Rw1G011720

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0020346
Physical Location & Seq
Reverse (-)
26201552 .. 26204998
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011720.1

Sequence Viewer

Length: 3090 bp
ATGAAGCTTGTCCCCGTGAAATCATGCCTACTTTTCAAGGTAGTGTTATGTTGTTTGTGTTTCTTGCTATCAATTTTTTCCCTAGCCAGTGCCACCAACCCCACAAGGTTGAGAGGAAAAGAGACTGACCGAGTTGCACTTCTAGCCATCAAGGCTCAAATAAAGCATGAACATATCTCGGTCCTGAGCTCCTGGAATGAATCCAGTCACTTTTGCCAGTGGGAAGGAGTTGGTTGCAGTCGCCGGCATCAAAGGGTCATAGTGTTGGACCTACAGTCCCAGAAATTGGCAGGATCCATATCCCCCCACGTAGGTAATCTAAGCTTCCTCAAGGAGCTATATTTACAAAACAATAGCTTCACAGGTGAAATCCCACCACATATTGGTCACTTGAGAAGGCTGCAGATTTTAAGACTGGACAATAATTCCATCAGTGGCGAAATCCCTGCCAATATATCTAGCTGCTCCAACCTCATACGACTAGATGTCGGGTTCAACAGGCTGGTTGGGAAAATTCCTAGCACAATTGGGTCCTTGTCAAAGCTCAAGAACATTAGTTTTTACAATAACGGCCTAACAGGAACGATCCCACCTTCGTTTGGCAACCTTACATCCCTAACCAAGCTTTTGGCAATTGGTAATTTCTTGAAGGGAAGTATTCCAAGTTCTGTAGGGAAACTTACAAGCTTAGAAATCTTTGCTGTGGGACAGAATATGTTTTCTGGTGCTGTTCCTGCTGTGATTTTCAACATCTCATCTCTCACTGTGTTGGACTTGATGGGAAACCAATTCCATGGCAGGCTTCCCTCAGACATGGGCAACACTCTTCCTAAACTCCAAATTATAGATGTTGGCCTCAACGGATTCATCACCGGACCCATTCCCATGTCCATATCCAATGCCTCCAATCTTCGACAATTTATTGTTGCAGGAAACAGAATGACTGGACAAGTTCCCTCTTTGCAGAAGCTACACGGCCTCTGGGAATTTTTCATTTGGAGTAATCATCTTGGAAATGGGAAATCGGGTGACTTGAGCTTTCTCTCGGATTTGGCCAACTCCACGAAACTGGGGGGTGTTGAGATAAGCGGCAACAATTTTGGAGGGATGCTGCCCACATCTTTGTGGAACTTCTCAACAAGTCTTTACCTTTTTGGTGCGTCGACCAATCAGATACATGGAAGCATCCCAAATGGGATGGGCAATCTTGTCAACATGCAGTATCTGGATATGAGCAACAATGAATTCAATGGTAGCATTCCTGCCGATATTGGGAAGCTTCGGAATTTAGGGAGATTGAAGCTTTACAACAACAAACTATCAGGAGATATCCCCTCCTCCCTTGGGAACTTAACAGAGTTACTCGTACTTGACTTGGAAGGAAATGATCTTCAAGGCATCATCCCTCCAAGTTTGGGGAGATGCCAGTGGCTGCTTCAGTTGCAACTTTCTCAAAACAATCTCAGTGGTTTCTTAACTCAGCAAGTTTGGAGTCTCTCATCATTAGCAGTGGGTTTGAACCTGTCTCAAAACCACTTGACTGGTTCTCTCCCCAAGGAAGTTGGGAATCTGAAAGCTCTGGGTTCTTTGGACCTTTCTGATAACATGTTATCCGGGGAAATTCCAACCAGTATTGGCAAGTGTCAAAGTTTAGAGGTCCTCCACTTGCAAGGCAACTCCTTCCAAGGGACCATTACTTCATCTTTGGAAACATTGAGAGGGCTTCAATTCTTGGATCTCTCTCGAAACAACCTCTCAGGCAAGATTCCACGATACTTGGAGCGGTTTCAATTGCTAAACTTGAATCTCTCTTTCAATGACTTCGAGGGTGAGCTGCCAGTTGGAGGGGTCTTCAAAAATGCGAGTGCAATTTCCGTAGTTGGAAACAGCAAGCTCTGCGGTGGTGTTGCTCAACTCCAGCTGCCCAAATGCAACTACCCTTCCAAAGATTCCAAGAAAAGGAGCTTCAAATTGGTAATCTTCATAGTCCCTGGACTTGTGGTGGTAATCTTGGTGGTGCTGGGTTTCCTATATTTGATTCCATTAAGAAGGAAGCTAAGGAAAGAACCTGCATCAAGCAATTTGGAGAAACTGTTGCAAGTGTCATATAATGCTCTTTTAAAAGCTACTGATGGGTTCTCTTCAGCTAATTTGGTTGGTACTGGTAGTTTTGGGACGGTCTACAAAGGATTTCTGAATGATGTTGGTACTTTTGTTGCTGTCAAAGTGTTTAACCTGTTACGCCAGGGAGCTTCTAAGAGTTTCATAGCCGAGTGTGAGGCATTGAGAAATATCAGGCATCGAAATCTCGTCAAGATTATAACTGCATGTTCAAGTACTGATTTTCTTGGTAATGATTTCAAGGCTCTGGTTTATGAGTTCATGGAGAATGGAAGCTTAGACGAGTGGCTGCATCAAACTACTGAAACTGAACAAGTGACAGAAGCACCTAAGCATTTGAACTTTCTTCAGAGGCTAGACATAGCCATTGATGTTGCTAGCGCACTAGATTATCTTCATAACCATTGTGAAACGCCAATAGTTCATTGTGATCTCAAGCCAAGCAATGTCCTTTTAGATAATAAGGAACTGACTGGACGTGTTTCTGATTTTGGACTAGCAAGGTTTCTCTCTGAAGTTACAGATGACGTCTCTGCAAATCAAACACATTCACTGGGACTAAGAGGATCCATTGGCTATGCTGCACCAGAGTATGGAATGGGAAGTGAAGTGTCTACAGATGGGGATGTGTATAGCTTCGGCATCCTCTTGTTAGAGATGTTCACAGGAAAGAGACCCACTGATGACATGTTTAAGGACGGCTTGAACCTTCATGGTTTTTGTAAGATGGCTTGCTCTGAGGGCGTTGCCAAGATTGCAGATTCAAGGCTTCTTCAAGAAGACATGGTTGCTACAGGCATCAACAAAACTCCCAATCAGCGCTGCGTGAGAGCTGAAAAACTTGAAGAGTGCTTGAGTCTGATCTTTGGAATTGGGATTGCTTGTTCTGCTGAATCACCGAGAGACCGACTGGACGTTAGAGATGCTGCATCTGAATTGCATTCCATCAGGAACATTCTTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1029

Amino Acids

112.02

Weight (kDa)

8.36

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 80 1.7e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 85 - 209 1e-12 Leucine-rich repeat region
LRR_14 PF23598 390 - 483 6.3e-08 Leucine-rich repeat region
LRR_14 PF23598 470 - 580 4e-08 Leucine-rich repeat region
Pkinase PF00069 715 - 941 7.6e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 717 - 938 2.9e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06520
rosa_chinensis RchiOBHm_Chr1g0334911
rosa_laevigata RLG00000029410
rosa_multiflora Rmu_sc0000574.1_g000054 Rmu_sc0015323.1_g000003
rosa_wichuraiana Rw1G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2321
AatII GACGTC 1 cut(s) 2652
Acc36I ACCTGC 1 cut(s) 2077
AccB7I CCANNNNNTGG 3 cut(s) 286, 383, 2714
AccBSI CCGCTC 1 cut(s) 1783
AccI GTMKAC 3 cut(s) 1163, 2181, 2735
AciI CCGC 3 cut(s) 1089, 1783, 1899
AclWI GGATC 6 cut(s) 288, 301, 580, 1743, 2682, 2695
AcoI YGGCCR 1 cut(s) 1053
AcsI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
AcuI CTGAAG 4 cut(s) 1421, 2127, 2453, 2655
AcyI GRCGYC 1 cut(s) 2649
AfaI GTAC 4 cut(s) 1368, 2161, 2209, 2338
AfeI AGCGCT 1 cut(s) 2942
AflIII ACRYGT 3 cut(s) 1605, 2599, 2808
AhdI GACNNNNNGTC 2 cut(s) 274, 485
AjiI CACGTC 1 cut(s) 2600
AjnI CCWGG 3 cut(s) 191, 1990, 2244
AloI GAACNNNNNNTCC 2 cut(s) 2989, 3021
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 6 cut(s) 116, 1499, 1530, 2656, 2788, 3018
