131, 185, 639, 1594, 2263 |
| SmoI | CTYRAG | 5 cut(s) | 131, 185, 639, 1594, 2263 |
| SpeI | ACTAGT | 1 cut(s) | 2096 |
| SseBI | AGGCCT | 1 cut(s) | 661 |
| SsiI | CCGC | 4 cut(s) | 170, 518, 1227, 2180 |
| SspI | AATATT | 1 cut(s) | 529 |
| SspMI | CTAG | 7 cut(s) | 498, 723, 816, 1266, 1557, 1641, 2097 |
| SstI | GAGCTC | 1 cut(s) | 192 |
| StuI | AGGCCT | 1 cut(s) | 661 |
| StyD4I | CCNGG | 6 cut(s) | 304, 373, 697, 998, 1942, 2035 |
| StyI | CCWWGG | 1 cut(s) | 1339 |
| TaaI | ACNGT | 7 cut(s) | 287, 744, 883, 1360, 1588, 1648, 1892 |
| TaiI | ACGT | 2 cut(s) | 54, 2136 |
| TaqI | TCGA | 6 cut(s) | 48, 78, 444, 1132, 1272, 2082 |
| TatI | WGTACW | 4 cut(s) | 577, 688, 1040, 1801 |
| TauI | GCSGC | 1 cut(s) | 1229 |
| Tru1I | TTAA | 5 cut(s) | 531, 830, 929, 1287, 1736 |
| Tru9I | TTAA | 5 cut(s) | 531, 830, 929, 1287, 1736 |
| TscAI | CASTG | 8 cut(s) | 292, 304, 409, 918, 1243, 1722, 1921, 2014 |
| TseFI | GTSAC | 3 cut(s) | 1195, 1978, 2071 |
| TseI | GCWGC | 9 cut(s) | 149, 152, 824, 1053, 1204, 1742, 1970, 2063, 2168 |
| Tsp45I | GTSAC | 3 cut(s) | 1195, 1978, 2071 |
| TspDTI | ATGAA | 6 cut(s) | 210, 537, 1667, 1715, 1722, 2246 |
| TspGWI | ACGGA | 2 cut(s) | 1382, 1390 |
| TspRI | CASTG | 8 cut(s) | 292, 304, 409, 918, 1243, 1722, 1921, 2014 |
| Tth111I | GACNNNGTC | 1 cut(s) | 116 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1106, 1484 |
| VneI | GTGCAC | 1 cut(s) | 1431 |
| VpaK11BI | GGWCC | 3 cut(s) | 302, 399, 1245 |
| XapI | RAATTY | 1 cut(s) | 390 |
| XbaI | TCTAGA | 1 cut(s) | 722 |
| XmiI | GTMKAC | 1 cut(s) | 2093 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2216 |
| XspI | CTAG | 7 cut(s) | 498, 723, 816, 1266, 1557, 1641, 2097 |
| ZrmI | AGTACT | 1 cut(s) | 1042 |
| Zsp2I | ATGCAT | 1 cut(s) | 508 |