MD00G1000300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016858
Physical Location & Seq
Reverse (-)
43335 .. 46922
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1000300.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGCCGAACCACTGAGTAGTCCTGAAGTTGAAACTAGTACTGTGCAGCAAGAGTTGATCAGATCAGCAGCGGTCGAAATTGCGTATTTAGCGGGTGATGATATTACTACTCCAGATGTGCATGGGGCGGGCATGAAAATTGAGAGGGCAAGGGATGCATACAAAGTATATGCAGGGTTTGAAGAGAAGCCTAACAAGGTGGAGATTTTGAGTTGGTATTTTTATGAGTTGTGCTCCCACTTCATTCATCTTGTTCTTATCCCGATCGTCTTCCCTCTCATCATTACCCAAATCGGAGACGCCGCACGCGACTCTGGGTCTGGGGTGGGGTTAGCCCGCAAGTACAAGGGCGTGGCCGCTTGCACTCACAAGGAAACCATACTGTACCTTAGACTCACAAACAGGTCATTACTCTCCAAATACTCACCTCTAGAGTGGACTTCAATTTCATGGGGTATAGGTCTAGCTCTAGCTGCTCCACTCCTTCACTTTGTCTCCATCATCCTTGACCATGGCTACAAGCAAACCCTCGTTGCCGGCGCCGCAGCAGCCATCGGGTCCATCTTCTGTCTTCCAGCTGGCTTTTTCCGAAACATCTGGCTCTTTCCACCATACATTGCCACCATAGTTTGTGCCAACGTCATTGCCACCGCCTGCCACACCCGCCACCTCGGCCTATTGGTCCGCGGTTTCACGGGACCCACTATCCGAAAATCCCAATTCCAAGCCCGAAGAGCAGTCTCCGCCTGGCTTTCGCTCTACGCAGCCGCGGCGGGCTCATTAGGCTCCGCCGTCTTCTCATCCTTCACTTACCACATGCTTAGGGAAGATGAAGAGTTACTAGGGCTTTGGATTGTGTCAATCTTCAGTGGGATCATATGGTTAACTGGGATATTCCATTTCTTTGTGACTAATAGGCCCTGTGTGAATCCACCTATCTCAAAGTCCCATGCACTTTCAGTCTTCAAGTTTCCTCATGCAGTTGGAGGACTAATGGGAACTTTCCTCTCCTCGTTCACATCAATGTGTATTTTCTCAGGGGGAGTGCTTTACCTAGTTGGGCAGCTTTGCTATGATCCAAAAGCATTGCTATATTTTTTCCTAACTTACTTCATCTTCCCTTTGATTTCTCTCCCAATGTTACAACCACTGCAGCATCTCATCAAGGCGGACGCCATCAAAATGCAACTCATGGGTTTCTTCCTATCAGCACTCACTTCCGGGTCCGGATTCTATTTCCGGAACTCACTTTGGGGGCATCGTCATGTCCTGTTCTTCGGTGCGGTTCAAAGCACGTCCGCGGGGCTTTTGCACGCCTTTGGACGGGTTCTGCTGATGGATTGTTCGCCGCCAGGGAAAGAAGGCGCTTTCTCTACATGGCATTCCTGGATCAAAGCTCTTGGCACATGTGCTGGCTTCGCATTGGCAACGGCAGTCCCAGGAAACCTGTTCACTTCTTTCGGGCTGTCATTTTTAACCACTGTGTGTGGAGTGGTGATTCTGATTTTTGGGAACGTCAGCGATTATGGAGGAGCCAAAGCTGCCGGCCATGTGAGGCACGACAGCGAGCGAGGAGGCTCGCCTGTAAGTGGCCTTGAGGCTGCCTATGAGACACAAGAACCAGCAGCTGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

546

Amino Acids

59.21

Weight (kDa)

8.54

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016858)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g23600
malus_domestica MD00G1000300.v1.1
prunus_persica Prupe.5G024200_v2.0.a1
pyrus_communis pycom16g25370
rosa_chinensis RchiOBHm_Chr7g0212901
rosa_laevigata RLG00000002857
rosa_multiflora Rmu_ssc0000491.1_g000003
rosa_roxburghii Rroxscaffold_3G00246440
rosa_rugosa Rorug07G0137500
