RchiOBHm_Chr1g0334911

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0020346
Physical Location & Seq
Reverse (-)
27128544 .. 27131825
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56359

Sequence Viewer

Length: 3108 bp
ATGAAGCTTGTCCCCGTAAAATCATGCCTACTTTTCAAGGTAGTGTTATGTTGTTTTTGTTTCTTGCTATCAATTTTTTCCCTAGCCAGTGCCACCAACCCCACAAGGTTGAGAGGAAAAGAGACTGATCGAGTTGCACTTCTAGCCATCAAGGCTCAACTAAAGCATGAACATATCTCGGTCCTGAGCTCCTGGAATGAATCCAGTCACTTTTGCCAGTGGGAAGGAGTTGGTTGCAGTCGCCGACATCAAAGGGTCACAGTGTTGGACCTACAGTCCCAGAAATTGGCAGGATCCATATCCCCCCACGTAGGTAATCTAAGCTTCCTCAAGGAGCTATATTTACAAAACAATAGCTTCACAGGTGAAATCCCACCACATATTGGTCACTTGAGGAGGCTGCAGATTTTAAGACTGGACAATAATTCCATCAGTGGCGAAATCCCTGCCAATATATCTAGCTGCTCCAACCTCATACGACTAGATGTCGGGTTCAACAGGCTGGTTGGGAAAATTCCTAGCACAATTGGGTCCTTGTCAAAGCTCAAGAACATTAGTTTTTACAATAACGGCCTAACAGGAACGATCCCACCTTCATTTGGCAACCTTACATCCCTAACCAAGCTTTTGGCAATTGGTAATTTCTTGAAGGGAAGTATTCCAAGTTCTGTAGGGAAACTTACAAGCTTAGAAATCTTTGCTGTGGGACAGAATATGTTTTCTGGTGCTGTTCCTGCTGTGATTTTCAACATCTCATCTCTCACTGTGTTGGACTTGATGGGAAACCAATTCCATGGCAGGCTTCCCTCAGACATGGGCAACACTCTTCCTAATCTCCAAATTATAGATGTTGGCCTCAACGGATTCATCACCGGACCCATTCCCATGTCCATATCCAATGCCTCCAATCTTAGACAATTTATTGTTGCAGGAAACAGAATAACTGGACAAGTTCCCTCTTTGCAGAAGCTACACGGCCTCTGGGAATTTTTCATTTGGAGTAATCATCTTGGAAATGGGAAATCGGGTGACTTGAGCTTTCTCTCGGATTTGGCCAACTCCACGAAACTGGGGGGTGTTGAGATAAGTGGCAACAATTTTGGAGGGATGCTGCCCACATCTTTATGGAACTTCTCAACAAGTCTTTACCTTTTTGGTGCGTCGACCAATCAGATACATGGAAGCATCCCAAATGGGATGGGCAATCTTGTCAACATGCAGTATCTGGATATGAGCAACAATGAATTCAATGGTAGCATTCCTGCCGATATTGGGAAGCTTCGAAATTTAGGGAGATTGAAGCTTTACAACAACAAACTATCAGGAGATATCCCCTCCTCCCTTGGGAACTTAACAGAGTTACTCGTACTTGACTTGGAAGGAAATGATCTTCAAGGCATCATCCCTCCAAGTTTGGGGAGATGCCAGTGGCTACTTCAGTTGCAACTTTCTCAAAACAATCTCAGTGGTTTCTTAACTCAGCAAGTTTGGAGTCTCTCATCATTAGCAGTGGGTTTGAACCTATCTCAAAACCACTTGACTGGTTCTCTCCCTAAGGAAGTTGGGAATCTGAAAGCTCTGGGTTCTTTGGACCTTTCTGATAACATGTTATCCGGGGAAATTCCAACCAGTATTGGCAAGTGTCAAAGTTTAGAAGTCCTCCACTTGCAAGGGAACTCCCTCCAAGGGACCATTTCTTCATCTTTGGAAACGTTGAGAGGGCTTCAATTCTTGGATCTCTCTCGAAACAACCTCTCAGGCAAGATTCCACTATATTTGGAGGGGTTTCAATTGCTAAACTTGAATCTCTCTTTCAATGACTTCGAGGGTGAGCTGCCAGTTGGAGGGGTCTTCAAAAATGCGAGTGCAATTTCCATTGTTGGAAACAGCAAGCTCTGCGGCGGTGTTGCTCAACTCCAGCTACCCAAATGCAACTACCCTTCCAAAGATTCCAAGAAAAGGAGCTTCAAATTGGTAATCTTCATAGTCCTTGGACTTGTGGTGGTAATCTTGGTGGTGCTGGGTTTCCTATATTTGATTCCATTAAGAAAGAAGCTAAGGAAACAACCTGCATCAAGCAATTTGGAGAAACTGTTGCAAGTGTCATATAATTCTCTTTTAAAAGCTACTGATGGGTTCTCTTCAGCTAATTTGGTTGGTACTGGTAGTTTTGGGACGGTCTACAAAGGATTTCTGAATGATGTTGGTACTCTTGTTGCTGTCAAAGTTTTTAACCTGTTACGCCAGGGAGTTTCTAAGAGTTTCATAGCCGAGTGTGAGGTATTGAGAAATATCAGGCATCGAAATCTCGTCAAGATTATAACTGCATGTTCAAGTACTGATTTTCTTGGTAATGATTTCAAGGCTCTGGTTTATGAGTTCATGGAGAATGGAAGCTTAGACGAGTGGCTGCATCAAACTACTGAAACTGAACAAGTGACAGAAGCACCCAAGCATTTGAACTTTCTTCAGAGGCTAGACATAGCCATTGATGTTGCTAGCGCACTAGATTATCTTCATAATCATTGTGAAACGCCAATAGTTCATTGTGATCTCAAGCCAAGCAATGTCCTTTTAGATAAGGAACTGATTGGACATGTTTCTGACTTTGGACTAGCACGGTTTGACTTTGGACTAGCAAGGTTTCTCTCTGAAGTTACAGATTACGTCTCTGCAAATCAAACACATTCACTGGGACTAAGAGGATCCATTGGCTATGCTGCACCAGAGTATGGAATGGGAAGTGAAGTGTCTACATATGGGGATGTGTATAGCTTCGGCATCCTCTTGTTAGAGATGTTCACAGGAAAGAGACCCATTGACGACATGTTTAAGGACGGCTTGAACCTTCATGGTTTTTGTAAGATGGCTTGCTCTGAGGGCGTTGCCAAGATTGCAGATTCAAGGCTTCTTCAAGAAGACATGGTTGCTACAGGCATCAACAAAACTCCCAATCAGCGCTGCGTGAGAGCTGAAAAACTTGAAGAGTGCTTGAGTCTGATCTTTGGAATTGGGATTGCTTGTTCTGCTGAATCACTGAGAGACCGAATGGGCATTAGAGATGCTGCATCAGAATTGCATTCCATCAGGAACATTCTTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1035

Amino Acids

112.65

Weight (kDa)

8.35

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 80 9.7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 84 - 188 9.2e-12 Leucine-rich repeat region
LRR_14 PF23598 390 - 480 9.7e-08 Leucine-rich repeat region
LRR_14 PF23598 470 - 580 7.1e-08 Leucine-rich repeat region
Pkinase PF00069 715 - 947 5.1e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 717 - 944 2.6e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06520
rosa_chinensis RchiOBHm_Chr1g0334911
rosa_laevigata RLG00000029410
rosa_multiflora Rmu_sc0000574.1_g000054 Rmu_sc0015323.1_g000003
rosa_wichuraiana Rw1G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2321
Acc36I ACCTGC 1 cut(s) 2077
AccB7I CCANNNNNTGG 3 cut(s) 286, 383, 2732
AccI GTMKAC 3 cut(s) 1163, 2181, 2753
AciI CCGC 2 cut(s) 1899, 1902
AclI AACGTT 1 cut(s) 1712
AclWI GGATC 6 cut(s) 288, 301, 580, 1743, 2700, 2713
AcoI YGGCCR 1 cut(s) 1053
AcsI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
AcuI CTGAAG 4 cut(s) 1421, 2127, 2453, 2673
AfaI GTAC 4 cut(s) 1368, 2161, 2209, 2338
AfeI AGCGCT 1 cut(s) 2960
AflIII ACRYGT 3 cut(s) 1605, 2596, 2826
AhdI GACNNNNNGTC 2 cut(s) 274, 485
AjnI CCWGG 2 cut(s) 191, 2244
AloI GAACNNNNNNTCC 2 cut(s) 3007, 3039
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 5 cut(s) 116, 1499, 2674, 2806, 3036
AlwI GGATC 6 cut(s) 288, 301, 580, 1743, 2700, 2713
AlwNI CAGNNNCTG 1 cut(s) 1225
Aor51HI AGCGCT 1 cut(s) 2960