AlwI GGATC 6 cut(s) 288, 301, 580, 1743, 2682, 2695
AlwNI CAGNNNCTG 2 cut(s) 1225, 1432
Aor51HI AGCGCT 1 cut(s) 2942
AoxI GGCC 4 cut(s) 571, 853, 976, 1053
ApoI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
AspLEI GCGC 2 cut(s) 2504, 2943
AspS9I GGNCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
AsuC2I CCSGG 1 cut(s) 1615
AsuHPI GGTGA 5 cut(s) 377, 862, 1040, 1841, 3009
AsuNHI GCTAGC 1 cut(s) 2498
AvaII GGWCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
BaeI ACNNNNGTAYC 2 cut(s) 2199, 2232
BalI TGGCCA 1 cut(s) 1055
BamHI GGATCC 2 cut(s) 293, 2687
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 1843, 2907
Bbv12I GWGCWC 1 cut(s) 191
BccI CCATC 8 cut(s) 155, 437, 772, 1192, 2126, 2735, 2842, 3074
BceAI ACGGC 3 cut(s) 586, 991, 2836
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 3 cut(s) 193, 1992, 2246
BcnI CCSGG 1 cut(s) 1615
BcoDI GTCTC 6 cut(s) 116, 1499, 1530, 2656, 2788, 3018
BfaI CTAG 9 cut(s) 83, 143, 459, 482, 519, 2477, 2499, 2507, 2618
BfmI CTRYAG 5 cut(s) 272, 401, 669, 2736, 2913
BfoI RGCGCY 1 cut(s) 2944
BfuAI ACCTGC 1 cut(s) 2077
BglI GCCNNNNNGGC 1 cut(s) 152
BmcAI AGTACT 1 cut(s) 2338
Bme1390I CCNGG 4 cut(s) 193, 1615, 1992, 2246
Bme18I GGWCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
BmeRI GACNNNNNGTC 2 cut(s) 274, 485
BmgBI CACGTC 1 cut(s) 2600
BmgT120I GGNCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
BmiI GGNNCC 5 cut(s) 295, 532, 877, 1690, 2689
BmrFI CCNGG 4 cut(s) 193, 1615, 1992, 2246
BmrI ACTGGG 2 cut(s) 1079, 2684
BmtI GCTAGC 1 cut(s) 2502
BmuI ACTGGG 2 cut(s) 1079, 2684
BpiI GAAGAC 2 cut(s) 1843, 2907
BpmI CTGGAG 1 cut(s) 1901
Bpu10I CCTNAGC 3 cut(s) 185, 2057, 2451
BpuEI CTTGAG 6 cut(s) 314, 412, 530, 1054, 2540, 2995
BpuMI CCSGG 1 cut(s) 1615
BsaAI YACGTR 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 2649
BsaI GGTCTC 2 cut(s) 2788, 3018
BsaJI CCNNGG 7 cut(s) 793, 1342, 1554, 1614, 1684, 1990, 2245
BsaWI WCCGGW 1 cut(s) 872
BsaXI ACNNNNNCTCC 2 cut(s) 2078, 2108
Bse118I RCCGGY 1 cut(s) 243
Bse3DI GCAATG 1 cut(s) 2572
Bse8I GATNNNNATC 1 cut(s) 298
BseBI CCWGG 3 cut(s) 193, 1992, 2246
BseDI CCNNGG 7 cut(s) 793, 1342, 1554, 1614, 1684, 1990, 2245
BseGI GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2752, 2763
BseJI GATNNNNATC 1 cut(s) 298
BseMI GCAATG 1 cut(s) 2572
BseMII CTCAG 6 cut(s) 176, 822, 1477, 1493, 1770, 2850
BseRI GAGGAG 1 cut(s) 1327
BseYI CCCAGC 1 cut(s) 2020
BsgI GTGCAG 1 cut(s) 2688