rosa_samantha Rh7AG269100 Rh7BG264700 Rh7CG288300 Rh7DG277300
rosa_wichuraiana Rw7G023380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 539
AccII CGCG 4 cut(s) 309, 687, 770, 1301
AccIII TCCGGA 2 cut(s) 1226, 1239
AclWI GGATC 3 cut(s) 881, 1070, 1397
AcoI YGGCCR 3 cut(s) 3, 354, 1546
AcuI CTGAAG 2 cut(s) 45, 850
AcyI GRCGYC 3 cut(s) 300, 540, 1173
AdeI CACNNNGTG 1 cut(s) 1485
AfaI GTAC 4 cut(s) 40, 344, 386, 1632
AfiI CCNNNNNNNGG 2 cut(s) 1323, 1589
AflIII ACRYGT 1 cut(s) 1406
AgsI TTSAA 5 cut(s) 32, 182, 444, 967, 1289
AhdI GACNNNNNGTC 1 cut(s) 316
AhlI ACTAGT 1 cut(s) 35
AjiI CACGTC 1 cut(s) 1296
AjnI CCWGG 4 cut(s) 746, 1351, 1385, 1438
AluBI AGCT 7 cut(s) 467, 473, 578, 1066, 1397, 1541, 1628
AluI AGCT 7 cut(s) 467, 473, 578, 1066, 1397, 1541, 1628
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 4 cut(s) 291, 499, 745, 1604
AlwI GGATC 3 cut(s) 881, 1070, 1397
AlwNI CAGNNNCTG 1 cut(s) 1628
Aor13HI TCCGGA 2 cut(s) 1226, 1239
AoxI GGCC 6 cut(s) 3, 354, 673, 917, 1546, 1591
AspLEI GCGC 2 cut(s) 542, 1367
AspS9I GGNCC 5 cut(s) 558, 682, 698, 918, 1224
AsuC2I CCSGG 1 cut(s) 1222
AsuHPI GGTGA 3 cut(s) 107, 417, 1507
AvaII GGWCC 4 cut(s) 558, 682, 698, 1224
BanI GGYRCC 1 cut(s) 539
BanII GRGCYC 1 cut(s) 779
BbsI GAAGAC 4 cut(s) 262, 563, 787, 955
Bbv12I GWGCWC 1 cut(s) 236
BccI CCATC 5 cut(s) 506, 560, 569, 1184, 1330
BceAI ACGGC 2 cut(s) 776, 1446
BciT130I CCWGG 4 cut(s) 748, 1353, 1387, 1440
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 1222
BcoDI GTCTC 4 cut(s) 291, 499, 745, 1604
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 6 cut(s) 36, 431, 464, 470, 842, 1055
BfmI CTRYAG 1 cut(s) 1151
BfoI RGCGCY 2 cut(s) 543, 1368
BglI GCCNNNNNGGC 1 cut(s) 672
BmcAI AGTACT 1 cut(s) 40
Bme1390I CCNGG 5 cut(s) 748, 1222, 1353, 1387, 1440
Bme18I GGWCC 4 cut(s) 558, 682, 698, 1224
BmeRI GACNNNNNGTC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 1296
BmgT120I GGNCC 5 cut(s) 558, 682, 698, 918, 1224
BmiI GGNNCC 7 cut(s) 541, 559, 699, 700, 787, 1225, 1534
BmrFI CCNGG 5 cut(s) 748, 1222, 1353, 1387, 1440
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 3 cut(s) 145, 1165, 1267
BmuI ACTGGG 1 cut(s) 897
BpiI GAAGAC 4 cut(s) 262, 563, 787, 955
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 821
BpuEI CTTGAG 1 cut(s) 1616
BpuMI CCSGG 1 cut(s) 1222
BsaHI GRCGYC 3 cut(s) 300, 540, 1173
BsaJI CCNNGG 7 cut(s) 511, 670, 685, 768, 1299, 1352, 1438