AoxI GGCC 4 cut(s) 571, 853, 976, 1053
ApeKI GCWGC 8 cut(s) 400, 462, 1111, 1834, 2410, 2720, 2961, 3065
ApoI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
AspLEI GCGC 2 cut(s) 2504, 2961
AspS9I GGNCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
AsuC2I CCSGG 1 cut(s) 1615
AsuHPI GGTGA 4 cut(s) 377, 862, 1040, 1841
AsuII TTCGAA 1 cut(s) 1282
AsuNHI GCTAGC 1 cut(s) 2498
AvaII GGWCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
AxyI CCTNAGG 1 cut(s) 1554
BaeI ACNNNNGTAYC 2 cut(s) 2199, 2232
BalI TGGCCA 1 cut(s) 1055
BamHI GGATCC 2 cut(s) 293, 2705
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 1843, 2925
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 8 cut(s) 387, 449, 1098, 1821, 2397, 2707, 2948, 3052
BccI CCATC 7 cut(s) 155, 437, 772, 1192, 2126, 2860, 3092
BceAI ACGGC 3 cut(s) 586, 991, 2854
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 2 cut(s) 193, 2246
BcnI CCSGG 1 cut(s) 1615
BcoDI GTCTC 5 cut(s) 116, 1499, 2674, 2806, 3036
BfmI CTRYAG 4 cut(s) 272, 401, 669, 2931
BfoI RGCGCY 1 cut(s) 2962
BfuAI ACCTGC 1 cut(s) 2077
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 9 cut(s) 401, 463, 1112, 1835, 1900, 2411, 2721, 2962, 3066
BlsI GCNGC 9 cut(s) 402, 464, 1113, 1836, 1901, 2412, 2722, 2963, 3067
BmcAI AGTACT 1 cut(s) 2338
Bme1390I CCNGG 3 cut(s) 193, 1615, 2246
Bme18I GGWCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
BmeRI GACNNNNNGTC 2 cut(s) 274, 485
BmgT120I GGNCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
BmiI GGNNCC 5 cut(s) 295, 532, 877, 1690, 2707
BmrFI CCNGG 3 cut(s) 193, 1615, 2246
BmrI ACTGGG 2 cut(s) 1079, 2702
BmtI GCTAGC 1 cut(s) 2502
BmuI ACTGGG 2 cut(s) 1079, 2702
BpiI GAAGAC 2 cut(s) 1843, 2925
BpmI CTGGAG 1 cut(s) 1901
Bpu10I CCTNAGC 2 cut(s) 185, 2057
Bpu14I TTCGAA 1 cut(s) 1282
BpuEI CTTGAG 6 cut(s) 314, 412, 530, 1054, 2540, 3013
BpuMI CCSGG 1 cut(s) 1615
BsaAI YACGTR 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 298
BsaI GGTCTC 2 cut(s) 2806, 3036
BsaJI CCNNGG 6 cut(s) 793, 1342, 1614, 1684, 1990, 2245
BsaWI WCCGGW 1 cut(s) 872
BsaXI ACNNNNNCTCC 2 cut(s) 2078, 2108
Bse21I CCTNAGG 1 cut(s) 1554
Bse3DI GCAATG 1 cut(s) 2572
Bse8I GATNNNNATC 1 cut(s) 298
BseBI CCWGG 2 cut(s) 193, 2246
BseDI CCNNGG 6 cut(s) 793, 1342, 1614, 1684, 1990, 2245
BseGI GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2770, 2781
BseJI GATNNNNATC 1 cut(s) 298
BseMI GCAATG 1 cut(s) 2572
