FvH4_7g06520

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0020346
Physical Location & Seq
Reverse (-)
6534483 .. 6537759
3277 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06520.t1

Sequence Viewer

Length: 3090 bp
ATGAAGCTTGTCCCCATGGAATCTGGCCTTGTTTTATATTCTCTTCCCATCAAGGTTCTGTTATGTTGTTTGTGTTTCTTGCTAGCTGGTTCTTCCCTAGCCAGTAGTGCCACTAACCCCACAAGGTTGAGAGGGAAAGAGACTGACCGAGTTGCACTTCTAGCCATCAAGGCTCAAATAAAGCATGAACCTACCTCGGTCCTGAGCTCCTGGAATGAATCCAGTCACTTCTGCCAGTGGCAAGGAGTTGGTTGCGGGCGCCGGCATCAAAGGGTGACAGTGTTAGACCTACAGTCCCAGCATTTGGCAGGATCCATATCCCCCCACGTAGGGAATCTAAGCTTTCTCAAGGAGCTATATCTACAAAACAATAGCTTCACAGGTGAGATCCCACCACATATTGGTCACTTGAGGAGGTTGCAGACTCTAAGACTGAACAATAATTCCATCGGTGGTGAAATCCCTGCCAATATATCCAGTTGCTCCAACCTCATTCGACTAGATTTAGGGTACAACAAGCTGGTTGGGAAACTTCCTACCACAATTGGGTCCTTGTCAATGCTCCAGAGGTTTGGTTTTTACTATAACAATCTAGCAGGAACGATCCCACCTTCATTTGGCAACCTTTCATCCCTGACCACGCTTTTGGCTATTGGTAACAATTTGGAGGGAAGTATTCCTATTTCCATAGGGAAACTTACAAGCTTAAAAGTCTTTGCTGTGGGAGAAAATATGTTGTCAGGTGCTGTTCCTGCTTTCATTTTTAACATGTCTTCTCTCACTATGTTGGACTTGTTGGGAAACCAATTCCATGGCAGGCTTCCTTCAGACATGGGCAACACTCTTCCTAATCTCCAAGCCATAGATGCTTCTTTCAACCAAATCACAGGACCCATTCCCATGTCCATATCCAATGCCTCCAATCTTAGACAATTTATTGTTGCAGTAAATGGCTTAACTGGACAAGTTCCCTCTTTACAGAAGCTATATGGACTCTGGGAATTTGTAATTGGCGACAATTATATTGGAAGTGGGAAAGCCAGTGACTTTAGCTTTCTCTCGGATTTGACCAACTCCACACAACTGGGGACTCTTGATTTAACCGCCAACAATTTTGGAGGGATGCTTCCCCTGAGTTTATGGAACTTCTCAATAAGTCTGTCTGCGTTTTCTGTCCTGAATAATAAGATACATGGAAGCATCCCAAATGGGATAGGCAAACTTGTCAACATGGAGATTCTGTATATGGGCGACAATGAATTTAGTGGAAGCATTCCTGCTGATATTGGGAAGCTTCGGAGTTTAGGGAGATTGCTTCTTAACAACAACAAATTATCAGGGGATATCCCTTCCTCCCTTGGGAATTTAACAGAGTTATTCACACTTGACTTGCAAGGAAATGCTCTTCAGGGCATCATCCCTCCGAGTTTGGGGAGATGTCAGAAGCTGCTTCAGTTGCAACTTTCTCAAAACAATCTCAGTGGTGTATTAACTCCACAAGTTTGGAGTCTCTCATCATTAGCATTGGGTTTGAACCTATCTCACAACCACTTGACTGGTTCTCTTCCCATGGAAGTTGGAAATCTAAAAGGTCTGAGTTCTTTGGACCTTTCAGACAACATGTTATCCGGAGAAATTCCAACCAGTATTGGCAAGTGTCAAAGTTTAGAAGTCCTCCACTTGCAAGGCAACTCCTTCCAAGGGACCATTTCATCTTTGGAACCATTGAGAGGGCTTCAATTCTTGGATCTCTCTAGAAACAACCTCTCAGGGGAGATTCCACAATACTTGGAGAGGTTTCAATTGCTAAACTTGAATCTCTCTTTCAATGACTTCCAGGGTGAGCTGCCAGTTGGAGGGGTCTTCAAAAATGCCAGTGCAATTTCCATTGTTGGAAACAGCAAGCTATGCGGCGGTGTTGCTCAACTCCAGCTTCACAAATGCAACTACCCGTTCAAAGGTTCCAAGAAAAAGAGCTCCAAATTGGTAATGTTCATAGTCCCTGGACTTGTGGTGGTCATCTTGGTGGTGCTGTGTTTCCTATATTTTCTTCCATTAAGAAAAAAGCTGAAGAAAGAACCTGCATCAAGCAATTTGGAAAAACTGTCTCAAGTGTCATATGATGCTCTTCTAAAAGCTACTGATGGGTTCTCTTCAGCTAATTTGGTTGGTACTGGTAGTTTTGGGACGGTCTACAAAGGAGTGCTGAATGATGTTGGAACTCTTGTTGCTGTTAAAGTTTTCAACCTCTTACGCCAGGGAGCTTCTAGAAGTTTCATAGCCGAGTGTGAGGCATTGAGAAATATCAGGCATCGAAATCTGGTCAAGATTATTACTGCATGTTCAAGTATTGATTTTCTCGGTAATGATTTCAAGGCTCTGGTTTATGAGTTCATGGAGAATGGAAGCTTAGAGGAGTGGCTGCATCACACTACTGAAACTGAACAAGTAACAGGAGCACCCAAGAATTTGAACTTTCTTCAGAGGCTAGACATAGCCATTGATGTTGCTTCTGCACTAGATTATCTTCATAATCATTGTGAAACGCCAATAGTTCATTGTGATCTCAAACCAAGCAACGTCCTTTTAGATAAGAAATTCACTGGACATGTTTCTGATTTTGGACTAGCGAGGTTCCTAACCGATAACATTTCTGATAATCAAACACAATCCCTTGGACTAAGAGGGTCCATTGGCTATGCCGCACCAGAGTACGGAATGGGAAGTGAGGTGTCAACAGATGGGGATGTGTACAGCTTCGGCATCCTGTTGTTAGAGATGTTCACAGGAAAGAGACCTACTGACGACATGTTTAAAGACGGCTTGGACCTTCATGGTTTTGGTAAGATGGCTTGCTCTGACGGCGTTGCAAAGATTGCAGACTCGATGCTTCTTCAAGAAGACATTGTTGGTACAAGCATGATGAAAACTCCCAATCAGCAATGCGTGAGAGCTGGAAAAATTGAAGAGTGCTTGAGTCTGATCTTTGGAATTGGGATTGCTTGCTCTGCTGAATCACCAAGAGATCGAATGGATATTAGTGATGCTGCAGCTGGATTGCTTTCTGTAAGGAAAGTTCATGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1030

Amino Acids

111.2

Weight (kDa)

7.28

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 48 - 85 3.8e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 190 9.2e-12 Leucine-rich repeat region
LRR_14 PF23598 399 - 489 2.5e-06 Leucine-rich repeat region
LRR_14 PF23598 475 - 592 3.7e-07 Leucine-rich repeat region
Pkinase PF00069 719 - 935 3.7e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 721 - 937 1.4e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06520
rosa_chinensis RchiOBHm_Chr1g0334911
rosa_laevigata RLG00000029410
rosa_multiflora Rmu_sc0000574.1_g000054 Rmu_sc0015323.1_g000003
rosa_wichuraiana Rw1G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2089
AccB1I GGYRCC 1 cut(s) 258
AccB7I CCANNNNNTGG 2 cut(s) 304, 401
AccI GTMKAC 1 cut(s) 2193
AccIII TCCGGA 1 cut(s) 1628
AciI CCGC 5 cut(s) 255, 1104, 1911, 1914, 2703
AclWI GGATC 5 cut(s) 306, 319, 382, 598, 1755
AcsI RAATTY 6 cut(s) 1001, 1259, 1363, 1635, 2467, 2597
AcuI CTGAAG 6 cut(s) 810, 1391, 1436, 2090, 2139, 2465
AcyI GRCGYC 1 cut(s) 259
AfaI GTAC 5 cut(s) 512, 2173, 2714, 2753, 2914
AflIII ACRYGT 4 cut(s) 768, 1620, 2608, 2808
AhdI GACNNNNNGTC 1 cut(s) 292
AjnI CCWGG 4 cut(s) 209, 1836, 2002, 2256
AjuI GAANNNNNNNTTGG 4 cut(s) 993, 1025, 2892, 2924
AloI GAACNNNNNNTCC 2 cut(s) 428, 460
Alw21I GWGCWC 3 cut(s) 209, 1979, 2461
Alw26I GTCTC 4 cut(s) 134, 1514, 2112, 2788