BsaWI WCCGGW 2 cut(s) 1226, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 1323, 1589
Bse118I RCCGGY 2 cut(s) 536, 1544
Bse1I ACTGG 1 cut(s) 892
Bse3DI GCAATG 3 cut(s) 615, 642, 1085
BseAI TCCGGA 2 cut(s) 1226, 1239
BseBI CCWGG 4 cut(s) 748, 1353, 1387, 1440
BseDI CCNNGG 7 cut(s) 511, 670, 685, 768, 1299, 1352, 1438
BseGI GGATG 3 cut(s) 160, 501, 800
BseLI CCNNNNNNNGG 2 cut(s) 1323, 1589
BseMI GCAATG 3 cut(s) 615, 642, 1085
BseMII CTCAG 2 cut(s) 5, 1050
BseNI ACTGG 1 cut(s) 892
BseRI GAGGAG 3 cut(s) 1000, 1545, 1587
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 4 cut(s) 309, 687, 770, 1301
Bsh1285I CGRYCG 2 cut(s) 75, 267
BshFI GGCC 6 cut(s) 5, 356, 675, 919, 1548, 1593
BshNI GGYRCC 1 cut(s) 539
BsiEI CGRYCG 2 cut(s) 75, 267
BsiHKAI GWGCWC 1 cut(s) 236
BsiSI CCGG 5 cut(s) 537, 1221, 1227, 1240, 1545
BslFI GGGAC 3 cut(s) 711, 931, 1421
BslI CCNNNNNNNGG 2 cut(s) 1323, 1589
BsmAI GTCTC 4 cut(s) 291, 499, 745, 1604
BsmBI CGTCTC 1 cut(s) 291
BsmFI GGGAC 3 cut(s) 711, 931, 1421
BsmI GAATGC 1 cut(s) 1381
BsnI GGCC 6 cut(s) 5, 356, 675, 919, 1548, 1593
Bsp1286I GDGCHC 2 cut(s) 236, 779
Bsp13I TCCGGA 2 cut(s) 1226, 1239
Bsp1407I TGTACA 1 cut(s) 1630
Bsp143I GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
Bsp19I CCATGG 1 cut(s) 511
BspANI GGCC 6 cut(s) 5, 356, 675, 919, 1548, 1593
BspCNI CTCAG 2 cut(s) 6, 1049
BspEI TCCGGA 2 cut(s) 1226, 1239
BspFNI CGCG 4 cut(s) 309, 687, 770, 1301
BspLI GGNNCC 7 cut(s) 541, 559, 699, 700, 787, 1225, 1534
BspMAI CTGCAG 1 cut(s) 1155
BspPI GGATC 3 cut(s) 881, 1070, 1397
BspQI GCTCTTC 1 cut(s) 727
BspT107I GGYRCC 1 cut(s) 539
BsrDI GCAATG 3 cut(s) 615, 642, 1085
BsrFI RCCGGY 2 cut(s) 536, 1544
BsrGI TGTACA 1 cut(s) 1630
BsrI ACTGG 1 cut(s) 892
BssAI RCCGGY 2 cut(s) 536, 1544
BssECI CCNNGG 7 cut(s) 511, 670, 685, 768, 1299, 1352, 1438
BssMI GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
BssNI GRCGYC 3 cut(s) 300, 540, 1173
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 4 cut(s) 748, 1353, 1387, 1440
Bst4CI ACNGT 3 cut(s) 43, 384, 1483
Bst6I CTCTTC 3 cut(s) 177, 727, 828
BstACI GRCGYC 3 cut(s) 300, 540, 1173
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 1630
BstDEI CTNAG 4 cut(s) 14, 389, 821, 1036
BstDSI CCRYGG 4 cut(s) 511, 685, 768, 1299
BstF5I GGATG 3 cut(s) 160, 501, 800
BstFNI CGCG 4 cut(s) 309, 687, 770, 1301
BstH2I RGCGCY 2 cut(s) 543, 1368
BstHHI GCGC 2 cut(s) 542, 1367