BseMII CTCAG 7 cut(s) 176, 822, 1477, 1493, 1770, 2868, 3029
BseRI GAGGAG 2 cut(s) 409, 1327
BseXI GCAGC 8 cut(s) 387, 449, 1098, 1821, 2397, 2707, 2948, 3052
BseYI CCCAGC 1 cut(s) 2020
BsgI GTGCAG 1 cut(s) 2706
BshFI GGCC 4 cut(s) 573, 855, 978, 1055
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 2 cut(s) 873, 1614
BslFI GGGAC 5 cut(s) 262, 720, 1702, 2188, 2709
BsmAI GTCTC 5 cut(s) 116, 1499, 2674, 2806, 3036
BsmBI CGTCTC 1 cut(s) 2674
BsmFI GGGAC 5 cut(s) 262, 720, 1702, 2188, 2709
BsmI GAATGC 2 cut(s) 1257, 3079
BsnI GGCC 4 cut(s) 573, 855, 978, 1055
Bso31I GGTCTC 2 cut(s) 2806, 3036
Bsp119I TTCGAA 1 cut(s) 1282
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
Bsp19I CCATGG 1 cut(s) 793
BspACI CCGC 2 cut(s) 1899, 1902
BspANI GGCC 4 cut(s) 573, 855, 978, 1055
BspCNI CTCAG 7 cut(s) 177, 821, 1476, 1492, 1769, 2869, 3030
BspLI GGNNCC 5 cut(s) 295, 532, 877, 1690, 2707
BspMAI CTGCAG 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 2077
BspOI GCTAGC 1 cut(s) 2502
BspPI GGATC 6 cut(s) 288, 301, 580, 1743, 2700, 2713
BspT104I TTCGAA 1 cut(s) 1282
BspTNI GGTCTC 2 cut(s) 2806, 3036
BsrDI GCAATG 1 cut(s) 2572
BssECI CCNNGG 6 cut(s) 793, 1342, 1614, 1684, 1990, 2245
BssMI GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
BssT1I CCWWGG 4 cut(s) 793, 1342, 1684, 1990
Bst2UI CCWGG 2 cut(s) 193, 2246
Bst4CI ACNGT 6 cut(s) 262, 276, 766, 2094, 2179, 2622
Bst6I CTCTTC 3 cut(s) 831, 2146, 2979
BstAPI GCANNNNNTGC 1 cut(s) 1896
BstBAI YACGTR 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 1282
BstC8I GCNNGC 4 cut(s) 800, 1892, 2500, 2872
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2770, 2781
BstH2I RGCGCY 1 cut(s) 2962
BstHHI GCGC 2 cut(s) 2504, 2961
BstKTI GATC 8 cut(s) 130, 296, 588, 1390, 1738, 2554, 2708, 3003
BstMAI GTCTC 5 cut(s) 116, 1499, 2674, 2806, 3036
BstMBI GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
BstMWI GCNNNNNNNGC 7 cut(s) 143, 152, 734, 1896, 2880, 2894, 3026
BstNI CCWGG 2 cut(s) 193, 2246
BstNSI RCATGY 5 cut(s) 1219, 1609, 2331, 2600, 2830
BstSCI CCNGG 3 cut(s) 191, 1613, 2244
BstSFI CTRYAG 4 cut(s) 272, 401, 669, 2931
BstV1I GCAGC 8 cut(s) 387, 449, 1098, 1821, 2397, 2707, 2948, 3052
BstV2I GAAGAC 2 cut(s) 1843, 2925
BstX2I RGATCY 3 cut(s) 293, 1735, 2705
BstXI CCANNNNNNTGG 4 cut(s) 628, 794, 1069, 1541
BstYI RGATCY 3 cut(s) 293, 1735, 2705
Bsu36I CCTNAGG 1 cut(s) 1554
BsuRI GGCC 4 cut(s) 573, 855, 978, 1055