BshFI GGCC 4 cut(s) 573, 855, 978, 1055
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 3 cut(s) 244, 873, 1614
BslFI GGGAC 6 cut(s) 262, 720, 1702, 1973, 2188, 2691
BsmAI GTCTC 6 cut(s) 116, 1499, 1530, 2656, 2788, 3018
BsmBI CGTCTC 1 cut(s) 2656
BsmFI GGGAC 6 cut(s) 262, 720, 1702, 1973, 2188, 2691
BsmI GAATGC 2 cut(s) 1257, 3061
BsnI GGCC 4 cut(s) 573, 855, 978, 1055
Bso31I GGTCTC 2 cut(s) 2788, 3018
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
Bsp19I CCATGG 1 cut(s) 793
BspACI CCGC 3 cut(s) 1089, 1783, 1899
BspANI GGCC 4 cut(s) 573, 855, 978, 1055
BspCNI CTCAG 6 cut(s) 177, 821, 1476, 1492, 1769, 2851
BspLI GGNNCC 5 cut(s) 295, 532, 877, 1690, 2689
BspMAI CTGCAG 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 2077
BspOI GCTAGC 1 cut(s) 2502
BspPI GGATC 6 cut(s) 288, 301, 580, 1743, 2682, 2695
BspTNI GGTCTC 2 cut(s) 2788, 3018
BsrBI CCGCTC 1 cut(s) 1783
BsrDI GCAATG 1 cut(s) 2572
BsrFI RCCGGY 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 7 cut(s) 793, 1342, 1554, 1614, 1684, 1990, 2245
BssMI GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
BssNI GRCGYC 1 cut(s) 2649
BssT1I CCWWGG 4 cut(s) 793, 1342, 1554, 1684
Bst2UI CCWGG 3 cut(s) 193, 1992, 2246
Bst4CI ACNGT 4 cut(s) 276, 766, 2094, 2179
Bst6I CTCTTC 3 cut(s) 831, 2146, 2961
BstACI GRCGYC 1 cut(s) 2649
BstAPI GCANNNNNTGC 1 cut(s) 1896
BstBAI YACGTR 1 cut(s) 310
BstC8I GCNNGC 5 cut(s) 245, 800, 1892, 2500, 2854
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2752, 2763
BstH2I RGCGCY 1 cut(s) 2944
BstHHI GCGC 2 cut(s) 2504, 2943
BstKTI GATC 7 cut(s) 296, 588, 1390, 1738, 2554, 2690, 2985
BstMAI GTCTC 6 cut(s) 116, 1499, 1530, 2656, 2788, 3018
BstMBI GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
BstMWI GCNNNNNNNGC 8 cut(s) 143, 152, 734, 1441, 1896, 2862, 2876, 3008
BstNI CCWGG 3 cut(s) 193, 1992, 2246
BstNSI RCATGY 4 cut(s) 1219, 1609, 2331, 2812
BstSCI CCNGG 4 cut(s) 191, 1613, 1990, 2244
BstSFI CTRYAG 5 cut(s) 272, 401, 669, 2736, 2913
BstV2I GAAGAC 2 cut(s) 1843, 2907
BstX2I RGATCY 3 cut(s) 293, 1735, 2687
BstXI CCANNNNNNTGG 4 cut(s) 628, 794, 1069, 1541
BstYI RGATCY 3 cut(s) 293, 1735, 2687
BsuRI GGCC 4 cut(s) 573, 855, 978, 1055
BtgI CCRYGG 1 cut(s) 793
BtrI CACGTC 1 cut(s) 2600
BtsCI GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2752, 2763
BtsI GCAGTG 1 cut(s) 1515
BtsIMutI CAGTG 9 cut(s) 94, 224, 439, 762, 1433, 1471, 1515, 2672, 2799
BveI ACCTGC 1 cut(s) 2077