AlwI GGATC 5 cut(s) 306, 319, 382, 598, 1755
AlwNI CAGNNNCTG 2 cut(s) 746, 1447
Aor13HI TCCGGA 1 cut(s) 1628
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 5 cut(s) 1447, 1846, 2422, 3047, 3050
ApoI RAATTY 6 cut(s) 1001, 1259, 1363, 1635, 2467, 2597
ArsI GACNNNNNNTTYG 2 cut(s) 628, 660
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
AsuHPI GGTGA 5 cut(s) 286, 395, 467, 1853, 3009
AsuNHI GCTAGC 1 cut(s) 82
AvaII GGWCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
BamHI GGATCC 1 cut(s) 311
BanI GGYRCC 1 cut(s) 258
BanII GRGCYC 2 cut(s) 209, 1979
BbsI GAAGAC 3 cut(s) 765, 1855, 2907
Bbv12I GWGCWC 3 cut(s) 209, 1979, 2461
BbvI GCAGC 5 cut(s) 1434, 1833, 2409, 3034, 3062
BccI CCATC 6 cut(s) 56, 173, 455, 2138, 2735, 2842
BceAI ACGGC 2 cut(s) 2836, 2878
BciT130I CCWGG 4 cut(s) 211, 1838, 2004, 2258
BcoDI GTCTC 4 cut(s) 134, 1514, 2112, 2788
BfmI CTRYAG 2 cut(s) 290, 3048
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 2089
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 7 cut(s) 1448, 1847, 1912, 2423, 2703, 3048, 3051
BlsI GCNGC 7 cut(s) 1449, 1848, 1913, 2424, 2704, 3049, 3052
Bme1390I CCNGG 4 cut(s) 211, 1838, 2004, 2258
Bme18I GGWCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgT120I GGNCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
BmiI GGNNCC 9 cut(s) 260, 313, 550, 892, 1705, 1722, 1963, 2636, 2689
BmrFI CCNGG 4 cut(s) 211, 1838, 2004, 2258
BmrI ACTGGG 1 cut(s) 1094
BmtI GCTAGC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 1094
BpiI GAAGAC 3 cut(s) 765, 1855, 2907
BpmI CTGGAG 2 cut(s) 548, 1913
Bpu10I CCTNAGC 1 cut(s) 203
BpuEI CTTGAG 4 cut(s) 332, 430, 2094, 2995
BsaAI YACGTR 1 cut(s) 328
BsaBI GATNNNNATC 1 cut(s) 316
BsaHI GRCGYC 1 cut(s) 259
BsaI GGTCTC 1 cut(s) 2788
BsaWI WCCGGW 1 cut(s) 1628
BsaXI ACNNNNNCTCC 2 cut(s) 1226, 1256
Bse118I RCCGGY 1 cut(s) 261
Bse3DI GCAATG 1 cut(s) 2948
Bse8I GATNNNNATC 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 1628
BseBI CCWGG 4 cut(s) 211, 1838, 2004, 2258
BseGI GGATG 6 cut(s) 629, 1128, 1200, 1416, 2752, 2763
BseJI GATNNNNATC 1 cut(s) 316
BseMI GCAATG 1 cut(s) 2948
BseMII CTCAG 5 cut(s) 194, 1124, 1492, 1586, 1782
BseRI GAGGAG 2 cut(s) 427, 2429
BseXI GCAGC 5 cut(s) 1434, 1833, 2409, 3034, 3062
BseYI CCCAGC 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 2499
BshFI GGCC 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 258