BstKTI GATC 6 cut(s) 60, 65, 267, 876, 1078, 1392
BstMAI GTCTC 4 cut(s) 291, 499, 745, 1604
BstMBI GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
BstMCI CGRYCG 2 cut(s) 75, 267
BstNI CCWGG 4 cut(s) 748, 1353, 1387, 1440
BstNSI RCATGY 2 cut(s) 820, 1410
BstSCI CCNGG 5 cut(s) 746, 1220, 1351, 1385, 1438
BstSFI CTRYAG 1 cut(s) 1151
BstUI CGCG 4 cut(s) 309, 687, 770, 1301
BstV2I GAAGAC 4 cut(s) 262, 563, 787, 955
BsuRI GGCC 6 cut(s) 5, 356, 675, 919, 1548, 1593
BtgI CCRYGG 4 cut(s) 511, 685, 768, 1299
BtrI CACGTC 1 cut(s) 1296
BtsCI GGATG 3 cut(s) 160, 501, 800
BtsI GCAGTG 1 cut(s) 1148
BtsIMutI CAGTG 4 cut(s) 11, 874, 1148, 1479
CaiI CAGNNNCTG 1 cut(s) 1628
CfoI GCGC 2 cut(s) 542, 1367
Cfr10I RCCGGY 2 cut(s) 536, 1544
Cfr13I GGNCC 5 cut(s) 558, 682, 698, 918, 1224
Cfr42I CCGCGG 3 cut(s) 688, 771, 1302
CseI GACGC 2 cut(s) 308, 1181
Csp6I GTAC 4 cut(s) 39, 343, 385, 1631
CviQI GTAC 4 cut(s) 39, 343, 385, 1631
DdeI CTNAG 4 cut(s) 14, 389, 821, 1036
DinI GGCGCC 1 cut(s) 541
DpnI GATC 6 cut(s) 59, 64, 266, 875, 1077, 1391
DpnII GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
DraIII CACNNNGTG 1 cut(s) 1485
DriI GACNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 3 cut(s) 3, 354, 1546
Eam1104I CTCTTC 3 cut(s) 177, 727, 828
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 3 cut(s) 177, 727, 828
EciI GGCGGA 3 cut(s) 733, 778, 1184
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 779
Eco47I GGWCC 4 cut(s) 558, 682, 698, 1224
Eco57I CTGAAG 2 cut(s) 45, 850
EcoO109I RGGNCCY 2 cut(s) 698, 918
EcoRII CCWGG 4 cut(s) 746, 1351, 1385, 1438
EcoT14I CCWWGG 1 cut(s) 511
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 779
EgeI GGCGCC 1 cut(s) 541
EheI GGCGCC 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 291
FaqI GGGAC 3 cut(s) 711, 931, 1421
FauI CCCGC 6 cut(s) 85, 121, 344, 671, 766, 1294
FauNDI CATATG 1 cut(s) 878
FbaI TGATCA 1 cut(s) 57
FokI GGATG 3 cut(s) 167, 488, 787
FriOI GRGCYC 1 cut(s) 779
FspBI CTAG 6 cut(s) 36, 431, 464, 470, 842, 1055
GlaI GCGC 2 cut(s) 541, 1366
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 2 cut(s) 543, 1368
HaeIII GGCC 6 cut(s) 5, 356, 675, 919, 1548, 1593
HapII CCGG 5 cut(s) 537, 1221, 1227, 1240, 1545
HgaI GACGC 2 cut(s) 308, 1181
HhaI GCGC 2 cut(s) 542, 1367
Hin1I GRCGYC 3 cut(s) 300, 540, 1173
Hin6I GCGC 2 cut(s) 540, 1365
HinP1I GCGC 2 cut(s) 540, 1365