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 7 cut(s) 611, 1113, 1185, 1203, 1401, 2770, 2781
BtsI GCAGTG 1 cut(s) 1515
BveI ACCTGC 1 cut(s) 2077
Cac8I GCNNGC 4 cut(s) 800, 1892, 2500, 2872
CaiI CAGNNNCTG 1 cut(s) 1225
CfoI GCGC 2 cut(s) 2504, 2961
Cfr13I GGNCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
CseI GACGC 1 cut(s) 1149
Csp6I GTAC 4 cut(s) 1367, 2160, 2208, 2337
CspCI CAANNNNNGTGG 4 cut(s) 1447, 1482, 1758, 1793
CviQI GTAC 4 cut(s) 1367, 2160, 2208, 2337
DpnI GATC 8 cut(s) 129, 295, 587, 1389, 1737, 2553, 2707, 3002
DpnII GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
DraI TTTAAA 1 cut(s) 2121
DriI GACNNNNNGTC 2 cut(s) 274, 485
EaeI YGGCCR 1 cut(s) 1053
Eam1104I CTCTTC 3 cut(s) 831, 2146, 2979
Eam1105I GACNNNNNGTC 2 cut(s) 274, 485
EarI CTCTTC 3 cut(s) 831, 2146, 2979
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 4 cut(s) 793, 1342, 1684, 1990
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 2 cut(s) 2806, 3036
Eco32I GATATC 1 cut(s) 1330
Eco47I GGWCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
Eco47III AGCGCT 1 cut(s) 2960
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 4 cut(s) 1421, 2127, 2453, 2673
Eco81I CCTNAGG 1 cut(s) 1554
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 1244
EcoRII CCWGG 2 cut(s) 191, 2244
EcoRV GATATC 1 cut(s) 1330
EcoT14I CCWWGG 4 cut(s) 793, 1342, 1684, 1990
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 4 cut(s) 793, 1342, 1684, 1990
Esp3I CGTCTC 1 cut(s) 2674
FalI AAGNNNNNCTT 2 cut(s) 2825, 2857
FaqI GGGAC 5 cut(s) 262, 720, 1702, 2188, 2709
FauNDI CATATG 1 cut(s) 2758
FblI GTMKAC 3 cut(s) 1163, 2181, 2753
Fnu4HI GCNGC 9 cut(s) 401, 463, 1112, 1835, 1900, 2411, 2721, 2962, 3066
FokI GGATG 7 cut(s) 598, 1120, 1172, 1210, 1388, 2768, 2777
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 9 cut(s) 401, 463, 1112, 1835, 1900, 2411, 2721, 2962, 3066
GlaI GCGC 2 cut(s) 2503, 2960
GluI GCNGC 9 cut(s) 401, 463, 1112, 1835, 1900, 2411, 2721, 2962, 3066
GsaI CCCAGC 1 cut(s) 2024
GsuI CTGGAG 1 cut(s) 1901
HaeII RGCGCY 1 cut(s) 2962
HaeIII GGCC 4 cut(s) 573, 855, 978, 1055
HapII CCGG 2 cut(s) 873, 1614
HgaI GACGC 1 cut(s) 1149
HhaI GCGC 2 cut(s) 2504, 2961
Hin6I GCGC 2 cut(s) 2502, 2959
HinP1I GCGC 2 cut(s) 2502, 2959
HincII GTYRAC 2 cut(s) 1164, 1213
HindII GTYRAC 2 cut(s) 1164, 1213
HindIII AAGCTT 7 cut(s) 5, 322, 623, 685, 1277, 1301, 2395
HpaII CCGG 2 cut(s) 873, 1614