BsiHKAI GWGCWC 3 cut(s) 209, 1979, 2461
BsiSI CCGG 2 cut(s) 262, 1629
BslFI GGGAC 5 cut(s) 280, 1102, 1717, 1985, 2200
BsmAI GTCTC 4 cut(s) 134, 1514, 2112, 2788
BsmFI GGGAC 5 cut(s) 280, 1102, 1717, 1985, 2200
BsmI GAATGC 1 cut(s) 1272
BsnI GGCC 1 cut(s) 27
Bso31I GGTCTC 1 cut(s) 2788
Bsp1286I GDGCHC 3 cut(s) 209, 1979, 2461
Bsp13I TCCGGA 1 cut(s) 1628
Bsp1407I TGTACA 1 cut(s) 2751
Bsp143I GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
Bsp19I CCATGG 3 cut(s) 15, 811, 1569
BspACI CCGC 5 cut(s) 255, 1104, 1911, 1914, 2703
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 5 cut(s) 195, 1125, 1491, 1587, 1781
BspEI TCCGGA 1 cut(s) 1628
BspLI GGNNCC 9 cut(s) 260, 313, 550, 892, 1705, 1722, 1963, 2636, 2689
BspMAI CTGCAG 1 cut(s) 3052
BspMI ACCTGC 1 cut(s) 2089
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 5 cut(s) 306, 319, 382, 598, 1755
BspQI GCTCTTC 2 cut(s) 1410, 2133
BspT107I GGYRCC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 2788
BsrDI GCAATG 1 cut(s) 2948
BsrFI RCCGGY 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 2751
BssAI RCCGGY 1 cut(s) 261
BssMI GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
BssNI GRCGYC 1 cut(s) 259
BssT1I CCWWGG 6 cut(s) 15, 811, 1357, 1569, 1699, 2674
Bst2UI CCWGG 4 cut(s) 211, 1838, 2004, 2258
Bst4CI ACNGT 4 cut(s) 280, 294, 2106, 2191
Bst6I CTCTTC 7 cut(s) 48, 849, 1410, 1569, 2133, 2158, 2961
BstACI GRCGYC 1 cut(s) 259
BstAPI GCANNNNNTGC 2 cut(s) 1908, 2876
BstAUI TGTACA 1 cut(s) 2751
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 7 cut(s) 84, 257, 263, 818, 1904, 2854, 3004
BstDSI CCRYGG 3 cut(s) 15, 811, 1569
BstF5I GGATG 6 cut(s) 629, 1128, 1200, 1416, 2752, 2763
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 7 cut(s) 314, 390, 606, 1750, 2566, 2985, 3028
BstMAI GTCTC 4 cut(s) 134, 1514, 2112, 2788
BstMBI GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
BstNI CCWGG 4 cut(s) 211, 1838, 2004, 2258
BstNSI RCATGY 5 cut(s) 772, 1624, 2343, 2612, 2812
BstSCI CCNGG 4 cut(s) 209, 1836, 2002, 2256
BstSFI CTRYAG 2 cut(s) 290, 3048
BstV1I GCAGC 5 cut(s) 1434, 1833, 2409, 3034, 3062
BstV2I GAAGAC 3 cut(s) 765, 1855, 2907
BstX2I RGATCY 3 cut(s) 311, 387, 1747
BstXI CCANNNNNNTGG 6 cut(s) 572, 646, 812, 1084, 1503, 1556
BstYI RGATCY 3 cut(s) 311, 387, 1747
BsuRI GGCC 1 cut(s) 27
BtgI CCRYGG 3 cut(s) 15, 811, 1569