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 5 cut(s) 311, 393, 928, 1230, 1498
HpaI GTTAAC 1 cut(s) 885
HpaII CCGG 5 cut(s) 537, 1221, 1227, 1240, 1545
HphI GGTGA 3 cut(s) 107, 417, 1507
Hpy166II GTNNAC 5 cut(s) 438, 885, 1017, 1452, 1633
Hpy188I TCNGA 5 cut(s) 62, 296, 590, 710, 1503
Hpy188III TCNNGA 6 cut(s) 23, 113, 262, 431, 1227, 1240
Hpy8I GTNNAC 5 cut(s) 438, 885, 1017, 1452, 1633
HpyAV CCTTC 3 cut(s) 494, 814, 1355
HpyCH4III ACNGT 3 cut(s) 43, 384, 1483
HpyCH4IV ACGT 3 cut(s) 639, 1295, 1515
HpyF3I CTNAG 4 cut(s) 14, 389, 821, 1036
HpySE526I ACGT 3 cut(s) 639, 1295, 1515
Hsp92I GRCGYC 3 cut(s) 300, 540, 1173
HspAI GCGC 2 cut(s) 540, 1365
KasI GGCGCC 1 cut(s) 539
KflI GGGWCCC 1 cut(s) 698
Kpn2I TCCGGA 2 cut(s) 1226, 1239
KroI GCCGGC 2 cut(s) 536, 1544
KroNI GCCGGC 2 cut(s) 538, 1546
Ksp22I TGATCA 1 cut(s) 57
KspAI GTTAAC 1 cut(s) 885
KspI CCGCGG 3 cut(s) 688, 771, 1302
Kzo9I GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 4 cut(s) 239, 481, 791, 1532
LweI GCATC 3 cut(s) 145, 1165, 1267
MaeI CTAG 6 cut(s) 36, 431, 464, 470, 842, 1055
MaeII ACGT 3 cut(s) 639, 1295, 1515
MaeIII GTNAC 3 cut(s) 837, 907, 1140
MalI GATC 6 cut(s) 59, 64, 266, 875, 1077, 1391
MboI GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
MhlI GDGCHC 2 cut(s) 236, 779
MluCI AATT 4 cut(s) 78, 138, 444, 719
Mly113I GGCGCC 1 cut(s) 540
MlyI GAGTC 2 cut(s) 305, 387
MmeI TCCRAC 1 cut(s) 964
Mph1103I ATGCAT 1 cut(s) 160
MroI TCCGGA 2 cut(s) 1226, 1239
MroNI GCCGGC 2 cut(s) 536, 1544
MseI TTAA 2 cut(s) 884, 1475
MslI CAYNNNNRTG 4 cut(s) 1022, 1024, 1181, 1263
MspA1I CMGCKG 6 cut(s) 71, 578, 687, 770, 1301, 1628
MspI CCGG 5 cut(s) 537, 1221, 1227, 1240, 1545
MspR9I CCNGG 5 cut(s) 748, 1222, 1353, 1387, 1440
Mva1269I GAATGC 1 cut(s) 1381
MvaI CCWGG 4 cut(s) 748, 1353, 1387, 1440
MvnI CGCG 4 cut(s) 309, 687, 770, 1301
NaeI GCCGGC 2 cut(s) 538, 1546
NarI GGCGCC 1 cut(s) 540
NciI CCSGG 1 cut(s) 1222
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 1 cut(s) 878
NdeII GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
NgoMIV GCCGGC 2 cut(s) 536, 1544
NlaIV GGNNCC 7 cut(s) 541, 559, 699, 700, 787, 1225, 1534
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 1 cut(s) 907
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 2 cut(s) 820, 1410
PciI ACATGT 1 cut(s) 1406
PciSI GCTCTTC 1 cut(s) 727
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 1 cut(s) 1381