Cac8I GCNNGC 5 cut(s) 245, 800, 1892, 2500, 2854
CaiI CAGNNNCTG 2 cut(s) 1225, 1432
CfoI GCGC 2 cut(s) 2504, 2943
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
CseI GACGC 1 cut(s) 1149
Csp6I GTAC 4 cut(s) 1367, 2160, 2208, 2337
CspCI CAANNNNNGTGG 4 cut(s) 1447, 1482, 1758, 1793
CviQI GTAC 4 cut(s) 1367, 2160, 2208, 2337
DpnI GATC 7 cut(s) 295, 587, 1389, 1737, 2553, 2689, 2984
DpnII GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
DraI TTTAAA 1 cut(s) 2121
DriI GACNNNNNGTC 2 cut(s) 274, 485
EaeI YGGCCR 1 cut(s) 1053
Eam1104I CTCTTC 3 cut(s) 831, 2146, 2961
Eam1105I GACNNNNNGTC 2 cut(s) 274, 485
EarI CTCTTC 3 cut(s) 831, 2146, 2961
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 4 cut(s) 793, 1342, 1554, 1684
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 2 cut(s) 2788, 3018
Eco32I GATATC 1 cut(s) 1330
Eco47I GGWCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
Eco47III AGCGCT 1 cut(s) 2942
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 4 cut(s) 1421, 2127, 2453, 2655
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 2 cut(s) 531, 1657
EcoRI GAATTC 1 cut(s) 1244
EcoRII CCWGG 3 cut(s) 191, 1990, 2244
EcoRV GATATC 1 cut(s) 1330
EcoT14I CCWWGG 4 cut(s) 793, 1342, 1554, 1684
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 4 cut(s) 793, 1342, 1554, 1684
Esp3I CGTCTC 1 cut(s) 2656
FalI AAGNNNNNCTT 2 cut(s) 2807, 2839
FaqI GGGAC 6 cut(s) 262, 720, 1702, 1973, 2188, 2691
FblI GTMKAC 3 cut(s) 1163, 2181, 2735
FokI GGATG 7 cut(s) 598, 1120, 1172, 1210, 1388, 2750, 2759
FriOI GRGCYC 1 cut(s) 191
FspBI CTAG 9 cut(s) 83, 143, 459, 482, 519, 2477, 2499, 2507, 2618
GlaI GCGC 2 cut(s) 2503, 2942
GsaI CCCAGC 1 cut(s) 2024
GsuI CTGGAG 1 cut(s) 1901
HaeII RGCGCY 1 cut(s) 2944
HaeIII GGCC 4 cut(s) 573, 855, 978, 1055
HapII CCGG 3 cut(s) 244, 873, 1614
HgaI GACGC 1 cut(s) 1149
HhaI GCGC 2 cut(s) 2504, 2943
Hin1I GRCGYC 1 cut(s) 2649
Hin6I GCGC 2 cut(s) 2502, 2941
HinP1I GCGC 2 cut(s) 2502, 2941
HincII GTYRAC 2 cut(s) 1164, 1213
HindII GTYRAC 2 cut(s) 1164, 1213
HindIII AAGCTT 7 cut(s) 5, 322, 623, 685, 1277, 1301, 2395
HpaII CCGG 3 cut(s) 244, 873, 1614
HphI GGTGA 5 cut(s) 377, 862, 1040, 1841, 3009
Hpy166II GTNNAC 5 cut(s) 1164, 1213, 2182, 2736, 2784
Hpy188III TCNNGA 9 cut(s) 184, 547, 646, 1226, 1323, 1743, 2314, 2897, 3070
Hpy8I GTNNAC 5 cut(s) 1164, 1213, 2182, 2736, 2784
Hpy99I CGWCG 1 cut(s) 1165
HpyAV CCTTC 9 cut(s) 218, 390, 603, 643, 1373, 1690, 1950, 2043, 2840