BtsCI GGATG 6 cut(s) 629, 1128, 1200, 1416, 2752, 2763
BtsIMutI CAGTG 6 cut(s) 242, 285, 1048, 1486, 1882, 2601
BveI ACCTGC 1 cut(s) 2089
Cac8I GCNNGC 7 cut(s) 84, 257, 263, 818, 1904, 2854, 3004
CaiI CAGNNNCTG 2 cut(s) 746, 1447
CfoI GCGC 1 cut(s) 261
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
Csp6I GTAC 5 cut(s) 511, 2172, 2713, 2752, 2913
CspCI CAANNNNNGTGG 4 cut(s) 1462, 1497, 1770, 1805
CviQI GTAC 5 cut(s) 511, 2172, 2713, 2752, 2913
DinI GGCGCC 1 cut(s) 260
DpnI GATC 7 cut(s) 313, 389, 605, 1749, 2565, 2984, 3027
DpnII GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
DraI TTTAAA 1 cut(s) 2815
DriI GACNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 7 cut(s) 48, 849, 1410, 1569, 2133, 2158, 2961
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 7 cut(s) 48, 849, 1410, 1569, 2133, 2158, 2961
Ecl136II GAGCTC 2 cut(s) 207, 1977
Eco130I CCWWGG 6 cut(s) 15, 811, 1357, 1569, 1699, 2674
Eco24I GRGCYC 2 cut(s) 209, 1979
Eco31I GGTCTC 1 cut(s) 2788
Eco32I GATATC 1 cut(s) 1345
Eco47I GGWCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
Eco53kI GAGCTC 2 cut(s) 207, 1977
Eco57I CTGAAG 6 cut(s) 810, 1391, 1436, 2090, 2139, 2465
EcoICRI GAGCTC 2 cut(s) 207, 1977
EcoO109I RGGNCCY 2 cut(s) 549, 890
EcoRII CCWGG 4 cut(s) 209, 1836, 2002, 2256
EcoRV GATATC 1 cut(s) 1345
EcoT14I CCWWGG 6 cut(s) 15, 811, 1357, 1569, 1699, 2674
EcoT38I GRGCYC 2 cut(s) 209, 1979
EgeI GGCGCC 1 cut(s) 260
EheI GGCGCC 1 cut(s) 260
ErhI CCWWGG 6 cut(s) 15, 811, 1357, 1569, 1699, 2674
FaqI GGGAC 5 cut(s) 280, 1102, 1717, 1985, 2200
FauI CCCGC 1 cut(s) 248
FauNDI CATATG 1 cut(s) 2119
FblI GTMKAC 1 cut(s) 2193
Fnu4HI GCNGC 7 cut(s) 1448, 1847, 1912, 2423, 2703, 3048, 3051
FokI GGATG 6 cut(s) 616, 1135, 1187, 1403, 2750, 2759
FriOI GRGCYC 2 cut(s) 209, 1979
Fsp4HI GCNGC 7 cut(s) 1448, 1847, 1912, 2423, 2703, 3048, 3051
GlaI GCGC 1 cut(s) 260
GluI GCNGC 7 cut(s) 1448, 1847, 1912, 2423, 2703, 3048, 3051
GsaI CCCAGC 1 cut(s) 301
GsuI CTGGAG 2 cut(s) 548, 1913
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 1 cut(s) 27
HapII CCGG 2 cut(s) 262, 1629
HhaI GCGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 259
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 2 cut(s) 1228, 2736
HindII GTYRAC 2 cut(s) 1228, 2736
HindIII AAGCTT 5 cut(s) 5, 340, 703, 1292, 2407
HpaII CCGG 2 cut(s) 262, 1629
HphI GGTGA 5 cut(s) 286, 395, 467, 1853, 3009