PdiI GCCGGC 2 cut(s) 538, 1546
PfeI GAWTC 3 cut(s) 928, 1230, 1498
PfoI TCCNGGA 1 cut(s) 1385
Ple19I CGATCG 1 cut(s) 267
PleI GAGTC 2 cut(s) 305, 387
PluTI GGCGCC 1 cut(s) 543
PpsI GAGTC 2 cut(s) 305, 387
PpuMI RGGWCCY 1 cut(s) 698
PscI ACATGT 1 cut(s) 1406
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 4 cut(s) 746, 1351, 1385, 1438
PspGI CCWGG 4 cut(s) 746, 1351, 1385, 1438
PspN4I GGNNCC 7 cut(s) 541, 559, 699, 700, 787, 1225, 1534
PspPI GGNCC 5 cut(s) 558, 682, 698, 918, 1224
PspPPI RGGWCCY 1 cut(s) 698
PstI CTGCAG 1 cut(s) 1155
PstNI CAGNNNCTG 1 cut(s) 1628
PvuI CGATCG 1 cut(s) 267
PvuII CAGCTG 2 cut(s) 578, 1628
RsaI GTAC 4 cut(s) 40, 344, 386, 1632
RsaNI GTAC 4 cut(s) 39, 343, 385, 1631
RseI CAYNNNNRTG 4 cut(s) 1022, 1024, 1181, 1263
SacII CCGCGG 3 cut(s) 688, 771, 1302
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 2 cut(s) 884, 1475
Sau3AI GATC 6 cut(s) 57, 62, 264, 873, 1075, 1389
Sau96I GGNCC 5 cut(s) 558, 682, 698, 918, 1224
ScaI AGTACT 1 cut(s) 40
SchI GAGTC 2 cut(s) 305, 387
ScrFI CCNGG 5 cut(s) 748, 1222, 1353, 1387, 1440
SduI GDGCHC 2 cut(s) 236, 779
SfaNI GCATC 3 cut(s) 145, 1165, 1267
SfcI CTRYAG 1 cut(s) 1151
SfoI GGCGCC 1 cut(s) 541
Sfr303I CCGCGG 3 cut(s) 688, 771, 1302
SgrBI CCGCGG 3 cut(s) 688, 771, 1302
SinI GGWCC 4 cut(s) 558, 682, 698, 1224
SmiMI CAYNNNNRTG 4 cut(s) 1022, 1024, 1181, 1263
SmlI CTYRAG 1 cut(s) 1595
SmoI CTYRAG 1 cut(s) 1595
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 4 cut(s) 78, 138, 444, 719
SspDI GGCGCC 1 cut(s) 539
SspMI CTAG 6 cut(s) 36, 431, 464, 470, 842, 1055
StyD4I CCNGG 5 cut(s) 746, 1220, 1351, 1385, 1438
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 43, 384, 1483
TaiI ACGT 3 cut(s) 642, 1298, 1518
TaqI TCGA 1 cut(s) 75
TasI AATT 4 cut(s) 78, 138, 444, 719
TatI WGTACW 3 cut(s) 38, 342, 1630
TauI GCSGC 6 cut(s) 305, 359, 545, 770, 773, 1351
TfiI GAWTC 3 cut(s) 928, 1230, 1498
Tru1I TTAA 2 cut(s) 884, 1475
Tru9I TTAA 2 cut(s) 884, 1475
TscAI CASTG 4 cut(s) 18, 874, 1155, 1486
TseFI GTSAC 1 cut(s) 907
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 6 cut(s) 149, 232, 236, 438, 846, 1102
TspRI CASTG 4 cut(s) 18, 874, 1155, 1486
VpaK11BI GGWCC 4 cut(s) 558, 682, 698, 1224
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 2 cut(s) 820, 1410
XspI CTAG 6 cut(s) 36, 431, 464, 470, 842, 1055
ZrmI AGTACT 1 cut(s) 40
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.