HphI GGTGA 4 cut(s) 377, 862, 1040, 1841
Hpy166II GTNNAC 5 cut(s) 1164, 1213, 2182, 2754, 2802
Hpy188III TCNNGA 9 cut(s) 184, 547, 646, 1226, 1323, 1743, 2314, 2915, 3088
Hpy8I GTNNAC 5 cut(s) 1164, 1213, 2182, 2754, 2802
Hpy99I CGWCG 1 cut(s) 1165
HpyAV CCTTC 6 cut(s) 218, 603, 643, 1373, 1950, 2858
HpyCH4III ACNGT 6 cut(s) 262, 276, 766, 2094, 2179, 2622
HpyCH4IV ACGT 3 cut(s) 309, 1712, 2667
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 152, 734, 1896, 2880, 2894, 3026
HpySE526I ACGT 3 cut(s) 309, 1712, 2667
HspAI GCGC 2 cut(s) 2502, 2959
Kzo9I GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
LmnI GCTCC 4 cut(s) 194, 334, 470, 1962
Lsp1109I GCAGC 8 cut(s) 387, 449, 1098, 1821, 2397, 2707, 2948, 3052
MaeII ACGT 3 cut(s) 309, 1712, 2667
MaeIII GTNAC 8 cut(s) 206, 256, 386, 1028, 1359, 2238, 2437, 2656
MalI GATC 8 cut(s) 129, 295, 587, 1389, 1737, 2553, 2707, 3002
MboI GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
MfeI CAATTG 3 cut(s) 525, 633, 1790
MflI RGATCY 3 cut(s) 293, 1735, 2705
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 1055
MluNI TGGCCA 1 cut(s) 1055
MlyI GAGTC 2 cut(s) 1501, 3004
MmeI TCCRAC 6 cut(s) 246, 492, 750, 1649, 1822, 1861
Mox20I TGGCCA 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 1055
MseI TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2832
MslI CAYNNNNRTG 2 cut(s) 884, 1123
Msp20I TGGCCA 1 cut(s) 1055
MspI CCGG 2 cut(s) 873, 1614
MspR9I CCNGG 3 cut(s) 193, 1615, 2246
MunI CAATTG 3 cut(s) 525, 633, 1790
Mva1269I GAATGC 2 cut(s) 1257, 3079
MvaI CCWGG 2 cut(s) 193, 2246
MwoI GCNNNNNNNGC 7 cut(s) 143, 152, 734, 1896, 2880, 2894, 3026
NciI CCSGG 1 cut(s) 1615
NcoI CCATGG 1 cut(s) 793
NdeI CATATG 1 cut(s) 2758
NdeII GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
NheI GCTAGC 1 cut(s) 2498
NlaIV GGNNCC 5 cut(s) 295, 532, 877, 1690, 2707
NmeAIII GCCGAG 1 cut(s) 2296
NmuCI GTSAC 5 cut(s) 206, 256, 386, 1028, 2437
NspI RCATGY 5 cut(s) 1219, 1609, 2331, 2600, 2830
NspV TTCGAA 1 cut(s) 1282
PciI ACATGT 3 cut(s) 1605, 2596, 2826
PctI GAATGC 2 cut(s) 1257, 3079
PfeI GAWTC 9 cut(s) 200, 864, 1567, 1765, 1804, 1949, 2038, 2900, 3032
PflMI CCANNNNNTGG 3 cut(s) 286, 383, 2732
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 9 cut(s) 402, 464, 1113, 1836, 1901, 2412, 2722, 2963, 3067
PleI GAGTC 2 cut(s) 1500, 3003
PpsI GAGTC 2 cut(s) 1500, 3003
Ppu21I YACGTR 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 531