HpyCH4III ACNGT 4 cut(s) 276, 766, 2094, 2179
HpyCH4IV ACGT 4 cut(s) 309, 2599, 2649, 3036
HpyF10VI GCNNNNNNNGC 8 cut(s) 143, 152, 734, 1441, 1896, 2862, 2876, 3008
HpySE526I ACGT 4 cut(s) 309, 2599, 2649, 3036
Hsp92I GRCGYC 1 cut(s) 2649
HspAI GCGC 2 cut(s) 2502, 2941
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Kzo9I GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
LmnI GCTCC 6 cut(s) 194, 334, 470, 1780, 1962, 2249
MaeI CTAG 9 cut(s) 83, 143, 459, 482, 519, 2477, 2499, 2507, 2618
MaeII ACGT 4 cut(s) 309, 2599, 2649, 3036
MaeIII GTNAC 7 cut(s) 206, 386, 1028, 1359, 2238, 2437, 2638
MalI GATC 7 cut(s) 295, 587, 1389, 1737, 2553, 2689, 2984
MbiI CCGCTC 1 cut(s) 1783
MboI GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
MfeI CAATTG 3 cut(s) 525, 633, 1790
MflI RGATCY 3 cut(s) 293, 1735, 2687
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 1055
MluNI TGGCCA 1 cut(s) 1055
MlyI GAGTC 2 cut(s) 1501, 2986
MmeI TCCRAC 6 cut(s) 246, 492, 750, 1649, 1822, 1861
Mox20I TGGCCA 1 cut(s) 1055
MroNI GCCGGC 1 cut(s) 243
MscI TGGCCA 1 cut(s) 1055
MseI TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2814
MslI CAYNNNNRTG 2 cut(s) 884, 1123
Msp20I TGGCCA 1 cut(s) 1055
MspA1I CMGCKG 1 cut(s) 1921
MspI CCGG 3 cut(s) 244, 873, 1614
MspR9I CCNGG 4 cut(s) 193, 1615, 1992, 2246
MunI CAATTG 3 cut(s) 525, 633, 1790
Mva1269I GAATGC 2 cut(s) 1257, 3061
MvaI CCWGG 3 cut(s) 193, 1992, 2246
MwoI GCNNNNNNNGC 8 cut(s) 143, 152, 734, 1441, 1896, 2862, 2876, 3008
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 1615
NcoI CCATGG 1 cut(s) 793
NdeII GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
NgoMIV GCCGGC 1 cut(s) 243
NheI GCTAGC 1 cut(s) 2498
NlaIV GGNNCC 5 cut(s) 295, 532, 877, 1690, 2689
NmeAIII GCCGAG 1 cut(s) 2296
NmuCI GTSAC 4 cut(s) 206, 386, 1028, 2437
NspI RCATGY 4 cut(s) 1219, 1609, 2331, 2812
PciI ACATGT 2 cut(s) 1605, 2808
PctI GAATGC 2 cut(s) 1257, 3061
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 9 cut(s) 200, 864, 1567, 1765, 1804, 1949, 2038, 2882, 3014
PflMI CCANNNNNTGG 3 cut(s) 286, 383, 2714
PfoI TCCNGGA 1 cut(s) 191
PleI GAGTC 2 cut(s) 1500, 2985
PpsI GAGTC 2 cut(s) 1500, 2985
Ppu21I YACGTR 1 cut(s) 310
PpuMI RGGWCCY 2 cut(s) 531, 1657
PscI ACATGT 2 cut(s) 1605, 2808
PsiI TTATAA 1 cut(s) 2321
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 2 cut(s) 531, 1657
Psp6I CCWGG 3 cut(s) 191, 1990, 2244