Hpy166II GTNNAC 5 cut(s) 1228, 2194, 2736, 2752, 2784
Hpy188III TCNNGA 9 cut(s) 202, 565, 1094, 1177, 1629, 1755, 2268, 2326, 2897
Hpy8I GTNNAC 5 cut(s) 1228, 2194, 2736, 2752, 2784
HpyAV CCTTC 5 cut(s) 621, 834, 1359, 1705, 2840
HpyCH4III ACNGT 4 cut(s) 280, 294, 2106, 2191
HpyCH4IV ACGT 2 cut(s) 327, 2580
HpySE526I ACGT 2 cut(s) 327, 2580
Hsp92I GRCGYC 1 cut(s) 259
HspAI GCGC 1 cut(s) 259
KasI GGCGCC 1 cut(s) 258
Kpn2I TCCGGA 1 cut(s) 1628
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
LguI GCTCTTC 2 cut(s) 1410, 2133
LmnI GCTCC 7 cut(s) 212, 352, 488, 567, 1982, 2261, 2456
Lsp1109I GCAGC 5 cut(s) 1434, 1833, 2409, 3034, 3062
MaeII ACGT 2 cut(s) 327, 2580
MaeIII GTNAC 6 cut(s) 224, 274, 404, 656, 1043, 2449
MalI GATC 7 cut(s) 313, 389, 605, 1749, 2565, 2984, 3027
MboI GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
MfeI CAATTG 2 cut(s) 543, 1802
MflI RGATCY 3 cut(s) 311, 387, 1747
MhlI GDGCHC 3 cut(s) 209, 1979, 2461
Mly113I GGCGCC 1 cut(s) 259
MlyI GAGTC 6 cut(s) 418, 987, 1084, 1516, 2876, 2986
MmeI TCCRAC 7 cut(s) 510, 768, 1558, 1664, 1834, 1873, 2197
MroI TCCGGA 1 cut(s) 1628
MroNI GCCGGC 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 899, 2024
MspA1I CMGCKG 1 cut(s) 3053
MspI CCGG 2 cut(s) 262, 1629
MspR9I CCNGG 4 cut(s) 211, 1838, 2004, 2258
MunI CAATTG 2 cut(s) 543, 1802
Mva1269I GAATGC 1 cut(s) 1272
MvaI CCWGG 4 cut(s) 211, 1838, 2004, 2258
NaeI GCCGGC 1 cut(s) 263
NarI GGCGCC 1 cut(s) 259
NcoI CCATGG 3 cut(s) 15, 811, 1569
NdeI CATATG 1 cut(s) 2119
NdeII GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
NgoMIV GCCGGC 1 cut(s) 261
NheI GCTAGC 1 cut(s) 82
NlaIV GGNNCC 9 cut(s) 260, 313, 550, 892, 1705, 1722, 1963, 2636, 2689
NmeAIII GCCGAG 1 cut(s) 2308
NmuCI GTSAC 4 cut(s) 224, 274, 404, 1043
NspI RCATGY 5 cut(s) 772, 1624, 2343, 2612, 2812
PciI ACATGT 4 cut(s) 768, 1620, 2608, 2808
PciSI GCTCTTC 2 cut(s) 1410, 2133
PctI GAATGC 1 cut(s) 1272
PdiI GCCGGC 1 cut(s) 263
PfeI GAWTC 7 cut(s) 20, 218, 334, 1237, 1777, 1816, 3014
PflMI CCANNNNNTGG 2 cut(s) 304, 401
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 7 cut(s) 1449, 1848, 1913, 2424, 2704, 3049, 3052
PleI GAGTC 6 cut(s) 418, 987, 1084, 1515, 2876, 2985
PluTI GGCGCC 1 cut(s) 262
PpsI GAGTC 6 cut(s) 418, 987, 1084, 1515, 2876, 2985
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 2 cut(s) 549, 890
PscI ACATGT 4 cut(s) 768, 1620, 2608, 2808