PscI ACATGT 3 cut(s) 1605, 2596, 2826
PsiI TTATAA 1 cut(s) 2321
Psp124BI GAGCTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 1712
Psp5II RGGWCCY 1 cut(s) 531
Psp6I CCWGG 2 cut(s) 191, 2244
PspFI CCCAGC 1 cut(s) 2020
PspGI CCWGG 2 cut(s) 191, 2244
PspN4I GGNNCC 5 cut(s) 295, 532, 877, 1690, 2707
PspPI GGNCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
PspPPI RGGWCCY 1 cut(s) 531
PsrI GAACNNNNNNTAC 2 cut(s) 649, 681
PstI CTGCAG 1 cut(s) 405
PstNI CAGNNNCTG 1 cut(s) 1225
PsuI RGATCY 3 cut(s) 293, 1735, 2705
RsaI GTAC 4 cut(s) 1368, 2161, 2209, 2338
RsaNI GTAC 4 cut(s) 1367, 2160, 2208, 2337
RseI CAYNNNNRTG 2 cut(s) 884, 1123
SacI GAGCTC 1 cut(s) 191
SalI GTCGAC 1 cut(s) 1162
SaqAI TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2832
SatI GCNGC 9 cut(s) 401, 463, 1112, 1835, 1900, 2411, 2721, 2962, 3066
Sau3AI GATC 8 cut(s) 127, 293, 585, 1387, 1735, 2551, 2705, 3000
Sau96I GGNCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
ScaI AGTACT 1 cut(s) 2338
SchI GAGTC 2 cut(s) 1501, 3004
ScrFI CCNGG 3 cut(s) 193, 1615, 2246
SduI GDGCHC 1 cut(s) 191
SfcI CTRYAG 4 cut(s) 272, 401, 669, 2931
SfuI TTCGAA 1 cut(s) 1282
SinI GGWCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
SmiMI CAYNNNNRTG 2 cut(s) 884, 1123
SmlI CTYRAG 6 cut(s) 329, 391, 545, 1033, 2555, 2992
SmoI CTYRAG 6 cut(s) 329, 391, 545, 1033, 2555, 2992
SsiI CCGC 2 cut(s) 1899, 1902
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 3 cut(s) 191, 1613, 2244
StyI CCWWGG 4 cut(s) 793, 1342, 1684, 1990
TaaI ACNGT 6 cut(s) 262, 276, 766, 2094, 2179, 2622
TaiI ACGT 3 cut(s) 312, 1715, 2670
TaqI TCGA 6 cut(s) 130, 1163, 1282, 1744, 1824, 2302
TaqII GACCGA 2 cut(s) 169, 3060
TatI WGTACW 1 cut(s) 2336
TauI GCSGC 1 cut(s) 1902
TfiI GAWTC 9 cut(s) 200, 864, 1567, 1765, 1804, 1949, 2038, 2900, 3032
Tru1I TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2832
Tru9I TTAA 7 cut(s) 410, 1352, 1475, 2045, 2120, 2232, 2832
TseFI GTSAC 5 cut(s) 206, 256, 386, 1028, 2437
TseI GCWGC 8 cut(s) 400, 462, 1111, 1834, 2410, 2720, 2961, 3065
Tsp45I GTSAC 5 cut(s) 206, 256, 386, 1028, 2437
TspGWI ACGGA 1 cut(s) 876
Van91I CCANNNNNTGG 3 cut(s) 286, 383, 2732
VpaK11BI GGWCC 6 cut(s) 181, 268, 531, 875, 1591, 1689
XapI RAATTY 5 cut(s) 513, 986, 1244, 1285, 1620
XceI RCATGY 5 cut(s) 1219, 1609, 2331, 2600, 2830
XmiI GTMKAC 3 cut(s) 1163, 2181, 2753
ZrmI AGTACT 1 cut(s) 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.