PspFI CCCAGC 1 cut(s) 2020
PspGI CCWGG 3 cut(s) 191, 1990, 2244
PspN4I GGNNCC 5 cut(s) 295, 532, 877, 1690, 2689
PspPI GGNCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
PspPPI RGGWCCY 2 cut(s) 531, 1657
PsrI GAACNNNNNNTAC 2 cut(s) 649, 681
PstI CTGCAG 1 cut(s) 405
PstNI CAGNNNCTG 2 cut(s) 1225, 1432
PsuI RGATCY 3 cut(s) 293, 1735, 2687
PvuII CAGCTG 1 cut(s) 1921
RsaI GTAC 4 cut(s) 1368, 2161, 2209, 2338
RsaNI GTAC 4 cut(s) 1367, 2160, 2208, 2337
RseI CAYNNNNRTG 2 cut(s) 884, 1123
SacI GAGCTC 1 cut(s) 191
SalI GTCGAC 1 cut(s) 1162
SaqAI TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2814
Sau3AI GATC 7 cut(s) 293, 585, 1387, 1735, 2551, 2687, 2982
Sau96I GGNCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
ScaI AGTACT 1 cut(s) 2338
SchI GAGTC 2 cut(s) 1501, 2986
ScrFI CCNGG 4 cut(s) 193, 1615, 1992, 2246
SduI GDGCHC 1 cut(s) 191
SfcI CTRYAG 5 cut(s) 272, 401, 669, 2736, 2913
SinI GGWCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
SmiMI CAYNNNNRTG 2 cut(s) 884, 1123
SmlI CTYRAG 6 cut(s) 329, 391, 545, 1033, 2555, 2974
SmoI CTYRAG 6 cut(s) 329, 391, 545, 1033, 2555, 2974
SsiI CCGC 3 cut(s) 1089, 1783, 1899
SspMI CTAG 9 cut(s) 83, 143, 459, 482, 519, 2477, 2499, 2507, 2618
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 191, 1613, 1990, 2244
StyI CCWWGG 4 cut(s) 793, 1342, 1554, 1684
TaaI ACNGT 4 cut(s) 276, 766, 2094, 2179
TaiI ACGT 4 cut(s) 312, 2602, 2652, 3039
TaqI TCGA 5 cut(s) 913, 1163, 1744, 1824, 2302
TaqII GACCGA 3 cut(s) 144, 169, 3042
TatI WGTACW 1 cut(s) 2336
TauI GCSGC 1 cut(s) 1092
TfiI GAWTC 9 cut(s) 200, 864, 1567, 1765, 1804, 1949, 2038, 2882, 3014
Tru1I TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2814
Tru9I TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2814
TscAI CASTG 9 cut(s) 94, 224, 439, 769, 1433, 1471, 1515, 2679, 2806
TseFI GTSAC 4 cut(s) 206, 386, 1028, 2437
Tsp45I GTSAC 4 cut(s) 206, 386, 1028, 2437
TspGWI ACGGA 2 cut(s) 876, 1864
TspRI CASTG 9 cut(s) 94, 224, 439, 769, 1433, 1471, 1515, 2679, 2806
Van91I CCANNNNNTGG 3 cut(s) 286, 383, 2714
VpaK11BI GGWCC 7 cut(s) 181, 268, 531, 875, 1591, 1657, 1689
XapI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
XceI RCATGY 4 cut(s) 1219, 1609, 2331, 2812
XmiI GTMKAC 3 cut(s) 1163, 2181, 2735
XspI CTAG 9 cut(s) 83, 143, 459, 482, 519, 2477, 2499, 2507, 2618
ZraI GACGTC 1 cut(s) 2650
ZrmI AGTACT 1 cut(s) 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.