Psp124BI GAGCTC 2 cut(s) 209, 1979
Psp5II RGGWCCY 2 cut(s) 549, 890
Psp6I CCWGG 4 cut(s) 209, 1836, 2002, 2256
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 4 cut(s) 209, 1836, 2002, 2256
PspN4I GGNNCC 9 cut(s) 260, 313, 550, 892, 1705, 1722, 1963, 2636, 2689
PspPI GGNCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
PspPPI RGGWCCY 2 cut(s) 549, 890
PsrI GAACNNNNNNTAC 1 cut(s) 3060
PstI CTGCAG 1 cut(s) 3052
PstNI CAGNNNCTG 2 cut(s) 746, 1447
PsuI RGATCY 3 cut(s) 311, 387, 1747
PvuII CAGCTG 1 cut(s) 3053
RsaI GTAC 5 cut(s) 512, 2173, 2714, 2753, 2914
RsaNI GTAC 5 cut(s) 511, 2172, 2713, 2752, 2913
RseI CAYNNNNRTG 2 cut(s) 899, 2024
SacI GAGCTC 2 cut(s) 209, 1979
SapI GCTCTTC 2 cut(s) 1410, 2133
SatI GCNGC 7 cut(s) 1448, 1847, 1912, 2423, 2703, 3048, 3051
Sau3AI GATC 7 cut(s) 311, 387, 603, 1747, 2563, 2982, 3025
Sau96I GGNCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
SchI GAGTC 6 cut(s) 418, 987, 1084, 1516, 2876, 2986
ScrFI CCNGG 4 cut(s) 211, 1838, 2004, 2258
SduI GDGCHC 3 cut(s) 209, 1979, 2461
SfcI CTRYAG 2 cut(s) 290, 3048
SfoI GGCGCC 1 cut(s) 260
SinI GGWCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
SmiMI CAYNNNNRTG 2 cut(s) 899, 2024
SmlI CTYRAG 4 cut(s) 347, 409, 2109, 2974
SmoI CTYRAG 4 cut(s) 347, 409, 2109, 2974
SsiI CCGC 5 cut(s) 255, 1104, 1911, 1914, 2703
SspDI GGCGCC 1 cut(s) 258
SstI GAGCTC 2 cut(s) 209, 1979
StyD4I CCNGG 4 cut(s) 209, 1836, 2002, 2256
StyI CCWWGG 6 cut(s) 15, 811, 1357, 1569, 1699, 2674
TaaI ACNGT 4 cut(s) 280, 294, 2106, 2191
TaiI ACGT 2 cut(s) 330, 2583
TaqI TCGA 4 cut(s) 496, 2314, 2885, 3028
TaqII GACCGA 2 cut(s) 162, 187
TatI WGTACW 1 cut(s) 2751
TauI GCSGC 2 cut(s) 1914, 2705
TfiI GAWTC 7 cut(s) 20, 218, 334, 1237, 1777, 1816, 3014
TscAI CASTG 6 cut(s) 242, 285, 1048, 1486, 1882, 2608
TseFI GTSAC 4 cut(s) 224, 274, 404, 1043
TseI GCWGC 5 cut(s) 1447, 1846, 2422, 3047, 3050
Tsp45I GTSAC 4 cut(s) 224, 274, 404, 1043
TspGWI ACGGA 1 cut(s) 2730
TspRI CASTG 6 cut(s) 242, 285, 1048, 1486, 1882, 2608
Van91I CCANNNNNTGG 2 cut(s) 304, 401
VpaK11BI GGWCC 7 cut(s) 199, 549, 890, 1606, 1704, 2688, 2827
XapI RAATTY 6 cut(s) 1001, 1259, 1363, 1635, 2467, 2597
XbaI TCTAGA 2 cut(s) 1754, 2267
XceI RCATGY 5 cut(s) 772, 1624, 2343, 2612, 2812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1714
XmiI GTMKAC 1 cut(s) 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.